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1.
J Exp Clin Cancer Res ; 39(1): 123, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32605589

RESUMO

BACKGROUND: 3-Hydroxy butyrate dehydrogenase 2 (BDH2) is a short-chain dehydrogenase/reductase family member that plays a key role in the development and pathogenesis of human cancers. However, the role of BDH2 in gastric cancer (GC) remains largely unclear. Our study aimed to ascertain the regulatory mechanisms of BDH2 in GC, which could be used to develop new therapeutic strategies. METHODS: Western blotting, immunohistochemistry, and RT-PCR were used to investigate the expression of BDH2 in GC specimens and cell lines. Its correlation with the clinicopathological characteristics and prognosis of GC patients was analysed. Functional assays, such as CCK-8 and TUNEL assays, transmission electron microscopy, and an in vivo tumour growth assay, were performed to examine the proliferation, apoptosis, and autophagy of GC cells. Related molecular mechanisms were clarified by luciferase reporter, coimmunoprecipitation, and ubiquitination assays. RESULTS: BDH2 was markedly downregulated in GC tissues and cells, and the low expression of BDH2 was associated with poor survival of GC patients. Functionally, BDH2 overexpression significantly induced apoptosis and autophagy in vitro and in vivo. Mechanistically, BDH2 promoted Keap1 interaction with Nrf2 to increase the ubiquitination level of Nrf2. Ubiquitination/degradation of Nrf2 inhibited the activity of ARE to increase accumulation of reactive oxygen species (ROS), thereby inhibiting the phosphorylation levels of AktSer473 and mTORSer2448. CONCLUSIONS: Our study indicates that BDH2 is an important tumour suppressor in GC. BDH2 regulates intracellular ROS levels to mediate the PI3K/Akt/mTOR pathway through Keap1/Nrf2/ARE signalling, thereby inhibiting the growth of GC.

2.
Huan Jing Ke Xue ; 41(3): 1078-1084, 2020 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608607

RESUMO

To study the emission characteristics of volatile organic compounds (VOCs) in Zibo, nine key industries and their representative enterprises were selected to conduct a field investigation and measurement. The emitted VOC characteristics in different industries were analyzed. Based on measurement data, the emissions of VOCs from all monitored enterprises were calculated to obtain the localization emission factors. The results showed that different industries exhibited some differences in VOCs components, and the major VOCs components were alkane and halo hydrocarbon. Ethane, acetylene, chloromethane (conclude 1,1-dichloroethane, 1,1,1-trichloroethane), and Freon (Freon 12 or Freon 114) were the characteristic species in most industries. The results indicated that the major VOCs emission links in different types of petrochemical industries were equipment leakage, loading volatilization, storage volatilization losses, and organized discharge, which accounted for more than 40% of emissions. Local emission factors of VOCs calculated in the synthetic rubber and steel manufacturing industries were close to the recommended values in the guide, whereas there were large gaps in other industries.

3.
Int J Chron Obstruct Pulmon Dis ; 15: 1287-1299, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606639

RESUMO

Background: T helper (Th) cell cytokine imbalances have been associated with the pathophysiology of chronic obstructive pulmonary disease (COPD), including the Th1/Th2 and Th17/T regulatory cells (Treg) paradigms. Clarifying cytokine profiles during COPD acute exacerbation (AE) and their relationships with clinical manifestations would help in understanding the pathogenesis of disease and improve clinical management. Materials and Methods: Eighty seven patients admitted to the hospital with AEs of COPD were included in this study, and follow-up was conducted after discharge (every 30 days, for a total of 120 days). Sputum samples of patients at different time points (including at admission, discharge, and follow-up) were collected, and sputum cytokine profiling (12 cytokines in total) was performed using a Luminex assay. Results: According to the cytokine profiles at admission, patients were divided into three clusters by a k-means clustering algorithm, namely, Th1high Th17high (n=26), Th1lowTh17low (n=56), and Th1high Th17low (n=5), which revealed distinct clinical characteristics. Patients with Th1high Th17low profile had a significantly longer length of non-invasive ventilation time and length of hospital stay than patients with Th1high Th17high profile (7 vs 0 days, 22 vs 11 days, respectively, p < 0.05), and had the highest AE frequency. Sputum levels of Th17 cytokines (IL-17A, IL-22, and IL-23) during AE were negatively correlated with AE frequency in the last 12 months (r = -0.258, -0.289 and -0.216, respectively, p < 0.05). Moreover, decreased sputum IL-17A levels were independently associated with increased AE frequency, with an OR (95% CI) of 0.975 (0.958-0.993) and p = 0.006. Conclusion: Th1/Th17 imbalance during AE is associated with the severity of COPD. Decreased Th17 cytokine expression is correlated with increased AE frequency. The Th1/Th17 balance may be a specific target for the therapeutic manipulation of COPD.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32619356

RESUMO

This study aimed to investigate the effect of miniscrews thread shape on the stress distribution receiving a torque load. Seven thread shapes (S,V1,V2,B1,B2,R1,R2) models were constructed and a 6 Nmm-torque load was applied. The order of maximum equivalent stress (EQV) value was V1 > V2 > B1 > R1 > R2 > B2 > S. The order of maximum displacement of miniscrew (Max DM) value was S > B2 > R1 = V1 > B1 > V2 > R2. Model R2 may be the most appropriate thread shape affording a torque force.

5.
Biomed Res Int ; 2020: 8542797, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32626767

RESUMO

The section Pallasia is one of the components of the genus Allium subgenus Allium (Amaryllidaceae), and species relationship in this section is still not resolved very well, which hinders further evolutionary and adaptive studies. Here, the complete chloroplast genomes of five sect. Pallasia species were reported, and a comparative analysis was performed with other three related Allium species. The genome size of the eight species ranged from 151,672 bp to 153,339 bp in length, GC content changed from 36.7% to 36.8%, and 130 genes (except Allium pallasii), 37 tRNA, and 8 rRNA were identified in each genome. By analyzing the IR/LSC and IR/SSC boundary, A. pallasii exhibited differences compared with other seven species. Phylogenetic analysis achieved high supports in each branch, seven of the eight Allium species cluster into a group, and A. pallasii exhibit a close relationship with A. obliquum. Higher pairwise Ka/Ks ratios were found in A. schoenoprasoides compared to A. caeruleum and A. macrostemon while a lower value of Ka/Ks ratios was detected between A. caeruleum and A. macrostemon. This study will be a great contribution to the future phylogenetic and adaptive research in Allium.

6.
Pharmacol Res ; 159: 104926, 2020 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-32502636

RESUMO

Cardiac remodeling is accompanied by cardiac hypertrophy, fibrosis, dysfunction, and eventually leading to heart failure. Intermedin (IMD), as a paracrine/autocrine peptide, has a protective effect in cardiovascular diseases. In this study, we elucidated the role and the underlying mechanism of IMD in pathological remodeling. Pathological remodeling mouse models were induced by abdominal aorta constriction for 4 weeks or angiotensin II (Ang II) infusion for 2 weeks in wildtype, IMD-overexpression, IMD-knockout and klotho-knockdown mice. Western blot, real-time PCR, histological staining, echocardiography and hemodynamics were used to detect the role of IMD in cardiac remodeling. Cardiac hypertrophy, fibrosis and dysfunction were significantly aggravated in IMD-knockout mice versus wildtype mice, and the expression of klotho was downregulated. Conversely, cardiac remodeling was alleviated in IMD-overexpression mice, and the expression of klotho was upregulated. Hypertension induced by Ang II infusion rather than abdominal aorta constriction was mitigated by IMD. However, the cardioprotective effect of IMD was blocked in klotho-knockdown mice. Similar results were found in cultured neonatal rat cardiomyocytes, which was pretreated with IMD before Ang II stimulation. Mechanistically, IMD inhibited the phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) and the activity of calcineurin to protect against cardiac hypertrophy through upregulating klotho in vivo and in vitro. Furthermore, peroxisome proliferator-activated receptor γ (PPARγ) might mediate IMD upregulating klotho. In conclusion, pathological remodeling may be alleviated by endogenous IMD, which inhibits the expression of calcineurin and p-CaMKII by upregulating klotho via the PPARγ pathway. It suggested that IMD might be a therapeutic target for heart disease.

7.
IEEE Trans Cybern ; PP2020 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-32568717

RESUMO

Automatic image captioning is to conduct the cross-modal conversion from image visual content to natural language text. Involving computer vision (CV) and natural language processing (NLP), it has become one of the most sophisticated research issues in the artificial-intelligence area. Based on the deep neural network, the neural image caption (NIC) model has achieved remarkable performance in image captioning, yet there still remain some essential challenges, such as the deviation between descriptive sentences generated by the model and the intrinsic content expressed by the image, the low accuracy of the image scene description, and the monotony of generated sentences. In addition, most of the current datasets and methods for image captioning are in English. However, considering the distinction between Chinese and English in syntax and semantics, it is necessary to develop specialized Chinese image caption generation methods to accommodate the difference. To solve the aforementioned problems, we design the NICVATP2L model via visual attention and topic modeling, in which the visual attention mechanism reduces the deviation and the topic model improves the accuracy and diversity of generated sentences. Specifically, in the encoding phase, convolutional neural network (CNN) and topic model are used to extract visual and topic features of the input images, respectively. In the decoding phase, an attention mechanism is applied to processing image visual features for obtaining image visual region features. Finally, the topic features and the visual region features are combined to guide the two-layer long short-term memory (LSTM) network for generating Chinese image captions. To justify our model, we have conducted experiments over the Chinese AIC-ICC image dataset. The experimental results show that our model can automatically generate more informative and descriptive captions in Chinese in a more natural way, and it outperforms the existing image captioning NIC model.

8.
Anticancer Res ; 40(6): 3255-3264, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32487620

RESUMO

BACKGROUND/AIM: Rta, a transactivator of Epstein-Barr virus, is associated with progression of nasopharyngel carcinoma (NPC); however, its mechanism of contribution to the pathogenesis of NPC remains unclear. Interleukin-6 (IL-6), a tumor promoter, is detected in NPC. This in vitro study examined whether and how Rta promotes NPC progression by up-regulating IL-6. MATERIALS AND METHODS: Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time PCR, ELISA, immunoblotting assays, reporter gene assays, and transwell migration assays were performed. RESULTS: In NPC cells, Rta up-regulated IL-6 expression at the mRNA and protein levels, and the Rta's C-terminus was essential for promoter activation and expression of IL-6. The induction of IL-6 by Rta also required activation of extracellular signal-regulated kinase 1/2 and activator protein-1. Furthermore, IL-6 secreted from Rta-expressing NPC cells promoted migration of Rta-negative NPC cells by activating IL-6 receptor/Janus kinase/signal transducer and activator of transcription 3 pathway. CONCLUSION: Rta contributes to progression of NPC cells through induction of IL-6 in vitro.

9.
Clin Epigenetics ; 12(1): 80, 2020 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-32503656

RESUMO

BACKGROUND: The chromatin insulator CCCTC-binding factor (CTCF) displays tissue-specific DNA binding sites that regulate transcription and chromatin organization. Despite evidence linking CTCF to the protection of epigenetic states through barrier insulation, the impact of CTCF loss on genome-wide DNA methylation sites in human cancer remains undefined. RESULTS: Here, we demonstrate that prostate and breast cancers within The Cancer Genome Atlas (TCGA) exhibit frequent copy number loss of CTCF and that this loss is associated with increased DNA methylation events that occur preferentially at CTCF binding sites. CTCF sites differ among tumor types and result in tissue-specific methylation patterns with little overlap between breast and prostate cancers. DNA methylation and transcriptome profiling in vitro establish that forced downregulation of CTCF leads to spatially distinct DNA hypermethylation surrounding CTCF binding sites, loss of CTCF binding, and decreased gene expression that is also seen in human tumors. DNA methylation inhibition reverses loss of expression at these CTCF-regulated genes. CONCLUSION: These findings establish CTCF loss as a major mediator in directing localized DNA hypermethylation events in a tissue-specific fashion and further support its role as a driver of the cancer phenotype.

10.
Nat Commun ; 11(1): 2893, 2020 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-32518232

RESUMO

Africa contains some of the most vulnerable ecosystems to fires. Successful seasonal prediction of fire activity over these fire-prone regions remains a challenge and relies heavily on in-depth understanding of various driving mechanisms underlying fire evolution. Here, we assess the seasonal environmental drivers and predictability of African fire using the analytical framework of Stepwise Generalized Equilibrium Feedback Assessment (SGEFA) and machine learning techniques (MLTs). The impacts of sea-surface temperature, soil moisture, and leaf area index are quantified and found to dominate the fire seasonal variability by regulating regional burning condition and fuel supply. Compared with previously-identified atmospheric and socioeconomic predictors, these slowly evolving oceanic and terrestrial predictors are further identified to determine the seasonal predictability of fire activity in Africa. Our combined SGEFA-MLT approach achieves skillful prediction of African fire one month in advance and can be generalized to provide seasonal estimates of regional and global fire risk.

11.
Biomed Res Int ; 2020: 4675395, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32596314

RESUMO

All drugs usually have side effects, which endanger the health of patients. To identify potential side effects of drugs, biological and pharmacological experiments are done but are expensive and time-consuming. So, computation-based methods have been developed to accurately and quickly predict side effects. To predict potential associations between drugs and side effects, we propose a novel method called the Triple Matrix Factorization- (TMF-) based model. TMF is built by the biprojection matrix and latent feature of kernels, which is based on Low Rank Approximation (LRA). LRA could construct a lower rank matrix to approximate the original matrix, which not only retains the characteristics of the original matrix but also reduces the storage space and computational complexity of the data. To fuse multivariate information, multiple kernel matrices are constructed and integrated via Kernel Target Alignment-based Multiple Kernel Learning (KTA-MKL) in drug and side effect space, respectively. Compared with other methods, our model achieves better performance on three benchmark datasets. The values of the Area Under the Precision-Recall curve (AUPR) are 0.677, 0.685, and 0.680 on three datasets, respectively.

12.
J Cell Mol Med ; 2020 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-32510753

RESUMO

Methyl-CpG-binding protein 2 (MeCP2) is an important epigenetic regulator for normal neuronal maturation and brain glial cell function. Additionally, MeCP2 is also involved in a variety of cancers, such as breast, prostate, lung, liver and colorectal. However, whether MeCP2 contributes to the progression of breast cancer remains unknown. In the present study, we investigated the role of MeCP2 in cell proliferation, migration and invasion in vitro. We found that knockdown of MeCP2 inhibited expression of epithelial-mesenchymal transition (EMT)-related markers in breast cancer cell lines. In conclusion, our study suggests that MeCP2 inhibits proliferation and invasion through suppression of the EMT pathway in breast cancer.

13.
Gastric Cancer ; 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32529327

RESUMO

BACKGROUND: Gastric cancer (GC) is a leading cause of cancer-related mortality worldwide, because of the low efficacy of current therapeutic strategies. Estrogen-related receptor γ (ERRγ) was previously showed as a suppressor of GC. However, the mechanism and effective therapeutic method based on ERRγ is yet to be developed. METHODS: The expression levels of ERRγ, EZH2, and FOXM1 were detected by immunohistochemistry, qRT-PCR, and western blot. The regulatory mechanisms of ERRγ and FOXM1 were analyzed by ChIP, EMSA, and siRNA. The effects of EZH2 inhibitor (GSK126) or/and ERRγ agonist (DY131) on the tumorigenesis of gastric cancer cell lines were examined by cell proliferation, transwell migration, wound healing, and colony formation assays. Meanwhile, the inhibitory effects of GSK126 or/and DY131 on tumor growth were analyzed by xenograft tumor growth assay. RESULTS: The expression of ERRγ was suppressed in tumor tissues of GC patients and positively correlated with prognosis, as opposed to that of EZH2 and FOXM1. EZH2 transcriptionally suppressed ERRγ via H3K27me3, which subsequently activated the expression of master oncogene FOXM1. The combination of GSK126 and DY131 synergistically activated ERRγ expression, which subsequently inhibited the expression of FOXM1 and its regulated pathways. Synergistic combination of GSK126 and DY131 significantly inhibited the tumorigenesis of GC cell lines and suppressed the growth of GC xenograft. CONCLUSION: The FOXM1 signaling pathway underlying the ERRγ-mediated gastric cancer suppression was identified. Furthermore, combined treatment with EZH2 inhibitor and ERRγ agonist synergistically suppressed GC progression by inhibiting this signaling pathway, suggesting its high potential in treating GC patients.

14.
PLoS One ; 15(6): e0234555, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32525905

RESUMO

OBJECTIVES: Larval indices have been used for Ae. albopictus surveillance for many years, while there is limited use in assessing dengue transmission risk and adult mosquito emergence. This study is aimed to explore the relationships between larval indices and the Ae. albopictus density captured by BG-mosquito trap (BG-trap) method, with considering the meteorological factors. METHODS: Data on larval density, adult mosquito density and meteorology factors were collected in an entomological survey carried out in Quzhou City, Zhejiang Province of China in 2018. The Spearman's rank correlation and Pearson correlation were used for the analysis on the correlation of density indices. Generalized additive models were established to analyze the influencing factors of mosquito density. RESULTS: Breteau index (BI), House index (HI) and Container index (CI) were highly correlated with each other (r>0.7, p<0.05). The Ae. albopictus density was significantly correlated with CI (rs = 0.260, p<0.05), CI pre one week (rs = 0.259, p<0.05), and CI pre three weeks (rs = 0.329, p<0.05). BI was correlated with female Ae. albopictus density pre 4 weeks (r = -0.299, p<0.05). Female Ae. albopictus density was correlated with CI pre 3 weeks (rs = 0.303, p<0.05). The influencing factors of BI were average wind speed pre 1 week, average temperature and female Ae. albopictus density pre 4 weeks. The influencing factors of CI were average humidity pre 3 weeks and average temperature. The influencing factors of HI were average temperature and precipitation pre 4 weeks. The influencing factor of Ae. albopictus density and female Ae. albopictus density was temperature. CONCLUSIONS: The adult Ae. albopictus density had low correlation with certain larval indices. Some of the meteorology factors played significant roles in the density of adult Ae. albopictus and larva with or without a time lag.

15.
Small ; : e2001989, 2020 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-32521092

RESUMO

Fluorinated solid-electrolyte interphase (SEI) derived from fluoroethylene carbonate (FEC) is particularly favored for dendrite suppression in lithium metal batteries because of the high Young's modulus (≈64.9 Gpa) and low electronic conductivity (10-31 S cm-1 ) of LiF. However, the transportation ability of Li+ in this fluorinated SEI under high current densities is limited by the low ionic conductivity of LiF (≈10-12 S cm-1 ). Herein, by rational design, 0.1 m lithium bisoxalatodifluorophosphate (LiDFBOP) is adopted to modify fluorinated SEI in FEC based electrolyte for fast charging lithium metal batteries. Benefiting from the synergetic effect of LiDFBOP and FEC, a fluorinated SEI rich in LiF and Lix POy Fz species can be yielded, which can further improve the stability and ionic conductivity of SEI for fast Li+ transportation. Meanwhile, the average coulombic efficiency for Li plating/stripping is improved from 92.0% to 96.7%, thus promoting stable cycling of Li||Li symmetrical batteries with dendrite free morphologies, even at high current densities (3.0 mA cm-2 ) and high plating/stripping capacities (3.0 mAh cm-2 ). More attractively, in practical Li||LiNi0.6 Co0.2 Mn0.2 O2 batteries, the cycling life at 1C and rate capacities at 6C are also significantly improved. Therefore, the synergetic effect of LiDFBOP and FEC provides great potential for achieving advanced lithium metal batteries with fast charging ability.

16.
J Neurol ; 2020 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-32524258

RESUMO

OBJECTIVE: Dyspnea can be present as non-motor symptom in patients with Parkinson's disease (PD). Deep brain stimulation (DBS) of the subthalamic nucleus (STN) improves motor and non-motor symptoms in PD. However, new-onset dyspnea has been reported after DBS surgery. We have studied respiratory characteristics of PD patients with bilateral STN-DBS to assess the impact of DBS on pulmonary function. METHODS: STN-DBS PD patients with dyspnea after surgery (cases) were matched with STN-DBS PD patients without dyspnea (controls). Motor and pulmonary function were assessed with stimulation and without medication (on stim/off med), and without stimulation and medication (off stim/off med). Pulmonary function was investigated with spirometry and dyspnea with the Medical Research Council Dyspnea Scale (MRCDS) and the Borg Scale (BS). RESULTS: Seven cases (five men, 58.30 ± 6.70 years of age) and seven controls (six men, 61.10 ± 6.30 years of age) were enrolled. MRCDS and BS revealed the presence of dyspnea in both groups. No significant changes in pulmonary function were found in both cases and controls in on stim/off med vs. off stim/off med condition (p < 0.05), and in cases vs. controls in on stim/ off med condition (p < 0.05). CONCLUSIONS: No impact of STN-DBS on pulmonary function was found in cases. Impaired perception of dyspnea and spread of stimulation surrounding the STN might account for new-onset dyspnea after DBS surgery. Dyspnea was detected also in controls using ad hoc questionnaires. Our findings suggest further investigation of this non-motor symptom in PD patients.

17.
ACS Nano ; 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32530269

RESUMO

Alkali metals are ideal anodes for high-energy-density rechargeable batteries, while seriously hampered by limited cycle life and low areal capacities. To this end, rationally designed frameworks for dendrite-free and volume-changeless alkali-metal deposition at both high current densities and capacities are urgently required. Herein, a general 3D conductive Ti3C2TX MXene-melamine foam (MXene-MF) is demonstrated as an elastic scaffold for dendrite-free, high-areal-capacity alkali anodes (Li, Na, K). Owing to the lithiophilic nature of F-terminated MXene, conductive macroporous network, and excellent mechanical toughness, the constructed MXene-MF synchronously achieves a high current density of 50 mA cm-2 for Li plating, high areal capacity (50 mAh cm-2) with high Coulombic efficiency (99%), and long lifetime (3800 h), surpassing the Li anodes reported recently. Meanwhile, MXene-MF shows flat voltage profiles for 720 h at 10 mA cm-2 for the Na anode and 800 h at 5 mA cm-2 for the K anode, indicative of the wide applicability. Notably, the high current density of 20 mA cm-2 for 20 mAh cm-2 for the Na anode, accompanying good recyclability was rarely achieved before. When coupled with sulfur or Na3V2(PO4)3 cathodes, the assembled MXene-MF alkali (Li, Na)-based full batteries showcase enhanced rate capability and cycling stability, demonstrating the potential of MXene-MF for advanced alkali-metal batteries.

18.
Biomed Pharmacother ; 127: 110232, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32559854

RESUMO

Interleukin (IL)-33 is a member of the IL-1 family, participating in both helper T1 (Th1)- and Th2-type immune responses, but its ambiguous effects on tumor growth and related immune mechanisms remain unclear. Here, we report that recombinant mouse IL-33 (mIL-33) significantly inhibited colon cancer growth and metastasis to lung and liver in a murine CT26 or MC38 tumor-cell engraftment model. This effect could be associated with CD4+ T cells and CD40 L signaling, as depletion of CD4+ T cells or blocking CD40 L signaling in vivo partly abolished the antitumor function of IL-33. In addition, IL-33 treatment upregulated CD40 L expression on tumor-infiltrating lymphocytes, and promoted the activation of CD4+ T, CD8+ T and natural killer cells via CD40 L signaling. Furthermore, IL-33 was sufficient to induce the ST2 expression on CD4+ T cells, but not on CD8+ T and natural killer cells, indicating that IL-33 acted on CD4+ T cells via a positive-feedback loop. Our findings shed new light on the IL-33-mediated antitumor effects and mechanisms of Th1 action, and also suggest that IL-33 may serve as an activator to boost anticancer immune responses in singular or combinatory therapies.

19.
Open Heart ; 7(1)2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32393654

RESUMO

OBJECTIVE: To test whether intensive atorvastatin (ATV) increases the efficacy of transplantation with autologous bone marrow mononuclear cells (MNCs) in patients suffering from anterior ST-elevated myocardial infarction (STEMI). METHODS: This clinical trial was under a 2×2 factorial design, enrolling 100 STEMI patients, randomly into four groups of regular (RA) or intensive ATV (IA) with MNCs or placebo. The primary endpoint was the change of left ventricular ejection fraction (LVEF) at 1-year follow-up from baseline, primarily assessed by MRI. The secondary endpoints included other parameters of cardiac function, remodelling and regeneration determined by MRI, echocardiography, positron emission tomography (PET) and biomarkers. RESULTS: All the STEMI patients with transplantation of MNCs showed significantly increased LVEF change values than those with placebo (p=0.01) with only in the IA+MNCs patients group demonstrating significantly elevation of LVEF than in the IA+placebo group (+12.6% (95%CI 10.4 to 19.3) vs +5.0% (95%CI 4.0 to 10.0), p=0.001), pointing to a better synergy between ATV and MNCs (p=0.019). PET analysis revealed significantly increased viable areas of myocardium (p=0.015), while the scar sizes (p=0.026) and blood aminoterminal pro-B-type natriuretic peptide (p<0.034) reduced. All these above benefits of MNCs were also attributed to IA+MNCs instead of RA+MNCs group of patients with STEMI. CONCLUSIONS: Intensive ATV treatment augments the therapeutic efficacy of MNCs in patients with anterior STEMI at the convalescent stage. The treatment with the protocol of intensive ATV and MNC combination offers a clinically essential approach for myocardial infarction. TRIAL REGISTRATION NUMBER: NCT00979758.

20.
Can Fam Physician ; 66(5): 377, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32404460
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