Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 57
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Agric Food Chem ; 67(22): 6275-6284, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-31083910

RESUMO

Many O-glucuronides exhibiting various pharmacological activities have been found in nature and in drug metabolism. The glucuronidation of bioactive natural products or drugs to generate glucuronides with better activity and druggability is important in drug discovery and research. In this study, by using two uridine diphosphate (UDP)-dependent glucuronosyltransferases (GATs, UGT88D4 and UGT88D7) from plants, we developed two glucuronidation approaches, pure enzyme catalysis in vitro and recombinant whole-cell catalysis in vivo, to efficiently synthesize bioactive O-glucuronides by the glucuronidation of natural products. In total, 14 O-glucuronides with different structures, including flavonoids, anthraquinones, coumarins, and lignans, were obtained, 7 of which were new compounds. Furthermore, one of the biosynthesized O-glucuronides, kaempferol-7- O-ß-d-glucuronide (3a), potently inhibited protein tyrosine phosphatase (PTP) 1B with an IC50 value of 8.02 × 10-6 M. Some of the biosynthesized O-glucuronides also exhibited significant antioxidant activities.


Assuntos
Antirrhinum/enzimologia , Glucuronídeos/química , Glucuronosiltransferase/química , Perilla frutescens/enzimologia , Proteínas de Plantas/química , Antirrhinum/genética , Biocatálise , Estabilidade Enzimática , Glucuronosiltransferase/genética , Glucuronosiltransferase/metabolismo , Cinética , Estrutura Molecular , Perilla frutescens/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Especificidade por Substrato
2.
Polymers (Basel) ; 11(4)2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30960574

RESUMO

This study shows the feasibility of using electrospinning technique to prepare polytetrafluoroethylene/poly (vinyl alcohol) (PTFE/PVA) nanofibers on PTFE microfiber membrane as substrate. Then, PVA in the fiber membrane was removed by thermal treatment at about 350 °C. Compared to PTFE microfiber substrates, the composite PTFE fiber membranes (CPFMs) have improved filtration efficiency by 70% and water contact angle by 23°. Experimental test data showed that the water contact angle of the sample increased from about 107° to 130°, the filtration efficiency of PM2.5 increased from 44.778% to 98.905%, and the filtration efficiency of PM7.25 increased from 66.655% to 100% due to the electrospun PTFE nanofiber layer. This work demonstrates the potential of CPFMs as a filter for the production of indoor or outdoor dust removal and industrially relevant gas filtration.

3.
Int J Cancer ; 145(10): 2712-2719, 2019 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-30989655

RESUMO

The development of highly sensitive HPV-genotyping tests has opened the possibility of treating HPV-infected women before high-grade lesions appear. The lack of efficient intervention for persistent high-risk HPV infection necessitates the need for development of novel therapeutic strategy. Here we demonstrate that REBACIN®, a proprietary antiviral biologics, has shown potent efficacy in the clearance of persistent HPV infections. Two independent parallel clinical studies were investigated, which a total of 199 patients were enrolled and randomly divided into a REBACIN®-test group and a control group without treatment. The viral clearance rates for the REBACIN® groups were 61.5% (24/39) and 62.5% (35/56), respectively, for the two independent parallel studies. In contrast, the nontreatment groups showed self-clearance rates at 20.0% (8/40) and 12.5% (8/64). We further found that REBACIN® was able to significantly repress the expression of HPV E6 and E7 oncogenes in TC-1 and Hela cells. The two viral genes are well known for the development of high-grade premalignancy lesion and cervical cancer. In a mouse model, REBACIN® was indicated to notably suppress E6/E7-induced tumor growth, suggesting E6 and E7 oncogenes as a potential target of REBACIN®. Taken together, our studies shed light into the development of a novel noninvasive therapeutic intervention for clearance of persistent HPV infection with significant efficacy.


Assuntos
Antivirais/uso terapêutico , Produtos Biológicos/uso terapêutico , Infecções por Papillomavirus/tratamento farmacológico , Neoplasias do Colo do Útero/prevenção & controle , Adulto , Animais , Antivirais/farmacologia , Produtos Biológicos/farmacologia , Modelos Animais de Doenças , Feminino , Células HeLa , Papillomavirus Humano 16/efeitos dos fármacos , Papillomavirus Humano 16/patogenicidade , Humanos , Camundongos , Pessoa de Meia-Idade , Proteínas Oncogênicas Virais/antagonistas & inibidores , Proteínas E7 de Papillomavirus/antagonistas & inibidores , Infecções por Papillomavirus/virologia , Proteínas Repressoras/antagonistas & inibidores , Resultado do Tratamento , Neoplasias do Colo do Útero/virologia , Carga Viral/efeitos dos fármacos
4.
Angew Chem Int Ed Engl ; 58(2): 618-623, 2019 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-30461146

RESUMO

The precise synthesis of poly(thioester)s with diverse structures is still a significant challenge in the polymeric materials field. Herein, we report a novel approach to the synthesis of well-defined poly(thioester)s by the controlled alternating copolymerization of cyclic thioanhydrides and episulfides induced by simple organic ammonium salts. Both the cation and anion have strong effects on the copolymerization. [PPN]OAc ([PPN]=bis(triphenylphosphine)iminium) with a bulky cation was proven to be efficient in initiating this polymerization, yielding poly(thioester)s with a completely alternating structure, controlled molecular weight, and narrow polydispersity. The poly(thioester) obtained from succinic thioanhydride and propylene sulfide is a typical semicrystalline material, possessing a high refractive index of up to 1.78. Because it uses readily available monomers, this method is expected to open up a new route to poly(thioester)s with diverse structures and properties.

5.
Appl Microbiol Biotechnol ; 103(2): 893-902, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30421106

RESUMO

It is generally considered that bile salt hydrolase (BSH) activity is hardly detected in nonintestinal lactic acid bacteria (LAB). The aim of this study was to investigate the distribution and intensity of BSH activity in LAB isolated from naturally fermented vegetables and milk. A total of 624 lactic acid bacterial strains classified into 6 genera and 50 species were isolated from 144 naturally fermented vegetable samples and 103 naturally fermented milk samples, and their BSH activity was screened by gas chromatography with electron capture detection. The BSH-positive strains were further analyzed quantitatively for their deconjugation ability against six human-conjugated bile salts by HPLC based on the disappearance of the conjugated bile salts from the reaction mixture. The results showed that 39% of the strains possessed BSH activity distributed in 24 lactic acid bacterial species. The strains of the fermented vegetable origin showed a 0.5-fold higher incidence of BSH-positive strains than those of the fermented milk origin, and the lactic acid bacilli exhibited 2.5-fold higher incidence of BSH-positive strains than the lactic acid cocci in general. The strains of the fermented vegetable origin generally had greater bile salt deconjugation ability than those of the fermented milk origin. More than 97% and 93% of the BSH-positive strains exhibited a greater substrate preference for glycoconjugated bile salts than tauroconjugated bile salts and for dihydroxy bile salts than trihydroxy bile salts, respectively. This study demonstrated that BSH activity was also present in nonintestinal LAB.


Assuntos
Amidoidrolases/análise , Lactobacillales/enzimologia , Ácidos e Sais Biliares/metabolismo , Laticínios/microbiologia , Hidrólise , Lactobacillales/classificação , Lactobacillales/isolamento & purificação , Verduras/microbiologia
6.
Mol Nutr Food Res ; 62(24): e1800728, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30346664

RESUMO

SCOPE: Lactobacillus casei F0822-fermented milk has exhibited significant hypocholesterolemic activity in hamsters in the previous study. Under this premise, the objective of this study is to further explore whether bile salt hydrolase (BSH) and S-layer protein (SLP) of the strain have a significant influence on hypocholesterolemic activity of the fermented milk. METHODS AND RESULTS: Independent and double interposon mutants of BSH and SLP genes are constructed from wild-type L. casei F0822 via chromosomal insertion of chloramphenicol or/and erythromycin resistance genes based on double-crossover homologous recombination. The mutants- and the wild-type strain-fermented milk is prepared (viable counts of approximately 8.0 × 108 colony-forming units mL-1 each) and intragastrically administered to high-cholesterol-fed hamsters once daily at a dose of 1.25 mL d-1 for 28 d, respectively. Both the BSH-deficient mutant- and the SLP-deficient mutant-fermented milk significantly (p < 0.05) increase serum total and LDL-cholesterol levels in hamsters compared with the wild-type strain-fermented milk. However, only the BSH-deficient mutant-fermented milk could significantly (p < 0.05) increase hepatic total and esterified cholesterol levels in hamsters. CONCLUSION: Both BSH and SLP have a significant influence on the hypocholesterolemic activity of L. casei F0822-fermented milk in hamsters. Nevertheless, the BSH is greater than the SLP in this regard.


Assuntos
Amidoidrolases/farmacologia , Anticolesterolemiantes/farmacologia , Produtos Fermentados do Leite , Lactobacillus casei/química , Glicoproteínas de Membrana/farmacologia , Amidoidrolases/genética , Animais , Proteínas de Bactérias/farmacologia , Ácidos e Sais Biliares/metabolismo , Colesterol/sangue , Colesterol/genética , Colesterol/metabolismo , Fezes/química , Regulação da Expressão Gênica , Lactobacillus casei/genética , Fígado/metabolismo , Masculino , Glicoproteínas de Membrana/genética , Mesocricetus , Mutação
7.
Nanoscale Res Lett ; 13(1): 239, 2018 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-30112698

RESUMO

Electrospinning provides an effective method for generating nanofibers from solution of carboxymethyl chitosan/polyoxyethylene oxide (CMCS/PEO). The goal of this work is to explore the potential application of electrospun CMCS/PEO nanofiber membrane in fruit fresh-keeping. The microstructure, antibacterial activity, hydrophilia, and air permeability of the nanofiber membrane have been tested. For comparison, the fresh-keeping effects of commercial cling wrap and CMCS/PEO nanofiber membranes on strawberries' rotting rate and weight loss rate have been studied. The results indicate that the electrospun CMCS/PEO membrane could effectively avoid water loss in strawberries and has a remarkable effect to prolong strawberries' shelf life due to its breathability and antibacterial activity. In addition, the composite CMCS/PEO, nanofiber membrane is non-poisonous and edible, which can be used in food industry.

8.
Angew Chem Int Ed Engl ; 57(39): 12670-12674, 2018 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-30088310

RESUMO

Synthesis of chiral sulfur-containing polymers was realized for the first time by the asymmetric alternating copolymerization of achiral meso-epoxides with carbonyl sulfide (COS) using catalyst systems based on enantiopure binaphthol-linked dinuclear CoIII complexes under mild reaction conditions. The resultant poly(monothiocarbonate)s have main-chain chirality and more than 99 % isotacticity. Notably, the stereoregular copolymers are typical semicrystalline thermoplastics with high melting temperatures up to 232 °C. Additionally, these sulfur-containing polymers have good optical properties with refractive indices of up to 1.56 and Abbe's numbers of up to 43.

9.
J Asian Nat Prod Res ; 20(7): 605-614, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29989425

RESUMO

Glucuronidation is an important and popular metabolic reaction in vivo of drugs. The further evaluation of biological activity and toxicity of glucuronides is necessary in the course of the drug research and development. However, the synthesis of glucuronides is limited by the lack of efficient approach. Herein, we have developed a new glucuronide synthesis method using plant uridine diphosphate-dependent glucuronosyltransferases (UGTs), UGT88D4, UGT88D7, and EpGT8, enabling the convenient preparation for corresponding O-glucuronide metabolites (1a, 2a, 3a, and 3b) in milligram scale of two neurological active agents, IMM-H004 (1) and FLZ (2). Their structures were characterized by spectroscopic data analyses.


Assuntos
Glucuronídeos/síntese química , Glucuronosiltransferase/metabolismo , Plantas/enzimologia , Clonagem Molecular , Glucuronosiltransferase/química , Glucuronosiltransferase/genética , Concentração de Íons de Hidrogênio , Espectroscopia de Ressonância Magnética , Metais/química , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray , Temperatura Ambiente
10.
Mol Nutr Food Res ; 62(16): e1800170, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29939474

RESUMO

SCOPE: Rats and hamsters are the most commonly used animal models for evaluating the hypocholesterolemic activity of potential probiotic strains, whereas little or no information has been reported on whether the animal models would affect the experimental conclusions regarding the hypocholesterolemic efficacy of the strains. METHODS AND RESULTS: Both high-cholesterol-fed rats and hamsters were intragastrically administered viable cells of bile salt hydrolase-active Lactobacillus acidophilus K16 once daily (1 × 1010 CFU per kg body weight) for 28 d. It was found that the strain did not significantly (p > 0.05) affect the serum and hepatic cholesterol levels in rats, whereas it significantly decreased (p < 0.01 or p < 0.001) the serum total and non-HDL-cholesterol as well as hepatic-free, esterified, and total cholesterol levels in hamsters by 29.6%, 38.8%, 15.8%, 36.2%, and 34.0%, respectively. CONCLUSION: These data suggest that the hypocholesterolemic efficacy of L. acidophilus K16 is substantially different between high-cholesterol-fed hamsters and rats and that hamsters are a better model system than rats for evaluating the hypocholesterolemic efficacy of potential probiotic strains due to their similarity to humans in biliary bile acid composition, including types of bile acids and their conjugation form.


Assuntos
Hipercolesterolemia/tratamento farmacológico , Lactobacillus acidophilus , Probióticos/uso terapêutico , Animais , Ácidos e Sais Biliares/análise , Colesterol/análise , Colesterol/sangue , Colesterol 7-alfa-Hidroxilase/análise , Cricetinae , Fezes/química , Fígado/metabolismo , Masculino , Mesocricetus , Modelos Animais , Ratos , Ratos Wistar , Especificidade da Espécie , Especificidade por Substrato
11.
PLoS One ; 13(3): e0192964, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29494656

RESUMO

This study aimed to analyze the bile acid patterns in commercially available oxgall powders used for evaluation of the bile tolerance ability of probiotic bacteria. Qxgall powders purchased from Sigma-Aldrich, Oxoid and BD Difco were dissolved in distilled water, and analyzed. Conjugated bile acids were profiled by ion-pair high-performance liquid chromatography (HPLC), free bile acids were detected as their p-bromophenacyl ester derivatives using reversed-phase HPLC after extraction with acetic ether, and total bile acids were analyzed by enzymatic-colorimetric assay. The results showed that 9 individual bile acids (i.e., taurocholic acid, glycocholic acid, taurodeoxycholic acid, glycodeoxycholic acid, taurochenodeoxycholic acid, glycochenodeoxycholic acid, cholic acid, chenodeoxycholic acid, deoxycholic acid) were present in each of the oxgall powders tested. The content of total bile acid among the three oxgall powders was similar; however, the relative contents of the individual bile acids among these oxgall powders were significantly different (P < 0.001). The oxgall powder from Sigma-Aldrich was closer to human bile in the ratios of glycine-conjugated bile acids to taurine-conjugated bile acids, dihydroxy bile acids to trihydroxy bile acids, and free bile acids to conjugated bile acids than the other powders were. It was concluded that the oxgall powder from Sigma-Aldrich should be used instead of those from Oxoid and BD Difco to evaluate the bile tolerance ability of probiotic bacteria as human bile model.


Assuntos
Ácidos e Sais Biliares/análise , Bile/química , Probióticos , Animais , Bovinos , Cromatografia Líquida de Alta Pressão , Humanos , Pós , Probióticos/metabolismo
12.
Org Lett ; 20(6): 1634-1637, 2018 03 16.
Artigo em Inglês | MEDLINE | ID: mdl-29470079

RESUMO

The enzymatic synthesis of coumarin C-glucosides by an engineered C-glycosyltransferase, MiCGTb-GAGM, was explored in vitro and in vivo. MiCGTb-GAGM exhibited a robust C-glucosylation capability toward structurally diverse coumarin derivatives. The whole-cell bioconversion of MiCGTb-GAGM was exploited for large-scale production of coumarin C-glucosides. Two C-glucosides exhibited potent SGLT2 inhibitory activities with IC50 values at 10-6 M. These findings provide cost-effective and practical synthetic strategies to generate structurally diverse and novel bioactive coumarin C-glycosides for drug discovery.


Assuntos
Cumarínicos/química , Glucosídeos , Glicosídeos , Glicosiltransferases , Estrutura Molecular
13.
Appl Microbiol Biotechnol ; 102(4): 1903-1910, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29330692

RESUMO

A new in vitro method was developed to determine the bile tolerance of potentially probiotic lactobacilli. The overnight culture of various lactobacilli strains was inoculated into sterile, half-strength MRS broth supplemented with and without 0.3% (wt/vol) oxgall, buffered with 0.1 M sodium phosphate buffer at a final pH of 7.3, and incubated at 37 °C for 12 h under anaerobic conditions. The bile tolerance ability of the lactobacilli strains was expressed as the percentage of the propagation generations of the bacterial cells in the presence of oxgall to those in the absence of oxgall. The bile tolerance ability of 11 strains of 8 Lactobacillus species, including 3 bile salt hydrolase (BSH)-negative strains and 8 BSH-positive strains, was analyzed using the newly developed method and two traditional methods. The results showed that bile tolerance ability of the strains was considerably different depending on the analysis method used. The newly developed method mimics the physiological environment of the human small intestine, and avoids changes in pH and bile salt composition during the incubation period, which are drawbacks of the traditional bile tolerance test methods. Therefore, the analysis method developed in this study is more suitable to screen or compare the bile tolerance ability of lactobacilli strains.


Assuntos
Ácidos e Sais Biliares/toxicidade , Tolerância a Medicamentos , Lactobacillus/efeitos dos fármacos , Testes de Sensibilidade Microbiana/métodos , Viabilidade Microbiana/efeitos dos fármacos , Probióticos , Meios de Cultura/química , Humanos , Concentração de Íons de Hidrogênio , Lactobacillus/fisiologia , Temperatura Ambiente
14.
Nutrition ; 46: 36-40, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29290353

RESUMO

OBJECTIVES: The aim of this study was to examine the occurrence of five azo food dyes-tartrazine, sunset yellow, carmoisine, allura red, and ponceau 4 R-in the food supply chain of Singapore and their effects on the in vitro synthesis of leukotriene B4 (LTB4) and F2-isoprostanes. METHODS: Trained personnel recorded the names of foods and beverages sold in a local supermarket that contained at least one of the five azo dyes. The occurrence of the azo dyes in the local food supply was computed. The synthesis of LTB4 and F2-isoprostanes from freshly isolated blood neutrophils were measured using gas chromatography-mass spectrometry. RESULTS: Of the 1681 processed food items, 194 (11.54%) contained at least one of the five azo dyes. Tartrazine was most prevalent in food and beverage products sold in Singapore, followed by allura red, sunset yellow, ponceau 4 R, and carmoisine. The five azo dyes augmented the in vitro synthesis of LTB4 and F2-isoprostanes from blood neutrophils. Tartrazine was significantly more potent in increasing LTB4 synthesis than the other dyes, which exhibited similar potencies. The five food dyes increased the formation of F2-isoprostanes from blood neutrophils at all tested concentrations. CONCLUSION: The high prevalence of azo dyes in the food supply of Singapore and their ability to elicit proinflammatory responses in vitro suggest a potential health risk to the local population.


Assuntos
Compostos Azo/efeitos adversos , Compostos Azo/análise , Análise de Alimentos , Corantes de Alimentos/efeitos adversos , Corantes de Alimentos/análise , Inflamação/induzido quimicamente , Bebidas/análise , F2-Isoprostanos/biossíntese , Alimentos , Humanos , Leucotrieno B4/biossíntese , Naftalenossulfonatos/efeitos adversos , Naftalenossulfonatos/análise , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Fatores de Risco , Singapura , Tartrazina/efeitos adversos , Tartrazina/análise
15.
Sci Total Environ ; 601-602: 1575-1590, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-28609846

RESUMO

Ground observations can capture CO2 concentrations accurately but the number of available TCCON (Total Carbon Column Observing Network) sites is too small to support a comprehensive analysis (i.e. validation) of satellite observations. Atmospheric transport models can provide continuous atmospheric CO2 concentrations in space and time, but some information is difficult to generate with model simulations. The HASM platform can model continuous column-averaged CO2 dry air mole fraction (XCO2) surface taking TCCON observations as its optimum control constraints and an atmospheric transport model as its driving field. This article presents a comparison of the satellite observations with a HASM XCO2 surface obtained by fusing TCCON measurements with GEOS-Chem model results. We first verified the accuracy of the HASM XCO2 surface using six years (2010-2015) of TCCON observations and the GEOS-Chem model XCO2 results. The validation results show that the largest MAE of bias between the HASM results and observations was 0.85ppm and the smallest MAE was only 0.39ppm. Next, we modeled the HASM XCO2 surface by fusing the TCCON measurements and GEOS-Chem XCO2 model results for the period 9/1/14 to 8/31/15. Finally, we compared the GOSAT and OCO-2 observations with the HASM XCO2 surface and found that the global OCO-2 XCO2 estimates more closely resembled the HASM XCO2 surface than the GOSAT XCO2 estimates.

16.
Polymers (Basel) ; 9(10)2017 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-30965818

RESUMO

An iron(III) complex of tetradentate N,N'-disubstituted bis(aminophenoxide) (designated as salan, a saturated version of the corresponding salen ligand) with a sterically hindered organic base anchored on the ligand framework, can selectively mediate the conversion of carbonyl sulfide to sulfur-containing polymers by the copolymerization with epoxides. This single-site catalyst exhibits broad substrate scope, and the resultant copolymers have completely alternating structures. In addition, this catalyst is efficient in producing diblock copolymers, suggesting a living polymerization nature.

17.
Sensors (Basel) ; 16(11)2016 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-27809272

RESUMO

The TanSat carbon satellite is to be launched at the end of 2016. In order to verify the performance of its instruments, a flight test of TanSat instruments was conducted in Jilin Province in September, 2015. The flight test area covered a total area of about 11,000 km² and the underlying surface cover included several lakes, forest land, grassland, wetland, farmland, a thermal power plant and numerous cities and villages. We modeled the column-average dry-air mole fraction of atmospheric carbon dioxide (XCO2) surface based on flight test data which measured the near- and short-wave infrared (NIR) reflected solar radiation in the absorption bands at around 760 and 1610 nm. However, it is difficult to directly analyze the spatial distribution of XCO2 in the flight area using the limited flight test data and the approximate surface of XCO2, which was obtained by regression modeling, which is not very accurate either. We therefore used the high accuracy surface modeling (HASM) platform to fill the gaps where there is no information on XCO2 in the flight test area, which takes the approximate surface of XCO2 as its driving field and the XCO2 observations retrieved from the flight test as its optimum control constraints. High accuracy surfaces of XCO2 were constructed with HASM based on the flight's observations. The results showed that the mean XCO2 in the flight test area is about 400 ppm and that XCO2 over urban areas is much higher than in other places. Compared with OCO-2's XCO2, the mean difference is 0.7 ppm and the standard deviation is 0.95 ppm. Therefore, the modelling of the XCO2 surface based on the flight test of the TanSat instruments fell within an expected and acceptable range.

18.
Int J Food Microbiol ; 233: 44-51, 2016 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-27322723

RESUMO

Zygosaccharomyces rouxii is a yeast which can cause spoilage in the concentrated juice industries. It exhibits resistance to high sugar concentrations but genome- and proteome-wide studies on Z. rouxii in response to high sugar concentrations have been poorly investigated. Herein, by using a 2-D electrophoresis based workflow, the proteome of a wild strain of Z. rouxii under different sugar concentrations has been analyzed. Proteins were extracted, quantified, and subjected to 2-DE analysis in the pH range 4-7. Differences in growth (lag phase), protein content (13.97-19.23mg/g cell dry weight) and number of resolved spots (196-296) were found between sugar concentrations. ANOVA test showed that 168 spots were different, and 47 spots, corresponding to 40 unique gene products have been identified. These protein species are involved in carbohydrate and energy metabolism, amino acid metabolism, response to stimulus, protein transport and vesicle organization, cell morphogenesis regulation, transcription and translation, nucleotide metabolism, amino-sugar nucleotide-sugar pathways, oxidoreductases balancing, and ribosome biogenesis. The present study provides important information about how Z. rouxii acts to cope with high sugar concentration at molecular levels, which might enhance our global understanding of Z. rouxii's high sugar-tolerance trait.


Assuntos
Carboidratos/análise , Proteínas Fúngicas/análise , Zygosaccharomyces/metabolismo , Metabolismo dos Carboidratos , Meios de Cultura/química , Meios de Cultura/metabolismo , Proteínas Fúngicas/metabolismo , Zygosaccharomyces/química , Zygosaccharomyces/crescimento & desenvolvimento
19.
Sci Total Environ ; 543(Pt A): 609-619, 2016 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-26613514

RESUMO

Large numbers of livestock and poultry feces are continuously applied into soils in intensive vegetable cultivation areas, and then some veterinary antibiotics are persistent existed in soils and cause health risk. For the spatial heterogeneity of antibiotic residues, developing a suitable technique to interpolate soil antibiotic residues is still a challenge. In this study, we developed an effective interpolator, high accuracy surface modeling (HASM) combined vegetable types, to predict the spatial patterns of soil antibiotics, using 100 surface soil samples collected from an intensive vegetable cultivation area located in east of China, and the fluoroquinolones (FQs), including ciprofloxacin (CFX), enrofloxacin (EFX) and norfloxacin (NFX), were analyzed as the target antibiotics. The results show that vegetable type is an effective factor to be combined to improve the interpolator performance. HASM achieves less mean absolute errors (MAEs) and root mean square errors (RMSEs) for total FQs (NFX+CFX+EFX), NFX, CFX and EFX than kriging with external drift (KED), stratified kriging (StK), ordinary kriging (OK) and inverse distance weighting (IDW). The MAE of HASM for FQs is 55.1 µg/kg, and the MAEs of KED, StK, OK and IDW are 99.0 µg/kg, 102.8 µg/kg, 106.3 µg/kg and 108.7 µg/kg, respectively. Further, RMSE simulated by HASM for FQs (CFX, EFX and NFX) are 106.2 µg/kg (88.6 µg/kg, 20.4 µg/kg and 39.2 µg/kg), and less 30% (27%, 22% and 36%), 33% (27%, 27% and 43%), 38% (34%, 23% and 41%) and 42% (32%, 35% and 51%) than the ones by KED, StK, OK and IDW, respectively. HASM also provides better maps with more details and more consistent maximum and minimum values of soil antibiotics compared with the measured data. The better performance can be concluded that HASM takes the vegetable type information as global approximate information, and takes local sampling data as its optimum control constraints.


Assuntos
Antibacterianos/análise , Monitoramento Ambiental/métodos , Modelos Químicos , Poluentes do Solo/análise , Solo/química , China , Análise Espacial
20.
Ying Yong Sheng Tai Xue Bao ; 27(11): 3455-3462, 2016 Nov 18.
Artigo em Chinês | MEDLINE | ID: mdl-29696841

RESUMO

In order to elucidate the effects of intensive management on soil carbon pool, nitrogen pool, enzyme activities in Moso bamboo (Phyllostachys pubescens) plantations, we collected soil samples from the soil surface (0-20 cm) and subsurface (20-40 cm) layers in the adjacent Moso bamboo plantations with extensive and intensive managements in Sankou Township, Lin'an City, Zhejiang Province. We determined different forms of C, N and soil invertase, urease, catalase and acid phosphatase activities. The results showed that long-term intensive management of Moso bamboo plantations significantly decreased the content and storage of soil organic carbon (SOC), with the SOC storage in the soil surface and subsurface layers decreased by 13.2% and 18.0%, respectively. After 15 years' intensive management of Masoo bamboo plantations, the contents of soil water soluble carbon (WSOC), hot water soluble carbon (HWSOC), microbial carbon (MBC) and readily oxidizable carbon (ROC) were significantly decreased in the soil surface and subsurface layers. The soil N storage in the soil surface and subsurface layers in intensively managed Moso bamboo plantations increased by 50.8% and 36.6%, respectively. Intensive management significantly increased the contents of nitrate-N (NO3--N) and ammonium-N (NH4+-N), but decreased the contents of water-soluble nitrogen (WSON) and microbial biomass nitrogen (MBN). After 15 years' intensive management of Masoo bamboo plantations, the soil invertase, urease, catalase and acid phosphatase activities in the soil surface layer were significantly decreased, the soil acid phosphatase activity in the soil subsurface layer were significantly decreased, and other enzyme activities in the soil subsurface layer did not change. In conclusion, long-term intensive management led to a significant decline of soil organic carbon storage, soil labile carbon and microbial activity in Moso bamboo plantations. Therefore, we should consider the use of organic fertilizer in the intensive mana-gement process for the sustainable management of Moso bamboo plantations in the future.


Assuntos
Carbono/química , Nitrogênio/química , Poaceae/crescimento & desenvolvimento , Solo/química , Fosfatase Ácida/metabolismo , Biomassa , Catalase/metabolismo , China , Urease/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA