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1.
PLoS One ; 15(11): e0241572, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33147295

RESUMO

INTRODUCTION: Neurosyphilis can occur at any stage of syphilis. After treatment, 30%-40% of syphilis patients remained serofast. But the prevalence of asymptomatic neurosyphilis (ANS) among serofast syphilis patients remains unclear. Untimely treatment or improper management for ANS may result in neurological complications. So we perform the meta-analysis to evaluate the prevalence of ANS cases among HIV-negative serofast syphilis patients for exploring their relationship and addressing their clinical management. METHODS: We searched CNKI, Wan Fang, VIP, CBMdisc, PubMed, Embase and Medline from January 1st 1990 to September 22nd 2020 for both English and Chinese records. We strictly restrict the eligibility criteria. STROBE was used for reporting quality assessment. We examined forest plots and conducted both fix-effects and random-effects to estimate prevalence by R version 3.6.2/R studio 1.2.1335 statistical software packages META version 4.9-9. If appropriate, between-study heterogeneity was examined using the I2 statistic and subgroup analysis. RESULTS: Of 77 screened records, 5 were included. The pooled prevalence of ANS among HIV-negative serofast syphilis patients was 13% (95% CI 3%-23%; I2 = 93% P<0.01, 417 people). The prevalence of ANS for the verified ANS classification definition was 3% (95% CI 0%-7%; I2 = 67% P = 0.08, two studies, 189 people), and 21% (95% CI 6%-36%; I2 = 86% P<0.01, three studies, 228 people) for the likely ANS classification. The prevalence of ANS among the serofast syphilis patients who were followed up for one year was 29% (95% CI 22%-36%; I2 = 0% P = 0.5, two studies, 167 people) and 5% (95% CI 0%-13%; I2 = 79% P = 0.03, two studies, 144 people) for two years. The prevalence in the studies from different geographical subgroups was as follows: 9% (95% CI 0%-19%; I2 = 82% P<0.01, three studies, 169 people) in South-central China, 6% (95% CI 1%-10%; one study, 106 people) in East China, and 30% (95% CI 23%-38%; one study, 142 people) in North China. CONCLUSION: This meta-analysis showed a high estimated prevalence of ANS in HIV-negative serofast syphilis patients, the prevalence of ANS among patients diagnosed with the verified ANS case definition is much lower than that for the likely ANS classification. It may be necessary to carry out nontreponemal test, protein test and leukocyte count for cerebrospinal fluid (CSF) in treated serofast patients for better clinical management to avoid neurological complications. The case classification definition of ANS is a key factor to evaluate the prevalence. Geographical heterogeneity needs more studies to detect. In future we need better-design studies to explore relationship between ANS and serofast status.

2.
Sex Transm Dis ; 47(1): 28-33, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31651711

RESUMO

BACKGROUND: Data are scarce on sexually transmitted infection (STI) treatment-seeking behavior among men who have sex with men (MSM) in China. The purpose of the study was to characterize the first response to STI symptoms, treatment-seeking behavior, and its associated factors among MSM in China. METHODS: A cross-sectional study with convenience sampling design was conducted in 13 cities from April to July 2017. Face-to-face interviews were conducted to collect information on demographics, STI symptoms, and treatment-seeking behavior. Logistic regression analysis was conducted to evaluate variables associated with not seeking treatment. RESULTS: Among 3925 recruited participants, 628 (16.0%) reported STI symptoms in the past 12 months and were included in the analysis. Upon or after being notified of the symptoms, MSM sought help from various sources or self-tested using rapid testing kits. Only 322(51.3%) went to see a doctor in clinic. In multiple logistic regression analyses, younger age, lower income, less STI knowledge (adjusted odds ratio [AOR], 1.8; 95% confidence interval [CI], 1.2-2.5), not knowing own STI risk (AOR, 1.8; 95% CI, 1.2-2.7), no symptoms of anogenital ulcers (AOR, 2.3; 95% CI, 1.3-3.8) or anogenital warts (AOR, 2.4; 95% CI, 1.6-3.7), no syphilis testing in the past 12 months (AOR, 2.1; 95% CI, 1.5-3.0) were associated with higher probability of not seeking treatment in clinics. CONCLUSIONS: We found a high prevalence of STI symptoms among MSM and only half sought treatment. Prevention efforts should promote STI education, testing, and adequate treatment-seeking behavior.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Serviços de Saúde/estatística & dados numéricos , Aceitação pelo Paciente de Cuidados de Saúde , Minorias Sexuais e de Gênero , Doenças Sexualmente Transmissíveis/diagnóstico , Doenças Sexualmente Transmissíveis/epidemiologia , Adolescente , Adulto , China/epidemiologia , Cidades/epidemiologia , Estudos Transversais , Comportamentos Relacionados com a Saúde , Homossexualidade Masculina , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Análise de Regressão , Assunção de Riscos , Comportamento Sexual , Parceiros Sexuais , Doenças Sexualmente Transmissíveis/tratamento farmacológico , Inquéritos e Questionários , Adulto Jovem
3.
Int J Ophthalmol ; 12(4): 529-535, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31024802

RESUMO

AIM: To identify the pathogenic genes in pterygium. METHODS: We obtained mRNA expression profiles from the Gene Expression Omnibus database (GEO) to identify differentially expressed genes (DEGs) between pterygium tissues and normal conjunctiva tissues. The Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, protein-protein interaction (PPI) network and transcription factors (TFs)-target gene regulatory network was performed to understand the function of DEGs. The expression of selected DEGs were validated by the quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: A total of 557 DEGs were identified between pterygium and normal individual. In PPI network, several genes were with high degrees such as FN1, KPNB1, DDB1, NF2 and BUB3. SSH1, PRSS23, LRP5L, MEOX1, RBM14, ABCA1, JOSD1, KRT6A and UPK1B were the most downstream genes regulated by TFs. qRT-PCR results showed that FN1, PRSS23, ABCA1, KRT6A, ECT2 and SPARC were significantly up-regulated in pterygium and MEOX1 and MMP3 were also up-regulated with no significance, which was consistent with the our integrated analysis. CONCLUSION: The deregulated genes might be involved in the pathology of pterygium and could be used as treatment targets for pterygium.

4.
Int J Oncol ; 54(4): 1387-1397, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30720048

RESUMO

Breast cancer is one of the most common types of cancers which results in a high mortality rate for patients worldwide. In this study, we performed systematical experiments including tissue analysis (immunohistochemistry etc.) and cell functional experiments (cell counting assay, MTT assay, cell colony formation, cell migration assay, cell invasion assay etc.). We demonstrated that the expression level of RNA binding motif protein 3 (RBM3) was higher in human breast cancer tissues compared with adjacent non­tumor tissues. A high level of RBM3 was associated with worse post­operative relapse­free survival (RFS) and overall survival (OS) rates in patients with breast cancer. Among the patients with breast cancer, the expression of RBM3 was associated with patient lymph node metastasis and a high tumor grade. The knockdown of RBM3 markedly decreased the proliferation and metastasis of human breast cancer cells. In downstream pathway analysis, actin related protein 2/3 complex subunit 2 (ARPC2) was determined to be positively regulated by RBM3 through a post­transcriptional 3'UTR­binding manner. ARPC2 also played an oncogenic role and mediated the promoting role of RBM3 in the proliferation and metastasis of human breast cancer cells. Thus, on the whole, the findings of this study demonstrate that RBM3 acts as an oncogene in human breast cancer cells and that the functional depletion of RBM3 may be considered as a potential method for breast cancer therapy.


Assuntos
Complexo 2-3 de Proteínas Relacionadas à Actina/genética , Neoplasias da Mama/metabolismo , Proteínas de Ligação a RNA/metabolismo , Regulação para Cima , Regiões 3' não Traduzidas , Complexo 2-3 de Proteínas Relacionadas à Actina/metabolismo , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Proliferação de Células , Progressão da Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Células MCF-7 , Gradação de Tumores , Metástase Neoplásica , Análise de Sobrevida
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 193: 415-421, 2018 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-29277072

RESUMO

In this paper, a simple naphthalene-based derivative (HL) has been designed and synthesized as a Al3+-selective fluorescent chemosensor based on the PET mechanism. HL exhibited high selectivity and sensitivity towards Al3+ over other commonly coexisting metal ions in ethanol with a detection limit of 2.72nM. The 1:1 binding stoichiometry of the complex (HL-Al3+) was determined from the Job's plot based on fluorescence titrations and the ESI-MS spectrum data. Moreover, the binding site of HL with Al3+ was assured by the 1H NMR titration experiment. The binding constant (Ka) of the complex (HL-Al3+) was calculated to be 5.06×104M-1 according to the Benesi-Hildebrand equation. In addition, the recognizing process of HL towards Al3+ was chemically reversible by adding Na2EDTA. Importantly, HL could directly and rapidly detect aluminum ion through the filter paper without resorting to additional instrumental analysis.

6.
Biomed Res Int ; 2017: 2093421, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28638824

RESUMO

OBJECTIVES: To evaluate club drug use and its potential association with STI among female sex workers (FSWs) in China. METHODS: From November 2008 to January 2009, participants were recruited at sex work venues in five cities for a questionnaire survey. Free testing for syphilis, Chlamydia trachomatis (CT), and Neisseria gonorrhoeae (NG) was provided. Logistic regression models were used to assess factors associated with club drug use and its association with STI. RESULTS: A total of 1604 eligible FSWs were included. The overall prevalence of any STI infection and club drug use in the past 12 months was 22.6% and 7.4%, respectively. STI prevalence was significantly higher among club drug users (33.1%) than among nonusers (21.7%, P < 0.05). Multivariable logistic regression found that club drug use was associated with younger age (AOR 2.4, 95% CI 1.0, 6.0), higher education, having injected drugs (AOR 24.4, 95% CI 6.2, 96.8), and having had STI symptoms (AOR 2.2, 95% CI 1.4, 3.4). CONCLUSIONS: Club drug use and STI were highly prevalent among FSWs in China, especially among young FSWs. Club drug users had more risk behaviors and higher STI rates. A coordinated risk reduction framework is urgently needed to address the dual epidemic of drug use and STI.


Assuntos
Profissionais do Sexo , Doenças Sexualmente Transmissíveis/epidemiologia , Transtornos Relacionados ao Uso de Substâncias/epidemiologia , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático , China/epidemiologia , Feminino , Humanos , Prevalência
7.
Zhonghua Liu Xing Bing Xue Za Zhi ; 37(3): 358-61, 2016 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-27005536

RESUMO

OBJECTIVE: To understand the performance of HIV detection, prevalence and related factors in sexually transmitted diseases (STD) clinics and provide evidence for the improvement of HIV test and counsel services. METHODS: From January 2013 to December 2014, a survey was conducted in 9 sentinel STD clinics in Laoning province and other 6 provinces. Newly diagnosed STD patients were asked to fill out in STD reporting cards and encouraged to receive HIV test and counsel. Univariate and multivariate logistic regression analyses were conducted to identify the factors associated with the acceptance of HIV test. RESULTS: Of the 5063 STD cases reported, 2668(52.7%) received the HIV screening test, in which, 63(2.4%) were positive for HIV. Multivariate logistic regression analysis indicated that the acceptance of HIV test was associated with the location of STD clinic, patients' original living place, patient' s marital status, type of STD, and reasons for seeking medical care in STD clinic. CONCLUSION: HIV counsel and test were well accepted by STD patients, and the HIV positive rate was high. More studies are needed to further explore factors that impede the acceptance of HIV counsel and test services in STD clinics.


Assuntos
Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Instalações de Saúde/estatística & dados numéricos , China/epidemiologia , Aconselhamento/estatística & dados numéricos , Pesquisas sobre Serviços de Saúde , Humanos , Modelos Logísticos , Programas de Rastreamento/estatística & dados numéricos , Análise Multivariada , Aceitação pelo Paciente de Cuidados de Saúde/estatística & dados numéricos , Prevalência
8.
Biomed Chromatogr ; 30(4): 579-87, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26270156

RESUMO

Danggui-Shaoyao San (DSS) is a famous Chinese formula for activating blood circulation and promoting urination. This study was to investigate the difference of material basis between a blood-associated herbs group and a water-associated herbs group. According to the theory of traditional Chinese medicine, the formula can be divided into a blood-associated herbs group (Angelica sinensis, Paeonia lactiflora and Ligusticum chuanxiong) and a water-associated herbs group (Atractylodes macrocephala, Alisma orientale and Poria cocos). The HPLC fingerprint of the formula was established for quality control. Serum samples from rats, orally administrated DSS, and the decomposed recipes of DSS, were analyzed by HPLC-DAD and the transitional blood components of DSS were identified. Twenty-one common peaks were identified in the fingerprint of DSS. Contents of paeoniflorin, albiflorin, ferulic acid and alisol B 23-acetate in co-decoction were significantly higher than those in individual decoction. Eleven peaks belonged to the blood-associated herbs group (four metabolites and seven prototype components; paeoniflorin and ferulic acid appeared in prototype components), whereas six peaks belonged to the water-associated herbs group (three metabolites and three prototype components). It was concluded that the serum pharmacochemistry is a meaningful approach for clarifying the difference between blood-associated and water-associated herbs in chemical composition.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacocinética , Animais , Hidrocarbonetos Aromáticos com Pontes/análise , Colestenonas/análise , Cromatografia Líquida de Alta Pressão , Ácidos Cumáricos/análise , Medicamentos de Ervas Chinesas/administração & dosagem , Glucosídeos/análise , Masculino , Monoterpenos/análise , Ratos Sprague-Dawley , Soro/química , Solubilidade , Água/química
9.
Int J STD AIDS ; 26(6): 420-7, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25015933

RESUMO

Though vaginal douching is a common practice among female sex workers that could increase the risk of HIV and adverse reproductive health outcomes, it has drawn limited attention. From November 2010 to January 2011, a convenience sample of female sex workers was recruited in three cities in China. Face-to-face interviews were conducted to gather socio-demographic and behavioural information. Blood samples were collected for syphilis serological tests. Endo-cervical swabs were collected and tested for Neisseria gonorrhoeae and Chlamydia trachomatis by polymerase chain reaction. A logistic regression model was used to determine factors associated with vaginal douching and the association between vaginal douching and sexually transmitted infection. A total of 1032 eligible female sex workers were enrolled. The overall prevalence of any sexually transmitted infection (syphilis, Chlamydia trachomatis or Neisseria gonorrhoeae) and vaginal douching with disinfectant were 23.4% and 23.1%, respectively. Factors independently associated with douching practice included study sites, venue types, ethnicity, having regular partner and sexually transmitted infection history. No significant association was found between vaginal douching and current sexually transmitted infection. Vaginal douching with disinfectant after sex with clients seemed to be a prevalent practice among female sex workers in China. Prevention programmes targeting female sex workers should incorporate components about the adverse health outcomes associated with vaginal douching.


Assuntos
Trabalho Sexual , Profissionais do Sexo/estatística & dados numéricos , Comportamento Sexual , Doenças Sexualmente Transmissíveis/epidemiologia , Ducha Vaginal , Adulto , China/epidemiologia , Infecções por Chlamydia/epidemiologia , Chlamydia trachomatis/isolamento & purificação , Estudos Transversais , Feminino , Gonorreia/epidemiologia , Infecções por HIV/epidemiologia , Humanos , Entrevistas como Assunto , Modelos Logísticos , Pessoa de Meia-Idade , Neisseria gonorrhoeae/isolamento & purificação , Reação em Cadeia da Polimerase , Vigilância da População , Prevalência , Assunção de Riscos , Parceiros Sexuais , Doenças Sexualmente Transmissíveis/prevenção & controle , Fatores Socioeconômicos , Sífilis/epidemiologia
10.
Zhonghua Yu Fang Yi Xue Za Zhi ; 48(8): 693-8, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25388465

RESUMO

OBJECTIVE: To apply workbook method for the estimation on syphilis epidemic in China. METHODS: The data on population size and syphilis infection were collected by national STD and HIV/AIDS surveillance system among six populations aged from 15 to 49 years old in 31 provinces in 2011. Six groups included female sex workers (FSW), men who have sex with men (MSM), STD clinic attendee, drug users, pregnant women and general population. Meta analysis was applied to pool the different prevalence of the same population in the different sentinels of each province, and then workbook method was applied to estimate the syphilis epidemic in China. RESULTS: The estimation of syphilis epidemic(95%CI) was 2 979 422(1 504 000-6 063 309) among the populations aged 15 to 49 years old in 2011. The estimation among FSW was 54 624 (38 422-78 875) , that of MSM was 265 453 (162 586-506 520) , that of STD clinic attendee was 53 555 (31 256-98 057) , that of drug users was 94 244 (66 475-139 349) , that of pregnant women was 70 062 (39 942-136 584) and that of general population was 2 441 484 (1 165 319-5 103 924) . The proportion of general population in the whole estimation of syphilis infection was 81.94%, and that of MSM was 8.91%. The estimation of syphilis incidence was 1 489 711 among 15 to 49 years old, and the estimation of syphilis prevalence was 0.40% in the whole country. The estimation of incidence in this research was 5.2 times as the number of reported cases from China information system for diseases control and prevention. CONCLUSION: Workbook method was a scientific and feasible toolkit for the estimation of syphilis epidemic in China, and the estimation outcomes were greatly significant for syphilis control.


Assuntos
Métodos Epidemiológicos , Sífilis/epidemiologia , Adolescente , Adulto , China/epidemiologia , Usuários de Drogas , Epidemias/estatística & dados numéricos , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Gravidez , Prevalência , Comportamento Sexual
11.
Zhonghua Liu Xing Bing Xue Za Zhi ; 35(11): 1223-6, 2014 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-25598252

RESUMO

OBJECTIVE: To understand the prevalence rates of club drug use and how it associated with syphilis infection among female sex workers (FSWs) in different areas of China so as to provide reference for developing intervention programs. METHODS: In each of the study site, different kinds of venues where FSWs solicited clients were mapped and grouped into three strata. The number of FSWs at each venue was enumerated. A stratified proportional sampling design was adopted to recruit FSWs. Questionnaire survey was conducted to gather data on demographic information, club drug use and sexual behaviors. Blood samples were taken and tested for syphilis. Factors associated with syphilis infection and club drug use were assessed using univariate and multivariate logistic regression models. RESULTS: Results of the 1 630 participants, 120 (7.4%)used club drugs in the past 12 months, and 5.4% (88/1 630)tested positive for syphilis. Syphilis positive rate was not significantly different between FSWs who used club drugs and those who did not. Data from multivariate logistic regression analysis indicated that club drug use was associated with following factors as sites of the study, hometown of the FSWs, marital status, education level, having had STD symptoms or history of injecting drugs in the past 12 months of the sex workers. CONCLUSION: Club drug use was highly prevalent among FSWs. Further studies are warranted to fully explore the association between club drug use and the risk of HIV/STD transmission.


Assuntos
Drogas Ilícitas , Profissionais do Sexo/psicologia , Transtornos Relacionados ao Uso de Substâncias/epidemiologia , Sífilis/epidemiologia , China/epidemiologia , Feminino , Humanos , Prevalência , Profissionais do Sexo/estatística & dados numéricos , Comportamento Sexual
12.
Int J Radiat Oncol Biol Phys ; 74(4): 1242-50, 2009 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-19545790

RESUMO

PURPOSE: Oral mucositis is a common side effect induced by radio/chemotherapy in patients with head and neck cancer. Although it dramatically impairs patient quality of life, no efficient and safe therapeutic solution is available today. Therefore, we investigated the protective efficacy of a new heparan mimetic biopolymer, RGTA-OTR4131, used alone or in combination with amifostine, for oral mucositis and simultaneously evaluated its effect on tumor growth in vitro and in vivo. METHODS AND MATERIALS: A single dose of 16.5 Gy was selectively delivered to the snout of mice, and the effects of OTR4131 or amifostine-OTR4131 were analyzed by macroscopic scoring and histology. The effect of OTR4131 administration on tumor growth was then investigated in vitro and in xenograft models using two cell lines (HEP-2 and HT-29). RESULTS: Amifostine and OTR4131 significantly decreased the severity and duration of lip mucosal reactions. However, amifostine has to be administered before irradiation, whereas the most impressive protection was obtained when OTR4131 was injected 24 h after irradiation. In addition, OTR4131 was well tolerated, and the combination of amifostine and OTR4131 further enhanced mucosal protection. At the tumor level, OTR4131 did not modify HEP-2 cell line clonogenic survival in vitro or protect xenografted tumor cells from radiotherapy. Of interest, high doses of OTR4131 significantly decreased clonogenic survival of HT-29 cells. CONCLUSIONS: RGTAs-OTR4131 is a well-tolerated, natural agent that effectively reduces radio-induced mucositis without affecting tumor sensitivity to irradiation. This suggests a possible transfer into the clinic for patients' benefit.


Assuntos
Amifostina/uso terapêutico , Glucanos/uso terapêutico , Lábio/efeitos da radiação , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/uso terapêutico , Estomatite/prevenção & controle , Amifostina/administração & dosagem , Animais , Linhagem Celular Tumoral , Esquema de Medicação , Quimioterapia Combinada , Feminino , Glucanos/administração & dosagem , Células HT29 , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Nus , Doses de Radiação , Protetores contra Radiação/administração & dosagem , Distribuição Aleatória , Ensaios Antitumorais Modelo de Xenoenxerto/métodos
13.
Lancet ; 369(9556): 132-8, 2007 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-17223476

RESUMO

BACKGROUND: After a massive syphilis epidemic in the first half of the 20th century, China was able to eliminate this infection for 20 years (1960-80). However, substantial changes in Chinese society have been followed by a resurgent epidemic of sexually transmitted diseases. Sporadic reports have provided clues to the magnitude of the spread of syphilis, but a national surveillance effort is needed to provide data for planning and intervention. METHODS: We collected and assessed case report data from China's national sexually transmitted disease surveillance system and sentinel site network. FINDINGS: In 1993, the reported total rate of cases of syphilis in China was 0.2 cases per 100,000, whereas primary and secondary syphilis alone represented 5.7 cases per 100,000 persons in 2005. The rate of congenital syphilis increased greatly with an average yearly rise of 71.9%, from 0.01 cases per 100,000 livebirths in 1991 to 19.68 cases per 100 000 livebirths in 2005. INTERPRETATION: The results suggest that a range of unique biological and social forces are driving the spread of syphilis in China. A national campaign for detection and treatment of syphilis, and a credible prevention strategy, are urgently needed.


Assuntos
Vigilância de Evento Sentinela , Sífilis/epidemiologia , Adulto , China/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Sistema de Registros , Sífilis/classificação , Sífilis Congênita/epidemiologia
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