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1.
CNS Neurosci Ther ; 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-32243064

RESUMO

AIMS: Both amnestic mild cognitive impairment (aMCI) and remitted late-onset depression (rLOD) confer a high risk of developing Alzheimer's disease (AD). This study aims to determine whether the Characterizing AD Risk Events (CARE) index model can effectively predict conversion in individuals at high risk for AD development either in an independent aMCI population or in an rLOD population. METHODS: The CARE index model was constructed based on the event-based probabilistic framework fusion of AD biomarkers to differentiate individuals progressing to AD from cognitively stable individuals in the aMCI population (27 stable subjects, 6 progressive subjects) and rLOD population (29 stable subjects, 10 progressive subjects) during the follow-up period. RESULTS: AD diagnoses were predicted in the aMCI population with a balanced accuracy of 80.6%, a sensitivity of 83.3%, and a specificity of 77.8%. They were also predicted in the rLOD population with a balanced accuracy of 74.5%, a sensitivity of 80.0%, and a specificity of 69.0%. In addition, the CARE index scores were observed to be negatively correlated with the composite Z scores for episodic memory (R2  = .17, P < .001) at baseline in the combined high-risk population (N = 72). CONCLUSIONS: The CARE index model can be used for the prediction of conversion to AD in both aMCI and rLOD populations effectively. Additionally, it can be used to monitor the disease severity of patients.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32212616

RESUMO

Exploiting an appropriate strategy to prepare fine crystal quality black phosphorus nanosheets (BPNSs) catalyst is a major challenge for its practical application in catalysis. Herein, we address this challenge by developing a rapid electrochemical expansion strategy for scale preparation of fine crystal quality BPNSs from bulk black phosphorus, which was demonstrated to be an active cocatalyst for photocatalytic nitrogen fixation in the presence of CdS as a photocatalyst. The transient photocurrent and charge density studies show that the BPNSs can efficiently accelerate charge separation of CdS, leading to the enhanced photocatalytic activities of BPNSs/CdS nanocomposites for nitrogen fixation. The 1.5% BPNSs/CdS photocatalyst exhibits the highest photocatalytic activity for nitrogen fixation with a NH3 evolution rate of 61.63 µmol•L-1•h-1. This study not only affords a rapid and simple strategy for scale synthesis of fine crystal quality BPNSs but also provides new insight into the design and development of black phosphorus-based materials as low-cost metal-free cocatalyst for photocatalytic nitrogen fixation.

3.
Pharmaceutics ; 12(3)2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32110979

RESUMO

A new platform for triptolide (TP) delivery was prepared by conjugating TP to a carboxylmethyl chitosan (CMCS). Compared with the natural TP, the TP-conjugate (TP-CMCS) containing TP of ~5 wt% exhibited excellent aqueous solubility (> 5 mg/mL). Results of in vitro experiments showed that TP-CMCS could relieve TP-induced inhibition on RAW264.7 cells and apoptosis, respectively. Compared with the TP group, TP-CMCS could effectively alleviate the toxicity injury of TP and decreased the mortality rate of the mice (p < 0.05). TP-CMCS did not cause much damage to the liver (AST and ALT) and kidney (BUN and CRE) (p < 0.05). After administration, the levels of IL-6, IL-1ß, and TNF-α decreased, and the arthritis detumescence percentages increased significantly, and the bony erosion degree was distinctly decreased in the TP-CMCS groups and TP group. Our results suggested that TP-CMCS was a useful carrier for the treatment of RA, which enhanced aqueous solubility of free TP and reduced drug toxicity in vitro and in vivo.

4.
World J Gastroenterol ; 26(8): 839-849, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32148381

RESUMO

BACKGROUND: The impact of body mass index (BMI) on survival in patients with esophageal squamous cell carcinoma (ESCC) undergoing surgery remains unclear. Therefore, a definition of clinically significant BMI in patients with ESCC is needed. AIM: To explore the impact of preoperative weight loss (PWL)-adjusted BMI on overall survival (OS) in patients undergoing surgery for ESCC. METHODS: This retrospective study consisted of 1545 patients who underwent curative resection for ESCC at West China Hospital of Sichuan University between August 2005 and December 2011. The relationship between PWL-adjusted BMI and OS was examined, and a multivariate analysis was performed and adjusted for age, sex, TNM stage and adjuvant therapy. RESULTS: Trends of poor survival were observed for patients with increasing PWL and decreasing BMI. Patients with BMI ≥ 20.0 kg/m2 and PWL < 8.8% were classified into Group 1 with the longest median OS (45.3 mo). Patients with BMI < 20.0 kg/m2 and PWL < 8.8% were classified into Group 2 with a median OS of 29.5 mo. Patients with BMI ≥ 20.0 kg/m2 and PWL ≥ 8.8% (HR = 1.9, 95%CI: 1.5-2.5), and patients with BMI < 20.0 kg/m2 and PWL ≥ 8.8% (HR = 2.0, 95%CI: 1.6-2.6), were combined into Group 3 with a median OS of 20.1 mo. Patients in the three groups were associated with significantly different OS (P < 0.05). In multivariate analysis, PWL-adjusted BMI, TNM stage and adjuvant therapy were identified as independent prognostic factors. CONCLUSION: PWL-adjusted BMI has an independent prognostic impact on OS in patients with ESCC undergoing surgery. BMI might be an indicator for patients with PWL < 8.8% rather than ≥ 8.8%.

5.
Cancer Invest ; 38(3): 158-168, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32073913

RESUMO

Background: Previous studies have reported mixed results regarding the composition of respiratory microbiota in lung cancer patients. Therefore, relying on previously published studies, we sought to estimate the relative proportion of respiratory microbiota between lung cancer cases and controls.Methods: MEDLINE, Embase, The Cochrane Library, and Web of Science online databases were systematically searched from inception up to October 14, 2019, to retrieve relevant studies. The relative abundance of each predominant taxon of respiratory microbiota in lung cancer patients and controls was pooled using the reported outcome data.Results: A total of 8 studies comprising 530 participants were included in the final analysis. The pooled phylum level analysis revealed that Bacteroidetes and Proteobacteria were the most abundant bacterial phyla among all participants, recording 17.5%, 47.5% in lung cancer patients, 28.2%, 39.27% in patients with benign pulmonary diseases and 40.62%, 32.09% in healthy controls, respectively. In addition, Actinobacteria and Firmicutes phyla were abundant in lung cancer cases compared to other groups (14.8%, 17.62% for lung cancer versus 13.04%, 13.16% for benign pulmonary nodules and 12.43%, 12.45% for healthy controls). At genus level, Prevotella was predominant in all the participants, and its proportion was relatively lower in cancer patients (25.74% for lung cancer versus 35.59% and 36.75% for benign pulmonary nodules and healthy controls, respectively). Comparatively, Streptococcus was more abundant in lung cancer cases (9.65% in lung cancer versus 7.98%, 7.26% in benign pulmonary nodules and healthy controls).Conclusions: The respiratory microbiota composition of respiratory microbiota significantly differs between lung cancer patients and healthy individuals, and may be used as potential biomarker of lung cancer. In addition, larger sample size, standardized procedures, dynamic monitoring, metabolomics, and culturomics are needed to confirm these results.


Assuntos
Bactérias/classificação , Disbiose/diagnóstico , Neoplasias Pulmonares/microbiologia , Bactérias/isolamento & purificação , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Filogenia , RNA Ribossômico 16S/genética , Estudos Retrospectivos , Tamanho da Amostra , Análise de Sequência de DNA
6.
J Hazard Mater ; 391: 122213, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32045806

RESUMO

The soil bioelectrochemical system (SBES) is a promising biotechnology for the remediation of contaminated soils. However, the effective distance of pollutant removal in the SBES was usually limited in a few centimeters near the electrode surface. In this study, we used biochar as the model conductor to construct a conductive network with microbes in the soil matrix to extend the effective distance of pollutant removal in the SBES. Pentachlorophenol (PCP) was used as the representative contaminant to probe long-distance electron transfer facilitated by the networks. The removal of PCP and microbial community analyses at different distances toward the electrode were monitored. The results showed that PCP transformation in the SBES without biochar amendment mainly occurred within 4 cm around the electrode. However, the effective distance of ∼ 16 cm toward the electrode could be achieved for efficient PCP degradation in the SBES amended with highly conductive biochar. Microbial community analysis confirmed the establishment of bacteria-biochar networks, where Desulfitobacterium and Geobacter were enriched and spatially distributed in the biochar-amended SBES. The results demonstrate that long-distance electron transfer can be achieved in the biochar-amended soil matrix, and shed light on the development of bioelectrochemical strategy for efficient organic pollutant degradation in soils.

7.
J Diabetes ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040241

RESUMO

BACKGROUND: Limited data on the efficacy of the additional metformin therapy in patients with type 1 diabetes mellitus (T1DM) under real-life conditions have been available so far. METHODS: Patients aged ≥18 years with a duration of T1DM for at least 1 year were included in this multicenter observational study. Patients with insulin combined with metformin therapy (MET group) were compared with those with insulin therapy only (INS group). RESULTS: A total of 76 patients in the MET group were compared with 655 patients in the INS group. At baseline, patients with dyslipidemia were more prevalent in the MET group (17.6% vs 9.0%; P = .006), and they also had a higher body mass index (BMI) (21.7 ± 3.2 kg/m2 vs 20.4 ± 2.6 kg/m2 ; P < .001) than those in the INS group. But glycosylated hemoglobin (HbA1c) and daily insulin dose were not significantly different between the two groups. After 1-year follow-up, HbA1c decreased in both groups, while the daily insulin dose decreased in the MET group, but did not change in the INS group (-0.02 IU/kg [-0.16, 0.09] vs 0 IU/kg [-0.09, 0.09]; P = .029). The additional metformin therapy led to no change of BMI and weight in the MET group, while the body weight increased from 53.7 ± 8.6 kg to 55.0 ± 7.9 kg in the INS group (P < .001). CONCLUSIONS: Metformin is initiated more in T1DM patients with dyslipidemia or higher BMI in current practice in China. The addition of metformin is effective in maintaining weight and reducing the insulin dosage without improving glycemic control in patients with T1DM. HIGHLIGHTS: This is the first study to explore the current status and efficacy of additional metformin therapy in patients with type 1 diabetes mellitus (T1DM) under real-life conditions in China. Metformin is initiated more in T1DM patients with dyslipidemia or higher body mass index in current practice in China. Additional metformin has no effect on glycemic control, but it helps maintain body weight and reduce insulin dosage in adult patients with T1DM.

8.
BMC Plant Biol ; 20(1): 54, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32013882

RESUMO

BACKGROUND: Lead (Pb) pollution is a widespread environmental problem that is harmful to living organisms. Tartary buckwheat (Fagopyrum tataricum), a member of the family Polygonaceae, exhibits short growth cycles and abundant biomass production, could be an ideal plant for phytoremediation due to its high Pb tolerance. Here, we aimed to explore the molecular basis underlying the responses of this plant to Pb stress. RESULTS: In our study, ultrastructural localization assays revealed that Pb ions primarily accumulate in leaf vacuoles. RNA deep sequencing (RNA-Seq) of tartary buckwheat leaves was performed on two Pb-treated samples, named Pb1 (2000 mg/kg Pb (NO3)2) and Pb2 (10,000 mg/kg Pb (NO3)2), and a control (CK). A total of 88,977 assembled unigenes with 125,203,555 bases were obtained. In total, 2400 up-regulated and 3413 down-regulated differentially expressed genes (DEGs) were identified between CK and Pb1, and 2948 up-regulated DEGs and 3834 down-regulated DEGs were generated between CK and Pb2, respectively. Gene Ontology (GO) and pathway enrichment analyses showed that these DEGs were primarily associated with 'cell wall', 'binding', 'transport', and 'lipid and energy' metabolism. The results of quantitative real-time PCR (qRT-PCR) analyses of 15 randomly selected candidate DEGs and 6 regulated genes were consistent with the results of the transcriptome analysis. Heterologous expression assays in the yeast strain Δycf1 indicated that overexpressing CCCH-type zinc finger protein 14 (ZFP14) enhanced sensitivity to Pb2+, while 5 other genes, namely, metal transporter protein C2 (MTPC2), phytochelatin synthetase-like family protein (PCSL), vacuolar cation/proton exchanger 1a (VCE1a), natural resistance-associated macrophage protein 3 (Nramp3), and phytochelatin synthetase (PCS), enhanced the Pb tolerance of the mutant strain. CONCLUSION: Combining our findings with those of previous studies, we generated a schematic model that shows the metabolic processes of tartary buckwheat under Pb stress. This study provides important data for further genomic analyses of the biological and molecular mechanisms of Pb tolerance and accumulation in tartary buckwheat.

9.
10.
Sci Total Environ ; 711: 134539, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32000307

RESUMO

In the present study, the UVC-assisted electrochemical degradation ofthree novel bisphenol analogues (BPs; including bisphenol F, S, and B, i.e., BPF, BPS and BPB, respectively), along with bisphenol A (BPA), was investigated using boron-doped diamond (BDD) electrode. At first, this study demonstrated a significant influence ofcurrent density on the degradation rates of BPF by the BDD anode. The pseudo-first order rate constants for BPF were calculated as 0.012, 0.028 and 0.029 min-1 at the applied current densities of 10, 20 and 30 mA/cm2, respectively. UVC irradiation significantly enhanced the electrochemical degradation of BPF in the concentration range from 5 to 30 mg/L, with synergistic effects in the range of 32.0%-40.9%. The UVC-BDD electrolysisshowed comparable or even lower electric energy per order (EEO) than single BDD electrolysis. The UVC-assisted degradation of the investigated BPs showed decreased pseudo-first order rate constants in the following order: BPF > BPA > BPB > BPS. Based on the identifiedtransformation products, UVC-assisted electrochemical degradation pathways of the novel BPs were proposed to be mainly hydroxylation and bond-cleavage. UVC irradiation has been proved to promote the formation of hydroxyl radicals by BDD electrode to facilitate the degradation process. For these BPs, nearly 100% mineralization can be achieved by a modified strategy using a short-time UVC-assisted BDD electrolysis (120 min) that is followed by UVC photolysis (360 min). Finally, the eco-toxicity of the BPs solutions towardsVibrio Fischeri was significantly removed after 120 min of the electrochemical degradation period. Based on these results, the UVC-assisted electrochemical treatment using a BDD electrode can be considered a promising technology for the removal of novel BPs and the reduction of their hazardous effects to aqueous environments.

11.
Sci Total Environ ; 713: 136605, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-31951842

RESUMO

Purple anoxygenic phototrophs have been recently attracted substantial attention for their growing potential in wastewater treatment and their diverse metabolic patterns can be regulated for process control and optimization. In this study, the photoheterotrophic metabolism of Rhodopseudomonas palustris (R. palustris) was modified by photosynthetic electron uptake using a poised electrode which was explored to enhance removal of veterinary antibiotic from aqueous medium. The results showed that R. palustris grown as biofilm on electrode surface had excellent photoelectroactive activity and the photosynthetic electron uptake from the photoelectroactive biofilm significantly enhanced antibiotic florfenicol (FLO) degradation. The specific degradation rate of FLO at the set electrode potential of 0 V was 2.59-fold higher than that without applied potential. Enhanced co-metabolic reductive dehalogenation by use of the photosynthetic electrons extracted from co-substrate was mainly responsible for FLO degradation which eliminated the antibacterial activity of FLO. The electrode potential controlled the processes of photosynthetic electron uptake and its resultant FLO degradation. The fastest degradation of FLO was achieved at 0 V because the electrode poised at this potential stroke a proper balance between the enhancing photosynthetic electron uptake by serving as electron acceptor and minimizing competition with FLO for the photosynthetic electron from co-substrate. The activity of photoelectroactive biofilm was not negatively affected by FLO at environmental relevant concentration, suggesting its great potential for removal of antibiotic contaminants in wastewater. R. palustris could serve as a reservoir for floR resistance gene but its abundance can be diminished by choosing appropriate electrode potential.


Assuntos
Antibacterianos/isolamento & purificação , Biofilmes , Elétrons , Rodopseudomonas , Águas Residuárias
12.
Sci Total Environ ; 712: 136513, 2020 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-31931188

RESUMO

The performance of microbial electrochemical system for hexavalent chromium (Cr(VI)) contaminant has been a severe challenge remaining active for further development. In this study, we developed a novel biochar material from industrial paper sludge for microbial fuel cell cathode fabrication to reduce aquatic Cr(VI) to non-toxic Cr(III). With additive melamine as nitrogen source and self-containing small portion of Fe as catalyst, the sludge evolved into electroactive biochar (BC-M) rendering a unique N-doped carbon nanotubes/activated carbon (N-CNT/AC) frame after pyrolyzed at 900 °C for 2 h. Electrochemical analysis revealed enhanced electron transference capacity of this composite material, such effectiveness was attributed to the increased surface area and superior electroconductivity of N-doped CNTs. For performance of Cr(VI) reduction, a 55.1% reduction efficiency was achieved in an microbial fuel cell equipped with BC-M cathode while it reduced to about 41.8% when the cathode was replaced by electrode modified with no-melamine-involved biochar. The strategy of biochar upgrading from industrial paper sludge proposed in this work is expected to not only bring technical solution for low-cost CNT materials preparation for Cr(VI) reduction, but also put forward further research on value-added chemical synthesis from waste in various fields of energy and environment.

13.
Ann Transl Med ; 7(20): 549, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31807531

RESUMO

Background: To introduce a modified pleurodesis as an effective treatment for refractory chylothorax and to develop a novel insight for its mechanism. Methods: Patients who underwent thoracic surgery at West China Hospital or its affiliated hospitals between 2010 and 2015 and who subsequently experienced chylothorax that was not resolved by conventional treatment, received daily pleurodesis involving 100 mL 50% glucose and 20 mL 1% lidocaine. The chest tube was clamped after 7 days of pleurodesis, regardless of drainage amount. If no remarkable pulmonary atelectasis was detected within 2 days, the chest tube was removed. All patients were followed up with for at least 3 months after discharge from our hospital. Results: Among the 34 patients, 10 did not experience an increase in the pleural fluid after the chest tube was clamped. Minor effusion increase occurred in 21 patients, while encapsulated effusion occurred in 3. In 23 patients among the latter 24 patients, pleural fluid was gradually absorbed and disappeared spontaneously. One patient suffered chylothorax recurrence after discharge but successfully recovered after the second round of modified pleurodesis. Several patients suffered from electrolyte imbalance, weakness, and dyspnea; all were cured by plasma infusion and other symptomatic treatments. Conclusions: Being safe and effective for patients with postoperative refractory chylothorax, our modified pleurodesis enhanced the process of chemical pleurodesis and could remove the chest tube right after the extensive adhesion formed instead requiring a wait for drainage decrease. This method can thus shorten the period of hospitalization and reduce fluid loss compared with traditional pleurodesis.

14.
Ann Thorac Surg ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794736

RESUMO

For McKeown esophagectomy, gastric tube is widely used for constructing esophagogastrotomy. Traditionally, the gastric fundus is preserved for gastric tube formation in order to provide adequate length of the gastric conduit for neck anastomosis. However, gastric stump necrosis, anastomotic leakage, and postoperative gastric tube dilatation are the common postoperative complications for McKeown esophagectomy using traditional gastric tube. Here, we reported a novel coniform gastric tube shaping technique to maximally avoid conduit dilatation after the McKeown esophagectomy without nasogastric tube placement, while simultaneously maintain a tension-free and well-perfused anastomosis.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31784887

RESUMO

Galectins are an ancient family of lectins characterized by evolutionarily conserved amino acid sequences and ß-galactoside recognition and binding sites. Galectin-3 (Gal-3) is one of 15 known galectins. This protein has important functions in numerous biological activities, including cardiac fibrosis and heart failure. In recent years, many studies have shown that Gal-3 is closely associated with acute myocardial infarction (AMI) and may be a promising biomarker for the assessment of severity as well as prognosis prediction in AMI patients, but controversy still exists. In this review, we summarize the latest literature on the relationship between Gal-3 and unstable plaques, the secretion kinetics of Gal-3 during the acute phase of AMI, and the value of Gal-3 in the prediction of post-AMI remodeling. Finally, the possible value of Gal-3 as a biological target for AMI therapy is examined.

16.
CNS Neurosci Ther ; 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31828965

RESUMO

AIMS: Neuroinflammation is one of the most important processes in the pathogenesis of Parkinson's disease (PD). Sensory disturbances are common in patients with PD, but the underlying pathophysiological mechanisms remain unknown. This study aimed to characterize the activation of Schwann cells (SCs) and the increase of expression of inflammatory cytokines IL-1ß, IL-6, and TNF-α in the sural nerve of PD, and further explore whether peripheral nerve inflammation is the cause of PD sensory disturbances. METHODS: A total of 14 patients with PD (including 5 with sensory disturbances and 9 without sensory disturbances) and 6 controls were included. The excitation and conduction function of sural nerve was detected by sural nerve electrophysiological examination. With sural nerve biopsy samples, ultrastructural changes of sural nerve were observed by electron microscopy; Schwann cell biomarker glial fibrillary acid protein (GFAP) and inflammatory cytokines including interleukin-1beta (IL-1ß), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-α) were detected by immunohistochemistry, and the outcome of immunostaining slice was semiquantitatively counted; double immunofluorescence was used to identify the locus immunoreactive for inflammatory cytokines. RESULTS: Compared with healthy controls, nerve conduction velocity (NCV) slowed down and sensory nerve action potential (SNAP) amplitude decreased in PD patients, accompanied by axonal degeneration and demyelinating lesions, and expression of GFAP and inflammatory cytokines was increased. Inflammatory cytokines were significantly colocalized with GFAP and slightly colocalized with NF. These indicators did not differ significantly between PD patients with and without sensory disturbances. CONCLUSION: Our study results suggest that peripheral sensory nerve injury exists in PD patients, accompanied by Schwann cell activation and inflammation, thus demonstrate peripheral nerve inflammation participates in the pathophysiological process of PD but it is not necessarily related to the patient's sensory disturbance.

17.
Drug Des Devel Ther ; 13: 3091-3103, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695327

RESUMO

Purpose: Polyphyllin VI, a main active saponin isolated from traditional medicinal plant Paris polyphylla, has exhibited antitumor activities in several cancer cell lines. In the present study, we investigated the antitumor effect of Polyphyllin VI against human osteosarcoma cells (U2OS) and the underlying molecular mechanisms. Methods: The U2OS cell lines were used to determine the antiproliferative effect of Polyphyllin VI by CCK8 assay. Cell cycle was analyzed by flow cytometry. The Polyphyllin VI-induced apoptosis was determined by Annexin V-APC/7-AAD apoptosis detection kit and JC-1 staining. Meanwhile, the autophagy was determined by acridine orange staining. The apoptosis and autophagy-related proteins were monitored by Western blot assay. Subsequently, intracellular hydrogen peroxide (H2O2) and the activation of ROS/JNK pathway were detected. Results: Polyphyllin VI could potently inhibit cell proliferation by causing G2/M phase arrest. Polyphyllin VI induced mitochondria-mediated apoptosis with the upregulation of proapoptotic proteins Bax and poly ADP-ribose polymerase, and downregulation of antiapoptotic protein Bcl-2 in U2OS cells. Concomitantly, Polyphyllin VI provoked autophagy with the upregulation of critical Atg proteins and accumulation of LC3B-II. Intracellular H2O2 production was triggered upon exposure to Polyphyllin VI, which could be blocked by ROS scavenger. Polyphyllin VI dramatically promoted JNK phosphorylation, whereas it decreased the levels of phospho-p38 and ERK. Conclusion: Our results reveal that Polyphyllin VI may effectively induce apoptosis and autophagy to suppress cell growth via ROS/JNK activation in U2OS cells, suggesting that Polyphyllin VI is a potential drug candidate for the treatment of osteosarcomas.

18.
Chem Commun (Camb) ; 55(92): 13852-13855, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31670346

RESUMO

An exogenous-oxidant- and catalyst-free electrochemical deoxygenative C2 sulfonylation reaction has been achieved. By employing quinoline N-oxides as the starting materials, the electrochemical C-H sulfonylation of electron-deficient quinolines was indirectly achieved at room temperature and a variety of sulfonylated quinoline derivatives were synthesized in modest to high yield with excellent regioselectivity. Notably, this protocol is the first example for synthesizing sulfonylated electron-deficient heteroarenes/arenes through electrochemistry.

19.
Aging (Albany NY) ; 11(21): 9661-9671, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31699957

RESUMO

Abnormal dopaminergic modulation of the cortico-basal ganglia motor loops results in the emergence of levodopa-induced dyskinesia (LID). We focused on alterations in the gray matter (GM) volume and the cortical thickness of the brain, especially in cortico-basal ganglia motor loops, in Parkinson's disease (PD) with diphasic dyskinesia. 48 PD patients with diphasic dyskinesia, 60 PD patients without dyskinesia and 48 healthy controls (HC) were included. Voxel-based morphometry (VBM) was applied to get GM images from MRI brain images. FreeSurfer was used to get cortical thickness. Distinct analyses of covariance (ANCOVA) and linear contrasts were performed for early- and late-onset PD groups. The severity of diphasic dyskinesia was evaluated by the Unified Dyskinesia Rating Scale (UDysRS). Finally, the correlations between mean volumes of clusters showing differences and the UDysRS scores were performed by Pearson's correlation. The GM volumes of precentral gyri were increased in PD patients with diphasic dyskinesia when compared with those without dyskinesia, which were positively correlated with UDysRS scores in PD patients with diphasic dyskinesia. However, there was no significant difference in cortical thickness among groups. The increased precentral gyri GM volumes might be associated with the pathogenesis and the severity of diphasic dyskinesia.

20.
Ann Transl Med ; 7(18): 444, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31700880

RESUMO

Background: Healthcare-associated infections (HAIs) are still a major health threats worldwide. Traditional surveillance methods involving manual surveillance by infection control practitioners (ICPs) for data collection processes are laborious, inefficient, and generate data of variable quality. In this study, we sought to evaluate the impact of surveillance and interaction platform system (SIPS) for HAIs surveillance compared to manual survey in tertiary general hospitals. Methods: A large multi-center study including 21 tertiary general hospitals and 63 wards were performed to evaluate the impact of electronic SIPS for HAIs. Results: We collected 4,098 consecutive patients and found that the hospitals installed with SIPS significantly increased work efficiency of ICPs achieving satisfactory diagnostic performance of HAIs with 0.73 for sensitivity, 0.81 for specificity and 0.81 area under the curve (AUC). However, there were significant heterogeneity own to regions, time of SIPS installation, departments and sample size. Conclusions: SIPS significantly improved ICPs efficiency and HAIs monitoring effectiveness, but there were shortcomings such as untimely maintenance and high cost.

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