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1.
Sensors (Basel) ; 19(21)2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31671547

RESUMO

The deep convolutional neural network has led the trend of vision-based road detection, however, obtaining a full road area despite the occlusion from monocular vision remains challenging due to the dynamic scenes in autonomous driving. Inferring the occluded road area requires a comprehensive understanding of the geometry and the semantics of the visible scene. To this end, we create a small but effective dataset based on the KITTI dataset named KITTI-OFRS (KITTI-occlusion-free road segmentation) dataset and propose a lightweight and efficient, fully convolutional neural network called OFRSNet (occlusion-free road segmentation network) that learns to predict occluded portions of the road in the semantic domain by looking around foreground objects and visible road layout. In particular, the global context module is used to build up the down-sampling and joint context up-sampling block in our network, which promotes the performance of the network. Moreover, a spatially-weighted cross-entropy loss is designed to significantly increases the accuracy of this task. Extensive experiments on different datasets verify the effectiveness of the proposed approach, and comparisons with current excellent methods show that the proposed method outperforms the baseline models by obtaining a better trade-off between accuracy and runtime, which makes our approach is able to be applied to autonomous vehicles in real-time.

2.
J Biophotonics ; : e201960075, 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31593614

RESUMO

Intravital imaging has emerged as a novel and efficient tool for visualization of in situ dynamics of cellular behaviors and cell-microenvironment interactions in live animals, based on desirable microscopy techniques featuring high resolutions, deep imaging and low phototoxicity. Intravital imaging, especially based on multi-photon microscopy, has been used in bone research for dynamics visualization of a variety of physiological and pathological events at the cellular level, such as bone remodeling, hematopoiesis, immune responses and cancer development, thus, providing guidance for elucidating novel cellular mechanisms in bone biology as well as guidance for new therapies. This review is aimed at interpreting development and advantages of intravital imaging in bone research, and related representative discoveries concerning bone matrices, vessels, and various cells types involved in bone physiologies and pathologies. Finally, current limitations, further refinement, and extended application of intravital imaging in bone research are concluded.

3.
J Vis Exp ; (151)2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31609313

RESUMO

Highly motile dendritic filopodia are widely present in neurons at early developmental stages. These exploratory dynamic branches sample the surrounding environment and initiate contacts with potential synaptic partners. Although the connection between dendritic branch dynamics and synaptogenesis is well established, how developmental and activity-dependent processes regulate dendritic branch dynamics is not well understood. This is partly due to the technical difficulties associated with the live imaging and quantitative analyses of these fine structures using an in vivo system. We established a method to study dendrite dynamics using Drosophila larval ventral lateral neurons (LNvs), which can be individually labeled using genetic approaches and are accessible for live imaging. Taking advantage of this system, we developed protocols to capture branch dynamics of the whole dendritic arbor of a single labeled LNv through time-lapse live imaging. We then performed post-processing to improve image quality through drift correction and deconvolution, followed by analyzing branch dynamics at the single-branch level by annotating spatial positions of all branch terminals. Lastly, we developed R scripts (Supplementary File) and specific parameters to quantify branch dynamics using the coordinate information generated by the terminal tracing. Collectively, this protocol allows us to achieve a detailed quantitative description of branch dynamics of the neuronal dendritic arbor with high temporal and spatial resolution. The methods we developed are generally applicable to sparsely labeled neurons in both in vitro and in vivo conditions.

4.
Plast Reconstr Surg ; 144(4): 869-880, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31568294

RESUMO

BACKGROUND: The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis that are closely related to unsatisfactory vascularization. Extracellular vesicles are key components of the cell secretome, which can mirror the functional and molecular characteristics of their parental cells. Growing evidence has revealed that adipose-derived mesenchymal stem cells have the ability to enhance vascularization, which is partly ascribed to extracellular vesicles. The authors evaluated whether adipose-derived mesenchymal stem cell-derived extracellular vesicles improved vascularization of fat grafts and increased their retention rate. METHODS: To test the angiogenesis ability of adipose-derived mesenchymal stem cell-derived extracellular vesicles, they were isolated from the supernatant of cultured human adipose-derived mesenchymal stem cells and incubated with human umbilical vein endothelial cells in vitro. Then, the vesicles were co-transplanted with fat into nude mice subcutaneously. Three months after transplantation, the retention rate and inflammatory reaction of the grafts were analyzed by histologic assay. RESULTS: The experimental group could significantly promote migration and tube formation at the concentration of 20 µg/ml. At 3 months after transplantation, the volume of the experimental group (0.12 ± 0.03 mm) was larger compared with the blank group (0.05 ± 0.01 mm). Histology and immunohistology results demonstrated significantly fewer cysts and vacuoles, less fibrosis, and more neovessels in the extracelluar vesicle group. CONCLUSIONS: The authors co-transplanted adipose-derived mesenchymal stem cell-derived extracellular vesicles with fat into a nude mouse model and found that the vesicles improved volume retention by enhancing vascularization and regulating the inflammatory response.

5.
Accid Anal Prev ; 134: 105324, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31648116

RESUMO

OBJECTIVE: Side crashes between vehicles which usually lead to high casualties and property loss, rank first among total crashes in China. This paper aims to identify the factors associated with injury severity of side crashes at intersections and to provide suggestions for developing countermeasures to mitigate the levels of injuries. METHOD: In order to investigate the role of striking and struck vehicles in side crashes simultaneously, bivariate probit model was proposed and Bayesian approach was employed to evaluate the model, compared to the corresponding univariate probit model. DATA: Crash data from Beijing, China for the period 2009-2012 were used to carry out the statistical analysis. Based on the investigation with vehicles and data analysis on events, 130 intersection side crash cases were selected to form a specific dataset. Then, the influence of human, vehicles, roadway and environmental variables on crash severity was examined by means of bivariate probit regression within Bayesian framework. RESULTS: The effects of the factors on striking vehicle drivers and struck vehicle drivers were considered separately and simultaneously to find more targeted conclusions. The statistical analysis revealed vehicle type, lane number, no non-motorized lane and speeding have the corresponding influence on the injury severity of striking vehicles, while time of day and vehicle type of struck vehicles increased the likelihood of being injured. CONCLUSIONS: From the results it can be concluded that there indeed exists correlation between striking and struck vehicles in side crashes, although the correlation is not so strong. Importantly, Bayesian bivariate probit model can address the role of striking and struck vehicles in side crashes simultaneously and can accommodate the correlation clearly, which extends the range of univariate probit analysis. The general and empirical countermeasures are presented to improve the safety at intersections.

6.
Nanomedicine (Lond) ; 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31603382

RESUMO

Aim: Traditional antigenicity assay requires antigen recovery from the particulate adjuvants prior to analysis. An in situ method was developed for interrogating vaccine antigens with monoclonal antibodies while being adsorbed on adjuvants. Materials & methods: The fluorescence imaging-based high content analysis was used to visualize the antigen distribution on adjuvant agglomerates and to analyze the antigenicity for adsorbed antigens. Results: Simultaneous visualization and quantitation were achieved for dual antigens in a bivalent human papillomavirus vaccine with uniquely labeled antibodies. Good agreement was observed between the in situ multiplexed assays with well-established sandwich enzyme-linked immunosorbent assays. Conclusion: The streamlined procedures and the amenability for multiplexing make the in situ antigenicity analysis a favorable choice for in vitro functional assessment of bionanoparticles as vaccine antigens.

7.
Biofabrication ; 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31665714

RESUMO

Breast tissue engineering is a promising alternative to standard treatments for breast defect. Although there is a consensus that the mechanical property of the scaffold should best match the reconstructed tissue, the simulation of soft and elastic tactility of native breast tissues using conventional materials and architecture design requires further study. Previous researches have shown that the crystal microstructure-like design can drastically alter the mechanical properties of the constructed scaffolds. In this study, we designed and additive manufactured four kinds of breast scaffolds using polyurethane and termed their architectures as N5S4, N9S8, N7S6, N4S6. The basic unit cell of each scaffold was similar to a lattice structure from the isometric crystal system. The scaffolds possessed identical porosities but different mechanical properties in which the compressive modulus of the softest scaffolds (N5S4) were similar to that of native breast tissue. When applied in the construction of tissue engineered breast combining with delayed fat injection technique in nude rat models, the soft scaffolds(N5S4) performed better compared to its stiff counterparts(N4S6) as in N5S4 scaffolds higher adipose survival, vascularization and milder fibrosis could be observed. Lastly, using finite element analysis, we further investigated the influence of the unit cell architectures on the mechanical properties of the scaffolds and detailly simulated the deformation as well as stress distribution patterns of the implanted scaffolds. A crystal lattice-like architecture design was introduced to tune the mechanical properties of the scaffolds and match the requirements for tissue engineering applications.

8.
Theranostics ; 9(20): 5839-5853, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31534523

RESUMO

Wound dressings composed of natural polymers, such as type I collagen, possess good biocompatibility, water holding capacity, air permeability, and degradability, and can be used in wound repair. However, due to the persistent oxidative stress in the wound area, the migration and proliferation of fibroblasts might be suppressed, leading to poor healing. Thus, collagen-containing scaffolds are not suitable for accelerated wound healing. Antioxidant N-acetyl cysteine (NAC) is known to reduce the reactive oxygen species (ROS) and has been widely used in the clinic. Theoretically, the carboxyl group of NAC allows loading of graphene oxide (GO) for sustained release and may also enhance the mechanical properties of the collagen scaffold, making it a better wound-dressing material. Herein, we demonstrated an innovative approach for a potential skin-regenerating hybrid membrane using GO incorporated with collagen I and NAC (N-Col-GO) capable of continuously releasing antioxidant NAC. Methods: The mechanical stability, water holding capacity, and biocompatibility of the N-Col-GO hybrid membrane were measured in vitro. A 20 mm rat full-skin defect model was created to evaluate the repair efficiency of the N-Col-GO hybrid membrane. The vascularization and scar-related genes in the wound area were also examined. Results: Compared to the Col only scaffold, N-Col-GO hybrid membrane exhibited a better mechanical property, stronger water retention capacity, and slower NAC release ability, which likely promote fibroblast migration and proliferation. Treatment with the N-Col-GO hybrid membrane in the rat wound model showed complete healing 14 days after application which was 22% faster than the control group. HE and Masson staining confirmed faster collagen deposition and better epithelization, while CD31 staining revealed a noticeable increase of vascularization. Furthermore, Rt-PCR demonstrated decreased mRNA expression of profibrotic and overexpression of anti-fibrotic factors indicative of the anti-scar effect. Conclusion: These findings suggest that N-Col-GO drug release hybrid membrane serves as a better platform for scarless skin regeneration.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31487886

RESUMO

The filling ratio (FR) of a carrier has an influence on the pollutant removal of the aerobic moving bed biofilm reactor (MBBR). However, the effect of the polyethylene (PE) carrier FR on the performance and microbial characteristics of the denitrifying MBBR for the treatment of wastewater treatment plant (WWTP) effluent has not been extensively studied. A bench-scale denitrifying MBBR was set up and operated with PE carrier FRs of 20%, 30%, 40%, and 50% for the degradation of chemical oxygen demand (COD) and nitrogen from WWTP effluent at 12 h hydraulic retention time (HRT). The nitrate removal rates with FRs of 20%, 30%, 40%, and 50% were 94.3 ± 3.9%, 87.7 ± 7.3%, 89.7 ± 11.6%, and 94.6 ± 4.0%, and the corresponding denitrification rates (rNO3--N) were 8.0 ± 5.6, 11.3 ± 4.6, 11.6 ± 4.6, and 10.0 ± 4.9 mg NO3--N/L/d, respectively. Nitrous oxide reductase (nosZ) gene-based terminal restriction fragment length polymorphism (T-RFLP) analysis illustrated that the highest functional diversity (Shannon's diversity index, H') of biofilm microbial community was obtained at 30% FR. The quantitative polymerase chain reaction (qPCR) results indicated that the abundance of nitrate reductase (narG) and nosZ genes at 30% FR was significantly higher than that at 20% FR, and no significant changes were observed at 40% and 50% FRs. Thus, 30% FR was recommended as the optimal carrier FR for the denitrifying MBBR.

10.
Traffic Inj Prev ; 20(7): 764-770, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31486678

RESUMO

Objective: E-commerce has increased freight volumes and shifted freight vehicles to local streets and arterials. These changes have caused increasing concern over traffic safety in urban areas, but there has been limited investigation of road safety impacts. To advance understanding of this issue, we examined recent trends in urban freight-related safety in the United States and characteristics of these crashes. Methods: We estimated annual rates of fatal and nonfatal urban freight-related injuries from 2005 through 2015 using data from the NHTSA's Fatal Analysis Reporting System and National Automotive Sampling System General Estimates System. We compared trends in fatal and nonfatal urban freight-related injuries, stratified by road type (interstate vs. not), to overall crash injury trends. We used piecewise linear regression models to test trend changes. We examined and compared specific crash and injury characteristics associated with urban freight-involved crashes, compared to other types of crashes, for the most recent year of data analyzed. Results: Though freight and overall crash fatality rates per vehicle mile traveled declined between 2005 and 2009, increases were observed between 2009 and 2015. Fatality rates increased 3% for all vehicle crashes, 17% for urban freight-involved crashes, and 15% for urban freight-involved crashes not occurring on interstates. Nonfatal injury rates for urban freight-involved crashes rose even more rapidly between 2009 and 2015. Nonfatal injury rates for urban freight-involved crashes increased 45%, rates for urban freight-involved crashes not occurring on interstates increased 40%, and overall nonfatal injury rates for all vehicle crashes increased 3% during this time. Of all urban freight-related crashes resulting in nonfatal injury, the proportion occurring on non-interstate roads increased from 17% to 25% between 2005 and 2015, and these crashes were more likely to occur on weekdays between 6 a.m. and 4 p.m., when freight demand is high, than weekends or evenings. Conclusions: Freight-involved injury and fatality rates are rising more rapidly than overall road traffic-related rates, both in all areas and in urban areas. These crashes are also increasingly occurring on local roads and arterials as opposed to interstates. These findings can help policymakers better understand the changing patterns of freight-related safety issues. As freight volumes increase in commercial and residential areas, planners must increasingly consider freight needs and ensure that space is allocated to this function. Additionally, changes to surveillance systems are necessary to better track burden and risk factors associated with these crashes and inform crash prevention efforts.

11.
Nat Commun ; 10(1): 4093, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31501438

RESUMO

ON and OFF selectivity in visual processing is encoded by parallel pathways that respond to either light increments or decrements. Despite lacking the anatomical features to support split channels, Drosophila larvae effectively perform visually-guided behaviors. To understand principles guiding visual computation in this simple circuit, we focus on investigating the physiological properties and behavioral relevance of larval visual interneurons. We find that the ON vs. OFF discrimination in the larval visual circuit emerges through light-elicited cholinergic signaling that depolarizes a cholinergic interneuron (cha-lOLP) and hyperpolarizes a glutamatergic interneuron (glu-lOLP). Genetic studies further indicate that muscarinic acetylcholine receptor (mAchR)/Gαo signaling produces the sign-inversion required for OFF detection in glu-lOLP, the disruption of which strongly impacts both physiological responses of downstream projection neurons and dark-induced pausing behavior. Together, our studies identify the molecular and circuit mechanisms underlying ON vs. OFF discrimination in the Drosophila larval visual system.

12.
Appl Opt ; 58(25): 6841-6847, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31503654

RESUMO

Dissipative soliton resonance (DSR) with multiple stable states has been experimentally demonstrated in a passively mode-locked Yb-doped fiber laser. The generation of the DSR square pulse has been achieved with the nonlinear optical loop mirror technique. The duration of the square pulse varies from 16 to 123 ns, while the amplitude of the pulse remains constant. Experimentally, by fixing the pump power and changing the orientations of the polarization controllers, we have observed three different square pulses that all operate in the DSR regime. At the same time, we have obtained a wavelength-tunable square pulse, which demonstrates that the DSR and operation wavelength are independent of each other. We also analyzed the ideal state in the DSR regime.

13.
Appl Opt ; 58(23): 6464-6469, 2019 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-31503796

RESUMO

We report on the experimental generation of various self-organized structures of bound states in a near zero-dispersion mode-locked fiber laser. When the pump power is fixed at 492 mW, appropriately adjusting polarization controllers, the switching of the cavity feedback results in the evolution from the single pulse to the dispersion-managed soliton (i.e., stretched-pulse) pair. With the increase of pump power, bound states composed of more than two pulses can also be observed. Our results of the self-organized structures might enlarge the data-carrying capacity of current fiber-optical communication systems and benefit the investigation of nonlinear dynamics of bound states in fiber lasers at 2 µm.

14.
Appl Opt ; 58(18): 4956-4962, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31503817

RESUMO

We report on the experimental observation of wavelength-switchable stretched pulse and bound-state pulse in a dispersion-managed Tm-doped laser. At a pump power of 572 mW, a stretched pulse with a pulse duration of 389 fs can be first obtained at 1961 nm. By increasing the pump power and appropriately adjusting the cavity polarization state, the mode-locking wavelength can be switched from 1961 nm to 1980 nm caused by the birefringence filtering effect based on nonlinear polarization rotation, and the corresponding pulse duration is 371 fs. Meanwhile, loosely bound states of two pulses and three pules at 1980 nm can be observed with appropriate cavity parameters.

15.
J Cell Biochem ; 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31385362

RESUMO

Glioblastoma multiforme (GBM) is a refractory tumor with poor prognosis and requires more effective treatment regimens. It has been confirmed that long noncoding RNAs (lncRNAs) substantially regulate various human disease including GBM. However, the biological roles and its underlying molecular mechanisms still need to be further investigated. In this study, the biological function and potential molecular mechanism of lncHAS2-AS1 in GBM were explored. It was discovered that HAS2-AS1 was elevated in glioma tissues and correlated with the prognosis of patients with glioma. Reduction of HAS2-AS1 suppressed the migration and invasion in vitro and in vivo. The transcription factor STAT1 could raise HAS2-AS1 by binding to its promoter region. Besides, HAS2-AS1 could adjust PRPS1 via sponging miR-608 in a direct manner. On the whole, the results of this study evidence that HAS2-AS1 is an oncogene and a potential therapeutic target for GBM.

16.
Cell Prolif ; : e12680, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31454111

RESUMO

OBJECTIVES: The odontoblastic differentiation of dental pulp stem cells (DPSCs) contributes to tertiary dentin formation. Our previous study indicated that epiregulin (EREG) enhanced odontogenesis potential of dental pulp. Here, we explored the effects of EREG during DPSC odontoblastic differentiation. METHODS: The changes in EREG were detected during tertiary dentin formation. DPSCs were treated with recombinant human EREG (rhEREG), EREG receptor inhibitor gefitinib and short hairpin RNAs. The odontoblastic differentiation was assessed with ALP staining, ALP activity assay, alizarin red S staining and real-time RT-PCR of DSPP, OCN, RUNX2 and OSX. Western blot was conducted to examine the levels of p38 mitogen-activated protein kinase (p38 MAPK), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase 1/2 (Erk1/2). The expression of EREG and odontoblastic differentiation-related markers was investigated in human dental pulp from teeth with deep caries and healthy teeth. RESULTS: Epiregulin was upregulated during tertiary dentin formation. rhEREG enhanced the odontoblastic differentiation of DPSCs following upregulated p38 MAPK and Erk1/2 phosphorylation, but not JNK, whereas depletion of EREG suppressed DPSC differentiation. Gefitinib decreased odontoblastic differentiation with decreased phosphorylation of p38 MAPK and Erk1/2. And suppression of p38 MAPK and Erk1/2 pathways attenuated DPSC differentiation. In human dental pulp tissue, EREG upregulation in deep caries correlates with odontoblastic differentiation enhancement. CONCLUSION: Epiregulin is released during tertiary dentin formation. And EREG enhanced DPSC odontoblastic differentiation via MAPK pathways.

17.
Methods Mol Biol ; 2046: 89-94, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31407298

RESUMO

In many anaerobic environments methane (CH4) is produced by methanogens, with either H2/CO2 or acetate (i.e., the methyl group) as precursors, through what are referred to as hydrogenotrophic and acetoclastic methanogenic pathways respectively. Their relative contribution to total CH4 production can be quantified by determining the stable carbon isotopic fractionation factors for both pathways as well as the isotopic signatures of CO2, CH4, and the methyl group in acetate of the sample. The procedures for measuring carbon isotopic fractionation factors of both methanogenic pathways and isotopic composition of these compounds by isotope ratio mass spectrometry are described in this chapter. The results are very helpful in evaluating the activity of the methanogens involved in each methanogenic pathway as well as those of other biological pathways with different fractionation factors.

18.
Plast Reconstr Surg ; 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31299680

RESUMO

BACKGROUND: The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis that are closely related to unsatisfactory vascularization. Extracellular vesicles (EVs) are key components of the cell secretome, which can mirror the functional and molecular characteristics of their parental cells. Growing evidence has revealed that adipose-derived mesenchymal stem cells (ADSCs) have the ability to enhance vascularization which is partly ascribed to EVs. We evaluated whether ADSC-derived extracellular vesicles (ADSC-EVs) improved vascularization of fat grafts and increased their retention rate. METHODS: To test the angiogenesis ability of ADSC-EVs, they were isolated from the supernatant of cultured human ADSCs and incubated with human umbilical vein endothelial cells (HUVECs) in vitro. Then, ADSC-EVs were co-transplanted with fat into nude mice subcutaneously. After 3 months of transplantation, retention rate and inflammatory reaction of the grafts were analyzed by histology assay. RESULTS: ADSC-EVs could significantly promote migration and tube formation of HUVECs at the concentration of 20 µg/mL. At 3 month after transplantation, the volume of fat grafts supplemented with ADSC-EVs (0.12±0.03 mm) was larger compared with the Blank group (0.05±0.01 mm). Histological analysis demonstrated that transplants in the EV group had significantly less cysts, vacuoles, and fibrosis. Immunohistology assay further confirmed that ADSC-EVs could also promote vascularization (CD31-positive vessels: 9.8±1.7/field vs 20.0±4.9/field, P<0.05) and regulate inflammatory response (CD206-positive cells: 16.8±5.2/field vs 26.2±5.5/field, P<0.05). CONCLUSIONS: In this study, we co-transplanted ADSC-EVs with fat into nude mouse model and found that ADSC-EVs improved volume retention by enhancing vascularization and regulating the inflammatory response.

19.
Sheng Wu Gong Cheng Xue Bao ; 35(7): 1162-1173, 2019 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-31328473

RESUMO

Cell-penetrating peptides (CPPs) are short peptides that can penetrate the cell membrane or tissue barrier. CPPs can deliver a variety of biomacromolecules, such as proteins, RNA and DNA, into cells to produce intracellular functional effects. Endocytosis and direct penetration have been suggested as the two major uptake mechanisms for CPPs-mediated cargo delivery. Compared with other non-natural chemical molecules-based delivery reagents, the CPPs have better biocompatibility, lower cytotoxicity, are easily degraded after cargo delivery, and can be fused and recombined expressed with bioactive proteins. Because of these advantages, the CPPs have become an important potential tool for delivery of developing drugs which targets intracellular factors. As a novel delivery tool, the CPPs also show promising application prospects in biomedical researches. This review summarized recent advances regarding the classification characteristics, the cellular uptake mechanisms and therapeutic application potentials of CPPs.


Assuntos
Peptídeos Penetradores de Células/metabolismo , Transporte Biológico , Membrana Celular , Endocitose
20.
ACS Sens ; 4(8): 2124-2130, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31313911

RESUMO

Detection of biomarkers in complex samples is a significant health plan strategy for medical diagnosis, therapy monitoring, and health management. However, high background noise resulting from impurities and other analytes in complex samples has hampered the improvement of detection sensitivity and accuracy. Herein, an ultralow background biochip based on time-gated luminescent probes supported by photonic crystals (PCs) was successfully developed for detection of bladder cancer (BC)-related miRNA biomarkers with high sensitivity and specificity in urine samples. Coupled with the time-gated luminescence of long-lifetime luminescence probes and the luminescence-enhanced capability of PCs, the short-lived autofluorescence can be efficiently removed; thus, the detection sensitivity will be significantly improved. Benefiting from these merits, a detection limit of 26.3 fM is achieved. Furthermore, the biochip exhibits excellent performance in urinary miRNA detection, and good recoveries are also obtained. The developed biochip possesses unique properties of ultralow background and luminescence enhancement, thus offering a suitable tool for the detection of BC-related miRNA in urine. With rational design of probe sequences, the biochip holds great potential for many other biomarkers in real patient samples, making it valuable in areas such as medical diagnosis and disease evaluation.

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