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1.
Int J Biol Macromol ; 130: 369-377, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30802516

RESUMO

The mitochondrial genome (mitogenome) provides important information for better understanding the phylogenetic relationships within heteropteran infraorder Cimicomorpha (Hemiptera: Heteroptera), but there are still limited representations at the family level of Anthocoridae. Here we sequenced the complete mitogenome of Tetraphleps aterrimus. It is 15,803 bp in size, and contains the expected 37 genes (13 PCGs, 22 tRNAs and 2 rRNAs) and control region. Gene order is identical to that of typical cimicomorphans. In comparison with other cimicomorphans, the ratios of Ka/Ks are increasing from 0.17 for COI to 0.85 for ATP8, which demonstrates COI shows the lowest evolutionary rate, while ATP8 appears to be the highest. The ratios of conserved sites of COI is the highest, while ATP8 is the lowest, suggesting that the evolutionary rate of ATP8 is higher than COI. The phylogenetic relationships based on mitogenomes using Bayesian inference (BI) and Maximum likelihood (ML) methods show that Tetraphleps aterrimus is sister to (Orius niger + Orius sauteri), suggesting that Tetraphleps aterrimus belongs to Anthocoridae. The monophyly of each superfamily is generally well supported and Reduvioidea is placed as basal branch in Cimicomorpha. The results support the remaining superfamily groupings (Miroidea + (Cimicoidea + (Velocipedoidea + Nabioidea))).


Assuntos
Genoma Mitocondrial/genética , Hemípteros/genética , Filogenia , Animais , Códon/genética , Evolução Molecular , Genômica , Proteínas de Insetos/genética , RNA Ribossômico/genética , RNA de Transferência/genética
2.
Mol Cell ; 72(2): 380-394.e7, 2018 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-30293782

RESUMO

RNA splicing is a critical mechanism by which to modify transcriptome, and its dysregulation is the underlying cause of many human diseases. It remains challenging, however, to genetically modulate a splicing event in its native context. Here, we demonstrate that a CRISPR-guided cytidine deaminase (i.e., targeted-AID mediated mutagenesis [TAM]) can efficiently modulate various forms of mRNA splicing. By converting invariant guanines to adenines at either 5' or 3' splice sites (SS), TAM induces exon skipping, activation of alternative SS, switching between mutually exclusive exons, or targeted intron retention. Conversely, TAM promotes downstream exon inclusion by mutating cytidines into thymines at the polypyrimidine tract. Applying this approach, we genetically restored the open reading frame and dystrophin function of a mutant DMD gene in patient-derived induced pluripotent stem cells (iPSCs). Thus, the CRISPR-guided cytidine deaminase provides a versatile genetic platform to modulate RNA splicing and to correct mutations associated with aberrant splicing in human diseases.


Assuntos
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética , Citidina Desaminase/genética , Processamento de RNA/genética , Sequência de Aminoácidos , Animais , Linhagem Celular , Distrofina/genética , Éxons/genética , Redes Reguladoras de Genes , Células HEK293 , Humanos , Íntrons/genética , Camundongos , Fases de Leitura Aberta/genética , Sítios de Splice de RNA/genética
3.
Int J Biol Macromol ; 119: 747-757, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30075212

RESUMO

The mitochondrial genome (mitogenome) has been extensively used to better understand the phylogenetic relationships within the heteropteran infraorder Nepomorpha (Hemiptera), but no mitogenome in Micronectidae has been sequenced to date. Here we describe the first complete mitogenome of Micronecta sahlbergii (Jakovlev, 1881). The mitogenome is 15,005 bp in size, containing 13 typical PCGs, 22 tRNAs, two rRNAs and a control region (CR). All genes are arranged in the same gene order as the most other known heteropteran mitogenome. The phylogenetic relationships based on mitogenomes using Bayesian inference and Maximum likelihood methods showed that Micronecta sahlbergii was sister to Sigara septemlineata, suggesting that Micronecta sahlbergii belongs to Corixoidea. Corixoidea was basal within Nepomorpha. The PCG12 and PCG12RT matrices of BI and ML analyses yielded the consistent topology, respectively. Whereas there was no consistent conclusions in PCG123 and PCG123RT matrices. Saturation tests showed that PCG12 and PCG12RT were free of saturation in evaluation of transition and transversion substitution, while PCG123 and PCG123RT exhibited a plateau revealing saturation of transition suggesting that the third codon positions of PCGs were not suitable for addressing relationships at the superfamily level in Nepomorpha. So our results supported the phylogenetic analysis of PCG12 and PCG12RT in Nepomorpha.


Assuntos
Genoma Mitocondrial , Hemípteros/classificação , Hemípteros/genética , Filogenia , Animais , Sequência de Bases , Códon , Genômica/métodos , Conformação de Ácido Nucleico , Fases de Leitura Aberta
4.
Nat Prod Res ; 32(12): 1451-1454, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28670931

RESUMO

This study represents the first report on the chemical composition and antimicrobial activity of the essential oil from the branches of Jacaranda cuspidifolia Mart. Thirty-three compounds were identified by Gas Chromatography-Mass Spectrometry (GC-MS) and the major constituents of the essential oil were Palmitic acid (31.36%), (Z) - 9,17-Octadecadienal (12.06%), Ethyl palmitate (3.81%), Perhydrofarnesyl acetone (2.07%), γ-Maaliene (1.88%), and Cedro (1.42%) and 9,12-Octadecadienoic acid ethyl ester (1.42%). The in vitro antimicrobial activities of the essential oil were evaluated by the disc diffusion method, and the inhibition zones against Escherichia coli, Staphylococcus aureus and Candida albicans were 7.10, 8.20 and 7.25 mm, respectively. The oil showed moderate activities against E. coli, S. aureus and C. albicans with minimum inhibition concentration (MIC) values of 17.3 mg/mL, 12.9 mg/mL and 16.0 mg/mL, respectively.


Assuntos
Anti-Infecciosos/farmacologia , Bignoniaceae/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Anti-Infecciosos/química , Candida albicans/efeitos dos fármacos , China , Escherichia coli/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Testes de Sensibilidade Microbiana , Staphylococcus aureus/efeitos dos fármacos
5.
Biomed Res Int ; 2018: 3429261, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30627549

RESUMO

Background: Recent several studies have showed that the nanog overexpression leads to poor prognosis in some kinds of cancer including hepatocellular carcinoma and gastrointestinal luminal cancer. However, the correlations between prognosis and clinic-pathological features and nanog overexpression in lung cancer are still not well-known. Thus, we performed a meta-analysis to evaluate the role of nanog in lung cancer. Methods: An electronic retrieval for related studies was conducted in PubMed, Cochrane Library, Web of Science, EMBASE databases, Chinese CNKI, and the Chinese Wan Fang database up to May 2018. The relationships between nanog overexpression and overall survival (OS) and disease-free survival (DFS) as well as clinic-pathological features in lung cancer were investigated. Pooled hazard ratios (HRs) and odds ratios (ORs) with 95% confidence intervals (CIs) were calculated by STATA12. Results: 11 studies containing 1422 patients were identified in our meta-analysis. The overexpression of nanog showed decreased OS (HR = 1.83, 95% CI = 1.49-2.25, P ≤ 0.001) and DFS (HR = 1.86, 95% CI = 1.2-2.9, P = 0.006). Moreover, overexpression of nanog was significantly related to differentiation (OR = 4.17, 95% CI = 2.17-6.43, P ≤ 0.001), lymph node metastasis (OR = 1.76, 95% CI = 1.06-2.91, P = 0.028) and tumor size (OR = 1.93, 95% CI = 1.17-3.20, P = 0.010), and no correlation with T stage, TNM, stage, and gender. Conclusions: Our results suggested that nanog overexpression, a hazard factor of differentiation, lymph node metastasis, and tumor size, may predicate decreased OS and DFS for lung cancer.


Assuntos
Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/mortalidade , Proteína Homeobox Nanog/biossíntese , Proteínas de Neoplasias/biossíntese , Intervalo Livre de Doença , Humanos , Neoplasias Pulmonares/patologia , Metástase Linfática , Taxa de Sobrevida
6.
Pharmacogn Mag ; 13(51): 488-491, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28839377

RESUMO

BACKGROUND: The genus Wikstroemia has about 70 species, but only a limited number of species have been studied chemically. Wikstroemia indica has long been used as a traditional crude drug in China. However, there is no report about the bioactivity of Wikstroemia scytophylla. OBJECTIVE: This paper reports the chemical investigation and biological evaluation of the W. scytophylla. MATERIALS AND METHODS: The EtOAc extraction of W. scytophylla was isolated using chromatographic methods, and the compounds were analyzed by spectroscopic methods. The in vitro antitumor activities against five human cancer cell lines were performed according to the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. RESULTS: The chemical investigation of the stems of W. scytophylla resulted in the isolation of 12 compounds mainly including one biflavone (1), five flavones (2-6) compounds, and six lignans (7-12), in which compound 8 was a new natural product. Compounds 1 and 7-12 were evaluated for their antitumor activities while these compounds showed weak cytotoxicity with the half maximal inhibitory (IC50) values more than 40 µM. CONCLUSION: All of these compounds were isolated from this plant for the first time, and compounds 2-12 were first reported from genus Wikstroemia, in which compound 8 was a new natural product. Compounds 1 and 7-12 exhibited weak antitumor activities (IC50>40 µM). The chemotaxonomic significance of all the isolations was summarized. SUMMARY: The chemical investigation of the stems of W. scytophylla resulted in the isolation of 12 compoundsThe 12 compounds including six lignans (7-12), in which compound 8 was a new natural productThe isolated compounds 1 and 7-12 were evaluated for their antitumor activitiesThe chemotaxonomic significance of all the isolations was summarized. Abbreviations used: MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; IC50: Half maximal inhibitory; HL-60: Human leukemia cell line; SMMC-7721: Human hepatocellular carcinoma cell line; A549: Human lung tumor cell line; MCF-7: Human breast cancer cell line; SW480: Human colon cancer cell line; MS: Mass spectrometry; NMR: Nuclear Magnetic Resonance.

7.
J Biol Chem ; 292(29): 12285-12295, 2017 07 21.
Artigo em Inglês | MEDLINE | ID: mdl-28611064

RESUMO

Increasing evidence indicates that alternative processing of mRNA, including alternative splicing, 3' alternative polyadenylation, and regulation of mRNA stability/translation, represents a major mechanism contributing to protein diversification. For example, in alternative polyadenylation, the 3' end of the immunoglobulin heavy chain mRNA is processed during B cell differentiation, and this processing involves RNA-binding proteins. hnRNPLL (heterogeneous nuclear ribonucleoprotein L-like protein) is an RNA-binding protein expressed in terminally differentiated lymphocytes, such as memory T cells and plasma cells. hnRNPLL regulates various processes of RNA metabolism, including alternative pre-mRNA splicing and RNA stability. In plasma cells, hnRNPLL also regulates the transition from the membrane isoform of the immunoglobulin heavy-chain (mIgH) to the secreted isoform (sIgH), but the precise mechanism remains to be identified. In this study, we report that hnRNPLL specifically associates with cytoplasmic PABPC1 (poly(A)-binding protein 1) in both T cells and plasma cells. We found that although PABPC1 is not required for the alternative splicing of CD45, a primary target of hnRNPLL in lymphocytes, PABPC1 does promote the binding of hnRNPLL to the immunoglobulin mRNA and regulates switching from mIgH to sIgH in plasma cells. Given the recently identified role of PABPC1 in mRNA alternative polyadenylation, our findings suggest that PABPC1 recruits hnRNPLL to the 3'-end of RNA and regulates the transition from membrane Ig to secreted Ig through mRNA alternative polyadenylation. In conclusion, our study has revealed a mechanism that regulates immunoglobulin secretion in B cells via cooperation between a plasma cell-specific RBP (hnRNPLL) and a universally expressed RBP (PABPC1).


Assuntos
Citoplasma/metabolismo , Ribonucleoproteínas Nucleares Heterogêneas/metabolismo , Cadeias Pesadas de Imunoglobulinas/metabolismo , Plasmócitos/metabolismo , Proteína I de Ligação a Poli(A)/metabolismo , Poliadenilação , RNA Mensageiro/metabolismo , Animais , Células Cultivadas , Ribonucleoproteínas Nucleares Heterogêneas/química , Ribonucleoproteínas Nucleares Heterogêneas/genética , Humanos , Switching de Imunoglobulina , Cadeias Pesadas de Imunoglobulinas/genética , Imunoprecipitação , Células Jurkat , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos C57BL , Plasmócitos/citologia , Plasmócitos/imunologia , Proteína I de Ligação a Poli(A)/antagonistas & inibidores , Proteína I de Ligação a Poli(A)/química , Proteína I de Ligação a Poli(A)/genética , Domínios e Motivos de Interação entre Proteínas , Interferência de RNA , Baço/citologia , Baço/imunologia , Linfócitos T/citologia , Linfócitos T/imunologia , Linfócitos T/metabolismo
8.
Nat Prod Res ; 31(8): 896-901, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27820967

RESUMO

The present study was designed to investigate the chemical constituents of Lindera nacusua and their antitumor activities. A new phenolic glycoside, namely 1-O-3-hydroxyphenyl-5-methoxyphenol-(6'-O-vanilloyl)-ß-d-glucopyranoside (1), together with five known phenolic glycosides (2-6), two anthraquinones (7, 8) and two γ-butanolides (9, 10), was isolated, and its structure was elucidated by spectroscopic and chemical methods. Compounds 1-10 were screened for their in vitro cytotoxicities against HL-60, SMMC-7721, A549, MCF-3 and SW480 cell lines by the MTS method. Compounds 9 and 10 showed moderate cytotoxicities with IC50 values ranging from 17.40 to 35.21 µM.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Glicosídeos/química , Lindera/química , Monossacarídeos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Células HL-60 , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Monossacarídeos/química , Monossacarídeos/farmacologia , Fenóis/química , Fenóis/farmacologia , Caules de Planta/química
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