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1.
World Neurosurg ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32058118

RESUMO

Angioleiomyoma is a kind of benign soft tissue tumor that manifests as pain and is more common in the extremities. However, primary intracranial angioleiomyoma is an extremely rare entity with poor clinical, radiological, and histopathological characterization. We will compile and examine reported cases of intracranial angioleiomyoma in an attempt to provide an up-to-date summary of the condition. A literature search was performed using PubMed with specific key terms. Selected case studies and case series were then compared, and statistical analyses were performed where appropriate. We report a 59-year-old woman presenting with epileptic seizures and a 2-month history of progressive headache. Magnetic resonance imaging(MRI) of the brain revealed a right temporal pole tumor, near the right cavernous sinus. Gross total resection was performed. Histopathological and immunohistochemical examination revealed an angioleiomyoma. No adjuvant radiation or chemotherapy was administered. MRI of the brain performed at a 6-month follow-up showed no signs of recurrence. Primary intracranial angioleiomyoma is an exceedingly rare central nervous system tumor. The clinical and radiological manifestations are nonspecific. The diagnosis depends on the histopathological and immunohistochemical examination. For patients with clinical symptoms,surgical resection should be the first-choice treatment.

2.
Inorg Chem ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32017542

RESUMO

The directing effect of coordinating ligands in the formation of uranium molecular complexes has been well established, but the role of counterions in metal-ligand interactions remains ambiguous and requires further investigation. In this work, we describe the targeted isolation, through the choice of alkali-metal ions, of a family of tetravalent uranium sulfates, showing the influence of the overall topology and, unexpectedly, the UIV nuclearity upon the inclusion of such countercations. Analyses of the structures of uranium(IV) oxo/hydroxosulfate oligomeric species isolated from consistent synthetic conditions reveal that the incorporation of Na+ and Rb+ promotes the crystallization of 0D discrete clusters with a hexanuclear [U6O4(OH)4(H2O)4]12+ core, whereas the larger Cs+ ion allows for the isolation of a 2D-layered oligomer with a less condensed trinuclear [U3(O)]10+ center. This finding expands the prevalent view that counterions play an innocent role in molecular complex synthesis, affecting only the overall packing but not the local oligomerization. Interestingly, trends in nuclearity appear to correlate with the hydration enthalpies of alkali-metal cations, such that the alkali-metal cations with larger hydration enthalpies correspond to more hydrated complexes and cluster cores. These findings afford new insights into the mechanism of nucleation of UIV, and they also open a new path for the rational design and synthesis of targeted molecular complexes.

3.
Eur J Med Chem ; 190: 112105, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32035399

RESUMO

A novel series of shikonin-benzo[b]furan derivatives were designed and synthesized as tubulin polymerization inhibitors, and their biological activities were evaluated. Most compounds revealed the comparable anti-proliferation activities against the cancer cell lines to that of shikonin and simultaneously low cytotoxicity to non-cancer cells. Among them, compound 6c displayed powerful anti-cancer activity with the IC50 value of 0.18 µM against HT29 cells, which was significantly better than that of the reference drugs shikonin and CA-4. What's more, 6c could inhibit tubulin polymerization and compete with [3H] colchicine in binding to tubulin. Further biological studies depicted that 6c can induce cell apoptosis and cell mitochondria depolarize, regulate the expression of apoptosis related proteins in HT29 cells. Besides, 6c actuated the HT29 cell cycle arrest at G2/M phase, and influenced the expression of the cell-cycle related protein. Moreover, 6c displayed potent inhibition on cell migration and tube formation that contributes to the antiangiogenesis. These results prompt us to consider 6c as a potential tubulin polymerization inhibitor and is worthy for further study.

4.
Colloids Surf B Biointerfaces ; 189: 110844, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32044675

RESUMO

Drug distribution in polymer dissolvable microneedles (MNs) is essential for enhancing the efficiency of drug delivery. In the present work, multiscale simulation was applied to study the interactions between polymer and drug molecules, which may influence the drug distribution in the MNs. In this study, Hyaluronic acid (HA) and Polyvinyl alcohol (PVA) were used to fabricate the MNs and sulfonhodamine B (SRB) was selected as the model drug. Firstly, from the quantum chemical calculations, the global electronegativity of HA (3.786 eV) is stronger than that of PVA (2.435 eV), which means that HA owns stronger electronegativity. The Flory-Huggins parameter of HA-SRB is -1.16 which is lower than that of PVA-SRB (53.51), indicating that HA has better compatibility with SRB molecules than PVA. From molecular dynamic simulations, the binding energy of HA-SRB is 93.52 kcal/mol which is much higher than that of PVA-SRB (-2.40 kcal/mol), meaning that HA is easier than PVA to combined with SRB. The mesoscale-based dissipative particle dynamics (DPD) simulations were applied to visualize the diffusion behavior of SRB and the swelling properties of the polymers. All the results indicated that SRB has a lower diffusion coefficient in PVA solution than that in HA solution, which may prevent the diffusion of drug from MN tips to the bases, facilitating the fabrication of MNs with drug concentrated MN tips. Finally, the SRB loaded PVA and HA MNs were prepared and the experimental results are consisted with the simulation results.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32031366

RESUMO

Coordination compound derivates (CCDs) have been widely applied in lithium-ion batteries (LIBs). However, the functionalization of CCDs with open structure and heteroatomic doping is still challenging. Herein, an effective functionalization strategy is presented to prepare a kind of CCD, where Co0.85Se nanoparticles are encapsulated by nitrogen enriched hierarchically porous carbon (CS/NPC) based on the heat treatment of a cobalt-complexed hexamethylolmelamine-polyvinylpyrrolidone resin (Co2+/HPR). The abundant free volume and enriched N species of the HPR are responsible for the hierarchically porous configuration and high N-doping of the composites. By controlling the content of cobalt salt and the heating rate, high specific surface area (440.7 m2 g-1), high N-doping (8.54 at%) and suitable mass loading of Co0.85Se (55.5 %) were simultaneously achieved in CS-2/NPC-5, which delivers a high reversible capacity (758.5 mAh g-1 at 0.1 A g-1), excellent rate capability (401.7 mAh g-1 at 5 A g-1), and stable cycling performance (638.4 mAh g-1 at 1 A g-1 after 200 cycles), demonstrating its great potential for LIB applications.

6.
Medicine (Baltimore) ; 99(7): e19142, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32049837

RESUMO

BACKGROUND: Depression is a kind of chronic and recurrent mental disorder, the main clinical characteristics of the patients are marked and persistent depression. At the same time, it is often accompanied by chronic physical disease, cognitive impairment, and functional damage, which is one of the common diseases that seriously threaten human health. At present, 3 kinds of oral Chinese patent medicine have clinical comparability in the treatment of depression of liver stagnation and spleen deficiency, but there is no evidence for clinical efficacy and safety. Therefore, this study aims to integrate the clinical related syndromes of direct and indirect comparison by using systematic evaluation and network meta-analysis (NMA). According to the data, the different Chinese patent medicines with the same evidence body for the treatment of the disease are collected, analyzed, and sequenced in a quantitative and comprehensive way, and then the advantages and disadvantages of the efficacy and safety between different Chinese patent medicines are screened out to get the best choice scheme, thus providing reference value and evidence-based theoretical evidence for the clinical optimization of drug selection. METHODS: Comprehensive retrieval of China National Knowledge Infrastructure, Chinese scientific journal database (VIP), China biological feature database (CBM) and WANFANG Data Chinese electronic database and the Cochrane Library, PubMed, Web of Science, and EMBASE foreign database. Search and publish the clinical randomized controlled trials of these 3 Chinese patent medicines combined with fluoxetine compared with fluoxetine. The retrieval time is from the establishment of the database to October 31, 2019. The 2 first authors will screen the literatures that meet the inclusion criteria, extract the data independently according to the predesigned rules, and evaluate the literature quality and bias risk of the included research according to the Cochrane 5.1 manual standard. R and Aggregate Data Drug Information System software were used for data consolidation and NMA to evaluate the ranking probability of all interventions. RESULTS: This result will show that the best oral Chinese patent medicine to assist the treatment of liver stagnation and spleen deficiency depression provides reliable evidence. CONCLUSION: This study will provide systematic evidence-based medicine evidence for TCM assisted treatment of depression of liver stagnation and spleen deficiency type, and help clinicians, patients with depression and decision-makers to make more effective, safer, and economic optimal treatment plan in the decision-making process. PROSPERO REGISTRATION NUMBER: CRD42019115695.

8.
Infect Immun ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31932328

RESUMO

Streptococcus suis serotype 2 is an important bacterial pathogen of swine and also an emerging zoonotic agent that may be harmful to human health. Although the virulence genes of S. suis have been extensively studied, the mechanisms by which they damage the central immune organs have rarely been studied. In the current work, we wanted to uncover more details about the impact and mechanisms of S. suis on specific populations of thymic and immune cells in infected mice. Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assays revealed that S. suis infection induced apoptosis in CD3+, CD14+ and epithelial cells from the thymus. S. suis infection resulted in a rapid depletion of mitochondrial permeability and release of cytochrome c (CytC) and apoptosis-inducing factor (AIF) through up-regulation of Bax and down-regulation of Bcl-xl and Bcl2 expression in thymocytes. Moreover, S. suis infection increased cleavage of caspase-3, -8, -9. Thus, S. suis induced thymocyte apoptosis through a p53- and caspase-dependent pathway, which led to a decrease of CD3+ cells in the thymus, subsequently decreasing the numbers of CD4+ and CD8+ cells in the peripheral blood. Finally, expression dysregulation of pro-inflammatory cytokines in the serum including interleukin (IL)-2, IL-6, IL-12 (p70), tumor necrosis factor (TNF), and IL-10 was observed in mice after S. suis type 2 infection. Taken together, these results suggest that S. suis infection can cause atrophy of the thymus and induces apoptosis of thymocytes in mice, thus likely suppressing host immunity.

9.
Food Chem ; 313: 126161, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31931420

RESUMO

The effects of fermentation on the dynamic state of proton populations, digestibility, and functional properties of potato flours prepared from Atlantic and Kexin No. 1 were investigated. Depending on the changes in moisture content and digestibility of potato flours during fermentation, three proton populations were distinguished. Populations P21 and P22 of fermented potato flours gradually increased during fermentation, resistant starch content, swelling power, and pasting properties also increased, whereas moisture content, digestible starch content, and water solubility index gradually decreased. Correlation analyses showed that populations P21 and P22 had significantly positive correlations with resistant starch content, swelling power, peak viscosity, and breakdown viscosity of fermented potato flours (p < 0.05), while population P23 had significantly positive correlations with moisture content, digestible starch content, and water solubility index (p < 0.001). The results implied that the proton population was an important factor influencing the digestibility and functional properties of fermented potato flours.

10.
Biogerontology ; 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31960183

RESUMO

Coix seed oil (CSO) has many beneficial effects, but there is limited research on its influence on the processes and mechanisms related to senescence. Here, we used Caenorhabditis elegans as an in vivo model to investigate CSO's bioeffects on longevity. CSO (1 mg/mL) significantly extended the mean lifespan of C. elegans by over 22.79% and markedly improved stress resistance. Gene-specific mutant studies showed that the CSO-mediated increase in life expectancy was dependent on mev-1, hsf-1 and daf-16, but not daf-2. Furthermore, CSO significantly upregulated stress-inducible genes, including daf-16 and its downstream genes (sod-3, hsp-16.2 and gst-4). In addition, four major fatty acids, linoleic, oleic, palmitic and stearic, played leading roles in C. elegans' extended lifespan. Thus, CSO increased the life expectancy of, and enhanced the stress resistance in, C. elegans mainly through daf-16 and its downstream genes, but not through the insulin/insulin-like growth factor 1 signaling pathway.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31917615

RESUMO

RATIONALE: Vascular remodeling, including smooth muscle cell hypertrophy and proliferation, is the key pathological feature of pulmonary arterial hypertension (PAH). Prostaglandin (PG) I2 analogs (baraprost, iloprost, and treprostinil) are effective in the treatment of PAH. Of note, the clinically favorable effects of treprostinil in severe PAH may be attributable to concomitant activation of PGD2 receptor subtype 1 (DP1). OBJECTIVES: To study the role of DP1 in the progression of PAH and its underlying mechanism. METHODS AND RESULTS: DP1 expression was downregulated in hypoxia-treated PASMCs and in pulmonary arteries (PAs) from rodent PAH models and idiopathic PAH patients. DP1 deletion exacerbated PA remodeling in hypoxia-induced PAH, whereas pharmacological activation or forced expression of DP1 receptor had the opposite effect in different rodent models. DP1 deficiency promoted PASMC hypertrophy and proliferation in response to hypoxia via induction of mammalian target of rapamycin complex (mTORC) 1 activity. Rapamycin, an inhibitor of mTORC1, alleviated the hypoxia-induced exacerbation of PAH in DP1-/- mice. DP1 activation facilitated raptor dissociation from mTORC1 complex and suppressed mTORC1 activity through protein kinase A (PKA)-dependent phosphorylation of raptor at Ser791. Moreover, treprostinil treatment blocked the progression of hypoxia-induced PAH in mice in part by targeting DP1 receptor. CONCLUSION: DP1 activation attenuates hypoxia-induced PA remodeling and PAH through PKA-mediated dissociation of raptor from the mTORC1 complex. These results suggest that DP1 receptor may serve as a therapeutic target for the management of PAH.

12.
Circulation ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31893939

RESUMO

Background: Blood pressure (BP) often rises with aging, but exact mechanisms are still not completely understood. With aging, the level of pro-inflammatory cytokines increases in T lymphocyte. Prostaglandin (PG) D2, a pro-resolution mediator, suppresses Th1 cytokines through D-prostanoid receptor 1 (DP1). In this study, we aimed to investigate the role of PGD2/DP1 axis in T cells on age-related hypertension. Methods: To clarify the physiological and pathophysiological roles of DP1 in T cells with aging, peripheral blood samples were collected from young and older male participants and CD4+ T cells were sorted for gene expression, PG production and western bot assays. Mice BP was quantified by invasive telemetric monitor. Results: PGD2/DP1 axis was down-regulated in CD4+ T cells from older humans and aged mice, DP1 deletion in CD4+ T cells (TDP1KO) augmented age-related hypertension in aged male mice by enhancing Th1 cytokine secretion, vascular remodeling and CD4+ T cells infiltration, superoxide production in vasculature and kidneys. Conversely, forced expression of exogenous DP1 in T cells retarded age-associated hypertension in mice by reducing Th1 cytokine secretion. TNFα neutralization or IFNγ deletion ameliorated the age-related hypertension in TDP1KO mice. Mechanistically, DP1 inhibited Th1 activity via the Gαs/PKA/p-Sp1/NEDD4L pathway-mediated T-bet ubiquitination. T-bet deletion or forced NEDD4L expression in CD4+ T cell attenuated age-related hypertension in TDP1KO mice. DP1 receptor activation by BW245C prevented age-associated BP elevation and reduced vascular/renal superoxide production in male mice. Conclusions: PGD2/DP1 axis suppresses age-related Th1 activation and subsequent hypertensive response in male mice through increase of NEDD4L-mediated T-bet degradation by ubiquitination. Therefore, the T cell DP1 receptor may be an attractive therapeutic target for age-related hypertension in males.

13.
Arch Microbiol ; 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31894394

RESUMO

Network analysis has contributed to studies of the interactions of microorganisms and the identification of key populations. However, such analysis has rarely been conducted in the study of reservoir bacterioplankton communities. This study investigated the bacterioplankton community composition in the surface water of the Danjiangkou Reservoir using the Illumina MiSeq sequencing platform. We observed that the bacterioplankton community primarily consisted of 27 phyla and 336 genera, including Actinobacteria, Proteobacteria, and Bacteroidetes, demonstrating the richness of the community composition. Redundancy analysis of the bacterioplankton communities and environmental variables showed that the total nitrogen (TN), pH, chemical oxygen demand (COD), and permanganate index (CODMn) were important factors affecting the bacterioplankton distribution. Network analysis was performed using the relative abundances of bacterioplankton based on the phylogenetic molecular ecological network (pMEN) method. The connectivity of node i within modules (Zi), the connectivity of node i among modules (Pi), and the number of key bacteria were high at the Taizishan and Heijizui sites, which were associated with higher TN contents than at the other sites. Among the physicochemical properties of water, TN, ammonia nitrogen (NH4-N), pH, COD, and dissolved oxygen (DO) might have great influences on the functional units of the bacterial communities in bacterioplankton molecular networks. This study improves the understanding of the structure and function of bacterioplankton communities in the Danjiangkou Reservoir.

14.
ACS Appl Mater Interfaces ; 12(5): 6276-6282, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31937099

RESUMO

Molybdenum disulfide (MoS2) with excellent properties has been widely reported in recent years. However, it is a great challenge to achieve p-type conductivity in MoS2 because of its native stubborn n-type conductivity. Substitutional transition metal doping has been proved to be an effective approach to tune their intrinsic properties and enhance device performance. Herein, we report the growth of Nb-doping large-area monolayer MoS2 by a one-step salt-assisted chemical vapor deposition method. Electrical measurements indicate that Nb doping suppresses n-type conductivity in MoS2 and shows an ambipolar transport behavior after annealing under the sulfur atmosphere, which highlights the p-type doping effect via Nb, corresponding to the density functional theory calculations with Fermi-level shifting to valence band maximum. This work provides a promising approach of two-dimensional materials in electronic and optoelectronic applications.

15.
Sci Total Environ ; 709: 136171, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31905582

RESUMO

Biochar-mineral (bentonite/calcite) composite (BC-CM) prepared at different temperatures were tested under varied conditions for effective removal of lead (Pb) from aqueous solution. With increasing pyrolysis temperature, increased surface area, pore volume, bentonite decomposition and less or no decomposition of calcite occurred. Bentonite-biochar (BCS) and calcite-biochar (CCS) prepared at 700 °C were found most suitable for efficient removal of Pb (99.9%). Bentonite and calcite acted as catalyst and contributed to changes in yield, pH, texture, functional groups, minerals and carbonization that facilitated efficient Pb removal by BCS 700 and CCS 700. Pb concentration, pH, dose of BCS and CCS, and contact time were further optimized using response surface methodology (RSM) for maximizing removal percentage (R%) of Pb and adsorption capacity (qt). Both BCS 700 and CCS 700 showed similar effects (positive/negative) of factors on R% and qt. Under optimized conditions, 0.21 g of BCS 700 effectively removed 99.2% of 431 mg/L in 3.6 h at solution pH of 4.2, while 0.07 g CCS 700 removed 97.06% of 232 mg/L in 3.5 h at 5.5 pH. Removal of Pb onto both BCS and CCS was by monolayer adsorption with maximum adsorption capacity of 500 mg/g. Rapid Pb removal was observed within 2 h of contact time (CCS 700 > BCS 700) and equilibrium was achieved within 10 h. BCS 700 followed first order and CCS 700 followed second order kinetic model. Electrostatic attraction between Pb ions and mineral groups present in BCS 700 and CCS 700 also played important role in Pb removal. This study clearly demonstrated that composite of biochar with bentonite or calcite under optimized conditions significantly improved Pb removal and adsorption capacity that can be further utilized for larger scale applications.

16.
Sci Total Environ ; 712: 136495, 2020 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-31945536

RESUMO

Long-term overgrazing tends to cause soil phosphorus (P) deficiency in grasslands. The relationships between grazing-induced shifts in species composition and root traits associated with P acquisition involved in these shifts remain unknown. Species vary in their P-acquisition strategies, and we hypothesize that species that acquire P more efficiently are better adapted to overgrazing. We measured relative biomass, root physiological activities (e.g., rhizosheath acid phosphatase activity (Apase), and leaf manganese concentration ([Mn]) as a proxy for carboxylate concentrations in rhizosheath) and morphological traits (e.g., specific root length) of six common species in a field experiment conducted in a typical steppe of Inner Mongolia. There were two exclosure demonstration plots, i.e. exclosed since 1983 and 1996, and long-term free grazing without exclosure of sheep. Long-term overgrazing caused a reduction in bulk soil Olsen P concentration and increased community-weighted leaf nitrogen: P ratio by 27% and 37%, respectively, indicating more severe P limitation for steppe vegetation. Carex duriuscula exhibited an inherently greater specific root length, proportion of fine roots and rhizosheath Apase than other species did in both exclosure and grazing treatments. Cleistogenes squarrosa showed a greater leaf [Mn] induced by overgrazing. The increased dominance of C. duriuscula and C. squarrosa was positively correlated with finer roots, greater rhizosheath Apase or carboxylate release under long-term overgrazing. Species that had inefficient root traits for P acquisition (e.g., low specific root length and low leaf [Mn]), i.e. Stipa grandis, exhibited a decreased dominance in response to overgrazing. Dominance of species did not change under grazing which may be related to either relatively inefficient inherent morphological (i.e. in Artemisia frigida) or physiological traits (i.e. in Leymus chinensis and Agropyron michnoi) for P acquisition. Our study highlights the importance of acknowledging root traits involved in efficient P acquisition for theories on community succession induced by overgrazing.

17.
Ann Vasc Surg ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31911130

RESUMO

BACKGROUND: To develop and verify a risk predictive model/scoring system for pulmonary embolism (PE) among hospitalized patients with deep venous thrombosis of the lower extremities (LDVT). METHODS: 776 patients with LDVT were enrolled in a case-control study between January 2016 and June 2017 from the Vascular Surgery Department of Shanxi Dayi Hospital, China. They were randomly divided into development (543 patients, 70%) and validation (233 patients, 30%) databases. Based on the results of pulmonary computed tomography arteriography, patients were divided into 2 categories; those with PE were designated as the case group, whereas those without comprised the controls. A logistic regression model and scoring system for PE in patients with LDVT was established in the development database and verified in the validation database. Scoring system (Shanxi Dayi Hospital score [SDH score]) was tabulated as follows: right lower extremity or bilateral lower extremities, 1; surgery or immobilization, 1; malignant tumor, 1; history of venous thromboembolism (VTE), 2; D-dimer >1,000 ng/mL, 2; and unprovoked, 2. Calibration and discrimination of the model were assessed by the Hosmer-Lemeshow goodness of fit test and the area under the receiver operating characteristic curve (AUC). Wells score, the Revised Geneva score, and the SDH score for predictive value of PE by AUC in the validation database were compared. RESULTS: 776 patients with LDVT were divided into 2 risk categories based on the scores from the risk model as follows: PE unlikely (score <3) and PE likely (score ≥3). Sensitivity, specificity, and crude agreement of the SDH score in the development database were 76.39%, 55.89%, and 61.33%, respectively. In the validation database, the logistic regression model showed good calibration and discriminative power. The Hosmer-Lemeshow goodness of fit test P value was >0.05, and the AUC was 0.705 (95% CI: 0.634-0.776, P < 0.001). The SDH score also showed good discriminative power, and the AUC was 0.702 (95% CI: 0.631-0.774, P < 0.001). Sensitivity, specificity, and crude agreement of the SDH score in the validation database were 67.61%, 61.73%, and 63.52%, respectively. AUC for the Wells score and the Revised Geneva score was 0.611 (95% CI: 0.533-0.688, P = 0.007) and 0.585 (95% CI: 0.503-0.666, P = 0.040), respectively. Difference of the AUC was not statistically significant between the Wells score and the SDH score (0.611 vs. 0.702, P = 0.059) but was so between the Revised Geneva score and the SDH score (0.585 vs. 0.702, P = 0.016). Sensitivity of the Wells score, Revised Geneva score, and the SDH score (64.79%, 67.61% vs. 67.61%) was not statistically significant. However, the specificity of the Wells score and Revised Geneva score was significantly lower than that of the SDH score (48.77%, 39.51% vs. 61.73%). CONCLUSIONS: Our logistic regression model and the SDH score based on 7 risk factors as right lower extremity, bilateral lower extremities, unprovoked, surgery or immobilization, malignant tumor, history of VTE, and D-dimer>1,000 ng/mL showed good calibration and discriminative power for the assessment of PE risk in patients with LDVT. The SDH score is more specific for PE prediction in the Chinese population, compared with the Wells score and the Revised Geneva score.

18.
Int J Biol Macromol ; 147: 453-462, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31923519

RESUMO

Due to the favorable stability, water solubility and good biocompatibility, carbon dots have attracted much attention. Herein, a novel nitrogen-doping bifunctional carbon dots (N-BCDs) with ultra-highly quantum yield (QYabs = 70.4%) is prepared through microwave-assisted method. 50 µg/mL of N-BCDs emit intense fluorescence in HeLa and GES-1 cells with negligible cytotoxicity. In addition, effective inhibition of N-BCDs to human insulin (HI) fibrillation is observed even at 10:1 (mass ratio of HI: N-BCDs) by ThT fluorescence, CD assay and TEM. N-BCDs prevent HI from fibrillation with prolonged lag time and reduced fluorescent intensity at equilibrium, regardless of the addition time of N-BCDs (HI: N-BCDs = 1:1, mass ratio), which has been rarely reported before. Furthermore, the morphology of final HI fibrils is shorter and thinner in the presence of N-BCDs. Mechanism studies reveal that the enhanced hydrogen bond between HI monomers and N-BCDs inhibits nucleation during the lag stage (Ka: 1.54 × 104 L·mol-1, 298 K), while the accumulation of N-BCDs blocks the growth of profibrils in the elongation stage. To the best of our knowledge, it's the first time to observe the accumulation of N-BCDs around HI profibrils with TEM. Our study provides a new strategy for developing efficient nanoparticle inhibitors for protein fibrillation.

19.
Bioresour Technol ; 301: 122774, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31954973

RESUMO

The thermal characteristics of Actinobacillus succinogenes (AS) from pyrolysis, torrefaction, and combustion are analyzed to evaluate the potential of this biomass as a renewable fuel. AS pyrolysis can be classified into four stages, and its main decomposition zone is at 200-500 °C. The solid yield of AS after 60 min torrefaction is over 60 wt%, and the torrefaction severity index map indicates that a high torrefaction temperature with a short duration has a more profound influence on its decomposition. The Py-GC/MS analysis of AS suggests that the volatile products from 500 °C pyrolysis are similar to microalgae-derived pyrolysis bio-oils. The combustibility index (S) of AS is 4.07 × 10-7 which is much higher than that of lignite coal (0.39 × 10-7) and bituminous coal (0.18 × 10-7), and close to those of biochar and bio-oil. The obtained results are conducive to the development of microorganisms as fuel to achieve a circular bioeconomy.


Assuntos
Actinobacillus , Microalgas , Biomassa , Temperatura Alta
20.
J Clin Nurs ; 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31970842

RESUMO

AIMS AND OBJECTIVES: The primary aim of this study is to explore the influence of obesity, healthy lifestyle and sleep quality of endometrial cancer survivors on their fatigue level. BACKGROUND: As many as 30% of endometrial cancer survivors still suffer from fatigue 5 years after completing therapy. Fatigue may hinder cancer survivors from participating in daily activities or returning to their original roles and functions, thus affecting their health-related quality of life. DESIGN: This study adopted a cross-sectional correlational research design. The STROBE checklist for cross-sectional studies was used as a reference for reporting the study. METHODS: A consecutive sample of 134 endometrial cancer survivors was recruited from the outpatient clinics of a medical centre in Taipei, Taiwan. Data were collected using structured questionnaires. RESULTS: Study subjects scored 44 points (SD = 7.09) on average for the fatigue levels. Results of linear regression showed that sleep quality (ß = -0.38), comorbidity index (ß = -0.024) and age (ß = 0.20) were important predictors of fatigue. However, differences in obesity, vegetable and fruit intake, physical activity did not lead to significant differences in fatigue level. CONCLUSIONS: Survivors who had poorer sleep quality, higher comorbidity index and younger age reported higher fatigue. RELEVANCE TO CLINICAL PRACTICE: The study findings are relevant for assessing and preventing fatigue in endometrial cancer survivors. Those with poorer sleep quality, higher comorbidity index and younger age are at a greater risk for fatigue and deserve further attention. Although the study results failed to support the link between obesity, vegetable and fruit intake, physical activity and fatigue, the ratio of survivors who comply with recommended healthy lifestyles was low. Hence, it is of urgent necessity that this population receives the help to maintain a healthy lifestyle.

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