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1.
Front Psychol ; 13: 872301, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36081716

RESUMO

Objective: The study aims to discuss the longitudinal impact of the parent-teacher relationship on students' academic achievements in China. Method: Based on the China Education Panel Survey, covering the data from 438 classes of 112 schools in 28 county-level administrative areas in China, we used the hierarchical linear model to analyze the data. Results: We found that the parents' active communication with teachers, parents' participation in parent meetings, teachers' active contact, whether parents are afraid to communicate with teachers, and parents' willingness to participate in parent meetings have significant relationships with students' academic achievements. At the class level, the extent of teachers' stress from parents' requests and teachers' perception of respect from parents also affected students' academic achievements significantly in the Chinese context. Conclusion: There was a longitudinal association between the parent-teacher relationship and students' academic achievements. The practical implication was discussed in the paper.

2.
ChemSusChem ; 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36094056

RESUMO

Lignin-based activated carbon (LAC) is a promising high-quality functional material due to high surface area, abundant porous structure, and various functional groups. Modification is the most important step to functionalize LAC by altering its porous and chemical properties. Herein, this review summarizes the state-of-the-art modification technologies of LAC toward advanced applications. Promising modification approaches are reviewed to display their effects on the preparation of LAC. The multiscale changes in the porosity and the surface chemistry of LAC are fully discussed. Advanced applications are then introduced to show the potential of LAC for supercapacitor electrode, catalyst support, hydrogen storage and carbon dioxide capture. Finally, the mechanistic structure-function relationships of LAC are elaborated. These results highlight that modification technologies play a special role in altering the properties and defining the functionalities of LAC, which could be a promising porous carbon material toward industrial applications.

3.
Chin Med J (Engl) ; 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36070466

RESUMO

BACKGROUND: The fetal growth charts in widest use in China were published by Hadlock >35 years ago and were established on several hundred of American pregnant women. After that, >100 fetal growth charts were published around the world. We attempted to assess the impact of applying the long-standing Hadlock charts and other charts in a Chinese population and to compare their ability to predict newborn small for gestational age (SGA). METHODS: For this retrospective observational study, we reviewed all pregnant women (n = 106,455) who booked prenatal care with ultrasound measurements for fetal biometry at the Shenzhen Maternity and Child Healthcare Hospital between 2012 and 2019. A fractional polynomial regression model was applied to generate Shenzhen fetal growth chart ranges for head circumference (HC), biparietal diameter, abdominal circumference (AC), and femur length (FL). The differences between Shenzhen charts and published charts were quantified by calculating the Z-score. The impact of applying these published charts was quantified by calculating the proportions of fetuses with biometric measurements below the 3rd centile of these charts. The sensitivity and area under the receiver operating characteristic curves of published charts to predict neonatal SGA (birthweight <10th centile) were assessed. RESULTS: Following selection, 169,980 scans of fetal biometry contributed by 41,032 pregnancies with reliable gestational age were analyzed. When using Hadlock references (<3rd centile), the proportions of small heads and short femurs were as high as 8.9% and 6.6% in late gestation, respectively. The INTERGROWTH-21st standards matched those of our observed curves better than other charts, in particular for fat-free biometry (HC and FL). When using AC<10th centile, all of these references were poor at predicting neonatal SGA. CONCLUSIONS: Applying long-standing Hadlock references could misclassify a large proportion of fetuses as SGA. INTERGROWTH-21st standard appears to be a safe option in China. For fat-based biometry, AC, a reference based on the Chinese population is needed. In addition, when applying published charts, particular care should be taken due to the discrepancy of measurement methods.

5.
Nanoscale Adv ; 4(9): 2224-2232, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-36133451

RESUMO

In this research, rare earth nanoparticles coupled with dihydroartemisinin (DHA) and a targeted antibody (RENP-DHA-Cap) for sprayed NIR II imaging and photodynamic therapy (PDT) of tongue cancer were designed. Genetic algorithms combined with combinatorial chemistry were proposed and successfully achieved in a single optimized luminescent phosphor with enhanced NIR II and high upconversion luminescence (UCL) under a NIR laser of wavelength 980 nm or/and 808 nm. In particular, T1 magnetic resonance imaging (MRI) signals can be adjusted with the Gd ion concentration. In combination with the targeted antibody of capmatinib (Cap), precise NIR II imaging for in situ tongue cancer by a simple spray method can be achieved. Most importantly, NIR II imaging and PDT treatment can be realized with RENP-DHA-capmatinib injected intravenously. This orthogonal theranostic mode with precise diagnosis under 808 nm and targeted effective treatment under 980 nm may promote tongue cancer theranostics.

6.
Curr Oncol ; 29(9): 6303-6313, 2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36135064

RESUMO

Background: The PREDICT-HN study aimed to systematically assess the kinetics of imaging MR biomarkers during head and neck radiotherapy. Methods: Patients with intact squamous cell carcinoma of the head and neck were enrolled. Pre-, during, and post-treatment MRI were obtained. Serial GTV and ADC measurements were recorded. The correlation between each feature and the GTV was calculated using Spearman's correlation coefficient. The linear mixed model was used to evaluate the change in GTV over time. Results: A total of 41 patients completed the study. The majority (76%) had oropharyngeal cancer. A total of 36 patients had intact primary tumours that can be assessed on MRI, and 31 patients had nodal disease with 46 nodes assessed. Median primary GTV (GTVp) size was 14.1cc. The rate of GTVp shrinkage was highest between pre-treatment and week 4. Patients with T3-T4 tumours had a 3.8-fold decrease in GTVp compared to T1-T2 tumours. The ADC values correlated with residual GTVp. The median nodal volume (GTVn) was 12.4cc. No clinical features were found to correlate with GTVn reduction. The overall change in ADC for GTVn from pre-treatment was significant for 35th-95th percentiles in weeks 1-4 (p < 0.001). Conclusion: A discrepancy in the trajectory of ADC between primary and nodal sites suggested that they exhibit different treatment responses and should be analysed separately in future studies.


Assuntos
Carcinoma de Células Escamosas , Neoplasias de Cabeça e Pescoço , Biomarcadores , Carcinoma de Células Escamosas/patologia , Imagem de Difusão por Ressonância Magnética/métodos , Neoplasias de Cabeça e Pescoço/diagnóstico por imagem , Neoplasias de Cabeça e Pescoço/radioterapia , Humanos , Imageamento por Ressonância Magnética
7.
J Hazard Mater ; 439: 129674, 2022 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-36104903

RESUMO

Antibiotic mixtures in the environment result in the development of bacterial strains with resistance against multiple antibiotics. Oxidases are versatile that can bio-remove antibiotics. Various laccases (LACs), manganese peroxidases (MNPs), and versatile peroxidase (VP) were reconstructed in Pichia pastoris. For the single antibiotics, over 95.0% sulfamethoxazole within 48 h, tetracycline, oxytetracycline, and norfloxacin within 96 h were bio-removed by recombinant VP with α-signal peptide, respectively. In a mixture of the four antibiotics, 80.2% tetracycline and 95.6% oxytetracycline were bio-removed by recombinant MNP2 with native signal peptide (NSP) within 8 h, whereas < 80.0% sulfamethoxazole was bio-removed within 72 h, indicating that signal peptides significantly impacted removal efficiencies of antibiotic mixtures. Regarding mediators for LACs, 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) resulted in better removal efficiencies of multi-antibiotic mixtures than 1-hydroxybenzotriazole or syringaldehyde. Furthermore, artificial microbial consortia (AMC) producing LAC2 and MNP2 with NSP significantly improved bio-removal efficiency of sulfamethoxazole (95.5%) in four-antibiotic mixtures within 48 h. Tetracycline and oxytetracycline were completely bio-removed by AMC within 48 and 72 h, respectively, indicating that AMC accelerated sulfamethoxazole, tetracycline, and oxytetracycline bio-removals. Additionally, transformation pathways of each antibiotic by recombinant oxidases were proposed. Taken together, this work provides a new strategy to simultaneously remove antibiotic mixtures by AMC.


Assuntos
Antibacterianos , Oxitetraciclina , Antibacterianos/metabolismo , Biotransformação , Lacase/metabolismo , Consórcios Microbianos , Sinais Direcionadores de Proteínas , Sulfametoxazol , Tetraciclina
8.
Med Mycol ; 60(9)2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36055797

RESUMO

Baicalein could inhibit the growth and biofilm formation of Candida albicans, the most common clinical fungal pathogen. However, the antifungal mechanism of baicalein has not been elucidated. In this study, isobaric tags for relative and absolute quantification (iTRAQ) was used to verify the mechanism of antifungal fluconazole and baicalein. A total of 58 common proteins were detected in cells treated with fluconazole. These proteins encompassed fluconazole-targeted sterol synthesis pathway, including Erg11p, Erg6p, Erg3p, Erg25p, Erg5p, Erg10p, and Ncp1p. Next, iTRAQ was applied to the comparison of baicalein-treated C. albicans proteins, which detected 16 common proteins. The putative NADH dehydrogenase Cpd2p and the ATP-binding cassette transporter Snq2p were the most upregulated proteins with the treatment of baicalein. Our results showed that CPD2 disruption elevated C. albicans resistance to baicalein significantly both in vitro and in vivo. Further in-depth studies revealed that CPD2 disruption reduced the activation of C. albicans metacaspase and partially restored the mitochondrial membrane potential reduction caused by the treatment of baicalein, which indicated that CPD2 was involved in the apoptosis induced by baicalein. Consistently, under the treatment of baicalein, CPD2Δ/Δ mutant produced lower reactive oxygen species that was critical in causing oxidative damage and apoptosis in C. albicans. These results indicated that baicalein could increase intracellular oxidative damage by upregulating the expression of Cpd2p so as to inhibit the growth of C. albicans, which provides new insights for investigating the antifungal target of baicalein.


In our study, isobaric tags for relative and absolute quantification (iTRAQ) was used to study the antifungal mechanisms of fluconazole and baicalein. Baicalein could enhance the oxidative stress of Candida albicans by upregulating CPD2 expression.


Assuntos
Candida albicans , Fluconazol , Animais , Antifúngicos/metabolismo , Antifúngicos/farmacologia , Farmacorresistência Fúngica , Flavanonas , Fluconazol/farmacologia , Testes de Sensibilidade Microbiana/veterinária , Estresse Oxidativo , Proteômica
9.
Artigo em Inglês | MEDLINE | ID: mdl-36112290

RESUMO

The migration and transformation of dissolved organic matter (DOM) caused by landfill leachate leakage affected the phylogenetic development of bacterial communities in groundwater around the landfill. Previous studies mainly focused on the hydrochemical properties of DOM in groundwater contaminated by landfill leachate and the relationships between groundwater quality parameters and bacterial communities. However, the changes in DOM components and bacterial communities caused by landfill leachate leakage and their correlations remained unclear. In this work, we analyzed the evolution characteristics of DOM and identified the bacterial communities and their corresponding functions in groundwater around the landfill. The results showed that DOM content in groundwater increased after the diffusion of landfill leachate to groundwater. Significant differences in characteristics between DOM components were presented at different locations in the landfill leachate plume due to the physical dilution and bacterial degradation of DOM. One of the obvious manifestations was the tendency of humic acid-like substances to accumulate at downstream points. Samples from the contaminated aquifer had higher diversity and abundance of bacterial communities than those in the uncontaminated aquifer. Anaerobic or facultative anaerobic bacteria played predominant roles in contaminated groundwater, due to the input of organic matter, nitrate, and ammonia nitrogen. Redundancy analysis indicated that the content of fulvic acid-like DOM had a conspicuous impact on the composition of bacterial communities in the polluted groundwater. Vogesella were the dominant bacteria at the genus level in groundwater around the landfill. Furthermore, Vogesella were significant for microbial utilization and played an important role in the production of fulvic acid-like DOM. These results indicated that landfill leachate pollution posed a potential threat to the structure and function of bacterial communities in groundwater, and provided a basis for exploring the interaction between DOM composition and bacterial communities in groundwater plume contaminated by landfill leachate.

10.
ACS Infect Dis ; 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36048501

RESUMO

ATP provides energy in the biosynthesis of cellular metabolites as well as regulates protein functions through phosphorylation. Many ATP-dependent enzymes are antibacterial and anticancer targets including human kinases acted on by most of the successful drugs. In search of new chemotherapeutics for tuberculosis (TB), we screened repurposing compounds against the essential glutamine synthase (GlnA1) of Mycobacterium tuberculosis (Mtb) and identified linsitinib, a clinical-stage drug originally targeting kinase IGF1R/IR as a potent GlnA1 inhibitor. Linsitinib has direct antimycobacterial activity. Biochemical, molecular modeling, and target engagement analyses revealed the inhibition is ATP-competitive and specific in Mtb. Linsitinib also improves autophagy flux in both Mtb-infected and uninfected THP1 macrophages, as demonstrated by the decreased p-mTOR and p62 and the increased lipid-bound LC3B-II and autophagosome forming puncta. Linsitinib-mediated autophagy reduces intracellular growth of wild-type and isoniazid-resistant Mtb alone or in combination with bedaquiline. We have demonstrated that an IGF-IR/IR inhibitor can potentially be used to treat TB. Our study reinforces the concept of targeting ATP-dependent enzymes for novel anti-TB therapy.

11.
Nat Commun ; 13(1): 5361, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36097016

RESUMO

DNA data storage is a rapidly developing technology with great potential due to its high density, long-term durability, and low maintenance cost. The major technical challenges include various errors, such as strand breaks, rearrangements, and indels that frequently arise during DNA synthesis, amplification, sequencing, and preservation. In this study, a de novo strand assembly algorithm (DBGPS) is developed using de Bruijn graph and greedy path search to meet these challenges. DBGPS shows substantial advantages in handling DNA breaks, rearrangements, and indels. The robustness of DBGPS is demonstrated by accelerated aging, multiple independent data retrievals, deep error-prone PCR, and large-scale simulations. Remarkably, 6.8 MB of data is accurately recovered from a severely corrupted sample that has been treated at 70 °C for 70 days. With DBGPS, we are able to achieve a logical density of 1.30 bits/cycle and a physical density of 295 PB/g.


Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Armazenamento e Recuperação da Informação , Algoritmos , DNA/genética , Análise de Sequência de DNA
12.
Cancers (Basel) ; 14(15)2022 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-35954359

RESUMO

Recurrent microsatellite stable (MSS) endometrial cancer has poor response to conventional therapy and limited efficacy with immune checkpoint monotherapy. We conducted a retrospective study of recurrent MSS endometrial cancer patients enrolled in immunotherapy-based clinical trials at MD Anderson Cancer Center between 1 January 2010 and 31 December 2019. Patients were evaluated for radiologic response using RECIST 1.1 criteria, progression-free survival (PFS), and overall survival (OS). Thirty-five patients were treated with immune checkpoint inhibitors: 8 with monotherapy, 17 with immunotherapy (IO) in combination with another IO-only, and 10 with IO in combination with non-IO therapy. Among those treated with combination IO plus non-IO therapy, one had a partial response but 50% had clinical benefit. Patients who received combination IO plus non-IO therapy had improved PFS compared to those who received monotherapy (HR 0.56, 95% CI 0.33-0.97; p = 0.037) or combination IO-only therapy (HR 0.36, 95% CI 0.15-0.90; p = 0.028) and had improved OS when compared to monotherapy after adjusting for prior lines of therapy (HR 0.50, 95% CI 0.27-0.95; p = 0.036). The potential beneficial clinical outcomes of combination IO plus non-IO therapy in MSS endometrial cancer should be validated in a larger study.

13.
Int Immunopharmacol ; 111: 109103, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35944461

RESUMO

Neobavaisoflavone (Neo), as a traditional Chinese medicine, is the active ingredient in the herb Psoralea corylifolial and has antitumor activity. Myeloid-derived suppressor cells (MDSCs), which are a heterogeneous population of haematopoietic cells of the myeloid lineage, have been reported to be closely related to the pathogenesis of tumour progression, but whether Neo can regulate MDSC expansion and function remains unclear. Here, we found that Neo could inhibit the expansion and suppressive function of MDSCs by targeting STAT3. Importantly, Neo inhibited the growth of 4T1 and LLC tumours in vivo, as well as lung metastasis of 4T1 tumours in vivo. Furthermore, we identified MDSCs as the direct targets by which Neo attenuated tumour progression. In addition, Neo notably enhanced anti-PD-1 efficacy in anti-PD-1-insensitive 4T1 tumours. Therefore, our study sheds light on the development of Neobased therapeutic strategies against cancer.


Assuntos
Isoflavonas , Neoplasias Pulmonares , Células Supressoras Mieloides , Humanos , Terapia de Imunossupressão , Isoflavonas/farmacologia , Isoflavonas/uso terapêutico
14.
Artigo em Inglês | MEDLINE | ID: mdl-35946074

RESUMO

Our current knowledge of the mechanism between diabetes and cancer is limited. Oxidatively damaged nucleic acid is a critical factor to explore the connections between these two diseases. The link between diabetes mellitus and cancer has attracted increasing attention in recent years. Emerging evidence supports that oxidatively damaged nucleic acid caused by the imbalance between ROS generation and elimination is a bridge connecting diabetes and cancer.8-oxodG and 8-oxoGuo assume important roles as biomarkers in assessing the relationship between oxidatively damaged nucleic acid and cancer. The consequences of diabetes are extensive and may lead to the occurrence of cancer by influencing a combination of factors. At present, there is no direct evidence that diabetes causes cancer by affecting a single factor. Furthermore, the difficulty in controlling variables and differences in detection methods lead to poor reliability and repeatability of results and there are no clear cut-off values of biomarkers to indicate cancer risk. A better understanding of connections as well as mechanisms between diabetes and cancer is still needed. Both diabetes and cancer are intractable diseases currently. To further explore the specific mechanism of oxidatively damaged nucleic acid in the connection between diabetes and cancer is urgent. In the future, it is necessary to further take oxidatively damaged nucleic acid as an entry point to provide new ideas for the diagnosis and treatment of diabetes and cancer. Experimental drugs targeting the repair process of oxidatively generated damage require an extensive preclinical evaluation and ultimately provide new treatment strategies for these.

15.
Cell Death Dis ; 13(8): 693, 2022 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-35941120

RESUMO

Renal fibrosis is a common consequence of various progressive nephropathies, including obstructive nephropathy, and ultimately leads to kidney failure. Infiltration of inflammatory cells is a prominent feature of renal injury after draining blockages from the kidney, and correlates closely with the development of renal fibrosis. However, the underlying molecular mechanism behind the promotion of renal fibrosis by inflammatory cells remains unclear. Herein, we showed that unilateral ureteral obstruction (UUO) induced Gasdermin D (GSDMD) activation in neutrophils, abundant neutrophil extracellular traps (NETs) formation and macrophage-to-myofibroblast transition (MMT) characterized by α-smooth muscle actin (α-SMA) expression in macrophages. Gsdmd deletion significantly reduced infiltration of inflammatory cells in the kidneys and inhibited NETs formation, MMT and thereby renal fibrosis. Chimera studies confirmed that Gsdmd deletion in bone marrow-derived cells, instead of renal parenchymal cells, provided protection against renal fibrosis. Further, specific deletion of Gsdmd in neutrophils instead of macrophages protected the kidney from undergoing fibrosis after UUO. Single-cell RNA sequencing identified robust crosstalk between neutrophils and macrophages. In vitro, GSDMD-dependent NETs triggered p65 translocation to the nucleus, which boosted the production of inflammatory cytokines and α-SMA expression in macrophages by activating TGF-ß1/Smad pathway. In addition, we demonstrated that caspase-11, that could cleave GSDMD, was required for NETs formation and renal fibrosis after UUO. Collectively, our findings demonstrate that caspase-11/GSDMD-dependent NETs promote renal fibrosis by facilitating inflammation and MMT, therefore highlighting the role and mechanisms of NETs in renal fibrosis.


Assuntos
Armadilhas Extracelulares , Nefropatias , Obstrução Ureteral , Caspases/metabolismo , Armadilhas Extracelulares/metabolismo , Fibrose , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Rim/patologia , Nefropatias/patologia , Macrófagos/metabolismo , Miofibroblastos/metabolismo , Proteínas de Ligação a Fosfato/metabolismo , Proteínas Citotóxicas Formadoras de Poros , Transdução de Sinais , Obstrução Ureteral/genética
16.
Artigo em Inglês | MEDLINE | ID: mdl-35976273

RESUMO

OBJECTIVES: To investigate risk factors and disease burden in pediatric acute recurrent pancreatitis (ARP) and chronic pancreatitis (CP). METHODS: Data were obtained from INternational Study group of Pediatric Pancreatitis: In search for a cuRE-2 (INSPPIRE-2), the largest multi-center prospective cohort study in pediatric patients with ARP or CP. RESULTS: Of 689 children, 365 had ARP (53%), 324 CP (47%). CP was more commonly associated with female sex, younger age at first acute pancreatitis (AP) attack, Asian race, family history of CP, lower BMI%, genetic and obstructive factors, PRSS1 mutations and pancreas divisum. CFTR mutations, toxic-metabolic factors, medication use, hypertriglyceridemia, Crohn disease were more common in children with ARP. Constant or frequent abdominal pain, emergency room (ER) visits, hospitalizations, medical, endoscopic or surgical therapies were significantly more common in CP, episodic pain in ARP. 33.1% of children with CP had exocrine pancreatic insufficiency (EPI), 8.7% had diabetes mellitus. Compared to boys, girls were more likely to report pain impacting socialization and school, medical therapies, cholecystectomy, but no increased opioid use. There was no difference in race, ethnicity, age at first AP episode, age at CP diagnosis, duration of disease, risk factors, prevalence EPI or diabetes between boys and girls. Multivariate analysis revealed that family history of CP, constant pain, obstructive risk factors were predictors of CP. CONCLUSIONS: Children with family history of CP, constant pain or obstructive risk factors should raise suspicion for CP.

17.
Heliyon ; 8(8): e10214, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-36042743

RESUMO

Background: For patients who treated with tacrolimus after kidney transplant, therapeutic drug monitoring is essential to improve their prognosis. However, previous detection methods have limitations, such as the overestimation and unacceptable bias in the immunoassays. Precision medicine has been challenged. The liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is recognized as the gold standard due to its accuracy and specificity, but lack of throughput and complex process limits its clinical application. Therefore, an accurate, simple and high throughput method for tacrolimus monitoring is needed for clinical practice. Methods: A modified LC-MS/MS method was introduced and validated. Whole blood samples were prepared by a one-step protein precipitation method. Chromatographic separation was achieved using a Phenomenex Kinetex 2.6 µm XB-C18 2.1 × 50 mm column with a total run time of 3.5 min to avoid matrix effect. An electrospray ionization source (ESI) was used in positive ion multiple reaction monitoring (MRM) mode for mass spectrometric detection. In order to protect the mass spectrometer, only part of the sample after LC separation was allowed to enter the mass spectrum, through a two HPLC systems coupled one mass spectrometry design. In this way, the instrument throughput is also improved and realizing the detection of 2 samples within 3.5 min and carried out a shorter analyzing time for each sample of 1.75 min. Additionally, we calculated tacrolimus-intrapatient variant (Tac-IPV) based on this modified method and assessed the prognostic value of Tac-IPV in Chinese kidney transplant patients. Results: The LC-MS/MS was modified by streamlining the procedure and increasing the throughput. The method proved to be accurate and reproducible with all performance parameters suitably meeting the clinical requirements over a calibration ranged from 0.37 to 42.90 ng/mL. Parameters such as linearity, limit of quantification (LoQ) and dilution integrity were validated with a clinical reportable range from 0.37 to 343.20 ng/mL, which was particularly useful for high drug concentrations patients (rare but very serious). Both cross-contamination and matrix effects were negligible. Clinical data of 83 patients showed that Tac-IPV was associated with poor kidney transplant outcome in Chinese (Hazard Ratio (HR) = 3.96, 4.75; 95% Cl: 1.10-14.21, 1.23-18.36; P < 0.05). Conclusions: This modified LC-MS/MS method possessed high throughput and simple sample preparation, allowing it to meet daily clinical needs. At the same time, Tac-IPV based on this modified LC-MS/MS had excellent prognostic value in kidney transplantation. These advantages have great significance for the individualized treatment of Chinese kidney transplant patients and broad application of Tac-IPV.

18.
Materials (Basel) ; 15(16)2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-36013637

RESUMO

Polytetrafluoroethylene (PTFE)/aluminum (Al)-based energetic material is a kind of energetic material with great application potential. In this research, the control of the shock-induced energy release characteristics of PTFE/Al-based energetic material by adding oxides (bismuth trioxide, copper oxide, molybdenum trioxide, and iron trioxide) was studied by experimentation and theoretical analysis. Ballistic impact experiments with impact velocity of 735~1290 m/s showed that the oxides controlled the energy release characteristics by the coupling of impact velocities and oxide characteristics. In these experiments, the overpressure characteristics, including the quasi-static overpressure peak, duration, and impulse, were used to characterize the energy release characteristics. It turned out that when the nominal impact velocity was 735 m/s, the quasi-static overpressure peak of PTFE/Al/MoO3 (0.1190 MPa) was 1.99 times higher than that of PTFE/Al (0.0598 MPa). Based on these experimental results, an analytical model was developed indicating that the apparent activation energy and impact shock pressure dominated the energy release characteristic of PTFE/Al/oxide. This controlling mechanism indicated that oxides enhanced the reaction after shock wave unloading, and the chemical and physical properties of the corresponding thermites also affected the energy release characteristics. These conclusions can guide the design of PTFE-based energetic materials, especially the application of oxides in PTFE-based reactive materials.

19.
Environ Res ; 214(Pt 4): 113980, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35998702

RESUMO

Humus (HS) is an important component of soil organic matter. Humic acid (HA) and fulvic acid (FA) are two of the most important components of HS, as they substantially affect biogeochemical processes and the migration and transformation of pollutants in soil. Long-term nitrogen (N) addition can lead to changes in soil physical and chemical properties, affect the structural characteristics of soil HS (HA and FA), cause changes in the adsorption and migration of pollutants, and ultimately result in the continuous deterioration of the soil ecological environment. However, few studies have examined the effects of N addition on the structural characteristics of soil HS, including the responses of soil HA and FA to N addition. Here, we conducted a long-term positioning experiment with different levels of N addition (CK: 0 kg N ha-1 yr-1, LN: 100 kg N ha-1 yr-1, and HN: 300 kg N ha-1 yr-1) in typical farmland soils of the North China Plain to study the response of soil HA and FA to N addition. N addition altered the physical and chemical properties of soil (e.g., pH, SOC, TN, and enzyme activity), which affected the responses of the chemical structure, quality indexes, and composition distribution of soil HA and FA to N addition. Differences in the response to N addition between HA and FA were observed. The structural characteristics of FA were stronger in response to HN compared with those of soil HA. As the level of N added increased, soil FA degradation increased, the composition distribution changed, the aromatization degree and molecular weight decreased, and the molecular structure became simpler. The properties of soil HA did not significantly respond to N addition. Given increases in the global N input (N addition and N deposition), our results have implications for agricultural fertilization, soil management, and other activities.


Assuntos
Poluentes do Solo , Solo , Benzopiranos , Substâncias Húmicas/análise , Nitrogênio , Solo/química , Poluentes do Solo/química
20.
Clin Exp Med ; 2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36018466

RESUMO

Curative surgery and locoregional therapy are radical therapies for patients with HCC. But more than 80% of HCC patients cannot be fitful for radical therapies because of local progression or distant metastasis at initial diagnosis. Among patients with unresectable locally advanced hepatocellular carcinoma (HCC), some patients can be converted to be technically resectable by conversion treatment and salvage surgery. For unresectable locally advanced hepatocellular, conversion treatment prior to salvage surgery with transcatheter arterial chemoembolization (TACE) and other locoregional therapies improve outcomes. PD-1/PD-L1 inhibitors as immune checkpoint inhibitor (ICI) therapy which show high antineoplastic activity in HCC patients by preclinical and clinical researches can also be a good choice for conversion therapy. PD-1/PD-L1 inhibitor combined with locoregional therapy plus antiangiogenic agents or not is most potential conversion therapy comparing to PD-1 inhibitor monotherapy and PD-1/PD-L1 inhibitor combined with antiangiogenic agents or CTLA-4 inhibitor. As more clinical evidence reported, PD-1/PD-L1 immunotherapy would be widely used in conversion treatment of locally advanced hepatocellular carcinoma.

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