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1.
J Med Internet Res ; 22(1): e13201, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32012054

RESUMO

BACKGROUND: Affective states, including sentiment and emotion, are critical determinants of health. However, few studies among men who have sex with men (MSM) have examined sentiment and emotion specifically using real-time social media technologies. Moreover, the explorations on their associations with sexual and health status among MSM are limited. OBJECTIVE: This study aimed to understand and examine the associations of affective states with sexual behaviors and health status among MSM using public data from the Blued (Blued International Inc) app. METHODS: A total of 843,745 public postings of 377,610 MSM users located in Guangdong were saved from the Blued app by automatic screen capture. Positive affect, negative affect, sexual behaviors, and health status were measured using the Simplified Chinese Linguistic Inquiry and Word Count. Emotions, including joy, sadness, anger, fear, and disgust, were measured using the Weibo Basic Mood Lexicon. A positive sentiment score and a positive emotion score were also calculated. Univariate and multivariate linear regression models on the basis of a permutation test were used to assess the associations of affective states with sexual behaviors and health status. RESULTS: A total of 5871 active MSM users and their 477,374 postings were finally selected. Both positive affect and positive emotions (eg, joy) peaked between 7 AM and 9 AM. Negative affect and negative emotions (eg, sadness and disgust) peaked between 2 AM and 4 AM. During that time, 25.1% (97/387) of negative postings were related to health and 13.4% (52/387) of negative postings were related to seeking social support. A multivariate analysis showed that the MSM who were more likely to post sexual behaviors were more likely to express positive affect (beta=0.3107; P<.001) and positive emotions (joy: beta=0.027; P<.001), as well as negative emotions (sadness: beta=0.0443; P<.001 and disgust: beta=0.0256; P<.001). They also had a higher positive sentiment score (beta=0.2947; P<.001) and a higher positive emotion score (beta=0.1612; P<.001). The MSM who were more likely to post their health status were more likely to express negative affect (beta=0.8088; P<.001) and negative emotions, including sadness (beta=0.0705; P<.001), anger (beta=0.0058; P<.001), fear (beta=0.0052; P<.001), and disgust (beta=0.3065; P<.001), and less likely to express positive affect (beta=-0.0224; P=.02). In addition, they had a lower positive sentiment score (beta=-0.8306; P<.001) and a lower positive emotion score (beta=-0.3743; P<.001). CONCLUSIONS: The MSM social media community mainly expressed their positive affect in the early morning and negative affect after midnight. Positive affective states were associated with being sexually active, whereas negative affective states were associated with health problems, mostly about mental health. Our finding suggests the potential to deliver different health-related intervention strategies (eg, psychological counseling and safe sex promotion) on a social media app according to the affective states of MSM in real time.

2.
Tissue Eng Part A ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031056

RESUMO

The recent advent of 3D bioprinting of biopolymers provides a novel method for fabrication of tissue-engineered scaffolds, and also offers a potentially promising avenue in cartilage regeneration. Silk fibroin (SF) is one of the most popular biopolymers used for 3D bioprinting, but further application of SF is hindered by its limited biological activities. The incorporation of growth factors has been identified as a solution to improve the biological function. Platelet-rich plasma (PRP) is an autologous resource of growth factors, which has been widely used in clinic. In this study, we have developed SF-based bioinks incorporated with different concentrations of PRP (12.5%, 25%, and 50%; vol/vol). Release kinetic studies show that SF-PRP bioinks could achieve controlled release of growth factors. Subsequently, SF-PRP bioinks were successfully fabricated into scaffolds by bioprinting. Our results revealed that SF-PRP scaffolds possessed proper internal pore structure, good biomechanical properties, and a suitable degradation rate for cartilage regeneration. Live/dead staining showed that 3D-printed SF-PRP scaffolds were biocompatible. Moreover, in vitro studies revealed that tissue-engineered cartilage from the SF-PRP group exhibited improved qualities as compared with the pure SF controls, according to the histological and the immunohistochemical findings. Biochemical evaluations confirmed that SF-PRP (50% PRP, v/v) scaffolds allowed the largest increases in collagen and glycosaminoglycan concentrations, when compared with the pure SF group. These findings suggest that 3D-printed SF-PRP scaffolds could be potential candidates for cartilage tissue engineering.

3.
J Colloid Interface Sci ; 565: 465-473, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31982713

RESUMO

Metal-organic frameworks (MOFs) based membranes with superior mechanical properties are of particular interest in purification, separation, and catalysis. Nevertheless, their fabrication still remains a grand challenge. Here, fungus hyphae (Mucor) were used as a robust scaffold to load the MOFs and induced the formation of porous and flexible membranes. ZIF-8 was used as a representative of MOFs. The ZIF-8@Mucor membrane was formed by the in-situ growth of ZIF-8 on hyphae and then a vacuum filtration of the ZIF-8/hyphae composite. ZIF-8 was effectively dispersed on the ZIF-8@Mucor membrane, and the shear modulus of ZIF-8@Mucor-3 was 864 MPa by calculation. The ZIF-8@Mucor membrane exhibited promising properties for adsorption application to remove the highly toxic Pb2+. The adsorption capacity of this membrane was as high as 1443.29 mg/g. Results from dynamic adsorption indicated that the penetration concentration of Pb2+ ions was less than 5% of the original level before 80 min whereas after 160 min, penetration concentration of Pb2+ ions was more than 90%. This study would open a new way of how to synthesize composite MOFs/bacterial membranes for energy and environment purposes.

4.
World Neurosurg ; 136: 66-69, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31931251

RESUMO

BACKGROUND: Giant cell reparative granuloma (GCRG) is a rare benign tumor. The jawbone is the most common site of occurrence, followed by sphenoid bone, craniofacial bone, hand and foot bones. The etiology of GCRG is unknown but may be related to an intraosseous hemorrhage following trauma. Despite its benign nature, it could be locally aggressive. To our knowledge, no spinal epidural GCRG case has been reported. CASE DESCRIPTION: A case of man aged 32 years who presented with upper right limb numbness and weakness. Computed tomography showed a round soft tissue mass in the spinal canal at the C7-T1 level. The mass showed isointensity on T1-weighted images, hypointensity on T2-weighted images, and significant enhancement on postcontrast T1-weighted images. The mass localized in the epidural space and was surgically resected. The histologic diagnosis was consistent with GCRG. CONCLUSIONS: Spinal epidural GCRG is rare and is hardly considered in the differential diagnosis. Preoperative diagnosis of GCRG is challenging, and the definitive diagnosis could only be made by pathological examination. Surgical resection is probably an effective therapy for relief of symptoms.

5.
J Orthop Surg (Hong Kong) ; 28(1): 2309499019896026, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31965906

RESUMO

PURPOSE: Pneumatic tourniquets are used in total knee arthroplasty (TKA) for surgical field visualization and improved cementation; however, their use is controversial. This study aimed to assess the effects of tourniquet application on enhanced recovery post-TKA. METHODS: A prospective randomized single-blinded trial assessed tourniquet's effects on postoperative pain, swelling, and early outcome in TKA. One-hundred and two patients with knee osteoarthritis were randomized to full-course (FC) and second half-course (SHC) application (n = 51/group). Tumor necrosis factor-alpha (TNF-α), C-C motif chemokine ligand 2(CCL-2), pentraxin-3 (PTX-3), prostaglandin E-2 (PGE-2), superoxide dismutase-1 (SOD-1), and myoglobin (Mb) were assessed by enzyme-linked immunosorbent assay, while the visual analog scale (VAS), range of motion (ROM), and thigh circumference growth rate were recorded. RESULTS: Average tourniquet duration significantly differed between the SHC (37.5 ± 5.1 min) and FC (66.4 ± 7.2 min) groups (p < 0.01); VAS and thigh circumference growth rate in the SHC group were much lower compared with the FC group, while ROM was higher within 48 h of tourniquet removal (p < 0.01). Blood TNF-α, PTX3, CCL2, PGE2, SOD-1, and Mb were lower in the SHC group than the FC group (p < 0.01). Additionally, intraoperative blood loss was significantly elevated in the SHC group than the FC group (p < 0.01), with lower postoperative blood loss in the drain (p = 0.001). Postoperative drainage volume was reduced in the SHC group compared with the FC group (p < 0.01); five and two patients in the FC and SHC groups required blood transfusion, respectively (p = 0.025). Hospital stay tended to be shorter in the SHC group (p = 0.023), and no tourniquet-related complications were recorded. CONCLUSION: Improved therapeutic outcome was observed in the SHC group, indicating patients should routinely undergo TKA with SHC tourniquet application.

6.
Molecules ; 25(3)2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31979069

RESUMO

A new triterpenoid saponin, named terpengustifol A (1), and two new lignan glucosides, phengustifols A and B (2 and 3), were isolated from the flowers of Elaeagnus angustifolia. Their structures were determined by the extensive analysis of the spectroscopic data (including NMR and HRMS) and ECD calculations. Compound 1 possesses an unusual monoterpene (Z)-6-hydroxy-2,6-dimethylocta-2,7-dienoyl unit at C-21. Compounds 2 and 3 are a pair of diastereoisomers, while their aglycones are a pair of enantiomers. Compounds 1 and 2 exhibited moderate cytotoxic activities against A375 cell lines with IC50 values at 12.1 and 15.6 µM, respectively. This is firstly reported the triterpenoid saponin and lignans isolated from the Elaeagnus angustifolia flowers.

7.
World J Pediatr ; 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31912317

RESUMO

BACKGROUND: Hearing impairment is one of the most common birth defects in children. Universal newborn hearing screenings have been performed for 19 years in Guangdong province, China. A screening/diagnosis/intervention system has gradually been put in place. Over the past 10 years, a relatively complete data management system had been established. In the present study, an etiological analysis of newborn cases that failed the initial and follow-up screenings was performed. METHODS: The nature and degree of hearing impairment in newborns were confirmed by a set of procedures performed at the time of initial hearing screening, rescreening and final hearing diagnosis. Then, multiple examinations were performed to explore the associated etiology. RESULTS: Over a period of 10 years, 720 children were diagnosed with newborn hearing loss. Among these children, 445 (61.81%) children had a clearly identified cause, which included genetic factor(s) (30.56%), secretory otitis media (13.30%), maternal rubella virus infection during pregnancy (5.83%), inner ear malformations (4.86%), maternal human cytomegalovirus infection during pregnancy (2.92%), malformation of the middle ear ossicular chain (2.50%) and auditory neuropathy (1.81%). In addition, 275 cases of sensorineural hearing loss of unknown etiology accounted for 38.19% of the children surveyed. CONCLUSIONS: Long-term follow-up is needed to detect delayed hearing impairment and auditory development in children. The need for long-term follow-up should be taken into account when designing an intervention strategy. Furthermore, the use of the deafness gene chip should further elucidate the etiology of neonatal hearing impairment.

8.
Int J Hyg Environ Health ; 224: 113442, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31978737

RESUMO

BACKGROUND: Shared bicycles offer the potential to be sources for interindividual bacterial community transfer. However, the overall ecology of the bacterial community persisting on the shared bicycles is still unknown. METHODS: We used 16S rRNA sequencing techniques in combination with multiple bioinformatic analyses to profile bacterial communities on shared bicycle handles and saddles in selected five locations in a metropolitan area (Chengdu, China). Samples of air around shared bicycles were collected as a control. RESULTS: We demonstrate that the species richness and diversity of samples from shared bicycles are significantly higher than those of surrounding air samples. Taxonomical composition indicates that community structure of shared bicycle handles and saddles are also different. Additionally, high occurrence of pathogenic or potentially pathogenic genera is found on the surfaces of shared bicycles, including Sphingomonas, Acinetobacter, Staphylococcus, and Cutibacterium. Moreover, PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) further verifies that bacteria involved in signaling pathways related to human diseases are at significantly higher levels in shared bicycle samples, while bacteria associated with environmental information processing pathways are at significantly higher levels in the air samples. CONCLUSION: Our results reveal the profiles of bacterial communities on shared bicycles, and suggest that potentially pathogenic bacteria may impair human health through shared bicycles, especially in susceptible individuals. Notably, this investigation will prompt the public to pay more attention to the management of bicycle sharing programs worldwide, and it also provides research basis for the government to formulate corresponding public health policies.

9.
Mol Oncol ; 14(1): 197-210, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31721429

RESUMO

Hepatocellular carcinoma (HCC) has emerged as one of the most prevalent life-threatening cancers, and the high mortality rate is largely due to the metastasis. The sustained activation of Smad4 and transforming growth factor-ß (TGF-ß) is closely associated with advanced HCC metastasis. However, the regulatory mechanism underlying the aberrant activation of Smad4 and TGF-ß pathway remains elusive. In this study, using a functional screen of USPs siRNA library, we identified ubiquitin-specific proteases USP10 as a deubiquitinating enzyme (DUB) that sustains the protein level of Smad4 and activates TGF-ß signaling. Further analysis showed that USP10 directly interacts with Smad4 and stabilizes it through the cleavage of its proteolytic ubiquitination, thus promoting HCC metastasis. The suppression of USP10 by either shRNAs or catalytic inhibitor Spautin-1 significantly inhibited the migration of HCC cells, whereas the reconstitution of Smad4 was able to efficiently rescue this defect. Overall, our study not only uncovers the regulatory effect of USP10 on the protein abundance of Smad4, but also indicates that USP10 could be regarded as a potential intervention target for the metastatic HCC in Smad4-positive patients.

10.
Antonie Van Leeuwenhoek ; 113(1): 147-154, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31542849

RESUMO

A Gram-stain-negative, rod-shaped and aerobic bacterium, designated CL12T, was isolated from roots of Glycine max (Linn. Merr.) collected from an experimental field in the campus of South China Agricultural University, PR China (22°58'46″S, 110°51'10″E). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CL12T belongs to the genus Rhizobium, closely related to Rhizobium wuzhouense W44T (99.3%), followed by Rhizobium rosettiformans W3T (98.0%) and Rhizobium ipomoeae Shin9-1T (97.9%). The results of analysis of sequences of four housekeeping genes (recA, atpD, rpoB and glnA) also revealed strain CL12T to be closely related to R. wuzhouense W44T with the similarities 91.0%, 95.0%, 94.2% and 90.5%, respectively. The major fatty acid of strain CL12T was Summed Feature 8 (C18:1ω7c and/or C18:1ω6c). Strain CL12T had not the nodulation genes (nodC and nodA) and nitrogenase reductase gene (nifH), and could not cause formation of nodule on soybean. The draft genome size of strain CL12T was 4.84 Mbp with a genomic DNA G + C content of 61.1 mol%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) of strain CL12T and R. wuzhouense W44T were 27.4% and 84.7%, respectively. Based on genomic, phenotypic and phylogenetic analysis, strain CL12T is suggested to represent a new species of the genus Rhizobium, for which the name Rhizobium glycinendophyticum sp. nov. is proposed. The type strain is CL12T (=GDMCC 1.1597T = KACC 21281T).

11.
Cytometry A ; 97(1): 24-30, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31313517

RESUMO

We develop a single-mode fiber-based cytometer for the obtaining of two-dimensional (2D) light scattering patterns from static single cells. Anisotropy of the 2D light scattering patterns of single cells from ovarian cancer and normal cell lines is investigated by histograms of oriented gradients (HOG) method. By analyzing the HOG descriptors with support vector machine, an accuracy rate of 92.84% is achieved for the automatic classification of these two kinds of label-free cells. The 2D light scattering anisotropy cytometry combined with machine learning may provide a label-free, automatic method for screening of ovarian cancer cells, and other types of cells. © 2019 International Society for Advancement of Cytometry.

12.
Eur J Gastroenterol Hepatol ; 32(1): 120-126, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31688313

RESUMO

OBJECTIVES: In longitudinal studies, serum biomarkers are often measured longitudinally which is valuable to predict the risk of disease progression. Previous risk prediction models for liver cirrhosis restrict data to baseline or baseline and a single follow-up time point, which failed to incorporate the time-dependent marker information. The aim of this study is to develop risk model in patients with chronic hepatitis B for dynamic prediction of cirrhosis by incorporating longitudinal clinical data. METHODS: Data from the hospital-based retrospective cohort at the Third Affiliated Hospital of Sun Yat-sen University, from 2004 to 2016, were analyzed. Using the multilevel logistic regression model, the time-dependent marker information and individual characteristics were taken as input, and the risk of at different time as the output. RESULTS: At the end of follow-up, 8.8% of patients progressed to cirrhosis, the average estimate values of hepatitis B virus DNA and alanine aminotransferase demonstrated a downward trend, the aspartate aminotransferase/alanine aminotransferase ratio showed a flat trend overall. The important predictors were as follows: age, oral antiviral treatment, hepatitis B virus DNA. This risk prediction model had an area under the receiver operator characteristic curve of 0.835 (95% confidence interval: 0.772-0.899) and 0.809 (95% confidence interval: 0.708-0.910) in the derivation and validation sets, respectively. CONCLUSION: Longitudinal prediction model can be used for dynamic prediction of disease progression and identify changing high-risk patients.

13.
Diabetol Metab Syndr ; 11: 96, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31788033

RESUMO

Background: To investigate effects of metformin on the regulation of proteins of white adipose tissue (WAT) and brown adipose tissue (BAT) in obesity and explore the underlying mechanisms on energy metabolism. Methods: C57BL/6J mice were fed with normal diet (ND, n = 6) or high-fat diet (HFD, n = 12) for 22 weeks. HFD-induced obese mice were treated with metformin (MET, n = 6). After treatment for 8 weeks, oral glucose tolerance test (OGTT) and hyperinsulinemic-euglycemic clamp were performed to evaluate the improvement of glucose tolerance and insulin sensitivity. Protein expressions of WAT and BAT in mice among ND, HFD, and MET group were identified and quantified with isobaric tag for relative and absolute quantification (iTRAQ) coupled with 2D LC-MS/MS. The results were analyzed by MASCOT, Scaffold and IPA. Results: The glucose infusion rate in MET group was increased significantly compared with HFD group. We identified 4388 and 3486 proteins in WAT and BAT, respectively. As compared MET to HFD, differential expressed proteins in WAT and BAT were mainly assigned to the pathways of EIF2 signaling and mitochondrial dysfunction, respectively. In the pathways, CPT1a in WAT, CPT1b and CPT2 in BAT were down-regulated by metformin significantly. Conclusions: Metformin improved the body weight and insulin sensitivity of obese mice. Meanwhile, metformin might ameliorate endoplasmic reticulum stress in WAT, and affect fatty acid metabolism in WAT and BAT. CPT1 might be a potential target of metformin in WAT and BAT.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31828712

RESUMO

The present research developed a direct in situ heterogeneous method to synthesize UiO-66-poly(m-phenylenediamine) core-shell nanostructures by inducing assembly of m-phenylenediamine radical on UiO-66 surfaces. The strong interaction between negative charged UiO-66 and positive radical from the oxidation of monomer is the major driving force. The produced UiO-66-poly(m-phenylenediamine) composites exhibited a distinct core-shell morphology with controllable surface features. The UiO-661-PmPD0.5 showed a uniform PmPD shell with a thickness of 40-60 nm and the nanocomposite exhibited a high specific surface area of 319.77 m2 g-1. Moreover, the Cr(VI) adsorption amount of the polymeric shell in the nanocomposites can reach as high as 745 mg g-1, far beyond the performance of the original PmPD. The adsorption tends to be equilibrium within 300 min. This research opens a hopeful window for facile and large-scale fabrication of core-shell nanostructures with controllable core-shell configuration, exhibiting high prospect in heavy metal removal from water.

15.
Onco Targets Ther ; 12: 10199-10211, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819509

RESUMO

Purpose: This study was conducted to investigate the differentially expressed profiles of long non-coding RNAs (lncRNAs) in HBV-associated HCC (HBV-HCC), which may serve as potential diagnostic biomarkers and therapeutic targets. Methods: To examine the differentially expressed profiles of lncRNAs and mRNAs using microarray analysis, we collected 15 specimens: five HBV-associated HCC tissues, five paired adjacent peritumoral liver tissues (APLT), and five distant peritumoral liver tissues (DPLT). Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to predict the biological roles and potential signaling pathways of these dysregulated mRNAs. In addition, lncRNA-mRNA co-expression network and signal transduction pathway network (Signal-net) were employed to further explore the potential target genes and roles of dysregulated lncRNAs in HBV-HCC pathogenesis. Finally, quantitative real-time PCR (qRT-PCR) was used to confirm the expression of six selected dysregulated lncRNAs. Results: A total number of 719 lncRNAs and 3438 mRNAs were significantly more dysregulated in HBV-HCC tissues than in APLT and DPLT (fold change > 2, P < 0.05, FDR < 0.05). Additionally, 337 GO terms and 53 KEGG pathways were established to be significantly enriched. These dysregulated mRNAs were mainly enriched in metabolism-related biological processes. Additionally, lncRNA-mRNA coexpression network analysis showed that NONHSAT053785 is at the core of the network. Furthermore, the Signal-net analysis showed that CYP3A4 was gene with the highest degree. Finally, the data of five of the six selected differentially expressed lncRNAs were in agreement with the microarray data obtained by qRT-PCR verification. Conclusion: Our study revealed the differentially expressed profiles of lncRNAs and mRNAs for HBV-HCC, and five novel dysregulated lncRNAs were identified in HBV-HCC tissues. The aforementioned dysregulated lncRNAs may represent potential diagnostic biomarkers and therapeutic targets of HBV-HCC, which needs to be validated in future studies.

16.
Oncol Lett ; 18(6): 6459-6468, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31819776

RESUMO

Heterogeneous ribonucleoprotein AB (hnRNP AB) is a member of the heterogeneous nuclear ribonucleoprotein family, which serves important functions in gene expression and signal transduction. However, the expression and clinicopathological significance of hnRNP AB in colorectal cancer (CRC) remain to be elucidated. To investigate the expression and clinical significance of hnRNP AB in CRC, hnRNP AB expression levels were analysed in two independent cohorts of patients with CRC. The results of reverse transcription-quantitative PCR, immunohistochemistry and western blot analysis demonstrated that hnRNP AB was upregulated in CRC tissues compared with the corresponding adjacent normal tissues. Immunohistochemical analyses indicated that a high expression of hnRNP AB was significantly associated with preoperative carcinoembryonic antigen (CEA; P<0.001) and carbohydrate antigen 19-9 (P=0.014) levels, tumour size (P=0.022) and infiltration (P=0.026), lymph node metastasis (P<0.001) and Tumour-Node-Metastasis stage (P<0.001). Univariate and multivariate Cox survival analyses revealed that hnRNP AB expression and preoperative CEA levels were significant independent factors affecting overall survival in patients with CRC (P<0.05). According to the Kaplan-Meier model, patients with CRC with high hnRNP AB expression exhibited significantly poorer prognosis compared with those with low hnRNP AB expression (P<0.001). In conclusion, the results of the present study demonstrated that hnRNP AB expression may serve an important role in the progression of CRC and that hnRNP AB may be considered a predictor of prognosis for patients with CRC.

17.
J Chem Ecol ; 2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31853816

RESUMO

Chemosensory proteins (CSPs) are thought to play roles in the insect olfactory system by binding and carrying hydrophobic odorants across the aqueous sensillar lymph. The band-winged grasshopper, Oedaleus asiaticus Bei-Bienko, is one of the most important grasshopper pests in northern China, but there is little information about its olfactory system. In order to investigate the olfactory functions of CSPs in this pest, three CSP genes (OasiCSP4, OasiCSP11 and OasiCSP12) were expressed in Escherichia coli, and the binding affinities of the three recombinant CSP proteins were measured for 16 volatiles from the host plant (Stipa krylovii), fecal material and body of live adult O. asiaticus using fluorescence competitive binding assays. To further verify their olfactory functions, RNA interference (RNAi) and electrophysiological recording were conducted. The three recombinant proteins displayed different degrees of binding to various volatiles in ligand-binding assays, with OasiCSP12 having higher binding affinities for more volatiles than OasiCSP4 and OasiCSP11. OasiCSP12 exhibited strong binding affinities (Ki < 20 µΜ) for five host plant volatiles and one volatile from the live body of adult O. asiaticus. The transcript levels of the three OasiCSP genes were significantly lower after silencing the individual genes by RNAi, which in turn reduced the EAG responses in adults of both sexes to most tested compounds. Our study indicates that these three OasiCSPs are involved in the detection of volatile semiochemicals, and may play important roles in finding host plants and in aggregation in O. asiaticus.

18.
Materials (Basel) ; 12(23)2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31779198

RESUMO

Ultra-thin plates have great potential for applications in aircraft skin, the packaging industry, and packaging of electronic products. Herein, 1 mm-thick 5A06 Al alloy was welded with friction stir welding. The microstructural evolution of the welds was investigated in detail with optical microscopy, scanning electron microscopy, and electron backscatter diffraction. The results showed that the friction stir welds of 1 mm-thick 5A06 Al alloy were well formed without obvious defect and with a minimum thickness reduction of 0.025 mm. Further, the grain size and the proportion of low-angle grain boundaries decreased with decreasing welding speed, because of the increasing degree of dynamic recrystallization. Among all of the welded joints, the welding speed of 100 mm/min yielded the smallest grain size and the highest proportion of high-angle grain boundaries, and thus the best mechanical properties. Specifically, the tensile strength of the joint was greater than that of the base material, while the elongation reached 80.83% of the base material.

19.
Sci Rep ; 9(1): 18003, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31767952

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

20.
J Agric Food Chem ; 67(50): 13960-13968, 2019 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-31751508

RESUMO

This study aimed to evaluate the antiglycation ability of Elaeagnus angustifolia flower extract and to elucidate the mechanism with its major compound. The results indicated that E. angustifolia flower extract and its major compound tiliroside (24.2 mg/g of extract) exhibited excellent antiglycation ability with inhibition rates of 92.1 and 78.9% at 37.5 µg/mL, which are much higher than that of aminoguanidine (55.3% at 37.5 µg/mL). The stable tiliroside-ovalbumin (OVA) complexes were formed through a spontaneous exothermic progress in an equimolar manner, and hydrophobic interaction, hydrogen bond, and van der Waals forces were the major driving forces. Tiliroside could significantly ameliorate the conformation changes of OVA induced by the glycation reaction, quench its fluorescence by a static mechanism, and change the microenvironment adjacent to tryptophan and tyrosine. Molecular docking revealed that tiliroside inserted into the OVA hydrophobic pocket resulted in the formation of five hydrogen bonds. Orbitrap tandem mass spectrometry showed that tiliroside significantly suppressed the glycation of OVA, and the number of glycation sites was reduced from 9 to 5 after tiliroside was added. The above results indicated that E. angustifolia flowers and tiliroside have a good antiglycation effect and can be used as food additives to suppress the undesired glycation reaction during food processing.


Assuntos
Elaeagnaceae/química , Flavonoides/química , Extratos Vegetais/química , Flores/química , Glicosilação , Simulação de Acoplamento Molecular , Estrutura Molecular
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