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1.
Vestn Oftalmol ; 135(5. Vyp. 2): 192-198, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31691659

RESUMO

Ophthalmologic manifestation of Sjogren's disease (SD) and rheumatoid arthritis (RA) is dry keratoconjunctivitis (dry eye disease; DED). PURPOSE: To study the relationship of polymorphic markers rs7947461 (C/T), rs915956 (C/T), rs4144331 (C/A) of the TRIM21 gene with the severity of DED in patients with RA and SD. MATERIAL AND METHODS: The study included 70 patients with RA (n=27) and SD (n=43). The control group consisted of volunteers without a history of RA or SD (n=35). Alleles of the polymorphic marker C660T rs7947461 of the TRIM21 gene were identified using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method; alleles of the polymorphic marker rs915956 (C/T) and rs4144331 (C/A) of the TRIM21 gene were identified by analyzing DNA melting curves. RESULTS: An association was found between the predisposing genotype (TT) of rs7947461 polymorphic marker and the risk of developing severe DED. The AA genotype of rs4144331 polymorphic marker was found only in severe DED (c2=7.74; OR=17.46, CI95%=1.96-318.38, p=0.02). CONCLUSION: An association was established between rs7947461 (rs660) and rs4144331 and the risk of developing severe DED.


Assuntos
Artrite Reumatoide , Ceratoconjuntivite , Ribonucleoproteínas/genética , Síndrome de Sjogren , Alelos , Artrite Reumatoide/genética , Predisposição Genética para Doença , Genótipo , Humanos , Ceratoconjuntivite/genética , Polimorfismo Genético , Síndrome de Sjogren/genética
2.
Vestn Oftalmol ; 135(5. Vyp. 2): 254-259, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31691669

RESUMO

The article reviews literature on relationships between polymorphic variants of the genes THBS1, GTF2I, MUC1, TRIM21, STAT4, PTPN22 with clinical features of dry keratoconjunctivitis in rheumatoid arthritis and Sjogren's syndrome. The development and implementation of a method for analyzing polymorphic gene variants used to diagnose dry keratoconjunctivitis in rheumatoid arthritis and Sjogren's syndrome will allow assessment of the possibility of developing dry keratoconjunctivitis and/or its progression in patients with autoimmune diseases or in people at risk. Determination of clinical and morphological regularities of dry keratoconjunctivitis in accordance with the revealed molecular and genetic changes will contribute to better understanding of the etiology and pathogenesis of ophthalmological manifestations of autoimmune diseases, and will also help improve the diagnostics and prognosis of dry keratoconjunctivitis.


Assuntos
Artrite Reumatoide , Ceratoconjuntivite , Síndrome de Sjogren , Biomarcadores , Humanos , Prognóstico , Proteína Tirosina Fosfatase não Receptora Tipo 22
3.
Vestn Oftalmol ; 134(3): 108-115, 2018.
Artigo em Russo | MEDLINE | ID: mdl-29953090

RESUMO

The article reviews the results of Russian and foreign studies concerning the search of susceptibility genes for widespread multifactorial diseases: Sjogren's syndrome and rheumatoid arthritis. Studying regularity patterns of genome changes in autoimmune processes with ophthalmic manifestations and their correlation with the severity of dry keratoconjunctivitis will contribute to a better understanding of the etiology and pathogenesis of eye manifestations of the diseases, and will also allow the development of new effective methods of diagnosis and targeted therapy.


Assuntos
Síndromes do Olho Seco , Ceratoconjuntivite , Síndrome de Sjogren , Síndromes do Olho Seco/diagnóstico , Humanos , Prognóstico , Federação Russa
4.
Vopr Virusol ; 46(5): 40-2, 2001.
Artigo em Russo | MEDLINE | ID: mdl-11715709

RESUMO

High antiviral activity of 2'-deoxy-2'fluoroguanosine (2'-D-2'-FG) was observed in chicken embryo cells infected with FPV/Rostock/34 (H7N1) influenza virus and herpes simplex virus (HSV) type I (strain 1C). 50% inhibitory concentration (IC50) of 2'-D-2'-FG was 1.44 microM for FPV and 0.093 microM for HSV. IC50 of remantadine hydrochloride, ribavirin (FPV), and acycloguanosine (HSV) were < 0.46, 14.4, and 0.08 microM, respectively. Anti-HSV activity was confirmed in Vero cells: IC50 < 0.34 microM for 2'-D-2'-FG and IC50 < 0.044 microM for acycloguanosine.


Assuntos
Antivirais/farmacologia , Desoxiguanosina/análogos & derivados , Desoxiguanosina/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Orthomyxoviridae/efeitos dos fármacos , Animais , Embrião de Galinha , Testes de Sensibilidade Microbiana , Células Vero
5.
Aviakosm Ekolog Med ; 34(1): 32-5, 2000.
Artigo em Russo | MEDLINE | ID: mdl-10732192

RESUMO

Effects of space flight on growth and biosynthetic features of plant cells were studied in two strains of ginseng (Panax ginseng) differing in growth and particularly biosynthetic activities, a strain of Lithospermum Erythrorhizon and a strain of Macrotomia Euchroma which produce biologically active naphroquin-derived pigments. --and also differ in growth and biosynthetic properties. Following exposure aboard MIR and a Space shuttle, cells of the callosal cultures were subjected to callosal or suspension passaging. Biomass yield and biologically active substances--ginseng saponins ginsenoids and shikonin were determined in the cells cultures. There was no evidence for the biomass yield to be significantly altered by space flight; however, the content of biologically active substances was materially changed with the strain.


Assuntos
Panax/fisiologia , Plantas Medicinais , Sementes/fisiologia , Voo Espacial , Meio Ambiente Extraterreno , Naftoquinonas/metabolismo , Panax/citologia , Pigmentos Biológicos/biossíntese , Sementes/citologia
6.
Nucleosides Nucleotides ; 18(4-5): 687-8, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10432666

RESUMO

Chemical and enzymatic methods were employed for the synthesis of the title compound, 2'F-Guo 7. High antiviral activity of 2'F-Guo was established in chick embryo cells infected with influenza virus FPV/Rostock/34 (H7N1) and herpes simplex virus (HSV) type I (1C strain).


Assuntos
Antivirais/síntese química , Antivirais/farmacologia , Desoxiguanosina/análogos & derivados , Animais , Embrião de Galinha , Desoxiguanosina/síntese química , Desoxiguanosina/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Orthomyxoviridae/efeitos dos fármacos
8.
FEBS Lett ; 183(2): 275-8, 1985 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-2580738

RESUMO

It is shown that dNTP(3'F) are terminators of DNA synthesis and may serve as very effective tools for DNA sequencing with E.coli DNA polymerase I and AMV reverse transcriptase. The dNTP(3'F) are found to be chain terminator substrates for calf thymus terminal deoxyribonucleotidyl transferase but not for calf thymus DNA polymerase alpha. The optimal dNTP(3'F) concentration for DNA sequencing by DNA polymerase I is found to be an order of magnitude lower than that of ddNTPs. dNTP(3'F) produce a more clear sequence pattern than do ddNTPs.


Assuntos
Replicação do DNA/efeitos dos fármacos , Desoxirribonucleotídeos/farmacologia , Flúor/farmacologia , Animais , Sequência de Bases , Bovinos , DNA Nucleotidilexotransferase/metabolismo , DNA Polimerase I/metabolismo , Escherichia coli , DNA Polimerase Dirigida por RNA/metabolismo
9.
Mikrobiologiia ; 46(4): 707-10, 1977.
Artigo em Russo | MEDLINE | ID: mdl-333238

RESUMO

The effect of nitrosoguanidine (NG) and ultra-violet light (UV) on the survival and the activity of glucoamylase (GIA) was studied with Endomycopsis fibuligera. Variants which strongly differed from the control cultures in their activity of Gia were sported only by the action of NG, particularly at a concentration of 500 mcg/ml, during 90 min. In this case, about 5 per cent of variants with the activity of GIA being 160-200 per cent of the control value appeared in the population.


Assuntos
Ascomicetos/enzimologia , Glucana 1,4-alfa-Glucosidase/metabolismo , Glucosidases/metabolismo , Nitrosoguanidinas/farmacologia , Saccharomycetales/enzimologia , Raios Ultravioleta , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Mutação/efeitos dos fármacos , Mutação/efeitos da radiação , Saccharomycetales/efeitos dos fármacos , Saccharomycetales/efeitos da radiação
10.
Prikl Biokhim Mikrobiol ; 11(5): 682-5, 1975.
Artigo em Russo | MEDLINE | ID: mdl-171644

RESUMO

The effect of glucose and cyclic 3',5'-adenosine monophosphate (cAMP) on the synthesis of glucoamylase and alpha-amylase by the yeast Endomycopsis fibuligera str. 20-9 was investigated. The glucoamylase synthesis was inhibited by every concentration (0.05--3.0%) of glucose. The synthesis of alpha-amylase was significantly reduced by high concentrations (2.0-3.0%) of carbohydrate only. An addition of cAMP (1 X 10(-5) M) reversed compeltely the inhibitory effect of 0.5-2.0% glucose on the alpha-amylase synthesis and partially restored the glucoamylase synthesis. It is concluded that catabolite repression may be involved in the regulation of alpha-amylase and glucoamylase synthesis by Endomycopsis fibuligera str. 20-9 and that this type of regulation shows varying effectiveness in the enzyme synthesis.


Assuntos
Amilases/biossíntese , Ascomicetos/enzimologia , Glucosidases/biossíntese , Saccharomycetales/enzimologia , Meios de Cultura , AMP Cíclico/farmacologia , Relação Dose-Resposta a Droga , Glucose/farmacologia , Mutação , Polissacarídeos , Saccharomycetales/efeitos dos fármacos , Fatores de Tempo
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