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J Conserv Dent ; 24(2): 179-183, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34759586


Aims: This study evaluated the hybrid layer formation and bond strength of two adhesive systems, Scotchbond Universal (U) or Adper Scotchbond Multi Purpose (M), after cleaning protocols using ethanol (E) or xylol (X), to dentin impregnated with an epoxy-resin based endodontic sealer. Settings and Design: The study design was an Experimental in vitro study. Methodology: One hundred bovine dentin specimens were randomly allocated into five groups (n = 10): Computed tomography (CT) (control): Only acid etching + M; E+U; X+U; E+M. After the specimen preparation, images were obtained using confocal laser scanning microscopy to evaluate the hybrid layer formation. For microshear bond strength test, the dentin specimens were included in polyvinyl chloride tubes and four resin composite cylinders were placed on the surface. The analysis was performed 24 h after storage. Statistical Analysis Used: For parametric and nonparametric data, analysis of variance followed by Tukey test and Kruskal-Wallis, followed by Dunn test were, respectively, used at a significance level of 5%. Results: Regarding hybrid layer formation, all experimental groups were similar to each other (P > 0.05). However, CT showed higher hybrid layer formation than other groups (P < 0.05), except in relation to X+M (P > 0.05). Bond strength was statistically similar among all groups (P > 0.05). Conclusions: Hybrid layer formation in dentin impregnated with epoxy resin-based sealer and submitted to different cleaning protocols was similar to the control group only for X+M. No differences were found among the experimental groups. Regarding the bond strength, no effect was observed for any group.

J Clin Exp Dent ; 13(9): e913-e919, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34603620


Background: This study evaluated the effects of several epoxy resin-based sealer compositions (AHP, AH Plus; ADS, Adseal; SPL, Sealer Plus) on bond strength and intratubular dentin penetration of the endodontic obturation, in root canal previously treated with calcium hydroxide intracanal medication (CH) and removed by continuous ultrasonic irrigation (CUI). Material and Methods: Forty-five maxillary canines were prepared up to F5 (ProTaper system), filled with CH, coronally sealed, and stored at 37ºC. After 1 week, CH was removed using 2.5% sodium hypochlorite energized by CUI. The specimens were randomly distributed in three groups (n=15) and root canal obturated, according to epoxy-based resin sealer composition (AHP, ADS or SPL). The roots were transversally sectioned in cervical, middle, and apical thirds. In each radicular third, push out bond strength using universal machine and intratubular dentin using confocal laser scanning microscopy (CLSM) and Image J Program were evaluated. Bond strength and intratubular dentin penetration were statistically evaluated by ANOVA one-way and Tukey tests and Kruskal Wallis test, respectively (α = 0.05). Results: In middle and apical thirds, AHP showed higher bond strength values (p<0.05), and ADS and SPL were similar each other (p>0.05). All epoxy resin-based sealers presented similar intratubular dentin penetration, independently of the radicular thirds (p>0.05). Cohesive and mixed failures were predominant in the cervical thirds. In the middle and apical thirds, AHP showed more cohesive type failures, while ADS and SPL showed more adhesive-type failures. Conclusions: AHP has the highest bond strength in middle and apical radicular thirds, after calcium hydroxide intracanal medication removal using continuous ultrasonic irrigation, although intratubular dentin infiltration being similar among epoxy resin-based sealer with several chemical composition. Key words:Continuous ultrasonic irrigation, endodontic sealers, epoxy resin-based sealers, root canal obturation.

Dental Press J Orthod ; 26(5): e2119350, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34669825


OBJECTIVE: The aim of this in vitro study was to evaluate the antimicrobial effect of five types of non-industrialized and industrialized probiotics on biofilms formed around orthodontic mini-implants. The null hypothesis tested was: there is no difference in the antimicrobial effect between the five types of probiotics tested around orthodontic mini-implants. METHODS: For the experiment, 120 mini-implants were immersed for seven days in Staphylococcus aureus solution for biofilm formation, and were subsequently plated in culture medium containing probiotics. The mini-implants were divided into six different groups, according to the probiotic used: G1)Lactobacillus casei; G2)Lactobacillus brevis; G3)Lactobacillus rhamnosus; G4) Lactobacillus from fermented milk Yakult®; G5) Lactobacillus from fermented milk Batavito® and G6) without use of probiotic, as negative control. Qualitative and quantitative analyses of all groups were performed using the CFU (colony forming unit) count. RESULTS: The study showed that groups G4 and G6 did not present antimicrobial activity, in comparison to groups G1, G2, G3, and G5 (p< 0.05), which demonstrated antimicrobial activity. CONCLUSION: The non-commercial probiotic bacteria, Lactobacillus casei and Lactobacillus rhamnosus, as well as commercially available fermented milk Batavito® presented promising results in the reduction of colonization of mini-implants by S. aureus. Therefore, the null hypothesis was rejected.

Anti-Infecciosos , Implantes Dentários , Procedimentos de Ancoragem Ortodôntica , Probióticos , Staphylococcus aureus
Photodiagnosis Photodyn Ther ; 36: 102511, 2021 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-34469795


BACKGROUND: The association of low concentration hydrogen peroxide (HP) and a light source has been widely used to achieve efficient bleaching. We investigated the colorimetric and microstructural changes of bovine enamel bleached with 6% HP associated or not with a hybrid light source system of violet light and laser (LED/laser). METHODS: Twenty bovine crowns were used to obtain specimens of 7 × 7 × 2 mm. Then, they were randomized in two groups (n=10): 6% HP and 6% HP + LED/laser. After staining with dark tea solution, three bleaching sessions were performed. Colorimetric evaluation (∆L*, ∆a*, ∆b*, ∆E00 [CIEDE2000] and WID) after 24 hours of each session and 7 days after the final bleaching session was performed. Enamel Vickers microhardness at baseline, 24 hours and 7 days after the last bleaching session were also evaluated. Two-way repeated measures ANOVA and Bonferroni post-test was used at a significance level of 5%. RESULTS: 6% HP and 6% + HP LED/laser showed satisfactory bleaching results. The group photoactivated showed higher WID values (p<0.05). Differences between groups were observed for ∆E00, ∆L* and ∆a* (p<0.05), except for ∆b* (p>0.05). Intra-group differences were also found (p<0.05). Regarding microhardness, no inter or intra-group differences were observed (p>0.05). CONCLUSIONS: The photoactivation with LED/laser enhanced the whitening efficacy of 6% HP compared to the group without photoactivation. Thus, the LED/laser activation appears to be a good option when using low concentration HP-based agents. In addition, both bleaching protocols did not cause changes on enamel microhardness.

J Clin Exp Dent ; 13(6): e564-e571, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34188762


Background: The aim of this study was to evaluate the effects of surface treatments with 1% peracetic acid (PA), solution containing 17% EDTA (SmearClear, Kerr Endodontics), solution containing a combination of 17% EDTA with 2% chlorhexidine (QMix, Dentsply Sirona) on the post-space root dentin compared to 2.5% sodium hypochlorite (NaOCl) on bond strength and resin tags length in dentin. Material and Methods: Forty human-canine roots were endodontically treated and the post space was prepared. The specimens were randomised into four groups (n = 10): control - irrigation with 2.5% NaOCl solution, PA - irrigation with 1% PA, SmearClear - irrigation with SmearClear solution, and QMix - irrigation with QMix solution. The fibre posts were cemented using a self-adhesive resin system (Relyx U200, 3M ESPE). After six months, the specimens were cross-sectioned and subjected to push-out and confocal laser microscopy tests. One-way ANOVA and Tukey's tests were used to analyse the data (α= 0.05). Results: PA and QMix presented the highest bond strength values compared to the other groups (p< 0.05). There was no significant difference between the resin tags length in dentin by the groups (p = 0.75). Conclusions: Irrigation of the post space with 1% PA and QMix showed a positive clinical impact on the adhesion between the fiber post and root dentin. However, these materials had no influence on resin tags length in dentin by self-adhesive resin cement. Key words:Fiber post, adhesive cementation, self-adhesive resin cement, root dentin, irrigating solutions.

Microsc Res Tech ; 84(10): 2351-2360, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33960585


This study evaluated the persistence of eugenol-containing (PR, Provy) or eugenol-free (RT, Relyx Temp, and TB, Temp Bond) temporary cement residues and its effects on push-out bond strength and dentinal penetration of the self-adhesive resin cement (Relyx U200). Eighty human roots were endodontically treated and post space prepared. Fourty specimens were distributed in four groups (n = 10): Control (CO), without any clinical procedure, PR, RT, and TB, where a metallic post was cemented with one of the temporary cements. After metallic post removal, hemi sections post space were submitted to SEM analysis. Another specimens were distributed in similar groups, but fiberglass post was cemented using Relyx U200 cement and submitted to push-out bond strength and dentinal penetration cement analysis, in post space thirds. SEM analysis were evaluated by Kruskal-Wallis and Dunn tests, while bond strength and dentinal penetration were evaluated by ANOVA One-Way and Tukey test (p < .05). Only in apical thirds, PR, RT, and TB showed higher persistence of residues than CO. In bond strength, PR, RT, and TB showed lower values than CO, in all post space thirds (p < .05). But to dentinal penetration, PR, RT, and TB presented lower values than CO only in apical thirds (p < .05). There is the greatest persistence of temporary cement residues only in apical post space third, providing less dentinal penetration of the self-adhesive resinous cement. However, the previous use of temporary cements has negative effects on the bond strength cementation system, regardless of the third evaluated.

Cimentação , Cimentos de Resina , Adesivos , Dentina , Vidro , Humanos
Int J Prosthodont ; 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33651023


PURPOSE: To analyze the current evidence on the impact of surface finishing protocols (such as manual polishing or glazing) on the color stability of in vitro simulated pigmentation of CAD/CAM ceramics. MATERIALS AND METHODS: Five electronic databases were searched on February 12, 2020. In vitro experimental studies were included based on the following strategy: intervention = surface treatment of CAD/CAM ceramics with glazing agents; comparison = surface treatment of CAD/CAM ceramics with manual polishing; and outcomes = color stability. Two reviewers independently assessed the risk of bias. RESULTS: Among 1,390 articles that were screened, 6 in vitro studies were considered for qualitative analysis. Five articles confirmed changes in the color of ceramics when they were immersed in pigmented solutions. One article investigated the color stability of the samples via ultraviolet (UV) aging. Among the 6 studies analyzed, 4 showed clinically acceptable color alteration values represented by ΔE irrespective of the finishing protocol applied (glazing or mechanical polishing). Two articles presented clinically unsatisfactory color variation (ΔE > 3.3 and ΔE > 2.7) following mechanical polishing of a zirconia-reinforced lithium silicate ceramic. Coffee and red wine proved to be the beverages with the greatest potential for ceramic pigmentation. Only 1 article had a high risk of bias. CONCLUSION: For clinicians, most of the studies demonstrated that both manual polishing and glaze application can prevent significant color alterations on CAD/CAM ceramic surfaces. However, due to the relatively limited amount of evidence to support this conclusion, further studies must be conducted.

Int. j. odontostomatol. (Print) ; 14(1): 60-66, mar. 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1056502


ABSTRACT: The objective of the study was to evaluate marginal adaptation of Class II mesial-occlusal-distal (MOD) restorations before and after thermo-mechanical loading and volumetric shrinkage of the bulk-fill vs conventional composite resin. For marginal adaptation assessment, 24 Class II MOD cavities with cervical margins extending 1.0 mm below (distal) and 1.0 mm beyond (mesial) the cement-enamel junction were prepared in extracted human molars. The teeth were filled as follows: Group A - bulk-fill with SureFil SDR flow (first increment, 4 mm; second increment, 2 mm); Group B - bulk-fill with SureFil SDR flow as a base (first increment, 4 mm) and covered with the conventional nanohybrid composite Esthet-X HD (second increment, 2 mm); and Group C - incrementally filled with Esthet-X HD. Marginal adaptation was evaluated by scanning electron microscopy before and after thermomechanical loading (240,000 loading cycles and simultaneous 600 thermal cycles). To evaluate volumetric polymerization shrinkage, a semi-spherical mold was filled with the tested composites and placed in an AccuVol device after light curing. Both before and after loading, marginal adaptation in cervical dentin was superior (p < 0.05) for Groups A and B compared with Group C. In cervical enamel, Group B showed better marginal adaptation than Group C, and Group A presented intermediary results, between Groups B and C. Furthermore, bulk-fill flow resulted in greater shrinkage than Esthet-X HD. A significant improvement of marginal adaptation was observed when bulkfill flow was used instead of conventional composite resin both before and after thermomechanical loading. However, the bulk-fill flow presented higher volumetric polymerization shrinkage than the conventional composite.

RESUMEN: El objetivo de este trabajo consistió en evaluar la adaptación marginal de las restauraciones mesiales-oclusales-distales (MOD) de Clase II antes y después de la carga termo-mecánica y la contracción volumétrica de la carga compuesta de resina "bulk-fill" en comparación con resina convencional. Para la evaluación de adaptación, se prepararon 24 cavidades MOD de Clase II en molares humanos extraídos, los que se restauraron de la siguiente manera: Grupo A: restaurado con resina fluida Bulk-Fill SureFilSDR (primer incremento, 4 mm; segundo incremento, 2 mm); Grupo B: restaurado con resina fluida Bulk-Fill SureFil SDR (primer incremento, 4 mm) y cubierto con resina compuesta nanohíbrida Esthet-X HD (segundo incremento, 2 mm); y Grupo C - rellenado incrementalmente con Esthet-X HD. La adaptación marginal se evaluó mediante microscopía electrónica de barrido antes y después de la carga termomecánica (240.000 ciclos de carga y 600 ciclos térmicos simultáneos). Para evaluar la contracción volumétrica de la polimerización, se llenó un molde semiesférico con los compuestos probados y se colocó en un dispositivo AccuVol después del fotopolimerización. Tanto antes como después de la carga, la adaptación marginal en la dentina cervical fue superior (p <0,05) para los grupos A y B en comparación con el grupo C. En el esmalte cervical, el grupo B mostró una mejor adaptación marginal que el grupo C, y el grupo A presentó resultados intermedios, entre Grupos B y C. Se observó una mejora significativa de la adaptación marginal al utilizar la resina fluida Bulk-Fill en lugar de resina compuesta convencional tanto antes como después de la carga termomecánica. Sin embargo, la resina fluida 'Bulk-Fill' presentó una mayor contracción volumétrica de polimerización que el compuesto convencional.

Humanos , Adaptação Marginal Dentária , Resinas Compostas , Preparo da Cavidade Dentária/instrumentação , Restauração Dentária Permanente/métodos , Má Oclusão Classe II de Angle , Estresse Mecânico , Análise Estatística , Comissão de Ética , Desgaste de Restauração Dentária , Preparo da Cavidade Dentária/normas , Polimerização