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Mol Med Rep ; 23(1): 1, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33200797


MicroRNA (miR)­140­5p is associated with the growth and metastasis of various tumor cell types, yet its role in multiple myeloma (MM) remains unclear. Therefore, the present study aimed to investigate the regulatory effect of miR­140­5p on MM. Reverse transcription­quantitative PCR analysis demonstrated that miR­140­5p was downregulated in MM cell lines, particularly in U266 and RPMI 8226 cells. A Cell Counting Kit­8, wound healing and Transwell assays, as well as flow cytometry indicated that a miR­140­5p mimic could suppress cell viability, migration and invasion. In addition, the mimic promoted apoptosis of U266 and RPMI 8226 cells. Western blot data demonstrated that transfection with miR­140­5p mimic significantly reduced the expression levels of Ki­67, cyclin D1, vimentin, Snail, matrix metalloproteinase (MMP)­2 and MMP­3. Moreover, as predicted by TargetScan7.2 and verified by luciferase activity assay, it was demonstrated that vascular endothelial growth factor A (VEGFA) was targeted by miR­140­5p. Further experiments indicated that VEGFA overexpression promoted cell viability, migration and invasion and suppressed apoptosis of MM cells, and that the miR­140­5p mimic partially reversed the effects of VEGFA overexpression. Therefore, miR­140­5p suppressed MM progression by targeting VEGFA. The present findings provide insight into potential therapeutic strategies for the treatment of MM.

Onco Targets Ther ; 13: 309-321, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32021277


Background: Colorectal cancer (CRC) patients with different molecular phenotypes, including microsatellite instability (MSI), CpG island methylator phenotype (CIMP), and somatic mutations in BRAF and KRAS gene, vary in treatment response and prognosis. However, molecular phenotyping under adequate quality control in a community-based setting may be difficult. We aimed to build the nomograms based on easily accessible clinicopathological characteristics to predict molecular phenotypes. Methods: Three hundred and six patients with pathologically confirmed stage I-IV CRC were included in the cohort. The assays for MSI, CIMP, and mutations in BRAF and KRAS gene were performed using resected tumor samples. The candidate predictors were identified from clinicopathological variables using multivariate Logistic regression analyses to construct the nomograms that could predict each molecular phenotype. Results: The incidences of MSI, CIMP, BRAF mutation and KRAS mutation were 25.3% (72/285), 2.5% (7/270), 3.4% (10/293), and 34.8% (96/276) respectively. In the multivariate Logistic analysis, poor differentiation and high neutrophil/lymphocyte ratio (NLR) were independently associated with MSI; poor differentiation, high NLR and high carcinoembryonic antigen/tumor size ratio (CSR) were independently associated with CIMP; poor differentiation, lymphovascular invasion and high CSR were independently associated with BRAF mutation; poor differentiation, proximal tumor, mucinous tumor and high NLR were independently associated with KRAS mutation. Four nomograms for MSI, CIMP, BRAF mutation and KRAS mutation were developed based on these independent predictors, the C-indexes of which were 61.22% (95% CI: 60.28-62.16%), 95.57% (95% CI: 95.20-95.94%), 83.56% (95% CI: 81.54-85.58%), and 69.12% (95% CI: 68.30-69.94%) respectively. Conclusion: We established four nomograms using easily accessible variables that could well predict the presence of MSI, CIMP, BRAF mutation and KRAS mutation in CRC patients.

Huan Jing Ke Xue ; 40(6): 2533-2539, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854643


There are few analyses on the components of particulate matter emitted from waste incineration plants. In past studies, analyses of particle size distribution characteristics of the components were mainly targeted at particles with larger particle sizes. An electrical low pressure impactor (ELPI) was used in this study to collect the particulate matter emitted from a waste incineration plant, and the elements and carbonaceous components of these samples were analyzed. The particle size characteristics of organic carbon (OC), elemental carbon (EC), and heavy metal elements in 14 particle size segments were analyzed and composition profiles of elements and carbonaceous components of PM1, PM2.5, and PM10 from the waste incineration plant were established to provide a reference for refined source apportionment research. The results showed that the main components of the waste incineration plant included Al, Si, S, Ca, Cr, Fe, OC, EC, etc. OC and Ca were dominating components, and mass fractions of these components in the PM2.5 profile were 10.15% and 12.37%, respectively. The contents of heavy metals were ranked as Cr > Pb > Zn > Mn > Cu > Cd > Ni, and the mass fractions of Cr and Pb in PM2.5 amounted to 1.83% and 0.74%, respectively. OC in the range of 2.39-3.99 and 6.68-9.91 µm accounted for 15.02% and 20.45% of the total OC content, respectively, and the content of OC in fine particles was higher than that in coarse particles. The content of EC in fine particles was much higher than that in coarse particles, and it accounted for 14.8% in the 0.382-0.613 µm particle size. Heavy metal elements such as Cr, Mn, Ni, Cu, Zn, Cd, and Pb were mainly concentrated in the fine particles.