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1.
Front Psychol ; 12: 721783, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34764910

RESUMO

The present event-related potential (ERP) study explored whether masked emotion-laden words could facilitate the processing of both emotion-label words and emotion-laden words in a valence judgment task. The results revealed that emotion-laden words as primes failed to influence target emotion-label word processing, whereas emotion-laden words facilitated target emotion-laden words in the congruent condition. Specifically, decreased late positivity complex (LPC) was elicited by emotion-laden words primed by emotion-laden words of the same valence than those primed by emotion-laden words of different valence. Nevertheless, no difference was observed for emotion-label words as targets. These findings supported the mediated account that claimed emotion-laden words engendered emotion via the mediation of emotion-label words and hypothesized that emotion-laden words could not prime emotion-label words in the masked priming paradigm. Moreover, this study provided additional evidence showing the distinction between emotion-laden words and emotion-label words.

2.
Injury ; 2021 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-34689988

RESUMO

OBJECTIVE: To explore the clinical characteristics and the short-term efficacy of posterior operation for traumatic lumbar spondylolisthesis. METHODS: All 28 patients (between January 2013 and June 2018) were treated with lumbar pedicle screw fixation combined with posterior intervertebral fusion. The clinical data and imaging materials of these patients were retrospectively analyzed. RESULTS: The mean follow-up period was 24.3 months (12-36 months). The average VAS score and JOA score were significantly improved after surgery, and the difference was statistically significant (P<0.05).The last follow-up X-ray showed that 16 cases were degree 0 and 12 cases were degree I according to Meyerding grading, which were statistically improved compared with preoperative. Postoperative CT indicated lumbar internal fixation well, and the lumbar fusion rate was 100%. The Frankel grading of neurological function was significantly improved compared with preoperative. CONCLUSION: Acute traumatic lumbar spondylolisthesis is caused by severe trauma and mostly occurred at L4/L5 and L5/S1 level. Early posterior reduction, decompression and intervertebral fusion can achieve satisfactory clinical and radiological outcome.

3.
PeerJ ; 9: e12027, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34513337

RESUMO

The classification of electroencephalography (EEG) induced by the same joint is one of the major challenges for brain-computer interface (BCI) systems. In this paper, we propose a new framework, which includes two parts, feature extraction and classification. Based on local mean decomposition (LMD), cloud model, and common spatial pattern (CSP), a feature extraction method called LMD-CSP is proposed to extract distinguishable features. In order to improve the classification results multi-objective grey wolf optimization twin support vector machine (MOGWO-TWSVM) is applied to discriminate the extracted features. We evaluated the performance of the proposed framework on our laboratory data sets with three motor imagery (MI) tasks of the same joint (shoulder abduction, extension, and flexion), and the average classification accuracy was 91.27%. Further comparison with several widely used methods showed that the proposed method had better performance in feature extraction and pattern classification. Overall, this study can be used for developing high-performance BCI systems, enabling individuals to control external devices intuitively and naturally.

4.
Nanoscale ; 13(31): 13410-13420, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34477746

RESUMO

Photoacoustic imaging (PAI)-guided photothermal therapy (PTT) has drawn considerable attention due to the deeper tissue penetration and higher maximum permissible exposure. However, current phototheranostic agents are greatly restricted by weak absorption in the second near-infrared (NIR-II, 1000-1700 nm) window, long-term toxicity, and poor photostability. In this report, novel organic NIR-II conjugated polymer nanoparticles (CPNs) based on narrow bandgap donor-acceptor BDT-TBZ polymers were developed for effective cancer PAI and PTT. Characterization data confirmed the high photothermal conversion efficiency, good photostability, excellent PAI performance, and superior biocompatibility of as-obtained CPNs. In addition, in vitro and in vivo tests demonstrated the efficient PTT effect of CPNs in ablating cancer cells and inhibiting tumor growth under 1064 nm laser irradiation. More importantly, the CPNs exhibited rapid clearance capability through the biliary pathway and negligible systematic toxicity. Thus, this work provides a novel organic theranostic nanoplatform for NIR-II PAI-guided PTT, which advances the future clinical translation of biocompatible and metabolizable conjugated nanomaterials in cancer diagnosis and therapy.


Assuntos
Nanopartículas , Neoplasias , Técnicas Fotoacústicas , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Fototerapia , Polímeros , Medicina de Precisão , Nanomedicina Teranóstica
5.
Rev Sci Instrum ; 92(8): 083202, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34470382

RESUMO

Low-noise, high-power 532-nm lasers are of great interest in many scientific research studies, such as gravitational wave detection and ultracold atom experiments. In particular, in the experiments of quantum gas microscopy, a large power of laser is necessary during the imaging process, while low noise is important for preventing the atoms from being heated up. In this work, we report on the generation of such a 532-nm continuous-wave laser by coherently combining two laser beams produced by single-pass second-harmonic generation. The power of the combined laser is up to 17 W. With the help of intensity stabilization, we are able to suppress the relative intensity noise to below -120 dBc/Hz. The generated laser satisfies the experimental requirements for integrating optical superlattices with a quantum gas microscope.

6.
Neuroimage ; 244: 118583, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34562577

RESUMO

To reveal the connectional specialization of the Broca's area (or its homologue), voxel-wise inter-species and individual differences, and inter-hemispheric asymmetry were respectively inspected in humans and macaques at both whole-brain connectivity and single tract levels. It was discovered that the developed connectivity blueprint approach is able to localize connectionally comparable voxels between the two species in Broca's area, whereas the quantitative differences between blueprints of locationally or connectionally corresponding voxels enable us to generate inter-hemispheric, inter-subject, and inter-species connectional variabilities, respectively. More importantly, the inter-species and inter-subject variabilities exhibited positive correlation in both two primates, and relatively higher variabilities were detected in the anatomically defined pars triangularis. By contrast, negative relationship was identified between the inter-species variability and hemispheric asymmetry in human brain. In particular, relatively higher asymmetry was revealed in the anatomically defined pars opercularis. Therefore, our novel findings demonstrated that pars triangularis, as compared to pars opercularis, might be a more active area during primate evolution, in which the brain connectivity and possible functions of pars triangularis show relatively higher degree in species specialization, yet lower in hemispheric specialization. Meanwhile, brain connectivity and possible functions of pars opercularis manifested an opposite pattern. At the tract level, functional roles related to the ventral stream in speech comprehension were relatively conservative and bilaterally organized, while those related to the dorsal stream in speech production show relatively higher species and hemispheric specializations.

7.
Front Endocrinol (Lausanne) ; 12: 721213, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34394010

RESUMO

Background: Type 2 diabetes mellitus (T2DM) patients have a lower risk of abdominal aortic aneurysm (AAA) and its comorbidities, which might be associated with the usage of metformin. The objective of the study was to evaluate the role of metformin in the process of AAA development. Method: PubMed, Embase and Cochrane Library were searched up to May 15th, 2021. We implemented several methods including the risk of bias graph, GRADE system and funnel plot to assess the quality and possible bias of this study. Subgroup analysis and sensitivity analysis were applied to address quality differences and validate the robustness of the final results. Result: Ten articles were enrolled after screening 151 articles searched from databases. The pooled results showed that, compared with T2DM patients without metformin, metformin prescription was associated with a slower annual growth rate of the aneurysm (mean difference (MD) -0.67 cm [95% confidence interval (CI) -1.20 ~ -0.15 cm]). Besides, metformin exposure was associated with a lower frequency of AAA events (odds ratio (OR) 0.61 [95% CI 0.41-0.92]). Conclusion: Metformin alleviated both annual expansion rate and aneurysm rupture frequency in AAA patients with T2DM. Systematic Review Registration: PROSPERO, identifier https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=217859 (CRD42020217859).

8.
Small ; 17(43): e2101332, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34405525

RESUMO

Inorganic nanomedicine has attracted increasing attentions in biomedical sciences due to their excellent biocompatibility and tunable, versatile functionality. However, the relatively poor accumulation and retention of these nanomedicines in targeted tissues have often hindered their clinical translation. Herein, highly efficient, targeted delivery, and in situ aggregation of ferrocene (Fc)-capped Au nanoparticles (NPs) are reported to cucurbit[7]uril (CB[7])-capped Fe3 O4 NPs (as an artificial target) that are magnetically deposited into the tumor, driven by strong, multipoint CB[7]-Fc host-guest interactions (here defined as "supramolecular tropism" for the first time), leading to high tumor accumulation and retention of these NPs. The in vitro and in vivo studies demonstrate the precisely controlled, specific accumulation, and retention of Au NPs in the tumor cells and tissue via supramolecular tropism and in situ aggregation, which afford locally enhanced CT imaging of cancer and enable tumor-specific photothermal therapy attributed to the plasmonic coupling effects between adjacent Au NPs within the supramolecular aggregations. This work provides a novel concept of supramolecular tropism, which may drive targeted delivery and enable specific accumulation, retention, and activation of nanomedicine for improved bioimaging and therapy of cancer.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Neoplasias , Ouro , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Terapia Fototérmica , Tropismo
9.
Asian Nurs Res (Korean Soc Nurs Sci) ; 15(3): 215-221, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34216818

RESUMO

PURPOSE: The aim of this study was to examine the behavioral responses of pregnant women during the early stage of Coronavirus Disease 2019 (COVID-19) outbreak. METHODS: We recruited 1,099 women to complete an online questionnaire survey from February 10 to February 25, 2020. The subjects were divided into two groups (the pregnant women group and the control group). RESULTS: Concerns about infection: most of the participants watched the COVID-19 news at least once a day. Protective behaviors: the utilization rate of pregnant women (often using various measures) was higher than that of nonpregnant women. Exercise: 30.6% of the pregnant women continued to exercise at home, whereas in the control group, this percentage was 8.4%. Spouse relationship: 38.8% of the subjects' relationship improved, whereas only 2.3% thought the relationship was getting worse. CONCLUSION: Pregnant women had some unique behavioral responses different from that of nonpregnant women. It is important to understand the behavioral responses of pregnant women in this network era.


Assuntos
Ansiedade/epidemiologia , Ansiedade/psicologia , COVID-19/psicologia , Depressão/psicologia , Complicações Infecciosas na Gravidez/psicologia , Gestantes/psicologia , Adulto , COVID-19/epidemiologia , China , Estudos Transversais , Depressão/epidemiologia , Feminino , Humanos , Gravidez , Complicações Infecciosas na Gravidez/prevenção & controle
10.
Acta Biomater ; 131: 483-492, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34265471

RESUMO

Photodynamic therapy (PDT), where a photosensitizer (under light irradiation) converts molecular oxygen to singlet oxygen to elicit programmed cell death, is a promising cancer treatment modality with a high temporal and spatial resolution. However, only limited cancer treatment efficacy has been achieved in clinical PDT due to the hypoxic conditions of solid tumor microenvironment that limits the generation of singlet oxygen, and PDT process often leads to even more hypoxic microenvironment due to the consumption of oxygens during therapy. Herein, we designed novel supramolecular micelles to co-deliver photosensitizer and hypoxia-responsive prodrug to improve the overall therapeutic efficacy. The supramolecular micelles (CPC) were derived from a polyethylene glycol (PEG) system dually tagged with hydrophilic cucurbit[7]uril (CB[7]) and hydrophobic Chlorin e6 (Ce6), respectively on each end, for synergistic antitumor therapy via PDT of Ce6 and chemotherapy of a hypoxia-responsive prodrug, banoxantrone (AQ4N), loaded into the cavity of CB[7]. In addition, CPC was further modularly functionalized by folate (FA) via strong host-guest interaction between folate-amantadine (FA-ADA) and CB[7] to produce a novel nanoplatform, AQ4N@CPC-FA, for targeted delivery. AQ4N@CPC-FA exhibited enhanced cellular uptake, negligible cytotoxicity and good biocompatibility, and improved intracellular reactive oxygen species (ROS) generation efficiency. More importantly, in vivo evaluation of AQ4N@CPC-FA revealed a synergistic antitumor efficacy between PDT of Ce6 and hypoxia-activated chemotherapy of AQ4N (that can be converted to chemotherapeutic AQ4 for tumor chemotherapy in response to the strengthened hypoxic tumor microenvironment during PDT treatment). This study not only provides a new nanoplatform for synergistic photodynamic-chemotherapeutic treatment, but also offers important new insights to design and development of multifunctional supramolecular drug delivery system. STATEMENT OF SIGNIFICANCE: Photodynamic therapy (PDT) has exhibited a variety of advantages for cancer phototherapy as compared to traditional chemotherapy. However, the unsatisfactory therapeutic efficacy by PDT alone as a result of the enhanced tumor hypoxia during PDT has limited its clinical application. Herein, we designed multifunctional supramolecular micelles to co-deliver photosensitizer and hypoxia-responsive prodrug to improve the overall therapeutic efficacy. The supramolecular micelles are biocompatible and possess strong red absorption, controlled drug release profile, and ultimately enhanced therapeutic outcome via PDT-chemotherapy. This study not only provides a new nanoplatform for synergistic photodynamic-chemotherapeutic treatment of cancer, but also offers important new insights to design and development of multifunctional supramolecular drug delivery tool for multi-modality cancer therapy.


Assuntos
Antineoplásicos , Fotoquimioterapia , Linhagem Celular Tumoral , Humanos , Hipóxia , Micelas , Fármacos Fotossensibilizantes/farmacologia , Medicina de Precisão
11.
Front Psychiatry ; 12: 659470, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34168579

RESUMO

Background: Prevalence estimates of major depressive disorder (MDD) among adults in China have varied widely between studies. In this systematic review and meta-analysis, the overall prevalence of MDD in the Chinese population was estimated from published epidemiological studies and potential moderators that account for variability in estimates were assessed. Methods: A systematic literature search was conducted in PubMed, EMBASE, Web of Science, PsycINFO, China National Knowledge Internet (CNKI), and WanFang databases to identify relevant studies. Data analyses were conducted using the Comprehensive Meta-Analysis Version 2.0. Results: Forty studies comprising 1,024,087 subjects were included. The pooled point, 12-month, and lifetime prevalence rates of MDD in China were 1.1% (95% CI: 0.9-1.4%), 1.6% (95% CI: 1.0-2.5%), and 1.8% (95% CI: 1.5-2.2%), respectively. Subgroup and meta-regression analyses revealed gender, marital status, survey year, being published in English language, use of the Diagnostic and Statistical Manual of Mental Disorders (DSM) diagnostic systems and age as significant moderators of MDD prevalence. Conclusion: The overall prevalence of MDD in the Chinese population appears to be lower than that of most countries, but the rates have been increasing over time and are elevated in particular demographic subgroups. Due to the negative consequences of MDD, effective preventive measures, early identification, and timely treatments are still important and should be offered to those in need.

12.
Biomaterials ; 275: 120822, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34062419

RESUMO

The hypoxic tumor microenvironment (TME) and non-specific distribution of sonosensitizers are two major obstacles that limit practical applications of sonodynamic therapy (SDT) in combating tumors. Here we report a hypoxia-responsive nanovesicle (hMVs) as delivery vehicles of a sonosensitizer to enhance the efficacy of SDT via specific payload release and local oxygenation in the tumor. The nanovesicles are composed of densely packed manganese ferrite nanoparticles (MFNs) embedded in hypoxia-responsive amphiphilic polymer membranes. With δ-aminolevulinic acid (ALA) loaded in the hollow cavities, the hMVs could rapidly dissociate into discrete nanoparticles in the hypoxic TME to release the payload and induce the generation of reactive oxygen species (ROS) under ultrasound (US) radiation. Meanwhile, the released MFNs could catalytically generate O2 to overcome the hypoxic TME and thus enhance the efficacy of SDT. After treatment, the dissociated MFNs could be readily excreted from the body via renal clearance to reduce long term toxicity. In vitro and in vivo experiments displayed effective tumor inhibition via hMVs-mediated SDT, indicating the great potential of this unique nanoplatform in effective SDT by generating sufficient ROS in deep-seated hypoxic tumors that are not readily accessible by conventional photodynamic therapy.


Assuntos
Hipóxia , Nanopartículas , Linhagem Celular Tumoral , Humanos , Espécies Reativas de Oxigênio , Microambiente Tumoral
13.
ACS Sens ; 6(8): 2858-2867, 2021 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-34185511

RESUMO

Titanium carbide (Ti3C2Tx) with a distinctive structure, abundant surface chemical groups, and good electrical conductivity has shown great potential in fabricating superior gas sensors, but several challenges, such as low response kinetics, poor reversibility, and serious baseline drift, still remain. In this work, γ-poly(l-glutamic acid) (γ-PGA) with a blocking effect is exploited to modify Ti3C2Tx, thereby stimulating the positive response behavior of Ti3C2Tx and improving its gas sensing performance. On account of the unique synergetic interaction between Ti3C2Tx and γ-PGA, the response of the flexible Ti3C2Tx/γ-PGA gas sensor to 50 ppm NO2has been improved to a large extent (average 1127.3%), which is 85 times that of Ti3C2Tx (only 13.2%). Moreover, the as-fabricated Ti3C2Tx/γ-PGA sensor not only exhibits a shorter response/recovery time (average 43.4/3 s) compared with the Ti3C2Tx-based sensor (∼18.5/18.3 min) but also shows good reversibility and repeatability (relative standard deviation (RSD) <1%) at room temperature within 50% relative humidity (RH). The improved gas sensing properties of the Ti3C2Tx/γ-PGA sensor can be attributed to the enhancement of effective adsorption and the blocking effect assisted by water molecules. Furthermore, the gas sensing response of the Ti3C2Tx/γ-PGA sensor is studied at different RHs, and humidity compensation of the sensor is carried out using the multiple regression method. This work demonstrates a novel strategy to enhance the gas sensing properties of Ti3C2Tx by γ-PGA modification and provides a new way to realize highly responsive gas detection at room temperature.


Assuntos
Ácido Glutâmico , Titânio , Adsorção , Umidade , Dióxido de Nitrogênio
14.
Small ; 17(43): e2101397, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34159726

RESUMO

In this study, to visually acquire all-round structural and functional information of lung cancer while performing synergistic photothermal therapy (PTT) and tumor-targeting immunotherapy, a theranostic nanoplatform that introduced upconversion nanoparticles (UCNPs) and IR-1048 dye into the lipid-aptamer nanostructrure (UCILA) is constructed. Interestingly, the IR-1048 dye grafted into the lipid bilayer can serve as the theranostic agent for photoacoustic imaging, optical coherence tomography angiography, photothermal imaging, and PTT in the second near infrared (NIR-II) window. In addition, loaded in the inner part of UCILA, UCNPs possess the superior luminescence property and high X-ray attenuation coefficient, which can act as contrast agents for computed tomography (CT) and thermo-sensitive up-conversion luminescence (UCL) imaging, enabling real-time tracking of metabolic activity of tumor and temperature-feedback PTT. Furthermore, under the complementary guidance of penta-modal imaging and an accurate monitoring of in situ temperature change during PTT, UCILA exhibits its excellent capability for ablating the lung tumor with minimal side effects. Meanwhile, synergistic CAR-NK immunotherapy is carried out specifically to eradicate any possible residual tumor cells after PTT. Therefore, the UCILA nanoplatform is demonstrated as a multifunctional theranostic agent for both penta-modal imaging and temperature-feedback PTT while conducting targeting immunotherapy of lung cancer.


Assuntos
Hipertermia Induzida , Neoplasias Pulmonares , Nanopartículas , Rubiaceae , Linhagem Celular Tumoral , Retroalimentação , Humanos , Imunoterapia , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/terapia , Fototerapia , Terapia Fototérmica , Temperatura , Nanomedicina Teranóstica
15.
Front Endocrinol (Lausanne) ; 12: 674954, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34025585

RESUMO

To examine the effect and mechanism of thyroid hormone on gonadal sex differentiation, Takifugu rubripes larvae were treated with goitrogen (methimazole, MET, 1000 g/g), and thyroxine (T4, 2nM) from 25 to 80 days after hatching (dah). Gonadal histology and sex ratios of fish were then determined at 80 dah. MET treatment induced masculinization, but T4 treatment did not induce feminization in T. rubripes larvae. Transcriptomic analysis of gonads at 80 dah was then conducted. Among the large number of differentially expressed genes between the groups, the expression of foxl2, cyp19a1a, and dmrt1 was altered. The expression of foxl2, cyp19a1a, dmrt1 and gsdf at 25, 40, 55 days after treatment (dat) was further analyzed by qPCR. MET treatment suppressed the expression of foxl2 and cyp19a1a, and induced the expression of dmrt1 in genetic females (p < 0.05). Additionally, T4 treatment induced an increase in the expression of cyp19a1a in genetic XY gonads only at 25 dat. However, the increase in cyp19a1a expression did not continue to 40 and 55 dat. This may explain why feminization of larvae was not found in the T4-treated group. Thus, the present study provides the first evidence that MET treatment causes masculinization in teleost fish. The effects of MET-induced masculinization in T. rubripes may act primarily via suppression of the expression of foxl2 and cyp19a1a, and stimulation of the expression of dmrt1. Moreover, the effects of higher concentrations of T4 or different concentrations of T3, on sex differentiation require further testing.

16.
Front Cardiovasc Med ; 8: 670222, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34046440

RESUMO

Vascular calcification (VC) is an urgent worldwide health issue with no available medical treatment. It is an active cell-driven process by osteogenic differentiation of vascular cells with complex mechanisms. The AMP-activated protein kinase (AMPK) serves as the master sensor of cellular energy status. Accumulating evidence reveals the vital role of AMPK in VC progression. AMPK is involved in VC in various ways, including inhibiting runt-related transcription factor 2 signaling pathways, triggering autophagy, attenuating endoplasmic reticulum stress and dynamic-related protein 1-mediated mitochondrial fission, and activating endothelial nitric oxide synthase. AMPK activators, like metformin, are associated with reduced calcification deposits in certain groups of patients, indicating that AMPK is a potential therapeutic target for VC.

17.
Opt Express ; 29(9): 13876-13886, 2021 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-33985115

RESUMO

Optical superlattice has a wide range of applications in the study of ultracold atom physics. Especially, it can be used to trap and manipulate thousands of atom pairs in parallel which constitutes a promising system for quantum simulation and quantum computation. In the present work, we report on a high-power optical superlattice formed by a 532-nm and 1064-nm dual-wavelength interferometer with a short lattice spacing of 630 nm. The short-term fluctuation (in 10 seconds) of the relative phase between the short lattice and the long lattice is measured to be 0.003π, which satisfies the needs for performing two-qubit gates among neighboring lattice sites. We further implement this superlattice in a 87Rb experiment with a quantum gas microscope of single-site resolution, where the high-power 532-nm laser is necessary for pinning atoms in the short lattice during imaging, providing a unique platform for engineering quantum states.

18.
Biochem Biophys Res Commun ; 555: 182-189, 2021 05 28.
Artigo em Inglês | MEDLINE | ID: mdl-33823364

RESUMO

Autophagy and apoptosis, as major modes of cell death, play critical roles in cellular homeostasis. Our previous study demonstrated that the cross-talk between autophagy and apoptosis regulated cadmium-induced testicular injury and self-recovery, influencing male fertility. However, the underlying mechanism remains blurry. Herein, our subfertility rat model indicated that cadmium-induced autophagy and apoptosis were ameliorated by the activation of SIRT3 and blunted by the inhibition of SIRT3 in rat testis. Further, generating SIRT3 overexpression and knockdown models in TM3 mouse Leydig cells, we found that melatonin (SIRT3 activator) and overexpression of SIRT3 rescued cadmium-induced autophagy and apoptosis in TM3 cells. Knockdown of SIRT3 induced autophagy and apoptosis, which failed to be reversed by melatonin in TM3 cells. Taken together, SIRT3 functions as a pivotal protective factor in testicular Leydig cells injury, and melatonin regulates the cross-talk between autophagy and apoptosis by SIRT3, ameliorating cadmium-induced testicular injury.


Assuntos
Apoptose/fisiologia , Autofagia/fisiologia , Células Intersticiais do Testículo/metabolismo , Melatonina/metabolismo , Sirtuína 3/metabolismo , Adenina/análogos & derivados , Adenina/farmacologia , Animais , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Cádmio/toxicidade , Células Cultivadas , Técnicas de Silenciamento de Genes , Células Intersticiais do Testículo/efeitos dos fármacos , Masculino , Melatonina/farmacologia , Camundongos , Ratos Sprague-Dawley , Sirtuína 3/genética , Sirtuínas/metabolismo , Testículo/citologia , Testículo/efeitos dos fármacos , Testículo/metabolismo
19.
Biomed J ; 44(2): 209-216, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33867286

RESUMO

BACKGROUND: Persistent patent foramen ovale (PFO) and patent ductus arteriosus (PDA) increase the adult risk of cryptogenic embolic stroke and chronic pulmonary hypertension. To understand the characteristics of PFO and PDA in newborns, we investigated the spontaneous closure rate and derived the determinants for residual defects. METHODS: We utilized the database of congenital heart disease (CHD) in Xiamen ChangGung Memorial Hospital from 2015 to 2017 and allocated 2523 eligible newborns into four groups according to PDA, PFO, both or neither at birth. A total of 574, 1229, 202 and 518 newborns were assigned into the group of PFO and PDA, PFO alone, PDA alone and non-PFO/non-PDA, respectively. Regular echocardiographic follow-ups at baseline, 6, 12 and 24 months after birth were performed for evaluating the spontaneous closure rate in the subjects. Regression analysis was carried out to study the risk factors of residual congenital defects. RESULTS: Newborns with PFO alone had the youngest birth age and lowest birth weight among the four groups. About one in four PDA-alone newborns had concomitant small ASD, i.e., <5 mm in diameter. Echocardiographic study showed that 71.3% and 30.8% of CHD newborns had PFO and PDA, respectively, compared to less than 10% of them having ASD or VSD. However, more than 95% of newborns with PFO or PDA closed spontaneously at 6 months, in contrast to about 30% of newborns with ASD or VSD had persistent existence of the intracardiac defects. Complex CHD significantly linked to persistent PFO or PDA at 6 and 12 months, with an adjusted hazard ratio of 9.03 (95% CI 1.97-41.46) and 12.11 (95% CI 2.11-69.72), respectively. CONCLUSIONS: Chinese newborns with PFO or PDA expressed differences in characteristics and concomitant congenital defects. Additionally, persistent PFO or PDA is strongly associated with complex CHD and requires long-term regular monitoring for future associated complications.


Assuntos
Permeabilidade do Canal Arterial , Forame Oval Patente , China , Permeabilidade do Canal Arterial/diagnóstico por imagem , Ecocardiografia , Feminino , Forame Oval Patente/complicações , Forame Oval Patente/diagnóstico por imagem , Humanos , Recém-Nascido , Masculino , Fatores de Risco , Resultado do Tratamento
20.
Endocrinology ; 162(10)2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33831176

RESUMO

Elucidating the global molecular changes that occur during aromatase inhibitor (AI)- or 17α-methyltestosterone (MT)-induced masculinization and estradiol-17ß (E2)-induced feminization is critical to understanding the roles that endocrine and genetic factors play in regulating the process of sex differentiation in fish. Here, fugu larvae were treated with AI (letrozole), MT, or E2 from 25 to 80 days after hatching (dah), and gonadal transcriptomic analysis at 80 dah was performed. The expression of dmrt1, gsdf, foxl2, and other key genes (star, hsd3b1, cyp11c1, cyp19a1a, etc.) involved in the steroid hormone biosynthesis pathway were found be altered. The expression of dmrt1, gsdf, cyp19a1a, and foxl2 was further verified by quantitative polymerase chain reaction. In the control group, the expression of dmrt1 and gsdf was significantly higher in XY larvae than in XX larvae, while the expression of foxl2 and cyp19a1a was significantly higher in XX larvae than in XY larvae (P < .05). AI treatment suppressed the expression of foxl2 and cyp19a1a, and induced the expression of dmrt1 and gsdf in XX larvae. MT treatment suppressed the expression of foxl2, cyp19a1a, dmrt1, and gsdf in XX larvae. E2 treatment suppressed the expression of dmrt1 and gsdf, but did not restore the expression of foxl2 and cyp19a1a in XY larvae. The shared response following AI, MT, and E2 treatment suggested that these genes are essential for sex differentiation. This finding offers some insight into AI or MT-induced masculinization, and E2-induced femininization in fugu.

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