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1.
Anat Rec (Hoboken) ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31692261

RESUMO

Recent studies have reported the anticancer activity of huaier extract in various human malignancies. However, little is known about the effect of huaier extract in non-small cell lung cancer (NSCLC) and its underlying mechanism. The current study aimed to investigate whether huaier extract affects the progression of NSCLC. mRNA and proteins expression of pyroptotic-related genes (NLRP3, caspase-1, IL-1ß, and IL-18) in NSCLC tissues and cells were, respectively, detected by qRT-PCR and western blot. The effects of huaier extract on NSCLC cell viability and cytotoxicity were evaluated by CCK-8 assay, colony formation assay, and LDH detection kit. Besides, we established a xenograft model to assess the antitumor effect of huaier extract on tumor growth in vivo. Our results showed that the expression of pyroptotic-related genes was downregulated in NSCLC tissues and cell lines. Huaier extract pretreatment inhibited cell viability and the percentage of colony formation of H520 and H358 cells, and upregulated the expression of pyroptotic-related genes. Mechanistically, huaier extract exhibited antitumor effect in NSCLC via inducing NLRP3-dependent pyroptosis in vitro and in vivo. In conclusion, our finding confirmed that huaier extract played an antitumor role in NSCLC progression through promoting pyroptotic cell death, which provided a new potential strategy for NSCLC clinical treatment.

2.
Opt Express ; 27(18): 25994-26013, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31510461

RESUMO

The classic Czerny-Turner spectrometer consists of a plane grating and two spherical mirrors. The optical path geometry adopted for incident and grating dispersed light is off-axis reflection, so the spherical collimating and focusing mirrors introduce coma and astigmatism. The conventional configuration is asymmetrical for coma automatic compensation, but suffers from astigmatism. We substitute the off-axis parabolic (OAP) surfaces for spherical surfaces of the collimating mirror and each sub-region of the focusing mirror, to achieve an aberration free configuration. The multiple OAP surfaces are then expanded and mixed, to construct a freeform surface integrating the collimating and focusing mirrors into a single element. Results show that a 0.1 nm spectral resolution is achieved over a bandwidth of 400 nm centered at 800 nm, in the designed spectrometer comprised of a plane grating and one freeform mirror. The construction method is advantageous to integrated optic design, and the resulting freeform mirror spectrometer is compact, and simplifies manufacture and alignment.

3.
J Opt Soc Am A Opt Image Sci Vis ; 36(6): 1072-1078, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31158139

RESUMO

The aberrated wavefront propagates along its normal. Both the magnitude and boundary change after the propagation. Wavefronts characterized by Zernike coefficients and a normalized pupil radius can also be represented by a bundle of feature rays normal to the local surface. A ray transfer matrix parameterized by the pupil radius and propagation distance is proposed to transfer these feature rays to obtain the slope and position data of the propagated feature rays. Numerical orthogonal Zernike gradient polynomials are derived to reconstruct the wavefront from the discrete data by using a numerical method. Two aberrated wavefronts are performed as examples to validate the accuracy and flexibility of the proposed numerical method.

4.
J Cell Biochem ; 120(10): 17616-17624, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31119790

RESUMO

AIM: The aim of this study was to explore the role and molecular basis of the long noncoding RNA (lncRNA) TRHDE-AS1 in lung cancer. METHODS: We used real-time polymerase chain reaction to analyze the messenger RNA expression levels of TRHDE-AS1, miR-103, and KLF4. The cell viability, proliferation, and invasion rates were assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Cell Counting Kit-8, and Transwell assays to elucidate the role of TRHDE-AS1. RESULTS: Our results demonstrated that the lncRNA TRHDE-AS1 is mainly located in the cytoplasm and that the cell proliferation and invasion were suppressed in the group of overexpressed TRHDE-AS1. We also showed that miR-103 could directly bind to TRHDE-AS1 and provided evidence of the oncogenic function of miR-103. Besides, we proved that miR-103 exerted its function by adjusting the expression level of the tumor-suppressor gene KLF4, and the expression level was negatively associated with miR-103. CONCLUSION: In summary, we determined that the effects of TRHDE-AS1 on proliferation, invasion, and cell death could be rescued by the overexpression of miR-103. Our experiments demonstrate that the TRHDE-AS1/miR-103/KLF4 axis may provide new evidence for understanding the molecular basis of lung cancer.

5.
Appl Opt ; 58(9): 2269-2276, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31044929

RESUMO

The precision single point diamond turning technique has been a promising technology for generating small and medium-sized freeform optical elements with high surface quality. In this paper, we present an extremely off-axis freeform optical system with a large 10.0 mm pupil diameter and a low 3.0 F-number over a wide 28° field of view. It is composed of two freeform aluminum mirrors, which are fabricated efficiently by the single point diamond turning machine. The manufacturing strategy and parameters are estimated rationally and comprehensively, based on the freeform surface characters. The freeform aluminum mirror surface can reach submicron surface accuracy and achieve nanometer surface roughness. The final assembled prototype of the off-axis two-mirror freeform display optical system has the advantages of compactness, a broad spectrum, and good display imaging performance.

6.
Psychiatry Res ; 274: 66-74, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30780064

RESUMO

Sleep quality has been suggested to play an important role in development of metabolic syndrome (MetS). However, the results have been inconsistent. A systematic review of observational studies aimed to evaluate the association between sleep quality and MetS. A comprehensive search was conducted in PubMed and EMBASE. The pooled odd ratios (ORs) were calculated using random effects models. The construction of funnel plot was used to explore publication bias, with further application of Egger's test. 22 studies were included. The pooled finding showed that overall sleep quality had a significant positive association with MetS (OR 1.37, 95% CI 1.15-1.64), with substantial heterogeneity (I2 = 62.4%, P < 0.1). Similarly, the complaints of sleep, including difficulty in falling sleep (OR 1.18, 95% CI 1.05-1.33), difficulty in maintaining sleep (OR 1.15, 95% CI 1.02-1.30) and sleep inefficiency (OR 1.40, 95% CI 1.04-1.89) were proved to be associated with the risk of MetS. Our results indicated the overall sleep quality as well as sleep complaints have significant positive associations with MetS. Further studies based on a longitudinal design using validated tools that measure both objective and subjective components of sleep quality are needed to explore the causal relationship between sleep quality and MetS.


Assuntos
Síndrome Metabólica/epidemiologia , Transtornos do Sono-Vigília/epidemiologia , Comorbidade , Humanos
7.
Medicine (Baltimore) ; 97(45): e13142, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30407341

RESUMO

Foodborne disease is a major public health concern in both developed and developing countries. China has established a nationwide Foodborne Disease Outbreak Surveillance System (FDOSS) for collection and periodic reporting of data on the occurrence and causes of foodborne disease outbreaks in China. Each provincial Centers for Disease Control and Prevention (CDC) conducts the system working.We reviewed foodborne disease outbreaks that occurred during 2011 to 2016 in Shandong Province from the FDOSS. The Wilcoxon test was used to compare the median number of ill persons in outbreaks. All data analysis was performed using Epi Info 7.During 2011 to 2016, Shandong CDC received reports of 1043 foodborne disease outbreaks, resulting in 8078 illnesses, 2442 hospitalizations, and 17 deaths. There were a median of 69 outbreaks annually [interquartile range (IQR) 10-342], resulting in 335 to 3824 illnesses each year. The median outbreak size was 3 persons (IQR 2-7). Hotels (including cruise ships, hotpot restaurants, barbecue shops) were the most common setting. Among the 744 (71.3%) outbreaks with an implicated food or contaminated ingredient reported, 704 (94.6%) could be assigned to one of 17 predefined commodity categories. Of the 280 outbreaks with a known etiology, 117 (41.8%) were caused by poisonous plants and animals and their toxins, 39 (13.9) were caused by nitrite, and 27 (9.6%) were caused by vibrio parahaemolyticus. Of the 491 (47.1%) outbreaks with at least a contributing factor to cause outbreak, 168 (34.2%) were caused by improper processing, and 100 (20.4) were caused by inedible and misuse.Timely investigation, disposal and reporting of foodborne disease outbreaks provides information that might help FDOSS to make full use of efficiency and FDOSS should be continued and strengthened even more in Shandong Province, such as an increase in diagnostic laboratory capacities.


Assuntos
Surtos de Doenças/estatística & dados numéricos , Doenças Transmitidas por Alimentos/epidemiologia , China/epidemiologia , Doenças Transmitidas por Alimentos/mortalidade , Hospitalização/estatística & dados numéricos , Humanos , Fatores de Risco
8.
Analyst ; 143(16): 3821-3828, 2018 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-30010688

RESUMO

Alkaline phosphatase (ALP) is a significant biomarker for diagnostics. Simple, selective and sensitive detection of ALP activity is thus of critical importance. In this study, an artful fluorescence assay for ALP is proposed based on adenosine triphosphate (ATP) hydrolysis-triggered disassociation and fluorescence quenching of cerium coordination polymer nanoparticles (CPNs). ATP, a recognized natural substrate of phosphatase, can serve as a superb "antenna" to sensitize the luminescence of Ce3+ with the aid of tris(hydroxymethyl) aminomethane (Tris), forming Ce3+-ATP-Tris CPNs. In the presence of ALP, ATP will be catalytically converted into adenosine and inorganic orthophosphate, however neither of them can sensitize Ce3+ in alkaline media. As a result, the obtained CPNs are disassociated, inducing the quenching of the fluorescence. On this basis, a straightforward fluorescence assay for ALP activity is rationally developed. The fluorescence quenching efficiency shows a linear relationship for ALP within the activity range from 0.1 to 10 mU mL-1 with a detection limit of 0.09 mU mL-1 under the optimal experimental conditions. Moreover, this facile yet effective fluorescence method featured simplicity, cost-effectiveness, high sensitivity and high selectivity and can be successfully utilized for the quantitative detection of ALP in human serum samples.


Assuntos
Trifosfato de Adenosina/química , Fosfatase Alcalina/análise , Cério/química , Humanos , Hidrólise , Nanopartículas , Polímeros
9.
Patient Prefer Adherence ; 12: 561-565, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29713146

RESUMO

Objective: To evaluate the prognostic value of the Prehospital Index (PHI) for hospitalized patients with acute trauma. Materials and methods: PHI score and the Injury Severity Score (ISS) were determined in 1,802 hospitalized patients with acute trauma. Receiver-operator characteristic (ROC) curves were used to compare the PHI and ISS in subgroups, and corresponding prediction indicators were calculated. Results: There were significant differences in PHI score and ISS between the survival group and the death group (Z=2.674, P=0.007). The area under the ROC curve was 0.871 (95% CI 0.855-0.886) for PHI score and 0.792 (95% CI 0.773-0.811) for ISS. Optimal cutoff points to determine the risk of critical illness were PHI ≥4 and ISS ≥22. The sensitivity of the PHI was superior to the ISS (χ2=6.975, P=0.008), but the specificity and the accuracy of the PHI and ISS showed no significant difference (P>0.05). Conclusion: The PHI is valuable in prognostic prediction of hospitalized patients with acute trauma, and it is superior to the ISS. The PHI has such advantages as being simple in operation, easy to learn, capable of reflecting conditions timely and reliably, and suitable for dynamic evaluation and screening for critical patients with trauma.

10.
Appl Opt ; 57(2): 263-267, 2018 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-29328174

RESUMO

The ideal mapping geometry in a Fizeau interferometer is to map equal height increments on a flat object and equal angle increments on a spherical surface to equal heights on the detector. So the initial intent of the optical design of Fizeau transmission spheres (TSs) is to provide R-θ mapping geometry for equal angle increments. The corresponding unequal heights mapping will introduce retrace error as coma when linear carrier fringes exist. On the contrary, equal heights mapping with R-sin θ mapping geometry will avoid linear carrier fringes induced coma error. These two different mapping geometries conflict especially for the TS with a small f-number. In this paper, we will first explore the design and the performance of the f/0.75 TS according to the two different mapping geometries, and then evaluate the mapping geometry for the commercial ZYGO f/0.75 TS, and give some engineering notes for the designers, the metrologists, and the fabricators in the optical laboratory.

11.
Appl Opt ; 56(33): 9341-9350, 2017 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-29216108

RESUMO

Due to its advantages of nonintrusiveness, wide measurement range, and insensitivity to variation, Moiré deflectometry is a powerful tool for quantitative measurement of a flow field's physical parameters such as density and temperature. Moiré volume computed tomography (MVCT), combining the moiré deflectometry and volume optical computed tomography (VOCT), can realize real three-dimensional parameters reconstruction, in which the radial derivatives extraction of the projected phase is of great importance. In this paper, a spatial phase-shifting-interferometry-based MVCT system was proposed to extract the radial shearing phase distribution. The system is simple and compact, and consists of three crossed gratings and lenses, with no wave plates or polarizers introduced. Via using a 4-f system, the optical path was shortened, and four spatial phase-shifting grid moiré projections can be obtained simultaneously. Each grid interferogram was filtered and separated into two linear interferograms in two orthogonal directions. Moreover, a two-step spatial phase-shifting algorithm was applied to obtain the first-order derivative phase in two mutually perpendicular directions, respectively. Simulations were implemented to verify the feasibility and accuracy of the proposed phase retrieval method. The measured results for the radial first-order partial derivative of the phase projection of a propane flame in the experimental VOCT system are presented.

12.
Iran J Public Health ; 46(9): 1223-1230, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29026788

RESUMO

BACKGROUND: China had implemented policies to limit antimicrobials prescription since 2004; we conducted this study to reflect the effect of these national policies by analyzing antimicrobial prescription trends of medical insurance in patients from 2003 to 2014 in Changsha city, China. METHODS: The participants were inpatients of the medical insurance of urban workers (UEBMI). Data were extracted from medical insurance information system of Changsha Medical Insurance Institution, which directly connects with hospitals information systems. RESULTS: Trend analysis showed great changes in antimicrobial prescription and inpatients' cost on antimicrobials over the study period. Antimicrobial prescription rates gradually declined over the study period from 79.0% in 2003 to 43.5% in 2014 (adjusted OR0.205; 95%CI 0.198 to 0.213). There was a quicker decline from 2011 to 2014 (with implementing national antimicrobial stewardship action plan) than the period from 2003 to 2010 (with implementing antimicrobials use education and self-management strategies). The proportion of inpatients used one antimicrobial increased significantly from 25.6% in 2003 to 46.7% in 2014, while the proportion of inpatients used three or more antimicrobials gradually decreased. Bacterial culture rate increased from 20.4% in 2003 to 36.6% in 2014 (adjusted OR 2.248; 95% CI 2.149 to 2.352). The average costs on antimicrobials decreased significantly, from 277.43 US Dollar in 2003 to 91.05 US Dollar in 2014. CONCLUSION: National efforts to promote rational use of antimicrobials in clinical practice have had a positive effect over the past decade in China.

13.
Opt Express ; 25(21): 26133-26147, 2017 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-29041274

RESUMO

In this paper, a generalized shift-rotation absolute measurement method is proposed to measure the absolute surface shape of high-numerical-aperture spherical surfaces. Based on the wavefront difference method, the high order misalignment aberrations can be removed from the measurements. Our generalized shift-rotation absolute measurement process only needs one rotational measurement position and one translational measurement position. A wavefront reconstruction method based on the self-adaptive differential evolution algorithm is proposed to calculate the Zernike polynomials coefficient ai of the absolute surface shape Wtest(x,y), the rotation angle Δθ, the translation δx along the x axis, and the translation δy along the y axis. The translation error and rotation error in other absolute measurement methods are avoided using our generalized shift-rotation absolute measurement method. Experimental absolute results of the test surface and reference surface are given and the difference of reference surface shapes between two testings in experiments is 0.12 nm root mean square.

14.
Appl Opt ; 56(10): 2574-2579, 2017 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-28375215

RESUMO

We utilize the spectral broadening of Yb-doped fiber lasers in the spectral beam combining scheme to develop an analytical model of the coupling efficiency, which forms a critical factor in evaluating the practicality of the beam combination system. The simulation results predict a trend similar to the measured ones. Via increasing the number of simulating lasers, the model can be extended to calculate the combining efficiency of the resulting multiple-beam-combination system and estimate the optimal output power and combining efficiency. Moreover, the analytical model is suitable to investigate key parameters of Yb-doped lasers and filters, which is beneficial in enhancing the combining efficiency.

15.
J Diabetes Res ; 2017: 2740372, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28271075

RESUMO

Objectives. We performed continuous glucose monitoring (CGM) to define the features of patients with newly diagnosed type 2 diabetes (T2D) before and after Continuous Subcutaneous Insulin Infusion (CSII) therapy. Methods. This was a retrospective analysis. Newly diagnosed T2D patients (106) were admitted from eight centers in China. They were divided into a younger patient group (<60 years) and an older patient group (≥60 years). Each group was further divided into male and female patients. CSII therapy was maintained for 3 weeks after the glycemic target was reached. CGM was performed 2 times before and after completion of insulin treatment. Results. CGM data showed the expected significant improvement of mean amplitude glycemic excursion (MAGE) with CSII therapy. The older patients had lower hourly glucose concentrations from 0200 to 0700 o'clock compared to the younger patients at baseline. Surprisingly, in the older patient group, the male patients had a potential risk of hypoglycemia after CSII therapy, especially during periods from 2300 to 2400 and 0400 to 0600. Conclusions. Our data suggested that older male patients with newly diagnosed T2D may have lower nocturnal glucose concentrations. This may potentially increase the risk of nocturnal hypoglycemia during CSII therapy. This study was registered with Chinese Clinical Trial Registry, number CliCTR-TRC-11001218.


Assuntos
Glicemia/análise , Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemia/induzido quimicamente , Hipoglicemiantes/efeitos adversos , Insulina/efeitos adversos , Adulto , Idoso , Diabetes Mellitus Tipo 2/sangue , Esquema de Medicação , Feminino , Humanos , Hipoglicemia/sangue , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/uso terapêutico , Infusões Subcutâneas , Insulina/administração & dosagem , Insulina/uso terapêutico , Sistemas de Infusão de Insulina , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco
16.
Appl Opt ; 55(17): 4769-75, 2016 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-27409038

RESUMO

We have proposed a virtual quadratic Newton rings phase-shifting moiré-fringes measurement method in a nonnull interferometer to measure the large radius of curvature for a spherical surface. In a quadratic polar coordinate system, linear carrier testing Newton rings interferogram and virtual Newton rings interferogram form the moiré fringes. It is possible to retrieve the wavefront difference data between the testing and standard spherical surface from the moiré fringes after low-pass filtering. Based on the wavefront difference data, we deduced a precise formula to calculate the radius of curvature in the quadratic polar coordinate system. We calculated the retrace error in the nonnull interferometer using the multi-configuration model of the nonnull interferometric system in ZEMAX. Our experimental results indicate that the measurement accuracy is better than 0.18% for a spherical mirror with a radius of curvature of 41,400 mm.

17.
Appl Opt ; 55(8): 1920-8, 2016 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-26974783

RESUMO

Multi-beam interference exists in testing high-reflectivity surfaces with a Fizeau interferometer. In this paper, the multi-beam interference intensity was estimated as the sum of the first six order harmonics using the Fourier series expansion. Then, by adopting carrier squeezing interferometry with a π/4 phase shift, an algorithm was proposed to extract the phase from multi-beam interferograms. To ensure the separation of the lobes of phase-shift errors and the phase in the frequency domain, conditions of the necessary linear carrier in the proposed algorithm were derived. Simulation results indicated that the phase retrieving precision is better than PV 0.008λ and RMS 0.001λ, even when the reflection coefficient of the test surface is as high as 0.9 and the phase shift varies within π/4±π/20. Compared with the other algorithms, the proposed algorithm for multi-beam interference was validated by its good performance in the experiments, especially when the phase-shift error exists.

18.
Appl Opt ; 54(32): 9554-62, 2015 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-26560786

RESUMO

Free-space Fourier domain optical coherence tomography is adopted for biomedical imaging with ultrahigh resolution, in which the setup consists of an interferometer and a spectrometer. Two-dimensional lateral sampling in the sample arm of the interferometer is achieved by using a galvanometer-based scanner. Optical path difference (OPD) drift in the full scan field of view is observed in the assembly process of the scanner. A galvo mirror mount offset with respect to the rotation axis is demonstrated as the derivation of this OPD drift by both geometric analyses and model building. Then, an iterative assembly process of the scanner is proposed with the OPD drift taken as the alignment criteria.


Assuntos
Análise de Fourier , Aumento da Imagem/instrumentação , Lentes , Radiometria/instrumentação , Tomografia de Coerência Óptica/instrumentação , Calibragem , Desenho de Equipamento , Análise de Falha de Equipamento , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
19.
Opt Express ; 23(20): 26208-20, 2015 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-26480134

RESUMO

Wavefront estimation from the slope-based sensing metrologies zis important in modern optical testing. A numerical orthogonal transformation method is proposed for deriving the numerical orthogonal gradient polynomials as numerical orthogonal basis functions for directly fitting the measured slope data and then converting to the wavefront in a straightforward way in the modal approach. The presented method can be employed in the wavefront estimation from its slopes over the general shaped aperture. Moreover, the numerical orthogonal transformation method could be applied to the wavefront estimation from its slope measurements over the dynamic varying aperture. The performance of the numerical orthogonal transformation method is discussed, demonstrated and verified by the examples. They indicate that the presented method is valid, accurate and easily implemented for wavefront estimation from its slopes.

20.
Appl Opt ; 54(22): 6857-64, 2015 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-26368102

RESUMO

An achromatic transmission sphere (TS) must be provided as an accessory for an infrared interferometer with a broadband wavelength channel. When designing an F/0.75 infrared TS using the one-asphere model of the two-lens-only package, the first surface was optimized as a strong asphere beyond our current testing ability, using either a slope-unconstrained Qbfs asphere equal to a conventional even asphere, or using a slope-constrained Qbfs asphere. We applied the asphere-splitting theory to redistribute the asphericity. Adopting only the slope-constrained Qbfs asphere, the strong asphere was successfully split into two weaker aspheres within our testing ability, getting a more manufacturable two-asphere TS solution. Besides, the TS solution with two weaker aspheres presented the same sensitivity to assembly errors compared to the solution with one strong asphere, as demonstrated by the analysis of the wavefront aberrations of the TS solutions. This phenomenon coincides well with the asphere-splitting theory, given that the total asphericity remains almost equivalent between the two solutions.

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