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1.
Microb Pathog ; 161(Pt A): 105230, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34619313

RESUMO

Sallmonella Pullorum is a host-restricted pathogen for poultry and causes severe economic importance in many developing countries. The development of novel vaccines for Salmonella Pullorum is necessary to eradicate the prevalence of the pathogen. In our study, a srfA deletion mutant (C79-13ΔsrfA) of Salmonella Pullorum was constructed, and then the biological characteristics and protective efficacy of the mutant were evaluated. The mutant C79-13ΔsrfA was much less virulent than its parental strain C79-13 in one-day-old HY-line white chickens, immunization with C79-13ΔsrfA (4 × 107 CFU) through oral pathway induced highly specific humoral and cellular immune responses, the growth performance of vaccinated chickens was consistent with that of unvaccinated chickens. The survival percentages of vaccinated chickens reached 90% and 80%, after challenge with Salmonella Pullorum strain C79-13 and Salmonella Gallinarum strain SG9 at 10 days post-immunization (dpi), respectively. Collectively, our results indicate that C79-13ΔsrfA is a live attenuated vaccine candidate.

2.
Phys Rev Lett ; 127(12): 127001, 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34597086

RESUMO

Recent measurements of the resistivity in magic-angle twisted bilayer graphene near the superconducting transition temperature show twofold anisotropy, or nematicity, when changing the direction of an in-plane magnetic field [Cao et al., Science 372, 264 (2021)SCIEAS0036-807510.1126/science.abc2836]. This was interpreted as strong evidence for exotic nematic superconductivity instead of the widely proposed chiral superconductivity. Counterintuitively, we demonstrate that in two-dimensional chiral superconductors the in-plane magnetic field can hybridize the two chiral superconducting order parameters to induce a phase that shows nematicity in the transport response. Its paraconductivity is modulated as cos(2θ_{B}), with θ_{B} being the direction of the in-plane magnetic field, consistent with experiment in twisted bilayer graphene. We therefore suggest that the nematic response reported by Cao et al. does not rule out a chiral superconducting ground state.

3.
Chem Biol Interact ; 349: 109683, 2021 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-34610339

RESUMO

Previous studies have shown that PPP1R13L as an inhibitor of apoptosis protease TP53 can lead to abnormal cell proliferation and carcinogenesis, however, the function of PPP1R13L was complicated and the interaction between TP53 and PPP1R13L needs to be further explored. In the present study, a malignant transformation model of human bronchial epithelial cells induced by benzo (a) pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE) was established to observe the regulatory patterns between TP53 and PPP1R13L during carcinogenesis. In vitro experiments including CRISPR-Cas9 editing, RNA silence, Co-Immunoprecipitation and Chromatin Immunoprecipitation were applied to discuss their interactive effects. Additionally, TCGA data profile and our clinical samples of lung cancer were also used to analyze their relationship at the transcriptome level. Interestingly, we found that the mRNA and protein level of TP53 and PPP1R13L fluctuated as a wave in BPDE-induced malignant transformation under wild-type TP53 genetic background. Our results have also demonstrated that PPP1R13L acts as an inhibitor of TP53, while TP53 can regulate PPP1R13L via binding a possible enhancer of the first intron of PPP1R13L gene. Likewise, TCGA data and clinical samples have identified that in the case of TP53 mutation, TP53 expression was negatively correlated with PPP1R13L, while in the case of TP53 wild-type, TP53 expression was not correlated with PPP1R13L. It suggested that there existed a negative feedback of wild-type TP53 to PPP1R13L, which reminded a unique implication during chemical carcinogenesis.

4.
5.
Artigo em Inglês | MEDLINE | ID: mdl-34623517

RESUMO

PURPOSE: This study aimed to investigate the efficacy of digital mammography (DM), digital breast tomosynthesis (DBT), diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) MRI separately and combined in the prediction of molecular subtypes of breast cancer. METHODS: A total of 241 patients were enrolled and underwent breast MD, DBT, DW and DCE scans. Radiomics features were calculated from intra- and peritumoral regions, and selected with least absolute shrinkage and selection operator (LASSO) regression to develop radiomics signatures (RSs). Prediction performance of intra- and peritumoral regions in the four modalities were evaluated and compared with area under the receiver-operating characteristic (ROC) curve (AUC), specificity and sensitivity as comparison metrics. RESULTS: The RSs derived from combined intra- and peritumoral regions improved prediction AUCs compared with those from intra- or peritumoral regions alone. DM plus DBT generated better AUCs than the DW plus DCE on predicting Luminal A and Luminal B in the training (Luminal A: 0.859 and 0.805; Luminal B: 0.773 and 0.747) and validation (Luminal A: 0.906 and 0.853; Luminal B: 0.807 and 0.784) cohort. For the prediction of HER2-enriched and TN, the DW plus DCE yielded better AUCs than the DM plus DBT in the training (HER2-enriched: 0.954 and 0.857; TN: 0.877 and 0.802) and validation (HER2-enriched: 0.974 and 0.907; TN: 0.938 and 0.874) cohort. CONCLUSIONS: Peritumoral regions can provide complementary information to intratumoral regions for the prediction of molecular subtypes. Compared with MRI, the mammography showed higher AUCs for the prediction of Luminal A and B, but lower AUCs for the prediction of HER2-enriched and TN.

6.
Transl Psychiatry ; 11(1): 505, 2021 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-34601497

RESUMO

Close contacts of those with COVID-19 (CC) may experience distress and long-lasting mental health effects. However, the mental health status and quality of life (QOL) in CC have not been adequately examined. This study examined the mental health status and QOL in CC during the post-COVID-19 period. This cross-sectional study comprised 1169 CC and 1290 who were non-close contacts (non-CC). Demographic data were collected; depression, fatigue, post-traumatic stress symptoms (PTSS) and QOL were assessed using the Patient Health Questionnaire - 9 items (PHQ-9), fatigue numeric rating scale, Post-Traumatic Stress Disorder Checklist - 17 items (PCL-17), and the World Health Organization Quality of Life Questionnaire - brief version (WHOQOL-BREF), respectively. Analysis of covariance was used to compare depressive symptoms, QOL, fatigue, and PTSS between the CC and non-CC groups. Multiple logistic regression analyses were performed to determine the independent correlates for depression, fatigue, PTSS, and QOL in the CC group. Compared to the non-CC group, the CC group reported significantly more severe depression (F(1, 2458) = 5.58, p = 0.018) and fatigue (F(1, 2458) = 9.22, p = 0.002) in the post-COVID-19 period. No significant differences in PTSS and QOL between the CC and non-CC groups were found (F(1, 2458) = 2.93, p = 0.087 for PTSS; F(1, 2458) = 3.45, p = 0.064 for QOL). In the CC group, younger age, financial loss due to COVID-19, and perception of poor or fair health status were significantly associated with depression and fatigue, while frequent use of mass media was significantly associated with fatigue. In conclusion, close contacts of COVID-19 patients experienced high levels of depression and fatigue in the post-COVID-19 period. Due to the negative effects of depression and fatigue on daily functioning, early detection and timely interventions should be provided to this neglected population.


Assuntos
COVID-19 , Qualidade de Vida , Estudos Transversais , Depressão , Nível de Saúde , Humanos , SARS-CoV-2 , Inquéritos e Questionários
7.
Sci Total Environ ; : 150868, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34626623

RESUMO

Land degradation has become one of the most critical environmental and socioeconomic issues in the world, particularly in Central Asia. Moreover, the realization of Land Degradation Neutrality (LDN) in Central Asia faces enormous challenges in achieving the global Sustainable Development Goal 15.3 (SDG 15.3). It is critical to monitor land degradation and assess its drivers in Central Asia. In this study, an Optimal Land Degradation Index (OLDI) was established as a new index for monitoring land degradation using a constrained optimization algorithm. The spatiotemporal characteristics of LDN were monitored in Central Asia. Further analysis explored the driving force of land degradation in different areas. The results showed that 7.22% and 15.33% of the total land area exhibited land improvement and land degradation, respectively. According to abrupt change analysis, mutation changes in the OLDI were observed in 2005, 2012 and 2015. At the subnational scale, most regions in Central Asia have not achieved the goal of LDN. The residual analysis highlighted the drivers of spatial differences in land degradation performance in Central Asia. Drought was the main driving force affecting land degradation by the compound effect of decreased precipitation and increased temperature on the Ustyurt Plateau, while 24.01% of the land degradation areas resulted from anthropogenic disturbances and were mainly distributed in the areas surrounding the Aral Sea. The results also indicated that 72.56% of the land improvement areas resulted from human activities and were mainly concentrated in the Balkhash Lake Delta and the Amudarya Delta. In Central Asia, the realization of SDG 15.3 by 2030 remains a severe challenge. Restoration measures should be prioritized in land degradation areas in Central Asia to implement the LDN initiative, especially around the Aral Sea.

8.
Phys Med Biol ; 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34633298

RESUMO

The present study intended to use radiomic analysis of spinal metastasis subregions to detect epidermal growth factor receptor (EGFR) mutation. In total, 94 patients with thoracic spinal metastasis originated from primary lung adenocarcinoma (2017 - 2020). All patients underwent T1-weighted (T1W) and T2 fat-suppressed (T2FS) MRI scans. The spinal metastases (tumor region) were subdivided into phenotypically consistent subregions based on patient- and population-level clustering: Three subregions, S1, S2 and S3, and the total tumor region. Radiomics features were extracted from each subregion and from the whole tumor region as well. Least shrinkage and selection operator (LASSO) regression were used for feature selection and radiomics signature definition. Detection performance of S3 was better than all other regions using T1W (AUCs, S1 vs. S2 vs. S3 vs. whole tumor, 0.720 vs. 0.764 vs. 0.786 vs. 0.758) and T2FS (AUCs, S1 vs. S2 vs. S3 vs. whole tumor, 0.791 vs. 0.708 vs. 0.838 vs. 0.797) MRI. The multi-regional radiomics signature derived from the joint of inner subregion S3 from T1W and T2FS MRI achieved the best detection capabilities with AUCs of 0.879 (ACC = 0.774, SEN = 0.838, SPE = 0.840) and 0.777 (ACC = 0.688, SEN = 0.947, SPE = 0.615) in the training and test sets, respectively. Our study revealed that MRI-based radiomic analysis of spinal metastasis subregions has the potential to detect the EGFR mutation in patients with primary lung adenocarcinoma.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34480655

RESUMO

PURPOSE: Seizure originates from different pathological substrate; however, the same pathologies may have distinct mechanisms underlying seizure generation. We aimed to improve the understanding of such mechanisms in patients with temporal lobe epilepsy (TLE) by investigating the stereoelectroencephalography (SEEG) ictal onset patterns (IOPs). METHODS: We analyzed data from a cohort of 19 consecutive patients explored by SEEG and had 1-3-year seizure-freedom following temporal lobe resection. RESULTS: Six IOPs were identified. They were low voltage fast activity (LVFA) (36.5%), rhythmic spikes or spike-waves at low frequency and with high amplitude (34.1%), runs of spikes (10.6%), rhythmic sharp waves (8.2%), low frequency high amplitude repetitive spiking (LFRS) (7.1%), and delta activity (3.5%). All six patterns were found in patients with mesial temporal onset and only two patterns were found in patients with temporal neocortical onset. The most prevalent patterns for patients with mesial temporal onset were rhythmic spikes or spike-waves, followed by LVFA with a mean discharge rate 74 Hz. For patients with temporal neocortical onset, the most prevalent IOP pattern was LVFA with a mean discharge rate 35 Hz, followed by runs of spikes. Compared with Lateral TLE (LTLE), the duration between the onset of the IOPs to the onset of the symptom was longer for patients with MTLE (Mesial TLE) (MTLE:55.7 ± 50.6 s vs LTLE:19.5 ± 16.4 s). CONCLUSION: Multiple IOPs underlie seizure generation in patients with TLE. However, the mesial and lateral temporal lobes share distinct IOPs.

10.
Stat Med ; 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34474504

RESUMO

In this article, we consider the density estimation for data with a mixture structure, where the component densities are assumed unknown, but for each observation, the probabilities of its membership to the subpopulations are known or estimable from other resources. Data of this kind arise from practice and have wide applications. Motivated from the classical kernel density estimation method for a single population, we propose a weighted kernel density estimation method to estimate the component density functions nonparametrically. Within the framework of the EM algorithm, we derive an algorithm that computes our proposed estimates effectively. Via extensive simulation studies, we demonstrate that our methods outperform the existing methods in most occasions. We further compare our methods with existing methods by real data examples.

11.
Exp Brain Res ; 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34476536

RESUMO

To investigate the association of miR-106b-5p with neuroinflammation and microglial activation in a status epilepticus (SE) mouse model. We examined changes in the expression of microRNA-106b-5p (miRNA-106b-5p), repulsive guidance molecule A (RGMa), triggering receptor expressed on myeloid cells 2 (TREM2), and the microglia-related markers interleukin (IL)-1ß, IL-4, IL-6, IL-10, inducible nitric oxide synthase (iNOS), and arginase-1 (Arg-1) in the mouse hippocampus of the lithium-pilocarpine-induced SE mouse model. Eighty-four female C57BL/6 mice were randomly divided into a normal control group (n = 12), and six SE groups (n = 12/group), which were monitored at 6 h and at 1, 3, 7, 14, and 21 days (d) post-SE induction. Unlike in the dentate gyrus, immunohistochemical staining revealed prominent neuronal swelling at 6 h, significant neuronal loss and apoptosis on day 3, and recovery by day 14 in the hippocampal cornu ammonis (CA)1 and CA3 pyramidal cells in SE mice. We noted elevated levels of miRNA-106b-5p and all microglia-related markers, which peaked at 3 days post-SE, except IL-4, which peaked at 7 days post-SE, indicating inflammation and microglial activation. RGMa and TREM2 levels decreased at 6 h post-SE. All markers but miRNA-106b-5p, RGMa, and TREM2 returned to baseline levels at 21 days post-SE. Dual luciferase reporter gene assay showed that microRNA-106b-5p can interact with RGMa. We observed that miR-106b-5p level increased while both RGMa and TREM2 levels decreased post-SE and showed associations with microglial activation and inflammation in the mouse hippocampus, suggesting their potential as SE therapeutic targets.

12.
Nano Lett ; 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34491061

RESUMO

Perovskite semiconductor nanocrystals are promising for optical amplification and laser applications benefiting from efficient optical gain generation. Nevertheless, the pump threshold is limited by more than one exciton per nanocrystal required to generate population inversion in neutral nanocrystals due to the level degeneracy. Here, we show that by charging nanocrystals with current injection, the level degeneracy can be lifted to generate charged exciton gain with markedly low excitation density. On the basis of the scenario, we have demonstrated electrical switching of amplified spontaneous emission in films of CsPbBr3 nanocrystals sandwiched by two electrodes with over 50% threshold reduction owing to charged excitons. Our work provides an effective approach to electrically modulated optical gain in colloidal perovskite nanocrystals for potential applications in advanced laser and information technology.

13.
Transl Psychiatry ; 11(1): 460, 2021 09 06.
Artigo em Inglês | MEDLINE | ID: mdl-34489416

RESUMO

In network theory depression is conceptualized as a complex network of individual symptoms that influence each other, and central symptoms in the network have the greatest impact on other symptoms. Clinical features of depression are largely determined by sociocultural context. No previous study examined the network structure of depressive symptoms in Hong Kong residents. The aim of this study was to characterize the depressive symptom network structure in a community adult sample in Hong Kong during the COVID-19 pandemic. A total of 11,072 participants were recruited between 24 March and 20 April 2020. Depressive symptoms were measured using the Patient Health Questionnaire-9. The network structure of depressive symptoms was characterized, and indices of "strength", "betweenness", and "closeness" were used to identify symptoms central to the network. Network stability was examined using a case-dropping bootstrap procedure. Guilt, Sad Mood, and Energy symptoms had the highest centrality values. In contrast, Concentration, Suicide, and Sleep had lower centrality values. There were no significant differences in network global strength (p = 0.259), distribution of edge weights (p = 0.73) and individual edge weights (all p values > 0.05 after Holm-Bonferroni corrections) between males and females. Guilt, Sad Mood, and Energy symptoms were central in the depressive symptom network. These central symptoms may be targets for focused treatments and future psychological and neurobiological research to gain novel insight into depression.


Assuntos
COVID-19 , Depressão , Adulto , Estudos Transversais , Depressão/epidemiologia , Feminino , Hong Kong/epidemiologia , Humanos , Masculino , Pandemias , SARS-CoV-2
14.
Artigo em Inglês | MEDLINE | ID: mdl-34529563

RESUMO

In this paper, we propose an efficient method for robust and accurate 3D self-portraits using a single RGBD camera. Our method can generate detailed and realistic 3D self-portraits in seconds and shows the ability to handle subjects wearing extremely loose clothes. To achieve highly efficient and robust reconstruction, we propose PIFusion, which combines learning-based 3D recovery with volumetric non-rigid fusion to generate accurate sparse partial scans of the subject. Meanwhile, a non-rigid volumetric deformation method is proposed to continuously refine the learned shape prior. Moreover, a lightweight bundle adjustment algorithm is proposed to guarantee that all the partial scans can not only loop with each other but also remain consistent with the selected live key observations. Finally, to further generate realistic portraits, we propose non-rigid texture optimization to improve the texture quality. Additionally, we also contribute a benchmark for single-view 3D self-portrait reconstruction, an evaluation dataset that contains 10single-view RGBD sequences of a self-rotated performer wearing various clothes and the corresponding ground-truth 3D models in the first frame of each sequence. The results and experiments based on this dataset show that the proposed method outperforms state-of-the-art methods on accuracy, efficiency, and generality.

15.
Am J Addict ; 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34532935

RESUMO

BACKGROUND AND OBJECTIVES: The prevalence of problematic Internet use (PIU) in the post-COVID-19 pandemic era is not known. This cross-sectional study aimed to determine the prevalence of PIU among baccalaureate nursing students (hereafter: nursing students) in the post-COVID-19 era. METHODS: A total of 1070 nursing students were consecutively invited to participate in this study from the nursing schools of five universities. PIU and quality of life (QOL) were assessed using the Internet Addiction Test (IAT) and the World Health Organization Quality of Life Scale Brief Version (WHOQOL-BREF), respectively. t Tests, χ2 , tests, and Kruskal-Wallis tests were used to compare basic demographic and clinical characteristics between participants with and without PIU. Binary logistic regression analysis was used to examine independent correlates. RESULTS: The prevalence of PIU was 23.3% (95% confidence interval [CI]: 20.7%-25.8%). Multiple logistic regression analysis revealed that second- (p = .024) and third-year (p = .012) students were more likely to suffer from PIU compared with first year students. Students with more severe depressive (p = .014) and anxiety symptoms (p = .011) were independently and significantly associated with more severe PIU. After controlling for covariates, nursing students with PIU had a lower overall QOL score (p = .002). CONCLUSION AND SCIENTIFIC SIGNIFICANCE: Problematic Internet use (PIU) was common among nursing students in the post-COVID-19 era. Considering the negative impact of PIU on QOL and academic performance, regular screening should be conducted and effective interventions implemented for nursing students with PIU. This was the first study on the prevalence of PIU among nursing students in the post-COVID-19 era. The findings of this study could help health professionals and education authorities to understand the patterns of PIU and its influence on QOL among nursing students and to allocate health resources and develop effective measures to reduce the risk of PIU in this population.

16.
Int J Biol Sci ; 17(13): 3413-3427, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34512156

RESUMO

Cardiovascular diseases (CVDs) caused by arteriosclerosis are the leading cause of death and disability worldwide. In the late stages of atherosclerosis, the atherosclerotic plaque gradually expands in the blood vessels, resulting in vascular stenosis. When the unstable plaque ruptures and falls off, it blocks the vessel causing vascular thrombosis, leading to strokes, myocardial infarctions, and a series of other serious diseases that endanger people's lives. Therefore, regulating plaque stability is the main means used to address the high mortality associated with CVDs. The progression of the atherosclerotic plaque is a complex integration of vascular cell apoptosis, lipid metabolism disorders, inflammatory cell infiltration, vascular smooth muscle cell migration, and neovascular infiltration. More recently, emerging evidence has demonstrated that non-coding RNAs (ncRNAs) play a significant role in regulating the pathophysiological process of atherosclerotic plaque formation by affecting the biological functions of the vasculature and its associated cells. The purpose of this paper is to comprehensively review the regulatory mechanisms involved in the susceptibility of atherosclerotic plaque rupture, discuss the limitations of current approaches to treat plaque instability, and highlight the potential clinical value of ncRNAs as novel diagnostic biomarkers and potential therapeutic strategies to improve plaque stability and reduce the risk of major cardiovascular events.

17.
J Colloid Interface Sci ; 607(Pt 1): 769-781, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34536934

RESUMO

In this study, non-noble metal Cd decorated cubic phase CdS (CdS/Cd) thin layer on a millimeter-scale chitosan-Mg(OH)2 xerogel beads (CMB) were elaborately designed and successfully synthesized via facile hydrated electrons (eaq•-) assistant strategy. The in-situ formation of metallic Cd was driven by eaq•- generated from UV/Na2SO3 process. Owing to metallic Cd, CMB@CdS/Cd exhibited better visible-light absorption ability and more efficient separation capability for photo-induced carriers, its hydrogen production efficiency was about threefold improved compared to CMB@CdS. Both characterization methods and density functional theory calculations determined a built-in electric field from metallic Cd to CdS and Ohmic-contact between Cd and CdS, which largely promoted the carriers transfer efficiency. Moreover, the introduction of metallic Cd on the CdS could reduce the ΔGH*, thus greatly boosting the photocatalytic hydrogen production efficiency. This work provides a simple and green approach to construct metallic Cd coupled semiconductor to achieve efficient photocatalytic applications.

18.
Artigo em Inglês | MEDLINE | ID: mdl-34476881

RESUMO

The scarcity of reliable methods for synthesizing chiral gem-diarylmethine borons limits their applications. Herein, we report a method for highly enantioselective dirhodium-catalyzed B-H bond insertion reactions with diaryl diazomethanes as carbene precursors. These reactions afforded chiral gem-diarylmethine borane compounds in high yield (up to 99 % yield), high activity (turnover numbers up to 14 300), high enantioselectivity (up to 99 % ee) and showed unprecedented broad functional group tolerance. The borane compounds synthesized by this method could be efficiently transformed into diaryl methanol, diaryl methyl amine, and triaryl methane derivatives with good stereospecificity. Mechanistic studies suggested that the borane adduct coordinated to the rhodium catalyst and thus interfered with decomposition of the diazomethane, and that insertion of a rhodium carbene (generated from the diaryl diazomethane) into the B-H bond was most likely the rate-determining step.

19.
Clin Neurol Neurosurg ; 209: 106918, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34500340

RESUMO

OBJECTIVES: Vagus nerve stimulation (VNS) has been widely used as an effective treatment for patients with drug-resistant epilepsy (DRE). However, little is known about grey matter (GM) and white matter (WM) microstructure changes caused by VNS. This study aimed to detect consistent GM and WM alterations in epilepsy patients with vagus nerve stimulators. METHODS: The diffusion tensor imaging data was acquired from 15 patients who underwent VNS implantation. The voxel-based morphometry (VBM) and tract-based spatial statistics (TBSS) were used to detect group differences in GM and WM microstructure and explore their correlation with postoperative seizure reduction. RESULTS: After 3 months of stimulation, GM density reduced in right cerebellum, left superior temporal gyrus, right inferior temporal gyrus and left thalamus, and increased in left cerebellum, left inferior parietal lobule, left middle occipital gyrus and left gyrus rectus. No significant volume changes had been found in 14 subcortical nuclei. The fractional anisotropy (FA) values reduced in left superior longitudinal fasciculus and left corticospinal tract, and increased in bilateral cingulum and body of corpus callosum. The mean diffusivity (MD) values reduced in right retrolenticular part of internal capsule, right posterior corona radiata and right superior longitudinal fasciculus. The seizure reduction had positive correlation trends with the volume reduction in left nucleus accumbens and right amygdala, and MD reduction in right medial lemniscus and right posterior corona radiata. CONCLUSIONS: The results showed that VNS could cause changes of GM density, WM FA and MD values in epilepsy patients. The volume and MD reduction in some subcortical structures might participate in the seizure frequency reduction of VNS.

20.
J Cell Mol Med ; 25(20): 9724-9739, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34514712

RESUMO

Sepsis and sepsis-induced skeletal muscle atrophy are common in patients in intensive care units with high mortality, while the mechanisms are controversial and complicated. In the present study, the atrophy of skeletal muscle was evaluated in sepsis mouse model as well as the apoptosis of muscle fibres. Sepsis induced atrophy of skeletal muscle and apoptosis of myofibres in vivo and in vitro. In cell-based in vitro experiments, lipopolysaccharide (LPS) stimulation also inhibited the proliferation of myoblasts. At the molecular level, the expression of polo-like kinase 1 (PLK1) and phosphorylated protein kinase B (p-AKT) was decreased. Overexpression of PLK1 partly rescued LPS-induced apoptosis, proliferation suppression and atrophy in C2C12 cells. Furthermore, inhibiting the AKT pathway deteriorated LPS-induced atrophy in PLK1-overexpressing C2C12 myotubes. PLK1 was found to participate in regulating apoptosis and E3 ubiquitin ligase activity in C2C12 cells. Taken together, these results indicate that sepsis induces skeletal muscle atrophy by promoting apoptosis of muscle fibres and inhibiting proliferation of myoblasts via regulation of the PLK1-AKT pathway. These findings enhance understanding of the mechanism of sepsis-induced skeletal muscle atrophy.

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