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1.
Front Pharmacol ; 12: 741801, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34621173

RESUMO

Background: Hirudin has been widely used in the treatment of antifibrosis. Previous studies have shown that hirudin can effectively improve the clinical remission rate of chronic kidney disease. However, the mechanism of its renal protection has not been systematically investigated. Methods: In this study, the reliability of UUO-induced renal interstitial fibrosis was evaluated by histopathological verification. High-throughput transcriptome sequencing was used to elucidate the molecular mechanism of hirudin, differentially expressed mRNAs were identified, and their functions were analyzed by GO analysis and GSEA. In addition, the RNA-seq results were validated by in vitro and vivo experiments. Results: We found 322 identical differential expressed genes (IDEs) in the UUO hirudin-treated group compared with the sham group. Functional enrichment analysis indicated that cellular amino acid metabolic processes were the most obvious enrichment pathways in biological processes. In terms of molecular functional enrichment analysis, IDEs were mainly enriched in coenzyme binding, pyridoxal phosphate binding and other pathways. In addition, microbody is the most obvious pathway for cellular components. A total of 115 signaling pathways were enriched, and AMPK, JAK-STAT, and PI3K-Akt signaling pathways were the important signaling pathways enriched. We found that PI3K, p-Akt, and mTOR expression were significantly reduced by hirudin treatment. In particular, our results showed that hirudin could induce a decrease in the expression of autophagy-related proteins such as P62, LC3, Beclin-1 in TGF-ß1-induced NRK-52E cells. Conclusion: Our results suggest that hirudin may protect the kidney by ameliorating renal autophagy impairment through modulating the PI3K/Akt pathway.

2.
Drug Des Devel Ther ; 15: 4167-4175, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34629864

RESUMO

Background: Upadacitinib, a novel selective Janus kinase 1 (JAK1) inhibitor, has been recently approved by the US FDA for the treatment of adult patients with moderately to severely active rheumatoid arthritis (RA). An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative analysis of upadacitinib in beagle dog plasma was developed and validated. Methods: Upadacitinib and fedratinib (internal standard, IS) were extracted with ethyl acetate under alkaline condition and then separated and detected. The chromatographic column was Waters Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 µm), the mobile phase was acetonitrile and 0.1% formic acid in water with gradient elution procedure, and the flow rate was 0.40 mL/min. Under the positive ion mode, upadacitinib and IS were monitored by multiple reaction monitoring (MRM) as the following mass transition pairs: m/z 447.00 → 361.94 for upadacitinib and m/z 529.82 → 141.01 for IS. Results: In the concentration range of 1-500 ng/mL, upadacitinib had good linearity, and the lower limit of quantification (LLOQ) was 1 ng/mL. The RSD of the intra- and inter-day precision was less than 10.03%, and the RE of accuracy was -3.79% to 2.58%. The extraction recovery of upadacitinib was more than 80%, the matrix effect was around 100%, and upadacitinib was found to be stable. Conclusion: The novel optimized UPLC-MS/MS assay was an effective tool for the determination of upadacitinib and had been successfully applied to the pharmacokinetic study of upadacitinib in beagle dogs, and this method would also be used to study DDIs.

3.
Front Pharmacol ; 12: 715466, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34630093

RESUMO

Infarcted myocardium is predisposed to cause lethal ventricular arrhythmias that remain the main cause of death in patients suffering myocardial ischemia. Liver-derived fibroblast growth factor 21 (FGF21) is an endocrine regulator, which exerts metabolic actions by favoring glucose and lipids metabolism. Emerging evidence has shown a beneficial effect of FGF21 on cardiovascular diseases, but the role of FGF21 on ventricular arrhythmias following myocardial infarction (MI) in humans has never been addressed. This study was conducted to investigate the pharmacological effects of FGF21 on cardiomyocytes after MI in humans. Patients with arrhythmia in acute MI and healthy volunteers were enrolled in this study. Serum samples were collected from these subjects on day 1 and days 7-10 after the onset of MI for measuring FGF21 levels using ELISA. Here, we found that the serum level of FGF21 was significantly increased on day 1 after the onset of MI and it returned to normal on days 7-10, relative to the Control samples. In order to clarify the regulation of FGF21 on arrhythmia, two kinds of arrhythmia animal models were established in this study, including ischemic arrhythmia model (MI rat model) and nonischemic arrhythmia model (ouabain-induced guinea pig arrhythmia model). The results showed that the incidence and duration time of ischemic arrhythmias in rhbFGF21-treated MI rats were significantly reduced at different time point after MI compared with normal saline-treated MI rats. Moreover, the onset of the first ventricular arrhythmias was delayed and the numbers of VF and maintenance were attenuated by FGF21 compared to the rhbFGF21-untreated group in the ouabain model. Consistently, in vitro study also demonstrated that FGF21 administration was able to shorten action potential duration (APD) in hydrogen peroxide-treated AC16 cells. Mechanically, FGF21 can ameliorate the electrophysiological function of AC16 cells, which is characterized by rescuing the expression and dysfunction of cardiac sodium current (I Na) and inward rectifier potassium (I k1) in AC16 cells induced by hydrogen peroxide. Moreover, the restorative effect of FGF21 on NaV1.5 and Kir2.1 was eliminated when FGF receptors were inhibited. Collectively, FGF21 has the potential role of ameliorating transmembrane ion channels remodeling through the NaV1.5/Kir2.1 pathway by FGF receptors and thus reducing life-threatening postinfarcted arrhythmias, which provides new strategies for antiarrhythmic therapy in clinics.

4.
J Inflamm Res ; 14: 5023-5050, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34616171

RESUMO

Emerging data indicates that non-coding RNAs (ncRNAs) represent more than just "junk sequences" of the genome and have been found to be involved in multiple diseases by regulating various biological process, including the activation of inflammasomes. As an important aspect of innate immunity, inflammasomes are large immune multiprotein complexes that tightly regulate the production of pro-inflammatory cytokines and mediate pyroptosis; the activation of the inflammasomes is a vital biological process in inflammatory diseases. Recent studies have emphasized the function of ncRNAs in the fine control of inflammasomes activation either by directly targeting components of the inflammasomes or by controlling the activity of various factors that control the activation of inflammasomes; consequently, ncRNAs may represent potential therapeutic targets for inflammatory diseases. Understanding the precise role of ncRNAs in controlling the activation of inflammasomes will help us to design targeted therapies for multiple inflammatory diseases. In this review, we summarize the regulatory role and therapeutic potential of ncRNAs in the activation of inflammasomes by focusing on a range of inflammatory diseases, including microbial infection, sterile inflammatory diseases, and fibrosis-related diseases. Our goal is to provide new ideas and perspectives for future research.

5.
Open Life Sci ; 16(1): 1002-1009, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34616914

RESUMO

In this pilot study, we compared the dynamic changes of circulating immune cells between patients with hepatocellular carcinoma (HCC) who underwent radiofrequency ablation (RFA) and hepatectomy. Seventy-three patients were enrolled in this study. Flow cytometry assay was performed to determine the immune cells in the peripheral blood mononuclear cells (PBMCs) before treatment and on days 7, 14, and 28 after treatment. We found that in the RFA group, the circulating cluster of differentiation (CD)4+ cells, the CD4+/CD8+ ratio, and natural killer (NK) cells continued to increase, and the circulating CD8+ cells continued to decrease after the treatment. In contrast, in the surgery group, the circulating CD4+ cells and CD4+/CD8+ ratio decreased over the first seven postoperative days and then began to increase, and CD8+ cells decreased on the first 7 postoperative days and began to increase thereafter. The changes of immune cells in tumor tissues consisted of an increase in the number of CD4+ cells, CD8+ cells, CD3+ cells, and NK cells immediately after RFA. Our results show that postoperative immune function continued to improve after RFA, but after surgery, it decreased in the first week and started to improve thereafter. These findings are important for clinicians when selecting the appropriate therapy for HCC.

6.
Opt Express ; 29(15): 23421-23429, 2021 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-34614607

RESUMO

Nano-clusters and nano-particles (NPs) are attractive media for high-harmonic generation (HHG) since they combine the advantages of using atomic media (for the low average density) and bulk solid media (for the high local density). Recently, laser ablated plumes from metal nano-powders have been used as HHG media and it has been often assumed that the harmonics mainly come from the NPs in the plumes but not by the isolated atoms/ions. However, this assumption is yet to be fully justified. Here, we show that in fact both NPs and isolated monomers could dominate the harmonic spectrum, depending on which part of the plume is interacting with the driving laser. From the ablated plume of indium NPs, it is found that the harmonic spectra from the region where monomers dominate are distinctively different from the region where NPs dominate. Our results demonstrate that accurately capturing the contribution of NPs in HHG processes requires precise selection of the laser-plasma interaction region, a factor that had not been carefully considered in previous studies.

7.
Asian J Surg ; 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34629275

RESUMO

OBJECTIVE: To enhance students' understanding and imagination and improve teachers' teaching quality through the "Hand As Foot" teaching method. METHODS: Teachers explained the hierarchical structure of brain and the location of intracranial hematoma through the "Hand As Foot" teaching method. RESULTS: Through the "Hand As Foot" teaching method, students can deeply remember the hierarchical structure of brain and the location of intracranial hematoma. CONCLUSION: This novel and unique teaching method makes the interaction between teachers and students stronger and improves the teaching quality.

8.
Ultrason Sonochem ; 79: 105764, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34601447

RESUMO

This study evaluated efficacy of high-intensity ultrasound (HIU) on controlling or stimulating Staphylococcus aureus biofilm. Acoustic pressure distribution on the surface of glass slide cultivated S. aureus biofilm was first simulated as a standardized parameter to reflect sono-effect. When the power of HIU was 240 W with acoustic pressure of -1.38×105 Pa, a reasonably high clearance rate of S. aureus biofilm was achieved (96.02%). As an all-or-nothing technique, the HIU did not cause sublethal or injury of S. aureus but inactivate the cell directly. A further evaluation of HIU-induced stimulation of biofilm was conducted at a low power level (i.e. 60 W with acoustic pressure of -6.91×104 Pa). The low-power-long-duration HIU treatment promoted the formation of S. aureus biofilm and enhanced its resistance as proved by transcriptional changes of genes in S. aureus, including up-regulations of rbf, sigB, lrgA, icaA, icaD, and down-regulation of icaR. These results indicate that the choose of input power is determined during the HIU-based cleaning and processing. Otherwise, the growth of S. aureus and biofilm formation are stimulated when treats by an insufficiently high power of HIU.

9.
Chemosphere ; : 132575, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34656618

RESUMO

The environmental and dietary pesticide exposures can cause thyroid hormones (THs) disorders, which are associated with the high incidence of thyroid diseases worldwide. The structures of diphenyl ether pesticides and their metabolites are very similar to the structure of THs. Based on this, in silico molecular simulation approaches were used to predict, screen, evaluate and identify the binding interactions of 98 diphenyl ether structure pesticides and their metabolites (DEPMs) with 10 THs related proteins in the study. The research results indicated that these DEPMs such as fluoroglycofen (FOG), rafoxanide, diclofop, ethoxyfen and difenopenten were considered to have the greater potentials to interfere with the related proteins of THs biosynthesis, blood transport, receptor binding and metabolism. And FOG can interact with thyroid hormone receptor beta (TRß) to form non-bond interactions. Furthermore, the results of molecular dynamics simulations showed that there were strong and stable interactions between FOG and TRß. These results suggested that the herbicide FOG was likely to disturb THs nuclear receptor. And benzene rings and hydrophobic groups might be the characteristic chemical functional groups for DEPMs to disrupt TRß. The relevant results of this study can be used to provide references for environmental toxicology evaluation, food safety risk assessment, and formulation and revision of pesticides and their metabolites residue limits in agricultural products and food.

10.
Zhongguo Yi Liao Qi Xie Za Zhi ; 45(5): 573-579, 2021 Sep 30.
Artigo em Chinês | MEDLINE | ID: mdl-34628776

RESUMO

OBJECTIVE: To explore the feasibility of using the bidirectional local distance based medical similarity index (MSI) to evaluate automatic segmentation on medical images. METHODS: Taking the intermediate risk clinical target volume for nasopharyngeal carcinoma manually segmented by an experience radiation oncologist as region of interest, using Atlas-based and deep-learning-based methods to obtain automatic segmentation respectively, and calculated multiple MSI and Dice similarity coefficient (DSC) between manual segmentation and automatic segmentation. Then the difference between MSI and DSC was comparatively analyzed. RESULTS: DSC values for Atlas-based and deep-learning-based automatic segmentation were 0.73 and 0.84 respectively. MSI values for them varied between 0.29~0.78 and 0.44~0.91 under different inside-outside-level. CONCLUSIONS: It is feasible to use MSI to evaluate the results of automatic segmentation. By setting the penalty coefficient, it can reflect phenomena such as under-delineation and over-delineation, and improve the sensitivity of medical image contour similarity evaluation.


Assuntos
Planejamento da Radioterapia Assistida por Computador , Estudos de Viabilidade
11.
Kidney Dis (Basel) ; 7(5): 372-390, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34604344

RESUMO

Background: Transforming growth factor-ß (TGF-ß)/Smad signaling is the central mediator in renal fibrosis, yet its functional role in acute kidney injury (AKI) is not fully understood. Recent evidence showed that TGF-ß/Smad3 may be involved in the pathogenesis of AKI, but its functional role and mechanism of action in cisplatin-induced AKI are unclear. Objectives: Demonstrating that Smad3 may play certain roles in cisplatin nephropathy due to its potential effect on programmed cell death and inflammation. Methods: Here, we established a cisplatin-induced AKI mouse model with Smad3 knockout mice and created stable in vitro models with Smad3 knockdown tubular epithelial cells. In addition, we tested the potential of Smad3-targeted therapy using 2 in vivo protocols - lentivirus-mediated Smad3 silencing in vivo and use of naringenin, a monomer used in traditional Chinese medicine and a natural inhibitor of Smad3. Results: Disruption of Smad3 attenuated cisplatin-induced kidney injury, inflammation, and NADPH oxidase 4-dependent oxidative stress. We found that Smad3-targeted therapy protected against loss of renal function and alleviated apoptosis, RIPK-mediated necroptosis, renal inflammation, and oxidative stress in cisplatin nephropathy. Conclusions: These findings show that Smad3 promotes cisplatin-induced AKI and Smad3-targeted therapy protects against this pathological process. These findings have substantial clinical relevance, as they suggest a therapeutic target for AKI.

12.
Chirality ; 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34608664

RESUMO

In order to develop new type of chiral separation materials, in this study, 6-amino-6-deoxyamylose was used as chiral starting material with which 10 derivatives were synthesized. The amino group in 6-amino-6-deoxyamylose was selectively acylated and then the hydroxyl groups were carbamoylated yielding amylose 6-amido-6-deoxy-2,3-bis(phenylcarbamate)s, which were employed as chiral selectors (CSs) for chiral stationary phases of high-performance liquid chromatography. The resulted 6-amido-6-deoxyamyloses and amylose 6-amido-6-deoxy-2,3-bis(phenylcarbamate)s were characterized by IR, 1 H NMR, and elemental analysis. Enantioseparation evaluations indicated that most of the CSs demonstrated a moderate chiral recognition capability. The 6-nonphenyl (6-nonPh) CS of amylose 6-cyclohexylformamido-6-deoxy-2,3-bis(3,5-dimethylphenylcarbamate) showed the highest enantioselectivity towards the tested chiral analytes; the phenyl-heterogeneous (Ph-hetero) CS of amylose 6-(4-methylbenzamido)-6-deoxy-2,3-bis(3,5-dimethylphenylcarbamate) baseline separated the most chiral analytes; the phenyl-homogeneous (Ph-homo) CS of amylose 6-(3,5-dimethylbenzamido)-6-deoxy-2,3-bis(3,5-dimethylphenylcarbamate) also exhibited a good enantioseparation capability among the developed CSs. Regarding Ph-hetero CSs, the enantioselectivity depended on the combination of the substituent at 6-position and that at 2- and 3-positions; as for Ph-homo CSs, the enantioselectivity was related to the substituent at 2-, 3-, and 6-positions; with respect to 6-nonPh CSs, the retention factor of most analytes on the corresponding CSPs was lower than that on Ph-hetero and Ph-homo CSPs in the same mobile phases, indicating π-π interactions did occur during enantioseparation. Although the substituent at 6-position could not provide π-π interactions, the 6-nonPh CSs demonstrated an equivalent or even higher enantioselectivity compared with the Ph-homo and Ph-hetero CSs.

13.
CNS Neurosci Ther ; 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34605602

RESUMO

AIMS: Secondary gliosarcoma (SGS) rarely arises post treatment of primary glioblastoma multiforme (GBM), and contains gliomatous and sarcomatous components. The origin and clonal evolution of SGS sarcomatous components remain uncharacterized. Therapeutic radiation is mutagenic and can induce sarcomas in patients with other tumor phenotypes, but possible causal relationships between radiotherapy and induction of SGS sarcomatous components remain unexplored. Herein, we investigated the clonal origin of SGS in a patient with primary GBM progressing into SGS post-radiochemotherapy. METHODS: Somatic mutation profile in GBM and SGS was examined using whole-genome sequencing and deep-whole-exome sequencing. Mutation signatures were characterized to investigate relationships between radiochemotherapy and SGS pathogenesis. RESULTS: A mutation cluster containing two founding mutations in tumor-suppressor genes NF1 (variant allele frequency [VAF]: 50.0% in GBM and 51.1% in SGS) and TP53 (VAF: 26.7% in GBM and 50.8% in SGS) was shared in GBM and SGS. SGS exhibited an overpresented C>A (G>T) transversion (oxidative DNA damage signature) but no signature 11 mutations (alkylating-agents - exposure signature). Since radiation induces DNA lesions by generating reactive oxygen species, the mutations observed in this case of SGS were likely the result of radiotherapy rather than chemotherapy. CONCLUSIONS: Secondary gliosarcoma components likely have a monoclonal origin, and the clone possessing mutations in NF1 and TP53 was likely the founding clone in this case of SGS.

14.
Biomed Pharmacother ; 144: 112001, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34624677

RESUMO

Melatonin, mostly released by the pineal gland, is a circadian rhythm-regulated and multifunctional hormone. Great advances in melatonin research have been made, including its role in rhythms of the sleep-wake cycle, retardation of ageing processes, as well as antioxidant or anti-inflammatory functions. Melatonin can scavenge free radicals such as reactive oxygen species (ROS), a key factor in reproductive functions. Melatonin plays an important role in oocyte maturation, fertilization and embryonic development as well. The concurrent use of melatonin increases the number of mature oocytes, the fertilization rate, and number of high-quality embryos, which improves the clinical outcome of assisted reproductive technology (ART). This review discusses the relationship between melatonin and human reproductive function, and potential clinical applications of melatonin in the field of reproductive medicine.

15.
Chem Sci ; 12(36): 11936-11954, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34667561

RESUMO

With the rapid development of the Internet of Things (IoTs), photovoltaics (PVs) has a vast market supply gap of billion dollars. Moreover, it also puts forward new requirements for the development of indoor photovoltaic devices (IPVs). In recent years, PVs represented by organic photovoltaic cells (OPVs), silicon solar cells, dye-sensitized solar cells (DSSCs), etc. considered for use in IoTs mechanisms have also been extensively investigated. However, there are few reports on the indoor applications of perovskite devices, even though it has the advantages of better performance. In fact, perovskite has the advantages of better bandgap adjustability, lower cost, and easier preparation of large-area on flexible substrates, compared with other types of IPVs. This review starts from the development status of IoTs and investigates the cost, technology, and future trends of IPVs. We believe that perovskite photovoltaics is more suitable for indoor applications and review some strategies for fabricating high-performance perovskite indoor photovoltaic devices (IPVs). Finally, we also put forward a perspective for the long-term development of perovskite IPVs.

16.
Artigo em Inglês | MEDLINE | ID: mdl-34668408

RESUMO

BACKGROUND: Identifying protein biomarkers for chronic obstructive pulmonary disease (COPD) has been challenging. Most previous studies have utilized individual proteins or pre-selected protein panels measured in blood samples. Mass spectrometry proteomic studies of lung tissue have been based on small sample sizes. STUDY DESIGN AND METHODS: We utilized mass spectrometry proteomic approaches to discover protein biomarkers from 150 lung tissue samples representing COPD cases and controls. Top COPD-associated proteins were identified based on multiple linear regression analysis with false discovery rate (FDR) < 0.05. Correlations between pairs of COPD-associated proteins were examined. Machine learning models were also evaluated to identify potential combinations of protein biomarkers related to COPD. RESULTS: We identified 4407 proteins passing quality controls. Twenty-five proteins were significantly associated with COPD at FDR < 0.05, including Interleukin 33, Ferritin (light chain and heavy chain), and two proteins related to caveolae (CAV1 and CAVIN1). Multiple previously reported plasma protein biomarkers for COPD were not significantly associated with proteomic analysis of COPD in lung tissue, although RAGE was borderline significant. Eleven pairs of top significant proteins were highly correlated (r > 0.8), including several strongly correlated with RAGE (EHD2 and CAVIN1). Machine learning models using Random Forests with the top 5% of protein biomarkers demonstrated reasonable accuracy (0.766) and AUC (0.702) for COPD prediction. INTERPRETATION: Mass spectrometry proteomic analysis of lung tissue is a promising approach for the identification of biomarkers for COPD.

17.
Life (Basel) ; 11(9)2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34575089

RESUMO

Non-small cell lung cancer is a major lethal subtype of epithelial lung cancer, with high morbidity and mortality. The single-cell sequencing technique plays a key role in exploring the pathogenesis of non-small cell lung cancer. We proposed a computational method for distinguishing cell subtypes from the different pathological regions of non-small cell lung cancer on the basis of transcriptomic profiles, including a group of qualitative classification criteria (biomarkers) and various rules. The random forest classifier reached a Matthew's correlation coefficient (MCC) of 0.922 by using 720 features, and the decision tree reached an MCC of 0.786 by using 1880 features. The obtained biomarkers and rules were analyzed in the end of this study.

18.
ACS Appl Mater Interfaces ; 13(38): 45924-45934, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34520164

RESUMO

Skin-like electronics that can provide comprehensively tactile sensing is required for applications such as soft robotics, health monitoring, medical treatment, and human-machine interfaces. In particular, the capacity to monitor the contact parameters such as the magnitude, direction, and contact location of external forces is crucial for skin-like tactile sensing devices. Herein, a flexible electronic skin which can measure and discriminate the contact parameters in real time is designed. It is fabricated by integrating the three-dimensional (3D) hollow MXene spheres/Ag NW hybrid nanocomposite-based embedded stretchable electrodes and T-ZnOw/PDMS film-based capacitive pressure sensors. To the best of our knowledge, it is the first stretchable electrode to utilize the 3D hollow MXene spheres with the essential characteristic, which can effectively avoid the drawbacks of stress concentration and shedding of the conductive layer. The strain-resistance module and the pressure-capacitance module show the excellent sensing performance in stability and response time, respectively. Moreover, a 6 × 6 sensor array is used as a demonstration to prove that it can realize the multiplex detection of random external force stimuli without mutual interference, illustrating its potential applications in biomimetic soft wearable devices, object recognition, and robotic manipulation.

19.
Andrology ; 2021 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-34536053

RESUMO

BACKGROUND: Currently, there is no consensus on the effect of obstructive sleep apnea on male serum testosterone levels. This systematic review and meta-analysis aimed to determine the association between obstructive sleep apnea and male serum testosterone level. METHODS: The literature related to obstructive sleep apnea and male serum testosterone in the PubMed, Embase, and Cochrane Library databases were searched from their inception to June 10, 2021. Data were pooled using the Stata 15 software. We performed a subgroup analysis of studies after matching the age and body mass index, as well as according to the severity of obstructive sleep apnea. RESULTS: Eighteen studies involving 1823 men were included in the systematic review and meta-analysis. A significant inverse association between obstructive sleep apnea and male serum testosterone (SMD = -0.76; 95% CI: -1.18, -0.33; p = 0.001) was found. After adjusting for age and body mass index, this inverse association still existed (SMD = -0.8; 95% CI = -1.41, -0.18, p = 0.012). According to the subgroup analysis of obstructive sleep apnea severity, our results showed that serum testosterone was not significantly decreased in mild (SMD = -0.58; 95% CI = -1.88, 0.73, p = 0.386) and moderate obstructive sleep apnea patients (SMD = -0.94; 95% CI = -2.04, 0.15, p = 0.092), whereas it was significantly reduced in patients with severe obstructive sleep apnea (SMD = -1.21; 95% CI = -2.02, -0.41, p = 0.003). CONCLUSIONS: Obstructive sleep apnea is inversely associated with male serum testosterone levels, independent of body mass index and age. Notably, the severity of obstructive sleep apnea is also correlated with male serum testosterone, which is significantly reduced in patients with severe obstructive sleep apnea.

20.
Urology ; 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34563544

RESUMO

OBJECTIVE: To evaluate the effect of the funnel-shaped structure of the bladder neck in patients with benign prostatic hyperplasia (BPH), we investigated the correlation of the bladder neck angle (BNA) with the urinary flow rate and lower urinary tract symptoms (LUTS). METHODS: The cohort comprised 281 first-visit patients. The anatomical prostatic parameters, including BNA, total prostate volume, prostate urethral angle, intravesical prostatic protrusion, and prostate urethral length, were measured by transrectal ultrasonography. The International Prostate Symptom Score (IPSS), postvoid residual urine volume, and uroflowmetric measurements were evaluated. RESULTS: As revealed by multivariable linear regression analysis, BNA was independently associated with the peak flow rate (Qmax) and IPSS. The mean BNA significantly differed according to the severity of prostate symptoms and Qmax, and the mean BNA was 85.5°±17.4° and 89.84°±16.31° in patients with severe prostate symptoms and a Qmax<10 ml/s (P<0.001 and <0.001, respectively). The linear regression analysis showed that BNA was positively associated with the total IPSS (R=0.718, P<0.001) and inversely associated with Qmax (R=0.569, P<0.001). In addition, the mean BNA in patients with acute urine residual (AUR) was higher than that in patients without AUR (86.04°±16.36° VS 63.75°±15.14°, P<0.05). CONCLUSIONS: BNA is significantly correlated with LUTS and urinary flow rate in patients with BPH. Our findings suggest that BNA could play an important role in the pathogenic mechanism of BPH and AUR.

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