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1.
Biosens Bioelectron ; 154: 112068, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32056963

RESUMO

Here we report the development of a high throughput, all-solution phase, and isothermal detection system for African Swine Fever Virus (ASFV). CRISPR-Cas12a programmed with a CRISPR RNA (crRNA) is used to detect ASFV target DNA. Upon ASFV DNA binding, the Cas12a/crRNA/ASFV DNA complex becomes activated and degrades a fluorescent single stranded DNA (ssDNA) reporter present in the assay. We combine this powerful CRISPR-Cas assay with a fluorescence-based point-of-care (POC) system for rapid and accurate virus detection. Without nucleic acid amplification, a detection limit of 1 pM is achieved within 2 h. In addition, the ternary Cas12a/crRNA/ASFV DNA complex is highly stable at physiological temperature and continues to cleave the ssDNA reporter even after 24 h of incubation, resulting in an improved detection limit of 100 fM. We show that this system is very specific and can differentiate nucleic acid targets with closely matched sequences. The high sensitivity and selectivity of our system enables the detection of ASFV in femtomolar range. Importantly, this system features a disposable cartridge and a sensitive custom designed fluorometer, enabling compact and simple ASFV detection, intended for low resource settings.

2.
Thorac Cancer ; 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31989777

RESUMO

BACKGROUND: The ProCore 25-gauge needle is a novel specifically designed puncture needle for endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), which may improve the puncture efficiency of the procedure while ensuring the diagnostic rate. The aim of the present study was to evaluate the diagnostic accuracy, mRNA yield, and complication rate of 25-gauge needles compared to those of 22-gauge needles in the evaluation of mediastinal and hilar lymphadenopathy. METHODS: A total of 39 patients undergoing EBUS-TBNA at our institution were evaluated. All the procedures were performed by an experienced endoscopist formally trained in interventional pulmonology. Both the traditional 22-gauge and ProCore 25-gauge needles were used at each lymph node station. For all specimens obtained via EBUS-TBNA, 50 µL was used to extract mRNA and detect the RNA concentration, whereas the other part was sent to the pathological evaluation. χ2 test and t-test were performed to determine the differences between the two types of the needles. A P-value of <0.05 was considered significant. RESULTS: A total of 88 lymph nodes were punctured by the two needles separately. The diagnostic accuracy for each puncture between the two needles did not show any significant difference (P > 0.05). No serious procedure-related complications were reported. In addition, the mRNA concentration did not differ between the two types of needles (P > 0.05). CONCLUSION: The ProCore 25-gauge needle gained a similar diagnostic yield with less puncture time and frequency compared with the 22-gauge needle.

3.
Int J Environ Health Res ; 30(1): 38-48, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30714826

RESUMO

Exposure to the heavy metal cadmium has adverse effects on human health, including DNA methylation. This study aimed to investigate the effects of cadmium on liver and kidney functions and Klotho gene methylation and to explore the relationship of methylation level with indicators of liver and kidney functions. Graphite furnace atomic absorption spectrometry was conducted to determine urinary cadmium, and an automatic biochemical analyzer was used to detect indices of liver and kidney functions. PCR pyrosequencing was performed to detect the methylation rate of Klotho. One-way ANOVA was adopted to compare the differences between groups, and the linear correlation to variables was analyzed. Cadmium exposure was negatively correlated with albumin level (r=-0.143, p=0.021) and positively correlated with urinary ß2-microglobulin level (r=0.229, p<0.001). However, the methylation levels of Klotho gene was decreased and increased by low and high doses of cadmium exposure, respectively. And Klothomethylation levels were negatively correlated with albumin levels and positively correlated with ß2-microglobulin levels.In this study, cadmium exposure affects liver and kidney functions as well as Klotho methylation levels, but the effect on Klotho methylation levels is not linear. Klotho methylation levels also influence liver and kidney functions.

4.
Wei Sheng Yan Jiu ; 48(6): 869-875, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31875807

RESUMO

OBJECTIVE: To analyze the prevalence and variation of hypotension in Chinese adults residents in 2002 and 2010-2012. METHODS: Data of the blood pressure measurements from 2002 and 2010-2012 China National Nutrition and Health Survey(CNNHS), a multistage stratified cluster random sampling survey was conducted in 2002, and the sample size was 147 488, 64 869 males, 82 619 females, 50 123 urban residents, 97 365 rural residents, 71 078 northern residents and 76 410 southern residents. A multi-stage stratified and population-proportional cluster random sampling method was used in 2010-2012, and the sample size of was 120 427, 52 499 males, 67 928 females, 60 214 urban residents, 60 213 rural residents, 53 863 northern residents and 66 564 southern residents. The population data published by the National Bureau of Statistics in 2009 was used as a standard population of the data result for 2002 and 2010-2012. Complex sampling weighting method was adopted in data analyses. The prevalence of hypotension and 95%CI in different populations were calculated. Chi-square test was used to compare the rates, multi-factor analysis was conducted by using SURVEYLOGISTIC model regression. RESULTS: In 2002, the prevalence rate of hypotension in Chinese adults was 3. 0%, 1. 9% in males, 4. 1% in females, 2. 7% in urban residents, 3. 3% in rural residents, 2. 8% in northern residents and 3. 2% in southern residents. The prevalence rate of hypotension was the highest in 18-44 age group(4. 3%). Among people with different body mass index(BMI), the prevalence of hypotension was higher in low weight group(7. 0%). From 2010 to 2012, the prevalence rate of hypotension in Chinese adults was 2. 1%, 1. 1% in males, 3. 2% in females, 1. 9% in urban residents, 2. 2% in rural residents, 1. 8% in northern residents and 2. 3%in southern residents. The prevalence rate of hypotension was the highest in 18-44 age group(3. 1%). Among people with different BMI, the prevalence of hypotension was higher in low weight group(7. 4%). The total prevalence of hypotension in adult residents in China from 2010 to 2012 was lower than that in 2002, and the difference was statistically significant(χ~2= 624. 3, P <0. 0001). In 2002 and 2010-2012, the prevalence of hypotension in adult residents of China decreased with the increase of age, and the prevalence of hypotension in men and women also decreased with the increase of age. Women were higher than men, the lower age group was higher than the higher age group, and the lower weight group was higher than the normal population. The differences were statistically significant. Multivariate Logistic regression analysis also showed that lower age group, women and people with low weight were more prone to develop hypotension. CONCLUSION: In 2002 and 2010-2012, the total prevalence of hypotension in adult residents of China, and that of hypotension in both men and women decreased with the increase of age. Women were higher than men, the lower age group was higher than the higher age group, and the lower weight group was higher than the normal population. 18-44 age group, women and low weight group are more likely to have low blood pressure. Compared with 2002, the total prevalence of hypotension in Chinese adult residents showed a decreasing trend from 2010 to 2012.


Assuntos
Hipotensão , População Rural , Adulto , Grupo com Ancestrais do Continente Asiático , China/epidemiologia , Feminino , Humanos , Hipotensão/epidemiologia , Masculino , Prevalência
5.
Wei Sheng Yan Jiu ; 48(6): 898-963, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31875812

RESUMO

OBJECTIVE: To obtain hemoglobin values in different regions of China is of great guiding value for judging anemia in children in different regions. METHODS: A total of 13 951 children aged 0-2 years old(female 6759, male 7192, urban area 6866, rural area 7085) in the 2010-2013 National Nutrition and Health Survey was analyzed the characteristics of hemoglobin spatial differentiation. SAS was used to analyze the data of children's hemoglobin result. Geographic Information System(GIS)spatial analysis method was used to form the spatial distribution map of hemoglobin mean values at 55 monitoring points, and inverse distance weighted spatial interpolation(IDW)method was used to obtain the spatial distribution map of children's hemoglobin in China. RESULTS: Based on the data of 13 951 children aged 0-2 years in the National Nutrition and Health Survey, it was found that the average hemoglobin value of Chinese children ranged from 112. 02 to 139. 03 g/L. The average hemoglobin value of Yi 'an County in Heilongjiang Province(139. 04 ± 1. 22 g/L) and Kangle County in Gansu Province(132. 43 ± 0. 82 g/L) was the highest, followed by Shanxi, Yunnan, Anhui, Qinghai and Fujian Provinces(124. 03-128. 72 g/L). The result of spatial interpolation of hemoglobin for children aged 0-2 in China showed that the hemoglobin of children aged0-2 in China showed spatial differences, forming inland high value areas with Heilongjiang, Gansu-Qinghai, Shanxi and Yunnan as the core, Anhui-Jiangsu-ZhejiangFujian coastal high value areas. Low-value areas of hemoglobin were formed in eastern Sichuan-Chongqing-western Hubei-southern Shaanxi, Inner Mongolia-Shanxi-Shaanxi, Beijing-Hebei, Jilin-Liaoning, southern Guangdong and Hainan. There was no significant correlation between the spatial distribution of hemoglobin and altitude in children aged 0-2 years in China in 2013. CONCLUSION: The spatial distribution of hemoglobin in children aged 0-2 years in China has significant spatial difference characteristics between different regions, with local higher value region and lower value region distribution, which has no significant correlation with altitude.


Assuntos
Hemoglobinas , Pequim , Pré-Escolar , China , Coleta de Dados , Feminino , Humanos , Lactente , Recém-Nascido
6.
Wei Sheng Yan Jiu ; 48(6): 902-906, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31875813

RESUMO

OBJECTIVE: To estimate the early initiation of breastfeeding status of Chinese children aged less than 24 months and its influence factors. METHODS: Data of children aged less than 24 months old was collected from Chinese Nutrition and Health Surveillance in 2013. Subjects were selected form 55 survey sites of mainland China by the multilevel stratified equal proportional cluster random sampling and the final effective sample size was 14 825. Caregivers of children completed a feeding and health questionnaire, which included questions on whether the children were put to the breast within one hour of birth, influence factors and the initiation time of breastfeeding. Prevalence of early initiation of breastfeeding, influence factors and time composition ratio of initiation of breastfeeding were calculated. RESULTS: The prevalence of early initiation of breastfeeding of Chinese children under 2 years old in 2013 was 26. 4%, among which big cities, small and medium-sized cities, ordinary rural areas, povertystricken rural areas were 30. 1%、24. 1%、23. 2% and 36. 9%, respectively. Caesarean section was the most important factor affecting early breastfeeding of infants in big cities, small and medium-sized cities and ordinary rural areas, accounting for 40. 7%, 51. 5%and 33. 8%, respectively. The main influencing factor in poor rural areas was lack of early breast-feeding knowledge, accounting for 44. 6%. The prevalence of infants initiated breastfeeding 2 ± 23 hours after birth was 31. 0% and the prevalence of infants initiated breastfeeding ≥24 hours after birth was 35. 3%. In big cities, the prevalence of the two periods were 31. 2% and 33. 4%. In small and medium-sized cities, the prevalence were35. 4% and 34. 4%. In ordinary rural areas, the prevalence were 26. 9% and 42. 3%. In poverty-stricken rural areas, the prevalence were comparatively low, which were 29. 5%and 23. 5%, respectively. CONCLUSION: The prevalence of early initiation of breastfeeding among infant aged less than 24 months is low in China. Caesarean section and lack of related knowledge were the main influencing factors in urban and rural areas respectively. Certain percentage of infants have severely delayed breast contact with their mothers and start breastfeeding.


Assuntos
Aleitamento Materno , Cesárea , Pré-Escolar , China , Cidades , Feminino , Humanos , Lactente , Recém-Nascido , Gravidez , Prevalência , População Rural
7.
Wei Sheng Yan Jiu ; 48(6): 913-918, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31875815

RESUMO

OBJECTIVE: To describe the hypertension awareness, treatment and control among adults in China in 2010-2012. METHODS: Data was from the China Nutrition and Health Surveillance in 2010-2012. A stratified multistage cluster sampling was used from 31 provinces, autonomous regions, and municipalities and selected 150 districts/counties. The sample was adults aged 18 and over selected through the method of Probability Proportion to Size(PPS). The mercury sphygmomanometer was used to measure the blood pressure. Each person had three measurements. The questionnaire was used to collect the information of hypertension awareness and control. RESULTS: The sample participants were 120 428 in 2010-2012. Among adults with hypertension, 46. 5%were aware of their hypertension, 41. 1% reported taking hypertension medicine in the last two weeks, and 13. 8% controlled the blood pressure. It was higher for hypertension awareness, treatment and control in women(49. 5%, 44. 2% and 14. 6%) than those of men(43. 0%, 37. 4% and 12. 9%). The three rates were increased with age. The prevalence of control was 33. 6% among the treated hypertensive individuals. It was higher for men and the rate was decreased with age. It was higher for hypertension awareness, treatment and control among hypertension and control among treated hypertensive participates in urban area(52. 7%, 47. 9%, 17. 9% and 37. 3%) than those in rural area(39. 5%, 33. 4%, 9. 2% and 27. 6%). The rates of overall, gender, and age groups were decreased in large city, medium and small city, general county, and poor county. CONCLUSION: The prevalence of hypertension awareness, treatment and control among hypertension and control among treated hypertensive participates among Chinese adults was increased in 2010-2012. But the rates were still keep a low level. The region and age differences need pay attention.


Assuntos
Hipertensão , Adolescente , Adulto , Anti-Hipertensivos , Pressão Sanguínea , Determinação da Pressão Arterial , China , Cidades , Estudos Transversais , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Masculino , Prevalência , Adulto Jovem
8.
Indoor Air ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31755597

RESUMO

Stunting adversely affects physical and mental outcomes of children. It has not been examined whether household air pollution from solid fuel combustion is a risk factor for stunting in children. In a total of 41,439 children aged 6-17 across China, height was measured using a unified protocol. Multivariable linear regression models and logistic regression models were used to assess the associations of solid fuel use for cooking/heating with stunting in children. Adjusted for covariates, cooking/heating with solid fuel was significantly associated with a lower z-score for height for age and sex (ß = -0.21 [-0.32 to -0.09] and -0.17 [-0.31 to -0.03], respectively) and an increased risk of stunting with an estimated ORs of 1.34 [1.07~1.68] and 1.37 [1.02~1.83], respectively. The risk of stunting associated with solid fuel use was statistically significant in high-age children. And the effect was greater on girls than on boys, though the difference was not statistically significant. Our study suggested that Chinese children living in households using solid fuel had a significantly higher risk of stunting than those living in households using cleaner fuel.

9.
Wei Sheng Yan Jiu ; 48(4): 531-536, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31601333

RESUMO

OBJECTIVE: To examine the association between alcohol consumption and metabolic syndrome(MS) among Chinese men and women aged 18 years and older. METHODS: The 2010᾿012 China National Nutrition and Health Survey(CNNHS) used multi-stage stratified cluster randomization sampling method to select study participants. Basic information, health-related behaviors and diseases history were collected by questionnaire. Drink information was collected by food frequency questionnaire. Metabolic syndrome was defined according to the updated NCEP ATP III criteria. A total of 102 591 participants aged 18 years and older were included in the analysis. Logistic regression model was performed to examine the association between alcohol consumption and MS. Restricted cubic spline(RCS) was performed to explore the dose response relation of alcohol intake and MS. RESULTS: The drink rate was 33. 66% in Chinese adults aged 18 years and older. Compared with non-drinkers, men who consumed⇿0 g/time had a lower MS prevalence(OR=0. 77, 95%CI 0. 66-0. 90). There was a linear dose response association between alcohol intake and MS among men but not among women. In men, there was no association between drinking type and MS. The OR of MS were 0. 91(95%CI 0. 81-1. 03), 0. 90(95%CI 0. 75-1. 08), 0. 85(95%CI 0. 54-1. 36), and 0. 96(95%CI 0. 84-1. 10) among men who consumed liquor, beer, wine, and mixed liquor. Compared with non-drinkers, women who drink liquor or mixed liquor had a decrease MS prevalence. The OR were 0. 74(95%CI 0. 56-0. 98) and 0. 78(95%CI 0. 66-0. 91). The association between drinking frequency and MS had no statistical significance and the P-trend was 0. 11 in men and 0. 31 in women. CONCLUSION: There is a linear dose response association between alcohol intake and MS among men but not in women. Men consumed >20 g/time have an increased MS prevalence.


Assuntos
Consumo de Bebidas Alcoólicas/epidemiologia , Síndrome Metabólica/epidemiologia , Adolescente , Adulto , Bebidas Alcoólicas , China/epidemiologia , Feminino , Humanos , Masculino , Vinho
10.
Int J Environ Health Res ; : 1-14, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31621395

RESUMO

Natural environment is an important factor affecting longevity. Soil, water and hair samples from Bama were assayed to investigate the effects of elements on the regional longevity. The concentrations of Cd, Co and Mg in soil and Co in drinking water were significantly higher in longevity area than those in non-longevity area (p < 0.05). The concentrations of K, Mo, Na, Pb and V in soil, Mg, Na, Fe, Li and Mn in drinking water and I, K, Mg, Mn, Na and Sr in hair were significantly lower in longevity area than those in non-longevity area (p < 0.05). Mg, Mn, Na and Sr in hair were affected by soil and drinking water. Our results indicate that adequate concentration of Mg in soil might benefit longevity, excessive concentrations of Na in soil, Mg, Mn and Na in drinking water and Mg, Mn, Na and Sr in hair might reduce lifespan of local residents.

11.
J Ultrasound Med ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31463979

RESUMO

OBJECTIVES: Early fetal echocardiography is becoming increasing common during the nuchal translucency scan period. The aim of this meta-analysis was to assess the accuracy of first-trimester fetal echocardiography in diagnosing congenital heart defects (CHDs). METHODS: The databases of PubMed, Embase, the Cochrane Library, and the Cumulative Index to Nursing and Allied Health Literature were systematically searched for the candidate articles, and the references of included studies were also examined. We recorded the characteristics of the included studies and assessed the quality of each study by the Quality Assessment of Diagnostic Accuracy Studies tool. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were calculated with Meta-Disc version 1.4 software (Ramón y Cajal Hospital, Madrid, Spain). We also evaluated the publication bias by using Stata version 12.0 software (StataCorp, College Station, TX). RESULTS: This meta-analysis included 18 studies with 26,201 fetal hearts. The overall pooled sensitivity, specificity, PLR, and NLR were 0.750, 0.999, 392.95, and 0.277, respectively. The DOR and AUC were 1736.0 and 0.9331. The pooled sensitivity, specificity, PLR, NLR, DOR, and AUC for major CHDs were 0.838, 1.000, 725.69, 0.203, 5084.8, and 0.9617. CONCLUSIONS: First-trimester fetal echocardiography had high value in diagnosing CHDs, especially major CHDs.

12.
J Cell Biochem ; 120(12): 19496-19508, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31297881

RESUMO

Pancreatic cancer (Pa) is a malignant tumor of the digestive tract with high degree of malignancy, this study aimed to obtain the hub genes in the tumorigenesis of Pa. Microarray datasets GSE15471, GSE16515, and GSE62452 were downloaded from Gene Expression Omnibus (GEO) database, GEO2R was conducted to screen the differentially expressed genes (DEGs), and functional enrichment analyses were carried out by Database for Annotation, Visualization and Integrated Discovery (DAVID). The protein-protein interaction (PPI) network was constructed with the Search Tool for the Retrieval of Interacting Genes (STRING), and the hub genes were identified by Cytoscape. Totally 205 DEGs were identified, consisting of 51 downregulated genes and 154 upregulated genes enriched in Gene Ontology terms including extracellular matrix (ECM) organization, collagen binding, cell adhesion, and pathways associated with ECM-receptor interaction, focal adhesion, and protein digestion. Two modules in the PPI were chosen and biological process analyses showed that the module genes were mainly enriched in ECM and cell adhesion. Twenty-four hub genes were confirmed, the survival analyses from the cBioPortal online platform revealed that topoisomerase (DNA) II α (TOP2A), periostin (POSTN), plasminogen activator, urokinase (PLAU), and versican (VCAN) may be involved in the carcinogenesis and progression of Pa, and the receiver-operating characteristic curves indicated their diagnostic value for Pa. Among them, TOP2A, POSTN, and PLAU have been previously reported as biomarkers for Pa, and far too little attention has been paid to VCAN. Analysis from R2 online platform showed that Pa patients with high VCAN expression were more sensitive to gemcitabine than those with low level, suggesting that VCAN may be an indicator to guide the use of the chemotherapeutic drug. In vitro experiments also showed that the sensitivity of the VCAN siRNA group to gemcitabine was lower than that of the control group. In conclusion, this study discerned hub genes and pathways related to the development of Pa, and VCAN was identified as a novel biomarker for the diagnose and therapy of Pa.

13.
Wei Sheng Yan Jiu ; 48(2): 200-207, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-31133095

RESUMO

OBJECTIVE: To explore the status of nutrition and associated factors among elderly in China. METHODS: Based the 2010-2012 China National Nutrition and Health Survey(CNNHS), a total of 34 373 participants aged 60 years and older were included in the analysis. People without weight, height, education level, family income level, drinking, smoking status and lack of physical activity information were excluded. The survey adopted a multi-stage stratified cluster random sampling method proportional to the population. Dietary data were collected by the 24-hour-dietary for 3 days questionnaire. Multivariable logistic regression model was performed to examine the association between sociodemographic characteristics and dietary intake with status of nutrition. RESULTS: The 34 373 subjects aged 60 years and older had a 6. 2% low body weight, 31. 8% overweight and 11. 5% obesity. The prevalent of low body weight of elderly women(6. 1%) was higher than that of elderly men(1. 4%). Women(47. 1%)had a higher overweight/obesity prevalence than men(39. 3%)(OR=1. 19, 95% CI 1. 10-1. 28). Participants living in rural areas had a higher prevalence of low body weight(OR=1. 41, 95% CI 1. 07-1. 86) and a lower overweight/obesity prevalence than urban residents(OR=0. 72, 95% CI 0. 61-0. 86). Education levels was negatively associated with low body weight(P<0. 05), but positively associated with overweight/obesity prevalence(P<0. 05). Physical activity levels were inversely related with low body weightand overweight/obesity(P<0. 05). High intake of wheat was associated with a decreased low body weight and an increased overweight/obesity prevalence(P<0. 05). The prevalence of overweight/obesity was negatively related with high intake of rice and organ meat(P<0. 05). CONCLUSION: Sex, region, education level, household income, smoking status, physical activity level and dietary intake were associated with the status of nutrition among elderly in China.


Assuntos
Grupo com Ancestrais do Continente Asiático/estatística & dados numéricos , Obesidade/epidemiologia , Sobrepeso/epidemiologia , População Rural/estatística & dados numéricos , População Urbana/estatística & dados numéricos , Idoso , Índice de Massa Corporal , Peso Corporal , China/epidemiologia , Estudos Transversais , Registros de Dieta , Exercício , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estado Nutricional , Prevalência , Fatores Sexuais , Fatores Socioeconômicos
14.
Psychol Health Med ; 24(10): 1277-1286, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31131632

RESUMO

Few studies have investigated the sex-related associations between serum uric acid and depression. This study aimed to explore the associations between serum uric acid and depression stratified by sex among middle-aged and elderly Chinese participants. Using the baseline data of the China health and retirement longitudinal study (CHARLS), a total of 10,522 participants aged 45 years and older were included in the final analysis. Depression symptoms were measured with the 10-term Center for Epidemiologic Studies Depression Scale (CES-D). A multivariable logistic regression model was performed to examine the associations between serum uric acid levels and depression, and the results are presented using odds ratios (ORs) and 95% confidence intervals (CIs). The prevalence of depression was 38% among middle-aged and elderly participants in China. In men, participants in the highest serum uric acid quartile had a decreased prevalence of depression relative to those in the lowest quartile (OR: 0.57; 95% CI: 0.41-0.81) after adjustment for potential confounders. However, there was no significant association between serum uric acid and depression among women. Participants with hyperuricemia were had no higher prevalence of depression relative to participants with normal serum uric acid level. Negative association between uric acid and depression was found in men, but not in women.

15.
Nutrients ; 11(4)2019 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-31013668

RESUMO

Undernutrition during early life may lead to obesity in adulthood. This study was conducted to examine the relationship between famine exposure during early life and the risk of abdominal obesity in adulthood. A total of 18,984 and 16,594 adults were surveyed in 2002 and 2010-2012 in two nationally representative cross-sectional surveys, namely China Nutrition and Health Survey, respectively. The risk of abdominal obesity was evaluated for participants born during 1956-1961 and compared with that of participants born during 1962-1964. The overall prevalence of abdominal obesity in adulthood showed a positive association with famine exposure during early life. The odds ratios of famine exposure were 1.31 (1.19-1.44) and 1.28 (1.17-1.40) in 2002 during fetal life and infancy and 1.09 (1.00-1.19) in 2012 during fetal life, respectively. The relationships between famine exposure and abdominal obesity across the famine exposure groups were distinct among females and those who lived in urban areas and were physical inactive (p < 0.05). Exposure to famine during early life was associated with increased risks of abdominal obesity in adulthood, which was partially alleviated by healthy lifestyle factors (e.g., physical activity).


Assuntos
Obesidade Abdominal/epidemiologia , Obesidade Abdominal/etiologia , Inanição , Idoso , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Inquéritos Nutricionais , Estado Nutricional , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Fatores de Risco
16.
Medicine (Baltimore) ; 98(8): e14525, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30813158

RESUMO

BACKGROUND: Conflicting results have been reported on the prognostic significance of serum uric acid (SUA) in patients with acute heart failure (AHF). This meta-analysis aimed to determine the prognostic significance of SUA level in patients with AHF. METHODS: We made a comprehensive literature search in Pubmed and Embase databases from inception to April 6, 2018. All available observational studies or post hoc analysis of randomized controlled trial that evaluated the prognostic value of SUA level in patients with AHF were eligible. Outcome of interests were all-cause mortality and the combined endpoint of death or readmission. Prognostic values of SUA level were summarized as higher vs lower SUA category or per 1 mg/ml SUA rise. RESULTS: Ten studies involving 12,854 AHF patients were identified and analyzed. AHF patients with the highest SUA level had an increased risk of all-cause mortality (risk ratio [RR] 1.43; 95% confidence intervals [CI] 1.31-1.56) and combined endpoint of death or readmission (RR 1.68; 95% CI 1.33-2.13) after adjusting potential variables. In addition, per 1 mg/ml SUA rise significantly increased by 11% and 12% higher risk all-cause mortality and combined endpoint of death or readmission, respectively. A leave out 1 study sensitivity analysis confirmed the reliability of the pooling effect sizes. CONCLUSION: This meta-analysis indicates that elevated SUA level independently predicts all-cause mortality and the combined endpoint of death or readmission in AHF patients. Measurement of SUA level may improve risk stratification of adverse outcomes in these patients.


Assuntos
Biomarcadores/sangue , Insuficiência Cardíaca/sangue , Hiperuricemia/complicações , Ácido Úrico/sangue , Idoso , Idoso de 80 Anos ou mais , Feminino , Insuficiência Cardíaca/mortalidade , Humanos , Masculino , Pessoa de Meia-Idade , Readmissão do Paciente/estatística & dados numéricos , Prognóstico , Fatores de Risco
17.
Am J Psychiatry ; 176(3): 217-227, 2019 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-30818990

RESUMO

OBJECTIVE: Tourette's syndrome is polygenic and highly heritable. Genome-wide association study (GWAS) approaches are useful for interrogating the genetic architecture and determinants of Tourette's syndrome and other tic disorders. The authors conducted a GWAS meta-analysis and probed aggregated Tourette's syndrome polygenic risk to test whether Tourette's and related tic disorders have an underlying shared genetic etiology and whether Tourette's polygenic risk scores correlate with worst-ever tic severity and may represent a potential predictor of disease severity. METHODS: GWAS meta-analysis, gene-based association, and genetic enrichment analyses were conducted in 4,819 Tourette's syndrome case subjects and 9,488 control subjects. Replication of top loci was conducted in an independent population-based sample (706 case subjects, 6,068 control subjects). Relationships between Tourette's polygenic risk scores (PRSs), other tic disorders, ascertainment, and tic severity were examined. RESULTS: GWAS and gene-based analyses identified one genome-wide significant locus within FLT3 on chromosome 13, rs2504235, although this association was not replicated in the population-based sample. Genetic variants spanning evolutionarily conserved regions significantly explained 92.4% of Tourette's syndrome heritability. Tourette's-associated genes were significantly preferentially expressed in dorsolateral prefrontal cortex. Tourette's PRS significantly predicted both Tourette's syndrome and tic spectrum disorders status in the population-based sample. Tourette's PRS also significantly correlated with worst-ever tic severity and was higher in case subjects with a family history of tics than in simplex case subjects. CONCLUSIONS: Modulation of gene expression through noncoding variants, particularly within cortico-striatal circuits, is implicated as a fundamental mechanism in Tourette's syndrome pathogenesis. At a genetic level, tic disorders represent a continuous spectrum of disease, supporting the unification of Tourette's syndrome and other tic disorders in future diagnostic schemata. Tourette's PRSs derived from sufficiently large samples may be useful in the future for predicting conversion of transient tics to chronic tic disorders, as well as tic persistence and lifetime tic severity.


Assuntos
Transtornos de Tique/genética , Síndrome de Tourette/genética , Estudos de Casos e Controles , Predisposição Genética para Doença/genética , Estudo de Associação Genômica Ampla , Humanos , Herança Multifatorial/genética , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco , Índice de Gravidade de Doença , Tirosina Quinase 3 Semelhante a fms/genética
18.
Eur J Nutr ; 2019 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-30854593

RESUMO

PURPOSE: Accurate data on water and beverage intakes are essential for assessing hydration adequacy and setting proper guidelines. The objective of this study is to identify the patterns and sociodemographic determinants of water intake and to assess the intake adequacy for children in China. METHODS: The study team recruited 41,439 children aged 6-17 years using a multi-stage cluster random sampling method. Daily water and beverage intakes were investigated with the standard questionnaires and measuring containers in face-to-face interviews. Each participant was assigned an adjustment weight to obtain a nationally representative sample. Sociodemographic factors influencing water intake were identified using multi-variable regressions. Water intake adequacy was evaluated by comparing with the recommended water intake (RWI). RESULTS: The mean ± standard deviation of total water intake (TWI) was 1603 ± 731 mL/day for boys and 1487 ± 661 mL/day for girls. Plain water, food moisture, and other beverages contributed 51%, 20%, and 29% of the TWI. Multi-variable analyses showed that TWI of children increased with age, in urban areas and day schools, and with parents' economic and educational levels. The majority (82%) of children had TWI not meeting the corresponding RWI, and the percentage increased with age except for 14-17-year-old boys. CONCLUSIONS: Plain water is still the major source of daily water intake by children in China. Unfortunately, the majority of children do not have sufficient water intake, which warrants future actions and guidelines targeting adequate hydration.

19.
Biol Psychiatry ; 85(4): 298-304, 2019 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-30424865

RESUMO

BACKGROUND: Tourette syndrome (TS) has a well-established genetic background, but its genetic architecture remains largely unknown. The authors investigated the role of polygenic risk scores (PRSs) derived from a TS genome-wide association study in relation to the occurrence of tics and associated traits in a general population cohort. METHODS: Using the most recent TS genome-wide association study (n = 4819 cases; n = 9488 controls) as the discovery sample, PRSs were calculated in Avon Longitudinal Study of Parents and Children participants (n = 8941). Regression analyses were used to assess whether PRS predicted the presence and chronicity of tics, and symptom severity of obsessive-compulsive disorder, attention-deficit/hyperactivity disorder, and autism spectrum disorder in Avon Longitudinal Study of Parents and Children participants. RESULTS: Following correction for multiple testing, the PRS significantly predicted the presence (R2 = .48%, p empirical = .01, Q = .04) but not the chronicity (R2 = .16%, p empirical = .07, Q = .14) of tics in the Avon Longitudinal Study of Parents and Children cohort; it did not predict the severity of obsessive-compulsive disorder (R2 = .11%, p empirical = .11, Q = .15), attention-deficit/hyperactivity disorder (R2 = .09%, p empirical = .19, Q = .21), or autism spectrum disorder (R2 = .12%, p empirical = .09, Q = .14). CONCLUSIONS: The authors found a significant polygenic component of tics occurring in a general population cohort based on PRS derived from a genome-wide association study of individuals with a TS diagnosis. This finding supports the notion that tics along a spectrum from nonclinical to clinical symptom levels share a similar genetic background.


Assuntos
Estudo de Associação Genômica Ampla , Herança Multifatorial/genética , Transtornos de Tique/genética , Síndrome de Tourette/genética , Adolescente , Transtorno do Deficit de Atenção com Hiperatividade/genética , Transtorno do Espectro Autista/genética , Estudos de Casos e Controles , Criança , Pré-Escolar , Estudos de Coortes , Feminino , Genótipo , Humanos , Lactente , Masculino , Transtorno Obsessivo-Compulsivo/genética , Fenótipo , Índice de Gravidade de Doença
20.
Am J Med Genet B Neuropsychiatr Genet ; 180(6): 351-364, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30456828

RESUMO

Obsessive-compulsive disorder (OCD) is a highly heritable complex phenotype that demonstrates sex differences in age of onset and clinical presentation, suggesting a possible sex difference in underlying genetic architecture. We present the first genome-wide characterization of the sex-specific genetic architecture of OCD, utilizing the largest set of OCD cases and controls available from the Psychiatric Genomics Consortium. We assessed evidence for several mechanisms that may contribute to sex differences including a sex-dependent liability threshold, the presence of individual sex-specific risk variants on the autosomes and the X chromosome, and sex-specific pleiotropic effects. Furthermore, we tested the hypothesis that genetic heterogeneity between the sexes may obscure associations in a sex-combined genome-wide association study. We observed a strong genetic correlation between male and female OCD and no evidence for a sex-dependent liability threshold model, suggesting that sex-combined analysis does not suffer from widespread loss of power because of genetic heterogeneity between the sexes. While we did not detect any significant sex-specific genome-wide single nucleotide polymorphisms (SNP) associations, we did identify two significant gene-based associations in females: GRID2 and GRP135, which showed no association in males. We observed that the SNPs with sexually differentiated effects showed an enrichment of regulatory variants influencing expression of genes in brain and immune tissues. These findings suggest that future studies with larger sample sizes hold great promise for the identification of sex-specific genetic risk factors for OCD.

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