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1.
Braz Oral Res ; 34: e006, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32022225

RESUMO

Induced pluripotent stem (iPS) cells could be induced into ameloblast-like cells by ameloblasts serum-free conditioned medium (ASF-CM), and bone morphogenetic proteins (BMPs) might be essential during the regulation of this process. The present study investigates the signal transduction that regulates the ameloblastic differentiation of iPS cells induced by ASF-CM. Mouse iPS cells were characterized and then cultured for 14 days in epithelial cell medium (control) or ASF-CM. Bone morphogenetic protein receptor II (BMPR-II) siRNA, inhibitor of Smad1/5 phosphorylation activated by activin receptor-like kinase (ALK) receptors, and inhibitors of mitogen-activated protein kinases (MAPKs) phosphorylation were used to treat the iPS cells in combination with ASF-CM. Real-time PCR, western blotting, and immunofluorescent staining were used to evaluate the expressions of ameloblast markers ameloblastin, enamelin, and cytokeratin-14. BMPR-II gene and protein levels increased markedly in ASF-CM-treated iPS cells compared with the controls, while the mRNA levels of Bmpr-Ia and Bmpr-Ib were similar between the ASF-CM and control groups. ASF-CM stimulation significantly increased the gene and protein expression of ameloblastin, enamelin and cytokeratin-14, and phosphorylated SMAD1/5, p38 MAPK, and ERK1/2 MAPK compared with the controls. Knockdown of BMPR-II and inhibition of Smad1/5 phosphorylation both could significantly reverse the increased expression of ameloblastin, enamelin, and cytokeratin-14 induced by ASF-CM, while neither inhibition of p38 nor ERK1/2 phosphorylation had significant reversing effects. We conclude that smad1/5 signaling transduction, activated by ALK receptors, regulates the ameloblastic differentiation of iPS cells induced by ameloblast-conditioned medium.

2.
Ying Yong Sheng Tai Xue Bao ; 31(1): 275-281, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31957405

RESUMO

Japanese Spanish mackerel (Scomberomorus niphonius) is one of the main economic fish species in the Yellow Sea. To understand its spawning habitat and population dynamics in the early stage, we examined the distribution and environmental characteristics of spawning ground of Japanese Spanish mackerel using their egg survey data collecting in 2015 (late May) and 2017 (late May) in the coastal waters of Yellow Sea. The generalized additive model (GAM) with the Tweedie distribution was used to analyze the distribution and environmental characteristics of its spawning ground. The results showed that egg distribution was significantly related to the location (i.e., latitude and longitude), water depth, and sea surface temperature (SST), but not related to sea surface salinity (SSS). The optimal range of depth and SST were 15-30 m and 16-20 ℃, respectively. The mackerel spawning ground mainly distributed in the region Shidao fishing ground (area near 36°30' N,121°48' E) and Haizhou Bay (33°30'-36° N,119°30'-121°30' E). There were some clear inter-annual differences in the distribution of mackerel spawning ground, with the spawning ground tending to northward in the year with higher sea temperature. Therefore, it is necessary to manage and protect the above spawning ground (especially the Haizhou Bay), establishing the corresponding ecological protection and restoration strategy, to realize sustainable exploitation of mackerel fisheries resources in the Yellow Sea.


Assuntos
Perciformes , Animais , China , Ecossistema , Pesqueiros , Dinâmica Populacional
3.
Clin Chim Acta ; 502: 55-65, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31821791

RESUMO

BACKGROUND: Urinary proteins could be useful as markers for the detection of non-small-cell lung cancer (NSCLC). We investigated the levels of two different proteins in urine samples from NSCLC patients and assessed their diagnostic value. METHODS: Urinary plasminogen (PLG) and fibrinogen gamma chain (FGG) levels in 112 NSCLC patients and 197 controls were detected using enzyme linked immunosorbent assay (ELISA). The expression of FGG and PLG in 20 NSCLC tissues and paired adjacent non-tumour tissues were detected through immunohistochemistry. The diagnostic value of FGG and PLG for NSCLC was evaluated through a receiver operating characteristic curve (ROC). RESULTS: PLG and FGG were significantly elevated in NSCLC tissues vs paired adjacent non-tumour tissues (p = 0.000) and in urinary samples from NSCLC patients vs healthy controls (p = 0.000). The expression level of PLG in urinary samples was related only to the histological type (p = 0.001). Further, ROC curve analysis revealed that PLG, FGG, and their combination could distinguish NSCLC and its subtypes from healthy controls with an AUC ranging from 0.827 to 0. 947. By comparing urine samples with matching plasma CEA from NSCLC stage I-IV patients (n = 81) and healthy controls (n = 31), the combination of CEA with PLG or FGG showed that the AUC was 0.889 and 0.806, respectively, which is superior to a single biomarker alone. CONCLUSIONS: These two urinary proteins could serve as potential markers for the diagnosis of NSCLC.

4.
J Hazard Mater ; 384: 121470, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31648892

RESUMO

Steam-activated biochar (SBC) was prepared and showed excellent performance for synergistic removal of Cu2+ and tetracycline (TC). The adsorption capacity of SBC and mutual effect of TC and Cu2+ were investigated via single and binary system and the adsorption isotherm. The adsorption capacity of TC was significantly enhanced when it coexisted with Cu2+. Likewise, increased amounts of Cu2+ were adsorbed in the presence of TC. The presence of NaCl exerted a negative influence on the adsorption of Cu2+, while the inhibitory effect of salinity on TC was neutralized by bridge enhancement in the binary system. Bridge enhancement and site competition were involved in the synergistic removal of TC and Cu2+. Considering the stable application in simulated and real water samples, SBC showed great potential for synergistic removal of antibiotics and heavy metals.

5.
IEEE Trans Biomed Eng ; 67(2): 353-364, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31034402

RESUMO

OBJECTIVE: Technical skill assessment plays an important role in the professional development of an interventionalist in percutaneous coronary intervention (PCI). However, most of the traditional assessment methods are time consuming and subjective. This paper aims to develop objective assessment techniques. METHODS: In this study, a natural-behavior-based assessment framework is proposed to qualitatively and quantitatively assess technical skills in PCI. In vivo porcine studies were conducted to deliver a medical guidewire to two target coronaries of left circumflex arteries by six novice and four expert interventionalists. Simultaneously, four types of natural behaviors (i.e., hand motion, proximal force, muscle activity, and finger motion) were acquired from the subjects' dominant hand and arm. The features extracted from the behaviors of different skill-level groups were compared using the Mann-Whitney U-test for effective behavior selection. The effective ones were further applied in the Gaussian-mixture-model-based qualitative assessment and Mahalanobis-distance-based quantitative assessment. RESULTS: The qualitative assessment achieves an accuracy of 92% to distinguish the novice and expert attempts, which is significantly higher than that of using single guidewire motions. Furthermore, the quantitative assessment can assign objective and effective scores for all attempts, indicating high correlation ( R = 0.9225) to those obtained by traditional methods. CONCLUSION: The objective, effective, and comprehensive assessment of technical skills can be provided by qualitatively and quantitatively analyzing interventionalists' natural behaviors in PCI. SIGNIFICANCE: This paper suggests a novel approach for the technical skill assessment and the promising results demonstrate the great importance and effectiveness of the proposed method for promoting the development of objective assessment techniques.

6.
J Cancer ; 10(27): 6779-6791, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31839812

RESUMO

Purpose. To investigate the association of biomarkers correlated with clinical stages and survival of clear cell renal cell carcinoma (ccRCC). Methods. The GSE36895 dataset was downloaded and differentially expressed or methylated genes were analyzed. Hub genes were identified with weighted gene co-expression network analysis (WGCNA) and protein-protein interaction network (PPI), and validated with TCGA database and our own tissues. The biological processes of hub genes were further explored by functional enrichment analysis. Survival analyses were also performed. The underlying mechanisms for ccRCC development were detected with Gene set enrichment analyses. Results. A total of 1624 differentially expressed genes were analyzed by WGCNA and 6 co-expressed gene modules were identified. Three hub genes (EHHADH, ACADM and AGXT2) were met the criterion of both WGCNA and PPI networks analysis, which showed highest negative association with pathological T stage (r = - 0.45, p = 0.01) and tumor grade (r = - 0.45, p = 0.01). The downregulation of these hub genes was validated with using both TCGA database and samples harvested at our institute The biological processes that hub genes involved, such as metabolic process (p = 9.63E - 09), oxidation-reduction process (p = 1.05E - 08) and oxidoreductase activity (p = 1.72E - 04), were revealed. Survival analysis showed a higher expression or lower methylation of these hub genes, a longer survival of ccRCC patients. ccRCC samples with higher expression of hub genes were enriched in gene sets correlated with signaling like biosynthesis of unsaturated fatty acids, butanoate metabolism, and PPAR signaling pathway. Conclusions. We identified three novel tumor suppressors associated with pathological T stage and overall survival of ccRCC. They might be potential as individualized therapeutic targets and diagnostic biomarkers for ccRCC.

7.
Ying Yong Sheng Tai Xue Bao ; 30(11): 3787-3794, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31833692

RESUMO

To unravel functional leaf photosynthetic mechanisms underlying enhanced yield of sesame in sesame/peanut intercropping, a field experiment was carried out in 2017 and 2018 with four treatments including (1) three-row sesame intercropped with six-row peanut (IC 3:6), (2) two-row sesame intercropped with four-row peanut (IC 2:4), (3) sole cropped sesame (SS), and (4) sole cropped peanut (SP). We measured the parameters of gas exchange, characteristics of photosynthetic response curve to light and CO2, and characteristics of chlorophyll rapid fluorescence induction kinetic curves of the functional leaves of sesame. The results showed that the partial land equivalent ratio of intercropped sesame was greater than 1/3. The light saturation point (Isat), maxi-mum net photosynthetic rate (Pn max), maximum electron transport rate (Jmax), triose phosphate utilization rate (TPU), maximum carboxylation rate of Rubisco (Vc max) were increased significantly under intercropping. Further, absorption energy flux per CS (ABS/CSo), trapping energy flux per CS (TRo/CSo), number of active reaction centers per CS (RC/CSm), and electron transport flux per CS (ETo/CSo) in intercropped treatments were enhanced compared to that under sesame monoculture. However, the ratio between variable fluorescence Fk to amplitude Fj-Fo (Wk) and ratio between variable fluorescence Fj to amplitude Fp-Fo (Vj) in functional leaves of intercropped sesame were significantly decreased. The efficiency of converting light energy into electricity of PS2 reaction center (Ψo), electron transfer efficiency from PS2 to end acceptor of PS1 (ΨRo), electron transfer efficiency of the electron transport chain (δR), PS1 photochemical activity, and the coordination between PS2 and PS1 in functional leaves of intercropped sesame were increased. The net photosynthetic rate (Pn), stomatal conductance (gs), transpiration rate (Tr), Pn max, Jmax, Vc max, TPU, Ψo, ΨRo and δR were significantly higher in IC 3:6 than those in IC 2:4. We conclude that intercropping improves net photosynthetic rate and yield of sesame by increasing light absorption, electronic transmission, activity of PS2 donator/receptor sides, and CO2 fixation, with stronger effects in IC 3:6 than IC 2:4.


Assuntos
Arachis , Sesamum , Clorofila , Fluorescência , Fotossíntese , Folhas de Planta
8.
Inorg Chem ; 58(23): 15898-15908, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31724839

RESUMO

A series of novel metal-organic frameworks were synthesized by using semirigid ligand 1,1',1″-(1,3,5-triazine-2,4,6-triyl)tripiperidine-4-carboxylic acid (H3TTPCA) and lead halide (Cl, Br, or I). The three complexes were characterized by elemental analysis, infrared spectroscopy, ultraviolet-visible spectroscopy, powder X-ray diffraction analysis, and thermogravimetric analysis. X-ray single-crystal diffraction analysis demonstrated that all three complexes were three-dimensional inorganic-organic framework structures with Pb-X2 (X = Cl, Br, or I). However, slight differences in the chemical environment were the focus of the coordinated halogen atoms and the different compositions of metal oxygen clusters: [Pb7(COO)12Cl2], [Pb7(COO)12Br2], and [Pb7(COO)12I2]. Because of the fluorescence of the organic ligand, the three complexes showed similar photoluminescence properties at room temperature, but the intensity of emissions decreased gradually with an increase in the atomic radius of coordinated halogen atoms. Interestingly, in the fluorescence response tests, complexes 1 and 2 displayed an optical signal of fluorescence "turn-on" while complex 3 showed an optical signal of fluorescence "turn-off". Here we aim to provide a possible mechanism to explain these unique and contradictory luminescence results.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31746303

RESUMO

BACKGROUND: Although previous research has shown that internalizing and externalizing behavior problems often co-occur, the relationship between the developmental trajectories of these two types of behavior problems is understudied. The co-occurring evolutions of developmental trajectories of two behaviors has two components: 1) the correlation between the slopes of two behavior profiles (termed the association of the evolutions); and 2) the marginal correlation of two development trajectory profiles, which is the development of correlation between internalizing and externalizing behavior over time (termed the evolution of the association). The association of the evolutions and the evolution of the association have not been not fully explored in the context of the development of internalizing and externalizing behavior problems among kindergarteners in the United States. METHODS: The random-effects approach for joint modeling of multivariate longitudinal profiles was used to evaluate the co-development and its temporal pattern of internalizing and externalizing behavior problems on a nationally representative sample of 9791 kindergarteners from the Early Childhood Longitudinal Study-Kindergarten Class of 1998-99 (ECLS-K). RESULTS: There was a moderate positive association between the evolutions of the two behavior problems with correlation coefficient of 0.319. The evolution of association between the two behaviors was increasing over time with the correlation coefficient from 0.195 at the Fall of kindergarten to 0.291 by the time of fifth grade in general. Race and age groups act differently on the evolution of association. The associations were getting stronger for the Asian group and older groups than their peer groups. CONCLUSION: This investigation of the association of evolutions and the evolution of association between the internalizing and externalizing behaviors show that the two problem behaviors reciprocally reinforce each other and lead to increases in the other in a moderate strength and the strength is increasing over time.

10.
Sensors (Basel) ; 19(22)2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31739400

RESUMO

We present an innovative approach for a Cybersecurity Solution based on the Intrusion Detection System to detect malicious activity targeting the Distributed Network Protocol (DNP3) layers in the Supervisory Control and Data Acquisition (SCADA) systems. As Information and Communication Technology is connected to the grid, it is subjected to both physical and cyber-attacks because of the interaction between industrial control systems and the outside Internet environment using IoT technology. Often, cyber-attacks lead to multiple risks that affect infrastructure and business continuity; furthermore, in some cases, human beings are also affected. Because of the traditional peculiarities of process systems, such as insecure real-time protocols, end-to-end general-purpose ICT security mechanisms are not able to fully secure communication in SCADA systems. In this paper, we present a novel method based on the DNP3 vulnerability assessment and attack model in different layers, with feature selection using Machine Learning from parsed DNP3 protocol with additional data including malware samples. Moreover, we developed a cyber-attack algorithm that included a classification and visualization process. Finally, the results of the experimental implementation show that our proposed Cybersecurity Solution based on IDS was able to detect attacks in real time in an IoT-based Smart Grid communication environment.

11.
Atmos Chem Phys Discuss ; 19(15): 10087-10110, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31632450

RESUMO

We have derived values of the Ultraviolet Index (UVI) at solar noon using the Tropospheric Ultraviolet Model (TUV) driven by ozone, temperature and aerosol fields from climate simulations of the first phase of the Chemistry-Climate Model Initiative (CCMI-1). Since clouds remain one of the largest uncertainties in climate projections, we simulated only the clear-sky UVI. We compared the modelled UVI climatologies against present-day climatological values of UVI derived from both satellite data (the OMI-Aura OMUVBd product) and ground-based measurements (from the NDACC network). Depending on the region, relative differences between the UVI obtained from CCMI/TUV calculations and the ground-based measurements ranged between -5.9% and 10.6%. We then calculated the UVI evolution throughout the 21st century for the four Representative Concentration Pathways (RCPs 2.6, 4.5, 6.0 and 8.5). Compared to 1960s values, we found an average increase in the UVI in 2100 (of 2-4%) in the tropical belt (30°N-30°S). For the mid-latitudes, we observed a 1.8 to 3.4 % increase in the Southern Hemisphere for RCP 2.6, 4.5 and 6.0, and found a 2.3% decrease in RCP 8.5. Higher increases in UVI are projected in the Northern Hemisphere except for RCP 8.5. At high latitudes, ozone recovery is well identified and induces a complete return of mean UVI levels to 1960 values for RCP 8.5 in the Southern Hemisphere. In the Northern Hemisphere, UVI levels in 2100 are higher by 0.5 to 5.5% for RCP 2.6, 4.5 and 6.0 and they are lower by 7.9% for RCP 8.5. We analysed the impacts of greenhouse gases (GHGs) and ozone-depleting substances (ODSs) on UVI from 1960 by comparing CCMI sensitivity simulations (1960-2100) with fixed GHGs or ODSs at their respective 1960 levels. As expected with ODS fixed at their 1960 levels, there is no large decrease in ozone levels and consequently no sudden increase in UVI levels. With fixed GHG, we observed a delayed return of ozone to 1960 values, with a corresponding pattern of change observed on UVI, and looking at the UVI difference between 2090s values and 1960s values, we found an 8 % increase in the tropical belt during the summer of each hemisphere. Finally we show that, while in the Southern Hemisphere the UVI is mainly driven by total ozone column, in the Northern Hemisphere both total ozone column and aerosol optical depth drive UVI levels, with aerosol optical depth having twice as much influence on the UVI as total ozone column does.

12.
Cell Death Dis ; 10(11): 789, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31624237

RESUMO

This study was designed to investigate the mechanism by which miR-129-5p affects the biological function of liver cancer cells. The expression levels of miR-129-5p in liver cancer tissues and cells were, respectively, determined. Crystal violet staining and flow cytometry were used to detect cell proliferation and apoptosis. Wound healing assay and transwell assay were performed to test cell migration and invasion. The target gene of miR-129-5p was analyzed and verified by bioinformatics analysis and luciferase reporter assay. Tumorigenicity assays in nude mice were used to test the antitumor ability of calcium calmodulin-dependent protein kinase IV (CAMK4). miR-129-5p was found to be underexpressed in hepatocellular cancer tissues and cells and also to inhibit liver cells proliferation, migration, and invasion and promote apoptosis. CAMK4 was a direct target for miR-129-5p and was lowly expressed in liver cancer tissues and cells. CAMK4 was also found to inhibit liver cells proliferation, migration and invasion, and promote apoptosis. CAMK4 might exert an antitumor effect by inhibiting the activation of mitogen-activated protein kinase (MAPK). MiR-129-5p was a tumor suppressor with low expression in liver cancer tissues and cells. CAMK4, which is a direct target gene of miR-129-5p, could inhibit tumor by inhibiting the activation of MAPK signaling pathway.

13.
Biochem Biophys Res Commun ; 519(3): 533-539, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31537387

RESUMO

Currently, there is no effective treatment for Burkitt's lymphoma in patients aged above 60 years, and thus research on effective treatment options for Burkitt's lymphoma has been gaining increasing attention. Artesunate has been identified as a novel effective growth suppressor in Burkitt's lymphoma. Here, we utilized molecular biology, transcriptome analysis, and other techniques to study artesunate-induced death of the Burkitt's lymphoma cells DAUDI and CA-46, the effect of artesunate on gene expression in DAUDI and CA-46 cells, and the effect of artesunate-induced ATF4-CHOP-CHAC1 pathway on ferroptosis. We also studied the inhibitory effects and ferroptosis induction of artesunate on CA-46 cells in mouse xenografts. Results showed that artesunate induced ferroptosis in DAUDI and CA-46 cells, as evidenced by the protective effect of liproxstatin-1, ferrostatin-1, and desferoxamine, resulting in an endoplasmic reticulum stress response, activation of the ATF4-CHOP-CHAC1 pathway enhanced ferroptosis in DAUDI and CA-46 cells. A mouse-transplanted tumor model showed that artesunate can inhibit the proliferation and induce ferroptosis of CA-46 cells in vivo. This study provides a novel perspective for the development of drugs against different types of Burkitt's lymphomas.

14.
Chemosphere ; 237: 124517, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31549644

RESUMO

The dye wastewater treatment by membrane separation technology has obtained extensive attention in recent years. Nevertheless, it was rare for research on the removal of differently charged mixed dyes. In this study, several UiO-66-NH2 composite membranes were prepared and optimization experiments were conducted. The performance of composite membranes were evaluated by the removal of cationic (Methylene blue, MB), neutral (Rhodamine B, RB), and anionic (Congo red, CR) dyes. The optimization results demonstrated that the UiO-66-NH2/graphene oxide (UNG) composite membrane (PUF/PDA/UNG) which was loaded on polyurethane foam modified with polydopamine (PUF/PDA) had the best properties. In filtration experiments, the solution pH exhibited greater effect on the removal efficiency of MB and CR than RB. When NaCl, KCl, CaCl2 and Na2SO4 coexisted in the dye solution, the removal efficiency of MB by PUF/PDA/UNG membrane were 96.62%, 98.17%, 86.39% and 99.34% respectively. The presence of humic acid showed slight inhibitory effect on the removal of MB by PUF/PDA/UNG membrane (71.93%). The experimental results for mixed dyes filtration showed that PUF/PDA/UNG membrane could effectively remove MB, RB and CR in binary (i.e., MB/RB and RB/CR) and ternary (i.e., MB/RB/CR) systems through secondary filtration. And PUF/PDA/UNG membrane could remove MB and CR simultaneously through one-time filtration in MB/CR binary system. The removal mechanism was mainly attributed to the aggregation of mixed dyes, electrostatic interaction between dye molecules and the membrane surface, and hydrogen bonding. All results suggested that the as-prepared PUF/PDA/UNG membrane have great potential in practical treatment of dye wastewater.


Assuntos
Corantes/química , Grafite/química , Eliminação de Resíduos Líquidos , Poluentes Químicos da Água/química , Adsorção , Filtração , Indóis , Membranas , Azul de Metileno , Polímeros , Poliuretanos , Rodaminas , Águas Residuárias
15.
Angew Chem Int Ed Engl ; 58(43): 15435-15440, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31448499

RESUMO

Low-dimensional lead halide perovskite materials recently have drawn much attention owing to the intriguing broadband emissions; however, the toxicity of lead will hinder their future development. Now, a lead-free (C4 H14 N2 )2 In2 Br10 single crystal with a unique zero-dimensional (0D) structure constituted by [InBr6 ]3- octahedral and [InBr4 ]- tetrahedral units is described. The single crystal exhibits broadband photoluminescence (PL) that spans almost the whole visible spectrum with a lifetime of 3.2 µs. Computational and experimental studies unveil that an excited-state structural distortion in [InBr6 ]3- octahedral units enables the formation of intrinsic self-trapped excitons (STEs) and thus contributing the broad emission. Furthermore, femtosecond transient absorption (fs-TA) measurement reveals that the ultrafast STEs formation together with an efficient intersystem crossing has made a significant contribution to the long-lived and broad STE-based emission behavior.

16.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1829-1835, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31342709

RESUMO

The chemical constituents from methanol extract of Dichroa hirsuta were separated by silica gel and Sephadex LH-20 column chromatography,high pressure preparative liquid chromatography( HPLC) and recrystallization. Their structures were elucidated by NMR and MS. Nine compounds were obtained and their structures were identified as 3ß,21α-O-diacetyl-lup-9( 11)-en-7ß-ol( 1),( Z)-methyl p-hydroxycinnamate( 2),cis-p-coumaric acid ethyl ester( 3),( E)-methyl p-hydroxycinnamate( 4),trans-p-coumaric acid ethyl ester( 5),4( 3 H)-quinazolinone( 6),7-hydroxycoumarin( 7),hydrangenol( 8) and thunberginol C( 9). Compound 1 is a new lupane-type triterpenoid,and compounds 1-5,8-9 were firstly isolated from this plant. Dual reporter assay results showed that compounds 2-5 could activate the Nrf2-ARE signaling pathway.


Assuntos
Medicamentos de Ervas Chinesas , Hydrangea/química , Triterpenos/farmacologia , Cromatografia Líquida de Alta Pressão , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Triterpenos/isolamento & purificação
17.
Front Psychol ; 10: 1457, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31333530

RESUMO

Given the enormous population of Chinese-speaking people worldwide, it is important to establish measures of adolescent wellbeing with adequate evidence for reliability and validity. The EPOCH Measure of Adolescent Wellbeing assesses five positive psychological characteristics (engagement, perseverance, optimism, connectedness, and happiness). An initial study with the English version of the measure found support for a five-factor structure, and evidence for internal reliability, convergence with other wellbeing measures, and divergence across factors and with unrelated constructs. An initial study translated the measure into Chinese and found support for the factor structure of the measure. To further test the measure's psychometric properties, data were collected from 11 Chinese student samples (N = 17,854) from several regions of China. All students completed the EPOCH measure, along with a variety of other measures. In cases where measures overlapped, samples were combined, with relevant sub-sets used to examine convergent and divergent patterns. Confirmatory analyses supported the five-factor structure and factors were internally reliable, but consistency over time was low. The five factors were more strongly correlated with other wellbeing factors than with illbeing factors. While some correlations demonstrated expected convergent and divergent patterns with other constructs, there were also considerable deviations from expected patterns. Norm values specific to the Chinese version of the measure are provided. The study supports the EPOCH measure as a useful cross-sectional tool for measuring adolescent positive functioning, but additional consideration of cross time stability, change, and correlations with other constructs is needed.

18.
Sensors (Basel) ; 19(14)2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31319562

RESUMO

In the age of the Internet of Things, connected devices are changing the delivery system in the healthcare communication environment. With the integration of IoT in healthcare, there is a huge potential for improvement of the quality, safety, and efficiency of health care in addition to promising technological, economical, and social prospects. Nevertheless, this integration comes with security risks such as data breach that might be caused by credential-stealing malware. In addition, the patient valuable data can be disclosed when the perspective devices are compromised since they are connected to the internet. Hence, security has become an essential part of today's computing world regarding the ubiquitous nature of the IoT entities in general and IoT-based healthcare in particular. In this paper, research on the algorithm for anonymizing sensitive information about health data set exchanged in the IoT environment using a wireless communication system has been presented. To preserve the security and privacy, during the data session from the users interacting online, the algorithm defines records that cannot be revealed by providing protection to user's privacy. Moreover, the proposed algorithm includes a secure encryption process that enables health data anonymity. Furthermore, we have provided an analysis using mathematical functions to valid the algorithm's anonymity function. The results show that the anonymization algorithm guarantees safety features for the considered IoT system applied in context of the healthcare communication systems.


Assuntos
Técnicas Biossensoriais , Assistência à Saúde/tendências , /tendências , Algoritmos , Comunicação , Humanos
19.
Cancer Biomark ; 25(4): 295-302, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31306107

RESUMO

OBJECTIVES: This study was to explore the prognostic value of connective tissue growth factor (CTGF) expression in endometrial cancer (EC). METHODS: We compared CTGF expression in 198 samples from patients with endometrial cancer and 50 samples from patients with healthy endometrial tissues as determined by immunohistochemistry. RESULTS: Expression of CTGF was significantly higher in endometrial cancers as compared to normal endometrial tissues. Positive CTGF expression displayed a strong association with CA125 level, histological grade, depth of myometrial invasion and the International Federation of Gynecology and Obstetrics (FIGO) stage. Our findings revealed histological grade, depth of myometrial invasion, FIGO stage, vascular/lymphatic invasion, and the CTGF expression are related to 5-year survival in patients with endometrial cancer. Positive CTGF expression, lymph node status, as well as vascular/lymphatic invasion, were identified as independent prognostic factors in endometrial cancer. CONCLUTIONS: Over-expression of CTGF is an independent prognostic factor that will allow the successful differentiation of high-risk population from the group of patients with stage III-IV endometrial cancer. The up-regulation of CTGF may contribute to the progression of endometrial cancer and serve as a new prognostic biomarker in patients with endometrial cancer survival.


Assuntos
Fator de Crescimento do Tecido Conjuntivo/metabolismo , Neoplasias do Endométrio/genética , Adulto , Idoso , Progressão da Doença , Neoplasias do Endométrio/patologia , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Prognóstico , Adulto Jovem
20.
Artigo em Inglês | MEDLINE | ID: mdl-31354635

RESUMO

Background: Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with an unfavorable prognosis and limited treatment options. Nevertheless, no clinically applicable molecular markers have been identified for the progression of ACCs. DNA methylation alterations were found to contribute to the development of ACC in recent decades. Material and Methods: The aims of the current study was to identify the abnormally methylated differentially expressed genes (DEGs) in ACCs, and to elucidate the mechanistic basis for these changes. Analyses were conducted on gene expression and gene methylation profile datasets to identify the aberrantly methylated DEGs. The DAVID software was used to conduct the analyses of functional enrichment on screened genes. Finally, expression was validated, and the relationship between abnormally methylated DEGs and clinical features was determined via the Oncomine database and The Cancer Genome Atlas (TCGA). To further verify the altered expression and methylation status of our identified genes we also validated these changes at the tissue and cellular levels. Results: We screened and identified 92 differentially expressed genes and 802 abnormally methylated genes. Furthermore, seven aberrantly methylated and dysregulated genes were identified and validated, along with a number of functional enriched pathways. Among these seven genes, the expression or methylation status is significantly correlated with different pathological stages and overall rates of survival. In validation, the expression of seven genes were significantly altered and five genes were hypermethylated in ACC. Conclusions: Our study identified abnormally methylated DEGs and potentially affected pathways in ACCs, from which we could begin to understand the basic molecular mechanisms of these alterations. Moreover, these abnormally methylated genes might serve as therapeutic targets and biomarkers to allow ACC patients to be more precisely diagnosed and effectively treated.

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