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1.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 479-485, 2019 Oct 12.
Artigo em Chinês | MEDLINE | ID: mdl-31713375

RESUMO

OBJECTIVE: To understand the prevalence and risk factors of Blastocystis hominis infection in inpatients in Jiangjin District, Chongqing City. METHODS: A cross-sectional study was conducted in a community hospital in Jiangjin District, Chongqing City, and the inpatients were surveyed by questionnaires. After obtaining the informed consent from the inpatients or legal guardians, the stool and blood samples were collected and examined by microscopy and PCR from April 17 to May 1, 2018. The univariate analysis and logistic regression analysis were used to analyze the risk factors of the B. hominis infection. RESULTS: A total of 198 hospitalized patients were investigated, and the infection rate of B. hominis was 10.61% (21/198), and the infection rate of the females (12.10%) was higher than that of the males (8.11%), but the difference was not statistically significant. The highest rate of infection was 19.23% in the age group of 10 to 20 years, followed by 17.74% in the age group of 60 years and above, and the lowest rate was 2.38% in the age group of 20 to 40 years. The difference in infection rates of B. hominis among the different age groups was statistically significant (P < 0.05). The infection rate of B. hominis in the people who used dry pail latrines was 33.30%, which was higher than that of the people who used water flush toilets (9.10%) (P < 0.05). The genotypes of B. hominis were ST1, ST3, ST6 and ST7, and ST6 and ST3 being the most predominant genotypes which accounted for 47.62% (10/21) and 38.10% (8/21) respectively, and among the infected males, the genotypes were only ST3 and ST6. The multiple logistic regression analysis showed that among the factors affecting B. hominis infection, only keeping pets was a risk factor [OR = 3.798, 95% CI (1.245, 11.581), P < 0.05]. CONCLUSIONS: A high prevalence of B. hominis infection is found in the inpatients in Jiangjin District, Chongqing City, the predominant genotypes are ST6 and ST3, and keeping pets may be one of the main risk factors.


Assuntos
Infecções por Blastocystis , Blastocystis hominis , Adolescente , Adulto , Infecções por Blastocystis/sangue , Infecções por Blastocystis/epidemiologia , Blastocystis hominis/genética , Criança , China/epidemiologia , Estudos Transversais , Fezes/parasitologia , Feminino , Hospitais/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Inquéritos e Questionários , Adulto Jovem
2.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 498-503, 2019 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-31713378

RESUMO

OBJECTIVE: To investigate the prevalence and risk factors of Blastocystis hominis infections among patients with HIV/AIDS in Fuyang City, Anhui Province. METHODS: A cross-sectional study was conducted in Fuyang City, Anhui Province in 2016. The demographic and socioeconomic status, and the lifestyle and production style were collected using a questionnaire survey. B. hominis DNA was detected in subjects'stool samples using a PCR assay, and the CD4+ T lymphocyte count and HIV viral load were measured in the subjects' blood samples. The risk factors of B. hominis infections among patients with HIV/AIDS were identified using univariate and multivariate logistic regression analyses. RESULTS: A total of 398 HIV/AIDS patients were enrolled in this study, with a mean age of 49.3 years, a mean body weight of 55.9 kg and a mean height of 164.4 cm. The prevalence of B. hominis infection was 6.78% in the study subjects, and no gender- (χ2 = 1.589, P = 0.207), education level- (χ2 =0.508, P = 0.776), marital status- (χ2 = 0.419, P = 0.811) or occupation-specific prevalence (χ2 = 2.744, P = 0.615) was detected. Among the patients with HIV/AIDS, there were no significant differences in the age (t = 0.370, P = 0.712), height (t = 1.587, P =0.113), body weight (t = 0.516, P = 0.606), CD4+ T lymphocyte count (t = 1.187, P = 0.230) or HIV viral load (t = 0.193, P =0.496) between B. hominis-infected and uninfected individuals. Dinking non-tap water [OR = 6.554, 95% CI: (1.876 to 22.903)] and keeping dogs [OR = 5.895, 95% CI: (2.017 to 17.225)] were identified as risk factors for B. hominis infection in patients with HIV/AIDS. CONCLUSIONS: The prevalence of B. hominis infection is high in HIV/AIDS patients, and drinking non-tap water and keeping dogs are risk factors for B. hominis infection among HIV/AIDS patients.


Assuntos
Infecções por Blastocystis , Blastocystis hominis , Infecções por HIV , Animais , Infecções por Blastocystis/complicações , Infecções por Blastocystis/epidemiologia , Estudos Transversais , Cães , Fezes/parasitologia , Feminino , HIV , Infecções por HIV/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco
3.
Zhonghua Yu Fang Yi Xue Za Zhi ; 52(8): 855-861, 2018 Aug 06.
Artigo em Chinês | MEDLINE | ID: mdl-30107722

RESUMO

Decabromodiphenyl ethane (DBDPE) is a kind of new brominated flame retardants, which is widely used as a replace of decabromodiphenyl ether in electronic appliances, textiles and other goods. This review summarizes environmental levels and body burden of human beings of DBDPE in recent years. The data shows that the concentration of DBDPE in the environment and human tissues shows an upward trend. According to limited experiments about its toxicity, DBDPE shows similar toxicity to decabromodiphenyl ether. DBDPE can interfere thyroid hormones balance, and cause damage to liver, reproductive development, kidney, et al, which implies that DBDPE might be another new persistent organic pollutant. Further researches are needed.


Assuntos
Bromobenzenos/toxicidade , Poluentes Ambientais/análise , Poluentes Ambientais/toxicidade , Retardadores de Chama , Humanos
4.
Zhonghua Nei Ke Za Zhi ; 57(2): 140-142, 2018 Feb 01.
Artigo em Chinês | MEDLINE | ID: mdl-29397602
5.
Breast Cancer Res Treat ; 168(2): 337-348, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29188398

RESUMO

PURPOSE: To investigate the efficacy and safety of doublet versus single-agent chemotherapy (CT) plus trastuzumab (H) as first-line therapy for human epidermal growth factor 2 receptor (HER2)-positive metastatic breast cancer (MBC). METHODS: We searched for randomized clinical trials (RCTs) that evaluated the treatment effects of single-agent or doublet CT+H as first-line therapies for HER2-positive MBC. The main outcomes measured for this study included the overall response rate (ORR), progression-free survival (PFS), and overall survival (OS). A meta-analysis and trial sequential analysis (TSA) were performed, and the study quality was evaluated using the GRADE framework. The PROSPERO registry number of our analysis is CRD42016043766. RESULTS: The results from four RCTs including 1044 participants were pooled. Moderate-quality evidence indicated that compared with single-agent CT+H, doublet CT+H correlated better with prolonged PFS (hazard ratio [HR] 0.69, 95% confidence interval [CI] 0.63-0.75, P < 0.0001) and OS (HR = 0.90, 95% CI 0.88-0.92, P < 0.0001). However, moderate-quality evidence revealed no significant difference between the two regimens regarding the ORR (relative risk [RR] = 1.07, 95% CI 0.98-1.17, P = 0.157), which was confirmed by TSA, indicating that the cumulative Z-curve entered the futility area. Moderate-quality evidence indicated that treatment-related grade 3 or 4 toxicities of thrombocytopenia (RR = 4.08, P = 0.000), nausea/vomiting (RR = 4.26, P = 0.002), diarrhea (RR = 2.81, P = 0.002), and stomatitis (RR = 5.02, P = 0.003) were observed more frequently with doublet CT+H than with single-agent CT+H. CONCLUSIONS: Compared with single-agent CT, the combination of doublet CT with trastuzumab as first-line therapy for HER2-positive MBC is associated with longer PFS and OS, but more treatment-related grade 3 or 4 toxicities. Therefore, doublet CT appears to be an appropriate regimen for HER2-positive MBC with a good performance status.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Receptor ErbB-2/metabolismo , Trastuzumab/uso terapêutico , Neoplasias da Mama/patologia , Diarreia/induzido quimicamente , Diarreia/epidemiologia , Intervalo Livre de Doença , Feminino , Humanos , Incidência , Náusea/induzido quimicamente , Náusea/epidemiologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Estomatite/induzido quimicamente , Estomatite/epidemiologia , Trombocitopenia/induzido quimicamente , Trombocitopenia/epidemiologia , Vômito/induzido quimicamente , Vômito/epidemiologia
6.
Nano Lett ; 18(1): 535-539, 2018 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-29244507

RESUMO

Achieving efficient localization of white light at the nanoscale is a major challenge due to the diffraction limit, and nanoscale emitters generating light with a broadband spectrum require complicated engineering. Here we suggest a simple, yet highly efficient, nanoscale white-light source based on a hybrid Si/Au nanoparticle with ultrabroadband (1.3-3.4 eV) spectral characteristics. We incorporate this novel source into a scanning-probe microscope and observe broadband spectrum of photoluminescence that allows fast mapping of local optical response of advanced nanophotonic structures with submicron resolution, thus realizing ultrabroadband near-field nanospectroscopy.

7.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 52(12): 881-884, 2017 Dec 07.
Artigo em Chinês | MEDLINE | ID: mdl-29262443

RESUMO

Objective: To investigate the relationship between the recurrence of benign paroxysmal positional vertigo(BPPV) and the levels of bone mineral density(BMD) and estrogen in postmenopausal women. Methods: A total of 38 postmenopausal women with recurrent BPPV were recruited as study group, in the First Affiliated Hospital of Soochow University from December 2013 to June 2017. Meanwhile, 49 normal menopausal women were included as control. All patients were natural menopausal for over one year.The patients were diagnosed as BPPV based on results of Dix-Hallpike test and Roll-test, with at least two episodes of recurrent onset. In the subjects, BMD was measured by dual X-ray absorptiometry of lumbar vertebrae. Estrogen levels were obtained by testing serum estradiol (E2) levels in early morning fasting venous blood. In the present study, we compared the level of E2 and the value of BMD in two groups by SPSS 21.0. In the study group, patients with decreased BMD were divided into two groups: treatment and untreated group. The recurrence rate of BPPV was compared between the two groups within 12 months. Results: ①The averagel levels of E2 and BMD in the study group were (16.21±11.00)ng/L and -1.68±0.98) respectively, which were significantly lower than those in the control group (t value was 7.03 and 8.05 respectively, both P<0.05). The averagel levels of E2 and BMD incontrol group were(28.52±6.34)ng/L and -0.18±0.77 respectively. ②The number of patients with decreased BMD in the study group (30 cases) was more than that in control group (6 cases), and the difference was statistically significant (P<0.05). ③ The recurrence rate of BPPV in treatment group [17.6%(3/17)] was significantly lower than that of untreated group [61.5%(8/13)], and the difference was statistically significant (P<0.05). Conclusion: Recurrent BPPV in postmenopausal women usually accompany with low levels of estrogen and BMD. Active treatment is helpful for their recurrence of BPPV.


Assuntos
Vertigem Posicional Paroxística Benigna/sangue , Vertigem Posicional Paroxística Benigna/fisiopatologia , Densidade Óssea , Estrogênios/sangue , Pós-Menopausa/sangue , Pós-Menopausa/fisiologia , Absorciometria de Fóton , Estudos de Casos e Controles , Feminino , Humanos , Vértebras Lombares/fisiologia , Menopausa/sangue , Menopausa/fisiologia , Recidiva
8.
Hong Kong Med J ; 23(1): 35-40, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27909269

RESUMO

INTRODUCTION: Many studies of patients' perception of a medical chaperone have focused on female patients; that of male patients are less well studied. Moreover, previous studies were largely based on patient populations in English-speaking countries. Therefore, this study was conducted to investigate the perception and attitude of male and female Chinese patients to the presence of a chaperone during an intimate physical examination. METHODS: A cross-sectional guided questionnaire survey was conducted on a convenient sample of 150 patients at a public teaching hospital in Hong Kong. RESULTS: Over 90% of the participants considered the presence of a chaperone appropriate during intimate physical examination, and 84% felt that doctors, irrespective of gender, should always request the presence of a chaperone. The most commonly cited reasons included the availability of an objective account should any legal issue arise, protection against sexual harassment, and to provide psychological support. This contrasted with the experience of those who had previously undergone an intimate physical examination of whom only 72.6% of women and 35.7% of men had reportedly been chaperoned. Among female participants, 75.0% preferred to be chaperoned during an intimate physical examination by a male doctor, and 28.6% would still prefer to be chaperoned when being examined by a female doctor. Among male participants, over 50% indicated no specific preference but a substantial minority reported a preference for chaperoned examination (21.2% for male doctor and 25.8% for female doctor). CONCLUSIONS: Patients in Hong Kong have a high degree of acceptance and expectations about the role of a medical chaperone. Both female and male patients prefer such practice regardless of physician gender. Doctors are strongly encouraged to discuss the issue openly with their patients before they conduct any intimate physical examination.


Assuntos
Atitude Frente a Saúde , Acompanhantes Formais em Exames Físicos/estatística & dados numéricos , Preferência do Paciente/estatística & dados numéricos , Exame Físico , Relações Médico-Paciente , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos Transversais , Feminino , Hong Kong , Hospitais de Ensino , Humanos , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários , Adulto Jovem
9.
Genet Mol Res ; 15(4)2016 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-28002611

RESUMO

Cryopreservation has been proven significance as a technique for promising the long-term conservation of plant germplasms. This study aimed to establish a cryopreservation protocol for calli of Schisandra chinensis (Turcz.) Baill, and to explore the effects of different process parameters on callus viability. Effects of desiccation duration, cryoprotectants and cryopreservation methods, thawing temperature, and post-culture conditions on the viability of cryopreserved calli were assessed. Among different cryoprotectants and freezing procedures, the highest survival was recorded when the water content of callus after 30 min desiccation was 57.3%, were loaded into a cryoprotectant containing 10% ethylene glycol, 8% glucose, and 10% DMSO, and frozen slowly (-1°C/min). Rapid thawing at 40°C for 2 min demonstrated the best recovery of cryopreserved S. chinensis calli. Post-culturing in darkness for one week before transfer to light conditions (under 16 h photoperiod at 36 µmol·m-2·s-1) was beneficial to callus regeneration. Plants regenerated through somatic embryogenesis from cryopreserved calli remained ploidy stable after cryopreservation. The callus cryopreservation procedure established in this study is a promising tool for the conservation of S. chinensis resources.


Assuntos
Criopreservação/métodos , Schisandra/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Crioprotetores/farmacologia , Dessecação , Poliploidia , Regeneração , Schisandra/efeitos dos fármacos
10.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 30(18): 1435-1437;1443, 2016 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-29871111

RESUMO

Objective:To explore the role of video nystagmography in the diagnosis and treatment of multiple benign paroxysmal positional vertigo. Method: Eleven patients of 313 patients with benign paroxysmal positional vertigo had been diagnosed as multiple benign paroxysmal positional vertigo.They were post and horizontal semicircular canal ipsilaterally, bilateral post semicircular canal, superior and horizontal semicircular canal ipsilaterally, and bilateral superior semicircular canal benign paroxysmal positional vertigo.Result:The patients were performed Dix-Hallpike test and Roll test under VNG, combined with track record by video nystagmography to confirm the affected sites. Six cases were post and horizontal semicircular canal benign paroxysmal positional vertigo ipsilaterally. Three cases were bilateral posterior semicircular canal benign paroxysmal positional vertigo, one case of superior semicircular canal and horizontal semicircular canal benign paroxysmal positional vertigo ipsilaterally, and one case of bilateral superior semicircular canal benign paroxysmal positional vertigo. The symptom of patients got relief after repositioning sequentially. We repositioned the affected canal with strong nystagmus and vertigo at the first time. Conclusion:Multiple benign paroxysmal positional vertigo was rare and easily misdiagnosed because of complex nystagmus. We can confirm the affected canal and intensity by video nystagmography and get good prognosis after repositioning sequentially.

11.
Zhonghua Yi Xue Za Zhi ; 96(48): 3902-3905, 2016 Dec 27.
Artigo em Chinês | MEDLINE | ID: mdl-28057162

RESUMO

Objective: To study the prevalence of obstructive sleep apnea hypopnea syndrome (OSAHS) and its relationship with traffic accidents in the professional drivers. Methods: Questionnaires of OSAHS were sent to 950 professional drivers who had annual physical examination at the Central Hospital of Jiading District in Shanghai from October 2014 to September 2015. Those with moderate to severe snoring and/or Epworth Sleepiness Scale (ESS)≥9 performed the home sleep testing. All drivers were divided into OSAHS and non-OSAHS according to the survey and monitoring. The following parameters were compared such as driving ages, neck circumference, body mass index (BMI), average night sleep time, ESS, hypertension, diabetes, hypertrophy of tonsil and the incidence of traffic accidents. The risk factors of traffic accidents were analyzed by multivariate Logistic regression. Results: Totally 826 responses were eligible, including 578 (70.0%) with self-reported snoring. There was measurement failure involving 3 of 233 the home sleep testing due to sensor off, 823 subjects were included in the study. The prevalence of OSAHS was 13.5% (111/823). The mild, moderate and severe OSAHS were 47, 38 and 26 cases respectively. There were 712 drives without OSAHS. The neck circumference[(39.8±3.8) vs (39.0±3.0) cm]and BMI[(26.7±4.2) vs (24.4±3.8) kg/m2]were significantly higher in the drivers suffering from OSAHS (all P<0.05). The percentage of ESS≥ 9 (57.7% vs 12.6%), hypertension (27.9% vs 5.9%), diabetes (4.5% vs 1.1%), hypertrophy of tonsil (7.2% vs 2.3%) were higher in the drivers with OSAHS (all P<0.05). There were no significant difference in driving ages and average night sleep time between two groups (all P>0.05). The overall incidence of traffic accidents was 5.8% (48/823) in a year. The percentage was respectively 17.1% (19/111) in OSAHS and 4.1% (29/712) in non-OSAHS (P<0.001). Multiple logistic regression analysis showed that sleepiness (OR=30.578, 95%CI: 10.699-87.394; P<0.001), OSAHS (OR=14.062, 95%CI: 4.791-41.269; P<0.001) and vehicle years (OR=2.345, 95%CI: 1.183-4.646; P<0.05)were the risk factors, while the average night sleep time (OR=0.037, 95%CI: 0.014-0.098; P<0.001) was the protective factor. Conclusion: Professional drivers have higher prevalence of OSAHS, which contributes to the increased risk of traffic accidents.


Assuntos
Acidentes de Trânsito , Condução de Veículo , Apneia Obstrutiva do Sono , Índice de Massa Corporal , China , Humanos , Hipertensão , Polissonografia , Prevalência , Fatores de Risco , Sono , Fases do Sono , Ronco , Inquéritos e Questionários , Síndrome
12.
Genet Mol Res ; 14(3): 8909-18, 2015 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-26345822

RESUMO

This study aimed to analyze the association between the 405G/C and -2578C/A polymorphisms of the vascular endothelial growth factor (VEGF) gene and breast cancer risk by meta-analysis. A systematic computerized search of PubMed, Google Scholar, and Web of Science databases was performed to identify relevant publications. After rigorous searching and screening, 9 eligible case-control studies were included in this meta-analysis. The associations between the VEGF gene 405G/C and -2578C/A polymorphisms and breast cancer risk were estimated by pooled ORs and 95%CIs using fixed- or random-effect models. Meta-analysis results showed no significant association between the 405G/C polymorphism and breast cancer risk (CC vs GG: OR = 1.04, 95%CI = 0.92-1.17; CC vs GC: OR = 1.04, 95%CI = 0.93-1.17; dominant model: OR = 0.95, 95%CI = 0.85-1.06; recessive model: OR = 0.92, 95%CI = 0.70-1.20). The results also did not show significant association for the -2578C/A polymorphism: (AA vs CC: OR = 1.03, 95%CI = 0.91-1.15; AA vs GA: OR = 0.99, 95%CI = 0.89-1.10; dominant model: OR = 1.00, 95%CI = 0.90-1.10; recessive model: OR = 1.03, 95%CI = 0.94-1.13). Similar results were observed in the subgroup analyses on ethnicity, sample size, and Hardy-Weinberg equilibrium. These findings suggested a lack of association between the VEGF gene 405G/C and -2578C/A polymorphisms and breast cancer susceptibility.


Assuntos
Neoplasias da Mama/genética , Fator A de Crescimento do Endotélio Vascular/genética , Estudos de Casos e Controles , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Razão de Chances , Polimorfismo de Nucleotídeo Único , Fatores de Risco
13.
Genet Mol Res ; 14(4): 18945-57, 2015 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-26782544

RESUMO

It has been reported that interleukin-10 (IL-10) promoter genes (1082 A/G, 819 T/C, 592 A/C) are associated with nasopharyngeal carcinoma (NPC). However, the results remain controversial and ambiguous. To resolve inconsistencies in published data, we performed a meta-analysis to ascertain the association between IL-10 polymorphisms and NPC risk. Two case-control studies and two cohort studies were quantitatively analyzed to evaluate IL-10 promoter gene polymorphisms and NPC risk. Odds ratios (ORs) and their 95% confidence intervals (CIs) were calculated for each genetic model and allelic comparison. A random-effect model or a fixed-effect model was used to calculate the overall combined risk estimates. Overall, the variant genotypes (AA and AG) of the IL-10-1082 A/G polymorphism were associated with elevated risk of NPC compared with the GG homozygote (AG vs GG: OR = 1.77; 95%CI = 1.39-2.26; AG + GG vs AA: OR = 1.78; 95%CI = 1.42-2.22); no significant associations were observed in allelic contrast and the recessive model. Strong positive association was seen in the cohort studies but not in the case-control studies. No statistically significant association was detected between IL-10-819 T/C and IL-10-592 A/C polymorphisms and NPC. Additionally, publication bias was not found. Based on the current evidence, this meta-analysis suggests that IL-1082 A/G polymorphism may increase the risk of NPC, but IL-10-819 T/C and IL-10-592 A/C polymorphisms do not. Further multicenter studies that are better controlled are required to confirm these findings.


Assuntos
Interleucina-10/genética , Neoplasias Nasofaríngeas/genética , Carcinoma , Estudos de Casos e Controles , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Carcinoma Nasofaríngeo , Polimorfismo de Nucleotídeo Único , Regiões Promotoras Genéticas , Fatores de Risco
14.
Eur J Histochem ; 58(3): 2415, 2014 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-25308847

RESUMO

Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-ß1 is able to induced Ank gene expression and protect chondrocyte calcification. Intermittent cyclic mechanical tension (ICMT) could induce calcification of endplate chondrocytes by decrease the expression of Ank gene. In this study, we investigated the relation of intermittent cyclic mechanical unconfined compression (ICMC) and Ank gene expression. We found that ICMC decreased the Ank gene expression in the endplate chondrocytes, and there was an decreased in the  TGF-ß1 expression after ICMC stimulation. The Ank gene expression significantly increased when treated by transforming growth factor alpha 1 (TGF-ß1) in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our results implicate that ICMC-induced downregulation of Ank gene expression may be regulated by TGF-ß1 in endplate chondrocytes.


Assuntos
Condrócitos/citologia , Condrócitos/metabolismo , Força Compressiva , Animais , Calcificação Fisiológica , Técnicas de Cultura de Células , Proliferação de Células , Sobrevivência Celular , Células Cultivadas , Ensaio de Imunoadsorção Enzimática , Proteínas de Transporte de Fosfato/metabolismo , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Fator de Crescimento Transformador beta1/metabolismo
15.
Acta Anaesthesiol Scand ; 57(10): 1301-7, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24032397

RESUMO

BACKGROUND: Few studies look into cerebral blood flow (CBF) changes during emergence from general anaesthesia for craniotomy. The purpose of this study was to assess CBF changes during emergence from general anaesthesia for craniotomy, through monitoring blood oxygen saturation of jugular vein bulb (SjvO2 ) and transcranial Doppler (TCD). METHODS: We enrolled 30 patients undergoing selective craniotomy (group C) for supratentorial brain tumour resection and 30 patients undergoing selective abdominal surgery (group A). Mean velocity of middle cerebral artery (Vmca), mean arterial pressure (MAP), SjvO2 (only measured in group C), and arterial CO2 partial pressure were measured before anaesthesia, at tracheal extubation, and 30, 60, 90, 120 min after extubation. RESULTS: Vmca of the same side of tumour was significantly higher than contralateral Vmca before anaesthesia and at all times after extubation in group C. The ipsilateral Vmca increased significantly (95.7 ± 16.9 cm/s vs. 63.7 ± 6.7 cm/s, P < 0.01) at extubation in group C, then declined but still above baseline significantly in the first 2 h after extubation. While Vmca of the right side changed only slightly (63.6 ± 7.7 cm/s vs. 61.8 ± 8.1 cm/s, P < 0.01) but significantly at extubation in group A. SjvO2 increased significantly (81.4% ± 7.4% vs. 60.9% ± 3.7%, P < 0.01) at extubation in group C, and remained above baseline significantly for 2 h. There was no significant correlation between Vmca and MAP at any time. CONCLUSIONS: Cerebral hyperaemia occurs after supratentorial brain tumour resection surgery. The hyperaemia is more pronounced on the same side as the tumour.


Assuntos
Anestesia por Inalação/métodos , Circulação Cerebrovascular/efeitos dos fármacos , Craniotomia/métodos , Hiperemia/etiologia , Isoflurano/farmacologia , Neoplasias Supratentoriais/cirurgia , Adulto , Pressão Arterial/efeitos dos fármacos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Oxigênio/sangue , Estudos Prospectivos , Neoplasias Supratentoriais/fisiopatologia
16.
Opt Express ; 20(16): 18005-15, 2012 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-23038348

RESUMO

This paper demonstrates the optical nonlinearity in opto-mechanical ring resonators that consist of a bus waveguide and two ring resonators, which is induced by the optical gradient force and characterized by the Kerr-like coefficient. Each ring resonator has a free-hanging arc that is perpendicularly deformable by an optical gradient force and subsequently this deformation changes the effective refractive index (ERI) of the ring resonator. The change of the ERI induces optical nonlinearity into the system, which is described by an equivalent Kerr coefficient (Kerr-like coefficient). Based on the experimental results, the Kerr-like coefficient of the ring resonator system falls in the range from 7.64 × 10(-12) to 2.01 × 10(-10) m(2)W(-1), which is at least 6-order higher than the silicon's Kerr coefficient. The dramatically improved optical nonlinearity in the opto-mechanical ring resonators promises potential applications in low power optical signal processing, modulation and bio-sensing.

17.
Int J Immunopathol Pharmacol ; 25(4): 967-76, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23298487

RESUMO

Bone morphogenetic protein 2 (BMP-2) is a member of the TGF-beta superfamily of signaling molecules, and has been shown to function as a tumor suppressor involved in development and progression of many malignancies. BMP-2 has previously been reported to be closely correlated with lung cancer. But, the role and molecular mechanisms of BMP-2 in lung cancer have not yet been comprehensively explained. The present study aims to elucidate the role of BMP-2 in growth and invasion of human lung adenocarcinoma (LAC) in vitro and in vivo. Adenovirus vector-mediated BMP-2 small hairpin RNA (shBMP-2) was used to transfect into A549 LAC cells to determine the functional relevance of BMP-2 and tumor growth and invasion in vitro and in vivo, and further investigate the expression levels of BMP-2, vascular endothelial growth factor (VEGF), matrix metallopeptidase-9 (MMP-9), phosphatidylinositol 3-kinase p85alpha (PI3Kp85alpha) and phosphorylated AKT (p-AKT). As a result, LAC cell proliferation and invasion were significantly diminished by knockdown of BMP-2 indicated by MTT and Transwell assays, and cell apoptosis and cycle arrest were markedly induced indicated by flow cytometry. When BMP-2 expression was knocked down, the expression of PI3Kp85alpha, p-AKT, VEGF and MMP-9 was also down-regulated in LAC cells. In addition, the tumor volumes in LAC subcutaneous nude mouse model treated with shBMP-2 were significantly smaller than those in control and ad-GFP groups. Taken together, our findings indicate that knockdown of BMP-2 inhibits growth and invasion of LAC cells possibly via blockade of the PI3K/AKT signaling pathway, and BMP-2 may be a potential therapeutic target for lung cancer.


Assuntos
Proteína Morfogenética Óssea 2/antagonistas & inibidores , Neoplasias Pulmonares/terapia , RNA Interferente Pequeno/genética , Adenoviridae/genética , Proteína Morfogenética Óssea 2/genética , Proteína Morfogenética Óssea 2/fisiologia , Pontos de Checagem do Ciclo Celular , Linhagem Celular Tumoral , Proliferação de Células , Classe Ia de Fosfatidilinositol 3-Quinase/análise , Classe Ia de Fosfatidilinositol 3-Quinase/fisiologia , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/patologia , Metaloproteinase 9 da Matriz/análise , Invasividade Neoplásica , Proteínas Proto-Oncogênicas c-akt/fisiologia , Fator A de Crescimento do Endotélio Vascular/análise
18.
Environ Pollut ; 159(10): 2310-9, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21616570

RESUMO

The Positive Matrix Factorization (PMF) receptor model and the Observation Based Model (OBM) were combined to analyze volatile organic compound (VOC) data collected at a suburban site (WQS) in the PRD region. The purposes are to estimate the VOC source apportionment and investigate the contributions of these sources and species of these sources to the O(3) formation in PRD. Ten VOC sources were identified. We further applied the PMF-extracted concentrations of these 10 sources into the OBM and found "solvent usage 1", "diesel vehicular emissions" and "biomass/biofuel burning" contributed most to the O(3) formation at WQS. Among these three sources, higher Relative Incremental Reactivity (RIR)-weighted values of ethene, toluene and m/p-xylene indicated that they were mainly responsible for local O(3) formation in the region. Sensitivity analysis revealed that the sources of "diesel vehicular emissions", "biomass/biofuel burning" and "solvent usage 1" had low uncertainties whereas "gasoline evaporation" showed the highest uncertainty.


Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental , Ozônio/análise , Compostos Orgânicos Voláteis/análise , Poluentes Atmosféricos/química , Poluição do Ar/estatística & dados numéricos , China , Fogo , Ozônio/química , Processos Fotoquímicos , Rios , Emissões de Veículos/análise , Compostos Orgânicos Voláteis/química
19.
Opt Express ; 18(21): 21651-62, 2010 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-20941064

RESUMO

This paper reports a pure angular momentum generator using a ring resonator surrounded by a group of nano-rods. The evanescent waves of the circulating light in the ring are scattered by the nano-rods and generate a rotating electromagnetic field, which has only angular momentum but no linear momentum along the axis of rotation. The angular order is determined by the difference between the order of Whispering Gallery mode and the number of the rods, the rotating frequency is equal to the light frequency divided by the angular order. The maximum amplitude of the rotating electromagnetic fields can be 10 times higher than the amplitude of the input field when there are 36 rods (R(rod) = 120 nm, nr = 1.6). The pure angular momentum generator provides a new platform for trapping and rotation of small particles.


Assuntos
Nanopartículas/química , Nanotecnologia/métodos , Óptica e Fotônica , Materiais Biocompatíveis/química , Biofísica/métodos , Lasers , Luz , Modelos Teóricos , Física/métodos , Radiação , Espalhamento de Radiação
20.
Artigo em Inglês | MEDLINE | ID: mdl-20589548

RESUMO

The aim of this study was to estimate the dietary intake of arsenic (As) by children and adults from Jinhu area of China. A duplicate diet approach was used to collect diet samples in November and December 2007. The As content was measured by hydride generation atomic fluorescence spectrometry. The As intakes (median) for different group were: children: 0.6 microg kg(-1) bw day(-1) or 6.7 microg kg(-1) bw week(-1); adults: 0.5 microg kg (-1) bw day(-1) or 3.8 microg kg (-1) bw week(-1), none of which exceeded the provisional tolerable weekly intake (PTWI) of 15 microg kg (-1) bw week(-1) for As proposed by the World Health Organization (WHO). The median and maximum of the children group amounted to 44.7% and 125.3% of the PTWI; the values for adults were 25.3% and 118.7%, respectively. There were 3.3% of children whose weekly As intake exceeded the PTWI, and there were 6.7% of adults whose weekly As intake exceeded the PTWI. It is concluded that dietary As intake was safe for most people in Jinhu area, but the high dietary As intake for a minority of people in this area deserves attention.


Assuntos
Arsênico/administração & dosagem , Dieta , Poluentes Ambientais/administração & dosagem , Adulto , Algoritmos , Arsênico/toxicidade , Intoxicação por Arsênico/epidemiologia , Carcinógenos/administração & dosagem , Carcinógenos/toxicidade , Pré-Escolar , China/epidemiologia , Estudos Transversais , Dieta/efeitos adversos , Poluentes Ambientais/toxicidade , Feminino , Análise de Alimentos , Política de Saúde , Humanos , Limite de Detecção , Masculino , Espectrometria de Fluorescência/métodos , Espectrofotometria Atômica/métodos
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