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1.
Front Biosci (Landmark Ed) ; 24: 1477-1486, 2019 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-31136992

RESUMO

Diabetic nephropathy (DN) is one of the most serious complications of diabetes mellitus, for which no effective treatment currently exists. We tested the hypothesis that Qi-dan-di-huang (QDDH) might have therapuetic effects in an experimental rat model of DN. The levels of I kappa KinaseAlpha and Beta, p-p65, p-IκB alpha, TGF-ß1 and Alpha-SMA were significantly increased in kidneys in DN. QDDH decoction only partially reversed the increased Ikappa KinaseAlpha/Beta, p-p65, p-IKappaB alpha, TGF-Beta1 and alpha-SMA in the kidneys in DN. However, treatment of diabetic rats with QDDH decoction significantly inhibited the production and release of inflammatory cytokines IL-6, IL-1 beta and TNF-alpha into the serum. QDDH decoction also significantly improved the physiologic and biochemical indicators of DN, reduced glycogen and protein deposition in DN and prevented renal fibrosis. Together, the data show that QDDH decoction exerts a protective effect on kidneys in diabetic rats and reverses the inflammatory milieu of the serum in DN.


Assuntos
Diabetes Mellitus Experimental/complicações , Nefropatias Diabéticas/prevenção & controle , Medicamentos de Ervas Chinesas/farmacologia , NF-kappa B/metabolismo , Transdução de Sinais/efeitos dos fármacos , Animais , Citocinas/sangue , Nefropatias Diabéticas/complicações , Nefropatias Diabéticas/metabolismo , Fibrose , Glicogênio/metabolismo , Humanos , Mediadores da Inflamação/sangue , Rim/efeitos dos fármacos , Rim/metabolismo , Rim/patologia , Masculino , Ratos Sprague-Dawley
2.
Nan Fang Yi Ke Da Xue Xue Bao ; 39(2): 227-234, 2019 02 28.
Artigo em Chinês | MEDLINE | ID: mdl-30890513

RESUMO

OBJECTIVE: To identify the main active components in Shenbing decoction Ⅲ and their targets and explore the mechanism by which Shenbing decoction Ⅲ alleviates proteinuria in chronic kidney disease (CKD) based on network pharmacology. METHODS: The active components of Shenbing decoction Ⅲ and their potential targets, along with the oral bioavailability and drug-like properties of each component were searched in the TCMSP database. The proteinuria-related targets were searched in the GeneCards database. The active component-target network was constructed using Cytoscape software, and the acquired information of the targets from ClueGO was used for enrichment analysis of the gene pathways. RESULTS: A total of 102 active components were identified from Shenbing decoction Ⅲ. These active components acted on 126 targets, among which 69 were related to proteinuria. Enrichment analysis revealed fluid shear stress- and atherosclerosisrelated pathways as the highly significant pathways in proteinuria associated with CKD. CONCLUSIONS: We preliminarily validated the prescription of Shenbing decoction Ⅲ and obtained scientific evidence that supported its use for treatment of proteinuria in CKD. The findings in this study provide a theoretical basis for further study of the mechanism of Shenbing decoction Ⅲ in the treatment of proteinuria in CKD.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Proteinúria/tratamento farmacológico , Insuficiência Renal Crônica/complicações , Disponibilidade Biológica , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacocinética , Humanos , Proteinúria/etiologia , Proteinúria/metabolismo , Insuficiência Renal Crônica/metabolismo
3.
Ecotoxicol Environ Saf ; 166: 11-17, 2018 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-30240930

RESUMO

Effluents loaded with various synthetic dyes are considered as a huge burden to the surrounding ecosystems. Sudan dyes are relatively difficult to decolorize due to its water-insolubility. In the present study, the strain Shewanella putrefaciens CN32 was firstly applied to decolorize Sudan dyes under the anaerobic condition, and the physicochemical parameters on the decolorization were optimized. The results demonstrated that the suitable decolorization condition was temperature 26 °C, initial pH 7.0-8.0 and NaCl concentrations 0-20 g/L. Electron competitive acceptors including nitrite, nitrate, dimethyl sulphoxide and oxygen could cause the significant inhibition to the decolorization of Sudan dyes. Biosurfactant rhamnolipid played a positive role in enhancing the decolorization of Sudan I. The co-culture of S. putrefaciens CN32 and Bacillus circulans BWL1061 is reported for the first time to accelerate the decolorization through improving the synergistic effect of enzymatic degradation and biological reductive effect. The highest decolorization of 90.23% to Sudan I was achieved within 108 h, suggesting that co-culture technique has a good potential in the treatment of dyeing wastewater. Furthermore, the microbial toxicity tests indicated that the toxicity of Sudan I to Escherichia coli BL21 and Bacillus subtilis 168 was obviously decreased after the decolorization.


Assuntos
Compostos Azo/metabolismo , Bacillus/metabolismo , Biodegradação Ambiental , Corantes/metabolismo , Shewanella putrefaciens/metabolismo , Poluentes Químicos da Água/metabolismo , Compostos Azo/análise , Compostos Azo/química , Técnicas de Cocultura , Corantes/química , Águas Residuárias/química , Água/química , Poluentes Químicos da Água/análise
4.
Int Immunopharmacol ; 55: 174-182, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29268189

RESUMO

In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLSs) play an essential role in cartilage destruction. Aggressive migration and invasion by FLSs significantly affect RA pathology. Kaempferol has been shown to inhibit cancer cell migration and invasion. However, the effects of kaempferol on RA FLSs have not been investigated. Our study aimed to determine the effects of kaempferol on RA both in vitro and in vivo. In vitro, cell migration and invasion were measured using scratch assays and the Boyden chamber method, respectively. The cytoskeletal reorganization of RA FLSs was evaluated by immunofluorescence staining. Matrix metalloproteinase (MMP) levels were measured by real-time PCR, and protein expression levels were measured by western blotting. In vivo, the effects of kaempferol were evaluated in mice with CIA. The results showed that kaempferol reduced migration, invasion and MMP expression in RA FLSs. In addition, we demonstrated that kaempferol inhibited reorganization of the actin cytoskeleton during cell migration. Moreover, kaempferol dramatically suppressed tumor necrosis factor (TNF)-α-induced MAPK activation without affecting the expression of TNF-α receptors. We also demonstrated that kaempferol attenuated the severity of arthritis in mice with CIA. Taken together, these results suggested that kaempferol inhibits the migration and invasion of FLSs in RA by blocking MAPK pathway activation without affecting the expression of TNF-α receptors.


Assuntos
Antirreumáticos/uso terapêutico , Artrite Reumatoide/tratamento farmacológico , Fibroblastos/fisiologia , Quempferóis/uso terapêutico , Sinoviócitos/fisiologia , Actinas/metabolismo , Adulto , Idoso , Animais , Artrite Experimental , Movimento Celular , Células Cultivadas , Citoesqueleto/metabolismo , Feminino , Humanos , Sistema de Sinalização das MAP Quinases , Masculino , Camundongos , Camundongos Endogâmicos DBA , Pessoa de Meia-Idade , Fator de Necrose Tumoral alfa/metabolismo
5.
Ecotoxicol Environ Saf ; 145: 8-15, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28689070

RESUMO

Dye dispersion and the interaction efficiency between azoreductases and dye molecules are rate-limiting steps for the decolorization of azo dyes. In this study, a biosurfactant-producing strain, Pseudomonas taiwanensis L1011, was isolated from crude oil. To increase the yield of the biosurfactant BS-L1011 from P. taiwanensis L1011, culture conditions were optimized including temperature, initial pH, carbon source, nitrogen source and C/N ratio. A maximum yield of 1.12g/L of BS-L1011 was obtained using D-mannitol as carbon source and yeast extract/urea as compound nitrogen source with C/N ratio of 10/4, pH 7.0 and 28°C. BS-L1011 exhibited a low critical micelle concentration (CMC) of 10.5mg/L and was able to reduce the surface tension of water to 25.8±0.1 mN/m. BS-L1011 was stable over a wide range of temperatures, pH values and salt concentrations. The biosurfactant is reported for the first time to accelerate chemical decolorization of Congo red by sodium hypochlorite, and biological decolorization of Amaranth by Bacillus circulans BWL1061, thus showing a potential in the treatment of dyeing wastewater.


Assuntos
Compostos Azo/análise , Corantes/análise , Pseudomonas/metabolismo , Tensoativos/química , Poluentes Químicos da Água/análise , Corante Amaranto/análise , Biodegradação Ambiental , Vermelho Congo/análise , NADH NADPH Oxirredutases/química , Petróleo/microbiologia , Pseudomonas/crescimento & desenvolvimento , Pseudomonas/isolamento & purificação , Tensoativos/isolamento & purificação , Águas Residuárias/química
6.
Mol Med Rep ; 16(3): 2379-2388, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28677739

RESUMO

Mitochondrial dysfunction is closely associated with the pathogenesis of nonalcoholic steatohepatitis (NASH). The aim of the present study was to comprehensively determine mitochondrial abnormalities in NASH by detecting the proteomics in liver mitochondria in a NASH rat model, which was induced for 16 weeks by the provision of a high fat and high cholesterol diet (HFD). Serum parameters, including triglycerides, total cholesterol, low­density lipoprotein cholesterol and high­density lipoprotein cholesterol were determined, and hematoxylin and eosin staining of liver tissues was examined to evaluate the NASH rat model. Various parameters associated with mitochondrial function were examined, including mitochondrial DNA (mtDNA) copy number, mitochondrial membrane potential (MMP) and mitochondrial respiratory chain complex (MRC) activity. The mitochondrial proteomics were analyzed and identified using isobaric tags for relative and absolute quantitation labeling coupled with two­dimensional liquid chromatography­tandem mass spectrometry. The identified proteins were classified and grouped using the Blast2GO program against the non­redundant protein database, the Kyoto Encyclopedia of Genes and Genomes database and the Cluster of Orthologous Groups of proteins database. Compared with the control, mtDNA copy number, MMP, and activities of MRC I and III were decreased markedly in the HFD group. A total of 18 upregulated and 13 downregulated proteins were identified, with a significant 1.2­fold difference between the control and NASH groups. The dysregulated proteins were closely involved in mitochondrial oxidative phosphorylation, the lipid metabolic process and fatty acid ß­oxidation. The results of the present study provide important proteomic information regarding liver mitochondria in NASH and serve as a basis for further detailed investigations of the pathogenesis of NASH.


Assuntos
Mitocôndrias Hepáticas/patologia , Proteínas Mitocondriais/metabolismo , Hepatopatia Gordurosa não Alcoólica/patologia , Animais , DNA Mitocondrial/genética , Dosagem de Genes , Regulação da Expressão Gênica , Masculino , Potencial da Membrana Mitocondrial , Mitocôndrias Hepáticas/genética , Mitocôndrias Hepáticas/metabolismo , Proteínas Mitocondriais/análise , Proteínas Mitocondriais/genética , Hepatopatia Gordurosa não Alcoólica/genética , Hepatopatia Gordurosa não Alcoólica/metabolismo , Proteômica , Ratos , Ratos Sprague-Dawley
7.
Ecotoxicol Environ Saf ; 141: 9-16, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28284151

RESUMO

The co-existence of dyes, Cr(VI) and high concentration of salt in dyeing wastewater causes serious and complex environmental problems. In this study, a salt-tolerant strain Bacillus circulans BWL1061 was reported to simultaneously remove 50mg/L methyl orange and 50mg/L Cr(VI) under the anaerobic condition with 60g/L NaCl. During the decolorization process, the Cr(VI) reduction occurred preferentially over the dye decolorization due to the dominate utilization of electron by Cr(VI). The analysis of enzyme activities suggested that azoreductase, NADH-DCIP reductase, and laccase were associated with decolorization of methyl orange. A possible degradation pathway was proposed based on the metabolites analysis. The decolorization of methyl orange is involved in the symmetric cleavage of azo bond, which formed N,N-dimethyl p-phenylenediamine and 4-amino sulfonic acid, or the asymmetric cleavage of azo bond, which formed 4-(dimethylamino) phenol and 4-diazenylbenzene sulfonic acid. Phytotoxicity assays showed that strain BWL1061 could decrease the toxicity of methyl orange to Triticum aestivum, Pogostemon cablin and Isatis indigotica Fort during the decolorization process. In this study, Bacillus circulans is reported for the first time that could simultaneously remove azo dyes and Cr (VI) under high salt condition.


Assuntos
Compostos Azo/análise , Bacillus/crescimento & desenvolvimento , Cromo/análise , Corantes/análise , Tolerância ao Sal , Purificação da Água/métodos , Anaerobiose , Compostos Azo/química , Bacillus/enzimologia , Bacillus/metabolismo , Biodegradação Ambiental , Cromo/química , Corantes/química , Lacase/metabolismo , NADH NADPH Oxirredutases/metabolismo , Quinona Redutases/metabolismo , Cloreto de Sódio/metabolismo , Águas Residuárias/química
8.
Biochim Biophys Acta ; 1862(4): 829-836, 2016 04.
Artigo em Inglês | MEDLINE | ID: mdl-26705675

RESUMO

Haplogroup G predisposes one to an increased risk of osteoarthritis (OA) occurrence, while haplogroup B4 is a protective factor against OA onset. However, the underlying mechanism is not known. Here, by using trans-mitochondrial technology, we demonstrate that the activity levels of mitochondrial respiratory chain complex I and III are higher in G cybrids than in haplogroup B4. Increased mitochondrial oxidative phosphorylation (OXPHOS) promotes mitochondrial-related ATP generation in G cybrids, thereby shifting the ATP generation from glycolysis to OXPHOS. Furthermore, we found that lower glycolysis in G cybrids decreased cell viability under hypoxia (1% O2) compared with B4 cybrids. In contrast, G cybrids have a lower NAD(+)/NADH ratio and less generation of reactive oxygen species (ROS) under both hypoxic (1% O2) and normoxic (20% O2) conditions than B4 cybrids, indicating that mitochondrial-mediated signaling pathways (retrograde signaling) differ between these cybrids. Gene expression profiling of G and B4 cybrids using next-generation sequencing technology showed that 404 of 575 differentially expressed genes (DEGs) between G and B4 cybrids are enriched in 17 pathways, of which 11 pathways participate in OA. Quantitative reverse transcription PCR (qRT-PCR) analyses confirmed that G cybrids had lower glycolysis activity than B4 cybrids. In addition, we confirmed that the rheumatoid arthritis pathway was over-activated in G cybrids, although the remaining 9 pathways were not further tested by qRT-PCR. In conclusion, our findings indicate that mtDNA haplogroup G may increase the risk of OA by shifting the metabolic profile from glycolysis to OXPHOS and by over-activating OA-related signaling pathways.


Assuntos
DNA Mitocondrial/genética , Haplótipos , Mitocôndrias/genética , Proteínas Mitocondriais/genética , Osteoartrite/genética , Transdução de Sinais/genética , Linhagem Celular Tumoral , DNA Mitocondrial/metabolismo , Feminino , Humanos , Masculino , Mitocôndrias/metabolismo , Mitocôndrias/patologia , Proteínas Mitocondriais/metabolismo , Osteoartrite/metabolismo , Fosforilação Oxidativa , Fatores de Risco
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