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1.
Eur Endod J ; 5(3): 219-225, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33353920

RESUMO

OBJECTIVE: The present clinical study investigated the microbiota of teeth with persistent secondary endodontic infection in the different phases of root canal retreatment. METHODS: Twenty filled single-rooted teeth with apical periodontitis were included. Samples were collected with sterile paper points before chemo-mechanical preparation (CMP) (S1), after CMP (S2) and after 30 days of calcium hydroxide-based intracanal medication (ICM) (S3). Cultivable bacteria were assessed by colony forming units count (CFU/mL). DNA was extracted and assessed by using nested PCR. Paired t-test and repeated measures ANOVA were applied for intragroup analysis in the stages of endodontic therapy at a significance level of 5%. RESULTS: Cultivable bacteria were detected in all initial samples. CMP reduced bacteria by 99.4% and ICM by 99.5%. The most prevalent species found in the initial samples were E. faecalis (20/20), P. gingivalis (20/20), F. nucleatum (17/20) and A. actinomycetemcomitans (10/20), whereas D. pneumosintes, F. alocis, P. nigrescens and T. socranskii were not detected. After CMP, A. israelii, A. naeslundii, G. morbillorum, T. forsythia and T. denticola were not detected (P<0.05) either. E. faecalis and P. gingivalis had a low reduction (P>0.05) and F. nucleatum had its DNA significantly reduced after CMP (P<0.05). ICM had no additional effect on microbial reduction. CONCLUSION: The microbiota of teeth with persistent/secondary endodontic infection consists of a polymicrobial community with Gram-positive and Gram-negative species, bacillus and cocci, facultative and strict anaerobes. E. faecalis and P. gingivalis were frequently detected in all stages of root canal retreatment, evidencing their great resistance to endodontic procedures. The endodontic procedures were effective in reducing the levels of bacteria from teeth presenting with persistent/secondary endodontic infection.

2.
Braz Dent J ; 31(5): 505-510, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33146334

RESUMO

The present study used microcomputed tomography (micro-CT) images to assess the prevalence, number, and location of apical ramifications (ARs) in the root canals of different dental groups in a Brazilian population. Three hundred and sixty-seven extracted human teeth, totaling 475 roots, were scanned by micro-CT at 19.6 µm spatial resolution. The obtained images were evaluated by two observers in consensus, and a descriptive analysis was performed to verify the prevalence, number, and location of ARs in each dental group. Chi-square test was used to compare the prevalence of ARs in different types of roots, and one-way analysis of variance compared the number of ARs in the apical 1 mm, 2 mm, and 3 mm of the root. Significance level was established at 5% (α=0.05). ARs were present in 253 (53.3%) of the 475 roots evaluated. A total of 607 ARs were detected, with the vast majority (530) located at one and two millimeters from the main foramen. The highest prevalence of ARs was observed in maxillary canines (65%); maxillary premolars - single-rooted (83.3%), buccal root (80%), and palatine root (73.3%); mandibular premolars (64.2%); mesiobuccal root of maxillary molars (76.6%); and mesial root of mandibular molars (66.6%). For the Brazilian population, most of the ARs are located in the apical 1 and 2 mm of the root, with greater prevalence in the posterior teeth and maxillary canines. These findings suggest the need for additional attention while planning endodontic treatments, including strategies to effectively clean and fill the apical region.


Assuntos
Cavidade Pulpar , Raiz Dentária , Brasil , Cavidade Pulpar/diagnóstico por imagem , Humanos , Dente Molar , Microtomografia por Raio-X
3.
Braz. dent. j ; 31(5): 505-510, Sept.-Oct. 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1132327

RESUMO

Abstract The present study used microcomputed tomography (micro-CT) images to assess the prevalence, number, and location of apical ramifications (ARs) in the root canals of different dental groups in a Brazilian population. Three hundred and sixty-seven extracted human teeth, totaling 475 roots, were scanned by micro-CT at 19.6 µm spatial resolution. The obtained images were evaluated by two observers in consensus, and a descriptive analysis was performed to verify the prevalence, number, and location of ARs in each dental group. Chi-square test was used to compare the prevalence of ARs in different types of roots, and one-way analysis of variance compared the number of ARs in the apical 1 mm, 2 mm, and 3 mm of the root. Significance level was established at 5% (α=0.05). ARs were present in 253 (53.3%) of the 475 roots evaluated. A total of 607 ARs were detected, with the vast majority (530) located at one and two millimeters from the main foramen. The highest prevalence of ARs was observed in maxillary canines (65%); maxillary premolars - single-rooted (83.3%), buccal root (80%), and palatine root (73.3%); mandibular premolars (64.2%); mesiobuccal root of maxillary molars (76.6%); and mesial root of mandibular molars (66.6%). For the Brazilian population, most of the ARs are located in the apical 1 and 2 mm of the root, with greater prevalence in the posterior teeth and maxillary canines. These findings suggest the need for additional attention while planning endodontic treatments, including strategies to effectively clean and fill the apical region.


Resumo O presente estudo utilizou imagens de microtomografia computadorizada (MicroCT) para avaliar a prevalência, número e localização de ramificações apicais (RAs) dos canais radiculares de diferentes grupos dentários em uma população brasileira. Trezentos e sessenta e sete dentes humanos extraídos (totalizando 475 raízes) foram escaneados em um aparelho de MicroCT, com uma resolução espacial de 19,6 µm. As imagens foram analisadas em consenso por dois avaliadores, e uma análise descritiva foi realizada para verificar a prevalência, número e localização de RAs em cada grupo dentário. O teste Qui-quadrado foi utilizado para comparar a prevalência de RAs em diferentes tipos de raízes e a análise de variância (ANOVA - um critério) comparou o número de RAs em 1 mm, 2 mm e 3 mm apicais da raiz. O nível de significância foi estabelecido em 5% (α=0,05). As RAs estavam presentes em 253 (53,3%) das 475 raízes avaliadas. Foram detectadas 607 RAs com a maioria (530) localizada a 1 e 2 milímetros do forame principal. A mais alta prevalência de RAs foi observada nos caninos superiores (65%); pré-molares superiores - unirradiculares (83,3%), raiz vestibular (80%) e raiz palatina (73,3%); pré-molares inferiores (64,2%); raiz mésio-vestibular dos molares superiores (76,6%); e raiz mesial dos molares inferiores (66,6%). Na população brasileira, a maioria dos RAs está localizada em 1 e 2 mm apicais da raiz, com maior prevalência nos dentes posteriores e nos caninos superiores. Esses achados sugerem atenção adicional ao planejar um tratamento endodôntico, bem como adoção de estratégias para uma limpeza e obturação efetiva da região apical dos dentes.

4.
J Appl Oral Sci ; 28: e20200269, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32785521

RESUMO

Tricalcium silicate-based cement are materials used in reparative and regenerative procedures in endodontics. A recently proposed formulation aimed to enhance handling during clinical use with a versatile material applicable by syringe. Although, the use of bismuth oxide as radiopacifier and grey raw powder are drawbacks considering aesthetics. Objectives Evaluate physicochemical, biological, and antimicrobial properties of Grey-MTAFlow (Ultradent) and assess whether the addition of zinc oxide (ZnO) prevents dentinal discoloration caused by bismuth oxide. Methodology Grey-MTAFlow was manipulated in 'thin' consistency for all tests. Luminosity, color change, ion migration to dentine, radiopacity, setting time, ISO 6876:2012 linear flow, volumetric lateral flow and central filling of simulated grooves scanned using micro-computed tomography (µCT), pH, calcium release, volumetric change using µCT, chemical characterisation, cytotoxicity, and antimicrobial activity were assessed. Addition of 5% ZnO to Grey-MTAFlow and a bismuth-containing experimental composition were comparatively tested. Statistical analyses used Shapiro-Wilk, T-test, ANOVA, and Kolmogorov-Smirnov (p<0.05). Results The addition of ZnO to Grey-MTAFlow prevented dentine darkening after 90 days due to bismuth migration reduction, although no statistical difference was found (p=0.863). ZnO addition significantly enhanced Grey-MTAFlow radiopacity without differences in initial setting time. Grey-MTAFlow presented an ISO linear flow of 10.9 mm and a balanced volumetric lateral flow with central filling in µCT evaluation. All compositions presented an alkaline pH after immersion. Grey-MTAFlow had a significantly higher calcium ion release after 28 days in comparison to 24 hours (p=0.011) and volumetric expansion of 0.4±1.8% after immersion. ZnO addition altered the hydrated cement matrix once calcium hydroxide (portlandite) could not be detected in characterisation. Neither of the materials produced inhibition halos nor reduced bacterial turbidity, but all presented cytocompatibility above 100%. Conclusion Grey-MTAFlow expanded after immersion and exhibited higher luminosity values after the evaluation period when ZnO was added, but chemical modifications after this addition occurred.


Assuntos
Anti-Infecciosos , Cimentos Dentários , Materiais Restauradores do Canal Radicular , Bismuto , Compostos de Cálcio , Combinação de Medicamentos , Teste de Materiais , Óxidos , Silicatos , Microtomografia por Raio-X
5.
Restor Dent Endod ; 45(3): e38, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32839719

RESUMO

Objectives: This study aimed to evaluate vital pulp tissue removal from different endodontic instrumentation systems from root canal apical third in vivo. Materials and Methods: Thirty mandibular molars were selected and randomly divided into 2 test groups and one control group. Inclusion criteria were a positive response to cold sensibility test, curvature angle between 10 and 20 degrees, and curvature radius lower than 10 mm. Root canals prepared with Hero 642 system (size 45/0.02) (n = 10) and Reciproc R40 (size 40/0.06) (n = 10) and control (n = 10) without instrumentation. Canals were irrigated only with saline solution during root canal preparation. The apical third was evaluated considering the touched/untouched perimeter and area to evaluate the efficacy of root canal wall debridement. Statistical analysis used t-test for comparisons. Results: Untouched root canal at cross-section perimeter, the Hero 642 system showed 41.44% ± 5.62% and Reciproc R40 58.67% ± 12.39% without contact with instruments. Regarding the untouched area, Hero 642 system showed 22.78% ± 6.42% and Reciproc R40 34.35% ± 8.52%. Neither instrument achieved complete cross-sectional root canal debridement. Hero 642 system rotary taper 0.02 instruments achieved significant greater wall contact perimeter and area compared to reciprocate the Reciproc R40 taper 0.06 instrument. Conclusions: Hero 642 achieved higher wall contact perimeter and area but, regardless of instrument size and taper, vital pulp during in vivo instrumentation is not entirely removed.

6.
J Endod ; 46(3): 404-412, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31937463

RESUMO

INTRODUCTION: The aim of this study was to analyze the contribution of bone marrow-derived cells (BMDCs) to reparative dentinogenesis using bone marrow transplantation (BMT) and pulp capping as an in vivo model. METHODS: A chimeric mouse model was created through the injection of BMDCs expressing green fluorescent protein (GFP+ BMDCs) from C57BL/6 GFP+ transgenic donor mice into irradiated C57BL/6 wild-type recipient mice (GFP- mice). These GFP- chimeric mice (containing transplanted GFP+ BMDCs) were subjected to microscopic pulp exposure and capping with white mineral trioxide aggregate (n = 18) or Biodentine (Septodont, St Maur-des-Fossés, France) (n = 18) in the maxillary first molar. Maxillary arches from GFP- chimeric mice (with the capped tooth) were isolated and histologically processed 5 (n = 9) and 7 (n = 9) weeks after BMT. Confocal laser microscopy and immunohistochemical analysis were performed to assess the presence of GFP+ BMDCs and the expression of dentin sialoprotein, an odontoblast marker, for those cells contributing to reparative dentinogenesis in the dental pulp. RESULTS: Confocal laser microscopic analyses evidenced the presence of GFP+ BMDCs in close association with reparative dentin synthesized at the site of pulp exposure in GFP- mice 5 and 7 weeks after BMT. Immunohistochemical analysis revealed that GFP+ BMDCs in close association with reparative dentin expressed DSP, suggesting the contribution of nonresident GFP+ BMDCs to reparative dentinogenesis. CONCLUSIONS: These data suggest the presence of nonresident BMDCs in reparative dentinogenesis and its contribution to dental pulp regeneration in the pulp healing process.


Assuntos
Transplante de Medula Óssea , Dentinogênese , Animais , Medula Óssea , Células da Medula Óssea , Polpa Dentária , França , Camundongos , Camundongos Endogâmicos C57BL
7.
Rev. bras. odontol ; 77(1): 1-7, jan. 2020. ilus; tab
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1102870

RESUMO

Objective: to evaluate the root canal treatment (RCT) and non-surgical root canal retreatment (NSRCR), associated with foraminal enlargement, performed on a singlevisit. Material and Methods: 125 teeth with apical periodontitis and follow-up period ranging from 6 to 12 months were included. The success was considered by the absence of signs and symptoms and complete or incomplete periapical repair. Logistic regression analyses were used to identify factors associated with the repair (p<0.05). Results: RCT showed 71.58% of complete healing and 23.16% of acceptable healing. NSRCR showed 80% of complete healing and 20% of acceptable healing. Age, gender, type of treatment and preoperative pain were not statistically significant for the healing process (p>0.05). Premolars showed the greatest chance of periapical repair. Pulp Canal Sealer showed a greater chance of periapical repair when compared to Sealapex (p<0.05). Conclusion: RCT and NSRCR using a foraminal enlargement protocol provided a favorable prognosis of periapical healing


Objetivo: avaliar o tratamento endodôntico (TE) e o retratamento não cirúrgico do canal radicular (RNCCR), associado a ampliação foraminal, realizado em sessão única. Material e Métodos: foram incluídos 125 dentes com periodontite apical e proservação de 6 a 12 meses. O sucesso foi considerado pela ausência de sinais e sintomas e reparo periapical completo ou incompleto. Análises de regressão logística foram utilizadas para identificar fatores associados ao reparo (p<0,05). Resultados: o TE mostrou 71,58% de cicatrização completa e 23,16% de cicatrização aceitável. O RNCCR mostrou 80% e 20% cicatrização completa e aceitável, respectivamente. Idade, sexo, tipo de tratamento e dor pré-operatória não foram estatisticamente significantes para o processo de cicatrização (p>0,05). Os pré-molares apresentaram a maior chance de reparo periapical. O cimento Pulp Canal Sealer mostrou maior chance de reparo periapical quando comparado ao Sealapex (p<0,05). Conclusão: o TE e RNCCR utilizando um protocolo de alargamento foraminal, proporcionaram um prognóstico favorável da cicatrização periapical


Assuntos
Periodontite Periapical , Tratamento do Canal Radicular/métodos , Preparo de Canal Radicular
8.
J. appl. oral sci ; 28: e20200269, 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1134788

RESUMO

Abstract Tricalcium silicate-based cement are materials used in reparative and regenerative procedures in endodontics. A recently proposed formulation aimed to enhance handling during clinical use with a versatile material applicable by syringe. Although, the use of bismuth oxide as radiopacifier and grey raw powder are drawbacks considering aesthetics. Objectives Evaluate physicochemical, biological, and antimicrobial properties of Grey-MTAFlow (Ultradent) and assess whether the addition of zinc oxide (ZnO) prevents dentinal discoloration caused by bismuth oxide. Methodology Grey-MTAFlow was manipulated in 'thin' consistency for all tests. Luminosity, color change, ion migration to dentine, radiopacity, setting time, ISO 6876:2012 linear flow, volumetric lateral flow and central filling of simulated grooves scanned using micro-computed tomography (μCT), pH, calcium release, volumetric change using μCT, chemical characterisation, cytotoxicity, and antimicrobial activity were assessed. Addition of 5% ZnO to Grey-MTAFlow and a bismuth-containing experimental composition were comparatively tested. Statistical analyses used Shapiro-Wilk, T-test, ANOVA, and Kolmogorov-Smirnov (p<0.05). Results The addition of ZnO to Grey-MTAFlow prevented dentine darkening after 90 days due to bismuth migration reduction, although no statistical difference was found (p=0.863). ZnO addition significantly enhanced Grey-MTAFlow radiopacity without differences in initial setting time. Grey-MTAFlow presented an ISO linear flow of 10.9 mm and a balanced volumetric lateral flow with central filling in μCT evaluation. All compositions presented an alkaline pH after immersion. Grey-MTAFlow had a significantly higher calcium ion release after 28 days in comparison to 24 hours (p=0.011) and volumetric expansion of 0.4±1.8% after immersion. ZnO addition altered the hydrated cement matrix once calcium hydroxide (portlandite) could not be detected in characterisation. Neither of the materials produced inhibition halos nor reduced bacterial turbidity, but all presented cytocompatibility above 100%. Conclusion Grey-MTAFlow expanded after immersion and exhibited higher luminosity values after the evaluation period when ZnO was added, but chemical modifications after this addition occurred.

9.
Dent. press endod ; 9(1): 72-81, jan.-mar. 2019.
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1024254

RESUMO

Introdução: um dos objetivos da terapia endodôntica é a redução dos fatores microbianos no interior dos canais radiculares, o que pode ser conseguido por meio do preparo químico-mecânico (PQM). O PQM tem como finalidade criar um ambiente propício à condição de reparo, por meio da limpeza e modelagem do sistema de canais radiculares (SCR), que associa a ação mecânica dos instrumentos ao uso de substâncias químicas auxiliares. Em relação ao limite apical desse preparo e ao diâmetro apical final da instrumentação, podemos observar que não há consenso entre os profissionais. Introdução: o objetivo do presente estudo foi avaliar, por meio de uma revisão de literatura, a eficiência de duas técnicas no preparo e desinfecção do terço apical do SCR: a técnica de ampliação foraminal e a técnica de batente apical com manutenção da patência foraminal. Métodos: foi realizada uma busca nas bases de dados PubMed, Medline, BBO, Lilacs e SciELO, usando os seguintes termos: canal radicular, forame apical, endodontia, lima de patência, tamanho apical, lima apical final, preparo biomecânico, ampliação foraminal, comprimento de trabalho, anatomia dental, tratamento endodôntico, patência foraminal, preparo do canal radicular e batente apical. Cada um desses termos foi combinado utilizando os operadores booleanos E/OU. Após leitura dos resumos, 65 artigos foram selecionados e incluídos nessa revisão. Resultados: os resultados demonstraram que o alargamento do terço apical, seja com ou sem ampliação foraminal, parece favorecer o prognóstico do tratamento endodôntico, devido à redução do conteúdo infeccioso em toda a extensão do SCR. A manutenção da patência foraminal é um fator essencial para um PQM adequado quando a técnica de batente apical é realizada. Conclusão: conclui-se que preparos apicais mais amplos promovem resultados mais previsíveis, e que a ampliação foraminal possibilita o melhor debridamento e desinfeção do SCR (AU).


Introduction: Endodontic therapy should reduce the microbial load within the root canals, which can be achieved during chemomechanical preparation (CMP). The purpose of CMP of the root canal system (RCS) is to create a favorable environment for healing. CMP associates the mechanical action of instruments with the use of auxiliary chemical substances. There is no consensus among professionals about what should be the diameter of the apical foramen after CMP. This literature review evaluated the efficiency of two apical preparation techniques: foraminal enlargement and apical stop with maintenance of foraminal patency. Material and methods: A literature search was conducted in PubMed, Medline, BBO, Lilacs and Scielo using the following terms: root canal, apical foramen, endodontics, patency file, apical size, master apical file, biomechanical preparation, foraminal enlargement, working length, dental anatomy, endodontic treatment, foraminal patency, root canal preparation, and apical stop. All terms were combined using the Boolean operators AND/OR. After the abstracts were analyzed, 65 full texts were selected and included in this review. Results: The enlargement of the apical third, with or without foraminal enlargement, seems to improve endodontic treatment prognosis due to the reduction of infectious contents throughout the RCS. Foraminal patency is essential for adequateCMP when the apical stop technique is performed. Conclusion: Apical preparations with a greater diameter have more predictable results, and foraminal enlargement ensures better debridement and disinfection of the RCS (AU).


Assuntos
Tratamento do Canal Radicular , Preparo de Canal Radicular , Doenças Periapicais , Ápice Dentário
10.
Clin Oral Investig ; 23(6): 2759-2766, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30367257

RESUMO

OBJECTIVE: The objective of this work was to investigate in vivo the effects of calcium hydroxide-based intracanal medication (ICM) on the levels of bacteria, pro-inflammatory cytokines (PICs), and matrix metalloproteinases (MMPs) in root canals and periradicular tissues of teeth with failure of the root canal treatment and apical periodontitis. MATERIALS AND METHODS: Twenty infected root canals of single-rooted teeth were randomly assigned into two groups according to the irrigant used for chemomechanical preparation (CMP) (n = 10 per group): G1 - 2% chlorhexidine (CHX) gel and G2 - 6% sodium hypochlorite (NaOCl). Root canal contents were taken by using paper points before CMP (S1) and after 30 days of calcium hydroxide-based ICM (S2). Microbial reduction was calculated by means of colony-forming unit count (CFU/mL), with PICs and MMPs (pg/mL) being measured by using enzyme-linked immunosorbent assay (ELISA). RESULTS: Culturable bacteria (101.2 ± 79.2), PICs (IL-1ß 1.2 ± 0.4 and TNF-α 8.8 ± 4.7), MMP-2 (803.7 ± 96.4), MMP-3 (453.9 ± 229.3), MMP-8 (245.9 ± 122.4), MMP-9 (129.4 ± 29.6), and MMP-13 (70.8 ± 12.8) were present in all S1 samples. After 30 days of ICM (S2), a 99.5% microbial reduction was observed, together with a significant reduction of PICs in all groups. Overall, it was observed a decrease in the levels of MMPs (S2), except MMP-13, which was found in increased levels after ICM (P < .05), independently of the groups. CONCLUSIONS: Calcium hydroxide-based intracanal medications have had a positive effect on the microbial reduction by decreasing the levels of PICs and MMPs. Both auxiliary chemical substances (i.e., 2% CHX and 6% NaOCl) presented similar effects when calcium hydroxide was used as intracanal medication. CLINICAL RELEVANCE: Teeth with failure of the root canal treatment and apical periodontitis, and consequently with high levels of bacteria, PIC, and MMP, may present a better prognosis after a 30 days of a calcium hydroxide-based ICM.


Assuntos
Hidróxido de Cálcio/uso terapêutico , Clorexidina/uso terapêutico , Periodontite Periapical/complicações , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular , Hipoclorito de Sódio/uso terapêutico , Adulto , Bactérias/isolamento & purificação , Contagem de Colônia Microbiana , Citocinas/metabolismo , Cavidade Pulpar/microbiologia , Humanos , Metaloproteinases da Matriz/metabolismo , Pessoa de Meia-Idade , Periodontite Periapical/microbiologia
11.
RGO (Porto Alegre) ; 66(4): 345-351, Oct.-Dec. 2018. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-984917

RESUMO

ABSTRACT Objective: This retrospective study evaluated the demographic profile of patients and the clinical characteristics of dental emergencies in a brazilian university outpatient clinic. Methods: During the period from June 2013 to May 2014, data were collected from 1.345 clinical records. Chief complaint, gender, age, systemic alteration, clinical inspection of the affected tooth, pulp and periapical diagnosis, the type of procedure performed and whether there was the need for post-operative systemic medication. Data were analyzed using the SAS software and chi-square test was used to verify the presence or absence of statistical significance at a level of 5%. Results: Dental pain (53.3%) was the main reason why people attended the emergency service, that is, female patients (63.1%), aged 29-48 years old (42%), patients without systemic conditions (75%), and patients with decayed teeth (25%). In addition, involvement of lower molars (29.81%) and diagnosis of irreversible pulpitis (30.42%) were the most frequent findings. Emergency endodontic treatment (45.8%) was the most performed procedure. Irreversible pulpitis (30.4%) and pulp necrosis associated with acute apical periodontitis (21.9%) were the most frequent conditions requiring emergency endodontic treatment, and in most cases, there was no need for post-operative systemic medications (95.7%). Conclusion: The prevalence of endodontic emergencies during the period of one year emphasizes the need for more accurate diagnosis and the importance of providing this service to the population.


RESUMO Objetivo: Este estudo retrospectivo avaliou o perfil demográfico dos pacientes e as características clínicas das urgências dentárias em um Serviço de Plantão de Urgências de uma Faculdade brasileira de Odontologia. Métodos: Durante o período de junho de 2013 a maio de 2014, os dados foram coletados de 1.345 registros clínicos, com informações sobre a queixa principal, gênero, idade, alteração sistêmica, inspeção clínica do dente afetado, diagnóstico pulpar e periapical, o tipo de procedimento realizado e se houve necessidade de medicação sistêmica pós-operatória. Os dados foram analisados usando o software SAS e o teste do qui-quadrado foi utilizado para verificar a presença ou ausência de significância estatística ao nível de 5%. Resultados: A dor dental (53,3%) foi a queixa principal mais encontrada. Pacientes do gênero feminino (63,1%), com idade entre 29-48 anos (42%), sem condições sistêmicas (75%) e com dentes cariados (25%) foram os mais prevalentes. Além disso, o envolvimento de molares inferiores (29,81%) e o diagnóstico de pulpite irreversível (30,42%) foram os achados mais frequentes. O tratamento endodôntico de urgência (45,8%) foi o procedimento mais realizado. A pulpite irreversível (30,4%) e a necrose pulpar associada a periodontite apical aguda (21,9%) foram as condições mais frequentes que necessitaram de tratamento endodôntico de urgência e, na maioria dos casos, não houve prescrição de medicamentos sistêmicos pós-operatórios (95,7%). Conclusão: A prevalência de urgências endodônticas durante o período de um ano enfatiza a necessidade de diagnósticos mais precisos e a importância de oferecer esse serviço à população.

12.
Braz Oral Res ; 32: e33, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29723333

RESUMO

This study aimed to evaluate the influence of different ethanol concentrations on dentin roughness, surface free energy, and contact angle between AH Plus and the root canal dentin. One hundred human maxillary anterior teeth were split longitudinally and 200 dentin specimens were polished to make the surface flatter and smoother. An acrylic bar was positioned between two dentin specimens and impression material was added to create a block, simulating an instrumented root canal space. Specimens were removed from the mold and cleaned in an ultrasonic bath for 10 min. Thereafter, dentin specimens were divided into four groups (n = 50) according to the drying methods used: a) wet: vacuum only, b) paper points: vacuum + absorbent paper points, c) 70% alcohol: 70% alcohol (1 min) + vacuum + absorbent paper points, and d) 100% alcohol: 100% alcohol (1 min) + vacuum + absorbent paper points. A rugosimeter and a goniometer were used to verify the roughness (Ra) and to measure the surface free energy and the contact angle between the AH Plus sealer and the root canal dentin. ANOVA and Tukey tests (α = 0.05) were used for statistical analysis. The 70% and 100% ethanol groups showed significantly decreased roughness as well as increased surface free energy in the root canal dentin when compared to the wet and paper point groups. In addition, ethanol significantly reduced the contact angle between the AH Plus sealer and the root canal dentin. Ethanol solutions (70% and 100%) provide better wettability of AH Plus sealer on dentin surfaces.


Assuntos
Dentina/química , Dentina/efeitos dos fármacos , Resinas Epóxi/química , Etanol/química , Materiais Restauradores do Canal Radicular/química , Raiz Dentária/química , Raiz Dentária/efeitos dos fármacos , Análise de Variância , Colagem Dentária/métodos , Dentina/ultraestrutura , Humanos , Teste de Materiais , Reprodutibilidade dos Testes , Propriedades de Superfície/efeitos dos fármacos , Molhabilidade
13.
J Endod ; 44(3): 438-445, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29275852

RESUMO

INTRODUCTION: This study aimed to evaluate the time required for bone marrow-derived cells (BMDCs) from transgenic green fluorescent protein (GFP)+ donor mice (GFP+ mice) to migrate into the dental pulp of wild-type GFP- recipient mice (GFP- mice) by using bone marrow transplantation (BMT) as an in vivo model for tracking BMDCs from GFP+ mice (GFP+ BMDCs). METHODS: GFP+ BMDCs were injected into irradiated GFP- mice. Maxillary arches, tibiae, and femora from GFP- mice were isolated and processed at 24 hours, 48 hours, 4, 7, and 14 days, and 7 weeks after BMT. Confocal laser microscopy analyses were performed to assess the presence of GFP+ BMDCs in the dental pulp, and flow cytometry of BM was performed to confirm the efficiency of engraftment of GFP+ BMDCs. RESULTS: Confocal laser microscopy analyses evidenced the presence of GFP+ BMDCs in the dental pulp of GFP- mice from 14 days to 7 weeks after BMT. There was no presence of GFP+ BMDCs at 24 hours, 48 hours, 4 days, and 7 days. Flow cytometry of the BM of GFP- mice demonstrated a constant increase in the presence of GFP+ BMDCs at 24 hours, 48 hours, and 4 days after BMT, which stabilized from 7 days to 7 weeks. CONCLUSIONS: The study demonstrated the presence of GFP+ BMDCs in the dental pulp from 14 days to 7 weeks after BMT and the feasibility of using GFP+ animals and BMT as an in vivo model for tracking GFP+ BMDCs.


Assuntos
Células da Medula Óssea/fisiologia , Transplante de Medula Óssea , Movimento Celular , Polpa Dentária , Animais , Feminino , Proteínas de Fluorescência Verde/genética , Camundongos , Camundongos Transgênicos , Fatores de Tempo
14.
Braz. oral res. (Online) ; 32: e33, 2018. graf
Artigo em Inglês | LILACS | ID: biblio-889462

RESUMO

Abstract This study aimed to evaluate the influence of different ethanol concentrations on dentin roughness, surface free energy, and contact angle between AH Plus and the root canal dentin. One hundred human maxillary anterior teeth were split longitudinally and 200 dentin specimens were polished to make the surface flatter and smoother. An acrylic bar was positioned between two dentin specimens and impression material was added to create a block, simulating an instrumented root canal space. Specimens were removed from the mold and cleaned in an ultrasonic bath for 10 min. Thereafter, dentin specimens were divided into four groups (n = 50) according to the drying methods used: a) wet: vacuum only, b) paper points: vacuum + absorbent paper points, c) 70% alcohol: 70% alcohol (1 min) + vacuum + absorbent paper points, and d) 100% alcohol: 100% alcohol (1 min) + vacuum + absorbent paper points. A rugosimeter and a goniometer were used to verify the roughness (Ra) and to measure the surface free energy and the contact angle between the AH Plus sealer and the root canal dentin. ANOVA and Tukey tests (α = 0.05) were used for statistical analysis. The 70% and 100% ethanol groups showed significantly decreased roughness as well as increased surface free energy in the root canal dentin when compared to the wet and paper point groups. In addition, ethanol significantly reduced the contact angle between the AH Plus sealer and the root canal dentin. Ethanol solutions (70% and 100%) provide better wettability of AH Plus sealer on dentin surfaces.


Assuntos
Humanos , Dentina/química , Dentina/efeitos dos fármacos , Resinas Epóxi/química , Etanol/química , Materiais Restauradores do Canal Radicular/química , Raiz Dentária/química , Raiz Dentária/efeitos dos fármacos , Análise de Variância , Colagem Dentária/métodos , Dentina/ultraestrutura , Teste de Materiais , Reprodutibilidade dos Testes , Propriedades de Superfície/efeitos dos fármacos , Molhabilidade
15.
Restor Dent Endod ; 42(2): 140-145, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28503480

RESUMO

OBJECTIVES: The aim of this randomized, controlled, prospective clinical study was to evaluate patients' intraoperative discomfort during root canal preparations in which either multi-file rotary (Mtwo) or single-file reciprocating (Reciproc) systems were used. MATERIALS AND METHODS: Fifty-five adult patients, aged between 25 and 69 years old, with irreversible pulpitis or pulp necrosis participated in this study. Either the mesiobuccal or the distobuccal canals for maxillary molars and either the mesiobuccal or the mesiolingual canals for mandibular molars were randomly chosen to be instrumented with Mtwo multi-file rotary or Reciproc single-file reciprocating systems. Immediately after each canal instrumentation under anesthesia, patient discomfort was assessed using a 1 - 10 visual analog scale (VAS), ranging from 'least possible discomfort' (1) to 'greatest possible discomfort' (10). The Wilcoxon signed-rank test was used to determine significant differences at p< 0.05. RESULTS: Little intraoperative discomfort was found in all cases. No statistically significant differences in intraoperative discomfort between the 2 systems were found (p = 0.660). CONCLUSIONS: Root canal preparation with multi-file rotary or single-file reciprocating systems had similar and minimal effects on patients' intraoperative discomfort.

16.
Quintessence Int ; 48(7): 555-561, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28555199

RESUMO

Intrusive luxation is a severe trauma-related event with a high frequency of pulp necrosis and root resorption. A common complication following intrusion is tooth ankylosis, which can extend to progressive replacement root resorption. An increasing infraposition in growing individuals can also occur. Decoronation is a new conservative treatment for cases of tooth ankylosis in young patients who have not finished growing. In this surgical technique, the tooth crown is removed and the root with replacement resorption process remains inside the alveolus. This treatment allows the alveolar bone to continue to develop, thus preserving bone dimensions. The purpose of this article is to report a multidisciplinary case of a young patient with anterior open bite, dental ankylosis, and tooth infraposition affected by intrusive luxation. Decoronation was the treatment of choice for this patient. The patient was then referred to orthodontic treatment to maintain the space of the maxillary right central incisor and open bite correction. After 5 years of follow-up, good clinical and radiologic results were obtained.


Assuntos
Ortodontia Corretiva/métodos , Reabsorção da Raiz/terapia , Mantenedor de Espaço em Ortodontia/métodos , Anquilose Dental/terapia , Coroa do Dente/cirurgia , Acidentes por Quedas , Criança , Terapia Combinada , Humanos , Incisivo/lesões , Masculino , Mordida Aberta/terapia , Reabsorção da Raiz/etiologia , Anquilose Dental/etiologia
17.
Braz Dent J ; 28(2): 201-205, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28492750

RESUMO

This clinical study aimed to evaluate the relationship of the delay between dental trauma and the initial attendance to the development of external inflammatory root resorption in permanent teeth affected by severe luxation. Sixty-seven patients, aged between 11 and 56 years, presenting 133 injured teeth with closed apex (56 extrusive luxation, 69 lateral luxation and 8 intrusive luxation) were followed up for a minimum of 24 months. The time elapsed between dental trauma and the initial attendance was recorded. The presence of necrotic pulp and external inflammatory resorption for each type of trauma was verified. Fisher's exact test was used to verify the influence of the initial attendance date at the Dental Trauma Center and the development of inflammatory resorption (p<0.05). The odds ratio was used to check the risk of developing external inflammatory resorption due to delay in seeking treatment. Pulp necrosis was observed in 105 teeth (78.9%) and external inflammatory resorption was detected in 17.8% cases of extrusive luxation (10 teeth), 15.9% of lateral luxation (11 teeth) and 25% of intrusive luxation (2 teeth). For lateral luxation, extended delay between the date of injury and initial attendance influenced the occurrence of external inflammatory resorption (p=0.0174). Patients who began treatment 45 days after the injury were 3.4 times more likely to develop external inflammatory resorption than patients who sought treatment after the trauma. Treatment late after the occurrence of dental trauma can impair the prognosis and result in the development of external inflammatory resorption in luxated teeth.


Assuntos
Inflamação/fisiopatologia , Reabsorção da Raiz , Avulsão Dentária/fisiopatologia , Criança , Feminino , História do Século XVII , Humanos , Masculino , Adulto Jovem
18.
Braz. dent. j ; 28(2): 201-205, mar.-Apr. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-839131

RESUMO

Abstract This clinical study aimed to evaluate the relationship of the delay between dental trauma and the initial attendance to the development of external inflammatory root resorption in permanent teeth affected by severe luxation. Sixty-seven patients, aged between 11 and 56 years, presenting 133 injured teeth with closed apex (56 extrusive luxation, 69 lateral luxation and 8 intrusive luxation) were followed up for a minimum of 24 months. The time elapsed between dental trauma and the initial attendance was recorded. The presence of necrotic pulp and external inflammatory resorption for each type of trauma was verified. Fisher’s exact test was used to verify the influence of the initial attendance date at the Dental Trauma Center and the development of inflammatory resorption (p<0.05). The odds ratio was used to check the risk of developing external inflammatory resorption due to delay in seeking treatment. Pulp necrosis was observed in 105 teeth (78.9%) and external inflammatory resorption was detected in 17.8% cases of extrusive luxation (10 teeth), 15.9% of lateral luxation (11 teeth) and 25% of intrusive luxation (2 teeth). For lateral luxation, extended delay between the date of injury and initial attendance influenced the occurrence of external inflammatory resorption (p=0.0174). Patients who began treatment 45 days after the injury were 3.4 times more likely to develop external inflammatory resorption than patients who sought treatment after the trauma. Treatment late after the occurrence of dental trauma can impair the prognosis and result in the development of external inflammatory resorption in luxated teeth.


Resumo O objetivo deste estudo clínico foi avaliar a relação entre a demora na procura por tratamento e o desenvolvimento de reabsorções inflamatórias externas em dentes luxados com ápice fechado. A amostra desta pesquisa foi constituída por 67 pacientes que apresentaram 133 dentes traumatizados, dos quais 56 foram acometidos por luxação extrusiva, 69 por luxação lateral e 8 por luxação intrusiva, proservados por no mínimo 24 meses. Foi registrado o tempo decorrido entre a data do trauma e o primeiro atendimento. Verificou-se a ocorrência de necrose pulpar e reabsorções inflamatórias externas em cada tipo de traumatismo. O Teste Exato de Fisher foi aplicado para verificar a influência da data do atendimento inicial e o desenvolvimento de reabsorções inflamatórias externas em cada tipo de luxação e o teste de Odds Ratio foi aplicado para verificar o risco do desenvolvimento de reabsorções inflamatórias externas em função da demora na procura por tratamento. Os resultados revelaram que a necrose pulpar foi verificada em 105 dentes luxados (78,9%) e a reabsorção inflamatória externa foi verificada em 17.8% das luxações extrusivas (10), sendo 15,9% das luxações laterais (11) e 25% das luxações intrusivas (2). A demora na procura por tratamento influenciou o desenvolvimento de reabsorções infamatórias externas nas luxações laterais (p=0,0174). Os pacientes que procuraram tratamento após 45 dias da data do trauma apresentaram 3,4 vezes mais chance de desenvolver reabsorção inflamatória externa do que os pacientes que procuraram tratamento logo após o traumatismo. Conclui-se que o tratamento tardio após a ocorrência do traumatismo pode prejudicar o prognóstico e favorecer o desenvolvimento de reabsorções inflamatórias externas em dentes luxados com ápice fechado.


Assuntos
Humanos , Masculino , Feminino , Criança , História do Século XVII , Adulto Jovem , Inflamação/fisiopatologia , Reabsorção da Raiz , Avulsão Dentária/fisiopatologia
19.
J Endod ; 43(4): 619-622, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28216274

RESUMO

INTRODUCTION: The aim of the present study was to evaluate the percentage frequency of dentinal micro-cracks observed after root canal preparation with TRUShape and Self-Adjusting File (SAF) systems by means of micro-computed tomography imaging analysis. A conventional full-sequence rotary system (BioRace) and a single-file reciprocation system (Reciproc) were used as reference techniques for comparison because of their known assertive cutting efficiency. METHODS: Forty anatomically matched mandibular incisors were selected, scanned at a resolution of 14.25 µm, and assigned to 4 experimental groups (n = 10), according to the preparation protocol: TRUShape, SAF, BioRace, and Reciproc systems. After the experimental procedures, the specimens were scanned again, and the registered preoperative and postoperative cross-section images of the roots (n = 70,030) were screened to identify the presence of dentinal micro-cracks. RESULTS: Overall, dentinal defects were observed in 28,790 cross-section images (41.11%). In the TRUShape, SAF, BioRace, and Reciproc groups, dentinal micro-cracks were visualized in 56.47% (n = 9842), 42.38% (n = 7450), 32.90% (n = 5826), and 32.77% (n = 5672) of the slices, respectively. All dentinal defects observed in the postoperative data sets were already present in the corresponding preoperative images. CONCLUSIONS: None of the preparation systems induced the formation of new dentinal micro-cracks.


Assuntos
Radiografia Dentária , Preparo de Canal Radicular/efeitos adversos , Fraturas dos Dentes/etiologia , Microtomografia por Raio-X , Dentina/lesões , Humanos , Incisivo/lesões , Incisivo/cirurgia , Radiografia Dentária/métodos , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Fraturas dos Dentes/dietoterapia , Microtomografia por Raio-X/métodos
20.
Braz Dent J ; 27(6): 652-656, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27982174

RESUMO

The aim of the present study was to evaluate the cytotoxic effects of five endodontic sealers (AH Plus, Endomethasone N, EndoSequence BC, MTA Fillapex and Pulp Canal Sealer EWT) using a three-dimensional (3D) cell culture model. A conventional bi-dimensional (2D) cell culture model was used as reference technique for comparison. Balb/c 3T3 fibroblasts were cultured in conventional bi-dimensional cell culture and in rat-tail collagen type I three-dimensional cell culture models. Then, both cell cultures were incubated with elutes of freshly mixed endodontic sealers for 24 h. Cell viability was measured by the methyl-thiazol-diphenyltetrazolium assay (MTT). Data were statistically analyzed using ANOVA and the Tukey test at a significance level of p<0.05. All tested sealers exhibited cytotoxic effects; however, cytotoxic effect was culture model- and sealer-dependent. Sealers showed higher cytotoxicity in 2D than in 3D cell culture model (p<0.05). In both conditions, EndoSequence BC showed the lowest cytotoxicity (p<0.05). MTA Fillapex was much more cytotoxic than the other tested endodontic sealers (p<0.05), with the exception of AH Plus in the 2D cell culture model (p>0.05). Endomethasone N and Pulp Canal Sealer EWT showed lower cytotoxic effects than AH Plus in 2D cell culture model (p<0.05); however no statistical differences was observed among these sealers in 3D cell culture model. It may be concluded that cytotoxicity was higher in 2D cell culture compared to 3D cell culture. EndoSequence BC sealer exhibited the highest cytocompatibility and MTA Fillapex the lowest cytocompatibility.


Assuntos
Materiais Restauradores do Canal Radicular , Células 3T3 , Animais , Camundongos , Camundongos Endogâmicos BALB C
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