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1.
Thorac Cancer ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32012484

RESUMO

BACKGROUND: There is no consensus on the definition or recommended radiotherapy treatment of ultracentral non-small cell lung cancer (NSCLC). Here, we report our institution's experience in treating ultracentral lung cancer patients with stereotactic ablative radiotherapy (SABR) of 60 Gy in eight fractions. METHODS: We retrospectively reviewed the outcomes of 21 ultracentral NSCLC patients treated with 60 Gy SABR in eight fractions. We defined ultracentral lung cancer as the planning target volume (PTV) directly abutting or overlapping central structures, including the proximal bronchial tree, heart, and great vessels but not the esophagus. The Kaplan-Meier method was used to estimate overall survival (OS), progression-free survival (PFS) and local control (LC). Toxicity was scored per the CTCAE v4.03. RESULTS: The median follow-up time was 15 months, and the median OS was 15 months. The one- and two-year OS rates were 87.5% and 76.6%, respectively. The one- and two-year PFS rates were 71.1% and 64.0%, respectively. The one- and two-year LC rates were 92.9% and 92.9%, respectively. The rate of grade 2 treatment-related toxicities was 19.1%. There was no grade ≥ 3 treatment-related toxicity. CONCLUSION: SABR of 60 Gy in eight fractions is feasible for ultracentral NSCLC.

2.
Chemosphere ; 244: 125541, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32050339

RESUMO

Chironomus dilutus is a prominent model species in conventional sediment toxicity testing and sediment contamination diagnosis. However, lack of genomic data significantly limited its application in identifying toxicological mode of action (MOA) and molecular biomarkers of toxicants. Here the transcriptome of C. dilutus in full life span and both sexes (1st, 2nd, 3rd and 4th instar larvae, pupae, and adults) were developed and temporal gene expression across adjacent life stages were investigated to understand the regulation of development. Furthermore, transcriptional response of Midges (the 4th instar larvae) exposed to chemicals of different MOAs (CdCl2, nonylphenol and triclosan) were profiled based on the reference transcriptome. Consequently, a complete transcriptome of 31132 unigenes with N50 of 3117bp, covering 98.8% of the arthropod single-copy orthologs were assembled. While 364 genes were differentially expressed among adjacent larval stages, 7142 and 2127 of transcripts were significantly changed for the transition of larvae-pupae and pupae-adults, respectively. Finally, chemical-specific gene expression profile were identified in the midges, showed its potential in classifying distinct contaminants. Overall, the comprehensive transcriptome of C. dilutus developed here could not only facilitate the mechanistic understanding of environmental toxicants during critical life stage of aquatic insects, but also provide molecular diagnostic tools in sediment ecotoxicology.

3.
Sci Total Environ ; 714: 136878, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-32018995

RESUMO

Neonicotinoid insecticides have been frequently detected in surface water due to extensive use worldwide, however, little information is available for the regional characteristics and ecological risk of neonicotinoids in sediment. In the current study, six neonicotinoids were analyzed in 58 sediment samples from agricultural (vegetable and rice planting) and urban areas in South China. Neonicotinoids were ubiquitous in the sediments, with maximum, mean and median concentrations of 23.8, 4.21 and 2.73 ng·g-1 dry weight, respectively. Neonicotinoids were detected more often and at higher concentrations in vegetable planting and urban areas while clothianidin and imidacloprid dominated neonicotinoid composition in the rice-planting area. Multiple correspondence analysis showed the distribution of sediment-bound neonicotinoids were significantly affected by crop type, distance to the source, and physicochemical properties of neonicotinoids. While more hydrophilic neonicotinoids tended to migrate to the streams, those with log Kow > 0 are usually retained in the ditches near the treated fields. Neonicotinoids with shorter half-lives (acetamiprid and thiacloprid) were detected more frequently in vegetable planting areas, yet more persistent imidacloprid and clothianidin were more likely to be detected in rice planting areas. It was in accordance with application patterns of neonicotinoids in different crops. Environmental exposure distributions indicated that sediment-bound neonicotinoids, except for thiacloprid, posed considerable risk to aquatic invertebrates, which are important for ecological functioning of aquatic ecosystems, calling for better measures to control and manage of neonicotinoid risks.

4.
Environ Pollut ; 260: 114011, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31991362

RESUMO

Neonicotinoid insecticides have posed a great threat to non-target organisms, yet the mechanisms underlying their toxicity are not well characterized. Major modes of action (MoAs) of imidacloprid were analyzed in an aquatic insect Chironomus dilutus. Lethal and sublethal outcomes were assessed in the midges after 96-h exposure to imidacloprid. Global transcriptomic profiles were determined using de novo RNA-sequencing to more holistically identify toxicity pathways. Transcriptional 10% biological potency values derived from ranked KEGG pathways and GO terms were 0.02 (0.01-0.08) (mean (95% confidence interval) and 0.05 (0.04-0.06) µg L-1, respectively, which were more sensitive than those from phenotypic traits (10% lethal concentration: 0.44 (0.23-0.79) µg L-1; 10% burrowing behavior concentration: 0.30 (0.22-0.43) µg L-1). Major MoAs of imidacloprid in aquatic species were identified as follows: the activation of nicotinic acetylcholine receptors (nAChRs) induced by imidacloprid impaired organisms' nerve system through calcium ion homeostasis imbalance and mitochondrial dysfunction, which posed oxidative stress and DNA damage and eventually caused death of organisms. The current investigation highlighted that imidacloprid affected C. dilutus at environmentally relevant concentrations, and elucidated toxicity pathways derived from gene alteration to individual outcomes, calling for more attention to toxicity of neonicotinoids to aquatic organisms.

5.
Medicine (Baltimore) ; 99(4): e18543, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31977847

RESUMO

Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer with a high mortality disease which has been positioned the first and second cancer morbidity of men and women in China, separately. Our study was to assess the prognostic meaningful of ubiquitin conjugating enzyme E2 T (UBE2T) expression in LUAD dependent on data acquired from The Cancer Genome Atlas (TCGA) and so as to increase further knowledge into the biological pathways involved in LUAD pathogenesis related to UBE2T.Information on gene expression and comparing clinical data were recognized and downloaded from TCGA. Gene set enrichment analysis (GSEA) created an arranged list of all genes s indicated by their connection with UBE2T expression.Our study cohort included 265 (54.5%) female and 221 (36.0%) male patients. The scatter plot and paired plot showed the difference of UBE2T expression between normal and tumor samples (P < .01). Overall survival (OS) analysis demonstrated that LUAD with UBE2T-high had a more terrible prognosis than that with UBE2T-low (P < .01). Multivariate analysis with the cox proportional hazards model indicated that the expression of UBE2T (hazard ratio [HR]: 1.28; 95% Confidence Interval (CI): 1.06-1.56; P = .011) and stage (HR: 2.02; 95% CI: 1.27-3.21; P = .003) were independent prognostic factors for patients with LUAD. The GSEA results showed that cell cycle, DNA replication, RNA degradation, oxidative phosphorylation, pathogenic Escherichia coli infection, citrate cycle tricarboxylic acid cycle, Alzheimer's disease, P53 signaling pathway, and purine metabolism are differentially enriched in UBE2T high expression phenotype.Our study found that the expression of UBE2T was significantly increased in LUAD patients and associated with several clinical features. UBE2T may be a potentially useful prognostic molecular biomarker of bad survival in LUAD, while further experimental ought to be performed to demonstrate the biologic effect of UBE2T.


Assuntos
Adenocarcinoma de Pulmão/patologia , Neoplasias Pulmonares/patologia , RNA Mensageiro/biossíntese , Enzimas de Conjugação de Ubiquitina/biossíntese , Adenocarcinoma de Pulmão/mortalidade , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Ciclo Celular , Feminino , Humanos , Neoplasias Pulmonares/mortalidade , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Fenótipo , Prognóstico , Fatores de Risco , Fatores Sexuais , Análise de Sobrevida
6.
Environ Sci Technol ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31809028

RESUMO

Research has already demonstrated the toxic effects of microplastics (MPs) on different biota. However, the underlying toxic mechanism of MPs remains to be elucidated, especially the effect of particle size and the presence of dissolved organic matter in water. This study investigated the impact on Scenedesmus obliquus exposed in five types of polystyrene particle suspensions with different sizes and surface charges, in the presence and absence of humic acid (HA). Results indicated that the 50% growth inhibition rate of S. obliquus showed no significant difference between the five types of MPs, but the toxic mechanism varied with particle size. Larger size MPs caused adverse effects by blocking the light transport and affecting photosynthesis, while smaller ones destroyed the cell wall by adsorbing onto the algae surface. Also, the addition of HA significantly alleviated the toxicity of smaller size MPs, but not of the larger ones. Scanning electron microscopy images and the reactive oxygen species assay demonstrated that the HA could form a corona on the surface of MPs, reduce the affinity to microalgae, and minimize the adverse effect. Together, these findings identified important factors in determining the toxicity of MPs, providing valuable data for risk assessment of MPs.

7.
BMC Psychiatry ; 19(1): 386, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31805901

RESUMO

BACKGROUND: China has experienced rapid socioeconomic, and health transitions over the last four decades, and urban-rural disparities are becoming increasingly apparent. Research on depression among rural and urban students can provide evidence on the relationship between sociodemographic characteristics and adolescent depression. METHODS: We examined the association between sociodemographic characteristics and adolescent depression among 3605 students from Wuhan city and Jianli county that was recruited from the local junior middle school via a cross-sectional study. Univariate and multivariate logistic regression models were used to explore the sociodemographic characteristics of adolescent depression in urban and rural areas, respectively. Nomograms were constructed to calculate individual depression risk of junior middle school students. RESULTS: 32.47% of rural students and 35.11% of urban students display depressive symptoms. The protective factors of depression in urban students are exercise habit, younger, key class, better academic achievement and males, while Left-behind children (LBC), poor academic achievement and females had higher depression risk in rural area. Two nomograms were constructed to screen the adolescent depression in urban and rural junior middle school students, respectively. The clinical tools were well calibrated. CONCLUSION: The field-based research examined sociodemographic characteristics potentially associated with adolescent depression and offered an effective and convenient tool of individualized depression risk evaluation for junior middle school students. Future longitudinal epidemiologic research on adolescent depression may help to further validate the discovery of present study, which will support developing policies and practices to minimize the factors of adolescent depression.

8.
J Hazard Mater ; : 121853, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31874758

RESUMO

Chemical mixtures are a common occurrence in contaminated sediment and determining causal relationship between sediment contamination and adverse outcomes is challenging. The bioavailability and choice of bioassay endpoints played important roles in elucidating causality. As such, bioaccessibility-based XAD extraction and adverse outcome pathway (AOP) guided bioassays were incorporated into an effect-directed analysis to more effectively determine sediment causality. XAD extracts of sediments from urban waterways in Guangzhou, China were examined using cell viability bioassays with four human tumor cells from lung, liver, breast, and bone marrow. Pronounced effects to SH-SY5Y cells were noted, thus neurotoxicity was subsequently focused in the AOP-guided bioassays. Intracellular calcium influx, mitochondrial membrane potential inhibition, reactive oxygen species generation, and cell viability were utilized as evidence for neurotoxicity AOP-guided analysis. Suspect toxicants were identified in active fractions using GC-MS. Toxicity confirmation was performed by evaluating toxicity contributions of the candidates to the pathway. Cypermethrin, bisphenol A, galaxolide, tonalide, and versalide were found as the major stressors across key events of the studied pathway. Moreover, good correlations among key events validated the feasibility of method to predict in vivo response, suggesting that considering bioavailability and AOP improved environmental relevance for toxicant identification in a complex mixture.

9.
Zhongguo Zhong Yao Za Zhi ; 44(18): 3954-3959, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31872730

RESUMO

Through investigation,it was found that the main disease of leaves was grey mold on Dendrobium officinale in Hubei province,which has a great impact on the yield and quality of D. officinale. The identification of morphological and molecular biological was used to prove that the pathogen was Botrytis cinerea. Through test the effect of 5 plant source fungicides and 4 antibiotic fungicides on mycelial growth of strain HS1,which proved 0. 3% eugenol had the best inhibitory effect,EC50 was 0. 29 mg·L-1,the second was1% osthol and EC50 was 1. 12 mg·L-1,the EC50 of 0. 5% matrine was 9. 16 mg·L-1,the EC50 of the other six fungicides was higher than 10 mg·L-1. The field control effect test proved that 0. 3% eugenol had the best control effect,reaching 89. 44%,secondly for 1%osthole,which was 77. 17%,0. 5% matrine was in the third place with 62. 37% of effective rate. However,the control effect of the other fungicides was less than 60%. The three plant-derived fungicides were safe for the produce of D. officinale and showed no phytotoxicity. The effect of these fungicides on the growth of D. candidum was tested,and proved that all the fungicides were safe and harmless to D. candidum. This study provides a research basis for the safe and effective prevention and control gray mold of D. officinale.


Assuntos
Botrytis/patogenicidade , Dendrobium/microbiologia , Fungicidas Industriais , Doenças das Plantas/microbiologia , Alcaloides , Botrytis/efeitos dos fármacos , Cumarínicos , Eugenol , Doenças das Plantas/prevenção & controle , Folhas de Planta/microbiologia , Quinolizinas
10.
Sci Rep ; 9(1): 20116, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31882918

RESUMO

In recent experiments, superconductivity and correlated insulating states were observed in twisted bilayer graphene (TBG) with small magic angles, which highlights the importance of the flat bands near Fermi energy. However, the moiré pattern of TBG consists of more than ten thousand carbon atoms that is not easy to handle with conventional methods. By density functional theory calculations, we obtain a flat band at EF in a novel carbon monolayer coined as cyclicgraphdiyne with the unit cell of eighteen atoms. By doping holes into cyclicgraphdiyne to make the flat band partially occupied, we find that cyclicgraphdiyne with 1/8, 1/4, 3/8 and 1/2 hole doping concentration shows ferromagnetism (half-metal) while the case without doping is nonmagnetic, indicating a hole-induced nonmagnetic-ferromagnetic transition. The calculated conductivity of cyclicgraphdiyne with 1/8, 1/4 and 3/8 hole doping concentration is much higher than that without doping or with 1/2 hole doping. These results make cyclicgraphdiyne really attractive. By studying several carbon monolayers, we find that a perfect flat band may occur in the lattices with both separated or corner-connected triangular motifs with only including nearest-neighboring hopping of electrons, and the dispersion of flat band can be tuned by next-nearest-neighboring hopping. Our results shed insightful light on the formation of flat band in TBG. The present study also poses an alternative way to manipulate magnetism through doping flat band in carbon materials.

11.
Huan Jing Ke Xue ; 40(6): 2858-2868, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854680

RESUMO

Understanding the effects of different levels of nitrogen fertilizer applications on soil respiration rates and soil biochemical properties is of great importance for providing a theoretical basis for accurate assessments of the soil respiration intensity and carbon recycling in grassland ecosystems. A field experiment was performed from April 2017 to March 2018, in which four different levels of nitrogen applications were investigated, including 0 kg·hm-2 (N0), 60 kg·hm-2 (N1), 120 kg·hm-2 (N2), and 180 kg·hm-2 (N3). The seasonal changes in the soil respiration rate, soil temperature, and soil moisture in the alfalfa grassland under different levels of nitrogen applications were observed, and soil biochemical characteristics were observed after each harvest in the growing season. The results showed that soil respiration rate of the alfalfa grassland displayed significant seasonal variation under different nitrogen levels. In particular, the soil respiration rate reached a peak during the last 10-day period of July and then decreased to the minimum in mid-December. During the growing season of alfalfa, the soil respiration rate of the alfalfa grassland increased with the increases in the nitrogen application rate. The mean soil respiration rates of the N1, N2, and N3 treatments were 0.97, 1.04, and 1.07 g·(m2·h)-1, respectively, and these values were 10.2%, 18.2%, and 21.6% greater than that of N0[0.88 g·(m2·h)-1], respectively. The results from ANOVA testing indicated that nitrogen applications had no significant effect on the soil respiration rate during the non-growing season of alfalfa (P>0.05). According to the statistical analysis, the soil respiration rate had a significant exponential positive relationship with soil temperature during the growing season, non-growing season, and entire year of alfalfa grassland observations under different nitrogen application rates (P<0.01); the coefficients of determination were ranked as follows:growing season (0.46-0.62) < non-growing season (0.66-0.76) < whole year (0.80-0.86). Soil temperature (T) and soil moisture (W) interacted with each other and ultimately affected the soil respiration (RS), and by using a two-factor linear model of soil temperature and soil moisture, a better fit was obtained for the change in the soil respiration rate. Both of the two factors explained 68%-80% of the variation in the seasonal soil respiratory rate during the growing season of alfalfa. Nitrogen fertilization decreased the soil pH and available phosphorus content (AP) to varying degrees, but it increased the available potassium (AK), soil organic matter (SOM), and soil urease (URE) and invertase activity (INV). Total nitrogen (TN) and available nitrogen (AN) showed different trends under different nitrogen levels. The TN and AN contents increased considerably in soils; however, when the nitrogen rate was higher than N2 (120 kg·hm-2), TN and AN decreased with the increases in the nitrogen application rate. According to the correlation matrix analysis between soil respiration and soil biochemical properties during the growth period of alfalfa, data showed that the soil respiration rate (RS) was significantly and negatively correlated with soil pH (P<0.01), and it was significantly and positively correlated with soil TN and URE (P<0.01). Simultaneously, there was a significant positive correlation between the soil respiration rate (RS) and SOM (P<0.05), and there was a significant negative correlation with INV (P<0.05). The soil nutrient and enzyme activities of the alfalfa grassland explained the variations in the soil respiration rate under different nitrogen application levels to varying degrees.


Assuntos
Fertilizantes , Pradaria , Medicago sativa/crescimento & desenvolvimento , Nitrogênio/análise , Solo/química , China
12.
Huan Jing Ke Xue ; 40(10): 4708-4717, 2019 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854842

RESUMO

Based on the pattern of citrus tree/stropharia mushrooms intercropping, returning-straw was used as the raw material for the stropharia mushrooms, and an in-situ experiment was conducted to monitor soil CO2 emissions under different dosage of straw application during the stropharia growth period. Soil CO2 emissions and the influencing factors were analyzed under different treatments of cultivated (HSM, ASM, and DSM) and uncultivated stropharia mushrooms (HS, AS, and DS). The mushroom yield and soil carbon emission efficiency (CEE) were used to provide a theoretical basis for improving the use of land under citrus orchards. The results showed that:① Straw return increased the cumulative CO2 emissions compared with the control system (conventional planting, CK) and cumulative CO2 emissions increased with the dosage of straw application. Cumulative CO2 emissions from soil treated with cultivated stropharia mushrooms were higher than those from soil treated with uncultivated stropharia mushrooms, in the order of DSM (52.09 t·hm-2) > ASM (41.10 t·hm-2) > HSM (33.20 t·hm-2) > DS (27.15 t·hm-2) > AS (25.34 t·hm-2) > HS (18.94 t·hm-2) > CK (12.16 t·hm-2). Cumulative CO2 emissions under the DSM treatment significantly increased by 328.37% compared with CK. ② For the treatment of cultivated stropharia mushrooms, peak soil CO2 emissions occurred during the period of mycelium growth. The highest cumulative CO2 emissions during this period were obtained under the DSM treatment and accounted for 43.27% of the total cumulative emissions. This was followed by ASM and HSM which accounted for 42.63% and 40.57% of emissions, respectively. ③ Cultivated stropharia mushrooms reduced the temperature sensitivity coefficient (Q10). The soil temperature (5 cm depth) had a significant effect on the soil CO2 emission rate (P<0.01) but soil moisture did not (P>0.05). Soil temperature explained 27% to 71% of the variation in soil CO2 emissions rates, and the two-factor fitting of soil temperature and soil moisture explained 36% to 82% of the variation. ④ For the treatment of cultivated stropharia mushrooms, the ranked yield of each treatment was DSM (49.7 t·hm-2) > ASM (47.0 t·hm-2) > HSM (23.3 t·hm-2), and ASM had the highest soil CEE (1.14). Therefore, under the system of citrus tree/stropharia mushroom intercropping, straw return can increase soil CO2 emissions, with the highest emissions being obtained when a double dosage of straw was applied. However, the optimal amount of straw still needs to be determined in combination with changes in soil nutrients and crop yields.


Assuntos
Agaricales , Dióxido de Carbono , Citrus , Solo , Agricultura , China , Fertilizantes
13.
Zhongguo Fei Ai Za Zhi ; 22(11): 696-701, 2019 Nov 20.
Artigo em Chinês | MEDLINE | ID: mdl-31771738

RESUMO

BACKGROUND: The standard treatment for locally advanced non-small cell lung cancer (NSCLC) is radiotherapy concurrent with chemotherapy, but the survival was not satisfied. With the development of intensity modulated radiotherapy, simultaneous integrated boost technique (SIB) becomes the research direction of locally advanced NSCLC. The aim of this study is to investigate the efficacy and safety of SIB intensity modulated radiotherapy technique for locally advanced NSCLC. METHODS: We retrospectively reviewed the clinical data of locally advanced NSCLC who were treated with radiotherapy by SIB technique in Peking University Cancer Hospital from June 2015 to December 2018. Kaplan-Meier method was used for analysis. RESULTS: Ninty-three patients were included in the analysis. After a median follow-up of 34.23 months, 3-year overall survival (OS), progression-free survival (PFS), local-recurrence free survival (LRFS) and metastasis free survival (MFS) rates were 53.0%, 37.0%, 50.5% and 50.5%, respectively. The incidence of grade ≥3 esophagitis was 5.4%. There were 2 (2.2%) patients experiencing grade ≥3 radiation-related pneumonia. CONCLUSIONS: Radiation with SIB intensity modulated radiotherapy technique is effective and safe for patients with locally advanced NSCLC.

14.
Adv Sci (Weinh) ; 6(18): 1901166, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31559137

RESUMO

Mg2+-deficiency is linked to hypertension, Alzheimer's disease, stroke, migraine headaches, cardiovascular diseases, and diabetes, etc., but its exact role in these pathophysiological conditions remains elusive. Mg2+ can regulate vascular functions, yet the mechanistic insight remains ill-defined. Data show that extracellular Mg2+ enters endothelium mainly through the TRPM7 channel and MagT1 transporter. Mg2+ can act as an antagonist to reduce Ca2+ signaling in endothelium. Mg2+ also reduces the intracellular reactive oxygen species (ROS) level and inflammation. In addition, Mg2+-signaling increases endothelial survival and growth, adhesion, and migration. Endothelial barrier integrity is significantly enhanced with Mg2+-treatment through S1P1-Rac1 pathways and barrier-stabilizing mediators including cAMP, FGF1/2, and eNOS. Mg2+ also promotes cytoskeletal reorganization and junction proteins to tighten up the barrier. Moreover, Mg2+-deficiency enhances endothelial barrier permeability in mice, and Mg2+-treatment rescues histamine-induced transient vessel hyper-permeability in vivo. In summary, Mg2+-deficiency can cause deleterious effects in endothelium integrity, and Mg2+-treatment may be effective in the prevention or treatment of vascular dysfunction.

15.
Environ Toxicol Chem ; 38(12): 2698-2707, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31499584

RESUMO

Because of its hydrophobicity and persistence, dichlorodiphenyltrichloroethane (DDT) is ubiquitous in sediments and poses significant risk to benthic organisms. Therefore, it is imperative to evaluate the long-term toxicity of DDT. However, limited information is available on its chronic toxicity to benthic invertebrates. Full-life cycle toxicity of sediment-bound DDT to Chironomus dilutus was assessed. Median lethal concentrations (with 95% confidence limits) of DDT and its degradation products (DDX) to C. dilutus were 334 (165-568), 21.4 (11.2-34.3), and 7.50 (4.61-10.6) nmol/g organic carbon after 10-, 20-, and 63-d exposure, respectively. In addition, median effect concentrations of DDX were 20.0 (15.0-25.3), 7.13 (4.10-10.5), and 8.92 (3.32-15.1) nmol/g organic carbon for growth, emergence, and reproduction, respectively. A toxicity spectrum was established to visually summarize chronic effects of DDX to midges. In addition, DDT degraded to dichlorodiphenyldichloroethane (DDD) and dichlorodiphenyldichloroethylene (DDE) during sediment aging, and their toxicity differed from that of the parent compound. Predicted toxic units of DDX in porewater were utilized to distinguish between toxicity from DDT and that of DDD and DDE. The results showed that DDD was the main contributor to the toxicity in C. dilutus. To improve the accuracy of sediment risk assessment of DDT, the composition of DDX should be considered. Environ Toxicol Chem 2019;38:2698-2707. © 2019 SETAC.

16.
J Zhejiang Univ Sci B ; 20(10): 849-860, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31489804

RESUMO

We investigated the value of autoantibodies as biomarkers of chronic graft-versus-host disease (cGVHD) by analyzing the autoantibody profiles of 65 patients (34 cGVHD and 31 non-cGVHD) surviving longer than three months after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Autoantibodies to at least one antigen were detected in 45 patients (70.8%), with multiple autoantibodies detected in 30 patients (46.2%). Antinuclear antibodies (ANAs) were the most frequently detected autoantibodies, with a significantly higher prevalence in non-cGVHD patients and cGVHD patients than that in healthy controls (HCs). ANA-nucleolar (ANA-N) was the main immunofluorescence pattern of ANA-positivity in both the non-cGVHD and cGVHD groups. There was a higher prevalence of anti-Ro52-positivity in non-cGVHD and cGVHD patients than in HC. Liver cGVHD was significantly associated with anti-Ro52-positivity. However, cGVHD activity and severity were not associated with the presence of autoantibodies. Similarly, there were no significant differences in overall survival or relapse among the four groups of patients expressing autoantibodies. Our results suggest that autoantibodies have limited value in predicting cGVHD.

17.
Anesthesiology ; 131(4): 883-893, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31365367

RESUMO

BACKGROUND: Sympathetic dysfunction may be present in complex regional pain syndrome, and sympathetic blocks are routinely performed in practice. To investigate the therapeutic and predictive values of sympathetic blocks, the authors test the hypotheses that sympathetic blocks provide analgesic effects that may be associated with the temperature differences between the two extremities before and after the blocks and that the effects of sympathetic blocks may predict the success (defined as achieving more than 50% pain reduction) of spinal cord stimulation trials. METHODS: The authors performed a retrospective study of 318 patients who underwent sympathetic blocks in a major academic center (2009 to 2016) to assess the association between pain reduction and preprocedure temperature difference between the involved and contralateral limbs. The primary outcome was pain improvement by more than 50%, and the secondary outcome was duration of more than 50% pain reduction per patient report. The authors assessed the association between pain reduction and the success rate of spinal cord stimulation trials. RESULTS: Among the 318 patients, 255 were diagnosed with complex regional pain syndrome and others with various sympathetically related disorders. Successful pain reduction (more than 50%) was observed in 155 patients with complex regional pain syndrome (155 of 255, 61%). The majority of patients (132 of 155, 85%) experienced more than 50% pain relief for 1 to 4 weeks or longer. The degree and duration of pain relief were not associated with preprocedure temperature parameters with estimated odds ratio of 1.03 (97.5% CI, 0.95-1.11) or 1.01 (97.5% CI, 0.96-1.06) for one degree decrease (P = 0.459 or 0.809). There was no difference in the success rate of spinal cord stimulation trials between patients with or without more than 50% pain relief after sympathetic blocks (35 of 40, 88% vs. 26 of 29, 90%, P > 0.990). CONCLUSIONS: The authors conclude that sympathetic blocks may be therapeutic in patients with complex regional pain syndrome regardless of preprocedure limb temperatures. The effects of sympathetic blocks do not predict the success of spinal cord stimulation.


Assuntos
Bloqueio Nervoso Autônomo/métodos , Síndromes da Dor Regional Complexa/terapia , Adulto , Estudos de Coortes , Feminino , Humanos , Masculino , Estudos Retrospectivos , Resultado do Tratamento
18.
Cell Biol Int ; 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31329338

RESUMO

To investigate the roles of tripartite motif containing 52 (TRIM52) in human hepatic fibrosis in vitro, human hepatic stellate cell line LX-2 cells were transfected with hepatitis B virus (HBV) replicon to establish HBV-induced fibrosis in LX-2 cells, and then treated with small interfering RNA-mediated knockdown of TRIM52 (siTRIM52). LX-2 cells without HBV replicon transfection were treated with lentiviruses-mediated overexpression of TRIM52 and phosphatase magnesium dependent 1A (PPM1A). Fibrosis response of LX-2 cells were assessed by the production of hydroxyproline (Hyp) and collagen I/III, as well as protein levels of α-smooth muscle actin (α-SMA). PPM1A and phosphorylated (p)-Smad2/3 were measured to assess the mechanism. The correlation between TRIM52 and PPM1A was determined using co-immunoprecipitation, and whether and how TRIM52 regulated the degradation of PPM1A were determined by ubiquitination assay. Our data confirmed HBV-induced fibrogenesis of LX-2 cells, as evidenced by significant increase in Hyp and collagen I/III and α-SMA, which was associated with reduction of PPM1A and elevation of transforming growth factor-ß (TGF-ß), p-Smad2/3, and p-Smad3L. However, those changes induced by HBV were significantly attenuated with additional siTRIM52 treatment. Similar to HBV, overexpression of TRIM52 exerted promoted effect in the fibrosis of LX-2 cells. Interestingly, TRIM52 induced the fibrogenesis of LX-2 cells and the activation of TGF-ß/Smad pathway were significantly reversed by PPM1A overexpression. Furthermore, our data confirmed TRIM52 as a deubiquitinase that influenced the accumulation of PPM1A protein, and subsequently regulated the fibrogenesis of LX-2 cells. TRIM52 was a fibrosis promoter in hepatic fibrosis in vitro, likely through PPM1A-mediated TGF-ß/Smad pathway.

19.
Iran J Public Health ; 48(3): 379-387, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31223564

RESUMO

Background: Magnesium (Mg) is an essential element for the body. It is a cofactor for ATP, DNA, and RNA and more than 600 enzymes. As it is similar to Ca2+, this element can also act as a cell signaling molecule and play multiple important roles in the nervous, muscle, and immune systems. Recent studies have associated Mg-deficiency with many neurological disorders, such as cerebral vasospasm, Alzheimer's disease, stroke, and migraine. As it plays such a crucial role in human body, therefore, we summarized the role of Mg in neurological disorders to illustrate the symptoms caused by Mg-deficiency and the possible underlying mechanisms. Methods: We critically discuss the role of it that we review the recent literature of magnesium. We also review the available data which are concerning the role of magnesium in neurological disorders. Results: Magnesium is related to neurological disorders on the basis of the study of animals and humans experiments. Furthermore, these nervous systems related diseases include cerebral vasospasm, Alzheimer's disease, Parkinson's disease, stroke and migraine. Conclusion: Magnesium has effects on neurological disorders, such as its utility in cerebral vasospasm, Alzheimer's disease, Parkinson's disease, stroke and migraine. So here we make a brief review to conclude it.

20.
Kidney Blood Press Res ; 44(2): 245-263, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31071709

RESUMO

BACKGROUND/AIMS: Diabetic nephropathy (DN) is a leading cause of end-stage renal disease. Microalbuminuria (MA) is widely used to predict early progressive renal function decline (ERFD) of DN in type 2 diabetes mellitus (T2D) patients, but the sensitivity and specificity of MA have been questioned. Here, we determined the urine metabolites differences between T2D patients with MA who maintained stable renal function and those who progressed to ERFD in order to identify specific biomarkers of the progression of renal dysfunction. METHODS: A total of 102 T2D patients with MA and normal renal function at baseline were followed up for 5-6 years. Of these, 52 patients were selected and classified into two groups according to the later renal function; 25 patients who experienced ERFD were regarded as the progressive group, while 27 patients who maintained stable renal function were considered as the stable group. In the pilot study, untargeted, broad-spectrum urine metabolomics was performed on the urine of 12 subjects from the progressive group (5 patients as "progressors") and stable group (7 patients as "non-progressors") to discover candidate markers. We then used a targeted metabolomics analysis to identify the selected markers in the urine of an additional 40 patients (20 from the progressive group as cases, and 20 from the stable group as controls) in the validation study. RESULTS: A total of 318 known metabolites were detected in the pilot study and 6 metabolites with significant difference between progressors and non-progressors were identified. The levels of 4 metabolites, including azelaic acid, adipic acid, 5-hydroxyhexanoic acid, and L-tryptophan decreased significantly, while levels of L-pyroglutamic acid and D-norvaline increased observably in the progressors compared with non-progressors. Furthermore, in the validation study, 6 metabolites were confirmed by quantitative measurements and their concentrations were consistent with the changes in the pilot study. Concentrations of L-pyroglutamic acid and D-norvaline still increased in the cases, but were not statistically significant. Of the 4 metabolites with decreased concentrations among the cases, only 5-hydroxyhexanoic acid remained statistically significant while the other 3 metabolites did not differ between cases and controls. CONCLUSION: We have identified urine metabolites and shown that 5-hydroxyhexanoic acid can be used as a predictor of progression of ERFD in T2D patients with MA. This finding provides the new perspective that 5-hydroxyhexanoic acid may be useful to identify T2D patients with MA who are at risk of ERFD.


Assuntos
Albuminúria/urina , Caproatos/urina , Diabetes Mellitus Tipo 2/urina , Nefropatias Diabéticas/urina , Progressão da Doença , Hidroxiácidos/urina , Adulto , Biomarcadores/urina , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/complicações , Feminino , Seguimentos , Humanos , Nefropatias/urina , Masculino , Metabolômica/métodos , Pessoa de Meia-Idade
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