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1.
Neural Netw ; 117: 163-178, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31170576

RESUMO

With the rapid development of multimedia technology, massive unlabelled data with high dimensionality need to be processed. As a means of dimensionality reduction, unsupervised feature selection has been widely recognized as an important and challenging pre-step for many machine learning and data mining tasks. Traditional unsupervised feature selection algorithms usually assume that the data instances are identically distributed and there is no dependency between them. However, the data instances are not only associated with high dimensional features but also inherently interconnected with each other. Furthermore, the inevitable noises mixed in data could degenerate the performances of previous methods which perform feature selection in original data space. Without label information, the connection information between data instances can be exploited and could help select relevant features. In this work, we propose a robust unsupervised feature selection method which embeds the latent representation learning into feature selection. Instead of measuring the feature importances in original data space, the feature selection is carried out in the learned latent representation space which is more robust to noises. The latent representation is modelled by non-negative matrix factorization of the affinity matrix which explicitly reflects the relationships of data instances. Meanwhile, the local manifold structure of original data space is preserved by a graph based manifold regularization term in the transformed feature space. An efficient alternating algorithm is developed to optimize the proposed model. Experimental results on eight benchmark datasets demonstrate the effectiveness of the proposed method.


Assuntos
Aprendizado de Máquina não Supervisionado , Mineração de Dados/métodos
2.
Clin Lab ; 65(3)2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30868857

RESUMO

BACKGROUND: Numerous studies have shown that the hematological components of the systemic inflammatory response, including the neutrophil-to-lymphocyte ratio (NLR), the platelet-to-lymphocyte ratio (PLR), the lymphocyte-to-monocyte ratio (LMR), and the systemic immune inflammation index (SII) are efficient prognostic indicators in patients with cancers. Most of the studies did not investigate the reference intervals (RIs) of these parameters in healthy controls. METHODS: A retrospective cohort study was performed on healthy ethnic Han population aged between 18 and 79 years of age by retrieving the data from a healthy routine examination center database and laboratory infor-mation system of four participating centers in western China. By following the Clinical and Laboratory Standards Institute (CLSI), RIs of each parameter was established and validated. RESULTS: The analysis included 5,969 healthy subjects. We found that the individual's gender can significantly influence PLR, LMR, and SII (all p < 0.05), but not NLR (p > 0.05). Surprisingly, we also found that with an increase in age, the PLR, LMR, and SII tend to decrease, while NLR remained stable. PLR, LMR, and SII values were significantly higher in the young adults (18 - 64 years) than in old adults (65 - 79 years) (p < 0.001). The RIs of NLR, PLR (adults), PLR (old adults), LMR and SII were 0.88 - 4.0, 49 - 198, 42 - 187, 2.63 - 9.9, 142 x 109/L - 804 x 109/L, respectively. CONCLUSIONS: Our study addresses possible variations and establishes consensus for the NLR, PLR, LMR, and SII RIs for healthy Han Chinese adults in western China. Further, established RIs can standardize clinical applications and promote the use of these indicators into the routine complete blood count report.


Assuntos
Inflamação/sangue , Contagem de Linfócitos/estatística & dados numéricos , Adulto , Fatores Etários , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Estudos Retrospectivos , Fatores Sexuais , Adulto Jovem
3.
Cell Mol Biol (Noisy-le-grand) ; 64(6): 31-35, 2018 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-29808797

RESUMO

The Wnt/ß-catenin signaling pathway plays important roles in cancers such as colorectal cancer. Colon cancer cells secrete and express high levels of ß-catenin, which may stimulate autocrine signaling and further enhance activities of the canonical Wnt signaling pathway. Free ß-catenin in the cytoplasm and nucleus leads to its association with T cell factor (TCF)/lymphocyte enhancing factor (Lef) transcription factors, and subsequent transcriptional activation of downstream target genes. FADD plays a key role in cellular apoptosis in many different types of cancer. Therefore, a recombinant adenovirus is constructed, in which an apoptosis gene FADD is placed under control of a promoter containing Tcf-responsive elements. It is observed that FADD overexpression can suppress cell growth and enhance apoptosis of SW480 cells in vitro. In addition, Ad-FADD can also suppress the growth of subcutaneous xenografts in the nude mice. Together, these results suggest that Ad-FADD has anti-proliferative and pro-apoptotic effects in colon cancer cells, which provides a novel strategy for treatment of colorectal cancer.


Assuntos
Adenocarcinoma/terapia , Adenoviridae/genética , Neoplasias Colorretais/terapia , Proteína de Domínio de Morte Associada a Fas/fisiologia , Regulação Neoplásica da Expressão Gênica/fisiologia , Terapia Genética , Vetores Genéticos/uso terapêutico , Via de Sinalização Wnt/fisiologia , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Animais , Apoptose/fisiologia , Linhagem Celular Tumoral , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Proteína de Domínio de Morte Associada a Fas/biossíntese , Proteína de Domínio de Morte Associada a Fas/genética , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Células NIH 3T3 , Regiões Promotoras Genéticas , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Fatores de Transcrição TCF/genética , Ensaios Antitumorais Modelo de Xenoenxerto
4.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 49(2): 205-208, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29737061

RESUMO

OBJECTIVE: To study the effect of macrophage stimulating protein (MSP) on the cell cycle of non-small cell lung cancer PC14 cells without expression of recepteur d'originenanta (RON) and MSP,and analyse its effect on PC14's epithelial mesenchymal transition (EMT) capacity. METHODS: Vitro culture PC14 (blank control),PC14-Mst1-pEGFP-N1 (stablely expressed MSP) and PC14-pEGFP-N1. Cell cycles were detected by flow cytometry and the gaps between cells during growth were measured by transmission electron microscope (TEM); RT-PCR and Western blot were used to figure out the shifts of EMT related gene expression in PC14-Mst1-pEGFP-N1 cells. RESULTS: Compared with the PC14 group and PC14-pEGFP-N1 group,PC14-Mst1-pEGFP-N1 population of G1/G0 phase were significantly increased while S and G2/M phase were significantly reduced;The gaps between PC14-Mst1-pEGFP-N1 cells decreased; RT-PCR and Western blot showed that mRNA and protein levels of E-cadherin of PC14-Mst1-pEGFP-N1 were significantly higher than that of PC14,but mRNA and protein levels of Vimentin were significantly lower. CONCLUSION: MSP may affect the cell cycle of PC14 and inhibit its EMT procedure by regulating the expression of related proteins including E-cadherin and Vimentin when RON was not expressed.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Ciclo Celular , Transição Epitelial-Mesenquimal , Fator de Crescimento de Hepatócito/genética , Neoplasias Pulmonares/genética , Proteínas Proto-Oncogênicas/genética , Antígenos CD/metabolismo , Caderinas/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Humanos , Proteínas Serina-Treonina Quinases/genética , Vimentina/metabolismo
5.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 48(3): 368-372, 2017 May.
Artigo em Chinês | MEDLINE | ID: mdl-28616908

RESUMO

OBJECTIVES: To investigate the correlation between the absolute quantification of the microRNAs (miR-122, miR-451, miR-92a, miR-192) in serum during acute liver injury and the extent of liver injury on rat models of CCl4 induced acute liver injury and mice models of acetaminophen (APAP) induced acute liver injury. Furthermore, to investigate the correlation between the absolute quantification of microRNAs in serum and the drug induced liver injury pathological scoring system (DILI-PSS). METHODS: The acute liver injury model in rat by CCl4 (1.5 mL/kg), and the acute liver injury model in mice by APAP (160 mg/kg) were established. The serum at different time points on both models were collected respectively. The absolute quantification of microRNAs in serum were detected by using MiRbayTM SV miRNA Assay kit. Meanwhile, the pathological sections of liver tissue of the mice at each time point were collected to analyze the correlation between microRNAs and the degree of liver injury. RESULTS: In CCl4-induced rat acute liver injury model and APAP induced mouse acute liver injury, miR-122 and miR-192 appeared to be rising significantly, which remained the highest level at 24 h after treatment, and declined to the normal level after 72 h. In CCl4-induced rat acute liver injury model, the change of miR-92a was fluctuated and had no apparent rules, miR-451 declined gradually, but not obviously. In mice acute liver injury model induced by APAP, miR-92a and miR-451 in the progress of liver injury declined gradually, reached the lowest point at 48 h, and then recovered. The result of correlation analysis indicated that miR-122 and miR-192 presented a good positive correlation with the DILI-PSS ( r=0.741 3, P<0.05; r=0.788 3, P<0.01). CONCLUSIONS: The absolute quantification of miR-122 and miR-192 in serum has the highest level in 24 h, then decrease in 72 h, in both drug-induced and chemical liver injury. In addition, both the two microRNAs have good correlation with DILI-PSS in APAP-induced liver injury models.


Assuntos
Acetaminofen/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/sangue , Doença Hepática Induzida por Substâncias e Drogas/patologia , MicroRNAs/sangue , Animais , Biomarcadores/sangue , Fígado/patologia , Camundongos , Ratos
6.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 48(1): 41-45, 2017 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-28612556

RESUMO

OBJECTIVES: To determine the effects of macrophage stimulating protein (Msp) on the proliferation, migration and invasion of human non-small cell lung cancer cells PC14. METHODS: The eukaryotic expression vector for st1was constructed and transfected into Msp(-)and RON(-)human non-small cell lung cancer cells PC14. The expression of st1mRNA in PC14 cells was observed by RT-PCR. The expression levels of Msp protein in PC14, PC14-st1-pEGFP-N1 and PC14-pEGFP-N1 groups as well as the expression of RON in PC14 and SKBR-3 cells were detected by Western blot. RAW264.7 (mouse monocyte macrophage) and SKBR-3 cells were cultured in the supernatant of cells(PC14, PC14-st1-pEGFP-N1and PC14-pEGFP-N1 groups)and tested with Transwell microporous membrane, through which the biologic activity of Msp was evaluated by calculating the cell number migrated. The proliferation of PC14 was measured by MTT assay. The capabilities of PC14 to migrate and invade were measured by Transwell chamber and Matrigel invasion tests, respectively. RESULTS: The expressions of mRNA and protein of Mst1 in PC14 were stable after transfection with Mst1. Msp (PC14-st1 -pEGFP-N1 group) promoted the migration of RON (+) cells (SKBR-3 and RAW264.7). Compared with PC14 and PC14-pEGFP-N1 groups, the proliferation, migration and invasion of PC14 cells in PC14-st1 -pEGFP-N1 group were inhibited significantly. CONCLUSIONS: Msp can promote the migration of RON (+) cancer cells in paracrine secretion manner and inhibit the proliferation, migration and invasion of human non-small cell lung cancer cells PC14 in an unknown way.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/patologia , Movimento Celular , Proliferação de Células , Fator de Crescimento de Hepatócito/genética , Neoplasias Pulmonares/patologia , Proteínas Proto-Oncogênicas/genética , Animais , Linhagem Celular Tumoral , Humanos , Camundongos , Células RAW 264.7 , Transfecção
7.
Int J Oncol ; 47(2): 782-90, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26058363

RESUMO

Drug resistance is a major obstacle in successful systemic therapy of metastatic cancer. We analyzed the involvement of cell cycle regulatory proteins in eliciting response to N (phosphonoacetyl)-L-aspartate (PALA), an inhibitor of de novo pyrimidine synthesis, in two metastatic variants of human cancer cell line MDA-MB-435 isolated from lung (L-2) and brain (Br-1) in nude mouse, respectively. L-2 and Br-l cells markedly differed in their sensitivity to PALA. While both cell types displayed an initial S phase delay/arrest, Br-l cells proliferated but most L-2 cells underwent apoptosis. There was distinct elevation in cyclin A, and phosphorylated Rb proteins concomitant with decreased expression of bcl-2 protein in the PALA treated L-2 cells undergoing apoptosis. Markedly elevated cyclin A associated and cdk2 kinase activities together with increased E2F1-DNA binding were detected in these L-2 cells. Induced ectopic cyclin A expression sensitized Br-l cells to PALA by activating an apoptotic pathway. Our findings demonstrate that elevated expression of cyclin A and associated kinase can activate an apoptotic pathway in cells exposed to DNA antimetabolites. Abrogation of this pathway can lead to resistance against these drugs in metastatic variants of human carcinoma cells.


Assuntos
Antimetabólitos Antineoplásicos/farmacologia , Ácido Aspártico/análogos & derivados , Neoplasias Encefálicas/secundário , Ciclina A/metabolismo , Quinase 2 Dependente de Ciclina/metabolismo , Neoplasias Pulmonares/secundário , Ácido Fosfonoacéticos/análogos & derivados , Animais , Apoptose , Ácido Aspártico/farmacologia , Neoplasias Encefálicas/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/metabolismo , Camundongos , Camundongos Nus , Ácido Fosfonoacéticos/farmacologia , Regulação para Cima
8.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 42(4): 560-3, 2011 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-21866649

RESUMO

OBJECTIVE: To determine the comprehensive metabolic profiling of inflammation with capillary zone electrophoresis (CZE) in rat urine. METHODS: CZE-based metabolomics method was used to acquire urine metabolom data, with Computer Aided Similarity Evaluation System aligning peaks and analyzing urine profiling. Principle component analysis (PCA) was performed to compare and classify the phenotypes of the urine CZE spectra. RESULTS: The PCA revealed different phenotypes of metabolites between rats with and without inflammation, which were independent from gender, individual diversity, day-night diversity and diurnal variation. CONCLUSION: CZE-based metabolomics method could be used as a potential tool for urinary profiling for disease diagnosis and drug studies.


Assuntos
Inflamação/urina , Metaboloma , Metabolômica/métodos , Animais , Eletroforese Capilar , Feminino , Masculino , Análise de Componente Principal , Ratos , Ratos Wistar
9.
Am J Chin Med ; 39(2): 287-300, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21476206

RESUMO

Acute gouty arthritis is a common inflammation model with multiple pathogenic mechanisms seen in clinical practice, for which acupuncture may potentially be an alternative therapy. To investigate the effect of acupuncture on acute gouty arthritis and search for its mechanism, a metabonomic method was developed in this investigation. Acute gouty arthritis model rats were induced by monosodium urate (MSU) crystals. The urine and plasma samples were collected at several time points and the endogenous metabolites were analyzed by an ultra-performance liquid chromatography coupled with a mass spectrometry (UPLC-MS). Data were analyzed using principal components analysis (PCA) and partial least squares (PLS) analysis to compare metabolic profiles of MSU crystal-induced acute gouty arthritis rats with MSU crystal-induced acute gouty arthritis, treated with acupuncture rats. The results showed that acupuncture could restore the metabolite network that disturbed by MSU administration. Our study indicates that UPLC-MS-based metabonomics can be used as a potential tool for the investigation of biological effect of acupuncture on acute gouty arthritis.


Assuntos
Terapia por Acupuntura , Artrite Gotosa/metabolismo , Inflamação/metabolismo , Metaboloma , Metabolômica/métodos , Animais , Artrite Gotosa/sangue , Artrite Gotosa/terapia , Artrite Gotosa/urina , Cromatografia Líquida de Alta Pressão , Modelos Animais de Doenças , Inflamação/sangue , Inflamação/terapia , Inflamação/urina , Análise dos Mínimos Quadrados , Masculino , Espectrometria de Massas , Análise de Componente Principal , Ratos , Ratos Sprague-Dawley , Resultado do Tratamento , Ácido Úrico
10.
J Zhejiang Univ Sci B ; 12(1): 32-9, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21194184

RESUMO

Radiation therapy has been widely applied in cancer treatment. However, it often causes thrombocytopenia (deficiency of white blood cells) as an adverse effect. Recombinant human interleukin-6 (rhIL-6) has been found to be a very effective way against this thrombocytopenia, but IL-6 has low stability in blood, which reduces its efficacy. To increases the stability and half-life of rhIL-6, it was modified by polyethylene glycol (PEG). The pharmacokinetics and the tissue distribution of PEG-rhIL-6 labeled with (125)I were examined after subcutaneous injection in rats. The pharmacokinetic pattern of PEG-rhIL-6 was defined with linear-kinetics, and we fitted a one-compartment model with half-lives of 10.44-11.37 h (absorption, t(1/2Ka)) and 19.77-21.53 h (elimination, t(1/2Ke)), and peak concentrations at 20.51-21.96 h (t(peak)) in rats. Half-lives and t(peak) of PEG-rhIL-6 were longer than those of rhIL-6 previously reported. In the present study, for deposition of PEG-rhIL-6 in rats, the tissue distribution examination showed that blood was the major organ involved, rather than liver. However, as to the elimination of PEG-rhIL-6, the major organ was the kidney. The excretion fraction of the injection dose recovered from urine was 23.32% at 192 h after subcutaneous administration. Less than 6% of PEG-rhIL-6 was eliminated via the feces at 192 h. These results indicate that PEG-rhIL-6 is a good candidate drug formulation for patients with cancer.


Assuntos
Interleucina-6/farmacocinética , Animais , Portadores de Fármacos , Feminino , Meia-Vida , Humanos , Injeções Subcutâneas , Interleucina-6/administração & dosagem , Radioisótopos do Iodo , Masculino , Polietilenoglicóis , Estabilidade Proteica , Ratos , Ratos Wistar , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/farmacocinética , Trombocitopenia/prevenção & controle , Distribuição Tecidual
11.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 41(4): 660-3, 2010 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-20848791

RESUMO

UNLABELLED: [ OBJECTIVE: To analysis the metabonome characteristics of the urine of MSU crystal-induced acute gouty arthritis rats by the method of metabolomics. METHODS: Based on the method of metabolomics, which applies LC/MS as data acquisition platform, incorporating with the means of stoechiometry such as principal component analysis, we analyzed the metabonome difference between the urine of acute gouty arthritis rats and that of normal rats. RESULTS: Compare with control group, the metabolism status of acute gouty arthritis model group deviated. After that, with the time lapsed, it retrieved gradually to the incipient metabolism status. The variation of metabolism locus of rats measured by the methods of metabolomics properly reflects the genesis, development, and recuperation course of acute gouty arthritis. CONCLUSION: The whole metabolism status of rat model is able to be presented finely with the method of metabolomics. The metabolomics study could offer a satisfactory research approach to acute gouty arthritis.


Assuntos
Artrite Gotosa/urina , Metaboloma/fisiologia , Metabolômica/métodos , Animais , Cromatografia Líquida/métodos , Modelos Animais de Doenças , Masculino , Espectrometria de Massas/métodos , Ratos , Ratos Sprague-Dawley
12.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 27(6): 1332-5, 2010 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-21374989

RESUMO

This experimental study sought to find out the inhibitory effects of Ad-GFP-nm23-H1 on proliferation and metastasis of human colorectal carcinoma cell line Lovo, and, further, to gain an insight into some theoretical and methodical basis for instituting nm23-H1 gene therapy of cancers. MTT assay and Transwell chamber were used to detect the rates of proliferation and invasion as well as the adhesion of Lovo cells in vitro. The results demonstrated that the proliferation inhibition rates of Lovo cells treated with Ad-GFP-nm23-H1 of 10(10) PFU/ml, 10(9) PFU/ml and 10(8) PFU/ml were 84.9% +/- 1.51%, 48.5% +/- 7.23% and 22.5% +/- 5.47%, that the adherence inhibition rates of Lovo cells treated with Ad-GFP-nm23-H1 of 10(10) PFU/ml, 10(9) PFU/ml and 10(8) PFU/ml were 70.3% +/- 2.40%, 60.1% +/- 5.68% and 18.5% +/- 3.61%, and that the invasiveness inhibition rates of Lovo cells treated with Ad-GFP-nm23-H1 of 10(10) PFU/ml, 10(9) PFU/ml and 10(8) PFU/ml were 83.2% +/- 5.71%, 52.2% +/- 6.94% and 28.1% +/- 8.21%. These data suggested that Ad-GFP-nm23-H1 exerted significant inhibitory effects on the proliferation and metastasis of human colorectal carcinoma cell line Lovo in a dose-dependent way.


Assuntos
Adenoviridae/genética , Proliferação de Células , Neoplasias Colorretais/genética , Nucleosídeo NM23 Difosfato Quinases/genética , Adenoviridae/metabolismo , Linhagem Celular Tumoral , Neoplasias Colorretais/patologia , Regulação Neoplásica da Expressão Gênica , Terapia Genética , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Humanos , Nucleosídeo NM23 Difosfato Quinases/metabolismo
13.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 40(1): 149-52, 2009 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-19292067

RESUMO

OBJECTIVE: To study the pharmacokinetics and tissue distribution of PEG-rhIL-6 in rats after a single dose administration. METHODS: Pharmacokinetics and distribution of PEG-rhIL-6 in rats were studied by 125I isotope tracing method. Pharmacokinetic analysis was performed using 3P97 computer software. RESULTS: PEG-rhIL-6 declined in one-compartment model with half-lives of 10.44-11.37 h for t1/2 Ka, 19.77-21.53 h for t1/2 Ke and 20.51-21.96 h for T(pcak), respectively. PEG-rhIL-6 was mainly distributed in blood and excreted via urine. CONCLUSION: The half-lives of PEG-rhIL-6 are prolonged after being modified by PEG.


Assuntos
Interleucina-6/farmacocinética , Polietilenoglicóis/farmacocinética , Proteínas Recombinantes/farmacocinética , Animais , Feminino , Meia-Vida , Humanos , Injeções Subcutâneas , Interleucina-6/administração & dosagem , Masculino , Polietilenoglicóis/administração & dosagem , Ratos , Ratos Sprague-Dawley , Proteínas Recombinantes/administração & dosagem , Distribuição Tecidual
14.
Gene ; 429(1-2): 31-6, 2009 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-19013508

RESUMO

The complete mitochondrial genome of Macaca thibetana was determined by the long and accurate polymerase chain reaction (LA-PCR) and primer walking sequencing methods. It is 16,540 bp and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one control region. Most codon usage followed the typical pattern of vertebrates. Two rare start codons were found, in which GTG initiated the NADH dehydrogenase subunit 1 (ND1) gene and the ATP8 gene, and ATT initiated the ND2 gene. A new mitochondrial DNA-like sequence (2003 bp) in the nuclear genome of M. thibetana was found. It matched with the 3' end of the ND1 gene, the tRNA(Ile)-tRNA(Gln)-tRNA(Met) genes, the ND2 gene, and the 5' end of the tRNA(Trp) gene. Sequence divergence between the nuclear pseudogene and the mitochondrial homologue suggested that the translocation of this mtDNA fragment into the nuclear genome occurred 3.16 approximately 3.48 million years ago (MYA). Molecular phylogenetic analysis of 16 Cercopithecidae species was performed using sequences from 12 concatenated heavy-strand encoded protein coding genes. The results provided more evidence to support previous morphological and chromosomal studies on Cercopithecidae.


Assuntos
Núcleo Celular/genética , DNA Mitocondrial/genética , Genoma Mitocondrial/genética , Macaca/genética , Pseudogenes/genética , Animais , Códon/genética , Feminino , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genética , Análise de Sequência de DNA , Fatores de Tempo
15.
Mitochondrial DNA ; 19(5): 446-52, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19489138

RESUMO

Macaca thibetana is a threatened primate species endemic to China. Genetic diversities based on a 476-bp fragment at the 5'-end of the mitochondrial DNA control region HVSI were assessed. Haplotype diversity is high (0.8521), but nucleotide diversity among all haplotypes is only 0.0574. No haplotype was shared between Sichuan (SC) and Huangshan Mountain (HS) populations. Phylogenetic trees, analysis of molecular variance and network analysis consistently indicated that the SC and HS populations are significantly different. They should therefore be conserved as different units, with priority and more attention given to the HS populations.


Assuntos
DNA Mitocondrial/genética , Macaca/genética , Animais , Composição de Bases , Sequência de Bases , China , Primers do DNA/genética , DNA Mitocondrial/química , Variação Genética , Genética Populacional , Haplótipos , Região de Controle de Locus Gênico , Macaca/classificação , Dados de Sequência Molecular , Filogenia , Especificidade da Espécie
16.
Vet Immunol Immunopathol ; 114(3-4): 346-54, 2006 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-17011638

RESUMO

The cloning and sequence analysis of Tibetan macaque IFN-(gamma) and the IL-6 cDNAs are described. The Tibetan macaque IFN-gamma and IL-6 cDNAs were found to be 498 and 639 bp in length, with open reading frames encoding 165 and 212 amino acids, respectively. Homology analyses indicated that the identity levels of nucleotide and deduced amino acid sequences of IFN-gamma among primates ranged from 93.4 to 99.2%, and 87.3 to 99.4%, respectively, and that of IL-6 ranged from 92.6 to 99.8%, and 85.4 to 99.5%, respectively. Phylogenetic analysis based on amino acid sequences showed that the Tibetan macaque is most closely related to Old World monkeys, as compared to Hominoidea and New World monkeys. These findings provide insights into the evolution of primate IFN-gamma and IL-6 and additional valuable information regarding amino acid residues essential for their biological activity.


Assuntos
Interferon gama/genética , Interleucina-6/genética , Macaca/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Conservação dos Recursos Naturais , Interferon gama/imunologia , Interleucina-6/imunologia , Macaca/imunologia , Dados de Sequência Molecular , Filogenia , RNA/química , RNA/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Alinhamento de Sequência , Análise de Sequência de DNA
17.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 22(4): 798-801, 2005 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-16156276

RESUMO

The aim of this study is to evaluate the effect of Interleukin-6 on cyclophosphamide-induced hematopoietic damnification. The doses of Interleukin-6 in 3 different regimens were hypodermally injected into dogs for 7 days respectively to establish the cyclophosphamide-induced hematopoietic damnification model. The effect of Interleukin-6 on the production of platelets and the amount of other cells in the dogs' bone marrow were determined on the 21st day. The results showed that Interleukin-6 significantly alleviated the reduction of platelet count and recovered the platelets level faster. The impedance effects of Interleukin-6 directed against hematopoietic damnification of bone marrow and spleen were shown by pathological examination. These suggest that the Interleukin-6 can significantly impede cyclophosphamide-induced hematopoietic damnification.


Assuntos
Células da Medula Óssea/efeitos dos fármacos , Ciclofosfamida/efeitos adversos , Interleucina-6/farmacologia , Leucopenia/prevenção & controle , Trombocitopenia/prevenção & controle , Animais , Células da Medula Óssea/metabolismo , Cães , Feminino , Interleucina-6/uso terapêutico , Leucopenia/induzido quimicamente , Masculino , Trombocitopenia/induzido quimicamente
18.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 35(6): 806-8, 2004 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-15573759

RESUMO

OBJECTIVE: To investigate the effects of leptin on development of mouse preimplantation embryos in vitro. METHODS: (1) Groups of 2-cell stage embryos randomly selected were placed in drops of CZB medium with or without recombinant leptin (10, 50, 100 and 500 ng/ml) and were cultured to the hatched blastocyst stage, and then embryo-transfer was carried out and the implantation rate was observed and determined. (2) Groups of 2-cell stage embryos randomly selected were placed in drops of leptin free CZB medium and cultured to morula stage. Then we changed the medium, randomized the embryos into CZB medium with or without recombinant leptin (10, 50, 100 and 500 ng/ml), and cultured them to the hatched blastocyst stage. RESULTS: Addition of leptin to embryo culture media promoted the development from 2-cell stage embryo to morula, blastocyst and hatched blastocyst and improved the implantation rate. Leptin at 0, 10, 50 ng/ml concentrations caused a dose-dependent promotion, nevertheless Leptin at even higher concentrations (100 and 500 ng/ml) only brought on the same promotion as what the Leptin at concentration of 50 ng/ml did (P>0.05). Addition of leptin to embryo culture media seemed to have no effects on the in vitro development from morula to blastocyst and hatched blastocyst. CONCLUSION: Leptin plays an important role in the development of preimplantation embryo. It can promote embryo development and embryo implantation.


Assuntos
Blastocisto/metabolismo , Implantação do Embrião/efeitos dos fármacos , Leptina/farmacologia , Animais , Blastocisto/efeitos dos fármacos , Fase de Clivagem do Zigoto/metabolismo , Técnicas de Cultura Embrionária , Feminino , Leptina/genética , Leptina/metabolismo , Masculino , Camundongos
19.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 34(1): 112-4, 154, 2003 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-15600198

RESUMO

OBJECTIVE: To set up the prognostic parameters for clinical use of IVF-ET technique from the male side, the influence of the quantitative parameters about sperm motility on human in vitro fertilization rate was evaluated in this study. METHODS: The sperm samples were classfied into three groups in accordance to the eggs' in vitro fertilization rate of > or =50%, <50% and zero. Besides,based on the achievement of pregnancy after embryo transfer, the samples were divided into two groups: pregnancy achieved and pregnancy not achieved. Then we adopted the system of computer assisted sperm analysis (CASA) in assessing some parameters about sperm movement. The correlation between them and IVF rate or pregnancy was analyzed with t-test. RESULTS: This study embraced 69 IVF cycles, among which 64 cycles were completed with 20 cases of pregnancy, including biochemical and clinical pregnancies. The data on sperm movement parameters showed significant difference in Progressive motility, VAP, VSL, VCL and Percent normal sperm morphology between the > or =50% group and the <50% and zero groups (P < 0.01). Significant difference in the above parameters was also observed between the pregnancy positive and negative groups (P < 0.01). Besides, there were significant differences in Sperm movement parameters and Percent normal morphology between pre- and post-washed groups (P < 0.05). CONCLUSION: This study has demonstrated that the success rate of "test tube baby" is tightly related to some sperm movement parameters, thus suggesting VAP, VSL and VCL can serve as prognostic parameters for clinical application of IVF.


Assuntos
Fertilização In Vitro , Processamento de Imagem Assistida por Computador/métodos , Motilidade Espermática/fisiologia , Implantação do Embrião , Humanos , Masculino , Valor Preditivo dos Testes , Sêmen/citologia , Espermatozoides/citologia , Espermatozoides/fisiologia
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