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1.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 33(4): 365-372, 2021 Aug 19.
Artigo em Chinês | MEDLINE | ID: mdl-34505443

RESUMO

OBJECTIVE: To investigate the current distribution of ticks and predict the suitable habitats of ticks in the Yangtze River Delta urban agglomeration in 2017, so as to provide insights into tick control and management of tick-borne diseases in these areas. METHODS: All publications pertaining to tick and pathogen distribution in the Yangtze River Delta urban agglomeration were retrieved, and the geographical location of tick distribution was extracted. The effects of 19 climatic factors on the distribution of ticks were examined using the jackknife method, including the mean temperature of the wettest quarter, precipitation of the coldest quarter, mean temperature of the driest quarter, maximum temperature of the warmest month, precipitation of the driest month, minimal temperature of the coldest month, annual precipitation, mean daily temperature range, precipitation seasonality, annual temperature range, temperature seasonality, annual mean temperature, mean temperature of the warmest quarter, precipitation of the wettest quarter, isothermality, mean temperature of the coldest quarter, precipitation of the wettest month, precipitation of the driest quarter and precipitation of the warmest quarter. The distribution of ticks was analyzed in 2020 using the maximum entropy (MaxEnt) model, and the potential suitable habitats of ticks were predicted in 2070 using the MaxEnt model based on climatic data. RESULTS: A total of 380 Chinese and English literatures were retrieved, and 148 tick distribution sites were extracted, with 135 sites included in the subsequent analysis. There were 7 genera (Haemaphysalis, Rhipicephalus, Ixodes, Dermacentor, Boophilus, Hyalomma and Amblyomma) and 27 species of ticks detected in the Yangtze River Delta urban agglomeration. The climatic factors affecting the distribution of ticks in the Yangtze River Delta urban agglomeration mainly included the mean temperature of the wettest quarter and the precipitation of the coldest quarter, with 26.1% and 23.6% contributions to tick distributions. The high-, medium- and low-suitable habitats of ticks were 20 337.08, 40 017.38 km2 and 74 931.43 km2 in the Yangtze River Delta urban agglomeration in 2020, respectively. The climate changes led to south expansion of the suitable habitats of ticks in the Yangtze River Delta urban agglomeration in 2070, and the total areas of suitable habitats of ticks was predicted to increase by 18 100 km2. In addition, the high-, medium- and low-suitable habitats of ticks were predicted to increase to 24 317.84, 45 283.02 km2 and 83 766.38 km2 in the Yangtze River Delta urban agglomeration in 2070, respectively. CONCLUSIONS: Multiple tick species are widespread in the Yangtze River Delta urban agglomeration, and the future climate changes may lead to expansion of tick distribution in these areas.


Assuntos
Ixodes , Rios , Animais , Mudança Climática , Ecossistema , Temperatura
2.
Artigo em Chinês | MEDLINE | ID: mdl-31495120

RESUMO

Objective: To establish a method for the determination ofsamarium oxide and lanthanum oxide by inductively coupled plasmamass spectrometryin the air of workplace. Methods: Samarium, lanthanum and their compounds in the air of workplace were collected through microporous filter. The samples were digested by nitricacid and perhydrol (V/V=4∶1) and detected by inductively coupled plasmamass spectrometry. Results: The linear range ofsamarium oxide and lanthanum oxide was 0-50.00 µg/L, Sm(2)O(3): y=0.0119x, r=0.9999; La(2)O(3): y=0.0617x, r=0.9998. The detection limits were less than 0.1 µg/L, and the minimum detection concentration were less than 1.52×10(-5) mg/m(3). The sampling efficiency were 100%, the recovery rates were 95.70%-102.01%, and the precision were 0.78%-1.58%. Conclusion: The indicators established in this study are conformed with the requirements of Chinese Occupational Standars of GBZ/T 210.4-2008, "The Guidelines for the Development of Occupational Hygiene StandarsMehods Part 4: Determination of Chemical Substances in the Air of Workplace".


Assuntos
Poluentes Ocupacionais do Ar/análise , Lantânio/análise , Óxidos/análise , Samário/análise , Espectrometria de Massas , Local de Trabalho
3.
J Photochem Photobiol B ; 181: 143-149, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29567315

RESUMO

Metronomic photodynamic therapy (mPDT) was developed to improve tumor-specific responses through cell death by apoptosis. We developed an mPDT suppository kit including ALA and LED suppositories and analyzed its killing effect on rectal tumors in rabbits. METHODS: The ALA (10 wt%) suppository was prepared using ALA powder, type 36 semi-synthetic fatty acid glyceride, and azone. The LED suppository was constructed by encapsulating LED units and a circuit in transparent epoxy resin. VX2 cells were injected into the rectal submucosa of rabbits to establish a carcinoma model in situ. The ALA suppository was inserted into the rectal cavity for 30 min of uptake and activated for 1 h by the LED suppository at a power density of 20 mW/cm2. The mPDT process was repeated three times once a day. MRI was used to monitor tumor growth, histopathology and TUNEL staining were performed at 14 days after mPDT. RESULTS: The overall response rate was 60% in the mPDT group using the kit in which the tumor size was decreased up to about 50% at 7 days post-mPDT and almost eliminated at 14 days. HE staining showed that only 6.16% of the tumor tissue remained after mPDT treatment. TUNEL detection showed that the apoptosis rate was 18.9%. CONCLUSION: We verified the killing effect of the mPDT suppository kit on rectal tumors in rabbits based on mPDT that induced tumor cell apoptosis.


Assuntos
Apoptose/efeitos dos fármacos , Fármacos Fotossensibilizantes/toxicidade , Supositórios/química , Ácido Aminolevulínico/toxicidade , Animais , Apoptose/efeitos da radiação , Linhagem Celular Tumoral , Luz , Imageamento por Ressonância Magnética , Fotoquimioterapia , Fármacos Fotossensibilizantes/uso terapêutico , Coelhos , Neoplasias Retais/diagnóstico por imagem , Neoplasias Retais/tratamento farmacológico , Neoplasias Retais/patologia , Transplante Homólogo
4.
J Appl Microbiol ; 123(6): 1447-1460, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28801977

RESUMO

AIMS: Lignin is an aromatic heteropolymer forming a physical barrier and it is a big challenge in biomass utilization. This paper first investigated lignin-degradation bacteria from rotten wood in Qinling Mountain. METHODS AND RESULTS: Nineteen potential strains were selected and ligninolytic enzyme activities were determined over 84 h. Strains that had higher enzyme activities were selected. Further, the biodegradation of wheat straw lignin and alkali lignin was evaluated indicating that Burkholderia sp. H1 had the highest capability. It was confirmed by gel permeation chromatography and field emission scanning electron microscope that alkali lignin was depolymerized into small fragments. The degraded products were analysed using gas chromatography-mass spectrometry. The total ion chromatograph of products treated for 7 days showed the formation of aromatic compounds, an important intermediate from lignin degradation. Interestingly, they disappeared in 15 days while the aldehyde and ester compounds increased. CONCLUSIONS: The results suggest that the lignin-degrading bacteria are abundant in rotten wood and strain H1 has high potential to break down lignin. SIGNIFICANCE AND IMPACT OF THE STUDY: The diversity of lignin-degrading bacteria in Qinling Mountain is revealed. The study of Burkholderia sp. H1 expands the range of bacteria for lignin degradation and provides novel bacteria for application to lignocellulosic biomass.


Assuntos
Bactérias/isolamento & purificação , Bactérias/metabolismo , Lignina/metabolismo , Madeira/microbiologia , Bactérias/classificação , Bactérias/genética , Biodegradação Ambiental , Biomassa , China , Cromatografia Gasosa-Espectrometria de Massas , Triticum/metabolismo , Triticum/microbiologia
5.
Reprod Domest Anim ; 51(1): 135-42, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26698926

RESUMO

The effects of dietary ß-hydroxy-ß-methylbutyrate (HMB) supplementation during gestation on reproductive performance of sows and the mRNA expression of myogenic markers in skeletal muscle of neonatal pigs were determined. At day 35 of gestation, a total of 20 sows (Landrace × Yorkshire, at third parity) were randomly assigned to two groups, with each group receiving either a basal diet or the same diet supplemented with 4 g/day ß-hydroxy-ß-methylbutyrate calcium (HMB-Ca) until parturition. At parturition, the total and live litter size were not markedly different between treatments, however, the sows fed HMB diet had a decreased rate of stillborn piglets compared with the sows fed the control (CON) diets (p < 0.05). In addition, piglets from the sows fed HMB diet tended to have an increased birth weight (p = 0.08), and a reduced rate of low birth weight piglets (p = 0.05) compared with piglets from the CON sows. Nevertheless, lower feed intake during lactation was observed in the sows fed the HMB diet compared with those on the CON diet (p < 0.01). The relative weights of the longissimus dorsi (LD) and semitendinosus (ST) muscle were higher (p < 0.05) in neonatal pigs from the HMB than the CON sows. Furthermore, maternal HMB treatment increased the mRNA levels of the myogenic genes, including muscle regulatory factor-4 (MRF4, p < 0.05), myogenic differentiation factor (MyoD) and insulin-like growth factor-1 (IGF-1, p < 0.01). In conclusion, dietary HMB supplementation to sows at 4 g/day from day 35 of gestation to term significantly improves pregnancy outcomes and increases the expression of myogenic genes in skeletal muscle of neonatal piglets, but reduces feed intake of sows during lactation.


Assuntos
Animais Recém-Nascidos/metabolismo , Biomarcadores/análise , Desenvolvimento Muscular/genética , Músculo Esquelético/química , Sus scrofa/fisiologia , Valeratos/administração & dosagem , Animais , Dieta/veterinária , Feminino , Expressão Gênica/efeitos dos fármacos , Idade Gestacional , Tamanho da Ninhada de Vivíparos/efeitos dos fármacos , Gravidez , Resultado da Gravidez , RNA Mensageiro/análise , Natimorto/epidemiologia , Natimorto/veterinária , Suínos , Doenças dos Suínos/epidemiologia
6.
Lasers Med Sci ; 30(1): 77-82, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24964751

RESUMO

Photodynamic therapy (PDT) using photosensitized reaction to produce cytotoxicity was used for cancer therapy in recent years. To study the effectiveness of PDT mediated by a novel photosensitizer (PS), DTPP 5-(4'-(2″-dicarboxymethylamino)acetamidophenyl)-10, 15, 20-triphenylporphyrin, on lung cancer A549 cell lines in vitro, DTPP was employed in different concentrations (2, 4, 6, 8, 10, 12, 15, 20, 25, and 30 µg/ml) and combined with 650 nm laser of different power densities (0.6, 1.2, 2.4, 4.8, 7.2, and 9.6 J/cm(2)) that resulted in obvious inhibition of cell proliferation and apoptosis. Results showed that cell survival rates have a dependent relationship with time and PS concentrations and no significant cytotoxicity was induced by DTPP itself. Apoptosis and cell cycle S arrest were observed; cytoskeleton morphologic observation revealed collapse, sparkling, and shrunken shapes. Apoptosis-related protein caspase-3 overexpression was detected while caspase-9, bcl-2, and cytoskeleton protein beta-catenin were in low levels of expression than the control. Cleavage of beta-catenin by caspase-3 or other proteases from the lysosome might be the main reason for the cytoskeleton collapse as beta-tubulin and actin were at a stable level 12 h after PDT. This paper gives a better understanding of the effectiveness of DTPP-mediated PDT in lung cancer A549 cells both with regard to dosimetry and apoptosis changes.


Assuntos
Apoptose/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos da radiação , Proteínas do Citoesqueleto/metabolismo , Lasers , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/radioterapia , Compostos Organofosforados/uso terapêutico , Fármacos Fotossensibilizantes/uso terapêutico , Apoptose/efeitos da radiação , Western Blotting , Caspase 3/metabolismo , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Citoesqueleto/efeitos dos fármacos , Citoesqueleto/metabolismo , Citoesqueleto/efeitos da radiação , Humanos , Compostos Organofosforados/química , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Fase S/efeitos dos fármacos , Fase S/efeitos da radiação
7.
Cell Biochem Biophys ; 69(3): 549-54, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24477573

RESUMO

Luminal A type breast cancer was suitable for Photodynamic therapy (PDT) as its strong adhesion ability, low malignancy and easily being exposed to laser. To examine the novel photosensitizer agent 5-5-(4-N, N-diacetoxylphenyl-10, 15, 20-tetraphenylporphyrin)(DTPP) mediate PDT in breast cancer cell, Luminal A type breast cancer MCF-7 cells were used in this study, various concentrations of DTPP (0, 2, 4, 6, 8, 10, 12, 15, 20, 25, 30 µg/mL) and different time intervals (0, 0.5, 1, 2, 4, 6, 8 min) of laser exposure at 650 nm wavelength (power of 20 mW) were tested in PDT. The survival rates of MCF-7 cells were measured using a sensitive cell proliferation assay (MTT) to establish optimal semilethal dose and optimal time exposure, a further study of effects on cytoskeleton and apoptosis were also performed. Cell cycle and apoptosis variation were assayed by flow cytometry. Microtubule, microfilament, and nuclei were observed using laser scanning confocal microscopy. Oncoproteins Bcl-2, beta-tubulin, and beta-catenin were detected by means of electrophoresis. The novel DTPP showed an efficient growth inhibition of MCF-7 during PDT, effective combinations in MCF-7 cells were shown to be 4 µg mL(-1) PS irradiated for 8 min at least or 15 µg mL(-1) irradiated for 2 min at least. Microtubule, microfilament, and nucleus staining demonstrated that cytoskeletal collapse occurs at 0.5 h after PDT. Bcl-2 and skeleton adhesion proteins beta-catenin were reduced in the level of expression; whereas, skeleton proteins beta-tubulin and actin maintained similar levels of expression 12 h after PDT. These results provided a better understanding of DTPP-PDT in MCF-7 cells.


Assuntos
Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Neoplasias da Mama/patologia , Citoesqueleto/efeitos dos fármacos , Citoesqueleto/efeitos da radiação , Lasers , Porfirinas/farmacologia , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos da radiação , Proteínas do Citoesqueleto/metabolismo , Quebras de DNA de Cadeia Simples/efeitos dos fármacos , Quebras de DNA de Cadeia Simples/efeitos da radiação , Relação Dose-Resposta a Droga , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos da radiação , Humanos , Células MCF-7 , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Fatores de Tempo
8.
Bone Marrow Transplant ; 46(5): 682-9, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-20697372

RESUMO

Patients undergoing auto-SCT for neuroblastoma present a unique population to study transplant-associated thrombotic microangiopathy (TA-TMA), due to standardized chemotherapy and later exposure to radiation and cis-retinoic acid (cis-RA). We retrospectively analyzed 20 patients after auto-SCT to evaluate early clinical indicators of TA-TMA. A total of 6 patients developing TA-TMA (30% prevalence) were compared with 14 controls. Four of six patients were diagnosed with TA-TMA by 25 days after auto-SCT. Compared with controls, TA-TMA patients had higher average systolic and diastolic blood pressure levels during high-dose chemotherapy and developed hypertension by day 13 after auto-SCT. Proteinuria was a significant marker for TA-TMA, whereas blood and platelet transfusion requirements were not. Serum creatinine did not differ between groups post transplant. However, patients with TA-TMA had a 60% decrease in renal function from baseline by nuclear glomerular filtration rate, compared with a 25% decrease in those without TA-TMA (P=0.001). There was no TA-TMA-related mortality. Significant complications included end-stage renal disease (n=1) and polyserositis (n=3). Patients with TA-TMA were unable to complete cis-RA therapy after auto-SCT. We suggest that careful attention to blood pressure and urinalysis will assist in the early diagnosis of TA-TMA, whereas serum creatinine seems to be an insensitive marker for this condition.


Assuntos
Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Neuroblastoma/cirurgia , Microangiopatias Trombóticas/diagnóstico , Anti-Hipertensivos/uso terapêutico , Pressão Sanguínea , Estudos de Casos e Controles , Pré-Escolar , Feminino , Humanos , Hipertensão/tratamento farmacológico , Hipertensão/etiologia , Masculino , Proteinúria/etiologia , Estudos Retrospectivos , Microangiopatias Trombóticas/complicações , Microangiopatias Trombóticas/etiologia , Condicionamento Pré-Transplante , Transplante Autólogo
9.
Zhonghua Yi Xue Za Zhi ; 72(10): 608-11, 639-40, 1992 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-1363694

RESUMO

BALB/C mice (H-2d) receiving allogeneic C57 mice (H-2b) spleen cells via the portal vein (PV), followed by administration of cyclophosphamide (CY), abrogated the capability of rejecting allogeneic EL-4G- tumor cells (C57 origin). BALB/C mice received this combined treatment and BALB/C mice untreated or treated with either PV presensitization or CY injection were all exposed to 7.5 Gy total body irradiation, which was confirmed to be a lethal dose for BALB/C mice, then given intravenously 2 x 10(7) fetal liver cells from C57 mice (FLC57). The results indicated that the survival times of grafted BALB/C mice combined treatment were longer than those of untreated or either PV or CY treated, (PV + CY + 7.5Gy + FLC 57: MST = 99.5 +/- 12.6 day), v(N + 7.5GY + FLC57: MST = 36.5 +/- 9.8 day; PV + 7.5Gy + FLC57: MST = 12.2 +/- 0.2 day; CY + 7.5Gy + FLC57: MST = 27.6 +/- 5.5 day). The difference was statistically significant. The spleen cells from the grafted mice were assessed by indirect immunofluorescence with anti-C57 serum. The spleen cells from BALB/C mice with survival time over 100 days after grafting of FLC57 in combined treatment group were about 70-80 percentage of C57 positive cells.


Assuntos
Ciclofosfamida/farmacologia , Transplante de Tecido Fetal , Transplante de Células-Tronco Hematopoéticas , Transplante de Fígado , Imunologia de Transplantes , Animais , Feminino , Tolerância Imunológica , Fígado/embriologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Transplante de Neoplasias , Veia Porta , Transplante Homólogo
10.
Blood ; 72(4): 1168-76, 1988 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-3048436

RESUMO

We report here the results of a phase I clinical trial using counterflow centrifugal elutriation (CCE) for the removal of donor T lymphocytes before allogeneic bone marrow transplantation (BMT). Thirty-eight patients received lymphocyte-depleted allografts from HLA-identical, MLR-nonreactive sibling donors. The patients entered onto the study were either at high risk on the basis of age (median, 39 years) or disease status (acute leukemia in early relapse [ER], chronic myelogenous leukemia [CML] in accelerated phase [AP], or therapy resistant [RES] lymphoma). All patients received a standard lymphocyte dose of 1 x 10(6) morphologic lymphocytes per kilogram ideal body weight (BW) and were maintained on cyclosporine A (CsA) for 170 days after BMT. Prompt engraftment occurred in 37 of 38 patients with a median time to absolute neutrophil count (ANC) greater than 500/microL of 18 days. Although acute graft-v-host disease (GVHD; clinical stage I or greater) was observed in 45%, it was limited to the skin in all but five patients. Survival was related to disease status at the time of BMT. Among patients with acute leukemia in first or second remission, CML in chronic phase (CP) or lymphoma in partial remission (PR), 64% are currently alive, in contrast to 31% of patients with acute leukemia in third remission or early relapse, CML in second CP or AP, or RES lymphoma. Median follow-up for all patients was 351 days (range, 105 to 711 days). We conclude that this procedure is safe and warrants further evaluation in a randomized efficacy trial.


Assuntos
Transplante de Medula Óssea , Separação Celular/métodos , Centrifugação , Depleção Linfocítica , Doadores de Tecidos , Doença Aguda , Adulto , Infecções Bacterianas/etiologia , Centrifugação/métodos , Estudos de Avaliação como Assunto , Feminino , Sobrevivência de Enxerto , Doença Enxerto-Hospedeiro/etiologia , Humanos , Contagem de Leucócitos , Transfusão de Linfócitos , Masculino , Pessoa de Meia-Idade , Micoses/etiologia , Prognóstico
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