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1.
Front Physiol ; 13: 834077, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35492613

RESUMO

Rutin is a flavanol-type polyphenol that consists of flavanol quercetin and the disaccharide rutinose, which has been reported to exert various biological effects such as antioxidant and anti-inflammatory activities. It is not clear whether rutin has a protective effect on sepsis-induced cardiomyopathy (SIC). In this study, we used male C57BL/6 mice and cecal ligation and puncture (CLP) surgery to establish the model of SIC. Rutin was precautionarily treated (50, 100, 200 mg/kg per day, 7 days) before CLP. The results showed that rutin pretreatment (100, 200 mg/kg per day, 7 days) reduced the mortality of murine sepsis. We chose the 100 mg/kg dose for further studies. Mice were pretreatment with rutin (100 mg/kg per day, 7 days) before subjected to CLP, and myocardial tissue and blood samples were collected 24 h after CLP. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and cTNT decreased, while interleukin-10 (IL-10) increased with rutin pretreatment. The cardiomyocytes apoptosis and mitochondrial dysfunction were also alleviated with rutin pretreatment. In conclusion, this study confirmed the efficacy of rutin-enriched diet in the prophylaxis of cardiac apoptosis and cardiac injury induced by CLP in mouse model. It provides a potential new approach on SIC prophylaxis in sepsis.

2.
Artigo em Inglês | MEDLINE | ID: mdl-35512265

RESUMO

BACKGROUND: Motor resilience proteins have not been identified. This proteome-wide discovery study sought to identify proteins that may provide motor resilience. METHODS: We studied the brains of older decedents with annual motor testing, postmortem brain pathologies and proteome-wide data. Parkinsonism was assessed using 26 items of a modified United Parkinson Disease Rating Scale. We used linear mixed-effect models to isolate motor resilience, defined as the person-specific estimate of progressive parkinsonism after controlling for age, sex and ten brain pathologies. A total of 8356 high abundance proteins were quantified from dorsal lateral prefrontal cortex using tandem mass tag and liquid chromatography mass spectrometry. RESULTS: There were 391 older adults (70% female), mean age 80 years at baseline and 89 years at death. Five proteins were associated with motor resilience: A higher level of AP1B1 (Est., -0.504, S.E.,0.121, p=3.12 x10 -5) and GNG3 (Est., -0.276, S.E., 0.068, p=4.82 x10 -5) were associated with slower progressive parkinsonism. By contrast, a higher level of TTC38 (Est.,0.140, S.E.0.029, p=1.87x10 -6), CARKD (Est.,0.413, S.E.,0.100, p=3.50 x10 -5) and ABHD14B (Est.,0.175, S.E., 0.044, p=6.48 x10 -5) were associated with faster progressive parkinsonism. Together, these five proteins accounted for almost 25% of the variance of progressive parkinsonism above the 17% accounted for by ten indices of brain pathologies. DISCUSSION: Cortical proteins may provide more or less motor resilience in older adults. These proteins are high value therapeutic targets for drug discovery that may lead to interventions that maintain motor function despite the accumulation of as yet untreatable brain pathologies.

3.
Plant Biol (Stuttg) ; 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35491433

RESUMO

Homeodomain-leucine zipper (HD-Zip) proteins are plant-specific transcription factors that play important roles in different biological processes, especially leaf development. However, no studies to date have identified the HD-Zip genes in Liriodendron chinense nor characterized their functions. We identified the HD-Zip genes in L. chinense by analyzing the phylogeny, chromosomal location, structure, conserved motif, cis-regulatory elements, synteny, post-transcriptional regulation, and expression patterns of these genes during leaf development. A total of 36 LcHD-Zip genes were identified and divided into four subfamilies (HD-Zip Ⅰ to Ⅳ). Synteny analysis revealed that segmental duplication was the main force driving the expansion of LcHD-Zip genes. These 36 LcHD-Zip genes exhibited 11 different expression patterns. Pattern 1, 2, 3, 4, 6, 7, 8, and 9 genes may play important roles in leaf development, such as leaf initiation, leaf polarity establishment, leaf shape development, phytohormone-mediated leaf growth, and leaf epidermal structure formation. Four HD-Zip Ⅲ genes were targeted by microRNAs (miRNAs), and the miR165/166a-HD-Zip regulatory module formed regulates leaf initiation and leaf polarity establishment. Overall, LcHD-Zip genes might play key roles in the leaf development of L. chinense. This work provides a foundation for the functional verification of the HD-Zip genes identified in this study.

4.
Front Psychol ; 13: 845355, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35572285

RESUMO

Objective: To examine the relation between mobile phone addiction and high school students' depression, and its inner mechanism-the sequential mediating roles of the cyberbullying victimization and the cyberbullying perpetration in this relationship. Methods: 1297 high school students were recruited to complete the Smartphone Addiction Scale, European Cyberbullying Intervention Project Questionnaire and the Center for Epidemiological Studies Depression Scale. Results: (1) Mobile phone addiction was positively correlated with and high school students' depression; (2) cyberbullying victimization and the cyberbullying perpetration significantly mediated the relation between mobile phone addiction and high school students' depression, which contained tow mediating paths-the independent mediating effects of cyberbullying victimization and the sequential mediating effect of cyberbullying victimization and the cyberbullying perpetration; (3) there are gender differences in the sequential mediation model, and boys who are victims of cyberbullying are more likely to develop into cyberbullying perpetrators than girls. Conclusion: The results of this study indicate that depression among high school students with mobile phone addiction can be eliminated through the development of cyberbullying victimization and the cyberbullying perpetration.

5.
ACS Omega ; 7(17): 14920-14932, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35557686

RESUMO

An early and sustained immune response can lead to chronic inflammation after the implant is placed in the body. The implantable materials with immunomodulatory effects can reduce the body's immune response and promote the formation of ideal osseointegration between the implants and bone tissue. In this study, zinc-coated titanium micro-arc oxide coating was prepared on titanium surface by micro-arc oxidation. The physical properties, anti-inflammation, and osteogenesis of the material were evaluated. We have physically characterized the surface structure of the coatings by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and atomic force microscopy (AFM) and detected the release of Zn2+ from the coating surface by inductively coupled optical plasma emission spectrometry (ICP-OES). The BMSCs were inoculated on the surface of the coating, and the biocompatibility of the coating was evaluated by CCK-8 analysis and living and dead cell staining. The osteogenic effect of the layer on BMSCs was evaluated by alkaline phosphatase (ALP) assays, osteocalcin (OCN) immunofluorescence, and quantitative polymerase chain reaction (q-PCR). The survival status of RAW264.7 on the coating surface and the mRNA expression of the associated proinflammatory markers, tumor necrosis factor-α (TNF-α), cluster of differentiation 86 (CD86), and inducible nitric oxide (INOS) were detected by CCK-8 analysis and q-PCR. In parallel, the cell counting kit-8 (CCK-8) analysis and q-PCR screened and evaluated the effective concentration of Zn2+ anti-inflammatory in vitro. The results show that the coating has good physical characterization, and Zn is uniformly bound to the surface of titanium and shows stable release and good biocompatibility to BMSCs, downregulating the expression of inflammation-related genes promoting the bone formation of BMSCs. We have successfully prepared zinc-coated micro-arc titanium oxide coating on the titanium surface, which has good osteogenesis and great anti-inflammatory potential and provides a new way for osseointegration in the implant.

6.
Nanomaterials (Basel) ; 12(9)2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35564194

RESUMO

Selective hydrogenation of 1,3-butadiene (BD) is regarded as the most promising route for removing BD from butene streams. Bimetallic Pd-Ni catalysts with changed Pd/Ni molar ratios and monometallic Pd catalysts were synthesized using two differently structured metal-organic framework supports: UiO-66 and UiO-66-NH2. The effects of the structure of support and the molar ratio of Pd/Ni on the catalytic property of selective BD hydrogenation were studied. The Pd-Ni bimetallic supported catalysts, PdNi/UiO-66 (1:1) and PdNi/UiO-66-NH2 (1:1), exhibited fine catalytic property at low temperature. Compared with UiO-66, UiO-66-NH2 with a certain number of alkaline sites could reduce the catalytic activity for the BD hydrogenation reaction. However, the alkaline environment of UiO-66-NH2 is helpful to improve the butene selectivity. PdNi/UiO-66-NH2 (1:1) catalyst presented better stability than PdNi/UiO-66 (1:1) under the reaction conditions, caused by the strong interaction between the -NH2 groups of UiO-66-NH2 and PdNi NPs. Moreover, the PdNi/UiO-66-NH2 (1:1) catalyst presented good reproducibility in the hydrogenation of BD. These findings afford a beneficial guidance for the design and preparation of efficient catalysts for selective BD hydrogenation.

7.
J Colloid Interface Sci ; 622: 117-125, 2022 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-35490615

RESUMO

Silver nanoparticles (AgNPs) have been widely recognised as effective antibacterial materials in textiles for enhancing wound healing. However, high loadings of AgNPs are toxic and expensive. Thus, it is ideal to prepare AgNPs in a favourable nanostructure for stable and effective conjugation with the textile carrier by selecting a reductant and stabiliser that contributes to the antibacterial effect. Here, silver nanoparticles/vanadium oxide nanowires (Ag/VOx NWs) were prepared via a one-step reduction strategy using vanadium oxide quantum dots (VOx QDs) as both the reductant and stabiliser. VOx QDs possess antibacterial properties, which aid in minimising the applied silver content while enhancing bactericidal performance. Silver can self-aggregate into nanoparticles as well as promote the formation of vanadium oxide nanowires (VOx NWs). Accordingly, the Ag/VOx NWs exhibited remarkable antibacterial effects against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The nanowire structure of the Ag/VOx NWs was favourable for effective loading into a sodium alginate (SA) gel fabric to form a wound dressing. The effective loading of Ag/VOx NWs on SA was conducive to the complete dispersion of the bacteriostatic agent and enhanced the antibacterial activity of AgNPs. The wound dressing efficiently suppressed the growth of wound bacteria and promoted wound healing in vivo.

8.
Psychiatry Res ; 313: 114592, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35526422

RESUMO

This meta-analysis examined the effects of noninvasive brain stimulation (NIBS) for treating eating disorders (EDs), evaluating the difference between repetitive transcranial magnetic stimulation (rTMS) and transcranial direct current stimulation (tDCS). We identified 15 studies on EDs, including 9 RCTs (266 participants) and 6 one-arm trials (59 participants). The pooled effect sizes indicated that NIBS plays a positive role in improving the eating symptoms of ED patients. Although its long-term effects need to be further explored, this treatment has the potential to be an important supplement to conventional ED therapy.

10.
Front Neurosci ; 16: 854219, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35527818

RESUMO

Background: Rehabilitation of post-stroke dysphagia is an urgent clinical problem, and repetitive transcranial magnetic stimulation (rTMS) has been widely used in the study of post-stroke function. However, there is no reliable evidence-based medicine to support the effect of rTMS on post-stroke dysphagia. This review aims to evaluate the effectiveness and safety of rTMS on post-stroke dysphagia. Methods: English-language literature published before December 20, 2021, were searched in six electronic databases. Identified articles were screened, data were extracted, and the methodological quality of included trials was assessed. Meta-analysis was performed using RevMan 5.3 software. The GRADE method was used to assess the quality of the evidence. Results: A total of 10 studies with 246 patients were included. Meta-analysis showed that rTMS significantly improved overall swallowing function (standardized mean difference [SMD]-0.76, 95% confidence interval (CI)-1.07 to-0.46, p < 0.0001, n = 206; moderate-quality evidence), Penetration Aspiration Scale (PAS) (mean difference [MD]-1.03, 95% CI-1.51 to-0.55, p < 0.0001, n = 161; low-quality evidence) and Barthel index scale (BI) (MD 23.86, 95% CI 12.73 to 34.99, p < 0.0001, n = 136; moderate-quality evidence). Subgroup analyses revealed that (1) rTMS targeting the affected hemisphere and targeting both hemispheres significantly enhanced overall swallowing function and reduced aspiration. (2) Low-frequency rTMS significantly enhanced overall swallowing function and reduced aspiration, and there was no significant difference between high-frequency rTMS and control group in reducing aspiration (p = 0.09). (3) There was no statistical difference in the dropout rate (low-quality evidence) and adverse effects (moderate-quality evidence) between the rTMS group and the control group. Conclusion: rTMS improved overall swallowing function and activity of daily living ability and reduced aspiration in post-stroke patients with good acceptability and mild adverse effects.

11.
J Appl Gerontol ; : 7334648221095514, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35543174

RESUMO

Objectives: Little is known about the contribution of positive psychological factors, such as purpose in life, to healthcare and financial decision making in aging. Here, we examined the relationship between purpose and decision making and tested the hypothesis that purpose benefits decision making, particularly when cognition is limited. Methods: Participants were 1081 community-based older adults without dementia. Healthcare and financial decision making was measured via a 12-item performance-based instrument. Purpose was measured via a 10-item scale. Results: In a linear regression model adjusted for age, sex, and education, higher global cognition was associated with better performance on the decision making measure, as expected. Purpose was not directly related to decision making. However, the interaction of purpose with cognition was significant, such that greater purpose was associated with better decision making among persons with lower cognition. Discussion: Purpose in life may promote better decision making among older adults with lower cognition.

12.
Environ Sci Technol ; 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35546088

RESUMO

Efficient degradation of organic pollutants by oxidative radicals is challenging in the complex soil environment because of the invalid consumption of radicals by nontarget background substances and the generation of secondary halogenated organic pollutants. Nonradical-based oxidation is a promising pollutant removal method due to its high selectivity and environmental adaptability. Herein, a biochar-supported sheetlike CuO (e-CuO@BC) was developed, which exhibited efficient activation of peroxydisulfate (PDS) via nonradical pathways. The activation mechanisms were identified as (i) formation of surface-bonding active complexes via an outer-sphere interaction between e-CuO@BC and PDS and (ii) the continuous generation of 1O2 by the cycling of the Cu(I)/Cu(II) redox couple. In addition, the activation of PDS primarily occurred at the crystal facet (001) of e-CuO occupied by Cu atoms and was well facilitated by the Cu-O-C bond, which induced electron-rich centers around CuO. Two oxidative species from PDS activation, including surface-bonding active complexes and 1O2, showed a highly selective degradation toward electron-rich pollutants. Moreover, a highly efficient mineralization of organic pollutants and an effective inhibition on the generation of toxic byproducts (i.e., halogenated organics) was indicated by the intermediate and final degradation products. This study provides a comprehensive understanding of the heterogeneous activation process of PS by the e-CuO@BC catalyst for electron-rich organic pollutant removal.

13.
J Am Chem Soc ; 2022 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-35578912

RESUMO

Programmable DNA nanotechnology has generated some of the most intricate self-assembled nanostructures and has been employed in a growing number of applications, including functional nanomaterials, nanofabrication, biophysics, photonics, molecular machines, and drug delivery. An important design rule for DNA nanostructures is to minimize the mechanical stress to reduce the potential energy in these nanostructures whenever it is possible. This work revisits the DNA gridiron design consisting of Holliday junctions and compares the self-assembly of the canonical DNA gridiron with a new design of DNA gridiron, which has a higher degree of mechanical stress because of the interweaving of DNA helices. While the interweaving DNA gridiron indeed exhibits lower yield, compared to its canonical counterpart of a similar size, we discover that the mechanical stress within the interweaving gridiron can promote the formation of the two-dimensional crystalline lattice instead of nanotubes. Furthermore, tuning the design of interweaving gridiron leads to the change of overall crystal size and regularity of geometry. Interweaving DNA double helices represents a new design strategy in the self-assembly of DNA nanostructures. Furthermore, the discovery of the new role of mechanical stress in the self-assembly of DNA nanostructures provides useful knowledge to DNA nanotechnology practitioners: a more balanced view regarding mechanical stress can be considered when designing future DNA nanostructures.

14.
ACS Appl Mater Interfaces ; 14(15): 17195-17207, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35384659

RESUMO

The efficient hydrogenation of biomass-derived levulinic acid (LA) to value-added γ-valerolactone (GVL) based on nonprecious metal catalysts under mild conditions is crucial challenge because of the intrinsic inactivity and instability of these catalysts. Herein, a series of highly active and stable carbon-encapsulated Co/ZnO@C-X (where X = 0.1, 0.3, 0.5, the molar ratios of Zn/(Co+Zn)) heterojunction catalysts were obtained by in situ pyrolysis of bimetal CoZn MOF-74. The optimal Co/ZnO@C-0.3 catalyst could achieve 100% conversion of LA and 98.35% selectivity to GVL under mild conditions (100 °C, 5 bar, 3 h), which outperformed most of the state-of-the-art catalysts reported so far. Detailed characterizations, experimental investigations, and theoretical calculations revealed that the interfacial interaction between Co and ZnO nanoparticles (NPs) could promote the dispersibility and air stability of the active Co0 for the activation of H2. Moreover, the strong Co-ZnO interaction also enhanced the Lewis acidity of the Co/ZnO interface, contributing to the adsorption of LA and the esterification of intermediates. The synergy between the hydrogenation sites and the Lewis acid sites at the Co/ZnO interface enabled the conversion of LA to GVL with high efficiency. In addition, benefiting from the Co-ZnO interfacial interaction as well as the unique carbon-encapsulated structure of the heterojunction catalyst, the recyclability was also greatly improved and the yield of GVL was nearly unchanged even after six cycles.

15.
Int J Biol Sci ; 18(6): 2249-2260, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35414782

RESUMO

Hepatocellular carcinoma (HCC) is a major cause of cancer-related death worldwide. Although it has been known that hepatic stellate cells (HSCs) play critical roles in the development and progression of HCC, the molecular mechanism underlying crosstalk between HSCs and cancer cells still remains unclear. Here, we investigated the interactions between HSCs and cancer cells through an indirect co-culture system. The expressions of cellular and exosomal miR-148a-3p were evaluated by quantitative real-time PCR. Cell counting kit-8 was used for evaluating cell growth in vitro. Cell migration and invasion ability were evaluated by wound-healing and Transwell assays. Western blot, quantitative real-time PCR and Luciferase reporter assay were performed to determine the target gene of miR-148a-3p. A xenograft liver cancer model was established to study the function of exosomal miR-148a-3p in vivo. We found that miR-148a-3p was downregulated in co-cultured HSCs and overexpression of miR-148a-3p in HSCs impaired the proliferation and invasiveness of HCC both in vitro and in vivo. Moreover, further study showed that the miR-148a-3p was also downexpressed in HSCs-derived exosomes, and increased HSCs-derived exosomal miR-148a-3p suppressed HCC tumorigenesis through ITGA5/PI3K/Akt pathway. In conclusion, our study demonstrated that exosome-depleted miR-148a-3p derived from activated HSCs accelerates HCC progression through ITGA5/PI3K/Akt axis.


Assuntos
Carcinoma Hepatocelular , Exossomos , Neoplasias Hepáticas , MicroRNAs , Carcinoma Hepatocelular/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/genética , Exossomos/genética , Exossomos/metabolismo , Regulação Neoplásica da Expressão Gênica , Células Estreladas do Fígado/metabolismo , Humanos , Neoplasias Hepáticas/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo
16.
Artigo em Inglês | MEDLINE | ID: mdl-35445870

RESUMO

PURPOSE: Chimeric antigen receptor (CAR) T cell therapy has demonstrated clinical success in treating haematologic malignancies but has not been effective against solid tumours thus far. Trop2 is a tumour-related antigen broadly overexpressed on a variety of tumours and has been reported as a promising target for pancreatic cancers. Our study aimed to determine whether CAR T cells designed with a fully human Trop2-specific single-chain fragment variable (scFv) can be used in the treatment of Trop2-positive pancreatic tumours. METHODS: We designed Trop2-targeted chimeric antigen receptor engineered T cells with a novel human anti-Trop2 scFv (2F11) and then investigated the cytotoxicity, degranulation, and cytokine secretion profiles of the anti-Trop2 CAR T cells when they were exposed to Trop2 + cancer cells in vitro. We also studied the antitumour efficacy and toxicity of Trop2-specific CAR T cells in vivo using a BxPC-3 pancreatic xenograft model. RESULTS: Trop2-targeted CAR T cells designed with 2F11 effectively killed Trop2-positive pancreatic cancer cells and produced high levels of cytotoxic cytokines in vitro. In addition, Trop2-targeted CAR T cells, which persistently circulate in vivo and efficiently infiltrate into tumour tissues, significantly blocked and even eliminated BxPC-3 pancreatic xenograft tumour growth without obvious deleterious effects observed after intravenous injection into NSG mice. Moreover, disease-free survival was efficiently prolonged. CONCLUSION: These results show that Trop2-targeted CAR T cells equipped with a fully human anti-Trop2 scFv could be a potential treatment strategy for pancreatic cancer and could be useful for clinical evaluation.

17.
Polymers (Basel) ; 14(8)2022 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-35458260

RESUMO

It is critical to develop efficient oxygen evolution reaction (OER) catalysts with high catalytic properties for overall water splitting. Electrocatalysts with enriched vacancies are crucial for enhancing the catalytic activity of OER through defect engineering. We demonstrated the dealloying method in a reducing alkaline solution using the Co5Al95 alloy foil as a precursor to produce a new oxygen-vacancy-rich cobalt hydroxide (OV-Co(OH)2) hierarchical dendrite. The as-synthesised OV-Co(OH)2 showed superior electrocatalytic activities toward OER when compared to pristine cobalt hydroxide (p-Co(OH)2), which had a low onset overpotential of only 242 mV and a small Tafel slope of 64.9 mV dec-1. Additionally, for the high surface area provided by the hierarchical dendrite, both p-Co(OH)2 and OV-Co(OH)2 showed a superior activity as compared to commercial catalysts. Furthermore, they retained good catalytic properties without remarkably decaying at an overpotential of 350 mV for 12 h. The as-made OV-Co(OH)2 has prospective applications as an anode electrocatalyst in electrochemical water-splitting technologies with the advantages of superior OER performances, large surface area and ease of preparation.

18.
Mol Pain ; 18: 17448069221097760, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35430901

RESUMO

Gout is a prevalent and painful inflammatory arthritis, and its global burden continues to rise. Intense pain induced by gout attacks is a major complication of gout. However, systematic studies of gout inflammation and pain are lacking. Using a monosodium urate (MSU) crystal-induced gout model, we performed genome-wide transcriptome analysis of the inflamed ankle joint, dorsal root ganglion (DRG), and spinal cord of gouty mice. Our results revealed important transcriptional changes, including highly elevated inflammation and broad activation of immune pathways in both the joint and the nervous system, in gouty mice. Integrated analysis showed that there was a remarkable overlap between our RNAseq and human genome-wide association study (GWAS) of gout; for example, the risk gene, stanniocalcin-1 (STC1) showed significant upregulation in all three tissues. Interestingly, when compared to the transcriptomes of human osteoarthritis (OA) and rheumatoid arthritis (RA) joint tissues, we identified significant upregulation of cAMP/cyclic nucleotide-mediated signaling shared between gouty mice and human OA with high knee pain, which may provide excellent drug targets to relieve gout pain. Furthermore, we investigated the common and distinct transcriptomic features of gouty, inflammatory pain, and neuropathic pain mouse models in their DRG and spinal cord tissues. Moreover, we discovered distinct sets of genes with significant differential alternative splicing or differential transcript usage in each tissue, which were largely not detected by conventional differential gene expression analysis approaches. Based on these results, our study provided a more accurate and comprehensive depiction of transcriptomic alterations related to gout inflammation and pain.


Assuntos
Gota , Ácido Úrico , Animais , Modelos Animais de Doenças , Estudo de Associação Genômica Ampla , Gota/induzido quimicamente , Gota/complicações , Gota/genética , Inflamação/complicações , Inflamação/genética , Camundongos , Dor/genética
19.
RSC Adv ; 12(16): 9653-9659, 2022 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-35424913

RESUMO

Products of main group elements from cross-coupling reactions have been shown to serve as Lewis acids, mediating further reactions of organic coupling products. Thus, the nickel-catalysed olefination of benzylic dithioacetal with MeMgI in benzene in a sealed Schlenk tube at 130 °C generates magnesium mercaptide which regioselectively converts 2-arylpropene into a dimer in good yield. Aryl iodide reacts with 2-propenylmagnesium bromide in the presence of 1,2-ethanedithiol and NiCl2(PPh3)2 to yield the same dimer. Replacement of the Grignard reagent by an organozinc reagent gives the dimers in a better yield.

20.
Artigo em Inglês | MEDLINE | ID: mdl-35391544

RESUMO

This study investigated the effects of different types of saline stress on the availability of cadmium (Cd) and bacterial growth. Changes in soil physicochemical properties and DTPA-Cd content as well as microbial responses after the addition of salts were measured. The addition of 18 g kg-1 of salts with NaCl and Na2SO4 increased the available Cd content by up to 17.80%-29.79%. Respiration rate, biomass, and relative bacterial growth decreased with increasing salt concentrations. Estimated salinity tolerance of bacterial communities based on pollution-induced community tolerance. The salinity tolerance index EC50 of the bacterial community was estimated by logistic equation and ranged from 4.32-12.63 g kg-1. Structural equation modeling showed that soil salinity stress significantly affected Cd availability and bacterial community, while bacterial growth characteristics also contributed to reducing available Cd. We conclude that saline stress can alter soil Cd availability in soils by affecting the growth characteristics of soil bacterial communities.

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