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Diagn Microbiol Infect Dis ; 69(1): 7-11, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21146708

RESUMO

We evaluated the Kanagawa hemolytic test and tdh gene test for accuracy in identifying pathogenic Vibrio parahaemolyticus isolates in Shanghai. One hundred and seventy-two V. parahaemolyticus isolates were collected from diarrhea patients, freshly harvested sea fish, or fresh water samples. Statistical data for the Kanagawa hemolytic test and tdh gene test were compared. There were 83.51% isolates (81/97) from patients and 22.22% isolates (10/45) from sea-fish positive for the tdh gene. However, none of 30 isolates from fresh water samples were tdh-positive. Positive Kanagawa hemolytic tests were obtained in 88.66%, 46.67%, and 76.67% of isolates, which were from patients, sea fish, and fresh water samples, respectively. Positive rates of the Kanagawa hemolytic tests and the tdh gene tests were significantly different in isolates from those 3 sources (P < 0.001). The tdh gene test showed higher specificity than the Kanagawa hemolytic test on identifying pathogenic V. parahaemolyticus isolates in Shanghai, China.


Assuntos
Técnicas Bacteriológicas/métodos , Peixes/microbiologia , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/metabolismo , Vibrioses/diagnóstico , Vibrio parahaemolyticus/isolamento & purificação , Microbiologia da Água , Animais , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , China , DNA Bacteriano/genética , Hemólise , Humanos , Sensibilidade e Especificidade , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/patogenicidade
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