Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 52
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Int J Biol Macromol ; 142: 395-403, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622707

RESUMO

Zwitterionic polymers have attracted great attention due to their unique structure, which can be used for various applications, such as the improvement of nanoparticle stability, ion exchange, sewage treatment, and biomedicine. Here, zwitterionic functionalized starch nanoparticles (SNPs) were successfully prepared in two steps by carboxymethylation and quaternization. The structure of the modified SNPs was confirmed using Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and elemental analysis. The zwitterionic SNPs with a size of 30-100 nm were well dispersed without aggregation. The relative crystallinity of zwitterionic SNPs (35.2%) was increased compared to that of SNPs (31.7%). The zwitterionic SNPs could be used effectively to absorb dyes, and its isoelectric point was around pH 9.4. The zwitterionic SNPs may be used as nontoxic adsorption carriers that can be used in health foods or drug delivery applications.

2.
Allergol. immunopatol ; 47(6): 585-590, nov.-dic. 2019. graf
Artigo em Inglês | IBECS-Express | ID: ibc-ET2-3843

RESUMO

Introduction and objectives: Transforming growth factor β1 (TGFβ1) and dysregulated microRNA-21 (miR-21) expression is associated with TGFβ/Smad signaling pathway activation and fibrosis. While calcitriol has been shown to improve airway remodeling in asthmatic mice, its mechanism remains unknown. In this study, the effect of calcitriol on the TGFβ/Smad signaling pathway and miR-21 expression in human bronchial fibroblasts was investigated to explore the mechanism of action of calcitriol and the inhaled glucocorticoid, budesonide, in airway remodeling. Materials and methods: Human bronchial fibroblasts were pretreated with budesonide, calcitriol, or budesonide plus calcitriol, and stimulated with TGFβ1 for 48h. Quantitative real-time PCR was used to determine the expression of miR-21. Western blot was used to determine airway remodeling-related proteins, TGFβ/Smad signaling pathway-related proteins, glucocorticoid receptor, and vitamin D receptor (VDR) expression. Results: Both budesonide and calcitriol down-regulated miR-21 expression in human bronchial fibroblasts, up-regulated Smad7 expression, and inhibited the expression of airway remodeling-related proteins. Both budesonide and calcitriol up-regulated the low expression of VDR induced by TGFβ1 in human bronchial fibroblasts. The expression of VDR in the combined treatment group (budesonide plus calcitriol) was significantly higher than that in the calcitriol treatment group. The expression of collagen type I in the combined treatment group was significantly lower than that in the calcitriol treatment group. Conclusions: Calcitriol can up-regulate the expression of VDR in human bronchial fibroblasts and exert an anti-airway remodeling effect. Budesonide can up-regulate the expression of VDR in human bronchial fibroblasts and enhance the inhibitory effect of calcitriol on airway remodeling


No disponible

3.
Nat Commun ; 10(1): 5013, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31676774

RESUMO

For atomically thin two-dimensional materials, interfacial effects may dominate the entire response of devices, because most of the atoms are in the interface/surface. Graphene/sapphire has great application in electronic devices and semiconductor thin-film growth, but the nature of this interface is largely unknown. Here we find that the sapphire surface has a strong interaction with some of the carbon atoms in graphene to form a C-O-Al configuration, indicating that the interface interaction is no longer a simple van der Waals interaction. In addition, the structural relaxation of sapphire near the interface is significantly suppressed and very different from that of a bare sapphire surface. Such an interfacial C-O-Al bond is formed during graphene growth at high temperature. Our study provides valuable insights into understanding the electronic structures of graphene on sapphire and remote control of epitaxy growth of thin films by using a graphene-sapphire substrate.

4.
Small ; : e1902844, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31490630

RESUMO

Bilayer or few-layer 2D materials showing novel electrical properties in electronic device applications have aroused increasing interest in recent years. Obtaining a comprehensive understanding of interlayer contact conductance still remains a challenge, but is significant for improving the performance of bilayer or few-layer 2D electronic devices. Here, conductive atomic force microscope (C-AFM) experiments are reported to explore the interlayer contact conductance between bilayer graphene (BLG) with various twisted stacking structures fabricated by the chemical vapor deposition (CVD) method. The current maps show that the interlayer contact conductance between BLG strongly depends on the twist angle. The interlayer contact conductance of 0° AB-stacking bilayer graphene (AB-BLG) is ≈4 times as large as that of 30° twisted bilayer graphene (t-BLG), which indicates that the twist angle-dependent interlayer contact conductance originates from the coupling-decoupling transitions. Moreover, the moiré superlattice-level current images of t-BLG show modulations of local interlayer contact conductance. Density functional theory calculations together with a theoretical model reproduce the C-AFM current map and show that the modulation is mainly attributed to the overall contribution of local interfacial carrier density and tunneling barrier.

5.
Adv Mater ; 31(43): e1902978, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31502709

RESUMO

Contamination is a major concern in surface and interface technologies. Given that graphene is a 2D monolayer material with an extremely large surface area, surface contamination may seriously degrade its intrinsic properties and strongly hinder its applicability in surface and interfacial regions. However, large-scale and facile treatment methods for producing clean graphene films that preserve its excellent properties have not yet been achieved. Herein, an efficient postgrowth treatment method for selectively removing surface contamination to achieve a large-area superclean graphene surface is reported. The as-obtained superclean graphene, with surface cleanness exceeding 99%, can be transferred to dielectric substrates with significantly reduced polymer residues, yielding ultrahigh carrier mobility of 500 000 cm2 V-1 s-1 and low contact resistance of 118 Ω µm. The successful removal of contamination is enabled by the strong adhesive force of the activated-carbon-based lint roller on graphene contaminants.

6.
Allergol Immunopathol (Madr) ; 47(6): 585-590, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31204163

RESUMO

INTRODUCTION AND OBJECTIVES: Transforming growth factor ß1 (TGFß1) and dysregulated microRNA-21 (miR-21) expression is associated with TGFß/Smad signaling pathway activation and fibrosis. While calcitriol has been shown to improve airway remodeling in asthmatic mice, its mechanism remains unknown. In this study, the effect of calcitriol on the TGFß/Smad signaling pathway and miR-21 expression in human bronchial fibroblasts was investigated to explore the mechanism of action of calcitriol and the inhaled glucocorticoid, budesonide, in airway remodeling. MATERIALS AND METHODS: Human bronchial fibroblasts were pretreated with budesonide, calcitriol, or budesonide plus calcitriol, and stimulated with TGFß1 for 48h. Quantitative real-time PCR was used to determine the expression of miR-21. Western blot was used to determine airway remodeling-related proteins, TGFß/Smad signaling pathway-related proteins, glucocorticoid receptor, and vitamin D receptor (VDR) expression. RESULTS: Both budesonide and calcitriol down-regulated miR-21 expression in human bronchial fibroblasts, up-regulated Smad7 expression, and inhibited the expression of airway remodeling-related proteins. Both budesonide and calcitriol up-regulated the low expression of VDR induced by TGFß1 in human bronchial fibroblasts. The expression of VDR in the combined treatment group (budesonide plus calcitriol) was significantly higher than that in the calcitriol treatment group. The expression of collagen type I in the combined treatment group was significantly lower than that in the calcitriol treatment group. CONCLUSIONS: Calcitriol can up-regulate the expression of VDR in human bronchial fibroblasts and exert an anti-airway remodeling effect. Budesonide can up-regulate the expression of VDR in human bronchial fibroblasts and enhance the inhibitory effect of calcitriol on airway remodeling.

7.
Microsc Microanal ; 25(3): 786-797, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30898184

RESUMO

This work focuses on the application of nanoindentation measurements and the finite element method for analyzing the mechanical properties of the rostrum of the outstanding driller Cyrtotrachelus buqueti Guer. Nanoindentation tests were carried out to measure the Young's modulus and hardness of the rostrum, with the results for the "dry" samples being 13.886 ± 0.75 and 0.368 ± 0.0445 GPa, respectively. The values for the "fresh" samples showed no clear difference from those of the "dry" ones. Moreover, field observation was conducted to determine the motion behaviors of the rostrum on the weevil. Micro-computed tomography technology was employed to obtain structural information about the rostrum, using 9 µm slices. A real three-dimensional model of the rostrum was created using the MIMICS application. Finally, the mechanical properties of the rostrum were determined by finite element analysis. It was concluded that the rostrum provides an ideal biological template for the design of biocomposite materials and lightweight tube-shaped structures. The properties determined in this study can potentially be applied in different fields, such as in the design of automotive hybrid transmission shafts, helicopter tail drive shafts, robotic arms, and other sandwich structures in aerospace engineering.

8.
J Asthma ; 56(4): 341-349, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29621415

RESUMO

OBJECTIVE: Airway remodeling is an important pathological feature of asthma. Excessive deposition of extracellular matrix (e.g., collagen) secreted from fibroblasts is a major factor contributing to airway remodeling. Currently, the mechanism by which collagen continues to be oversynthesized in the airway remains unclear. In this study, we investigated the role of the microRNA-21 (miR-21) and TGFß/Smad signaling pathway in human bronchial fibroblasts (HBFs), and explored the regulatory mechanism of airway remodeling. METHODS: HBFs were cultured in vitro and treated with the transforming growth factor ß (TGFß), receptor inhibitor (SB431542), and TGFß1. miR-21 and Smad7 overexpressing lentiviruses, as well as an miR-21 interfering lentivirus were constructed and transfected into HBFs. Western blotting was used to determine the expression of airway remodeling-related proteins and proteins in the TGFß/Smad signaling pathway. miR-21 expression was measured by quantitative real-time PCR. RESULTS: The high expression of miR-21 induced by TGFß1 was reduced following the treatment with the SB431542 in HBFs. Smad7 overexpression inhibited the elevated expression of the COL I protein induced by miR-21 overexpression in HBFs. Inhibiting miR-21 expression upregulated the level of Smad7 protein, thus reducing the expression of airway remodeling-related proteins induced by TGFß1 stimulation in HBFs. CONCLUSIONS: TGFß1 can induce miR-21 expression in HBFs through the TGFß/Smad signaling pathway to promote airway remodeling. miR-21 downregulates Smad7, activates the TGFß/Smad signaling pathway, and promotes airway remodeling. Mutual regulation between miR-21 and the TGFß/Smad signaling pathway in HBFs promotes airway remodeling.

9.
Artigo em Inglês | MEDLINE | ID: mdl-29974192

RESUMO

Sprawling posture vertebrates have a flexible spine that bends the trunk primarily in the horizontal plane during locomotion. By coordinating cyclical lateral trunk flexion and limb movements, these animals are very mobile and show extraordinary maneuverability. The dynamic and static stability displayed in complex and changing environments are highly correlated with such lateral bending patterns. The axial dynamics of their compliant body can also be critical for achieving energy-efficient locomotion at high velocities. In this paper, lateral undulation is used to characterize the bending pattern. The production of ground reaction forces (GRFs) and the related center of mass (COM) dynamics during locomotion are the fundamental mechanisms to be considered. Mainly based on research on geckos, which show unrestricted movement in three-dimensional space, we review current knowledge on the trunk flexibility and waveforms of lateral trunk movement. We investigate locomotion dynamics and mechanisms underlying the lateral undulation pattern. This paper also provides insights into the roles of this pattern in obtaining flexible and efficient walking, running, and climbing. Finally, we discuss the potential application of lateral undulation patterns to bio-inspired robotics.


Assuntos
Movimento , Postura , Coluna Vertebral , Animais , Fenômenos Biomecânicos , Lagartos/fisiologia , Movimento/fisiologia , Postura/fisiologia , Coluna Vertebral/fisiologia
10.
Can Respir J ; 2018: 5259240, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29854030

RESUMO

Background and Objective: While calcitriol can inhibit airway remodeling in asthmatic mice, the mechanism remains unclear. The purpose of this study was to explore the mechanism of action of calcitriol on airway remodeling in asthma and its interaction with budesonide. Methods: A mouse model of asthma was established by allergic sensitization and challenge with ovalbumin. The mice were treated with budesonide, calcitriol, or budesonide plus calcitriol. The expression of airway remodeling-related proteins, transforming growth factor ß (TGFß) signaling pathway-related proteins, the glucocorticoid receptor, and vitamin D receptor (VDR) was determined by immunohistochemical staining and Western blot analysis. Quantitative real-time PCR was used to determine the expression of microRNA-21 (miR-21) in the lung tissue of mice. Results: Monotherapy with budesonide or calcitriol inhibited the high expression of collagen type I protein and upregulated the low expression of Smad7 in asthmatic mice. There was a synergistic interaction between budesonide and calcitriol in combined treatment. The expression of miR-21 in the combined treatment group was significantly lower than that in the calcitriol treatment group. VDR expression in the combined treatment group was significantly higher than that of the calcitriol treatment group. Conclusion: Budesonide and calcitriol have a synergistic effect on airway remodeling in asthmatic mice.


Assuntos
Remodelação das Vias Aéreas/efeitos dos fármacos , Anti-Inflamatórios/uso terapêutico , Budesonida/uso terapêutico , Calcitriol/uso terapêutico , Hormônios e Agentes Reguladores de Cálcio/uso terapêutico , Animais , Anti-Inflamatórios/farmacologia , Asma/tratamento farmacológico , Asma/metabolismo , Budesonida/farmacologia , Calcitriol/farmacologia , Hormônios e Agentes Reguladores de Cálcio/farmacologia , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Sinergismo Farmacológico , Feminino , Camundongos Endogâmicos BALB C , MicroRNAs/metabolismo , Ovalbumina , Distribuição Aleatória , Receptores de Calcitriol/metabolismo , Receptores de Glucocorticoides/metabolismo , Receptores X Retinoide/metabolismo , Proteínas Smad/metabolismo , Fator de Crescimento Transformador beta/metabolismo
11.
Sci Rep ; 7(1): 10261, 2017 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-28860547

RESUMO

Altered mean platelet volume (MPV) is implicated in several malignancies. However, the clinicopathological significance and prognostic value of MPV in colorectal cancer (CRC) is still elusive. The purpose of this study was to elucidate the predictive significance of MPV in CRC. The retrospective study recruited 509 consecutive CRC patients between January 2009 and December 2009. The relationships between MPV and clinicopathological characteristics were analyzed. Kaplan-Meier method and Cox regression were used to evaluate the prognostic impact of MPV. Of the 509 CRC patients, high MPV levels were detected in 150 (29.5%) patients. Elevated MPV was associated with tumor differentiation (p < 0.001). Patients with increased MPV had poor overall survival compared with those with normal level (60.0% vs. 83.6%, log-rank test, p = 0.035). Cox regression analysis showed that MPV was an independent prognostic factor in CRC (HR = 1.452, 95% CI = 1.118-1.884, p = 0.005). In conclusion, MPV is easily available in routine blood test. Elevated MPV might act as a marker of prognosis and therapeutic target for CRC.


Assuntos
Neoplasias Colorretais/sangue , Neoplasias Colorretais/mortalidade , Volume Plaquetário Médio , Idoso , Biomarcadores , Neoplasias Colorretais/patologia , Feminino , Seguimentos , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Estadiamento de Neoplasias , Contagem de Plaquetas , Prognóstico , Modelos de Riscos Proporcionais , Curva ROC
12.
Prev Chronic Dis ; 14: E61, 2017 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-28749775

RESUMO

From January through December 2015, the California Department of Health Care Services, which administers Medi-Cal, the nation's largest Medicaid program, conducted a quality improvement collaborative (QIC) with 9 Medi-Cal managed care plans (MCPs) aimed at improving hypertension control consistent with the Million Hearts initiative. The QIC included quarterly webinars and links to local, state, and national resources that consisted of materials and consultations with subject matter experts. Participating MCPs demonstrated an average increase of 5.0 percentage points in their rates of controlled hypertension. Collaboratives can achieve substantial quality improvement in Medicaid managed care plans.


Assuntos
Hipertensão/epidemiologia , Hipertensão/prevenção & controle , Pobreza , Anti-Hipertensivos , California/epidemiologia , Acesso aos Serviços de Saúde , Humanos , Hipertensão/tratamento farmacológico , Programas de Assistência Gerenciada , Medicaid , Melhoria de Qualidade , Planos Governamentais de Saúde , Estados Unidos
13.
Nat Commun ; 8: 14029, 2017 02 14.
Artigo em Inglês | MEDLINE | ID: mdl-28195130

RESUMO

Superlubricity of graphite and graphene has aroused increasing interest in recent years. Yet how to obtain a long-lasting superlubricity between graphene layers, under high applied normal load in ambient atmosphere still remains a challenge but is highly desirable. Here, we report a direct measurement of sliding friction between graphene and graphene, and graphene and hexagonal boron nitride (h-BN) under high contact pressures by employing graphene-coated microsphere (GMS) probe prepared by metal-catalyst-free chemical vapour deposition. The exceptionally low and robust friction coefficient of 0.003 is accomplished under local asperity contact pressure up to 1 GPa, at arbitrary relative surface rotation angles, which is insensitive to relative humidity up to 51% RH. This ultralow friction is attributed to the sustainable overall incommensurability due to the multi-asperity contact covered with randomly oriented graphene nanograins. This realization of microscale superlubricity can be extended to the sliding between a variety of two-dimensional (2D) layers.

14.
Mol Cancer ; 16(1): 9, 2017 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-28086904

RESUMO

BACKGROUND: With more than 600,000 mortalities each year, colorectal cancer (CRC) is the third most commonly diagnosed type of cancer worldwide. Recently, mechanisms involving noncoding RNAs have been implicated in the development of CRC. METHODS: We examined expression levels of lncRNA CRNDE and miR-181a-5p in 64 cases of CRC tissues and cell lines by qRT-PCR. Gain-of-function and loss-of-function assays were performed to examine the effect of CRNDE and miR-181a-5p on proliferation and chemoresistance of CRC cells. Using fluorescence reporter and western blot assays, we also explored the possible mechanisms of CRNDE in CRC cells. RESULTS: In this study, we found that the expression levels of the CRNDE were upregulated in CRC clinical tissue samples. We identified microRNA miR-181a-5p as an inhibitory target of CRNDE. Both CRNDE knockdown and miR-181a-5p overexpression in CRC cell lines led to inhibited cell proliferation and reduced chemoresistance. We also determined that ß-catenin and TCF4 were inhibitory targets of miR-181a-5p, and that Wnt/ß-catenin signaling was inhibited by both CRNDE knockdown and miR-181a-5p overexpression. Significantly, we found that the repression of cell proliferation, the reduction of chemoresistance, and the inhibition of Wnt/ß-catenin signaling induced by CRNDE knockdown would require the increased expression of miR-181a-5p. CONCLUSIONS: Our study demonstrated that the lncRNA CRNDE could regulate the progression and chemoresistance of CRC via modulating the expression levels of miR-181a-5p and the activity of Wnt/ß-catenin signaling.


Assuntos
Resistencia a Medicamentos Antineoplásicos/genética , Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , RNA Longo não Codificante/genética , Via de Sinalização Wnt , Adulto , Idoso , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/genética , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Biológicos , Gradação de Tumores , Metástase Neoplásica , Estadiamento de Neoplasias , Interferência de RNA , Fator de Transcrição 4 , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Carga Tumoral , beta Catenina/genética , beta Catenina/metabolismo
15.
Cancer Lett ; 383(2): 195-203, 2016 12 28.
Artigo em Inglês | MEDLINE | ID: mdl-27721017

RESUMO

Ephrin Type-A Receptor 3 (EphA3) belongs to the ephrin receptor subfamily of the protein tyrosine kinase family, and plays an important role in embryogenesis and neurogenesis. This study aimed to investigate the role of EphA3 in promoting malignant transformation of colorectal epithelial cells, and explore underlying molecular mechanisms. Colorectal cancer tissue specimens from 68 patients were analyzed for EphA3 expression. EphA3 expression levels were manipulated in rat colon epithelial cell lines. We found that EphA3 expression level in tumor tissues was associated with patient age (P = 0.015), tumor differentiation (P = 0.001), and lymph node metastasis (P = 0.039). Overexpression of EphA3 and its constitutively active mutants promoted colony formation, migration and invasion, and tumorigenicity of colon epithelial cells in nude mice. The cDNA and lncRNA microarray profiling data revealed that differentially expressed genes and lncRNAs in EphA3 or mutant-transfected cells were associated with cell proliferation, invasion and angiogenesis. Our findings reveal the mechanisms underlying the oncogenic activities of EphA3 in colorectal cells, which could provide novel targets for the prevention, early diagnosis, and treatment of colorectal cancer.


Assuntos
Transformação Celular Neoplásica/metabolismo , Colo/metabolismo , Neoplasias Colorretais/metabolismo , Células Epiteliais/metabolismo , Oncogenes , Receptores Proteína Tirosina Quinases/metabolismo , Linhagem Celular , Movimento Celular , Proliferação de Células , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/patologia , Colo/patologia , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Biologia Computacional , Células Epiteliais/patologia , Feminino , Perfilação da Expressão Gênica/métodos , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Mutação , Neovascularização Patológica , Análise de Sequência com Séries de Oligonucleotídeos , Proteômica , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores Proteína Tirosina Quinases/genética , Transdução de Sinais , Fatores de Tempo , Transfecção
16.
Zhongguo Dang Dai Er Ke Za Zhi ; 18(5): 396-9, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-27165586

RESUMO

OBJECTIVE: To study the role of serum neutrophil elastase (NE) level in acute exacerbation of asthma in preschool children. METHODS: A total of 85 preschool children who were diagnosed with asthma between January 2008 and January 2010 were classified into acute exacerbation group (n=44) and non-acute exacerbation group (n=41). Thirty-five children who received physical examination served as the control group. The enzyme-linked immunosorbent assay was used to determine the serum levels of NE and interleukin-8 (IL-8). The receiver operating characteristic (ROC) curve was used for NE evaluation. RESULTS: Both the acute and non-acute exacerbation groups had higher serum levels of NE and IL-8 than the control group, and the acute exacerbation group had significantly higher serum levels of NE and IL-8 than the non-acute exacerbation group (P<0.05). The serum level of NE was positively correlated with that of IL-8 (r=0.48, P<0.05). With serum NE level >27.73 µg/L as the cut-off value for diagnosing acute exacerbation of asthma, the sensitivity was 65.9%, the specificity was 95.1%, and the area under the ROC curve was 0.87 (P<0.01). CONCLUSIONS: The determination of serum NE level in preschool children with asthma helps to diagnose the acute exacerbation of asthma.


Assuntos
Asma/diagnóstico , Elastase de Leucócito/sangue , Asma/sangue , Asma/complicações , Criança , Pré-Escolar , Feminino , Humanos , Interleucina-8/sangue , Masculino , Curva ROC
17.
Oncotarget ; 7(21): 31111-21, 2016 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-27145271

RESUMO

The erythropoietin-producing hepatocyte (Eph) family tyrosine kinases play important roles in tumorigenesis and cancer aggression. In this study, we investigated the role of EphB6 in oncogenic transformation of colorectal epithelial cells in vitro and in vivo. EphB6 is upregulated in human colorectal cancer (CRC) tissues as compared to normal tissues, and its overexpression promotes proliferation, migration and invasion by IMCE colorectal adenoma cells, in which one Apc allele is mutated. EphB6 overexpression together with Apc mutation leads to the development of colorectal tumors in vivo. Expression microarrays using mRNAs and lncRNAs isolated from EphB6-overexpresssing IMCE and control cells revealed a large number of dysregulated genes involved in cancer-related functions and pathways. The present study is the first to demonstrate that EphB6 overexpression together with Apc gene mutations may enhance proliferation, invasion and metastasis by colorectal epithelial cells. Microarray data and pathway analysis of differentially expressed genes provided insight into possible EphB6-regulated mechanisms promoting tumorigenesis and cancer progression. EphB6 overexpression may represent a novel, effective biomarker predictive of cell proliferation, invasion and metastasis patterns in CRC tumors.


Assuntos
Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Genes APC , Mutação , Receptores da Família Eph/biossíntese , Animais , Linhagem Celular Tumoral , Proliferação de Células , Neoplasias Colorretais/patologia , Regulação Neoplásica da Expressão Gênica , Xenoenxertos , Humanos , Camundongos , Camundongos Nus , Ratos , Receptores da Família Eph/genética , Transdução de Sinais , Transfecção
18.
Minerva Pediatr ; 68(1): 5-10, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25823620

RESUMO

BACKGROUND: This study aims to explore the correlation between nutrient level and pneumonia via the analysis and intervention of nutrient levels in pediatric patients with pneumonia. METHODS: Nutrient deficient children with pneumonia were randomized into intervention and non-intervention groups, and healthy children with the same age served as controls. Serum vitamin and trace element levels were determined. The nutrient levels, average hospital stay and nutrient deficiency rate were compared between groups. RESULTS: The pneumonia group showed significantly higher rates of iron, zinc and vitamin A deficiencies than the control group. The serum vitamin D level in asthmatic pneumonia group was lower than that in non-asthmatic pneumonia group and control group. Serum zinc, iron and vitamin A levels in the pneumonia group distinctly increased after intervention therapy. After vitamin D supplementation, the serum vitamin D level in asthmatic pneumonia group was significantly improved. Children in the intervention group had shorter hospital stays than children in the non-intervention group, whose hospital stays were longer than pediatric patients with normal nutrient levels. However, the difference between the intervention and normal nutrient groups was insignificant. CONCLUSION: Clinical nutrition intervention could improve the efficacy of pneumonia in pediatric patients and shorten hospital stay.


Assuntos
Estado Nutricional , Pneumonia/sangue , Oligoelementos/sangue , Vitaminas/sangue , Asma/sangue , Asma/complicações , Deficiência de Vitaminas/tratamento farmacológico , Deficiência de Vitaminas/epidemiologia , Pré-Escolar , Suplementos Nutricionais , Feminino , Hospitalização/estatística & dados numéricos , Humanos , Lactente , Tempo de Internação , Masculino , Pneumonia/tratamento farmacológico , Oligoelementos/administração & dosagem , Oligoelementos/deficiência , Resultado do Tratamento , Vitamina D/administração & dosagem , Vitamina D/sangue , Deficiência de Vitamina D/tratamento farmacológico , Deficiência de Vitamina D/epidemiologia , Vitaminas/administração & dosagem
19.
Zhongguo Dang Dai Er Ke Za Zhi ; 17(8): 815-8, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26287345

RESUMO

OBJECTIVE: To study whether infantile wheezing pneumonia has similar immune mechanisms to asthma by determining the levels of serum inflammatory factors in wheezing infants with community-acquired pneumonia (CAP). METHODS: Forty-two infants with CAP but without wheezing, 47 infants with CAP and wheezing, and 30 healthy infants as a control were recruited in the study. The peripheral blood levels of C-reactive protein, procalcitonin, soluble triggering receptor expressed on myeloid cell-l, interferon-γ, interleukin-4, interleukin-10, and periostin were compared in the three groups. RESULTS: The serum levels of procalcitonin, soluble triggering receptor expressed on myeloid cell-l, interleukin-4 and interleukin-10 in the two CAP groups were higher than in the control group (P<0.05). The ratio of interferon-γ/interleukin-4 in the wheezing pneumonia group was lower than in the non-wheezing pneumonia and control groups (P<0.05). The serum level of periostin in the wheezing pneumonia group was higher than in the non-wheezing pneumonia and control groups (P<0.05). CONCLUSIONS: The unbalanced ratio of interferon-γ/interleukin-4 and airway eosinophilic inflammation in wheezing infants with pneumonia suggest infantile pneumonia with wheezing may has similar immune mechanisms to asthma.


Assuntos
Infecções Comunitárias Adquiridas/imunologia , Pneumonia/imunologia , Sons Respiratórios/imunologia , Pré-Escolar , Feminino , Humanos , Lactente , Interferon gama/sangue , Interleucina-10/sangue , Interleucina-4/sangue , Masculino , Glicoproteínas de Membrana/sangue , Receptores Imunológicos/sangue , Receptor Gatilho 1 Expresso em Células Mieloides
20.
Plast Reconstr Surg ; 135(2): 331e-9e, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25626818

RESUMO

BACKGROUND: The use of vascularized nerve graft models has been limited because of the complexity of the operation. The authors sought to develop a simple and effective rabbit model for facial nerve repair and evaluated its advantages over conventional nerve grafts. METHODS: Rabbits were divided into three groups consisting of six rabbits each. The central auricular nerve and its nutrient vessels were used as a vascularized graft. Rabbits were grafted with a vascularized facial nerve graft (vascularized nerve graft group), with a free nerve graft (free nerve graft group), or with a vascularized nerve graft and a free nerve graft on each side of the face (vascularized nerve graft/free nerve graft group). Four months after surgery, facial performance and electrophysiologic monitoring were evaluated. The rabbits were then killed to prepare the nerve specimens for histologic, immunohistochemical, and transmission electron microscope study. RESULTS: At 4 months after the facial nerve repair, the functional recovery of the facial nerve was observed and analyzed. The side grafted with vascularized nerve graft was superior to the side grafted with free nerve graft. Regenerated nerve fibers were observed in all groups, and rabbits grafted with vascularized nerve grafts had more regenerated axons than those that underwent free nerve grafting, although the regenerated nerves were not as good as the natural nerves. CONCLUSIONS: This study demonstrates that it is feasible to establish a vascularized nerve graft model in rabbits. The model offers the obvious advantages of operability and reliability. The vascularized nerve graft is demonstrated to have a superior value for facial nerve repair.


Assuntos
Nervo Facial/cirurgia , Modelos Animais , Transferência de Nervo/métodos , Coelhos/cirurgia , Animais , Assimetria Facial/prevenção & controle , Nervo Facial/irrigação sanguínea , Nervo Facial/fisiologia , Nervo Facial/ultraestrutura , Microscopia Eletrônica , Regeneração Nervosa , Condução Nervosa , Complicações Pós-Operatórias/prevenção & controle , Distribuição Aleatória , Recuperação de Função Fisiológica , Células de Schwann/ultraestrutura
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA