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1.
Ying Yong Sheng Tai Xue Bao ; 30(11): 3716-3724, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31833684

RESUMO

Soil samples from four vegetation mini-patches (Artemisia scoparia, Glycyrrhiza uralensis, Sophora alopecuroides, Astragalus melilotoides) in a desert steppe in central Ningxia were collected. Soil physico-chemical properties including soil particle-size distribution, organic matter, pH, EC, total N, total K, total P of three depths were measured. The fractal dimension of particle size distribution characteristics of soils derived from four different vegetation mini-patches and their correlations with soil physico-chemical properties were examined. The results showed that patch vege-tation distribution affected the distribution of soil particle size, with the A. melilotoides mini-patch being the highest (D=2.51) and G. uralensis mini-patch being the lowest (D=2.46). There were significant positive correlation between fractal dimensions and the contents of clay and silt, and nega-tive correlation between fractal dimensions and sand content. Fractal dimensions were positively correlated with pH value and EC, negatively correlated with the contents of soil organic matter and total N, and had no correlation with the contents of soil total K and total P. The patchy vegetation distribution had potential trends of salinization and degradation.


Assuntos
Fractais , Solo , China , Argila , Nutrientes , Tamanho da Partícula
2.
Chin Med J (Engl) ; 132(21): 2543-2549, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31652142

RESUMO

BACKGROUND: The effect of short-segment decompression/fusion versus long-segment decompression/fusion and osteotomy for Lenke-Silva type VI adult degenerative scoliosis (ADS) has not been clarified. This study aimed to compare the clinical and radiographic results of short-segment fusion vs. long-segment fusion and osteotomy for patients with Lenke-Silva type VI ADS. METHODS: Data of 28 patients who underwent spinal surgery for ADS from January 2012 to January 2014 in the General Hospital of Northern Theater Command were reviewed. Of the 28 patients, 12 received long-segment fusion and osteotomy and 16 received short-segment fusion. Radiographic imaging parameters and clinical outcomes, including the sagittal vertical axis (SVA), lumbar lordosis (LL) angle, pelvic tilt (PT), sacral slope (SS), the visual analog scale (VAS), Japanese Orthopedic Association (JOA), Oswestry disability index (ODI), and lumbar stiffness disability index (LSDI) scores, were recorded. The difference between groups was compared using the dependent t test or Chi-squared test. RESULTS: The Cobb and LL angles and SVA improved in both groups; however, PT and SS angles did not improve following short fusion. There were significant differences in the post-operative SVA (26.8 ±â€Š5.4 mm vs. 47.5 ±â€Š7.6 mm, t = -8.066, P < 0.001), PT (14.7 ±â€Š1.8° vs. 29.1 ±â€Š3.4°, t = -13.277, P < 0.001), and SS (39.8 ±â€Š7.2° vs. 26.1 ±â€Š3.3°, t = 6.175, P < 0.001) between the long and short fusion groups. All patients had improved ODI, JOA, and VAS scores post-operatively (all P < 0.001), with no significant difference between the groups (all P > 0.05). The post-operative LSDI score was 3.5 ±â€Š0.5 in the long fusion group, which was significantly higher than that of the short fusion group (1.4 ±â€Š0.7; P < 0.001). CONCLUSIONS: The clinical outcomes of patients with Lenke-Silva type VI ADS who underwent short-segment decompression/fusion were comparable to those of patients who underwent long-segment decompression/fusion and osteotomy despite poor correction of sagittal imbalance. Moreover, short-segment decompression/fusion showed a short operation time and reduced surgical trauma.

3.
Ying Yong Sheng Tai Xue Bao ; 30(9): 3057-3065, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31529881

RESUMO

Vegetation patch is one of the most basic characteristics of natural grazing grassland. To explore the effects of vegetation patch on soil microbial community, the changes of soil microbial biomass and community structure under four different vegetation patches in Ningxia desert steppe were quantified using phospholipid fatty acid (PLFA) analysis. The results showed that: 1) Soil microbial groups were abundant in vegetation patches, with the highest bacterial content, low fungal and actinomycete content, and the Gram-positive bacteria content being higher than that of Gram-negative bacteria in the patches of the four plant communities; 2) The total soil microbial biomass of Glycyrrhiza uralensis patch was significantly higher than that of Artemisia scoparia, Sophora alopecuroides, and Astragalus melilotoides patches; 3) Total PLFAs, Gram-positive bacteria, Gram-negative bacteria, fungi, anaerobic bacteria and fungi/bacteria were significantly positively correlated with soil organic C, and significantly negatively correlated with soil pH, indicating that soil organic C and pH were important factors affecting the growth and development of soil microorganisms in desert steppe.


Assuntos
Ecossistema , Microbiologia do Solo , Solo , Bactérias , China , Clima Desértico , Fungos , Pradaria
4.
Neural Regen Res ; 14(5): 841-849, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30688270

RESUMO

Epithelial-specific ETS-1 (ESE1), a member of the ETS transcription factor family, is widely expressed in multiple tissues and performs various functions in inflammation. During neuroinflammation, ESE1 promotes neuronal apoptosis; however, the expression and biological functions of ESE1 remain unclear after cerebral ischemia/reperfusion. We performed in vivo and in vitro experiments to explore the role of ESE1 in cerebral ischemic injury. A modified four vessel occlusion method was used in adult Sprague-Dawley rats. At 6, 12, 24, 48, and 72 hours after model induction, the hippocampus was collected for analysis. Western blot assays and immunohistochemistry showed that the expression of ESE1, phosphorylated p65 and active caspase-3 was significantly up-regulated after ischemia. Double immunofluorescence staining indicated that ESE1 and NeuN were mostly co-located in the hippocampus after ischemia. Furthermore, ESE1 was also co-expressed with active caspase-3. PC12 cells were stimulated with cobalt chloride (CoCl2) to establish a chemical hypoxia model. After ESE1 knockdown by siRNA for 6 hours, cell viability was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assays. The levels of ESE1, phosphorylated p65 and active caspase-3 were also remarkably increased in PC12 cells after CoCl2 stimulation. After ESE1 knockdown, PC12 cell viability was increased after hypoxia. siRNA knockdown of ESE1 decreased the level of p-p65 and active caspase-3 after CoCl2 stimulation. These data reveal that ESE1 levels are elevated in the hippocampus after cerebral ischemia/reperfusion injury. This may play a role in neuronal apoptosis via activation of the nuclear factor-κB pathway.

5.
Biochimie ; 152: 31-42, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29705132

RESUMO

A high failure rate of titanium implants in diabetic patients has been indicated in clinical evidences. Excessive oxidative stress at the bone-implant interface plays an important role in the impaired osteointegration under diabetic conditions. While the underlying mechanisms remain unknown and the targeted treatments are urgently needed. Ophiopogonin D (OP-D), isolated from Chinese herbal Radix Ophiopogon japonicus, is generally reported to be a potent antioxidant agent. In the present study, we hypothesized that OP-D exerted promotive effects on osteointegration against oxidative stress, and investigated the underlying mechanisms associated with alteration of Wnt/ß-catenin signaling pathway. Rabbit osteoblasts incubated on titanium alloy implant were co-cultured with normal serum (NS), diabetic serum (DS), DS + OP-D, DS + NAC (a potent ROS inhibitor) and DS + OP-D + Dkk1 (a Wnt inhibitor) for examinations of osteoblast behaviors. For in vivo study, titanium alloy implants were implanted into the femoral condyle defects on diabetic rabbits. Results demonstrated that diabetes-induced oxidative stress resulted in osteoblast dysfunctions and apoptotic injury at the bone-implant interface, concomitant with the inactivation of Wnt/ß-catenin signaling. Importantly, OP-D administration attenuated oxidative stress, directly reactivating Wnt/ß-catenin signaling. Osteoblast dysfunctions were thus reversed as evidenced by improved osteoblast adhesion, proliferation and differentiation, and ameliorated apoptotic injury, exerting similar effects to NAC treatment. In addition, the positive effects afforded by OP-D were confirmed by improved osteointegration and oetogenesis within the titanium alloy implants in vivo by Micro-CT and histological analyses. Furthermore, the pro-osteogenic effects of OP-D were almost completely abolished by the Wnt inhibitor Dkk1. These results demonstrated, for the first time, OP-D administration alleviated the damaged osteointegration of titanium alloy implants under diabetic conditions by means of inhibiting oxidative stress via a Wnt/ß-catenin-dependent mechanism. The OP-D administration would become a reliable treatment strategy for implant failure therapy in diabetics due to the optimal anti-oxidative and pro-osteogenic properties.


Assuntos
Ligas , Antioxidantes/farmacologia , Diabetes Mellitus Experimental/fisiopatologia , Osseointegração/efeitos dos fármacos , Próteses e Implantes , Espécies Reativas de Oxigênio/metabolismo , Saponinas/farmacologia , Espirostanos/farmacologia , Titânio , Via de Sinalização Wnt/efeitos dos fármacos , beta Catenina/metabolismo , Fosfatase Alcalina/metabolismo , Animais , Antioxidantes/metabolismo , Apoptose/efeitos dos fármacos , Regeneração Óssea , Interface Osso-Implante , Adesão Celular , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Diabetes Mellitus Experimental/metabolismo , Masculino , Osteoblastos/citologia , Osteoblastos/enzimologia , Estresse Oxidativo , Impressão Tridimensional , Coelhos , Regeneração/efeitos dos fármacos
6.
Biomater Sci ; 6(1): 225-238, 2017 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-29231215

RESUMO

Chitosan (CS)-based hydroxyapatite (HA) composites have emerged as a novel strategy for promoting bone regeneration. Here nanophase HA/CS composite coated porous titanium implants (nCT) were fabricated and their biological behavior under diabetic conditions was investigated. We proposed that the focal adhesion kinase (FAK)-mediated BMP-2/Smad pathway played a role in mediating the promotive effect of nCTs on osteoblast adhesion and differentiation under diabetes-induced high reactive oxygen species (ROS) condition. To confirm the hypothesis, rat osteoblasts on bare titanium implants (Ti) and nCT were subjected to normal serum (NS), diabetic serum (DS), DS + NAC (a potent ROS inhibitor) and DS + cytochalasin D (an actin polymerization inhibitor). In vivo on diabetic sheep implanted with Ti or nCT showed that diabetes-induced ROS overproduction impaired osteoblast adhesion, evidenced by immunostaining of F-actin and vinculin and morphological observation through inhibition of FAK phosphorylation, which contributed to suppressed BMP-2-dependent Smad1/5/8 phosphorylation. nCT substrate reactivated the FAK-BMP-2/Smad pathway, thus reversing osteoblast dysfunction, which exerted a similar effect to NAC treatment on Ti. These effects were further confirmed by improved osteointegration within nCT in diabetic sheep, evidenced by micro-CT and histological examinations. Our study demonstrated that reactivation of the FAK-BMP-2/Smad pathway was involved in improving osteoblast adhesion and differentiation by nano-HA/CS composite coating, potentially directing biomaterial modification and biofunctionalization under diabetic conditions.


Assuntos
Diferenciação Celular/fisiologia , Animais , Diferenciação Celular/genética , Quitosana/química , Durapatita/química , Osteoblastos/citologia , Fosforilação , Ratos , Espécies Reativas de Oxigênio/metabolismo , Ovinos , Titânio/química , Vinculina/química
7.
Chin J Integr Med ; 23(12): 948-955, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29079906

RESUMO

OBJECTIVE: To provide evidence-based recommendations for clinical application and provoke thoughts for future researchers by conducting a comprehensive summary and evaluation of the current evidence profile for the role of Chinese medicine (CM) in treating myocardial infarction (MI). METHODS: Online databases including PubMed, EMBase, Cochrane Library, Chinese National Knowledge Infrastructure (CNKI), Chinese Biomedical Medicine (CBM), VIP Journal Integration Platform, and Wanfang database were systematically searched for literatures on CM in treating MI. After screening, studies were categorized into 5 types, i.e. systematic review (SR), randomized controlled trial (RCT), observational study, case report and basic research. General information was abstracted, and the quality levels of these studies and their conclusions were summarized and assessed. RESULTS: A total of 452 studies including 10 SRs, 123 RCTs, 47 observational studies, 28 case reports, and 244 basic researches were selected. Clinical studies centered primarily on herbal decoction and mostly were not rigorously performed. High-quality studies were predominantly on Chinese patent medicines (CPMs) such as Danshen Injection (), Shenmai Injection (), Shengmai Injection () and Qishen Yiqi Dripping Pills (). The most frequently observed pattern of drug combination was decoction plus injection. Results of SRs and clinical studies showed that CM may reduce mortality, decrease risk of complication, reduce myocardial injury, improve cardiac function and inhibit ventricular remodeling. Findings from basic researches also supported the positive role of CM in reducing infarct size and myocardial injury, promoting angiogenesis, preventing ventricular remodeling and improving cardiac function. According to the current evidence body, CM has proven effects in the prevention and treatment of MI. It is also found that the effects of CPMs vary with indications. For instance, Shenmai Injection has been found to be especially effective for reducing the incidence of acute clinical events, while CPMs with qi-nourishing and bloodcirculating properties have been proven to be effective in inhibiting ventricular remodeling. High quality evidence supports the use of CM injection for acute MI and CPM for secondary prevention. Reports on adverse events and other safety outcomes associated with CM for MI are scarce. CONCLUSIONS: Sufficient evidence supported the use of CM as an adjuvant to Western medicine for preventing and treating MI. The choice of drug use varies with disease stage and treatment objective. However, the quality of the evidence body remains to be enhanced.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Medicina Baseada em Evidências , Infarto do Miocárdio/tratamento farmacológico , Animais , Formas de Dosagem , Humanos , Infarto do Miocárdio/prevenção & controle , Estudos Observacionais como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto
8.
Exp Ther Med ; 14(2): 1744-1748, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28810645

RESUMO

Assessing the biomechanical properties of trabecular bone is of major biological and clinical significance for the research of bone diseases, fractures and their treatments. Micro-finite element (µFE) models are becoming increasingly popular for investigating the biomechanical properties of trabecular bone. The shapes of µFE models typically include cube and cylinder. Whether there are differences between cubic and cylindrical µFE models has not yet been studied. In the present study, cubic and cylindrical µFE models of human vertebral trabecular bone were constructed. A 1% strain was prescribed to the model along the superior-inferior direction. E values were calculated from these models, and paired t-tests were performed to determine whether these were any differences between E values obtained from cubic and cylindrical models. The results demonstrated that there were no statistically significant differences in the E values between cubic and cylindrical models, and there were no significant differences in Von Mises stress distributions between the two models. These findings indicated that, to construct µFE models of vertebral trabecular bone, cubic or cylindrical models were both feasible. Choosing between the cubic or cylindrical µFE model is dependent upon the specific study design.

9.
BMC Genomics ; 18(1): 230, 2017 03 14.
Artigo em Inglês | MEDLINE | ID: mdl-28288583

RESUMO

BACKGROUND: Due to its variegated and colorful leaves, ornamental kale (Brassica oleracea L. var. acephala) has become a popular ornamental plant. In this study, we report the fine mapping and analysis of a candidate purple leaf gene using a backcross population and an F2 population derived from two parental lines: W1827 (with white leaves) and P1835 (with purple leaves). RESULTS: Genetic analysis indicated that the purple leaf trait is controlled by a single dominant gene, which we named BoPr. Using markers developed based on the reference genome '02-12', the BoPr gene was preliminarily mapped to a 280-kb interval of chromosome C09, with flanking markers M17 and BoID4714 at genetic distances of 4.3 cM and 1.5 cM, respectively. The recombination rate within this interval is almost 12 times higher than the usual level, which could be caused by assembly error for reference genome '02-12' at this interval. Primers were designed based on 'TO1000', another B. oleracea reference genome. Among the newly designed InDel markers, BRID485 and BRID490 were found to be the closest to BoPr, flanking the gene at genetic distances of 0.1 cM and 0.2 cM, respectively; the interval between the two markers is 44.8 kb (reference genome 'TO1000'). Seven annotated genes are located within the 44.8 kb genomic region, of which only Bo9g058630 shows high homology to AT5G42800 (dihydroflavonol reductase), which was identified as a candidate gene for BoPr. Blast analysis revealed that this 44.8 kb interval is located on an unanchored scaffold (Scaffold000035_P2) of '02-12', confirming the existence of assembly error at the interval between M17 and BoID4714 for reference genome '02-12'. CONCLUSIONS: This study identified a candidate gene for BoPr and lays a foundation for the cloning and functional analysis of this gene.


Assuntos
Brassica/genética , Mapeamento Cromossômico , Antocianinas/biossíntese , Cromossomos de Plantas , DNA de Plantas/isolamento & purificação , DNA de Plantas/metabolismo , Genoma de Planta , Mutação INDEL , Fenótipo , Folhas de Planta/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo
10.
Theor Appl Genet ; 129(8): 1625-37, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27206841

RESUMO

KEY MESSAGE: A novel allele-specific Rfo marker was developed and proved to be effective for MAS of Rfo gene in B. oleracea background and six Ogu-CMS fertility-restored interspecific hybrids were created for the first time. Ogura cytoplasmic male sterility (Ogu-CMS) has been extensively used for Brassica oleracea hybrid production. However, because of maternal inheritance, all the hybrids produced by CMS lines are male sterile and cannot be self-pollinated, which prohibits germplasm maintenance and innovation. This problem can be overcome by using the Ogu-CMS restorer line, but restorer material is absent in B. oleracea crops. Here, Rfo, a fertility-restored gene of Ogu-CMS, was transferred from rapeseed restorer lines into a Chinese kale Ogu-CMS line using interspecific hybridization combined with embryo rescue. Nine interspecific, triploid plant progenies were identified at morphological and ploidy level, with phenotypes intermediate between those of rapeseed and Chinese kale. Because the Rfo marker (Hu et al., Mol Breeding 22:663-674, 2008) cannot distinguish the Rfo and its homologies under a B. oleracea background, a novel allele-specific Rfo marker was developed based on the BLAST analysis of highly homologous Rfo sequences in B. oleracea. Screening using the novel Rfo marker found that six interspecific hybrids carrying Rfo were also fertile, although fertility varied during different flowering periods. Furthermore, BC1 offsprings with the Rfo gene were selected with the allele-specific Rfo marker and showed restored fertility. These results indicated that the novel allele-specific marker could be used for the MAS of Rfo gene in B. oleracea, and this study lays the foundation for the development of Ogu-CMS restorer material in cabbage and its related other subspecies.


Assuntos
Brassica/genética , Marcadores Genéticos , Hibridização Genética , Infertilidade das Plantas/genética , Alelos , Sequência de Bases , Brassica/fisiologia , DNA de Plantas/genética
11.
Spine J ; 16(10): e649-e650, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26928879
12.
Mod Rheumatol ; 25(2): 282-5, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25645283

RESUMO

OBJECTIVES: This study aimed to investigate the outcomes after using a combination of anterior-posterior approaches to treat ankylosing spondylitis (AS) complicated by a multiple-level cervical vertebral Chance fracture. METHODS: We retrospectively analyzed 11 patients with AS complicated by a multiple-level cervical vertebral Chance fracture. RESULTS: After surgery, the average follow-up period was 25 months (24-27 months). No injury to the spinal cord, nerve roots, or vessels was caused by screw insertion during the operation. The incisions for all the patients healed by primary intention. These fractures generally required 4-6 months (average, 4.5 months) to heal, and there was no loosening, pullout or collapse of the bone graft, loosening or breaking of the internal fixators, nonunion, or other complications. CONCLUSIONS: Therefore, management with a combination of anterior-posterior approaches is feasible for treating AS complicated by a multiple-level cervical vertebral Chance fractures.


Assuntos
Vértebras Cervicais/cirurgia , Fixação Interna de Fraturas/métodos , Fraturas da Coluna Vertebral/cirurgia , Fusão Vertebral/métodos , Espondilite Anquilosante/cirurgia , Adulto , Parafusos Ósseos , Vértebras Cervicais/diagnóstico por imagem , Vértebras Cervicais/lesões , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Estudos Retrospectivos , Fraturas da Coluna Vertebral/complicações , Fraturas da Coluna Vertebral/diagnóstico por imagem , Espondilite Anquilosante/complicações , Espondilite Anquilosante/diagnóstico por imagem , Resultado do Tratamento
13.
PLoS One ; 9(4): e94635, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24722354

RESUMO

BACKGROUND: ZFP580 is a novel C2H2 type zinc-finger transcription factor recently identified by our laboratory. We previously showed that ZFP580 may be involved in cell survival and growth. The aim of this study was to elucidate whether ZFP580 is involved in the cardioprotective effects of intermittent high-altitude (IHA) hypoxia against myocardial ischemia-reperfusion (I/R) injury. METHODS AND RESULTS: After rats were subjected to myocardial ischemia for 30 min followed by reperfusion, ZFP580 expression in the left ventricle was measured. ZFP580 protein expression was found to be up-regulated within 1 h and decreased at 2 h after reperfusion. Comparing normoxic and IHA hypoxia-adapted rats (5000 m, 6 h day-1, 6 weeks) following I/R injury (30 min ischemia and 2 h reperfusion), we found that adaptation to IHA hypoxia attenuated infarct size and plasma leakage of lactate dehydrogenase and creatine kinase-MB. In addition, ZFP580 expression in the myocardium was up-regulated by IHA hypoxia. Consistent with this result, ZFP580 expression was found to be significantly increased in cultured H9c2 myocardial cells in the hypoxic preconditioning group compared with those in the control group following simulated I/R injury (3 h simulated ischemic hypoxia and 2 h reoxygenation). To determine the role of ZFP580 in apoptosis, lentivirus-mediated gene transfection was performed in H9c2 cells 72 h prior to simulated I/R exposure. The results showed that ZFP580 overexpression significantly inhibited I/R-induced apoptosis and caspase-3 activation. H9c2 cells were pretreated with or without PD98059, an inhibitor of ERK1/2 phosphorylation, and Western blot results showed that PD98059 (10 µM) markedly suppressed I/R-induced up-regulation of ZFP580 expression. CONCLUSIONS: Our findings demonstrate that the cardioprotective effect of IHA hypoxia against I/R injury is mediated via ZFP580, a downstream target of ERK1/2 signaling with anti-apoptotic roles in myocardial cells.


Assuntos
Adaptação Fisiológica/fisiologia , Hipóxia/metabolismo , Isquemia Miocárdica/metabolismo , Traumatismo por Reperfusão Miocárdica/metabolismo , Fatores de Transcrição/metabolismo , Dedos de Zinco/fisiologia , Altitude , Animais , Apoptose/fisiologia , Creatina Quinase Forma MB/metabolismo , Ventrículos do Coração/metabolismo , L-Lactato Desidrogenase/metabolismo , Masculino , Miocárdio/metabolismo , Fosforilação , Ratos , Ratos Wistar , Regulação para Cima
14.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 30(5): 396-400, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25581930

RESUMO

OBJECTIVE: To elucidate whether ZFP580 is involved in the cardioprotective effects of intermittent hypobaric hypoxia (IHH) against myocardial ischemia/reperfusion (I/R) injury. METHODS: Thirty two male Wistar rats were randomly divided into 2 groups (n = 16): normoxia control group and IHH preconditioning group. Rats in IHH group were exposed in a hypobaric chamber (equivalent to an altitude of 5 000 m) for a 6 h period each day for 42 d. Plasma was collected and lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) were measured after 2 h of myocardial I/R injury. ZFP580 protein expression in myocardial tissue was assayed by Western blot. Other 8 rats in each group were used to evaluate I/R-induced cardiac infarction by TTC staining. Lentivirus-mediated gene transfection was performed in H9c2 cells 72 h prior to simulated ischemia/reperfusion (SI/R) exposure. The degree of cell apoptosis was determined by annexin V/7-AAD staining and flow cytometry analysis. RESULTS: Compared with normoxia control group, adaptation to IHH attenuated infarct size and plasma leakage of LDH and CK-MB. In addition, ZFP580 expression in the myocardium was up-regulated by IHH. The results of gene transfection showed that ZFP580 overexpression significantly inhibited cells apoptosis induced by SI/R. CONCLUSION: Our findings demonstrate that the cardioprotective effect of IHH against I/R injury is mediated via ZFP580, a novel transcription factor, with anti-apoptotic roles in myocardial cells.


Assuntos
Hipóxia/fisiopatologia , Traumatismo por Reperfusão Miocárdica/fisiopatologia , Fatores de Transcrição/metabolismo , Animais , Apoptose , Linhagem Celular , Creatina Quinase Forma MB/metabolismo , L-Lactato Desidrogenase/metabolismo , Masculino , Miocárdio/metabolismo , Miócitos Cardíacos/citologia , Ratos , Ratos Wistar
15.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 29(4): 326-30, 2013 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-24175554

RESUMO

OBJECTIVE: To explore the role of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4) in myocardial ischemia/reperfusion injury (MI/RI) by observing the dynamic expression changes at mRNA and protein levels early after myocardial ischemia/reperfusion (I/ R). METHODS: The Wistar rats were randomly divided into Sham and I/R group (n = 42), and killed according to different reperfusion time (1, 2, 4, 6, 12, 24 h and 7 d). Structural and morphous changes of myocytes were observed under optical microscope. The mRNA and protein levels of TLR2 and TLR4 were detected using real-time PCR (RT-PCR). Monocyte chemokine protein-1 (MCP-1) and interleukine-6 (IL-6) mRNA levels were measured by reverse transcriptase-polymerase chain reaction (rt-PCR). RESULTS: (1) With the extension of reperfusion time, the myocardial infarct size increased smoothly, and reached the plateau at 4 h, then stayed in the platform. After reperfusion for 7 d, the ventricular had been remodeled. (2) At the beginning of reperfusion, myocardial structure showed no significant change in Sham group, but had different degrees of injury in I/R group. In rats of the group reperfused for 7 d the left ventricular remodeling could be visible. (3) Compared to sham group,TIR2, TLR4, MCP-1, IL-6 mRNA level were increased in myocardium in I/R group. TLR2 and TLR4 both peaked at 4 h of reperfusion, IL6 peaked at 6 h, followed by a gradually decrease. TLR4 and IL-6 mRNA levels rose again at 7 d. MCP-1 level in I/R group remained fairly with sham group at the beginning of reperfusion, and markedly elevated at 7 d. CONCLUSION: Expression of TLRs mRNA in myocardium during early after myocardial ischemia/reperfusion increased rapidly and activated TLRs might play an important role in MI/RI through promoting the generation of inflammatory factors. At the late reperfusion, TLRs levels raise again and the expression of inflammatory factors increase once again, Those may probably affect the remodeling of ventricular, and injure myocardial structure and function.


Assuntos
Traumatismo por Reperfusão Miocárdica/metabolismo , Receptor 2 Toll-Like/metabolismo , Receptor 4 Toll-Like/metabolismo , Animais , Quimiocina CCL2/metabolismo , Modelos Animais de Doenças , Interleucina-6/metabolismo , Masculino , Ratos , Ratos Wistar
16.
World J Gastroenterol ; 19(37): 6258-64, 2013 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-24115824

RESUMO

AIM: To investigate the stress-induced apoptosis of natural killer (NK) cells and the changes in their killing activity in mouse livers. METHODS: A restraint stress model was established in mice. Flow cytometry was employed to measure the percentage of NK cells and the changes in their absolute number in mouse liver. The cytotoxicity of hepatic and splenic NK cells was assessed against YAC-1 target cells via a 4 h 51Cr-release assay. RESULTS: The restraint stress stimulation induced the apoptosis of NK cells in the liver and the spleen, which decreased the cell number. The number and percentage of NK cells in the spleen decreased. However, the number of NK cells in the liver decreased, whereas the percentage of NK cells was significantly increased. The apoptosis of NK cells increased gradually with prolonged stress time, and the macrophage-1 (Mac-1)(+) NK cells were more susceptible to apoptosis than Mac-1(-) NK cells. Large numbers of Mac-1(-) NK cells in the liver, which are more resistant to stress-induced apoptosis, were observed than the Mac-1(-) NK cells in the spleen. The stress stimulation diminished the killing activity of NK cells in the spleen was significantly decreased, but the retention of numerous Mac-1(-) NK cells in the liver maintained the killing ability. CONCLUSION: Significant stress-induced apoptosis was observed among Mac-1(+) NK cells, but not Mac-1(-) NK cells in the mouse liver. Stress stimulation markedly decreased the killing activity of NK cells in the spleen but remained unchanged in the liver.


Assuntos
Apoptose , Células Matadoras Naturais/patologia , Fígado/patologia , Restrição Física/psicologia , Baço/patologia , Estresse Psicológico/patologia , Animais , Biomarcadores/análise , Células Matadoras Naturais/imunologia , Fígado/imunologia , Antígeno de Macrófago 1/análise , Camundongos , Camundongos Endogâmicos C57BL , Baço/imunologia , Estresse Psicológico/imunologia
17.
Bioresour Technol ; 147: 29-36, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23985372

RESUMO

A potential commercial pretreatment for furfural residues (FRs) was investigated by using a combination of green liquor and hydrogen peroxide (GL-H2O2). The results showed that 56.2% of lignin removal was achieved when the sample was treated with 0.6 g H2O2/g-DS (dry substrate) and 6 mL GL/g-DS at 80 °C for 3 h. After 96 h hydrolysis with 18 FPU/g-cellulose for cellulase, 27 CBU/g-cellulose for ß-glucosidase, the glucose yield increased from 71.2% to 83.6%. Ethylenediaminetetraacetic acid was used to reduce the degradation of H2O2, the glucose yield increased to 90.4% after the addition of 1% (w/w). The untreated FRs could bind more easily to cellulase than pretreated FRs could. The structural changes on the surface of sample were characterized by X-ray photoelectron spectroscopy. The results indicated that the surface lignin could be effectively removed during pretreatment, thereby decreasing the enzyme-lignin binding activity. Moreover, the carbonyl from lignin plays an important role in cellulase binding.


Assuntos
Celulase/química , Furaldeído/química , Peróxido de Hidrogênio/química , Adsorção , Ácido Edético/química , Hidrólise , Espectroscopia Fotoeletrônica
18.
Zhongguo Zhen Jiu ; 33(12): 1127-30, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24617247

RESUMO

Based on the structure and function of acupoint and in association of the definition and principle of sensor, the acupoint is the sensitive element, being sensitive to the physical stimulation with acupuncture and moxibustion and sensitively responded to the disorders; the acupoint is the sensing element, transforming the changes of the acupoint information via the complicated internet conduction, integration and regulation, so as to generate the effects on organic body; the acupoint is the conversion element, transforming every irritation into the bioelectric signal or optical signal so that the organic body could recognize it. Therefore, the acupoint is regarded as the sensor of information in the organic body.


Assuntos
Pontos de Acupuntura , Fenômenos Eletrofisiológicos , Humanos , Meridianos
19.
ACS Appl Mater Interfaces ; 4(2): 665-71, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22264080

RESUMO

The paper describes for the first time the successful synthesis of Fe(2)O(3)/TiO(2) tube-like nanostructures, in which TiO(2) shell is of quasi-single crystalline characteristic and its thickness can be controlled through adjusting the added amount of aqueous Ti(SO(4))(2) solution. The characterization of samples obtained at different stages using transmission electron microscope indicates that the outer TiO(2) shell is changed gradually from amorphous and polycrystalline phase into quasi-single crystal under thermal actions through the Ostwald ripening process, accompanying the corrosion of the central parts of Fe(2)O(3) nanorods, and the formation of small particles separating each other, leading to the special core/shell nanorods. Furthermore, Fe(2)O(3)/TiO(2) tube-like nanostructures can be transformed into Fe(2)TiO(5) nanostructures after they are thermally treated at higher temperatures. Those nanostructures exhibit enhanced ethanol sensing properties with respect to the monocomponent. Our results imply that not only hollow nanostructures, but also a novel type of nanostructures can be fabricated by the present method for nanodevices.


Assuntos
Compostos Férricos/química , Nanoestruturas/química , Titânio/química , Gases/análise , Nanoestruturas/ultraestrutura , Temperatura , Água/química
20.
Eur Spine J ; 19(10): 1795-8, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20449612

RESUMO

Open-door laminoplasty is a commonly performed procedure for the treatment of multiple level cervical spinal stenosis. One complication of this procedure is closure of the hinge and subsequent restenosis. Twinfix suture anchor was used in laminoplasty to stabilize cervical canal expansion. 53 patients with multiple level cervical spinal stenosis underwent laminoplasty. A unilateral open-door technique was performed for the lesion level and the elevated lamina was fixed to the lateral mass using Twinfix suture anchors. Radiography, magnetic resonance imaging, and computed tomography (CT) scanning were used for imaging studies. The Japanese Orthopedic Association score was adopted to compare clinical outcome before and after surgery. None of 53 patients who had the door secured with Twinfix suture anchors had closure of the hinge. Additionally, the suture anchors maintained their position without loosening or "pull-outs" on postoperative follow-up radiographs. The Japanese Orthopedic Association score increased significantly from 8.5 ± 3.2 before surgery to 14.2 ± 1.36 at final follow-up. Postoperative radiography and CT scan demonstrated significantly increased anteroposterior diameter of the spinal canal. There were four short-term complications: two were small dural-tears which were repaired intraoperatively without further sequelae, and the other 2 were both epidural hematomas that required emergent return to the operating room for evacuation. There were no Twinfix suture anchor-related complications. This Twinfix suture anchor can provide a firm and secure anchor for elevated open laminae in laminoplasty.


Assuntos
Vértebras Cervicais/cirurgia , Laminectomia/instrumentação , Laminectomia/métodos , Estenose Espinal/cirurgia , Espondilose/cirurgia , Âncoras de Sutura/normas , Técnicas de Sutura/instrumentação , Idoso , Vértebras Cervicais/diagnóstico por imagem , Vértebras Cervicais/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Estudos Retrospectivos , Canal Vertebral/diagnóstico por imagem , Canal Vertebral/patologia , Canal Vertebral/cirurgia , Estenose Espinal/patologia , Estenose Espinal/fisiopatologia , Espondilose/patologia , Espondilose/fisiopatologia
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