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1.
ACS Appl Mater Interfaces ; 12(21): 24370-24379, 2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-32368896

RESUMO

Easy and rapid continuous large-scale industrial production of transparent visualized cutaneous wound healing dressing from natural polymers is very worth studying in medical natural polymer materials and multifunction gauze dressing design fields. In this work, superclear, porous cellulose membranes (CMs) with chitosan-coated nanofibers were fabricated using a simple, one-step electrostatic spinning technology and evaluated as potential wound dressings. First, the pure CMs were dissolved by a simple physical method, and then, the membranes were regenerated in an acidic coagulation bath by the casting method. The chitosan solution was polarized into nanofibers and formed a continuous fiber mat on CMs because of the charge repulsion between molecules. The prepared chitosan-coated CMs (CM-CS) were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, differential scanning calorimetry, tensile tests, and so forth. The results indicated that CM-CS showed high wettability, hydrophilicity, and gas permeability, in addition to excellent light transmittance and mechanical compliance. Cell cytotoxicity and morphology assay and antibacterial activity against Escherichia coli and Staphylococcus aureus were also studied. They exhibited good biocompatibility and antibacterial activity of CM-CS. Moreover, evaluation of an in vivo wound healing model in mice revealed that CM-CS had a good effect in promoting wound healing. This work provided an easy and rapid continuous large-scale industrial design strategy for natural bioresource-based wound dressing materials, which could act as potential wound dressings for clinical use.

2.
Nanoscale ; 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-32195496

RESUMO

AgO nanoparticles were successfully integrated into NiCo2O4 nanosheets for enhanced electrochemical catalysis ability and stability in the oxygen evolution reaction (OER). The chrysanthemum-like NiCo2O4/AgO composites mounted on nickel foam (NF) were synthesized by a hydrothermal-calcination method. AgO upgraded the ratio of Co3+/Co2+ and thus regulated the intrinsic activity of the species. The highly hierarchical structure of NiCo2O4/AgO composed of 0D AgO nanoparticles, 1D NiCo2O4 needles, 2D NiCo2O4 nanosheets, and 3D chrysanthemum-like bundles grown on NF bestowed the high surface area and mesoporous structure for the easy evolution of O2. The Ni atoms in NiCo2O4 originating in situ from NF in the process of AgO formation produced an integrated electrode of the active component of NiCo2O4 bound on NF with a superb highway for charge transfer. AgO significantly tuned the structure and physicochemical properties of NiCo2O4. As a result, NiCo2O4/AgO/NF exhibited excellent OER performance with an overpotential of 232 mV to obtain a current density of 10 mAcm-2 in an alkaline electrolyte, and the catalyst showed a small loss of the initial catalyst activity for 50 h and over 5000 cycles. This study provides a pathway for developing high-performance OER electrocatalysts.

3.
Biomater Sci ; 8(7): 1875-1884, 2020 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-32010912

RESUMO

Theranostic nanosystems are emerging as a promising approach for controlled drug delivery, diagnosis and multimodal therapeutics. Herein, a multifunctional theranostic nanoplatform is reported for photothermal-chemo combination therapy functioned with magnetic and thermal imaging. Hyaluronic acid (HA) coated Fe3O4@polydopamine nanoparticles equipped with redox-sensitive disulfide linkers have been subsequently deposited with an anticancer drug, doxorubicin (DOX) (termed as FPCH-DOX NPs). These nanocomposites possess an average diameter of 120 nm, a saturation magnetization of 28.5 emu g-1, DOX loading capacity of 7.13% and a transverse relaxation rate of 171.76 mM-1 s-1. The drug release could be triggered by pH, glutathione (GSH) concentration and light irradiation. Prussian blue staining and confocal microscopy demonstrate that these nanoplatforms have improved biocompatibility and cellular uptake in CD44-positive HeLa cell lines rather than in CD44-negative NIH 3T3 normal cell lines. In vitro evaluations demonstrate that the combination therapy of FPCH-DOX NPs lowers the cell viability to 16.2%, less than that of individual chemotherapy (55.3%) or PTT (52.1%). In vivo MRI indicates that the tumor accumulation of FPCH-DOX NPs provides enhanced MRI contrast, and in vivo thermal imaging verified their localized photothermal conversion effect in tumor tissues. Importantly, FPCH-DOX NPs present remarkable anti-tumor efficacy by photothermal-chemo combination therapy. H&E and Ki67 staining tests show obvious necrosis and weak cell proliferation at the region of the tumor. Thus, FPCH-DOX NPs are promising multifunctional nanoplatforms for highly effective cancer theranostics.

4.
Nanotechnology ; 31(13): 135101, 2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-31783383

RESUMO

The independence of photodynamic or photothermal modality create difficulties in the success of tumor therapy. In this current study, a multifunctional nanotheranostic agent of PDE-Ce6-HA was developed for tumor targeted and MRI-guided photodynamic/photothermal combined therapy (PDT/PTT). For this purpose, the near-infrared-absorbing nanoparticles of prussian blue were coated with polydopamine and successively conjugated with chlorin e6 (Ce6) for reactive oxygen species (ROS) generation. The resultant nanoparticles, denoted as PDE-Ce6, were then modified with hyaluronic acid (HA) through electrostatic interaction to yield the final therapeutic agent of PDE-Ce6-HA NPs. PDE-Ce6-HA NPs not only exhibited high colloid stability, good biocompatibility and suitable transverse relaxation rate (0.54 mM-1 s-1), but also high photothermal conversion efficiency (40.4%) and excellent ROS generation efficiency under NIR light irradiation. The confocal microscopy images demonstrated a selective uptake of PDE-Ce6-HA by CD44 overexpressed HeLa cells via HA-mediated endocytosis. Meanwhile, in vitro anti-cancer evaluation verified the significant photodynamic and photothermal combined effects of PDE-Ce6-HA on cancer cells. Moreover, PDE-Ce6-HA led to an increase of T1-MRI contrast in tumor site. Furthermore, in vivo anti-tumor evaluation proved that the PDE-Ce6-HA under both 808 and 670 nm laser showed significantly high tumor growth inhibition effects compared with individual PTT or PDT. Hence, PDE-Ce6-HA is applicable in tumor targeted and MRI-guided photodynamic/photothermal combined treatment.

5.
Chem Commun (Camb) ; 55(100): 15117-15120, 2019 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-31782438

RESUMO

A nitrogen doped bio-carbon catalyst with high specific surface area and a hierarchical interconnected porous structure was fabricated by an in situ gas-foaming strategy from sodium alginate and ammonium chloride. The optimized catalyst displays a fabulous ORR activity, providing a facile approach for the mass production of metal-free bio-carbon catalysts in fuel cells and metal-air batteries.


Assuntos
Carbono/química , Fontes de Energia Elétrica , Gases/química , Nitrogênio/química , Ar , Alginatos/química , Cloreto de Amônio/química , Catálise , Porosidade , Zinco/química
6.
Adv Mater ; 31(52): e1904894, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31709622

RESUMO

Cerenkov radiation (CR) from radionuclides can act as a built-in light source for cancer theranostics, opening a new horizon in biomedical applications. However, considerably low tumor-targeting efficiency of existing radionuclides and radionuclide-based nanomedicines limits the efficacy of CR-induced theranostics (CRIT). It remains a challenge to precisely and efficiently supply CR energy to the tumor site. Here, a "missile-detonation" strategy is reported, in which a high dose of p-SCN-Bn-deferoxamine-porphyrin-PEG nanocomplex (Df-PPN) is first adminstered as a CR energy receiver/missile to passively target to tumor, and then a low dose of the 89 Zr-labeled Df-PPN is administrated as a CR energy donor/detonator, which can be visualized and quantified by Cerenkov energy transfer imaging, positron-emission tomography, and fluorescence imaging. Based on homologous properties, the colocalization of Df-PPN and 89 Zr-Df-PPN in the tumor site is maximized and efficient CR energy transfer is enabled, which maximizes the tumor-targeted CRIT efficacy in an optimal spatiotemporal setting while also reducing adverse off-target effects from CRIT. This precise and efficient CRIT strategy causes significant tumor vascular damage and inhibited tumor growth.


Assuntos
Fármacos Fotossensibilizantes/química , Nanomedicina Teranóstica , Animais , Linhagem Celular Tumoral , Desferroxamina/química , Transferência de Energia , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Nanoestruturas/química , Neoplasias/irrigação sanguínea , Neoplasias/diagnóstico , Neoplasias/tratamento farmacológico , Neovascularização Patológica , Estresse Oxidativo/efeitos dos fármacos , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Fármacos Fotossensibilizantes/uso terapêutico , Polietilenoglicóis/química , Porfirinas/química , Tomografia por Emissão de Pósitrons , Radioisótopos/química , Distribuição Tecidual , Transplante Heterólogo , Zircônio/química
7.
Nanomaterials (Basel) ; 9(9)2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31547339

RESUMO

Metal organic frameworks (MOFs) have been exploited for various applications in science and engineering due to the possibility of forming different mesoscopic frameworks and pore structures. To date, further development of MOFs for practical applications in areas such as energy storage and conversion have encountered tremendous challenge owing to the unitary porous structure (almost filled entirely with micropores) and conventional morphology (e.g., sphere, polyhedron, and rod shape). More recently, one-dimensional (1D) MOFs/nanofibers composites emerged as a new molecular system with highly engineered novel structures for tailored applications. In this mini-review, the recent progress in the development of MOFs-based 1D nanofibers via electrospinning will be elaborated. In particular, the promising applications and underlying molecular mechanism of electrospun MOF-derived carbon nanofibers are primarily focused and analyzed here. This review is instrumental in providing certain guiding principles for the preparation and structural analysis of MOFs/electrospun nanofibers (M-NFs) composites and electrospun MOF-derived nanomaterials.

8.
Chem Commun (Camb) ; 55(62): 9216, 2019 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-31329198

RESUMO

Correction for 'Vehicle-saving theranostic probes based on hydrophobic iron oxide nanoclusters using doxorubicin as a phase transfer agent for MRI and chemotherapy' by Yanbing Cao et al., Chem. Commun., 2019, DOI: .

9.
Chem Commun (Camb) ; 55(61): 9015-9018, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31290867
10.
J Cell Biochem ; 120(10): 17006-17014, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31131464

RESUMO

An ideal positron emission tomography (PET) tracer should be highly extractable by the tumor tissue or organ that contains low toxicity and can provide high-resolution images in vivo. In this work, the aim was to evaluate the application of Al18 F-labeled 1,4,7-triazacyclononane-1,4,7-triacetic acid containing sulfonamide group (18 F-Al-NOTA-SN) as a potential tumor-targeting signal-enhanced radioactive tracer in PET. SN as a tumor-targeting group was incorporated to NOTA to make a ligand. Subsequently, this ligand reacted with Na18 F and AlCl3 to produce a compound 18 F-Al-NOTA-SN. This compound was further characterized and its property in regard to cell cytotoxicity assay, microPET imaging, biodistribution, cell uptake assay, and tumor selectivity in vitro and in vivo, was also investigated. 18 F-Al-NOTA-SN possessed low cell cytotoxicity and uptake to COS-7 and 293T healthy cells and high cell cytotoxicity and uptake to MDA-MB-231, HepG2, and HeLa tumor cells in vitro. Moreover, 18 F-Al-NOTA-SN showed good tumor-targeting property and high PET signal enhancement of HeLa tumors, liver, and kidneys in mice, as well as the uptake ratios of tumor to blood and tumor to muscle, were 4.98 and 3.87, respectively. 18 F-Al-NOTA-SN can be accepted to be kidney and liver eliminated earlier and show a potential tumor-targeting signal-enhanced radioactive tracer in PET.

11.
Polymers (Basel) ; 11(5)2019 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-31064069

RESUMO

The sustained release of antimicrobial therapeutics for wound dressing has become an attractive design strategy for prolonging the timespan of wound dressings and for reducing the risk of chronic wound infection. Recently, cellulose-based membrane has become a preferred option of wound dressings for the treatment of burn wounds and skin ulcers. In this work, novel cellulose membrane incorporated with mesoporous silica particles (SBA-15) was developed as an antimicrobial wound dressing with desirable sustained release functionality for targeting persistent bacterial pathogens. Attributed to a coated layer of calcium carbonate (CaCO3), SBA-15 particles were free from corrosion in alkaline condition during the preparation of cellulose-based composite membranes. SEM, TEM and BET results showed that the morphology, specific surface area, pore size and pore volume of pristine SBA-15 were preserved after the incorporation of CaCO3-coated SBA-15 into the cellulose matrix, while the mesoporous structure of SBA-15 was significantly disrupted without the use of CaCO3 coating. The resultant composite membranes containing 30 wt% SBA-15 (denoted as CM-Ca2-SBA(30%)) achieved 3.6 wt% of antimicrobial drug loading. Interestingly, CM-Ca2-SBA(30%) demonstrated the sustained release property of chloramphenicol for 270 h, driven by a two-stage drug release processes of SBA-15/cellulose. The water vapor permeability (WVTR) and swelling properties of composite membranes were shown to have complied with the primary requirements of wound dressing. Antibacterial assays revealed that strong antibacterial activities (144 h) of the composite membranes against Staphylococcus aureus and Eschericia coli were achieved. All results displayed that the strategy of coating silica with CaCO3 helps to obtain cellulose-silica composite membranes with desirable sustained release profiles and strong antibacterial activities. The antibacterial SBA-15/cellulose composite membranes show potential for the application of wound dressing.

12.
Biomater Sci ; 7(7): 2996-3006, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31111139

RESUMO

Fabricating multifunctional theranostic nanoparticles is highly pursued but still challenging for effective cancer treatment. Herein is reported a new theranostic nanoagent as both an MRI and targeted chemo/photothermal therapeutic agent. Prussian blue nanoparticles (PB) were first decorated with polydopamine (PDA), then conjugated with polyethylene glycol (PEG) and folic acid (FA), and finally loaded with doxorubicin (DOX) (denoted as PB@PDA@PEG-FA-DOX). The nanoagent was estimated to have an average size of 40 nm with a DOX-loading capacity of 36%, photothermal conversion efficiency of 45.7% and a transverse relaxation rate of 0.366 mM-1 s-1. In vitro release investigations showed a dual-responsive release by a mild acid and near-infrared (NIR) laser irradiation. PB@PDA@PEG-FA illustrated negligible cytotoxicity against the HL-7702 cell line and 38.2% cell viability under NIR against the HeLa cell line. PB@PDA@PEG-FA-DOX exhibited 45.2% cell viability. In contrast, the cell viability of PB@PDA@PEG-FA-DOX was dramatically decreased to 18.4% under NIR. Exclusive of folic acid, PB@PDA@PEG-DOX demonstrated 40.5% cell viability. These results demonstrated the potential of the nanoagent for integrated photothermal therapy (PTT) and chemotherapy, also embracing the FA targeting effect. In vivo MRI confirmed the effective nanoparticle accumulation, while infrared thermal images revealed the dramatically increased temperature under NIR at a tumor site. In vivo combination treatment-induced tumors were nearly completely destroyed without significant body weight loss after 14 days. H&E and Ki67 staining indicated remarkable necrosis and weak cell proliferation in the tumor area. Histologic examination revealed a lower toxicity in the vital organs. Therefore, this combination of chemo/photothermal therapy could provide an efficient route for cancer treatment.


Assuntos
Ferrocianetos/química , Ácido Fólico/química , Indóis/química , Imagem por Ressonância Magnética/métodos , Terapia de Alvo Molecular , Nanopartículas/química , Fototerapia/métodos , Polímeros/química , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Doxorrubicina/química , Doxorrubicina/farmacologia , Portadores de Fármacos/química , Portadores de Fármacos/metabolismo , Portadores de Fármacos/farmacocinética , Células HeLa , Humanos , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Polietilenoglicóis/química , Distribuição Tecidual
13.
Adv Mater ; 30(13): e1704934, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29430735

RESUMO

Nanoengineering of cell membranes holds great potential to revolutionize tumor-targeted theranostics, owing to their innate biocompatibility and ability to escape from the immune and reticuloendothelial systems. However, tailoring and integrating cell membranes with drug and imaging agents into one versatile nanoparticle are still challenging. Here, multicompartment membrane-derived liposomes (MCLs) are developed by reassembling cancer cell membranes with Tween-80, and are used to conjugate 89 Zr via deferoxamine chelator and load tetrakis(4-carboxyphenyl) porphyrin for in vivo noninvasive quantitative tracing by positron emission tomography imaging and photodynamic therapy (PDT), respectively. Radiolabeled constructs, 89 Zr-Df-MCLs, demonstrate excellent radiochemical stability in vivo, target 4T1 tumors by the enhanced permeability and retention effect, and are retained long-term for efficient and effective PDT while clearing gradually from the reticuloendothelial system via hepatobiliary excretion. Toxicity evaluation confirms that the MCLs do not impose acute or chronic toxicity in intravenously injected mice. Additionally, 89 Zr-labeled MCLs can execute rapid and highly sensitive lymph node mapping, even for deep-seated sentinel lymph nodes. The as-developed cell membrane reassembling route to MCLs could be extended to other cell types, providing a versatile platform for disease theranostics by facilely and efficiently integrating various multifunctional agents.


Assuntos
Neoplasias , Animais , Linhagem Celular Tumoral , Membrana Celular , Lipossomos , Camundongos , Tomografia por Emissão de Pósitrons , Nanomedicina Teranóstica , Distribuição Tecidual , Zircônio
14.
Angew Chem Int Ed Engl ; 57(1): 218-222, 2018 01 02.
Artigo em Inglês | MEDLINE | ID: mdl-29092090

RESUMO

The benefits to intracellular drug delivery from nanomedicine have been limited by biological barriers and to some extent by targeting capability. We investigated a size-controlled, dual tumor-mitochondria-targeted theranostic nanoplatform (Porphyrin-PEG Nanocomplexes, PPNs). The maximum tumor accumulation (15.6 %ID g-1 , 72 h p.i.) and ideal tumor-to-muscle ratio (16.6, 72 h p.i.) was achieved using an optimized PPN particle size of approximately 10 nm, as measured by using PET imaging tracing. The stable coordination of PPNs with 177 Lu enables the integration of fluorescence imaging (FL) and photodynamic therapy (PDT) with positron emission tomography (PET) imaging and internal radiotherapy (RT). Furthermore, the efficient tumor and mitochondrial uptake of 177 Lu-PPNs greatly enhanced the efficacies of RT and/or PDT. This work developed a facile approach for the fabrication of tumor-targeted multi-modal nanotheranostic agents, which enables precision and radionuclide-based combination tumor therapy.


Assuntos
Lutécio/metabolismo , Mitocôndrias/metabolismo , Imagem Multimodal , Nanopartículas/química , Neoplasias/terapia , Polietilenoglicóis/química , Porfirinas/metabolismo , Radioisótopos/metabolismo , Sistemas de Liberação de Medicamentos/métodos , Humanos , Neoplasias/metabolismo , Neoplasias/radioterapia , Imagem Óptica , Fotoquimioterapia/métodos , Tomografia por Emissão de Pósitrons , Nanomedicina Teranóstica/métodos
15.
ACS Appl Mater Interfaces ; 9(36): 30253-30257, 2017 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-28692244

RESUMO

With the objective to achieve in-between reduction-responsive drug release, selenylsulfide bond was first explored as a reduction cleavable linkage, compared with the most commonly employed disulfide and diselenide bonds. The reductive nanogel, with a combination of superparamagnetic and acid responsiveness, was fabricated. The expected release profiles were testified. Cytotoxicity assays illustrated the remarkable inhibition to the growth of HeLa cells, in contrast, high tolerance to L02 cells. In vivo investigation exhibited the obvious shrinkage in tumor but a healthy appearance. Hematoxylin-eosin staining and histological examination revealed the lower toxicity. The complex nanogels would have appeared highly promising in cancer therapy.

16.
Carbohydr Polym ; 166: 320-328, 2017 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-28385239

RESUMO

To fabricate ideal wound dressings without involvement of chemical processes is attracting the interest in clinical requirements. Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings. The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM). In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated. Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose. Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane. Chitosan was tightly coated on cellulose membranes without blocking the pores. The water vapor transmission rate was determined and exhibited suitable for ideal wound dressings. Especially, the antibacterial evaluation revealed that the coating of chitosan significantly enhanced the growth inhibition of both S. aureus and E. coli. Therefore, the obtained membrane was considered as a potential candidate for wound dressing materials.


Assuntos
Bandagens , Celulose/química , Quitosana/química , Ácido Cítrico/química , Compostos Férricos/química , Escherichia coli , Staphylococcus aureus
17.
Carbohydr Polym ; 151: 640-648, 2016 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-27474609

RESUMO

Many efforts have been driven to decontaminate the drinking water, and the development of efficient adsorbents with the advantages of cost-effectiveness and operating convenience for the removal of Pb(2+) from water is a major challenge. This work was aimed to explore the possibility of using cellulose-based adsorbents for efficient adsorption of Pb(2+). The millimeter-scale magnetic cellulose-based nanocomposite beads were fabricated via an optimal extrusion dropping technology by blending cellulose with the carboxyl-functionalized magnetite nanoparticles and acid-activated bentonite in NaOH/urea aqueous solution, and then they had been tested to evaluate the effectiveness in the removal of Pb(2+) from water. The effects of contact time, initial heavy metal ion concentrations, adsorption isotherms and solution pH on the sorption behavior were studied. The thermodynamic parameters (ΔG, ΔH and ΔS) indicated that the adsorption processes were feasible, spontaneous, endothermic and mainly controlled by chemical mechanisms. The reusability of the adsorbent was also studied.


Assuntos
Bentonita/química , Celulose/química , Chumbo/química , Chumbo/isolamento & purificação , Imãs/química , Nanocompostos/química , Purificação da Água/métodos , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Microesferas , Temperatura , Água/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/isolamento & purificação
18.
Mater Sci Eng C Mater Biol Appl ; 65: 354-63, 2016 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-27157762

RESUMO

A superparamagnetic nanogel featured with a logic "and"-type pH/reduction combinational stimulated release mode was fabricated as a drug delivery system by virtue of parallel crosslinking. The disulfide bond and electrostatic interaction between thiolated alginate (SA-SH) and thiolated/aminated iron oxide nanoparticles (SH-MION-NH2) were employed to achieve the mechanism. The obtained DOX-loaded magnetic nanogel is 122.7±20.3nm in size with superparamagnetism. The combinational conditions of pH5.0/10mM glutathione (GSH) stimulated a significantly high accumulative release. However, either pH7.4/10mM (GSH) or pH5.0 alone induced much low release. This verified the typical logic "and"-type combinationally stimulated release mode. In vitro cytotoxicity tests clearly illustrated the effective selectivity of killing the human cervical cancer cells (HeLa) with IC50 of 1.01µg/mL and the human hepatoma cells (HepG2) with IC50 of 1.57µg/mL but significantly low cytotoxicity to the cercopithecus aethiops kidney cells (Vero). CLSM presented the internationalization of the nanogel into cytoplasm and nuclei with time. In vivo investigation revealed that the selective intratumoral accumulation and antitumor efficacy were considerably advantageous over free DOX whereas low systemic toxicity exhibited up-regulated security as compared to free DOX. Overall, the DOX-loaded magnetic nanogel with enhanced antitumor efficacy and down-regulated adverse effect was a promising nanoplatform for the clinical chemotherapy of malignancy.


Assuntos
Antibióticos Antineoplásicos/química , Doxorrubicina/química , Polietilenoglicóis/química , Polietilenoimina/química , Alginatos/química , Animais , Antibióticos Antineoplásicos/administração & dosagem , Antibióticos Antineoplásicos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Chlorocebus aethiops , Doxorrubicina/administração & dosagem , Doxorrubicina/farmacologia , Portadores de Fármacos/química , Ácido Glucurônico/química , Glutationa/química , Células HeLa , Células Hep G2 , Ácidos Hexurônicos/química , Humanos , Concentração de Íons de Hidrogênio , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/patologia , Magnetismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Microscopia Confocal , Nanogéis , Tamanho da Partícula , Espectroscopia de Infravermelho com Transformada de Fourier , Transplante Heterólogo , Células Vero
19.
Carbohydr Polym ; 143: 231-8, 2016 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-27083364

RESUMO

The objective of this study is to develop transparent porous nanodiamonds/cellulose nanocomposite membranes with controlled release of doxorubicin for potential applications as wound dressings, which were fabricated by tape casting method from dispersing carboxylated nanodiamonds and dissolving cellulose homogeneously in 7 wt% NaOH/12 wt% urea aqueous solution. By adjusting the carboxylated nanodiamonds content, various nanocomposite membranes were obtained. The structure and properties of these membranes have been investigated by light transmittance measurements, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), tensile tests, water loss analyses, etc. The drug loading and release was investigated using doxorubicin hydrochloride as a model drug. In vitro cytotoxicity assay of the membranes was also studied. This work presented a proof-of-concept utility of these membranes for loading and release of bioactive compounds to be employed as a candidate for wound dressing.


Assuntos
Antineoplásicos/química , Celulose/química , Doxorrubicina/química , Membranas Artificiais , Nanodiamantes/química , Antineoplásicos/administração & dosagem , Bandagens , Doxorrubicina/administração & dosagem , Portadores de Fármacos , Módulo de Elasticidade , Células HeLa , Humanos , Concentração de Íons de Hidrogênio , Nanocompostos/química , Nanocompostos/toxicidade , Nanodiamantes/toxicidade , Tamanho da Partícula , Porosidade , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman , Resistência à Tração , Difração de Raios X
20.
Adv Mater ; 28(2): 254-62, 2016 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-26568542

RESUMO

A pyropheophorbide-α-based building block (Ppa-PLGVRG-Van) can be used to construct self-aggregated superstructures in vivo for highly specific and sensitive diagnosis of bacterial infection by noninvasive photoacoustic tomography. This in vivo supramolecular chemistry approach opens a new avenue for efficient, rapid, and early-stage disease diagnosis with high sensitivity and specificity.


Assuntos
Infecções Bacterianas/diagnóstico , Clorofila/análogos & derivados , Meios de Contraste , Nanoestruturas , Técnicas Fotoacústicas/métodos , Tomografia/métodos , Vancomicina/análogos & derivados , Animais , Infecções Bacterianas/complicações , Infecções Bacterianas/fisiopatologia , Clorofila/síntese química , Clorofila/química , Meios de Contraste/síntese química , Meios de Contraste/química , Modelos Animais de Doenças , Escherichia coli , Gelatinases/química , Camundongos , Modelos Biológicos , Estrutura Molecular , Miosite/complicações , Miosite/diagnóstico , Miosite/fisiopatologia , Nanoestruturas/química , Imagens de Fantasmas , Proteus vulgaris , Staphylococcus aureus , Staphylococcus epidermidis , Vancomicina/síntese química , Vancomicina/química , Água/química
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