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1.
ChemSusChem ; 12(22): 5007-5014, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31468722

RESUMO

The promising tin perovskite solar cells (PSCs) suffer from the oxidation of Sn2+ to Sn4+ , leading to a disappointing conversion efficiency along with poor stability. In this work, phenylethylammonium bromide (PEABr) was employed to form an ultrathin, low-dimensional perovskite layer on the surface of the FASnI3 (FA=formamidinium) absorber film to improve the interface of perovskite/PCBM ([6,6]-phenyl-C61 -butyricacid methyl) in the inverted planar device structure of the ITO (indium-doped tin oxide)/PEDOT:PSS [poly(3,4-ethylenedioxythiophene)/polystyrene sulfonate]/perovskite/[6,6]-phenyl-C61 -butyricacid methyl (PCBM)/BCP (2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) electrode. The device efficiency was enhanced from 4.77 to 7.86 % by this PEABr treatment. A series of characterizations proved that this modification could improve the crystallinity of the FASnI3 perovskite by incorporating Br and forming an ultrathin, low-dimensional perovskite layer at the interface, which led to the effective suppression of Sn2+ oxidation, improved band level alignment, and decreased defect density. These effects contributed to the clear enhancement of conversion efficiency. Moreover, this treatment also led to remarkably enhanced device stability, with approximately 80 % of the initial efficiency retained after 350 h light soaking, whereas the control device failed within 140 h. This work deepens our understanding of the suppression effect of PEABr on the oxidation of Sn2+ and paves a new way to fabricate promising tin halide PSCs by facile interface engineering.

2.
Cancer Manag Res ; 10: 4125-4134, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30323668

RESUMO

Purpose: The present study aimed to study the role of autophagy in the radiosensitivity of the radioresistant human nasopharyngeal carcinoma cell line CNE-2R. Methods: Before being irradiated, CNE-2R cells were treated with the autophagy inhibitor chloroquine diphosphate (CDP) or the autophagy inducer rapamycin (RAPA). Microtubule-associated protein light chain 3 (LC3-II) and p62 were assessed using Western blotting analysis 48 hours after CNE-2R cells were irradiated. The percentage of apoptotic cells was assessed via flow cytometry. CNE-2R cell viability was evaluated using the Cell Counting Kit-8 (CCK8). The radiosensitivity of cells was assessed via clone formation analysis. Results: The level of autophagy in CNE-2R cells improved as the radiation dose increased, reaching the maximum at a dose of 10 Gy. Autophagy was most significantly inhibited by 60 µmol/L CDP in CNE-2R cells, but was obviously enhanced by 100 nmol/L RAPA. Compared with the irradiation (IR) alone group, in the IR + CDP group, autophagy was significantly inhibited, viability was low, the rate of radiation-induced apoptosis was increased, and radiosensitivity was upregulated. In contrast, cells of the IR + RAPA group exhibited greater autophagy, higher viability, a lower rate of radiation-induced apoptosis, and downregulated radiosensitivity. Conclusion: The autophagy level is negatively correlated with radiosensitivity for the radio-resistant human nasopharyngeal carcinoma cell line CNE-2R.

3.
Cancer Med ; 7(9): 4755-4764, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30105829

RESUMO

The radioresistance of nasopharyngeal carcinoma (NPC) may be related to cancer stem cells (CSCs), and the characteristics of CSCs may be maintained by telomerase activity. In this study, we explored the CSC-like characteristics and telomerase activity of the NPC radioresistant cell line CNE-2R. This work provides a foundation for future studies on stem cell-targeted therapies by targeting the radioresistance of NPC. The expression of stem cell-related genes/proteins and the hTERT gene/protein in CNE-2R and its parent radiosensitive cell line CNE-2 were detected using qPCR/Western Blot. Label-retaining cells (LRCs) were detected through immunocytochemistry, and telomerase activity was detected using a PCR-ELISA kit. CD133 expression was detected with flow cytometry. CNE-2R-CD133+ and CNE-2R-CD133- cells were separated with magnetic-activated cell sorting. The proliferation and tumorigenesis capacities of CNE-2R-CD133+, CNE-2R-CD133-, and CNE-2R cells were compared with a CCK-8 assay, sphere formation assay, and an in vivo experiment. Our results showed that the expression of stem cell-related genes and the hTERT gene in CNE-2R cells was higher than those in CNE-2 cells. Similarly, the expression of stem cell-related proteins and the hTERT protein in CNE-2R cells was markedly higher than those in CNE-2 cells. The proportion of LRCs in CNE-2R and CNE-2 cells was (3.10 ± 0.63%) vs (0.40 ± 0.35%; P < 0.001), respectively. Telomerase activity in CNE-2R cells was remarkably higher than that in CNE-2 cells. Flow cytometry suggested that the CD133 positive rates in CNE-2R and CNE-2 cells were (2.49 ± 0.56%) vs (0.76 ± 0.25%; P = 0.008), respectively. Meanwhile, the proliferation capacity, tumorigenesis capacity, and telomerase activity of CNE-2R-CD133+ cells were notably higher than those of CNE-2R-CD133- and CNE-2R cells. Collectively, CNE-2R displayed CSC-like characteristics; our results also showed that CNE-2R cells, especially the sorted CSCs, had high telomerase activity levels.


Assuntos
Antineoplásicos/farmacologia , Resistencia a Medicamentos Antineoplásicos , Carcinoma Nasofaríngeo/enzimologia , Células-Tronco Neoplásicas/efeitos dos fármacos , Células-Tronco Neoplásicas/metabolismo , Telomerase/metabolismo , Apoptose/efeitos dos fármacos , Apoptose/genética , Biomarcadores Tumorais , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Movimento Celular/genética , Proliferação de Células/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/genética , Ativação Enzimática , Humanos , Carcinoma Nasofaríngeo/tratamento farmacológico , Células-Tronco Neoplásicas/patologia , Proteoma , Proteômica/métodos , Telomerase/genética
4.
Med Sci Monit ; 24: 2317-2329, 2018 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-29664897

RESUMO

BACKGROUND The purpose of this study was to determine whether cofilin-2 could serve as a protein marker for predicting radiotherapy response and as a potential therapeutic target in nasopharyngeal carcinoma (NPC). MATERIAL AND METHODS Cofilin-2 protein levels in serum and tissue samples from patients with NPC were assessed by sandwich ELISA and IHC. In vitro, cofilin-2 levels in CNE-2R cells were significantly higher than those of CNE-2 cells. Meanwhile, CNE-2R cells were silenced for cofilin-2 to obtain a stable cofilin-2-RNAi-LV3 cell line. Then, cell proliferation, radiosensitivity, invasion and migration abilities, cell cycle, and apoptosis were evaluated by Cell Counting Kit 8 assay (CCK-8), flow cytometry (FCM), clone formation assay, and in vitro. RESULTS The secreted levels of the cofilin-2 protein in radioresistant NPC patients were significantly higher than those of radiosensitive cases. After cofilin-2 knockdown in nasopharyngeal carcinoma CNE-2R cells, proliferation was decreased, while apoptosis and radiosensitivity were enhanced; cell cycle distribution was altered, and the transplanted tumors in nude mice grew significantly less. CONCLUSIONS Overall, our findings suggest that cofilin-2 acts as a marker for predicting radiotherapy response and is a potential therapeutic target in nasopharyngeal carcinoma.


Assuntos
Carcinoma/metabolismo , Carcinoma/radioterapia , Cofilina 2/metabolismo , Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/radioterapia , Animais , Apoptose/efeitos da radiação , Biomarcadores Tumorais/sangue , Biomarcadores Tumorais/metabolismo , Carcinoma/genética , Carcinoma/patologia , Pontos de Checagem do Ciclo Celular/efeitos da radiação , Linhagem Celular Tumoral , Proliferação de Células/efeitos da radiação , Cofilina 2/sangue , Cofilina 2/genética , Feminino , Técnicas de Silenciamento de Genes , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/genética , Neoplasias Nasofaríngeas/patologia , Valor Preditivo dos Testes , Tolerância a Radiação , Ensaios Antitumorais Modelo de Xenoenxerto
5.
Oncotarget ; 9(3): 3230-3241, 2018 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-29423042

RESUMO

Radioresistance is a major cause leads to treatment failure in nasopharyngeal carcinoma (NPC). In our previous study, we identified that QSOX1 is a differentially expressed protein in NPC cell lines with variable radiosensitivities. The present study aimed to investigate the biological behavior of QSOX1 in nasopharyngeal carcinoma (NPC) and its effect on radiosensitivity. The levels of QSOX1 detected by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in radioresistant NPC patient sera and tissue samples were markedly lower than those in radiosensitive samples. Small hairpin RNAs (shRNAs) were employed to knock down endogenous QSOX1 expression in CNE-2 cells, and then, radiosensitivity, apoptosis, migration and invasion were assessed using colony formation, Cell Counting Kit-8 (CCK-8), flow cytometry, and transwell assays, respectively. Tumor growth and radioresistance were also evaluated using a xenograft model in nude mice. The shRNA-mediated knockdown of QSOX1 significantly increased cell survival under irradiation (IR) and weakened radiosensitivity, which was likely due to a reduction in the cell apoptosis rate after IR. Moreover, QSOX1 silencing led to the suppression of cellular migration and invasion. Similar results were obtained with the xenograft mouse model. Thus, targeting QSOX1 will provide a new avenue for increasing the sensitivity of NPC to radiotherapy.

6.
Sci Rep ; 7: 41449, 2017 02 02.
Artigo em Inglês | MEDLINE | ID: mdl-28150694

RESUMO

The present study aimed to define high-risk patients who may benefit from additional adjuvant chemotherapy (AC) after concurrent chemotherapy in combination with intensity-modulated radiotherapy among patients with loco-regionally advanced nasopharyngeal carcinoma (NPC). A cohort of 511 NPC patients who received concomitant chemoradiotherapy (CCRT) with or without AC between January 2007 and December 2012 were retrospectively analysed. One hundred seventy-seven patients received CCRT alone, whereas 334 received CCRT + AC. The survival analysis showed that ages >45 years old, T3-T4 stages, N2-N3 disease and serum albumin levels ≤42 g/L were significant independent prognostic factors for overall survival (OS). Using these four risk factors, a prognostic model for OS was created as follows: (1) low-risk group: 0-1 risk factors; and (2) high-risk group: 2-4 risk factors. In the CCRT alone and CCRT + AC groups, significant differences in survival were found between the high- and low-risk groups. Patients in the high-risk group exhibited improved OS due to the addition of AC to CCRT, but no survival benefits were found in the low-risk group. In conclusion, high-risk patients may benefit from the addition of AC to CCRT regarding OS.


Assuntos
Carcinoma/patologia , Carcinoma/terapia , Quimiorradioterapia , Neoplasias Nasofaríngeas/patologia , Neoplasias Nasofaríngeas/terapia , Adulto , Quimioterapia Adjuvante , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo , Estadiamento de Neoplasias , Curva ROC , Fatores de Risco , Análise de Sobrevida
7.
Zhonghua Nan Ke Xue ; 23(3): 267-270, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29706050

RESUMO

Gap junctions (GJ), as a special membrane structure between adjacent cells, are composed of connexins (Cx) and regulate the proliferation and differentiation of cells. Studies show that gap junctional intercellular communication is weakened or lost in most tumor cells and this abnormality is often accompanied by changed expression of Cxs. Cx43 is a major connexin in the testis tissue. This review focuses on the latest progress in the studies of Cx43 in testicular tumors.


Assuntos
Comunicação Celular , Conexina 43/metabolismo , Junções Comunicantes/metabolismo , Neoplasias Testiculares/metabolismo , Animais , Diferenciação Celular , Masculino
8.
Nano Lett ; 15(5): 3088-95, 2015 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-25929671

RESUMO

The key challenges in enhancing the power conversion efficiency (PCE) of a quantum dot-sensitized solar cell (QDSSC) are efficiently achieving charge separation at the photoanode and improving the charge transfer, which is limited by the interface between the electrolyte and the counter electrode (CE). Here, hierarchically assembled ITO@Cu2S nanowire arrays with conductive single-crystalline ITO cores and Cu2S nanocrystal shells were designed as efficient QDSSCs CEs. These arrays not only provided an efficient three-dimensional charge transport network but also allowed for the effective deposition of more Cu2S nanocrystals as active sites to catalyze the electrolyte reaction. This design considerably reduced the sheet and charge transfer resistance of the CE, thus decreasing the series resistance and increasing the shunt resistance of the QDSSC. As a result, QDSSCs with this CE exhibited an unprecedentedly high Voc of 0.688 V, a fill factor of 58.39%, and a PCE of 6.12%, which is 21.2% higher than that of the conventional brass/Cu2S CE.

9.
J Am Chem Soc ; 137(6): 2211-4, 2015 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-25646928

RESUMO

We report here the selective synthesis of air-stable phase-pure pyrite FeS2 nanocubes, spheroidal nanocrystals, and microspheres by solvent-induced oriented attachment (OA). It was found that the solvents could control the OA process and thus the morphologies of the products. Solvent exchange experiments and detailed Raman analysis revealed that 1-octanol contributed to the long-term stability of these pyrite nanomaterials.

10.
ACS Appl Mater Interfaces ; 6(17): 15448-55, 2014 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-25137502

RESUMO

Among the issues that restrict the power conversion efficiency (PCE) of quantum-dot-sensitized solar cells (QDSSCs), insufficient catalytic activity and stability of counter electrodes (CEs) are critical but challenging ones. The state-of-the-art Cu/Cu2S CEs still suffer from mechanical instability and uncertainty due to the reaction of copper and electrolyte. Herein, ITO@Cu2S core-shell nanowire arrays were developed to fabricate CEs for QDSSCs, which have no such issues in Cu/Cu2S CEs. These nanowire arrays exhibited small charge transfer resistance and sheet resistance, and provided more active catalytic sites and easy accessibility for electrolyte due to the three-dimensional structure upon use as CEs. More interestingly, it was found that the interface of ITO/Cu2S significantly affected the performance of ITO@Cu2S nanowire array CEs. By varying synthetic methods, a series of ITO@Cu2S nanowire arrays were prepared to investigate the influence of ITO/Cu2S interface on their performance. The results showed that ITO@Cu2S nanowire array CEs with a continuous Cu2S nanocrystal shell fabricated via an improved cation exchange route exhibited excellent and thickness-dependent performance. The PCE of corresponding QDSSCs increased by 11.6 and 16.5% compared to that with the discrete Cu2S nanocrystal and the classic Cu/Cu2S CE, respectively, indicating its promising potential as a new type of CE for QDSSCs.

11.
Nanoscale ; 6(17): 9939-43, 2014 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-25054637

RESUMO

We synthesize Au@Ag core/shell nanoparticles (NPs) using a Au NP assisted Tollens reaction. The as-synthesized NPs are used for the colorimetric cyanide sensing with a detection limit of 0.4 µM. The bimetallic NPs are immobilized into agarose gels as portable "test strips".

12.
Zhonghua Nan Ke Xue ; 20(5): 400-4, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-24908728

RESUMO

OBJECTIVE: To investigate the effects of total flavonoids of Litsea Coreana (TFLC) on the gap junction (GJ) intercellular communication in TM3 testicular Leydig cells and whether TFLC can reduce the cytotoxicity of oxaliplatin (OHP) in vitro. METHODS: We detected the effect of TFLC on the dye spread of the in vitro cultured TM3 cells by parachute assay, observed changes in the expression of connexin 43 (Cx43) total protein in the TFLC-treated TM3 cells by Western blot, and determined the effects of TFLC on the expression of Cx43 on the membrane of the TM3 cells by immunofluorescence assay and on the cytotoxicity of OHP by MTT assay. RESULTS: TFLC obviously enhanced the GJ function with the increasing of the TFLC concentration in the TM3 cells. Western blot and immunofluorescence assay confirmed that TFLC significantly enhanced the expression of Cx43 total protein and Cx43 expression on the membrane of the TM3 cells. MTT assay showed that at a high cell density (confluent with GJ formation), 20 microg/ml TFLC enhanced the GJ function of the TM3 cells and reduced the cytotoxicity of OHP (P < 0.05), while at a low density (preconfluent with no GJ formation), TFLC exhibited no effect on the cytotoxicity of OHP (P > 0.05). CONCLUSION: TFLC increases the Cx43 expression and GJ function in normal TM3 Leydig cells, and the enhancement of GJ function reduces the cytotoxicity of OHP.


Assuntos
Antineoplásicos , Comunicação Celular/efeitos dos fármacos , Conexina 43/metabolismo , Flavonoides/farmacologia , Junções Comunicantes/efeitos dos fármacos , Células Intersticiais do Testículo/efeitos dos fármacos , Litsea/química , Compostos Organoplatínicos/antagonistas & inibidores , Antineoplásicos/toxicidade , Comunicação Celular/fisiologia , Contagem de Células , Humanos , Técnicas In Vitro , Células Intersticiais do Testículo/ultraestrutura , Masculino , Compostos Organoplatínicos/toxicidade , Oxaliplatina , Proteínas/metabolismo
13.
Biol Pharm Bull ; 37(8): 1315-22, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24871203

RESUMO

Oxaliplatin is widely used in the treatment of variety of cancers, including cancer of the testis and colorectum. Gap junctions (GJs) can amplify the cytotoxicity of antinoeoplastic drugs through the bystander effect in different cancer cells. In this study, we demonstrate that total flavonoids of litsea coreana (TFLC), one extract from the dried leaves of litsea coreana leve, increase the cytotoxicity of oxaliplatin in mouse testicular cancer I-10 cells. We found that cell survival was substantially decreased only when functional GJs formed in I-10 cells. TFLC increased oxaliplatin cytotoxity (inducing cell death and apoptosis) by enhancing gap junction intercellular communication (GJIC) through elevated Cx43 protein expression. Furthermore, apoptosis-related protein (Bax, Bcl-2, caspase-3/9) results showed that the Bax/Bcl-2 ratio and activated caspase-3/9 increased when TFLC was used compared with treatment with oxaliplatin alone, which suggests that the mechanism of increased oxaliplatin-induced apoptosis was through the mitochondrial pathway. These results demonstrate that TFLC can enhance the cytotoxicity of oxaliplatin, and that these processes may be regulated in testicular tumor cells through GJ-mediated regulation of tumor cell apoptosis.


Assuntos
Antineoplásicos/farmacologia , Flavonoides/farmacologia , Junções Comunicantes/efeitos dos fármacos , Litsea , Compostos Organoplatínicos/farmacologia , Animais , Caspase 3/metabolismo , Caspase 9/metabolismo , Comunicação Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Conexina 43/metabolismo , Sinergismo Farmacológico , Junções Comunicantes/fisiologia , Camundongos , Oxaliplatina , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Proteína X Associada a bcl-2/metabolismo
14.
Nano Lett ; 14(1): 365-72, 2014 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-24350879

RESUMO

Quantum-dot-sensitized solar cell (QDSSC) has been considered as an alternative to new generation photovoltaics, but it still presents very low power conversion efficiency. Besides the continuous effort on improving photoanodes and electrolytes, the focused investigation on charge transfer at interfaces and the rational design for counter electrodes (CEs) are recently receiving much attention. Herein, core-shell nanowire arrays with tin-doped indium oxide (ITO) nanowire core and Cu2S nanocrystal shell (ITO@Cu2S) were dedicatedly designed and fabricated as new efficient CEs for QDSSCs in order to improve charge collection and transport and to avoid the intrinsic issue of copper dissolution in popular and most efficient Cu/Cu2S CEs. The high-quality tunnel junctions formed between n-type ITO nanowires and p-type Cu2S nanocrystals led to the considerable decrease in sheet resistance and charge transfer resistance and thus facilitated the electron transport during the operation of QDSSCs. The three-dimensional structure of nanowire arrays provided high surface area for more active catalytic sites and easy accessibility for an electrolyte. As a result, the power conversion efficiency of QDSSCs with the designed ITO@Cu2S CEs increased by 84.5 and 33.5% compared to that with planar Au and Cu2S CEs, respectively.

15.
Chem Asian J ; 8(10): 2483-8, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23846962

RESUMO

The tremendous future energy demand and environmental concerns prompt the lasting search for new materials for low-cost and high-efficiency solar cells. SnS, as a low-cost, earth-abundant, and environmentally friendly material with proper band gap and absorption coefficient, has received attention as a potential candidate for solar absorber, but it is still under-developed due to insufficient conversion efficiency. Fabricating SnS nanostructured films for solar cell design could be effective to boost photovoltaic performance and pave the way for applications in photovoltaics. Herein, a facile surfactant-free solution-based approach has been developed to prepare monolithic SnS nanostructured films directly on tin foil substrate. The morphologies of nanostructured films could be tuned from well-defined orthorhombic SnS nanobelt arrays to nanorods, nanosheets, or nanoflakes by simply changing the ratio of used solvents. The photoelectric response and electronic transportation properties of SnS nanobelts were investigated by fabricating single-nanobelt-based nanodevices. The SnS nanobelt exhibited a fast and reliable photoresponse even at illumination intensity as weak as 0.103 mW cm(-2). The measurements on SnS FET devices also indicated that the synthesized SnS nanobelts demonstrated a hole mobility as high as 12.33 cm(2) V(-1) s(-1). These results reveal that the reported approach for preparing monolithic SnS nanostructured films could be useful to further develop SnS as an alternative material for low-cost solar cells and electronic devices.

16.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 44(6): 924-7, 2013 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-24490503

RESUMO

OBJECTIVE: To investigate the effects of retinoic acid (RA) on the expression of Cx43 and its gap junction intercellular communication function in testicular cancer cells. METHODS: Cultured testicular cancer cells I-10 were treated with different concentration of RA (2.5, 5.0,10.0 micromol/L). The expression of Cx43 in 1-10 cells was detected by Western blot, and the distribution and location of Cx43 on cellular membrane was studied with immunofluorescence assay. Parachute assay was used to detect the function of gap junction intercellular communication composed of Cx43 in 1-10 cells. RESULTS: RA (2.5, 5.0, 10.0 micromol/L) markedly increased the expression of Cx43 in I-10 cells, the enhancement ratios were 43.14% +/- 2.1%, 58.09% +/- 1.8%, 143.13% +/- 1.6%, respectively. The result of immunofluorescence assay showed that RA (2.5, 5.0, 10.0 micromol/L) obviously increased the level of Cx43 located on the cellular membrane of I-10 cells. The result of parachute assay demonstrated that RA (2.5,5.0,10.0 gmol/L) could enhance the intercellular dye coupling through gap junction, the enhancement ratios were 26.1% +/- 2.3%, 63.3% +/- 1.6%, 140.5% +/- 3.4%, respectively. CONCLUSION: RA could enhance the gap junction intercellular communication by increasing the expression of Cx43 in I-10 cells.


Assuntos
Comunicação Celular/fisiologia , Conexina 43/fisiologia , Neoplasias Testiculares/metabolismo , Tretinoína/farmacologia , Conexina 43/genética , Junções Comunicantes/fisiologia , Humanos , Masculino , Neoplasias Testiculares/patologia , Células Tumorais Cultivadas
17.
Zhonghua Gan Zang Bing Za Zhi ; 17(6): 451-4, 2009 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-19567026

RESUMO

OBJECTIVES: To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1beta. METHODS: The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expression and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen I were detected by the quantitative immunocytochemical assay and ELISA. RESULTS: Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-1beta (P < 0.05). Synthesis and secretion of Type I collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1beta was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu. CONCLUSION: Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type I collagen, possibly via the repression of the JNK signal transduction.


Assuntos
Colágeno Tipo I/metabolismo , Células Estreladas do Fígado/efeitos dos fármacos , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Lactatos/farmacologia , Cirrose Hepática/prevenção & controle , Transdução de Sinais/efeitos dos fármacos , Animais , Western Blotting , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Regulação para Baixo , Células Estreladas do Fígado/metabolismo , Imuno-Histoquímica , Interleucina-1beta/farmacologia , Lactatos/administração & dosagem , Cirrose Hepática/etiologia , Masculino , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Ratos , Ratos Wistar
18.
Nan Fang Yi Ke Da Xue Xue Bao ; 29(5): 914-7, 921, 2009 May.
Artigo em Chinês | MEDLINE | ID: mdl-19460707

RESUMO

OBJECTIVE: To investigate the inhibitory effect of danshensu on c-Jun N-terminal kinase (JNK) and nuclear factor-kappa B (NF-kappaB) signaling in activated rat hepatic stellate cells (HSCs) and explore the mechanisms of danshensu for inhibiting hepatic fibrosis. METHODS: MTT colorimetric assay was used to detect the proliferation of rat HSCs treated with danshensu, and the apoptosis of the cells was analyzed with Annexin- V-FITC/PI and AO/EB staining. The expressions of P-IkappaB-alpha, NF-kappaBP65 and JNK in HSCs stimulated by IL-1beta with subsequent danshensu treatment were observed by Western blotting. Type III collagen in the medium of HSCs was detected by ELISA and immunocytochemistry. RESULTS AND CONCLUSIONS: Danshensu inhibited the activation and proliferation of HSCs, and increased the apoptotic rate of HSCs and reduced the synthesis and secretion of type III collagen. Danshensu showed obvious inhibitory effect on JNK and P-IkappaB-alpha phosphorylation and NF-kappaBP65 expression in HSCs stimulated by IL-1beta. The mechanism of the actions of dansgensu may be mediated by inhibition of JNK and NF-kappaB signal transduction.


Assuntos
Células Estreladas do Fígado/metabolismo , Interleucina-1beta/farmacologia , Lactatos/farmacologia , MAP Quinase Quinase 4/metabolismo , Transdução de Sinais , Fator de Transcrição RelA/metabolismo , Animais , Regulação para Baixo , Células Estreladas do Fígado/citologia , Masculino , Ratos , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos
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