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1.
Sci Total Environ ; : 161872, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36716873

RESUMO

This study systematically investigated the occurrence, spatiotemporal distribution, and ecological risk of 106 pesticides in the surface water of the Jiaodong Peninsula in China. The results show that 52 pesticides, including 21 insecticides, 10 fungicides, and 21 herbicides, were detectable in the surface water. The concentrations of target pesticides in water samples ranged from 0.42 (tebuconazole in the wet season) to 645.31 ng/L (thiamethoxam in the normal season). The two most polluting and widespread pesticides were quintozene (maximum concentration of 481.46 ng/L and detection rate of 93.46 %) and atrazine (maximum concentration of 465.73 ng/L and detection rate of 100 %). The total pesticide concentrations in surface water in different seasons revealed the order of dry season > wet season > normal season. Based on aquatic pesticide concentrations, their frequency of occurrence, and effect concentrations, insecticides posed higher risks to aquatic organisms and human health than either fungicides or herbicides. Total pesticide concentrations were significantly positively correlated with suspended particulate matter, dissolved organic carbon, soil pH, normalized difference vegetation index, adjacent cropland area; and were negatively associated with adjacent grassland area. The cropland area largely influences pesticide distribution in the surface water of the Jiaodong Peninsula.

2.
Vox Sang ; 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36654528

RESUMO

BACKGROUND AND OBJECTIVES: Transfusion-associated circulatory overload (TACO) and transfusion-related acute lung injury (TRALI) are serious adverse transfusion reactions. Standardized surveillance definitions are important to ensure consistent reporting of cases. Recently, revised definitions have been developed for TACO and TRALI, the latter of which has not yet been widely implemented. This study aimed to assess the impact of the new TACO and TRALI definitions on haemovigilance reporting. MATERIALS AND METHODS: The Australian Red Cross Lifeblood Adverse Transfusion Reaction database was accessed to identify all cases of suspected or confirmed TACO and TRALI referred from 1 July 2015 to 30 June 2019. Cases were assessed against both the former and new definitions and the results were compared. RESULTS: A total of 73 cases were assessed. There were 48 TACO cases identified. Only 26 of 48 cases strictly met the former 2011 International Society of Blood Transfusion (ISBT) definition of TACO; 6 cases did not meet the definition and 16 cases lacked sufficient clinical details. In comparison, 46 cases met the revised 2018 ISBT definition, with only 2 cases having insufficient details. There were 24 cases of TRALI according to the existing 2004 Canadian Consensus Conference (CCC) definition compared with 25 cases according to the proposed 2019 revised definition. CONCLUSION: The revised TACO definition captured more cases than the former definition. No significant differences were observed in the number of TRALI cases under the proposed new definition. This is the first study to provide validation data for the revised TRALI definition.

3.
Proteomics ; : e2200432, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36625413

RESUMO

Hepatocellular carcinoma (HCC), the most common type of primary liver cancer, often metastasizes to the lungs. The implications of lysine lactylation (Kla), a recently identified histone post-translational modification (PTM), in the pathology of HCC remain unclear. Here, we report the first proteomic survey of this specific modification in HCC (with no metastasis during 3 years of follow-up), normal liver tissues, and lung metastasis samples of HCC. Of the 2045 modification sites detected on 960 proteins, 1438 sites on 772 proteins contained quantitative information. Subsequently, we analyzed the differentially modified proteins among the different groups. Differentially lactylated proteins were found to be involved in several biological processes, including-but not limited to-amino acid metabolism, ribosomal protein synthesis, and fatty acid metabolism. In addition, we identified numerous highly valuable lactate-modified proteins from the literature. Among them, we verified the lactate modification levels of the following two tumor-related proteins and obtained similar results: USP14 and ABCF1. These two modified proteins will be further investigated in our future studies. This paper is the first report on the lactylome of HCC and it provides a reliable foundation for further research on Kla in HCC.

4.
Stem Cell Res ; 66: 102993, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36502622

RESUMO

Schizophrenia (SCZ) and obsessive-compulsive disorder (OCD) are complex polygenic disorders with brain morphology abnormalities. The etiologies and relationship of both disorders remain elusive, and should be further investigated. Thus, induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) from an OCD patient, his mother with SCZ and his healthy father with reprograming method. All iPSCs were characterized to have normal karyotype and expression of pluripotency makers. These iPSCs will be a valuable model to elucidate the pathophysiological mechanisms and association of both diseases.


Assuntos
Células-Tronco Pluripotentes Induzidas , Transtorno Obsessivo-Compulsivo , Esquizofrenia , Feminino , Humanos , Masculino , Esquizofrenia/genética , Esquizofrenia/metabolismo , Mães , Células-Tronco Pluripotentes Induzidas/metabolismo , Leucócitos Mononucleares , Transtorno Obsessivo-Compulsivo/genética , Transtorno Obsessivo-Compulsivo/metabolismo , Pai
5.
Mol Plant Microbe Interact ; : MPMI09220182R, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36515967

RESUMO

Most bacteria use type II fatty acid synthesis (FAS) systems for synthesizing fatty acids, of which the conserved FabA-FabB pathway is considered to be crucial for unsaturated fatty acid (UFA) synthesis in gram-negative bacteria. Xanthomonas campestris pv. campestris, the phytopathogen of black rot disease in crucifers, produces higher quantities of UFAs under low-temperature conditions for increasing membrane fluidity. The fabA and fabB genes were identified in the X. campestris pv. campestris genome by BLAST analysis; however, the growth of the X. campestris pv. campestris fabA and fabB deletion mutants was comparable to that of the wild-type strain in nutrient and minimal media. The X. campestris pv. campestris ΔfabA and ΔfabB strains produced large quantities of UFAs and, altogether, these results indicated that the FabA-FabB pathway is not essential for growth or UFA synthesis in X. campestris pv. campestris. We also observed that the expression of X. campestris pv. campestris fabA and fabB restored the growth of the temperature-sensitive Escherichia coli fabA and fabB mutants CL104 and CY242, respectively, under non-permissive conditions. The in-vitro assays demonstrated that the FabA and FabB proteins of X. campestris pv. campestris catalyzed FAS. Our study also demonstrated that the production of diffusible signal factor family signals that mediate quorum sensing was higher in the X. campestris pv. campestris ΔfabA and ΔfabB strains and greatly reduced in the complementary strains, which exhibited reduced swimming motility and attenuated host-plant pathogenicity. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.

6.
Redox Biol ; 59: 102582, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36584600

RESUMO

Obeticholic acid (OCA) has been examined to treat non-alcoholic steatohepatitis (NASH), but has unsatisfactory antifibrotic effect and deficient responsive rate in recent phase III clinical trial. Using a prolonged western diet-feeding murine NASH model, we show that OCA-shaped gut microbiota induces lipid peroxidation and impairs its anti-fibrotic effect. Mechanically, Bacteroides enriched by OCA deconjugates tauro-conjugated bile acids to generate excessive chenodeoxycholic acid (CDCA), resulting in liver ROS accumulation. We further elucidate that OCA reduces triglycerides containing polyunsaturated fatty acid (PUFA-TGs) levels, whereas elevates free PUFAs and phosphatidylethanolamines containing PUFA (PUFA-PEs), which are susceptible to be oxidized to lipid peroxides (notably arachidonic acid (ARA)-derived 12-HHTrE), inducing hepatocyte ferroptosis and activating hepatic stellate cells (HSCs). Inhibiting lipid peroxidation with pentoxifylline (PTX) rescues anti-fibrotic effect of OCA, suggesting combination of OCA and lipid peroxidation inhibitor could be a potential antifibrotic pharmacological approach in clinical NASH-fibrosis.


Assuntos
Microbiota , Hepatopatia Gordurosa não Alcoólica , Camundongos , Animais , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/metabolismo , Peroxidação de Lipídeos , Fígado/metabolismo , Ácido Quenodesoxicólico/farmacologia , Ácido Quenodesoxicólico/metabolismo , Ácido Quenodesoxicólico/uso terapêutico
7.
BMC Geriatr ; 22(1): 847, 2022 11 11.
Artigo em Inglês | MEDLINE | ID: mdl-36368951

RESUMO

BACKGROUND: We have witnessed frailty, which characterized by a decline in physiological reserves, become a major public health issue in older adults. Understanding the influential factors associated with frailty may help prevent or if possible reverse frailty. The present study aimed to investigate factors associated with frailty status and frailty transition in a community-dwelling older population. METHODS: A prospective cohort study on community-dwelling subjects aged ≥ 60 years was conducted, which was registered beforehand (ChiCTR 2,000,032,949). Participants who had completed two visits during 2020-2021 were included. Frailty status was evaluated using the Fried frailty phenotype. The least absolute shrinkage and selection operator (LASSO) regression was applied for variable selection. Bayesian network analysis with the max-min hill-climbing (MMHC) algorithm was used to identify factors related to frailty status and frailty transition. RESULTS: Of 1,981 subjects at baseline, 1,040 (52.5%) and 165 (8.33%) were classified as prefrailty and frailty. After one year, improved, stable, and worsening frailty status was observed in 460 (35.6%), 526 (40.7%), and 306 (23.7%) subjects, respectively. Based on the variables screened by LASSO regression, the Bayesian network structure suggested that age, nutritional status, instrumental activities of daily living (IADL), balance capacity, and social support were directly related to frailty status. The probability of developing frailty is 14.4% in an individual aged ≥ 71 years, which increases to 20.2% and 53.2% if the individual has balance impairment alone, or combined with IADL disability and malnutrition. At a longitudinal level, ADL/IADL decline was a direct predictor of worsening in frailty state, which further increased the risk of hospitalization. Low high-density lipoprotein cholesterol (HDL-C) and diastolic blood pressure (DBP) levels were related to malnutrition, and further had impacts on ADL/IADL decline, and ultimately led to the worsening of the frailty state. Knowing the status of any one or more of these factors can be used to infer the risk of frailty based on conditional probabilities. CONCLUSION: Older age, malnutrition, IADL disability, and balance impairment are important factors for identifying frailty. Malnutrition and ADL/IADL decline further predict worsening of the frailty state.


Assuntos
Fragilidade , Desnutrição , Humanos , Idoso , Fragilidade/diagnóstico , Fragilidade/epidemiologia , Fragilidade/complicações , Vida Independente , Idoso Fragilizado , Atividades Cotidianas , Avaliação Geriátrica , Teorema de Bayes , Estudos Prospectivos , Desnutrição/complicações
8.
J Med Chem ; 65(23): 15749-15769, 2022 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-36414390

RESUMO

Pharmacological targeting cancer stem cells are emerging as a novel therapeutic modality for cancer treatment and prevention. Human cytochrome P450 enzyme CYP4Z1 represents a promising target for its potential role in attenuating the stemness of breast cancer cells. In order to develop potent and selective CYP4Z1 inhibitors, a series of novel N-hydroxyphenylformamidines were rationally designed and synthesized from a pan-CYP inhibitor HET0016. CYP4Z1 inhibitory activities of the newly synthesized derivatives were evaluated, and the structure-activity relationships (SARs) were summarized. Among them, compound 7c exhibited the best inhibitory activity with an IC50 value of 41.8 nM. Furthermore, it was found that 7c decreased the expression of stemness markers, spheroid formation, and metastatic ability as well as tumor-initiation capability in a concentration-dependent manner in vitro and in vivo. Altogether, compound 7c might be a potential lead compound to develop CYP4Z1 inhibitor with more favorable druggability for clinical application to treat breast cancer.

9.
Artigo em Inglês | MEDLINE | ID: mdl-36429858

RESUMO

Parental burnout refers to the feelings of extreme exhaustion that many parents experience due to parenting. Although parental burnout has received considerable interest from researchers, the effects and mechanisms of parental burnout on adolescents' physical and psychological development remain largely unknown. This study investigated the relationship between parental burnout and adolescents' problem behaviors in Chinese families. We explored the mediating roles of family function, parental psychological aggression, and adolescent self-control, as well as the moderating role of co-parenting. Questionnaires were distributed to 600 adolescents and their primary caregivers, and the data were collected on three different occasions. In total, 174 paired participants completed the survey (44.9% boys; Mean-age = 14.18 years). Bootstrapping results showed that parental burnout was positively associated with adolescents' problem behavior. In addition, family function, parental psychological aggression, and adolescent self-control mediated the relationship between parental burnout and adolescents' problem behavior. Co-parenting moderated the effects of parental burnout on family function and parental psychological aggression. The result of structural equation modeling (SEM) generally supported the above results. These findings reveal the negative effects and mechanisms of parental burnout on adolescent problem behavior, providing empirical evidence that can be applied to prevent the negative effects of parental burnout.


Assuntos
Comportamento do Adolescente , Comportamento Problema , Masculino , Adolescente , Humanos , Feminino , Esgotamento Psicológico/epidemiologia , Agressão , Poder Familiar
11.
Crit Care ; 26(1): 279, 2022 09 16.
Artigo em Inglês | MEDLINE | ID: mdl-36114552

RESUMO

OBJECTIVE: The aim of this study was to evaluate the efficacy and safety of remimazolam besylate compared with propofol in maintaining mild-to-moderate sedation in patients receiving long-term mechanical ventilation. METHODS: In this single-centered randomized pilot study, adult patients mechanically ventilated longer than 24 h were randomized to receive remimazolam besylate or propofol. The target sedation range was - 3 to 0 on the Richmond Agitation and Sedation Scale (RASS). The primary outcome was the percentage of time in the target sedation range without rescue sedation. The secondary outcomes were ventilator-free days at day 7, the length of ICU stay and 28-day mortality. RESULTS: Thirty patients were assigned to each group. No difference was identified between the remimazolam group and propofol group in median age [60.0 (IQR, 51.5-66.3) years vs. 64.0 (IQR, 55.0-69.3) years, respectively, p = 0.437] or the median duration of study drug infusion [55.0 (IQR, 28.3-102.0) hours vs. 41.0 (IQR, 24.8-74.3) hours, respectively, p = 0.255]. The median percentage of time in the target RASS range without rescue sedation was similar in remimazolam and propofol groups [73.2% (IQR, 41.5-97.3%) vs. 82.8% (IQR, 65.6-100%), p = 0.269]. No differences were identified between the two groups in terms of ventilator-free days at day 7, length of ICU stay, 28-day mortality or adverse events. CONCLUSIONS: This pilot study suggested that remimazolam besylate was effective and safe for long-term sedation in mechanically ventilated patients compared with propofol.


Assuntos
Propofol , Adulto , Idoso , Benzodiazepinas , Humanos , Hipnóticos e Sedativos/farmacologia , Hipnóticos e Sedativos/uso terapêutico , Pessoa de Meia-Idade , Projetos Piloto , Propofol/efeitos adversos , Respiração Artificial
12.
Geriatr Gerontol Int ; 22(9): 759-765, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36058626

RESUMO

AIM: The evaluations of handgrip strength (HGS) weakness and asymmetry have implications for the comprehensive geriatric assessment. The aim of this study was to investigate the association of HGS weakness and asymmetry on cardiovascular outcomes in older outpatients. METHODS: This was a prospective observational cohort study of 364 Geriatrics outpatients aged ≥60 years, in which all participants carried out HGS tests at baseline. Patients with HGS <28 kg for men and <18 kg for women were diagnosed as HGS weakness, and HGS ratio <0.90 or >1.10 were diagnosed as HGS asymmetry. Primary outcomes defined as the major adverse cardiovascular event and composite end-points were assessed during a 21-month median follow-up. RESULTS: Among 364 participants, 155 (42.6%) showed HGS weakness, and 160 (44.0%) showed HGS asymmetry. HGS weakness was associated with major adverse cardiovascular events (HR 2.76, 95% CI 1.22-6.27) and composite end-points (HR 2.84, 95% CI 1.40-5.77). However, no significant correlation between HGS asymmetry and cardiovascular outcomes was observed. Compared with the normal and symmetric HGS group, older adults with HGS weakness and asymmetry together had a higher risk of major adverse cardiovascular events (HR 5.23, 95% CI 1.56-17.54) and composite end-points (HR 4.00, 95% CI 1.56-10.28). CONCLUSIONS: HGS weakness and asymmetry together might increase the risk of cardiovascular outcomes in older outpatients. HGS asymmetry offers complementary information to HGS weakness when making a comprehensive assessment of HGS. Geriatr Gerontol Int 2022; 22: 759-765.


Assuntos
Doenças Cardiovasculares , Força da Mão , Idoso , Doenças Cardiovasculares/complicações , Doenças Cardiovasculares/diagnóstico , Doenças Cardiovasculares/epidemiologia , Feminino , Avaliação Geriátrica , Humanos , Masculino , Pacientes Ambulatoriais , Estudos Prospectivos
13.
Nutr Rev ; 2022 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-36130411

RESUMO

Cellular activities such as DNA synthesis, adenosine triphosphate production, and mitochondrial respiration are affected by iron metabolism. Disturbance of iron homeostasis usually leads to damage in cells and organs in the context of iron overload or deficiency. Thus, iron, a key regulator in nutritional immunity, was shown to be critical in innate and adaptive immunity. Unlike apoptosis, ferroptosis, a feature of iron-dependent lipid peroxidation, is thought to be associated with immune regulation because of its immunogenic nature. In this review, we summarize the role of iron and ferroptosis in immune regulation and discuss their therapeutic potential in the treatment of arthropathies like osteoarthritis and rheumatoid arthritis.

15.
BMC Public Health ; 22(1): 1414, 2022 07 26.
Artigo em Inglês | MEDLINE | ID: mdl-35879692

RESUMO

BACKGROUND: While physical inactivity or prolonged sitting has been linked to an increased risk of frailty, the interaction between sitting time (ST), physical activity (PA) and frailty is not well understood. The aim of this study was to examine the dose-response relationship between PA, ST and frailty and further to evaluate the interaction effect of PA and ST on frailty in the context of regular COVID-19 epidemic prevention and control in China. METHODS: A cross-sectional analysis was performed on 1458 participants (age ≥ 60) enrolled from a prospective cohort study of frailty in elderly people of Fujian Province. PA and ST levels were assessed using the International Physical Activity Questionnaire. A 40-item frailty index (FI) quantified frailty. Multivariable logistic regression and linear regression models were applied to examine the dose-response relationship between PA or ST and frailty level. Interaction plots were used to visualise the interaction effects of PA and ST on frailty. RESULTS: Compared with light PA, the odds ratios (ORs) for frailty were significantly lower for moderate PA (OR, 0.609 [95% CI, 0.419, 0.885], P < .001) and vigorous PA (OR, 0.399 [95% CI, 0.236,0.673], P < .001). Comparing subjects with ST <  4 h/day, those with ST ≥ 8 h/day were significantly more likely to be diagnosed with frailty (OR, 3.140 [95% CI, 1.932, 5.106], P < .001), 6-8 h/day (OR, 1.289 [95% CI, 0.835, 1.989], P >0.05), and 4-6 h/day (OR, 1.400 [95% CI, 0.972, 2.018], P >0.05). Each one unit increase in metabolic equivalents (h/day) of PA was related to an average 0.928 (0.887, 0.971) decrease in prevalence of frailty, while each one unit increase in sitting time (h/day) was related to average 1.114 (1.046,1.185) increase in prevalence of frailty. Negative interactive effects of PA and ST on frailty were observed (P < 0.001). CONCLUSION: There are nonlinear and linear dose-response relationships between PA, SB and frailty respectively. In addition, excess ST may counteract the beneficial effects of PA on frailty. Interventions that focus on reducing excess ST may be effective strategies to reduce the risk of frailty and should be taken seriously by public health authorities, especially in the context of regular epidemic prevention and control in China.


Assuntos
COVID-19 , Fragilidade , Idoso , COVID-19/epidemiologia , COVID-19/prevenção & controle , Estudos Transversais , Exercício Físico/fisiologia , Fragilidade/epidemiologia , Humanos , Vida Independente , Estudos Prospectivos , Comportamento Sedentário
16.
Int Immunopharmacol ; 110: 109025, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35853280

RESUMO

BACKGROUND: Recent studies have shown that microRNAs (miRNAs) are aberrantly expressed in patients with Crohn's disease (CD). This suggests that the aberrant expression of miRNAs may contribute to the development of CD. Currently, the specific miRNAs involved in CD development have not been clearly identified. Therefore, we aimed to identify CD-associated miRNAs and explore their functions. METHODS: miRNA microarray analysis was performed to screen for differentially expressed miRNAs in colon tissues from normal controls (NC) and CD patients. The identified miRNAs were validated using quantitative real-time PCR (qPCR). The therapeutic roles of miR-20a-5p mimics via the delivery of poly(lactic-co-glycolic acid) microspheres (PLGA MSs) were further investigated in IL-10-/- mice with spontaneous chronic colitis that were used as a model of CD. The target genes of miR-20a-5p and the associated signaling pathways were identified through bioinformatic analysis and experimental verification of the interactions between the targets predicted by the algorithms and dysregulated mRNAs. RESULTS: The analysis showed that miR-20a-5p was the most significantly downregulated miRNA in patients with CD. Treatment with PLGA MSs carrying miR-20a-5p significantly ameliorated the colitis, decreased mucosal inflammation, and improved epithelial barrier function. Bioinformatic analysis and experimental studies showed that miR-20a-5p inhibition enhanced Th17 differentiation and improved intestinal epithelial barrier function by targeting STAT3. CONCLUSIONS: Downregulation of miR-20a-5p improved the intestinal epithelial barrier function and prevented CD development through the STAT3/IL-17 signaling pathway. Therefore, the delivery of miR-20a-5p by PLGA MSs may serve as a potential therapeutic strategy for CD treatment.


Assuntos
Colite , Doença de Crohn , MicroRNAs , Animais , Colite/induzido quimicamente , Colite/genética , Colite/terapia , Doença de Crohn/genética , Doença de Crohn/metabolismo , Doença de Crohn/terapia , Regulação para Baixo , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , Microesferas
17.
J Colloid Interface Sci ; 626: 283-295, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-35797870

RESUMO

Electrocatalytic filtration process adopting the electrocatalytic membrane as both filtration membrane and active electrode showed great prospect on the organic pollutant removal from water. In this work, a high-performance metal-free polypyrrole (PPy) coated carbon-based electrocatalytic membrane (PPy@CCM) was developed through the facile and controllable electro-polymerization deposition method. Structural properties and electrochemical performance of the prepared PPy@CCM were characterized systematically. The influences of preparation parameters and operational parameters on water treatment performance of PPy@CCM were also investigated. Results indicates that the spherical PPy particles uniformly distributed on the surface of PPy@CCM. Coating with PPy particles can significantly improve the hydrophilicity and electrochemical activity of CCM, therefore PPy@CCM has lower hydraulic resistance and higher water treatment performance than CCM. The phenol and chemical oxygen demand (COD) removal rates obtained by PPy@CCM are up to 99.51% and 89.90%, respectively, under the optimal condition of 2.0 V cell potential, 2.50 g·L-1 Na2SO4, 1.5 ml·min-1 flow rate and 50 mg·L-1 phenol, and only 0.5 kWh·kgCOD-1 energy consumption is consumed. In addition, PPy@CCM also exhibits good treatment performance in different water matrixes. Moreover, PPy@CCM has good stability for several cycle operation and considerable applicability for different types of organic pollutants removal. The oxidation mechanism study reveals that PPy@CCM has both direct and indirect oxidation activity during the electrocatalytic filtration treatment, and the coating of PPy can improve the direct oxidation ability and ·OH yield of CCM.


Assuntos
Polímeros , Pirróis , Carbono , Fenóis , Polímeros/química , Pirróis/química
18.
Clin Interv Aging ; 17: 1057-1068, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35832608

RESUMO

Purpose: Intrinsic capacity (IC) reflects the overall health status of older adults and has great public health significance. But few studies described the related biomarkers for IC. The aim of this study was to investigate the association between homocysteine (Hcy) and IC in older adults. Participants and Methods: This cross-sectional study included 1927 community-dwelling Chinese older adults aged 60-98 years from May 2020 to December 2020. Data were collected through interviews, physical examinations, and laboratory tests. IC involved five domains of cognition, locomotion, sensory, vitality, and psychology evaluated by the Mini-cog scale, 4-m walk test, self-reported visual and hearing conditions, MNA-SF scale, and GDS-4 scale, respectively. The score of each domain dichotomized as 0 (normal) and 1 (impaired) was added together to an IC total score. Low IC was defined as a score of 3-5, and high IC as 0-2. Hcy was measured by a two-reagent enzymatic assay. A restricted cubic spline regression model was used to explore the non-linear relationship between Hcy and low IC. Results: Hcy was higher in the low IC group than in the high IC group. Restricted cubic spline analysis revealed a J-shaped nonlinear association between Hcy and low IC. The risk of IC decline was slowly decreased until 8.53 µmol/L of Hcy (OR=0.753, 95% CI=0.520-1.091, P=0.132), and increased with elevations of per 5 µmol/L Hcy afterwards (OR=1.176, 95% CI=1.059-1.327, P=0.005). Among the five domains of IC, Hcy had ORs of 1.116 (1.009-1.247) for cognition impairment, 1.167 (1.055-1.305) for vitality, and 1.160 (1.034-1.303) for psychology per 5 µmol/L increase in Hcy above the change point. Additional sensitivity analysis also demonstrated the nonlinear association between Hcy and low IC. Conclusion: Hcy had a J-shaped association with low IC. Higher Hcy (Hcy ≥8.53µmol/L) might provide clinical implications for early identifying the risk of low IC.


Assuntos
Disfunção Cognitiva , Vida Independente , Idoso , China , Estudos Transversais , Homocisteína , Humanos
19.
Microbiol Spectr ; 10(3): e0004722, 2022 06 29.
Artigo em Inglês | MEDLINE | ID: mdl-35647690

RESUMO

Nonalcoholic fatty liver disease (NAFLD) is a prevalent and progressive disease spectrum ranging from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH), yet there is no effective treatment and efficient noninvasive diagnostic method for NASH. The present study investigated the longitudinal alternations of gut microbiota in the Western diet (WD) induced murine NAFLD model using 16S rRNA sequencing. Evident steatosis and inflammation were detected in the liver at the 8th and 12th week, while prompted hepatic oxidative injury and fibrosis were found at the 16th week. In this progressive process, impaired bile acid (BA) metabolism plays a vital part. Long-term WD intervention alters microbial richness and composition in the intestine, shaping characteristic microbial feature correspondence to each NAFLD stage. Descending abundances of Clostridia and Ruminococcaceae were found in NAFLD progression, while inflammation-related microbes [Eubacterium]_fissicatena_group, Romboutsia, and Erysipelatoclostridium were verified to identify borderline NASH at 8th and 12th week, and BA-associated taxa Dubosiella, Bosea, Helicobacter, and Alistipes were recognized as special symbols reflecting the state of oxidative damage and fibrosis in NASH at 16th week. Further, feces and colon abundances of Akkermansia were verified to be depleted in the process of borderline NASH progressed to NASH, and exhibited substantial correlations with NAFLD indexes ALT, AST, TC, and TBA. These characteristic taxa were effective to identify NAFLD and NASH, and microbiota-derived predictive models for NAFLD and NASH exhibited great potential (AUC 0.983 and 0.784). These findings demonstrate that a core set of gut microbiome especially BA-related taxa may be adopted as a noninvasive diagnostic tool for NAFLD and NASH. IMPORTANCE This study concentrates on longitudinal alternations of gut microbiota in NAFLD progression and discovers the interrelationships between them. These findings may uncover the role of gut microbiota in NAFLD progression and identify novel noninvasive diagnostic tools for NAFLD based on microbial biomarkers.


Assuntos
Microbioma Gastrointestinal , Hepatopatia Gordurosa não Alcoólica , Animais , Fibrose , Inflamação/metabolismo , Fígado/metabolismo , Camundongos , Hepatopatia Gordurosa não Alcoólica/microbiologia , RNA Ribossômico 16S/genética
20.
Comput Intell Neurosci ; 2022: 4077516, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35669663

RESUMO

This study investigates the mechanism of digital linguistic landscapes in enabling engineering education for smart construction according to the educational dimensions of A (ability), S (skill), and K (knowledge). A questionnaire survey was conducted based on the core concepts of the informative dimension and symbolic dimension in digital language landscape as well as the ability dimension, knowledge dimension, and skill dimension in engineering education. Structural equation modeling (SEM) was used as the test method. The results of the research demonstrate that the informative dimension and symbolic dimension are two main aspects of DLL in education of engineering students for smart construction. Additionally, DLL has a significant positive impact on the ability, knowledge, and skill education of engineering students for smart construction. The research has theoretical and practical significance, as it not only enriches research on the relationship between DLL and engineering education for smart construction but also expands the theoretical understanding of engineering education from the perspective of linguistics. Furthermore, the study explores the path of the practical application of digital language landscape to engineering education for smart construction.


Assuntos
Linguística , Estudantes , Engenharia , Humanos , Aprendizagem
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