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1.
Nanoscale ; 15(4): 1609-1618, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36602001

RESUMO

Formaldehyde (HCHO) is widely viewed as a carcinogenic volatile organic compound in indoor air pollution that can seriously threaten human health and life. Thus, there is a critical need to develop gas sensors with improved sensing performance, including outstanding selectivity, low operating temperature, high responsiveness, and short recovery time, for HCHO detection. Currently, doping is considered an effective strategy to raise the sensing performance of gas sensors. Herein, various rare earth elements-doped indium oxide (RE-In2O3) nanospheres were fabricated as gas sensors for improved HCHO detection via a facile and environmentally solvothermal method. Such RE-In2O3 nanosphere-based sensors exhibited remarkable gas-sensing performance, including a high selectivity and stability in air. Compared with pure, Yb-, Dy-doped In2O3 and different La ratios doped into In2O3, 6% La-doped In2O3 (La-In2O3) nanosphere-based sensors demonstrated a high response value of 210 to 100 ppm at 170 °C, which was around 16 times higher than that of the pure In2O3 sensor, and also exhibited a detection limit of 10.9 ppb, and a response time of 30 s to 100 ppm HCHO with a recovery time of 160 s. Finally, such superior sensing performance of the 6% La-In2O3 sensors was proposed to be attributed to the synergistic effect of the large specific surface area and enhanced surface oxygen vacancies on the surface of In2O3 nanospheres, which produced chemisorbed oxygen species to release electrons and provided abundant reaction sites for HCHO gas. This study sheds new light on designing nanomaterials to build gas sensors for HCHO detection.

2.
World J Gastroenterol ; 29(1): 1-18, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36683709

RESUMO

Colorectal cancer (CRC) is one of the most common malignancies of the digestive tract, with the annual incidence and mortality increasing consistently. Oxaliplatin-based chemotherapy is a preferred therapeutic regimen for patients with advanced CRC. However, most patients will inevitably develop resistance to oxaliplatin. Many studies have reported that non-coding RNAs (ncRNAs), such as microRNAs, long non-coding RNAs, and circular RNAs, are extensively involved in cancer progression. Moreover, emerging evidence has revealed that ncRNAs mediate chemoresistance to oxaliplatin by transcriptional and post-transcriptional regulation, and by epigenetic modification. In this review, we summarize the mechanisms by which ncRNAs regulate the initiation and development of CRC chemoresistance to oxaliplatin. Furthermore, we investigate the clinical application of ncRNAs as promising biomarkers for liquid CRC biopsy. This review provides new insights into overcoming oxaliplatin resistance in CRC by targeting ncRNAs.


Assuntos
Neoplasias Colorretais , MicroRNAs , RNA Longo não Codificante , Humanos , Oxaliplatina/farmacologia , Oxaliplatina/uso terapêutico , RNA não Traduzido/genética , MicroRNAs/genética , MicroRNAs/uso terapêutico , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia
3.
Spectrochim Acta A Mol Biomol Spectrosc ; 291: 122384, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36689908

RESUMO

Development of accurate and efficient TCs residue analysis methods is of great significance for the protection of environment, food safety and public health. Herein, a dual-responsive ratiometric fluorescence sensor being capable of simple and sensitive detection of tetracycline (TC) was presented, which was constructed by immobilizing europium ions (Eu3+) onto the mercaptopropionic acid stabilized copper nanoclusters (MPA-Cu NCs). In the presence of TC, the red fluorescence of Eu3+ was enhanced through antenna effect (AE), while the green fluorescence of MPA-Cu NCs was quenched through internal filter effect (IFE), leading to an obvious fluorescence color evolution from green to red for the probe solution. In addition to successful design of a smartphone-assisted colorimetric analysis platform for portable detection, a logic gate device capable of intelligently monitoring TC concentration is also designed.

4.
Med Biol Eng Comput ; 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36648692

RESUMO

Recent research on semi-supervised learning (SSL) is mainly based on the method of consistency regularization, which relies on domain-specific data augmentation. Pseudo-labeling is a more general method that has no such restrictions but performs limited by noisy training. We combine both approaches and focus on generating pseudo-labels using domain-independent weak augmentation. In this article, we propose ReFixMatch-LS and apply it to the classification of medical images. First, we reduce the impact of noisy artificial labels by label smoothing and consistent regularization. Then, by recording high-confidence pseudo-labels generated from each epoch during training, we reuse the generated pseudo-labels to train the model in the subsequent epochs. ReFixMatch-LS effectively increases the number of pseudo-labels and improves the model performance. We validate the effectiveness of ReFixMatch-LS on skin lesion diagnosis in the ISIC 2018 and ISIC 2019 challenge datasets, obtaining AUCs of 91.54%, 93.68%, 94.55%, and 95.47% on the four proportions of labeled data from ISIC 2018.

5.
Sci Total Environ ; 858(Pt 3): 160079, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36372182

RESUMO

Triclosan (TCS) and triclocarban (TCC) have become ubiquitous pollutants detected in human body with concentrations up to hundreds of nanomolar levels. Previous studies about the hepatic lipid accumulation induced by TCS and TCC were focused on pollutant itself, which showed weak or no effects. High-fat diet (HFD), as a known environmental factor contributing to lipid metabolism-related disorders, its synergistic action with environmental pollutants deserves concern. The present study aimed to demonstrate the combined effects and potential molecular mechanisms of TCS and TCC with HFD at cellular and animal levels. The in vitro studies showed that TCC and TCS alone had negligible impact on lipid accumulation in HepG2 cells but induced lipid deposition at nanomolar levels when co-exposure with fatty acid. TCC exhibited much higher induction effects than TCS, which was related to their differential regulatory roles in adipogenic-related genes expression. The in vivo studies showed that TCC had little influence on hepatic lipid accumulation in mice fed with normal diet (ND) but could exacerbate the lipid accumulation in mice fed with HFD. Meanwhile, TCC-induced dyslipidemia in mice fed with HFD was more significant than that fed with ND. Therefore, we speculated that TCC might increase the risk of nonalcoholic fatty liver disease (NAFLD) and atherosclerosis in HFD humans. Molecular mechanism studies showed that TCC and TCS could bind to and activate estrogen-related receptor α (ERRα) and ERRγ as well as regulate their expression. TCC had higher activity on ERRα and ERRγ than TCS, which explained partly the differential regulatory roles of two receptors in the lipid accumulation induced by TCC and TCS. This work revealed synergistic effects and molecular mechanisms of TCC and TCS with excessive fatty acid on the hepatic lipid metabolism, which provided a novel insight into the toxic mechanism of pollutants from the perspective of dietary habits.


Assuntos
Dieta Hiperlipídica , Triclosan , Humanos , Camundongos , Animais , Dieta Hiperlipídica/efeitos adversos , Triclosan/toxicidade , Ácidos Graxos , Estrogênios , Lipídeos
6.
Org Lett ; 25(1): 200-204, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36546845

RESUMO

A Lewis acid mediated conjugate addition of isocyanides to ß-hydroxy-α-diazo carbonyls has been developed for the first time. The reaction realizes the efficient construction of quaternary carbon centers and provides a novel and efficient strategy for the synthesis of ß-carboxamido-α-diazo carbonyls that would be otherwise difficult to form in a single step. Further applications, including synthesis of methylenecyclohexane, spiro-ß-lactam, and nitrogen-bridged tricyclic ß-lactam, demonstrated the tremendous potential of the coupling reaction.

7.
Talanta ; 255: 124205, 2023 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-36580812

RESUMO

Selective and sensitive determination of ceftriaxone sodium (CTR) trace residues is of great importance for food safety and environmental protection. Herein, a determination method based on ratiometric fluorescence and colorimetric method with nitrogen-rich carbon dots as fluorophore is reported. The functional surfaces of indole-derived carbon dots (I-CDs) containing nitrogen and carbon groups can be selectively bound to CTR by electrostatic forces, leading to a hindered conjugation system and deprotonation of the amine on the pyrrole ring, resulting in a distinct variety in fluorescence and absorption wavelength and intensity. With the addition of CTR, the fluorescence at 577 nm can be selectively quenched, accompanied by a new emission peak appeared at 507 nm. The limits of detection (LODs) were estimated to be 19.7 nM and 78.0 nM based on the ratiometric fluorescence method and colorimetric method, respectively. Finally, the in situ visual quantitative determination of CTR using this nanosensor was achieved by combining with the color recognizer of a smartphone, and the method was further validated by spike and recovery test in real water samples including milk, seawater, and tap water.


Assuntos
Ceftriaxona , Pontos Quânticos , Carbono/química , Nitrogênio , Pontos Quânticos/química , Corantes Fluorescentes/química , Água
8.
Anal Chem ; 2022 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-36463539

RESUMO

A fluorescent and photothermal dual-mode assay method was established for the detection of acetylcholinesterase (AChE) activity based on in situ formation of o-phenylenediamine (oPD) cascade polymers. First, copper metal-organic frameworks of benzenetricarboxylic acid (Cu-BTC) were screened out as nanozymes with excellent oxidase-like activity and confinement catalysis effect. Then, an ingenious oPD cascade polymerization strategy was proposed. That is, oPD was oxidized by Cu-BTC to oPD oligomers with strong yellow fluorescence, and oPD oligomers were further catalyzed to generate J-aggregation, which promotes the formation of oPD polymer nanoparticles with a high photothermal effect. By utilizing thiocholine (enzymolysis product of acetylthiocholine) to inhibit the Cu-BTC catalytic effect, AChE activity was detected through the fluorescence-photothermal dual-signal change of oPD oligomers and polymer nanoparticles. Both assay modes have low detection limitation (0.03 U L-1 for fluorescence and 0.05 U L-1 for photothermal) and can accurately detect the AChE activity of human serum (recovery 85.0-111.3%). The detection results of real serum samples by fluorescent and photothermal dual modes are consistent with each other (relative error ≤ 5.2%). It is worth emphasizing that this is the first time to report the high photothermal effect of oPD polymers and the fluorescence-photothermal dual-mode assay of enzyme activity.

9.
Small ; : e2206606, 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36461684

RESUMO

For complex cascade biocatalysis, multienzyme compartmentalization helps to optimize substrate transport channels and promote the orderly and tunable progress of step reactions. Herein, a simple and general synthesis strategy is proposed for the construction of a multienzyme biocatalyst by compartmentalizing glucose oxidase and horseradish peroxidase (GOx and HRP) within core-shell zeolite imidazole frameworks (ZIF)-8@ZIF-8 nanostructures. Owing to the combined effects of biomimetic mineralization and the fine regulation of the ZIF-8 growth process, the uniform shell encloses the seed (core) surface by epitaxial growth, and the bienzyme system is accurately localized in a controlled manner. The versatility of this strategy is also reflected in ZIF-67. Meanwhile, with the ability to covalently bind divalent metal ions, lithocholic acid (LCA) is used as a competitive ligand to improve the pore structure of the ZIF from a single micropore to a hierarchical micro/mesopore network, which greatly increases mass transfer efficiency. Furthermore, the multienzyme cascade reaction is exemplified by the oxidation of o-phenylenediamine (OPD). The findings show that the bienzyme assembly strategy significantly affects the biocatalytic efficiency mainly by influencing the utilization efficiency of the intermediate (Hydrogen peroxide, H2 O2 ) between the step reactions. This study sheds new light on facile synthetic routes to constructing in vitro multienzyme biocatalysts.

10.
Dalton Trans ; 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36464993

RESUMO

Since the abuse of antibiotic oxytetracycline (OTC) poses a serious threat to the environment and human health, it is of great importance to develop sensitive fluorescent probes for its rapid and in situ detection. Herein, a dual fluorescence response probe based on an aluminum-incorporated metal-organic framework (MOF) was presented for OTC assay. Unlike internal references that demonstrate an independent and stable fluorescence signal intensity in traditional dual-emissive probes, the fluorescence of rhodamine B immobilized in a prepared probe was gradually enhanced at a 585 nm emission wavelength with increasing concentrations of OTC under 405 nm excitation, while OTC also experienced an obvious fluorescence enhancement at a 521 nm emission wavelength due to a molecular conformation transition from the twisted to the extended state, realizing a molecular conformational transition-induced dual fluorescence enhancement for OTC detection under a single excitation wavelength. In addition to the mechanism exploration and double linear range for OTC quantification with nM level detection limits in solution, a paper-based portable test strip was successfully fabricated by loading the probe on glass fiber filter paper with an obvious fluorescence color change from orange to yellow upon increasing the addition of OTC. We expect that the proposed probe in this work would provide an example for the design of organic fluorophore-based sensors exhibiting multiple fluorescence responses under a single excitation.

11.
J Org Chem ; 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36469572

RESUMO

A photocatalyst-free radical cleavage of α-diazo sulfonium salts has been developed for the first time. The reaction provides an efficient method for the generation of diazomethyl radicals from α-diazosulfonium triflates under photochemical conditions. Utilizing the in situ generated diazomethyl radicals as key intermediate, the coupling cyclization reaction of α-diazosulfonium triflates with α-oxocarboxylic acids or alkynes has been achieved. The method affords a diverse set of important 2,5-disubstituted 1,3,4-oxadiazoles and 3,5-disubstituted-1H-pyrazoles with excellent regioselectivity in a single step. A reaction mechanism involving a radical pathway was further supported by control experiments and DFT calculations.

12.
J Agric Food Chem ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36454954

RESUMO

Curcumin, a polyphenol derived from turmeric, has multiple biological functions, such as anti-inflammatory, antioxidant, antibacterial and, above all, antitumor activity. Colorectal cancer is a common malignancy of the gastrointestinal tract with an extremely high mortality rate. However, the low bioavailability and poor targeting properties of curcumin generally limit its clinical application. In the present study, we designed a fusion protein GE11-HGFI as a nanodrug delivery system. The protein was connected by flexible linkers, inheriting the self-assembly properties of hydrophobin HGFI and the targeting ability of GE11. The data show that the encapsulation of curcumin by fusion protein GE11-HGFI can form uniform and stable nanoparticles with a size of only 80 nm. In addition, the nanocarrier had high encapsulation efficiency for curcumin and made it to release sustainably. Notably, the drug-loaded nanosystem selectively targeted colorectal cancer cells with high epidermal growth factor receptor expression, resulting in high aggregated concentrations of curcumin at tumor sites, thus showing a significant anticancer effect. These results suggest that the nanocarrier fusion protein has the potential to be a novel strategy for enhancing molecular bioactivity and drug targeting in cancer therapy.

13.
Front Med (Lausanne) ; 9: 999481, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36482909

RESUMO

Objectives: Early identification of traumatic brain injury (TBI) patients at a high risk of mortality is very important. This study aimed to compare the predictive accuracy of four scoring systems in TBI, including shock index (SI), modified shock index (MSI), age-adjusted shock index (ASI), and reverse shock index multiplied by the Glasgow Coma Scale (rSIG). Patients and methods: This is a retrospective analysis of a registry from the Taipei Tzu Chi trauma database. Totally, 1,791 patients with TBI were included. We investigated the accuracy of four major shock indices for TBI mortality. In the subgroup analysis, we also analyzed the effects of age, injury mechanism, underlying diseases, TBI severity, and injury severity. Results: The predictive accuracy of rSIG was significantly higher than those of SI, MSI, and ASI in all the patients [area under the receiver operating characteristic curve (AUROC), 0.710 vs. 0.495 vs. 0.527 vs. 0.598], especially in the moderate/severe TBI (AUROC, 0.625 vs. 0.450 vs. 0.476 vs. 0.529) and isolated head injury populations (AUROC 0.689 vs. 0.472 vs. 0.504 vs. 0.587). In the subgroup analysis, the prediction accuracy of mortality of rSIG was better in TBI with major trauma [Injury Severity Score (ISS) ≥ 16], motor vehicle collisions, fall injury, and healthy and cardiovascular disease population. rSIG also had a better prediction effect, as compared to SI, MSI, and ASI, both in the non-geriatric (age < 65 years) and geriatric (age ≥ 65 years). Conclusion: rSIG had a better prediction accuracy for mortality in the overall TBI population than SI, MSI, and ASI. Although rSIG have better accuracy than other indices (ROC values indicate poor to moderate accuracy), the further clinical studies are necessary to validate our results.

14.
Gastrointest Endosc ; 2022 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-36509113

RESUMO

BACKGROUND AND AIMS: For EUS-guided fine-needle biopsy (EUS-FNB) of solid pancreatic lesions (SPLs), the role of sampling strategy between a targeted biopsy and wide sampling has not been reported. This study aimed to investigate the benefits of the two sampling techniques on EUS-FNB with rapid on-site evaluation. METHODS: Patients with SPLs were prospectively enrolled and randomly assigned (1:1) to undergo EUS-FNB using either contrast guidance or the fanning technique. The primary outcome was the total number of passes required to establish a diagnosis, and the secondary outcomes were overall diagnostic accuracy and complication rates. RESULTS: A total of 118 patients were enrolled from February 2019 to January 2021, with 59 patients assigned to each group. There was no significant difference in the total number of passes required to establish a diagnosis between the contrast and fanning groups (median 1 [interquartile range 1-1] vs. 1 [1-2]; P = .629). The sensitivity, specificity, and diagnostic accuracy in the contrast and fanning groups were 100%, 66.7%, and 98.3%, versus 100%, 100%, and 100%, respectively (P = 1). An SPL size < 4 cm (odds ratio [OR], 2.47; 95% confidence interval [CI], 1.05-5.81; P = .037) and macroscopic visible core length > 1 cm (OR, 2.89; 95% CI, 1.07-7.84; P = .037) were independently associated with increased cytologic and histologic accuracy. CONCLUSION: The diagnostic accuracy of EUS-FNB with the fanning technique for SPLs was comparable with the contrast guidance technique. Without additional cost, EUS-FNB with the fanning technique may be preferred for SPLs.

15.
Front Bioeng Biotechnol ; 10: 1078137, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36518196

RESUMO

Iron as an essential element, is involved in various cellular functions and maintaining cell viability, cancer cell is more dependent on iron than normal cell due to its chief characteristic of hyper-proliferation. Despite that some of the iron chelators exhibited potent and broad antitumor activity, severe systemic toxicities have limited their clinical application. Polyaminoacids, as both drug-delivery platform and therapeutic agents, have attracted great interests owing to their different medical applications and biocompatibility. Herein, we have developed a novel iron nanochelator PL-DFX, which composed of deferasirox and hyperbranched polylysine. PL-DFX has higher cytotoxicity than DFX and this effect can be partially reversed by Fe2+ supplementation. PL-DFX also inhibited migration and invasion of cancer cells, interfere with iron metabolism, induce phase G1/S arrest and depolarize mitochondria membrane potential. Additionally, the anti-tumor potency of PL-DFX was also supported by organoids derived from clinical specimens. In this study, DFX-based iron nanochelator has provided a promising and prospective strategy for cancer therapy via iron metabolism disruption.

16.
Chem Sci ; 13(43): 12892-12898, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36519048

RESUMO

Tetracyclines are a class of antibiotics that exhibited potent activity against a wide range of Gram-positive and Gram-negative bacteria, yet only five members were isolated from actinobacteria, with two of them approved as clinical drugs. In this work, we developed a genome mining strategy using a TetR/MarR-transporter, a pair of common resistance enzymes in tetracycline biosynthesis, as probes to find the potential tetracycline gene clusters in the actinobacteria genome database. Further refinement using the phylogenetic analysis of chain length factors resulted in the discovery of 25 distinct tetracycline gene clusters, which finally resulted in the isolation and characterization of a novel tetracycline, hainancycline (1). Through genetic and biochemical studies, we elucidated the biosynthetic pathway of 1, which involves a complex glycosylation process. Our work discloses nature's huge capacity to generate diverse tetracyclines and expands the chemical diversity of tetracyclines.

17.
J Ethnopharmacol ; 304: 116020, 2022 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-36529254

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Sumu (Lignum sappan), the dry heartwood of Caesalpinia sappan L., is a traditional Chinese medicine used as an analgesic and anti-inflammatory agent. AIM OF THE STUDY: The study aspired to discover natural phosphodiesterase 4 (PDE4) inhibitors with dual anti-inflammatory and antioxidant activities from Sumu for the treatment of chronic obstructive pulmonary disease (COPD). MATERIALS AND METHODS: To accurately and efficiently identify natural PDE4 inhibitors from Sumu, molecular docking and molecular dynamics (MD) analysis methods were used for structure-based virtual screening of a self-built database of primary polyphenols in Sumu. According to the previous studies of Sumu and the free radical scavenging mechanism of polyphenols, the reported antioxidant components from Sumu and the potential antioxidants with the antioxidant pharmacophore of catechol and π-conjugated moieties were selected from the potential PDE4 inhibitors predicted by docking. Sappanone A, a potential PDE4 inhibitor with antioxidant activity from Sumu, was selected, calculated and synthesized to evaluate its dual anti-inflammatory and antioxidant functions in vitro and in vivo studies. Herein sappanone A was assayed for its inhibitory effects against PDE4 enzyme activity, tumor necrosis factor-alpha (TNF-α) production induced by lipopolysaccharide (LPS) in RAW264.7 macrophages and malondialdehyde (MDA) production induced by Fe2+ in mouse lung homogenate; sappanone A was also assayed for its abilities of radical (DPPH) scavenging, reducing Fe3+ and complexing Fe2+ in vitro. Additionally, LPS-induced acute lung injury (ALI) in mice was used to evaluate its anti-inflammatory activity as a PDE4 inhibitor in vivo, and the levels of TNF-α and total protein in bronchoalveolar lavage fluid (BALF) and myeloperoxidase (MPO) activity in the lung were assayed. RESULTS: The present study predicted and validated that sappanone A was a promising PDE4 inhibitor from Sumu with dual anti-inflammation and antioxidant activities from Sumu. In vitro, sappanone A remarkably inhibited PDE4 enzyme activity and reduced TNF-α production induced by LPS in RAW264.7 macrophages and MDA production induced by Fe2+ in mouse lung homogenate. Meanwhile, it showed outstanding abilities of scavenging DPPH radicals, reducing Fe3+ and complexing Fe2+. In vivo, sappanone A (25 mg/kg and 50 mg/kg, i.p., twice daily for 7 days) distinctly prevented LPS-induced ALI in mice by reducing the levels of TNF-α and total protein in BALF and MPO activity in the lung. CONCLUSION: Sappanone A is a natural PDE4 inhibitor with dual anti-inflammatory and antioxidant activities from the traditional Chinese medicine Sumu, which may be a promising therapeutic agent to prevent the vicious cycle of COPD inflammation and oxidative stress.

18.
Sensors (Basel) ; 22(24)2022 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-36560289

RESUMO

A variety of Chinese textual operational text data has been recorded during the operation and maintenance of the high-speed railway catenary system. Such defect text records can facilitate defect detection and defect severity analysis if mined efficiently and accurately. Therefore, in this context, this paper focuses on a specific problem in defect text mining, which is to efficiently extract defect-relevant information from catenary defect text records and automatically identify catenary defect severity. The specific task is transformed into a machine learning problem for defect text classification. First, we summarize the characteristics of catenary defect texts and construct a text dataset. Second, we use BERT to learn defect texts and generate word embedding vectors with contextual features, fed into the classification model. Third, we developed a deep text categorization network (DTCN) to distinguish the catenary defect level, considering the contextualized semantic features. Finally, the effectiveness of our proposed method (BERT-DTCN) is validated using a catenary defect textual dataset collected from 2016 to 2018 in the China Railway Administration in Chengdu, Lanzhou, and Hengshui. Moreover, BERT-DTCN outperforms several competitive methods in terms of accuracy, precision, recall, and F1-score value.


Assuntos
Semântica , Humanos , China , Mineração de Dados , Aprendizado de Máquina
19.
Sci Transl Med ; 14(674): eabq6474, 2022 12 07.
Artigo em Inglês | MEDLINE | ID: mdl-36475906

RESUMO

Green light exposure has been shown to reduce pain in animal models. Here, we report a vision-associated enkephalinergic neural circuit responsible for green light-mediated analgesia. Full-field green light exposure at an intensity of 10 lux produced analgesic effects in healthy mice and in a model of arthrosis. Ablation of cone photoreceptors completely inhibited the analgesic effect, whereas rod ablation only partially reduced pain relief. The analgesic effect was not modulated by the ablation of intrinsically photosensitive retinal ganglion cells (ipRGCs), which are atypical photoreceptors that control various nonvisual effects of light. Inhibition of the retino-ventrolateral geniculate nucleus (vLGN) pathway completely abolished the analgesic effects. Activation of this pathway reduced nociceptive behavioral responses; such activation was blocked by the inhibition of proenkephalin (Penk)-positive neurons in the vLGN (vLGNPenk). Moreover, green light analgesia was prevented by knockdown of Penk in the vLGN or by ablation of vLGNPenk neurons. In addition, activation of the projections from vLGNPenk neurons to the dorsal raphe nucleus (DRN) was sufficient to suppress nociceptive behaviors, whereas its inhibition abolished the green light analgesia. Our findings indicate that cone-dominated retinal inputs mediated green light analgesia through the vLGNPenk-DRN pathway and suggest that this signaling pathway could be exploited for reducing pain.


Assuntos
Corpos Geniculados , Manejo da Dor , Camundongos , Animais , Dor
20.
Sci Bull (Beijing) ; 67(7): 733-747, 2022 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-36546138

RESUMO

Diabetes is caused by the interplay between genetics and environmental factors, tightly linked to lifestyle and dietary patterns. In this study, we explored the effectiveness of intermittent protein restriction (IPR) in diabetes control. IPR drastically reduced hyperglycemia in both streptozotocin-treated and leptin receptor-deficient db/db mouse models. IPR improved the number, proliferation, and function of ß cells in pancreatic islets. IPR reduced glucose production in the liver and elevated insulin signaling in the skeletal muscle. IPR elevated serum level of FGF21, and deletion of the Fgf21 gene in the liver abrogated the hypoglycemic effect of IPR without affecting ß cells. IPR caused less lipid accumulation and damage in the liver than that caused by continuous protein restriction in streptozotocin-treated mice. Single-cell RNA sequencing using mouse islets revealed that IPR reversed diabetes-associated ß cell reduction and immune cell accumulation. As IPR is not based on calorie restriction and is highly effective in glycemic control and ß cell protection, it has promising translational potential in the future.


Assuntos
Diabetes Mellitus Experimental , Ilhotas Pancreáticas , Camundongos , Animais , Diabetes Mellitus Experimental/metabolismo , Dieta com Restrição de Proteínas , Estreptozocina/metabolismo , Glucose/metabolismo , Homeostase
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