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1.
Poult Sci ; 99(5): 2543-2554, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32359590

RESUMO

The metabolites of stored, chilled chicken meat were analyzed using liquid chromatograph-mass spectrometry and metabolomics. The results showed significant differences in the metabolites of chicken meat stored at 4°C for 0 D and meat stored for longer periods of 2 D, 4 D, 6 D, and 10 D, when analyzed based on a variable of importance >2 and P < 0.05. These changed metabolites included amino acids, amines, nucleosides, nucleotides, carbohydrates, organic acids, and other substances. The data from this study provide a holistic understanding of food quality changes in chicken meat during deterioration in storage.

2.
J Gastrointest Surg ; 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32410176

RESUMO

PURPOSE: We have shown that activation of the receptor tyrosine kinase (RTK)-RAS pathway in gastric adenocarcinoma (GA) promotes acquisition of cancer stem-like cell (CSC) phenotypes including metastasis and chemotherapy resistance. Here, we evaluated the prognostic value of the CSC marker CD44 and the RTK-RAS activation marker phosphorylated MEK (p-MEK) in patients with resectable GA. METHODS: CD44 and p-MEK were measured in tumors from GA patients who underwent curative-intent gastrectomy at Fujian Medical University Union Hospital (FMUUH, n = 134) and Memorial Sloan Kettering Cancer Center (MSKCC, n = 56). Overall survival (OS) was estimated by the Kaplan-Meier method, and multivariate analysis was performed by Cox proportional hazards regression modeling. RESULTS: Despite multiple significant differences in clinicopathologic characteristics between the FMUUH and MSKCC cohorts, high CD44 and high p-MEK expression were both independent negative prognostic factors for OS on univariate analysis in both cohorts (p < 0.05). Both factors were also significant on multivariate analysis when the cohorts were combined (p ≤ 0.003). On subgroup analysis, the 5-year OS of patients with both high CD44 and high p-MEK was 39.5-41.6% compared with 55.4-66.4% for patients with low CD44. High CD44 expression was associated with more advanced TNM stage in the FMUUH cohort and larger tumor size and undifferentiated histology in the MSKCC cohort. High p-MEK was associated with undifferentiated histology in the FMUUH cohort and larger tumor size in the MSKCC cohort. CONCLUSIONS: Increased CD44 and p-MEK expression are predictive of worse OS in GA patients. Thus, targeting the RTK-RAS pathway may benefit patients with CD44-positive, RAS-activated GA by inhibiting metastasis and reversing chemotherapy resistance.

3.
Psychiatry Investig ; 2020 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-32375461

RESUMO

OBJECTIVE: To systematically evaluate evidence for the use of interventions based on appied behavior analysis (ABA) to manage various symptoms of children with autism spectrum disorder (ASD). METHODS: Sensitivity analyses were conducted by removing any outlying studies and subgroup analyses were performed to compare the effectiveness of ABA and early start denver model (ESDM), picture exchange communication systems (PECS) and discrete trial training (DTT). RESULTS: 14 randomized control trials of 555 participants were included in this meta-analysis. The overall standardized mean difference was d=-0.36 (95% CI -1.31, 0.58; Z=0.75, p=0.45) for autism general symptoms, d=0.11 (95% CI -0.31, 0.54; Z=0.52, p=0.60) for socialization, d=0.30 (95% CI -0.02, 0.61; Z=1.84, p=0.07) for communication and d=-3.52 (95% CI -6.31, -0.72; Z=2.47, p=0.01) for expressive language, d=-0.04 (95% CI -0.44, 0.36; Z=0.20, p=0.84) for receptive language. Those results suggested outcomes of socialization, communication and expressive language may be promising targets for ABA-based interventions involving children with ASD. However, significant effects for the outcomes of autism general symptoms, receptive language, adaptive behavior, daily living skills, IQ, verbal IQ, nenverbal IQ, restricted and repetitive behavior, motor and cognition were not observed. CONCLUSION: The small number of studies included in the present study limited the ability to make inferences when comparing ABA, ESDM, PECS and DTT interventions for children with ASD.

4.
Cell Cycle ; : 1-11, 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32378978

RESUMO

Osteoarthritis (OA) is characterized by apoptosis of chondrocytes and an imbalance of extracellular matrix (ECM) synthesis and catabolism. Emerging evidence has demonstrated that miRNAs are involved in OA pathologies, but the role of miR-296-5p in OA remains unclear. The present study proposes to reveal the functions and mechanisms of miR-296-5p in a cell model of OA. In this study, human chondrocytes were treated with 5 ml interleukin-1 beta (IL-1ß) to induce apoptosis and cartilage degradation. Our results showed that miR-296-5p was downregulated in chondrocytes stimulated with IL-1ß. Overexpressed miR-296-5p enhanced cell proliferation and inhibited apoptosis and matrix degrading enzyme expression in response to IL-1ß stimulation, and knockdown of miR-296-5p showed the opposite effect. Further, we found that miR-296-5p directly targeted the 3'-untranslated region (3'-UTR) of TGF-ß1 mRNA, and miR-296-5p inactivated the TGF-ß1/CTGF/p38MAPK signaling pathway. Overexpression of TGF-ß1 alleviated the inhibition of miR-296-5p on chondrocyte apoptosis and cartilage degradation. In conclusion, miR-296-5p inhibited the progression of OA through the CTGF/p38MAPK pathway by directly targeting TGF-ß1.

5.
Org Biomol Chem ; 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32379263

RESUMO

Spiroconyone A (1), the first rearranged phytosterol featuring an unusual spiro [5,6] ring system, and nine known compounds (2-10) were isolated from the aerial parts of Conyza japonica. The structure of 1 was elucidated through spectroscopic methods, and its absolute configuration was determined by single-crystal X-ray diffraction analysis. Enzyme-based assay revealed that spiroconyone A showed weak TDP1 inhibition and compounds 7 and 10 showed TDP1 inhibition with IC50 values of 36 µM and 16 µM, respectively. MTT assay indicated that 7 and 10 showed a strong synergistic effect with the clinical TOP1 inhibitor topotecan in MCF-7 cells. Compound 5 displayed the most potent cytotoxicity against MCF-7 cells with a GI50 value of 3.3 µM. Furthermore, a hypothetical biosynthetic pathway for 1 was proposed. This work provides valuable information that the secondary metabolites from Conyza japonica could be developed as anticancer agents.

6.
Artigo em Inglês | MEDLINE | ID: mdl-32385341

RESUMO

To define the efficacy of a single dose of 375 mg/m2 rituximab for DSA-positive patients with 2000 ≤ MFI < 10,000, we enrolled a prospective clinical cohort including patients with positive DSA treated with rituximab (n = 55, cohort A), a matched-pair cohort including cases with negative DSA (n = 110, cohort B) and a historical cohort including subjects with 2000 ≤ MFI < 10,000 without receiving any treatment for DSA (n = 22, cohort C). The incidences of primary poor graft function (PGF) in cohort A and cohort B were 5% and 1% (P = 0.076), respectively, both of which were lower than that in cohort C (27%, P < 0.001, for all). Rituximab was associated with a reduced incidence of primary PGF (HR 0.200, P = 0.023). The 3-year nonrelapse mortality of patients in cohort A and cohort B were 23% and 24%, respectively, both of which were lower than that in the cohort C (37%), although no statistical significance was observed. These results led to a low 3-year overall survival in patients in the cohort C (58%) compared with those in the cohort A (71%) and the cohort B (73%). We suggest that a single dose of rituximab could be effectively used to prevent the onset of primary PGF. The prospective cohort of this study is registered at http://www.chictr.org.cn/ChiCTR-OPC-15006672.

7.
Theranostics ; 10(12): 5641-5648, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32373237

RESUMO

Rationale: Chest computed tomography (CT) has been used for the coronavirus disease 2019 (COVID-19) monitoring. However, the imaging risk factors for poor clinical outcomes remain unclear. In this study, we aimed to assess the imaging characteristics and risk factors associated with adverse composite endpoints in patients with COVID-19 pneumonia. Methods: This retrospective cohort study enrolled patients with laboratory-confirmed COVID-19 from 24 designated hospitals in Jiangsu province, China, between 10 January and 18 February 2020. Clinical and initial CT findings at admission were extracted from medical records. Patients aged < 18 years or without available clinical or CT records were excluded. The composite endpoints were admission to ICU, acute respiratory failure occurrence, or shock during hospitalization. The volume, density, and location of lesions, including ground-glass opacity (GGO) and consolidation, were quantitatively analyzed in each patient. Multivariable logistic regression models were used to identify the risk factors among age and CT parameters associated with the composite endpoints. Results: In this study, 625 laboratory-confirmed COVID-19 patients were enrolled; among them, 179 patients without an initial CT at admission and 25 patients aged < 18 years old were excluded and 421 patients were included in analysis. The median age was 48.0 years and the male proportion was 53% (224/421). During the follow-up period, 64 (15%) patients had a composite endpoint. There was an association of older age (odds ratio [OR], 1.04; 95% confidence interval [CI]: 1.01-1.06; P = 0.003), larger consolidation lesions in the upper lung (Right: OR, 1.13; 95%CI: 1.03-1.25, P =0.01; Left: OR,1.15; 95%CI: 1.01-1.32; P = 0.04) with increased odds of adverse endpoints. Conclusion: There was an association of older age and larger consolidation in upper lungs on admission with higher odds of poor outcomes in patients with COVID-19.


Assuntos
Infecções por Coronavirus/diagnóstico por imagem , Pulmão/diagnóstico por imagem , Pulmão/patologia , Pneumonia Viral/diagnóstico por imagem , Adulto , Fatores Etários , Idoso , Algoritmos , Betacoronavirus , China , Infecções por Coronavirus/patologia , Aprendizado Profundo , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/patologia , Prognóstico , Estudos Retrospectivos , Tomografia Computadorizada por Raios X
8.
Lupus ; : 961203320922301, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32356674

RESUMO

INTRODUCTION: The present study aimed to investigate the prevalence and influential factors of thrombocytopaenia in systemic lupus erythematosus (SLE) patients among the Chinese population in order to provide evidence for improving the treatment and nursing of SLE patients. METHODS: A retrospective analysis of 3140 SLE patients admitted to two large tertiary hospitals was conducted in Anhui, China, from 2011 to 2018. In addition, the influential factors related to SLE with thrombocytopaenia were analysed through univariate and multivariate analysis. RESULTS: A total of 804 SLE patients had thrombocytopaenia (25.6%). The top 5 clinical manifestations of SLE inpatients were proteinuria (51.0%), lupus nephritis (45.9%), new rash (38.4%), haematuria (36.7%) and pyuria (32.2%). The incidence of neurological manifestations, oral mucosal ulceration, pleurisy, pericarditis, hyperglycaemia, leucocytopaenia, urinary casts, haematuria, pyuria and high disease activity in the thrombocytopaenia group were higher than those in the non-thrombocytopaenia group (p < 0.05). Multivariate analysis showed age (odds ratio (OR) = 1.009, p = 0.005), neurological manifestations (OR = 1.373, p = 0.048), pericarditis (OR = 1.394, p = 0.048), hyperglycaemia (OR = 1.717, p < 0.001), leucocytopaenia (OR = 2.551, p < 0.001), haematuria (OR = 1.582, p < 0.001), serum C3 level <0.85 g/L (OR = 1.525, p = 0.001), serum C4 concentration <0.10 g/L (OR = 1.287, p = 0.020), serum CRP concentration <8 ng/L (OR = 1.314, p = 0.005), prothrombin time >15.30 seconds (OR = 1.479, p = 0.032), activated partial thromboplatin time >45 seconds (OR = 1.924, p < 0.001) and thrombin time >21 seconds (OR = 1.629, p = 0.015) were associated with thrombocytopaenia. CONCLUSION: Thrombocytopaenia has a high prevalence in SLE patients and is related to some baseline, clinical and laboratory characteristics, affecting multiple organs and systems.

10.
Eur J Obstet Gynecol Reprod Biol ; 250: 17-23, 2020 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-32375083

RESUMO

OBJECTIVES: Healthy embryos and endometrial receptivity are keys to the success of in-vitro fertilization and embryo transfer (IVF-ET). Abnormal transvaginal ultrasonography (TVU) findings in the endometrium are a concern during assisted reproductive technology (ART) treatments. Observation of a hyperechogenic endometrial mass (HEM) during controlled ovarian stimulation (COS) in an IVF or intracytoplasmic sperm injection (ICSI) cycle is a dilemma. Here we investigated whether an HEM would affect ART cycle outcomes. STUDY DESIGN: We analyzed all data from patients who underwent IVF or ICSI treatment cycles from January 1, 2009 to December 31, 2016 at our institution. Patients diagnosed with an HEM were included. Each woman was matched with two control subjects of similar age (± 1 year), in terms of the number of cycles, type of infertility (primary or secondary), protocol used for COS, but with no HEM identified by TVU. Baseline characteristics, COS response, and pregnancy outcomes after ET were compared. RESULTS: In all, 1088 patients were diagnosed with an HEM. Of these, 426 met the inclusion criteria and were matched with 852 controls. Baseline characteristics showed no significant differences between the groups. The peak endometrial thickness was significantly greater in the experimental group than in the control group (1.15 ± 0.18 cm vs. 1.08 ± 0.21 cm, p < 0.001). The live birth rates were 43.7% vs 46.2% in the experimental and control groups, respectively. There were no significant between-group differences in the rates of biochemical or clinical pregnancy or abortions at any stage. CONCLUSIONS: Newly diagnosed HEMs < 18 mm in diameter monitored by TVU did not impair the outcomes of ART following COS in this study. If other conditions are suitable, completion of ART cycles should be the appropriate approach in such cases. KEY MESSAGE: Newly diagnosed hyperechogenic endometrial masses measuring <18 mm in diameter monitored by TVU during COS did not adversely affect the outcomes of these IVF/ICSI-ET cycles.

12.
Zhen Ci Yan Jiu ; 45(4): 305-9, 2020 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-32333536

RESUMO

OBJECTIVE: To observe the effect of moxibustion and acupoint catgut embedding (ACE) at "Tianshu"(ST25) "Dachangshu"(BL25) and "Shangjuxu"(ST37) on changes of body mass, stool property, histopathological conditions and expression levels of interleukin 6 (IL-6) in colonic mucosa of ulcerative colitis (UC) rats, so as to reveal its anti-inflammatory mechanisms underlying improvement of UC. METHODS: SD rats were randomized into normal, model, moxibustion, ACE and moxibustion+ACE groups (n=6 in each group). The UC model was established by enema of trinitro-benzene-sulfonic acid and ethanol. Moxibustion was applied to bilateral ST25, BL25 and ST37 for 10 min, once daily for 14 days, and ACE applied to the same 3 acupoints, once a week for two weeks. After the treatment, the rats' general conditions were observed, and the severity of UC was assessed by using disease activity index (DAI) score. Colonic mucosal pathological changes were observed under microscope after hematoxylin eosin (H.E.) stain, and the expression levels of IL-6 in the colonic mucosa tissue detected by using immunohistochemical stain and Western blot, respectively. RESULTS: After modeling, the DAI score, and expression level of colonic IL-6 protein detected by immunohistochemistry and Western blot were obviously increased in the model group relevant to the normal group (P<0.01). Following the intervention, the increase of DAI score and IL-6 expression were reversed in moxibustion, ACE and moxibustion+ACE groups (P<0.01, P<0.05). The therapeutic effects of moxibustion+ACE were considerably superior to those of simple ACE and simple moxibustion in down-regulating the levels of DAI score and IL-6 expression (P<0.01). H.E. staining showed severe defect of the colonic mucosal epithelium with infiltration of a large number of inflammatory cells in the model group, which was milder in moxibustion, ACE and moxibustion+ACE groups. CONCLUSION: Moxibustion combined with ACE is able to improve the inflammatory injury of colonic mucosa in UC rats, which may be related with its effect in suppressing the expression of colonic IL-6; and the efficacy of moxibustion+ACE is apparently superior to that of moxibustion and ACE alone.

13.
Artigo em Inglês | MEDLINE | ID: mdl-32335836

RESUMO

In this study, the mud-phosphorus slurry was used to simultaneously remove SO2 and NOx. The technology proposed new avenues for the purification and utilization of remove SO2 and NOx in flue gas. The effects of reaction temperature, solid-liquid ratio, and oxygen content on the efficiency of desulfurization and denitrification were studied experimentally. Results show that the parameters were solid-liquid ratio of 5.0 g/40 mL, T = 60 °C, φ (O2) = 20%, Q = 300 mL/min under the best experimental conditions. The maximum amount of ozone generated was 563.8 mg/m3. The reaction time with desulfurization rate ≥ 99% was 340 min; the reaction time with denitrification rate ≥ 99% was 160 min. Response surface analysis method was used to perform a three-factor three-level response surface experiment. Results show that the oxygen content had a highly significant effect on the desulfurization and denitrification efficiency, and the relationship between the desulfurization and denitrification efficiency was oxygen content > mud-phosphorus slurry liquid-solid ratio > reaction temperature. The process is simple, the solid waste is used to treat the flue gas, and the removal effect is good, which is convenient for popularization.

14.
Mol Plant Pathol ; 21(6): 749-760, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32319186

RESUMO

Grey mould is one of the most determinative factors of lily growth and plays a major role in limiting lily productivity. MicroRNA159 (miR159) is a highly conserved microRNA in plants, and participates in the regulation of plant development and stress responses. Our previous studies revealed that lre-miR159a participates in the response of Lilium regale to Botrytis elliptica according to deep sequencing analyses; however, the response mechanism remains unknown. Here, lre-miR159a and its target LrGAMYB gene were isolated from L. regale. Transgenic Arabidopsis overexpressing lre-MIR159a exhibited larger leaves and smaller necrotic spots on inoculation with Botrytis than those of wild-type and overexpressing LrGAMYB plants. The lre-MIR159a overexpression also led to repressed expression of two targets of miR159, AtMYB33 and AtMYB65, and enhanced accumulation of hormone-related genes, including AtPR1, AtPR2, AtNPR1, AtPDF1.2, and AtLOX for both the jasmonic acid and salicylic acid pathways. Moreover, lower levels of H2 O2 and O 2 - were observed in lre-MIR159a transgenic Arabidopsis, which reduced the damage from reactive oxygen species accumulation. Taken together, these results indicate that lre-miR159a positively regulates resistance to grey mould by repressing the expression of its target LrGAMYB gene and activating a defence response.

15.
Artigo em Inglês | MEDLINE | ID: mdl-32225026

RESUMO

PURPOSE: The purpose of this study was to investigate the influence of natural environmental factors on the spatial distribution of marathon events in China, and to identify the suitable natural environmental factors for the marathon events. METHODS: Geographic information system (GIS) spatial analysis tools were used to perform coupling analysis, e.g. overlap, neighborhood, intersection and buffer for terrain, climate, air quality, mountains and water resources with 342 marathon events held in China in 2018. RESULTS: The results indicate that the spatial distribution of marathon events in China is negatively correlated with the elevation of the terrain (plain > hill > plateau > mountain > basin); climate (subtropical monsoon climate > temperate monsoon climate > temperate continental climate > tropical monsoon climate > plateau alpine climate), air quality (level 3 > level 2 > level 4 > level 1). Results indicate that buffer zones can protect water resources: there are 24 items in the buffer zone of river 0.5 km and lake 1 km, 131 items in the buffer zone of river 3 km and lake 5 km, 191 items in the buffer zone of river 5 km and lake 10 km, 298 items in the buffer zone of river 10 km and lake 20 km. Results indicate for mountain range buffer: 13 items in the 20 km buffer and 39 items in the 50 km buffer. CONCLUSIONS: Marathon events are more likely to be held on the third rung of China's topography where a city has a typical landform (plains, basins, hills, or mountain) with good climate and air quality. Meanwhile a city with water and mountain resources for recreational events such as cross-country or obstacle course are essential. The contribution of this study is to systematically and intuitively reflect the influence of natural environment factors on the distribution of marathon events in China, and to provide evidence for the medium and long-term planning of marathon events in China, the selection of venues for different types of marathon events and how to attract participants.

16.
Am J Health Behav ; 44(3): 326-332, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32295680

RESUMO

Objective: Sustained attention is critical for various activities of daily living, including engaging in health-enhancing behaviors and inhibition of health compromising behaviors. Sustained attention activates neural networks involved in episodic memory function, a critical cognition for healthy living. Acute exercise has been shown to activate these same neural networks. Thus, it is plausible that engaging in a sustained attention task and engaging in a bout of acute exercise may have an additive effect in enhancing memory function, which was the purpose of this experiment. Methods: 23 young adults (Mage = 20.7 years) completed 2 visits, with each visit occurring approximately 24 hours apart, in a counterbalanced order, including: (1) acute exercise with sustained attention, and (2) sustained attention only. Memory was assessed using a word-list paradigm and included a short- and long-term memory assessment. Sustained attention was induced via a sustained attention to response task (SART). Acute exercise involved a 15-minute bout of moderate-intensity exercise. Results: Short-term memory performance was significantly greater than long-term memory, Mdiff = 1.86, p < .001, and short-term memory for Exercise with Sustained Attention was significantly greater than short-term memory for Sustained Attention Only, Mdiff = 1.50, p = .01. Conclusion: Engaging in an acute bout of exercise before a sustained attention task additively influenced short-term memory function.

17.
Medicine (Baltimore) ; 99(14): e19548, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32243371

RESUMO

BACKGROUND: Essential hypertension is a multifactorial disease, which is affected by genetic and environmental factors, and can cause diseases such as cerebrovascular disease, heart failure, coronary heart disease, and chronic renal failure. High salt intake is a risk factor for hypertension, stroke, and cardiovascular disease. Blood pressure variability (BPV) is a reliable independent predictor of cardiovascular events and death. At present, there are few studies about the correlation among high salt intake, BPV, and target organ damage (TOD) in patients with hypertension. OBJECTIVE: The purpose of this study is to compare 24-hour urine sodium excretion, BPV, carotid intima-media thickness, left ventricular mass index, and serum creatinine or endogenous creatinine clearance rate. To clarify the relationship between high salt load and BPV and TOD in patients with hypertension.This study is a cross-sectional study. It will recruit 600 patients with essential hypertension in the outpatient and inpatient department of cardiovascular medicine of Chengdu Fifth People's Hospital. Researchers will obtain blood and urine samples with the patient's informed consent. In addition, we will measure patient's blood pressure and target organ-related information. TRIAL REGISTRY: The study protocol was approved by the Chengdu Fifth People's Hospital. Written informed consent will be obtained from all the participants. The trial was registered in the Chinese Clinical trial registry, ChiCTR2000029243. This trial will provide for the correlation among high salt intake, BPV, and TOD in patients with essential hypertension.


Assuntos
Pressão Sanguínea/fisiologia , Espessura Intima-Media Carotídea , Hipertensão Essencial/fisiopatologia , Hipertrofia Ventricular Esquerda/patologia , Cloreto de Sódio na Dieta/urina , Fatores Etários , Pesos e Medidas Corporais , Comorbidade , Creatinina/sangue , Estudos Transversais , Hipertensão Essencial/urina , Feminino , Comportamentos Relacionados com a Saúde , Humanos , Masculino , Fatores Sexuais
18.
J Immunol ; 204(10): 2712-2721, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32269097

RESUMO

C-reactive protein (CRP) can increase up to 1000-fold in blood and form complexes with very low density lipoproteins (VLDL). These complexes are associated with worse outcomes for septic patients, and this suggests a potential pathological role in sepsis. Complex formation is heightened when CRP is over 200 mg/l and levels are associated with the severity of sepsis and blood bacterial culture positivity. Using a mouse bacteremia model, blood bacterial clearance can be delayed by i.v. injection of CRP-VLDL complexes. Complexes are more efficiently taken up by activated U937 cells in vitro and Kupffer cells in vivo than VLDL alone. Both in vitro-generated and naturally occurring CRP-VLDL complexes reduce phagocytosis of bacteria by activated U937 cells. Fcγ and scavenger receptors are involved and a competitive mechanism for clearance of CRP-VLDL complexes and bacteria is demonstrated. Interaction of phosphocholine groups on VLDL with CRP is the major driver for complex formation and phosphocholine can disrupt the complexes to reverse their inhibitory effects on phagocytosis and bacterial clearance. Increased formation of CRP-VLDL complexes is therefore harmful and could be a novel target for therapy in sepsis.

19.
J Phys Chem A ; 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32286070

RESUMO

It is well known that imidazoles, possessing two or more nitro substituents, are potential candidates for highly energetic explosives with detonation parameters comparable to those of 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX) and 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane. 4,4',5,5'-Tetranitro-2,2'-bi-imidazole (TNBI) is a typical imidazole explosive with energy equivalent to that of RDX but suffers from low sensitivity (impact sensitivity 7 J). 1,1'-Diamino-4,4',5,5'-tetranitro-2,2'-biimidazole (DATNBI), a derivative of TNBI, possesses two -NH2 groups and has a higher detonation velocity (9063 m s-1) and lower impact sensitivity of 15 J, which indicates great potential for future applications. Examination of the thermal decomposition mechanism and kinetics of TNBI and DATNBI gives a more comprehensive view of the influence that the -NH2 group has on the sensitivity and storage safety of the energetic explosive-based TNBI molecular skeleton. Herein, the thermal decomposition mechanism is studied, showing that detachment of -NH2 groups from DATNBI generates 1-diamino-4,4',5,5'-tetranitro-2,2'-biimidazole (ATNBI) and TNBI and induces self-decomposition. Although the decomposition peak temperature of DATNBI is significantly lower than that of TNBI at the same heating rate; its self-accelerating decomposition temperature (50 kg) is only 4 K lower. Therefore, the -NH2 group displays good ability of reducing sensitivity but has no influence on storage safety of DATNBI.

20.
Cytometry A ; 2020 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-32307867

RESUMO

We examined our hypothesis that high-intensity focused ultrasound (HIFU) treatment of pancreatic ductal adenocarcinoma (PDAC) in nude mice models may lead to an increased occurrence of hematogenous metastasis. The human PDAC cell line BxPC-3 transfected with mCherry was implanted into nude mice to establish orthotopic and subcutaneous xenograft (OX and SX) tumor models. Mice were exposed to HIFU when tumor sizes reached approximately 200-300 mm3 . The OX and SX tumor models were monitored continuously for tumor growth characteristics and hematogenous metastasis using in vivo flow cytometric (IVFC) detection of circulating tumor cells (CTCs) from the pancreas. We chose an appropriate mouse model to further examine whether or not HIFU increases the potential risk of hematogenous metastasis, using IVFC detection. Our results showed that the CTC number was greater in the OX model than in the SX model. The CTC number in the OX model increased gradually over time, whereas the CTC number in the SX model remained low. Therefore, the OX model was better for studying tumor metastasis by IVFC detection. We found significantly decreased CTC numbers and tumor volume after HIFU ablation. Our results showed the applicability of the PDAC OX tumor model for studying the occurrence of tumor metastasis due to the generation of CTCs. HIFU ablation substantially restricted PDAC hematogenous metastasis and provided effective tumor control locally. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals Inc., on behalf of International Society for Advancement of Cytometry.

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