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1.
Dent Mater J ; 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34602588

RESUMO

This study aimed to evaluate the pulpal responses of monkey's pulp after direct pulp capping (DPC) with the novel mineral trioxide aggregate containing phosphorylated pullulan-based material (MTAPPL). Seventy-two teeth were randomly divided into four groups: MTAPPL; Nex-Cem MTA (NX); TheraCal LC (TH); and Dycal (DY). Histopathological changes in the pulps were observed at days 3, 7 and 70. On day 3, mild inflammatory responses were observed in the MTAPPL, no to moderate inflammatory responses in the TH, whereas moderate inflammatory responses in the NX and DY. No mineralized tissue formation (MTF) was observed in all groups. On day 7, no or mild inflammatory responses were observed in all groups. Initial MTF was observed except for DY. No inflammation with complete MTF including presence of odontoblast-like cells was observed in the MTAPPL, NX and TH groups at day 70. These findings indicate that MTAPPL could be an efficient DPC material.

2.
Materials (Basel) ; 14(8)2021 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-33918865

RESUMO

The purpose of this study was to evaluate the mechanism of action and the inhibiting effects of two types of desensitizers against dentin demineralization using pre-demineralized hypersensitivity tooth model in vitro. In this study, we confirmed that a hypersensitivity tooth model from our preliminary experiment could be prepared by immersing dentin discs in an acetic acid-based solution with pH 5.0 for three days. Dentin discs with three days of demineralization were prepared and applied by one of the desensitizers containing calcium fluoro-alumino-silicate glass (Nanoseal, NS) or fluoro-zinc-silicate glass (Caredyne Shield, CS), followed by an additional three days of demineralization. Dentin discs for three days of demineralization (de3) and six days of demineralization (de6) without the desensitizers were also prepared. The dentin discs after the experimental protocol were scanned using swept-source optical coherence tomography (SS-OCT) to image the cross-sectional (2D) view of the samples and evaluate the SS-OCT signal. The signal intensity profiles of SS-OCT from the region of interest of 300, 500, and 700 µm in depth were obtained to calculate the integrated signal intensity and signal attenuation coefficient. The morphological differences and remaining chemical elements of the dentin discs were also analyzed using scanning electron microscopy and energy-dispersive X-ray spectroscopy. SS-OCT images of CS and NS groups showed no obvious differences between the groups. However, SS-OCT signal profiles for both the CS and NS groups showed smaller attenuation coefficients and larger integrated signal intensities than those of the de6 group. Reactional deposits of the desensitizers even after the additional three days of demineralization were observed on the dentin surface in NS group, whereas remnants containing Zn were detected within the dentinal tubules in CS group. Consequently, both CS and NS groups showed inhibition effects against the additional three days of demineralization in this study. Our findings demonstrate that SS-OCT signal analysis can be used to monitor the dentin demineralization and inhibition effects of desensitizers against dentin demineralization in vitro.

3.
Sensors (Basel) ; 20(21)2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33113981

RESUMO

The aim of this study was to assess the utility of 3D imaging of optical coherence tomography (OCT) for the diagnosis of occlusal tooth wear ex vivo. Sixty-three extracted human molars with or without visible tooth wear were collected to take digital intraoral radiography and 3D OCT images. The degree of tooth wear was evaluated by 12 examiners and scored using 4-rank scale: 1-slight enamel wear; 2-distinct enamel wear; 3-tooth wear with slight dentin exposure; 4-tooth wear with distinct involvement of dentin. The degree of tooth wear was validated by the histological view of confocal laser scanning microscopy (CLSM). The sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic analysis were calculated. Diagnostic accuracy was compared with the agreement with CLSM observation using weighted kappa. The results were statistically analyzed at a significance level of α = 0.05. Three-dimensional OCT showed significantly higher sensitivity (p < 0.05) for all the diagnostic thresholds of enamel wear and dentin exposure than digital radiography (0.82, 0.85, and 0.79 vs. 0.56, 0.52, and 0.57, respectively). Three-dimensional OCT showed higher AUC and kappa coefficients than digital radiography (p < 0.05), where mean AUC and Kappa values were 0.95 and 0.76 for OCT and 0.92 and 0.47 for radiography, respectively. No significant difference of specificity was observed (p > 0.05). Three-dimensional OCT could visualize and estimate the degree of tooth wear and detect the dentin exposure at the tooth wear surface accurately and reproducibly. Consequently, a new guideline for tooth wear assessment can be proposed using OCT.


Assuntos
Tomografia de Coerência Óptica , Desgaste dos Dentes , Humanos , Imageamento Tridimensional , Curva ROC , Sensibilidade e Especificidade , Desgaste dos Dentes/diagnóstico por imagem
4.
Jpn Dent Sci Rev ; 56(1): 109-118, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33033549

RESUMO

Optical coherence tomography (OCT) is an imaging technique that can visualize the internal biological structure without X-ray exposure. Swept-source OCT (SS-OCT) is one of the latest version of OCT, wherein the light source is a tunable laser that sweeps near-infrared wavelength light to achieve real-time imaging. The imaging depth of OCT is highly influenced by the translucency of the medium. The medium that does not transmit light and the deeper structure beyond the range of light penetration depth are not relevant for OCT imaging. In OCT, sound enamel is almost transparent at the OCT wavelength range, and enamel and dentin can be distinguished from each other as the dentin-enamel junction (DEJ) appears as a dark border. Demineralized enamel and dentin are imaged as bright zones because of the formation of numerous micro-porosities where the backscatter of OCT signal is increased. In cavitated caries at interproximal or occlusal hidden zone, the upper margin of the cavity reflects the signal showing a distinct bright border in the SS-OCT image. SS-OCT is capable of determining crack penetration depth even when the cracks extended beyond the DEJ. SS-OCT has a high degree of sensitivity and specificity for the detection of dental caries and tooth cracks. SS-OCT is also capable of detecting non-carious cervical lesions and occlusal tooth wear in cross-sectional views to estimate the amount of tooth structure loss.

5.
Sci Rep ; 10(1): 15754, 2020 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-32978464

RESUMO

Optical coherence tomography (OCT) can create cross-sectional images of tooth without X-ray exposure. This study aimed to investigate the diagnostic accuracy of 3D imaging of OCT for proximal caries in posterior teeth. Thirty-six human molar teeth with 51 proximal surfaces visibly 6 intact, 16 slightly demineralized, and 29 distinct carious changes were mounted to take digital radiographs and 3D OCT images. The sensitivity, specificity and area under the receiver operating characteristic curve (AUC) for the diagnosis of enamel caries and dentin caries were calculated to quantify the diagnostic ability of 3D OCT in comparison with digital radiography. Diagnostic accuracy was evaluated by the agreement with histology using weighted Kappa. OCT showed significantly higher sensitivity, AUC and Kappa values than radiography. OCT can be a safer option for the diagnosis of proximal caries in posterior teeth that can be applied to the patients without X-ray exposure.


Assuntos
Cárie Dentária/diagnóstico por imagem , Imageamento Tridimensional , Dente Molar/diagnóstico por imagem , Tomografia de Coerência Óptica , Humanos , Sensibilidade e Especificidade
6.
Sensors (Basel) ; 20(6)2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32192069

RESUMO

Detecting the extent of occlusal caries is a clinically important but challenging task required for treatment decision making. The aim of this study was to assess the diagnostic power of 3D swept-source optical coherence tomography (OCT) for evaluation of occlusal caries in comparison with X-ray radiography. Extracted human molars not exhibiting American Dental Association (ADA) criteria advanced caries were mounted in a silicone block and digital dental radiographs were captured from the buccal side. Subsequently, occlusal surfaces were scanned with a prototype Yoshida Dental OCT. Thirteen examiners evaluated the presence and extent of caries on radiographs and dynamically sliced 3D OCT video images, using a 4 level scale-0: intact; 1: enamel demineralization without cavitation; 2: enamel caries with cavitation; 3: dentin caries with or without cavitation. Sensitivity, specificity and area under operating characteristic curves (Az) were statistically analyzed (α = 0.05). Reliability analysis showed an excellent agreement among the 13 examiners for both methods. The OCT presented a significantly higher sensitivity and Az value for the detection of caries compared to radiographs (p < 0.05). Radiography showed especially low sensitivity for dentin caries (0-2 versus 3). Dynamic slicing of 3D OCT volumes is a powerful adjunct tool to visual inspection to diagnose the dentin occlusal caries in vitro.


Assuntos
Cárie Dentária/diagnóstico , Diagnóstico Bucal/métodos , Imageamento Tridimensional/métodos , Tomografia de Coerência Óptica/métodos , Cárie Dentária/patologia , Esmalte Dentário/diagnóstico por imagem , Esmalte Dentário/patologia , Dentina/diagnóstico por imagem , Dentina/patologia , Diagnóstico Bucal/instrumentação , Humanos , Imageamento Tridimensional/instrumentação , Curva ROC , Radiografia Dentária/instrumentação , Radiografia Dentária/métodos , Distribuição Aleatória , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Tomografia de Coerência Óptica/instrumentação
7.
Restor Dent Endod ; 44(4): e45, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31799172

RESUMO

Objectives: Self-adhesive resin cements contain functional monomers that enable them to adhere to the tooth structure without a separate adhesive or etchant. One of the most stable functional monomers used for chemical bonding to calcium in hydroxyapatite is 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP). The aim of this study was to evaluate the influence of the10-MDP concentration on the bond strength and physical properties of self-adhesive resin cements. Materials and Methods: We used experimental resin cements containing 3 different concentrations of 10-MDP: 3.3 wt% (RC1), 6.6 wt% (RC2), or 9.9 wt% (RC3). The micro-tensile bond strength of each resin cement to dentin and a hybrid resin block (Estenia C&B, Kuraray Noritake Dental) was measured, and the fractured surface morphology was analyzed. Further, the flexural strength of the resin cements was measured using the three-point bending test. The water sorption and solubility of the cements following 30 days of immersion in water were measured. Results: The bond strength of RC2 was significantly higher than that of RC1. There was no significant difference between the bond strength of RC2 and that of RC3. The water sorption of RC3 was higher than that of any other cement. There were no significant differences in the three-point bending strength or water solubility among all three types of cements. Conclusions: Within the limitations of this study, it is suggested that 6.6 wt% 10-MDP showed superior properties than 3.3 wt% or 9.9 wt% 10-MDP in self-adhesive resin cement.

8.
Dent Mater J ; 38(5): 798-805, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31341147

RESUMO

The objective of this study was to determine the effect of flowable composite lining and dentin location on internal dentin fracture formation in the microtensile bond strength (MTBS) test using swept-source optical coherence tomography (SS-OCT). MTBS test beams (1.0×1.0 mm) were prepared from human superficial and deep dentin, which was bonded with a self-etch adhesive (Clearfil SE Bond) and hybrid composite resin (Clearfil AP-X), with or without flowable lining (Clearfil Majesty ES-Flow). We tested 4 groups according to placement technique (with vs. without flowable liner) and dentin (superficial vs. deep) locations. Cross-sectional 2D images of the bonded interface were obtained before and after the MTBS test. Internal dentin fracture after MTBT was observed as a bright zone in SS-OCT. Flowable lining significantly reduced internal fracture formation in dentin (p<0.05). Dentin location significantly influenced MTBS (p<0.05), and this was reduced by flowable lining usage.


Assuntos
Colagem Dentária , Adesivos Dentinários , Resinas Compostas , Estudos Transversais , Análise do Estresse Dentário , Dentina , Humanos , Teste de Materiais , Cimentos de Resina , Propriedades de Superfície , Resistência à Tração
9.
Dent Mater J ; 38(5): 779-789, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31341148

RESUMO

Evaluation of gap formation at the interfaces of a two-step self-etching adhesive with/without pre-etching was performed using sweptsource optical coherence tomography (SS-OCT). Round cavities were prepared in bovine incisors at the middle (MC) and cervical (CC) thirds of the crown and the cervical third of the root (CR). Clearfil SE bond was directly applied to one group (SE) and another (PA) was pretreated with K-etchant gel. Following restoration by flowable composite resin, the teeth were thermally challenged and stored for 2 months. Interfacial gaps observed in the cross-sectional OCT images were analyzed and the bottom cavities exhibited increased gaps compared to the margin and dentin-enamel junction (DEJ). The CR site had a larger gap than at MC and CC in the SE group. DEJ separation at the MC was significantly smaller than that at CC in both groups. Therefore, gap formation depends on the cavity region, location, and bonding protocol.


Assuntos
Colagem Dentária , Adesivos Dentinários , Animais , Bovinos , Resinas Compostas , Estudos Transversais , Coroas , Preparo da Cavidade Dentária , Esmalte Dentário , Restauração Dentária Permanente , Dentina , Cimentos de Resina , Tomografia de Coerência Óptica
10.
Dent Mater ; 35(4): e74-e82, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30770133

RESUMO

OBJECTIVE: The purpose of this study was to detect and investigate the association of enamel microcracks with demineralization at proximal contact areas of premolars, using 3D swept-source optical coherence tomography (SS-OCT). METHODS: Extracted maxillary and mandibular premolars (n=50 each), without any visible tooth cracks, were examined for demineralization of interproximal contact areas, using the International Caries Detection and Assessment System (ICDAS). SS-OCT was used to evaluate demineralization and detect microcracks. Demineralization progression was divided into 4 types by depth: 0 for sound enamel and Type I, II, and III for enamel demineralization penetrating into the outer third, the middle third, and the inner third of the enamel thickness, respectively. Enamel microcracks were classified according to the predominant crack pattern: Type O, no cracks; Type A, subsurface microdefects; Type B, enamel microcrack running along the direction of the enamel prism; Type C, enamel microcrack running transverse to the enamel prism direction; and Type D, a combination of patterns A, B, and C. SS-OCT findings were confirmed by confocal laser scanning microscopy observation. RESULTS: Microcracks confined within the superficial enamel in proximal contact areas were seen as bright lines on SS-OCT. There were significant positive correlations among ICDAS codes, demineralization levels, and microcrack distribution (p≤0.001). The mesial side of maxillary premolars showed significantly more demineralization (n=36) and microcracks (n=27) than that of the mandibular premolars (n=20 and n=14, respectively; p<0.001). SIGNIFICANCE: The presence of microcracks within the superficial enamel in proximal contact areas could be determined using SS-OCT, and correlated with the level of demineralization. 3D SS-OCT is a valuable diagnostic tool for comprehensive assessment of microstructural changes related to enamel demineralization and crack development.


Assuntos
Cárie Dentária , Tomografia de Coerência Óptica , Esmalte Dentário , Humanos , Microscopia Confocal
11.
Lasers Med Sci ; 34(5): 981-989, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30448940

RESUMO

The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD490) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD490 measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.


Assuntos
Cárie Dentária/microbiologia , Streptococcus mutans/efeitos da radiação , Streptococcus sobrinus/efeitos da radiação , Raios Ultravioleta , Contagem de Colônia Microbiana , Dentina/efeitos da radiação , Humanos , Viabilidade Microbiana/efeitos da radiação , Streptococcus mutans/crescimento & desenvolvimento , Streptococcus sobrinus/crescimento & desenvolvimento
12.
Dent Mater J ; 37(6): 880-888, 2018 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-29962412

RESUMO

The purpose of this study was to compare optical properties of root caries under two observing conditions using swept-source optical coherence tomography (SS-OCT). In vitro and natural root caries were observed by SS-OCT under wet and dry conditions, followed by confocal laser scanning microscope (CLSM) and transverse microradiography (TMR). Signal intensity (SI), distance between SI peaks (SI-distance) and optical lesion depth were obtained from OCT. Lesion depth was measured from CLSM; lesion depth (LDTMR) and mineral loss (ML) were obtained from TMR. In vitro root caries under wet and dry conditions showed different OCT images and SI patterns. Lesion depth of OCT and that of CLSM, SI-distance and LDTMR, LDTMR and ML significantly correlated. Under dry conditions, half natural root caries showed similar OCT images and SI patterns as in vitro root caries. The base of demineralized dentin could be detected more clearly under dry conditions than under wet conditions.


Assuntos
Microrradiografia/métodos , Microscopia Confocal/métodos , Cárie Radicular/diagnóstico por imagem , Tomografia de Coerência Óptica/métodos , Humanos , Técnicas In Vitro , Desmineralização do Dente/diagnóstico por imagem
13.
Dent Mater J ; 37(5): 754-760, 2018 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-29848855

RESUMO

This study determined the influence of thermocycling (TC) and flowable composite on microtensile bond strength (MTBS), crack formation and mechanical properties of the bonding interface using swept-source optical coherence tomography (SS-OCT) and nanoindentation. MTBS test beams prepared from human dentin bonded with self-etch adhesive and hybrid composite with or without flowable lining were aged for either 0 or 10,000 thermocycles, resulting in 4 groups of specimens according to the placement technique and TC (n=10). 2D images were obtained before and after MTBS test to detect crack at interface using SS-OCT. Hardness across resin-dentin bonding area were measured using nanoindentation. Two-way ANOVA showed that flowable lining significantly increased MTBS (p<0.05). TC significantly increased crack percentage in composite while there was no significant difference in dentin crack. Moreover, TC significantly affected the hardness of dentin and resin composites (p<0.05). SS-OCT is effective in detecting internal fracture in substrate.


Assuntos
Resinas Compostas/química , Adesivos Dentinários/química , Condicionamento Ácido do Dente , Colagem Dentária , Análise do Estresse Dentário , Testes de Dureza , Humanos , Técnicas In Vitro , Teste de Materiais , Propriedades de Superfície , Resistência à Tração , Tomografia de Coerência Óptica
14.
Dent Mater ; 30(11): 1213-23, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25199439

RESUMO

OBJECTIVE: Adding antimicrobial/anti-MMP quaternary ammonium methacrylates (QAMs) to comonomer blends should not weaken the mechanical properties of dental resins. This work evaluated the degree conversion and mechanical properties of BisGMA/TEGDMA/HEMA (60:30:10) containing 0-15 mass% QAMs A-E (A: 2-acryloxyethyltrimethyl ammonium chloride; B: [3-(methacryloylamino)propyl]trimethylammonium chloride; C: [2-(methacryloxy)ethyl] trimethyl ammonium chloride; D: diallyldimethyl ammonium chloride; E: 2-(methacryloyloxy) ethyltrimethyl ammonium methyl sulfate. METHODS: Unfilled resins with and without QAM were placed on ATR-FTIR and light-polymerized for 20s in a thin film at 30°C. Unfilled resin beams were casted from square hollow glass tubings. Half of the beams were tested after 3 days of drying (control); the other half were tested wet after 3 days of water storage. RESULTS: Addition of QAMs in control resins significantly increased conversion 600 s after light termination, with the exception of 5% MAPTAC (p<0.05). Increase of QAM content within a formulation significantly increased conversion. Control beams gave dry Young's moduli of ∼700 MPa. Addition of 5, 10 or 15 mass% QAMs produced significant reductions in dry Young's moduli except for 5% B or C. 15 mass% A, B and C lowered the wet Young's moduli of the resin beams by more than 30%. The ultimate tensile stress (UTS) of control dry resin was 89±11 MPa. Addition of 5-10 mass% QAMs had no adverse effect on the dry UTS. After water storage, the UTS of all resin blends fell significantly (p<0.05), especially when 15 wt% QAMs was added. Control dry beams gave fracture toughness (KIC) values of 0.88±0.1 MPa m(1/2). Wet values were significantly higher at 1.02±0.06 (p<0.05). KIC of dry beams varied from 0.85±0.08 at 5% QAMs to 0.49±0.05 at 15% QAMs. Wet beams gave KIC values of 1.02±0.06 MPa m(1/2) that fell to 0.23±0.01 at 15% QAMs. SIGNIFICANCE: Addition of 10% QAMs increased the degree of conversion of unfilled resins, but lowered wet toughness and UTS; addition of 15% QAMs lowered the mechanical properties of wet resins below acceptable levels.


Assuntos
Resinas Compostas , Teste de Materiais , Metacrilatos/química , Compostos de Amônio Quaternário/química , Espectroscopia de Infravermelho com Transformada de Fourier
15.
Dent Mater J ; 32(3): 420-4, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23719003

RESUMO

It is known that chlorhexidine (CHX) does not inhibit micro-tensile bond strengths (MTBS) when it is used in etch-and-rinse adhesives. In that technique, CHX is applied to dentin as a primer after phosphoric acid-etching before bonding with Single Bond. It would be more convenient if it is possible to incorporate CHX into the adhesive. The purpose of this study was to compare the MTBS and the FT-IR percent conversion of an all-in-one self-etching adhesives contained varying concentration of CHX. Extracted human third molars were bonded with a control all-in-one adhesive or experimental versions containing 0.5, 1, 2 or 5% CHX. The MTBS and the percent conversion of experimental adhesives containing up to 1% CHX were not significantly CHX-free control adhesives. However, addition of 2 or especially 5% CHX experimental adhesives produced significant reductions in both the MTBS and the percent conversion.


Assuntos
Anti-Infecciosos Locais/farmacologia , Clorexidina/farmacologia , Colagem Dentária/métodos , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Cimentos de Resina/química , Análise de Variância , Corrosão Dentária/métodos , Falha de Restauração Dentária , Análise do Estresse Dentário , Dentina/ultraestrutura , Humanos , Teste de Materiais , Polimerização , Espectroscopia de Infravermelho com Transformada de Fourier , Estatísticas não Paramétricas , Resistência à Tração
16.
Int J Dent ; 2012: 278623, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22606202

RESUMO

The purpose of this paper was to compare the bond strengths of the self-adhesive luting cements between ceramics and resin cores and examine their relation to the cement thickness. Three self-adhesive luting cements (Smartcem, Maxcem, and G-CEM) and a resin cement (Panavia F 2.0) for control were used in the paper. The thickness of the cements was controlled in approximately 25, 50, 100, or 200 µm. Each 10 specimens were made according to the manufacturers' instructions and stored in water at 37°C. After 24 hours, microtensile bond strength (µTBS) was measured. There were significant differences in cements. Three self-adhesive cements showed significantly lower µTBSs than control that required both etching and priming before cementation (Tukey, P < 0.05). The cement thickness of 50 or 100 µm tended to induce the highest µTBSs for each self-adhesive luting cements though no difference was found.

17.
J Adhes Dent ; 14(2): 129-36, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21935518

RESUMO

PURPOSE: The use of sodium hypochlorite (NaOCl) can decrease the bond strength of 4-META/MMA-TBB resin cement to root dentin. The aim of this in vitro study was to evaluate the effect of sodium toluene sulfinic acid (SA) as a pretreatment to increase bond strength to NaOCl-treated dentin. MATERIALS AND METHODS: The root canal dentin of bovine teeth was treated with 6 methods: group 10-3 (10% citric acid/3% ferric chloride; control); group SA; group NC (sodium hypochlorite/10-3 solution); group NS-10-3 (NaOCl + sulfinic acid + 10-3 solution); group HSA-10-3 (NaOCl +H2O2 +NaOCl + sulfinic acid + 10-3 solution); group HO-10-3 (NaOCl + H2O2 + NaOCl +10-3 solution). The roots were then filled with Super-Bond C&B (SB) or Super-Bond sealer (SBS). Samples were stored in distilled water for 24 h at 37°C and then cross sectioned into five slabs (0.8 mm thick) that were subjected to "trimming" microtensile bond strength testing. All treated dentin surfaces and resin/dentin bond interfaces were analyzed under SEM. RESULTS: Compared with the control, NaOCl treatment significantly decreased the resin/dentin bond strengths. However, SA treatment following NaOCl irrigation resulted in no significant differences of bond strength values. CONCLUSION: Sulfinic acid was effective in restoring 4-META/MMA-TBB resin cement bond strength after NaOCl irrigation.


Assuntos
Colagem Dentária/métodos , Adesivos Dentinários , Dentina/efeitos dos fármacos , Cimentos de Resina , Tolueno/análogos & derivados , Animais , Compostos de Boro , Bovinos , Cavidade Pulpar , Análise do Estresse Dentário , Dentina/ultraestrutura , Permeabilidade da Dentina/efeitos dos fármacos , Metacrilatos , Metilmetacrilatos , Microscopia Eletrônica de Varredura , Irrigantes do Canal Radicular/farmacologia , Hipoclorito de Sódio/farmacologia , Resistência à Tração , Tolueno/farmacologia
18.
Dent Mater J ; 29(4): 362-8, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20610877

RESUMO

The aim of this study was to evaluate the fluoride release, neutralizing ability and inhibitory effect on secondary caries of resin-based materials containing a silane-coated glass filler. Resin-based materials containing fluoro-boro-alumino-silicate glass coated by 3-methacryloxypropyltrimethoxysilane or 3-aminopropyltrimethoxysilane were used and resin material containing glass filler without coating was used as a control. The fluoride release and pH value after immersion were measured for 10 weeks. The inhibitory effect was also evaluated. During the initial period, the material with 3-aminopropyltrimethoxysilane gave the greater amount of fluoride release and produced a higher pH value compared with the other materials. However, the neutralizing ability of the material with 3-aminopropyltrimethoxysilane filler became weaker with ageing of the specimens. The mean depth of outer lesions was similar among the three materials. Within the limitations of this study, secondary caries around restorations could not be inhibited even for products showing high fluoride releasing and neutralizing ability.


Assuntos
Cariostáticos/química , Resinas Compostas/química , Materiais Dentários/química , Fluoretos/química , Vidro/química , Silanos/química , Silicatos de Alumínio/química , Animais , Bis-Fenol A-Glicidil Metacrilato/química , Bovinos , Cárie Dentária/prevenção & controle , Restauração Dentária Permanente , Umidade , Concentração de Íons de Hidrogênio , Imersão , Teste de Materiais , Metacrilatos/química , Polietilenoglicóis/química , Ácidos Polimetacrílicos/química , Propilaminas/química , Distribuição Aleatória , Cimentos de Resina/química , Temperatura , Fatores de Tempo
19.
Dent Mater J ; 28(4): 471-6, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19721285

RESUMO

The aim of this study was to investigate the effect of Er:YAG laser irradiation on human dentin surface using X-ray photoelectron spectroscopy (XPS). 10 human dentin disks were prepared from extracted human molars for XPS analysis. These specimens were divided into two groups of five: a control group and group that were irradiated by an Er:YAG laser beam (100 mJ, 1Hz). All specimens were analyzed by XPS over a wide scanning range and narrow scanning ranges. The Ca/P ratio was calculated from the XPS results. In the results, the binding energies of Ca, P, and N in the laser-irradiated group were higher than those in the control group. The Ca/P ratio of the Er:YAG laser irradiated group (1.24+/-0.05) was significantly lower than that of the control group (1.52+/-0.16). This study showed that Er:YAG laser irradiation decreased Ca/P ratio and denatured the collagen of human dentin.


Assuntos
Colagem Dentária , Dentina/efeitos da radiação , Lasers de Estado Sólido/efeitos adversos , Cálcio/análise , Cálcio/química , Colágeno/química , Colágeno/efeitos da radiação , Dentina/química , Humanos , Nitrogênio/análise , Fósforo/análise , Espectroscopia Fotoeletrônica , Desnaturação Proteica , Propriedades de Superfície
20.
Dent Mater J ; 26(4): 481-6, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-17886450

RESUMO

This study was designed to examine the effects of dose of recombinant human BMP-2 (rhBMP-2) on bone formation at palatal sites in 10-week-old (10w) and 70-week-old (70w) rats, when combined with a polylactate-polyglycolate copolymer/gelatine sponge (PGS). New bone formation was observed at six weeks after implantation. In the 10w rats, thickness of new bone (TNB) increased as the dosage increased from 0 microg to 4 microg, and decreased significantly as the dosage increased from 8 microg to 24 microg. In contrast, in the 70w rats, TNB increased as the dosage increased from 0 microg to 16 microg, and did not significantly change as the dosage increased from 16 microg to 24 microg. These results suggest that the most effective dosage of rhBMP-2 for induction of bone formation varies according to age.


Assuntos
Proteínas Morfogenéticas Ósseas/administração & dosagem , Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos/administração & dosagem , Palato/efeitos dos fármacos , Fator de Crescimento Transformador beta/administração & dosagem , Fatores Etários , Animais , Proteína Morfogenética Óssea 2 , Relação Dose-Resposta a Droga , Humanos , Masculino , Palato/cirurgia , Ratos , Ratos Wistar
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