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1.
Medicine (Baltimore) ; 100(9): e24166, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33655910

RESUMO

BACKGROUND: The purpose of this meta-analysis was to evaluate the clinical efficacy and safety of HR and PFNA in the treatment of intertrochanteric fractures in the elderly. METHODS: We carried out this review according to the principle of preferred reporting items for systematic reviews and meta-analyses (PRISMA) guideline. The clinical randomized controlled trials (RCTs), prospective cohort studies, retrospective cohort studies (RCSs), and case-control studies involving HR and PFNA in the treatment of intertrochanteric fractures in the elderly from 2000 to 2020 were compared by searching Web of Science, Pubmed, the Cochrane Library, and Embase. The quality of the included cohort study (CS) lines was evaluated using the Newcastle-Ottawa Scale (NOS). The quality of the included RCT lines was evaluated using Jadad. Forest plots were drawn by RevMan5.4 software based on the results and the data were analyzed. RESULTS: After screening, a total of 9 articles were included, of which one was a clinical RCT and eight were RCSs with 1374 patients. The operative time of the PFNA group was shorter [WMD = 15.20; 95% CI (13.17, 17.23), P < .05] and the intraoperative blood loss was less [WMD = 178.81; 95% CI (97.24, 260.38), P < .05] than the HR group, while the first weight-bearing time of the HR group was shorter [WMD = -7.70; 95% CI (-10.54, -4.86), P  < .05] than the PFNA group. There was no significant difference in the length of hospital stay, HHS, postoperative orthopedic complications, and postoperative medical complications between the 2 groups. CONCLUSION: With the development of HR technology and minimally invasive technology, the trauma caused by surgery is decreasing. Under the premise of improving perioperative management, such as optimizing the preoperative preparation and postoperative management, shortening the operative time, reducing intraoperative blood loss, and actively managing co-existing diseases, HR has more advantages than PFNA in the treatment of senile intertrochanteric fractures.


Assuntos
Artroplastia de Quadril/estatística & dados numéricos , Pinos Ortopédicos , Fixação Interna de Fraturas/estatística & dados numéricos , Fraturas do Quadril/cirurgia , Idoso , Perda Sanguínea Cirúrgica/estatística & dados numéricos , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Estudos Prospectivos , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Retrospectivos , Resultado do Tratamento
2.
Zhongguo Zhong Yao Za Zhi ; 46(5): 1128-1133, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33787106

RESUMO

On the basis of the previous work of the research group, the orthogonal design method was further used to optimize the processing technology for reducing toxicity of fried Tripterygium wilfordii in Lysimachia christinae Decoction. A total of 9 processed products of T.wilfordii in L.christinae decoction were prepared by four factors and three levels orthogonal design table. The contents of triptolide in T.wilfordii were determined by high performance liquid chromatography(HPLC) before and after processing: 4.27, 3.92, 3.57, 2.75, 2.42, 2.66, 3.51, 1.87, 1.75, 2.03 µg·g~(-1). On this basis, the above processed products were orally given to mice for 28 days. 12 hours after the last administration, food fasting except water was provided, and 24 hours later, the eyeballs were taken for blood and liver tissue. Serum biochemical indexes, liver lipid peroxidation and antioxidant related indexes were detected by kit method. Twenty-eight days after oral administration of raw T.wilfordii, the levels of serum alanine aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP) and liver malondialdehyde(MDA) in mice increased by 91%(P<0.01), 46%(P<0.05), 73%(P<0.01) and 99%(P<0.01), while the liver antioxidant indexes such as superoxide dismutase(SOD), glutathione(GSH), glutathione peroxidase(GPX) and glutathione-S transferase(GST) significantly decreased(P<0.01). After administration of the processed products, the above indexes were significantly reversed(P<0.01 or P<0.05). Especially, the processing conditions of A_3B_2C_1D_3 had the best detoxification effect on T.wilfordii, which decreased the high levels of AST, ALT, ALP and MDA by 49%(P<0.01), 32%(P<0.01), 42%(P<0.01), and 17%(P<0.05). Therefore, the best processing conditions for T.wilfordii in L.christinae decoction were A_3B_2C_1D_3, namely "15% mass fraction of L.christinae, 1 h moistening time, 160 ℃ frying temperature, and 9 min frying time".


Assuntos
Primulaceae , Tripterygium , Animais , Antioxidantes , Fígado , Camundongos , Tecnologia
3.
Sci Total Environ ; 767: 145497, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33579558

RESUMO

The air purification potential of plants has been widely studied and recognized. However, their specific capacities in retaining water-soluble (WSPM) and water-insoluble (WIPM) atmospheric particulate matter (PM) are still unclear. In order to recommend tree species with high air phytoremediation ability, the retention characteristics for WSPM and WIPM of five tree species under different haze pollution levels and PM retention durations in Beijing were evaluated after introducing ultrasonic cleaning procedure to the conventional leaf cleaning methods. The daily PM amount retained these species in the six central districts in Beijing (SCBD) was roughly estimated based on the field tree survey data in 171 plots randomly distributed within the Fifth Ring Road. The updated leaf cleaning method improved the evaluation accuracy for WSPM and WIPM by 54% and 31%, respectively. The particles retained by the broadleaf and coniferous species were mainly composed of WSPM (71%) and WIPM (64%), respectively. The diameter distribution of PM varied markedly with species, PM retention duration, and pollution level. However, it always showed a unimodal pattern for WSPM and no uniform patterns for WIPM. The average relative capacities of different species in retaining WSPM of TSP (PM ≤ 100 µm) were more stable with time, and the corresponding rank was Sophora japonica > Salix babylonica > Ginkgo biloba > Pinus tabuliformis > Sabina chinensis. Whereas, as to the WIPM of TSP, their order changed to S. japonica > P. tabuliformis > S. babylonica > G. biloba > S. chinensis. During the study period, the TPM (WIPM+WSPM) of TSP retained by these species per day in the SCBD reached 132.6 t (76.1 t WSPM + 56.5 t WIPM), accounting for a considerable proportion of the daily dust-fall amount. These findings can contribute to selecting greening tree species and managing the urban forest to improve urban air quality.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Pequim , China , Monitoramento Ambiental , Material Particulado/análise , Folhas de Planta/química , Árvores , Água
4.
Cancer Biomark ; 2021 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-33554888

RESUMO

BACKGROUND: Platelets play a key role in tumor progression and metastasis. C-type lectin-like receptor 2 (CLEC-2) is the receptor expressed on platelets and the marker of platelet activation. OBJECTIVE: This study aims to determine whether soluble CLEC-2 levels differ between patients with benign colorectal polyps and those with colorectal cancer (CRC). METHODS: We measured plasma soluble CLEC-2 by enzyme-linked immunosorbent assay in 150 patients with colorectal polyps, 150 CRC patients without metastasis, 150 CRC liver metastasis, and 150 control subjects. RESULTS: The CRC patients had higher soluble CLEC-2 levels than patients with colorectal polyps (p< 0.001). Moreover, CRC patients with liver metastases displayed higher CLEC-2 levels than those in CRC patients without metastases (p< 0.001). In the CRC patients, CLEC-2 levels were correlated with lymph node metastasis and advanced stage. In the patients with polyps, there was a significant difference in CLEC-2 levels among patients with hyperplastic polyp, sessile serrated adenoma, and traditional serrated adenoma (p< 0.001). The ROC curve analysis revealed CLEC-2 had an optimal sensitivity of 77.3% and specificity of 94.6% for the screening of CRC, and sensitivity of 71.0% and specificity of 76.7% for the differential diagnosis of colorectal polyps and CRC. CONCLUSIONS: CRC patients have higher CLEC-2 levels than patients with colorectal polyps and healthy controls. Moreover, there is a significant difference in CLEC-2 levels among polyp subtypes. Further research is warranted.

5.
Bioengineered ; 12(1): 527-539, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33535880

RESUMO

The effects of polyaspartic acid and different controlled-release fertilizers with urea on dry matter accumulation and distribution, nitrogen absorption and accumulation, and the activities of enzymes involved nitrogen metabolism and yield of corn were studied by using xianyu (XY688), a maize nitrogen efficient cultivar, and Jifeng NO.2 (JF2), a maize nitrogen-inefficient cultivar, as experimental materials and through random blocks experimental design in 2019. For XY688, polyaspartic acid chelated nitrogen fertilizer (PASPN) had the highest yield, which was 21.34% higher than N0 treatment. For JF2, it also had the highest yield under PASPN combined urea treatment, which was 23.44% higher than N0 (no nitrogen fertilizer), and JF2 had a 9.7% lower yield under XY688 treatment. For XY688, PASPN treatment had the largest nitrogen uptake in grain, up to 3.14 kg/hm2, and PASPN treatment increased 17.4% compared with N0. For JF2, grain nitrogen uptake was also the highest under PASPN treatment, which was significantly different from other treatments. Nitrogen uptake was 3.16 kg/hm2, which increased 37.4% compared with N0. Compared with JF2, XY688 showed higher nitrogen uptake efficiency, nitrogen utilization efficiency, and partial nitrogen productivity. For XY688, the highest nitrogen absorption efficiency was SU3 (slow-release urea and ordinary urea) treatment (0.36 kg/kg). The partial nitrogen productivity and harvest index of PASPN treatment were the highest and significantly different from other treatments. The partial nitrogen productivity of PASPN treatment was 57.02 kg/kg. These results can provide help for the further researches of the rational utilization and absorption of nitrogen fertilizer.

6.
Preprint | medRxiv | ID: ppmedrxiv-21250506

RESUMO

BackgroundDuring the current Coronavirus Disease 2019 (COVID-19) pandemic, diabetic patients face disproportionately more. Anti-inflammatory effects of hypoglycemic agents have been reported, and their beneficial or harmful effects in patients with diabetes and COVID-19 remain controversial. PurposeThis study was performed to clarify this association. Data SourcesRelevant literature was searched on China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform, Chinese periodical service platform VIP Database, Sinomed (China Biology Medicine, CBM), MedRxiv, PubMed, ScienceDirect, Web of Science, Ovid Databases (LWW), Springer Link, Wiley Online Library, Oxford Academic, Nature Press Group, Cochrane Library and BMJ Evidence-Based Medicine up to November 14, 2020. Study SelectionOnly observational studies of hypoglycemic agents vs. drugs or therapy without hypoglycemic agents in adult diabetic patients with COVID-19 were included. Data ExtractionData of death and poor composite outcomes were extracted. Data SynthesisThe pooled effects were calculated using the fixed-effects or random-effects models based on heterogeneity assessment. LimitationMost studies were retrospective cohort studies with relative weak capability to verify causality. ConclusionHome use of metformin might be beneficial in decreasing mortality in diabetic patients infected with SARS-CoV-2. There is insufficient evidence to conclude that metformin and other hypoglycemic agents are associated with poor composite outcomes. More prospective studies, especially RCTs are needed. Registration-PROSPEROCRD42020221951.

7.
Medicine (Baltimore) ; 100(1): e24134, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33429787

RESUMO

ABSTRACT: Ovarian cancer (OC), a common malignant heterogeneous gynecological tumor, is the primary cause of cancer-related death in women worldwide. Adenylate kinase (AK) 7 belongs to the adenylate kinase (AK) family and is a cytosolic isoform of AK. Recent studies have demonstrated that AK7 is expressed in several human diseases, including cancer. However, there is a scarcity of reports on the relationship between AK7 and OC. Here, we compared the expression of AK7 in normal and cancerous ovarian tissues from The Cancer Genome Atlas database and used the c2 test to assess the correlation between AK7 levels and the clinical symptoms of OC. Finally, the prognostic significance of AK7 in OC was determined using the Kaplan-Meier analyses and Cox regression and performed gene set enrichment analysis to detect any relevant signaling pathways. We found that AK7 levels were substantially downregulated in OC than that in normal ovarian tissues (P < .001). Low AK7 levels were related to the patients' age (P = .0093) in OC. The median overall survival (OS) of patients with low AK7-expressing OC was shorter than patients with high AK7-expressing OC (P = .019). The Cox regression analysis (multivariate) identified low AK7 levels were independently related to the prognosis of OC (HR 1.34; P = .048). Our study demonstrated that the downregulated levels of AK7 could serve as an independent prognostic indicator for the OS in OC. Additionally, gene set enrichment analysis revealed that EMT, apical junction, TGF-b signaling, UV response, and myogenesis were associated in the low AK7 expression phenotype (NOM P < .05).


Assuntos
Adenilato Quinase/análise , Neoplasias Ovarianas/complicações , Prognóstico , Adenilato Quinase/sangue , Adenilato Quinase/genética , Idoso , Biomarcadores Tumorais/análise , Biomarcadores Tumorais/sangue , Regulação para Baixo , Feminino , Humanos , Estimativa de Kaplan-Meier , Pessoa de Meia-Idade , Neoplasias Ovarianas/sangue , Neoplasias Ovarianas/classificação
8.
Huan Jing Ke Xue ; 42(1): 114-126, 2021 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-33372463

RESUMO

The objective of this study was to explore the differences of five methods for evaluating the PM retention capacity of leaves based on the same experimental materials and leaf area measurement method and to summarize the advantages and disadvantages of each method. In this study, four tree species (Pinus tabuliformis, Platycladus orientalis, Ginkgo biloba, and Platanus occidentalis), which are common in Beijing and have greatly different leaf characteristics, were selected as the research objects. The mass subtraction method (MS), the membrane filter method (MF), the aerosol regenerator method(AR), the scanning electron method (SEM), and the elution weighing method coupled with a particle size analysis based on ultrasonic cleaning (ultrasonic-EWPA) were used to evaluate the PM retention capabilities and characteristics of the leaves of the four tree species. The total time needed and the total cost were measured simultaneously during the evaluation process. The results showed that although the values of PM retention efficiency obtained by different methods were quite different, the ranks of the efficiency of four tree species obtained by different methods were the same or partially the same. Additionally, the results obtained by the methods with the same or similar principles were more overlapped (AR and SEM had the most overlapped results). In addition, 89% of the species ranks of the same index obtained by each method were P. orientalis > P. tabuliformis > P. occidentalis > G. biloba, and the remaining 11% were P. occidentalis > G. biloba > P. orientalis > P. tabuliformis. Among the five methods, ultrasonic-EWPA was the one with the largest number of indexes and the highest cost, and MS was the one with the least number of indexes and the lowest cost. The one that needed most time was MF, while the one that needed the least time was AR. ultrasonic-EWPA and SEM are high input and high output methods. That is to say, although they needed more time and cost, they can prove more information; however, MS was opposite, which resulted in less information but lower time and cost needed. So, it is suitable for roughly evaluating the total PM retention capacities of trees; MF had a medium amount of information, low cost, but required too much time, which needs to be weighed and balanced before selecting this method. The AR method had strict requirements for equipment and parameters and should be used with caution. The results of this study can provide a comprehensive and detailed scientific basis for researchers to choose specific methods in the future.


Assuntos
Poluentes Atmosféricos , Material Particulado , Poluentes Atmosféricos/análise , Pequim , Monitoramento Ambiental , Material Particulado/análise , Folhas de Planta/química
9.
Entropy (Basel) ; 22(2)2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33285977

RESUMO

In this paper, bright-dark, multi solitons, and other solutions of a (3 + 1)-dimensional cubic-quintic complex Ginzburg-Landau (CQCGL) dynamical equation are constructed via employing three proposed mathematical techniques. The propagation of ultrashort optical solitons in optical fiber is modeled by this equation. The complex Ginzburg-Landau equation with broken phase symmetry has strict positive space-time entropy for an open set of parameter values. The exact wave results in the forms of dark-bright solitons, breather-type solitons, multi solitons interaction, kink and anti-kink waves, solitary waves, periodic and trigonometric function solutions are achieved. These exact solutions have key applications in engineering and applied physics. The wave solutions that are constructed from existing techniques and novel structures of solitons can be obtained by giving the special values to parameters involved in these methods. The stability of this model is examined by employing the modulation instability analysis which confirms that the model is stable. The movements of some results are depicted graphically, which are constructive to researchers for understanding the complex phenomena of this model.

10.
Preprint | medRxiv | ID: ppmedrxiv-20248860

RESUMO

BackgroundPrevious researches on the association between proton pump inhibitors (PPIs) use and the treatment and prevention of COVID-19 have generated inconsistent findings. Therefore, this Meta-analysis was conducted to clarify the outcome in patients who take PPIs. MethodsWe carried out a systematic search to identify potential studies until November 2020. Heterogeneity was assessed using the I-squared statistic. Odds ratios (ORs) with its 95% confidence intervals (CIs) were calculated by fixed-effects or random-effects models according to the heterogeneity. Sensitivity analyses and tests for publication bias were also performed. ResultsEight articles with more than 268,683 subjects were included. PPI use was not associated with increased or decreased risk of COVID-19 infection (OR:3.16, 95%CI = 0.74-13.43, P=0.12) or mortality risk of COVID-19 patients (OR=1.91, 95% CI=0.86-4.24, P=0.11). While it can add risk of severe disease (OR=1.54, 95% CI=1.20-1.99, P<0.001;) and secondary infection (OR=4.33, 95% CI=2.57-7.29). No publication bias was detected. ConclusionsPPI use is not associated with increased risk infection and may not change the mortality risk of COVID-19, but appeared to be associated with increased risk of progression to severe disease and secondary infection. However, more original studies to further clarify the relationship between PPI and COVID-19 are still urgently needed.

11.
Fitoterapia ; 147: 104774, 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33152461

RESUMO

Nine new diterpenoids, Rubellacrns A - I (1-9), including five isopimaranes (1-4, 9), four pimaranes (5-8), together with five known isopimarane analogues (10-14), were isolated from Callicarpa rubella. The structures of these compounds were unambiguously established by HR-ESIMS and NMR spectroscopic data, the absolute configurations of compounds 5 and 9 were determined by ECD. All the isolated compounds were tested for their anti-inflammatory effects and compounds 2 and 11-14 showed NLRP3-inflammasome inhibitory activity with IC50 values ranging from 7.02 to 14.38 µM.

12.
Inorg Chem ; 59(20): 14920-14931, 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-32951429

RESUMO

Photodynamic therapy (PDT) using two-photon near-infrared light excitation is a very effective way to avoid the use of short-wavelength ultraviolet or visible light which cannot efficiently penetrate into the biological tissues and is harmful to the healthy cells. Herein, a series of cyclometalated Ir(III) complexes with a structurally simple diimine ligand were designed and the synthetic route and preparation procedure were optimized, so that the complexes could be obtained in apparently higher yield, productivity, and efficiency in comparison to the traditional methods. Their ground state and excited singlet and triplet state properties were studied by spectroscopy and quantum chemistry theoretical calculations to investigate the effect of substituent groups on the photophysical properties of the complexes. The Ir(III) complexes, especially Ir1 and Ir3, showed very low dark toxicities and high phototoxicities under both one-photon and two-photon excitation, indicating their great potential as PDT agents. They were also found to be highly sensitive two-photon mitochondria dyes.

13.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 34(9): 1130-1135, 2020 Sep 15.
Artigo em Chinês | MEDLINE | ID: mdl-32929906

RESUMO

Objective: To analyze the effectiveness of percutaneous injection of autologous concentrated bone marrow aspirate (cBMA) combined with platelet-rich plasma (PRP) in the treatment of delayed fracture healing. Methods: A prospective, randomized, controlled, single-blind case study was conducted. Between March 2016 and July 2018, 66 patients who met the inclusion and exclusion criteria for delayed fracture healing but had solid internal fixation of the fracture end were randomly divided into control group (31 cases, treated with percutaneous autogenous bone marrow blood injection) and study group (35 cases, treated with percutaneous autogenous cBMA+PRP injection). General data such as gender, age, body mass index, site of delayed fracture healing, length of bone defect at fracture end, and preoperative radiographic union score for tibia (RUST) showed no significant difference between the two groups ( P>0.05). Before injection, Kirschner wire was used in both groups to stimulate the fracture end and cause minor injury. The fracture healing time, treatment cost, and adverse reactions were recorded and compared between the two groups. Visual analogue scale (VAS) score was used to evaluate pain improvement. The tibial RUST score was extended to the tubular bone healing evaluation. Results: No infection of bone marrow puncture needle eyes occurred in both groups. In the control group, local swelling was obvious in 5 cases and pain was aggravated at 1 day after operation in 11 cases. In the study group, postoperative swelling and pain were not obvious, but 2 cases presented local swelling and pain. All of them relieved after symptomatic treatment. Patients in both groups were followed up, the follow-up time of the control group was 16-36 months (mean, 21.8 months), and the study group lasted 14-33 months (mean, 23.2 months). The amount of bone marrow blood was significantly lower in the study group than in the control group ( t=4.610, P=0.000). The degree of postoperative pain in the study group was less than that in the control group, and the treatment cost was higher than that in the control group. But the differences between the two groups in VAS score at 1 day after operation and treatment cost were not significant ( P>0.05). Fracture healing was achieved in 19 cases (61.3%) in the control group and 30 cases (85.7%) in the study group. The difference in fracture healing rate between the two groups was significant ( χ 2=5.128, P=0.024). Fracture healing time and RUST score at last follow-up were significantly better in the study group than in the control group ( P<0.05). At last follow-up, RUST scores in both groups were significantly improved when compared with those before operation ( P<0.05). Conclusion: Autogenous cBMA combined with PRP percutaneous injection can provide high concentration of BMSCs and growth factors, and can improve the fracture healing rate and shorten the fracture healing time better than autogenous bone marrow blood injection.


Assuntos
Consolidação da Fratura , Plasma Rico em Plaquetas , Medula Óssea , Fixação Interna de Fraturas , Humanos , Estudos Prospectivos , Método Simples-Cego , Resultado do Tratamento
14.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 34(8): 1012-1017, 2020 Aug 15.
Artigo em Chinês | MEDLINE | ID: mdl-32794671

RESUMO

Objective: To investigate the effectiveness of the treatment under the guidance of "diamond concept" for femoral shaft fractures nonunion after intramedullary fixation. Methods: Between January 2014 and December 2016, 21 cases of femoral shaft fractures nonunion after intramedullary fixation were treated with auxiliary plate fixation combined with autogenous iliac graft, and autologous bone marrow concentrate and platelet-rich plasma (PRP) gel under the guidance of the "diamond concept". There were 13 males and 8 females, with an average age of 32.5 years (range, 17-48 years). All fractures were closed femoral shaft fractures. Four patients underwent internal fixation with plate and resulted in nonunion, then they were fixed with intramedullary nails, but did not heal either. The rest 17 patients were fixed with intramedullary nailing. Fracture nonunion classification: 4 cases of hypertrophic nonunion, 17 cases of atrophic nonunion; the length of bone defect was 1-3 mm; the duration from the last treatment to the current treatment was 10-23 months (mean, 14.3 months). The operation time, intraoperative blood loss, the time between operation and full loading, fracture healing time, and complications were recorded. The visual analogue scale (VAS) score and the imaging system of fracture healing of the extremities (RUST) of patients before operation and at last follow-up were recorded to evaluate the fracture healing; the function of the affected limb was evaluated according to the Schatzker-Lambert efficacy score standard at last follow-up. Results: The operation time was 105-160 minutes, with an average of 125.6 minutes; the intraoperative blood loss was 160-580 mL, with an average of 370.5 mL. All incisions healed by first intention, without vascular or nerve injury. All patients were followed up 22-46 months (mean, 26.5 months). All the fractures healed, with a fracture healing time of 3-7 months (mean, 4.8 months). During the follow-up, there was no infection, loosening, implant breakage, re-fracture, and other complications. The VAS score at last follow-up was 0.8±0.3, showing significant difference ( t=7.235, P=0.000) when compared with preoperative score (5.2±3.7); the RUST score was 3.4±0.3, which was significantly higher than the preoperative score (1.5±0.7) ( t=8.336, P=0.000). According to the Schatzker-Lambert effectiveness evaluation standard, the limb function was excellent in 16 cases, good in 4 cases, fair in 1 case, and the excellent and good rate was 95.42%. Conclusion: Nonunion after intramedullary fixation of femoral fracture treated with auxiliary plate combined with autogenous iliac graft, autogenous bone marrow concentration and PRP gel in accordance with the "diamond concept" can not only restore the stability of the fracture ends, but also improves the biological environment of the fracture site, and can improve the rate of fracture healing.


Assuntos
Fraturas do Fêmur , Fixação Intramedular de Fraturas , Adolescente , Adulto , Pinos Ortopédicos , Diamante , Diáfises , Feminino , Consolidação da Fratura , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Adulto Jovem
15.
Bioresour Technol ; 314: 123723, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32599527

RESUMO

Pretreatment is an essential upstream process to deconstruct oil palm empty fruit bunch fiber (OPEFBF) prior to sugars production. This study aimed to investigate the efficiency of OPEFBF pretreatment using palm oil mill effluent (POME) as solvent. The effect of alkali catalyst (5%w/w NaOH and ammonia), temperature (90,120,135 °C) and time (60,120,180 min) on the efficiency of pretreatment (OPEFBF-to-solvent ratio of 1:25) was also investigated. The results indicated that POME-pretreatment (135 °C, 180 min) enhanced glucose yield by only ~56%. Glucose production was increased about 5.8-fold to 495.3 ± 5.9 mg g-1 OPEFBF when NaOH was added in POME-pretreatment (Na-P). The xylose production from OPEFBF was increased about 3.7-fold after ammonia-catalyzed POME-pretreatment. About 12.1 ± 0.2 g L-1 of ethanol was produced from Na-P-hydrolysate at molar conversion of 59.4 ± 1.4%. This research provides new insight into the use of POME as a cost-effective pretreatment solvent of OPEFBF to reduce upstream process cost by cutting down water usage.


Assuntos
Frutas , Açúcares , Carboidratos , Óleo de Palmeira , Óleos Vegetais , Solventes
16.
J Clin Nurs ; 29(15-16): 2863-2871, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32320100

RESUMO

AIMS AND OBJECTIVES: To evaluate the efficacy and safety of a comprehensive protocol for constipation prevention. BACKGROUND: Constipation is a common problem for patients undergoing total hip arthroplasty (THA), yet sparse evidence is available to guide constipation prevention after THA. DESIGN: Randomised controlled superiority clinical trial. METHODS: This randomised controlled study was carried out according to the Consolidated Standards of Reporting Trials (CONSORT). A total of 80 THA patients were randomised to receive only preoperative education about lifestyle or the combination of education with postoperative abdominal massage and polyethylene glycol 4,000 (Forlax®). Efficacy outcomes included rates of postoperative constipation and enema rescue, as well as time to first postoperative defecation and readmission within 30 days. Safety outcomes were number and type of adverse events. RESULTS: Patients who received combination treatment showed a significantly lower rate of postoperative constipation during hospitalisation than patients who received only preoperative education (25% versus 55%), and they showed a significantly lower rate of enema rescue (12.5% versus 40%). Many more patients receiving combination treatment experienced their first defecation within two postoperative days than patients who received only preoperative education (62.5% versus 35.9%). In contrast, the two groups were similar in terms of constipation rate on postoperative days 15 and 30, rate of readmission within 30 days and rate of postoperative adverse events. CONCLUSIONS: These results suggest that our comprehensive protocol can relieve constipation after THA, reduce the need for enema rescue and shorten time to first defecation without sacrificing safety. More work is needed to optimise and develop this protocol further. RELEVANCE TO CLINICAL PRACTICE: Constipation is a distressing problem that frequently occurs after THA. This study confirmed that a comprehensive protocol including preoperative education, postoperative abdominal massage and polyethylene glycol 4,000 can effectively relieve constipation after THA without sacrificing safety.


Assuntos
Artroplastia de Quadril/efeitos adversos , Constipação Intestinal/prevenção & controle , Massagem/enfermagem , Polietilenoglicóis/uso terapêutico , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Cuidados Pré-Operatórios/métodos , Resultado do Tratamento
17.
Preprint | bioRxiv | ID: ppbiorxiv-000885

RESUMO

COVID-19 has become a global pandemic caused by a novel coronavirus SARS-CoV-2. Understanding the origins of SARS-CoV-2 is critical for deterring future zoonosis and for drug discovery and vaccine development. We show evidence of strong purifying selection around the receptor binding motif (RBM) in the spike gene and in other genes among bat, pangolin and human coronaviruses, indicating similar strong evolutionary constraints in different host species. We also demonstrate that SARS-CoV-2s entire RBM was introduced through recombination with coronaviruses from pangolins, possibly a critical step in the evolution of SARS-CoV-2s ability to infect humans. Similar purifying selection in different host species and frequent recombination among coronaviruses suggest a common evolutionary mechanism that could lead to new emerging human coronaviruses. One Sentence SummaryExtensive Recombination and Strong Purifying Selection among coronaviruses from different hosts facilitate the emergence of SARS-CoV-2

18.
Preprint | medRxiv | ID: ppmedrxiv-20045757

RESUMO

BackgroundThe first case of COVID-19 atypical pneumonia was reported in Wuhan, China on December 1, 2019. Since then, at least 33 other countries have been affected and there is a possibility of a global outbreak. A tremendous amount of effort has been made to understand its transmission dynamics; however, the temporal and spatial transmission heterogeneity and changing epidemiology have been mostly ignored. The epidemic mechanism of COVID-19 remains largely unclear. MethodsEpidemiological data on COVID-19 in China and daily population movement data from Wuhan to other cities were obtained and analyzed. To describe the transmission dynamics of COVID-19 at different spatio-temporal scales, we used a three-stage continuous-time Susceptible-Exposed-Infectious-Recovered (SEIR) meta-population model based on the characteristics and transmission dynamics of each stage: 1) local epidemic from December 1, 2019 to January 9, 2020; 2) long-distance spread due to the Spring Festival travel rush from January 10 to 22, 2020; and 3) intra-provincial transmission from January 23, 2020 when travel restrictions were imposed. Together with the basic reproduction number (R0) for mathematical modelling, we also considered the variation in infectivity and introduced the controlled reproduction number (Rc) by assuming that exposed individuals to be infectious; we then simulated the future spread of COVID across Wuhan and all the provinces in mainland China. In addition, we built a novel source tracing algorithm to infer the initial exposed number of individuals in Wuhan on January 10, 2020, to estimate the number of infections early during this epidemic. FindingsThe spatial patterns of disease spread were heterogeneous. The estimated controlled reproduction number (Rc) in the neighboring provinces of Hubei province were relatively large, and the nationwide reproduction number - except for Hubei - ranged from 0.98 to 2.74 with an average of 1.79 (95% CI 1.77-1.80). Infectivity was significantly greater for exposed than infectious individuals, and exposed individuals were predicted to have become the major source of infection after January 23. For the epidemic process, most provinces reached their epidemic peak before February 10, 2020. It is expected that the maximum number of infections will be approached by the end of March. The final infectious size is estimated to be about 58,000 for Wuhan, 20,800 for the rest of Hubei province, and 17,000 for the other provinces in mainland China. Moreover, the estimated number of the exposed individuals is much greater than the officially reported number of infectious individuals in Wuhan on January 10, 2020. InterpretationThe transmission dynamics of COVID-19 have been changing over time and were heterogeneous across regions. There was a substantial underestimation of the number of exposed individuals in Wuhan early in the epidemic, and the Spring Festival travel rush played an important role in enhancing and accelerating the spread of COVID-19. However, Chinas unprecedented large-scale travel restrictions quickly reduced Rc. The next challenge for the control of COVID-19 will be the second great population movement brought by removing these travel restrictions.

19.
Preprint | medRxiv | ID: ppmedrxiv-20044099

RESUMO

COVID-19 has become a pandemic. The influence of meteorological factors on the transmission and spread of COVID-19 if of interest. This study sought to examine the associations of daily average temperature (AT) and relative humidity (ARH) with the daily count of COVID-19 cases in 30 Chinese provinces (in Hubei from December 1, 2019 to February 11, 2020 and in other provinces from January 20, 2020 to Februarys 11, 2020). A Generalized Additive Model (GAM) was fitted to quantify the province-specific associations between meteorological variables and the daily cases of COVID-19 during the study periods. In the model, the 14-day exponential moving averages (EMAs) of AT and ARH, and their interaction were included with time trend and health-seeking behavior adjusted. Their spatial distributions were visualized. AT and ARH showed significantly negative associations with COVID-19 with a significant interaction between them (0.04, 95% confidence interval: 0.004-0.07) in Hubei. Every 1{degrees}C increase in the AT led to a decrease in the daily confirmed cases by 36% to 57% when ARH was in the range from 67% to 85.5%. Every 1% increase in ARH led to a decrease in the daily confirmed cases by 11% to 22% when AT was in the range from 5.04{degrees}C to 8.2{degrees}C. However, these associations were not consistent throughout Mainland China.

20.
Cancer Res ; 80(5): 1049-1063, 2020 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31888886

RESUMO

Wnt/ß-catenin signaling activates the transcription of target genes to regulate stem cells and cancer development. However, the contribution of epigenetic regulation to this process is unknown. Here, we report that Wnt activation stabilizes the epigenetic regulator KDM4C that promotes tumorigenesis and survival of human glioblastoma cells by epigenetically activating the transcription of Wnt target genes. KDM4C protein expression was upregulated in human glioblastomas, and its expression directly correlated with Wnt activity and Wnt target gene expression. KDM4C was essential for Wnt-induced gene expression and tumorigenesis of glioblastoma cells. In the absence of Wnt3a, protein kinase R phosphorylated KDM4C at Ser918, inducing KDM4C ubiquitination and degradation. Wnt3a stabilized KDM4C through inhibition of GSK3-dependent protein kinase R activity. Stabilized KDM4C accumulated in the nucleus and bound to and demethylated TCF4-associated histone H3K9 by interacting with ß-catenin, promoting HP1γ removal and transcriptional activation. These findings reveal that Wnt-KDM4C-ß-catenin signaling represents a novel mechanism for the transcription of Wnt target genes and regulation of tumorigenesis, with important clinical implications. SIGNIFICANCE: These findings identify the Wnt-KDM4C-ß-catenin signaling axis as a critical mechanism for glioma tumorigenesis that may serve as a new therapeutic target in glioblastoma.


Assuntos
Neoplasias Encefálicas/genética , Carcinogênese/genética , Epigênese Genética , Glioblastoma/genética , Histona Desmetilases com o Domínio Jumonji/metabolismo , Proteínas Wnt/metabolismo , Neoplasias Encefálicas/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , Desmetilação do DNA , Regulação Neoplásica da Expressão Gênica , Glioblastoma/patologia , Histonas/genética , Histonas/metabolismo , Humanos , Estabilidade Proteica , Fator de Transcrição 4/genética , Transcrição Genética , Ubiquitinação/genética , Via de Sinalização Wnt/genética , beta Catenina/genética , beta Catenina/metabolismo
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