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1.
J Appl Microbiol ; 2020 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-32427406

RESUMO

AIMS: To reveal whether the patterns of abundant and rare subcommunity composition of both bacteria and microeukaryotes vary between connected regions with different levels of nutrient loading in freshwater lakes. METHODS AND RESULTS: We investigated the abundant and rare subcommunity composition of both bacteria and microeukaryotes in two connected zones [(Meiliang Bay (MLB) and Xukou Bay (XKB)] of a large shallow freshwater Lake Taihu via the high-throughput sequencing of bacterial 16S rRNA and microeukaryotic 18S rRNA genes. Even though these two lake zones are connected and share a species bank, they diverge in community composition. Significantly higher alpha-diversity was observed for the abundant bacterial subcommunity in the MLB. However, no significant difference in alpha-diversity between the rare bacterial subcommunities, as well as both rare and abundant microeukaryotic subcommunities were observed between MLB and XKB. It is demonstrated that both environmental factors and geographic distance play central roles in controlling the rare and abundant microbial subcommunities in the two connected lake zones. CONCLUSIONS: The abundant subcommunity composition of bacteria and microeukaryotes vary between connected regions with different levels of nutrient loading. Dispersal limitation plays a vital role in shaping microbial communities even in connected zones of freshwater lakes. SIGNIFICANCE AND IMPACT OF THE STUDY: Leading to a comprehensive understanding of the characteristics of microbial community in connected lake regions with different levels of nutrient loading.

2.
J Cell Mol Med ; 2020 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-32441055

RESUMO

Long-term abuse of ketamine causes ketamine-induced cystitis. The functional alterations of bladder epithelial cells in microenvironment during cystitis remain poorly understood. Here, we explored extracellular vesicles (EV) alteration in ketamine-induced toxicity. To simulate the high-concentration ketamine environment in vivo, we established an in vitro model of high ketamine using human uroepithelial cells (SV-HUC-1). Cell viability and proliferation were assessed to evaluate the effects of various concentrations (0, 0.25, 0.5, 1, 2, 4 and 8 mmol/L) of ketamine on SV-HUC-1 cells. The cell supernatant cultured at a concentration (0, 1, 2, 4 mmol/L) of ketamine was selected for EV extraction and identified. Subsequently, we assessed different groups (ketamine, ketamine plus EV blocker, EV, EV plus extracellular vesicles blocker) of oxidative stress and expression of inflammation. Last, luciferase reporter assay was performed to study the transcriptional regulation of EV on the NF-kB and P38 pathway. The results of our study suggested that treatment with 0, 1, 2 or 4 mmol/L ketamine altered the morphology and secretion capacity of extracellular vesicles. As the concentration of ketamine increased, the average particle size of EV decreased, but the crest size, particle concentration and EV protein increased. Moreover, after the addition of EV blocker, EV secreted at different concentrations were blocked outside the cell membrane, and the degree of oxidative stress decreased. Our study provided evidence that ketamine alters the secretion of EV by directly stimulating cells in inflammation microenvironment and EV play significant roles in intercellular signal communication and the formation of KIC.EV.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32385080

RESUMO

The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bulk sediment between summer and winter. The BCCs were analyzed using the high-throughput sequencing of bacterial 16S rRNA gene, meanwhile null model analysis was employed to characterize their assembly mechanisms. The sources of the endosphere BCCs were quantitatively examined using SourceTracker from bulk sediment, rhizosphere, and seed. We observed the highest α-diversity and the lowest ß-diversity of BCCs in the rhizosphere in both seasons. We also found significant increase in α- and ß-diversity in summer than in winter among the three compartments respectively. It was demonstrated that rhizosphere sediments were the main source (∼70%) of root endosphere bacteria during both seasons. Null-model tests indicated that stochastic processes primarily affected endosphere BCCs, whereas both deterministic and stochastic processes dictated bacterial assemblages of the rhizosphere with the relative importance of stochastic versus deterministic processes depending on the season. This study suggests that multiple mechanisms of bacterial selection and community assembly exist both inside and outside of P. australis roots in different seasons.IMPORTANCE Understanding the composition and assembly mechanisms of root-associated microbial communities of plants is crucial for understanding the interactions between plants and soil. Most previous studies about plant root-associated microbiome focused on model and economic plants with less temporal or seasonal investigations. The assembly mechanisms of root-associated bacterial communities in different seasons remain poorly known, especially for the aquatic macrophytes. Here we compared the diversity, composition, and the relative importance of two different assembly processes (stochastic and deterministic processes) of bacterial communities associated with bulk, rhizosphere, and endosphere of Phragmites australis in summer and winter. While we found apparent differences in composition, diversity, and assembly processes of bacterial community among different compartments, season played important roles in determining BCCs and their diversity patterns and assemblages. We also found that endosphere bacteria are mainly originated from the rhizosphere. The obtained results add new knowledge to the plant-microbe interactions in aquatic ecosystem.

4.
Int J Stroke ; : 1747493020913557, 2020 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-32223541

RESUMO

In China, stroke is a major cause of mortality, and long-term physical and cognitive impairment. To meet this challenge, the Ministry of Health China Stroke Prevention Project Committee (CSPPC) was established in April 2011. This committee actively promotes stroke prevention and control in China. With government financial support of 838.4 million CNY, 8.352 million people from 536 screening points in 31 provinces have received stroke screening and follow-up over the last seven years (2012-2018). In 2016, the CSPPC issued a plan to establish stroke centers. To shorten the pre-hospital period, the CSPPC established a stroke center network, stroke map, and stroke "Green Channel" to create three 1-h gold rescue circles, abbreviated as "1-1-1" (onset to call time <1 h; pre-hospital transfer time < 1 h, and door-to-needle time < 1 h). From 2017 to 2018, the median door-to-needle time dropped by 4.0% (95% confidence interval (CI), 1.4-9.4) from 50 min to 48 min, and the median onset-to-needle time dropped by 2.8% (95% CI, 0.4-5.2) from 180 min to 175 min. As of 31 December 2018, the CSPPC has established 380 stroke centers in mainland China. From 1 November 2018, the CSPPC has monitored the quality of stroke care in stroke center hospitals through the China Stroke Data Center Data Reporting Platform. The CSPPC Stroke program has led to a significant improvement in stroke care. This program needs to be further promoted nationwide.

5.
Chemistry ; 2020 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-32320099

RESUMO

Covalent organic frameworks (COFs) are an emerging kind of crystalline porous polymers which present the precise integration of organic building blocks into extensible structures with regular pores and periodic skeletons. The diversity of organic units and covalent linkages makes COFs a rising materials platform for the design of structure and functionality. In this review, we summarize the recent research progress in developing COFs for photoluminescent materials. We highlight structural and functional design strategies, and identify fundamental problems that need to be solved in conjunction with potential applications from perspectives of photoluminescent materials.

6.
Int J Mol Med ; 45(5): 1341-1350, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32323735

RESUMO

Silibinin is a flavonoid extracted from milk thistle seeds which has been widely used as a hepatoprotective and antioxidant agent. Recently, accumulating evidence has demonstrated the anti­cancer effects of silibinin in various cancer models. It was previously reported that silibinin induced apoptosis and decreased metastasis by activating autophagy in renal cell carcinoma (RCC). However, the underlying molecular mechanisms by which silibinin regulates autophagy remain largely unknown. The aim of the present study was to investigate the effects of silibinin on RCC metastasis in vitro and in vivo, with a focus on autophagy­dependent Wnt/ß­catenin signaling. Human RCC 786­O and ACHN cell lines were used as the model system in vitro and RCC xenografts of nude mice were used for in vivo studies. Silibinin inhibited metastasis and epithelial­mesenchymal transition (EMT) of RCC in vitro and in vivo, by regulating the Wnt/ß­catenin signaling pathway. Furthermore, silibinin inhibited the Wnt/ß­catenin signaling pathway in an autophagy­dependent manner. Autophagic degradation of ß­catenin induced by silibinin was associated with the anti­metastatic effects of silibinin against RCC. These findings identify a novel mechanism by which silibinin inhibits EMT and metastasis of RCC, highlighting a potential novel strategy for treating metastatic RCC.

7.
Environ Pollut ; 262: 114332, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32182534

RESUMO

Dibutyl phthalate (DBP) is widely used as plasticizer and has been detected in the environment, posing a threat to animal health. However, the effects of DBP on agricultural microbiomes are not known. In this study, DBP levels in black soil were evaluated, and the impact of DBP contamination on the uptake and metabolism of sugars in microbes was assessed by glucose absorption tests, metaproteomics, metabolomics, enzyme activity assays and computational simulation analysis. The results indicated that DBP contamination accelerated glucose consumption and upregulated the expression of porins and periplasmic monosaccharide ATP-binding cassette (ABC) transporter solute-binding proteins (SBPs). DBP and its metabolic intermediates (carboxymuconate and butanol) may form a stable complex with sugar transporters and enhance the rigidity and stability of these proteins. Sugar metabolism resulting in the generation of ATP and reducing agent (NADPH), as well as the expression of some key enzymes (dehydrogenases) were also upregulated by DBP treatment. Moreover, a diverse bacterial community appears to utilize sugar, suggesting that there are widespread effects of DBP contamination on soil microbial ecosystems. The results of this study provide a theoretical basis for investigating the toxicological effects of DBP on microbes in black soil.

8.
BMC Ophthalmol ; 20(1): 107, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32183742

RESUMO

BACKGROUND: To measure the microcirculation change of macula and optic nerve head before and after femtosecond laser assisted laser in situ keratomileusis. METHODS: In total 45 eyes from 45 subjects, who underwent FS-LASIK during June 2017 to December 2017 in Guangdong Provincial People's Hospital, were recruited in this study. Vessel density in macula and optic nerve head were measured by optical coherence tomography angiography before and after transient elevation in intraocular pressure caused by application of suction ring during surgery. RESULTS: Vessel density (VD) at superficial (SCP) plexus of macular region did not differ after surgery (F(3,132) = 1.41, P = 0.24), while the deep (DCP) plexus of macular region significantly decreased 1 day after surgery (P = 0.001) but returned to its baseline value 1 month postoperatively (P = 0.1). Vessel density of optic nerve head region had no significant changes after surgery (F(2.51,95.18) = 0.6, P = 0.59). CONCLUSIONS: A short-term temporary decrease of vessel density at deep layer of macular region was observed in eyes undergoing FS-LASIK. However, the retinal capillary density went back to preoperative level 1 month after surgery. Therefore, transient IOP spike during FS-LASIK did not cause long-term decline of retinal microcirculation.

9.
Chem Biol Interact ; 320: 109022, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32112862

RESUMO

Epithelial mesenchymal transformation plays a crucial role in the metastasis of bladder cancer, which makes bladder cancer difficult to cure. Bladder cancer is the most common malignancy of the urinary system, and distant metastasis is the leading cause of death. Therefore, finding a bioactive drug that can specifically inhibit epithelial mesenchymal transformation may be a new direction for bladder cancer treatment in the future. Thymoquinone (TQ), the major active compound isolated from black seed oil (Nigella sativa), has been reported to exhibit anti-inflammatory and anticancer abilities. TQ can exhibit its antitumor effect by inhibiting the proliferation and metastasis of cancer cells. However, the underlying mechanism of TQ as a tumor inhibitor in bladder cancer remains poorly understood. First, in this research, we demonstrate that TQ can reverse EMT by upregulating epithelial markers, such as E-cadherin, and downregulating mesenchymal markers, such as N-cadherin and vimentin. Furthermore, we demonstrate that TQ can suppress the activation of the Wnt/ß-catenin signaling pathway and inhibit the expression of ß-catenin target genes, such as MYC, Axin-2, MMP7, CyclinD1 and MET, which play crucial roles in EMT and cancer progression. Additionally, we demonstrate that TQ can inhibit the growth of xenografts and restrict the formation of tumor metastatic foci in the lung. Taken together, our findings confirm the antimetastatic effect of TQ in bladder cancer cells for the first time and also provide new evidence for the development of TQ as a novel treatment for metastatic bladder cancer.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Benzoquinonas/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Proteínas Wnt/metabolismo , beta Catenina/metabolismo , Benzoquinonas/química , Linhagem Celular Tumoral , Ensaios de Migração Celular , Movimento Celular/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Estrutura Molecular , Proteínas Wnt/genética , beta Catenina/genética
10.
Anticancer Drugs ; 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32209841

RESUMO

Silibinin is a flavonoid extract isolated from milk thistle and has been proved to be a promising chemotherapeutic drug for cancer. However, most of those studies were performed on the human cancer cells, where the effects of silibinin could only be observed on an animal model with a deficient immune system. RenCa cells were isolated from a murine spontaneous renal cell carcinoma, which resembles many features of human renal cell carcinoma, and have been used to establish animal models with a sound immune response. Herein, we investigated the anti-cancer effects of silibinin on RenCa cells, revealing that it inhibited cell viability in both dose- and time-dependent manners. Silibinin slightly triggered apoptosis and significantly induced G2-M cell cycle arrest by downregulating cyclin B1 and CDK1 and increasing expression of p21. Furthermore, silibinin significantly inhibited the growth of RenCa cell xenografts in vivo. In addition, we found that silibinin reduced programmed cell death 1 ligand 1 expression of RenCa cells in vivo and in vitro. Our findings demonstrate that silibinin can inhibit the growth of mouse tumor cells in an animal model with an intact immune system, and silibinin may decrease the immunosuppression effect of tumor cells. Our results provide new evidence for evaluation of Silibinin application in cancer therapy.

11.
Funct Plant Biol ; 47(4): 342-354, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32040939

RESUMO

In China, maize-soybean relay-intercropping system follow the two main planting-patterns: (i) traditional relay-intercropping; maize-soybean equal row planting, where soybean experience severe maize shading on both sides of plants, and (ii) modern relay-intercropping; narrow-wide row planting, in this new planting pattern only one side of soybean leaves suffer from maize shading. Therefore, in this study, changes in morphological traits, cytochrome content, photosynthetic characteristics, carbon status, and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were investigated at 30 days after treatment (DAT) in shade-tolerant soybean variety Nandou-12 subjected to three different types of shading conditions; normal light (NL, all trifoliate-leaves of soybean plants were under normal light); unilateral shade (US, all right-side trifoliate-leaves of soybean plants from top to bottom were under shade while all the left-side of trifoliate-leaves from top to bottom were in normal light); bilateral shade (BS, all trifoliate-leaves of soybean plants were under complete shade). Compared with BS, US conditions decreased plant height and increased stem diameter, leaf area, and biomass at 30 DAT. Biomass distribution rates to stem, petiole and leaves, and photosynthetic characteristics were markedly improved by the US at all sampling stages, which proved to be a better growing condition than BS with respect to shade tolerance. The enhanced net photosynthesis and transpiration rates in the left-side leaves (LS) of soybean plants in US, when compared with the LS in BS, allowed them to produce higher total soluble sugar (by 70%) and total soluble protein (by 17%) at 30 DAT which reduce the adverse effects of shading at right-side leaves (RS) of the soybean plants. Similarly, soybean leaves under US accumulated higher proline content in US than the leaves of BS plants. Soybean leaves grown in shading conditions (LS and RS of BS and RS of US) developed antioxidative defence-mechanisms, including the accelerated activities of SOD, POD, APX, and CAT. Comparatively, soybean leaves in US displayed lower activity levels of the antioxidative enzymes than the leaves of BS plants, showing that soybean plants experienced less shade stress in US as compared with BS treatment. Overall, these results indicate that the association of improved photosynthetic characteristics, sugar and protein accumulation and optimum antioxidative defences could be an effective approach for growing soybean in intercropping environments.

12.
Mikrochim Acta ; 187(3): 176, 2020 02 19.
Artigo em Inglês | MEDLINE | ID: mdl-32076892

RESUMO

The authors describe a microfluidic chip-based aptasensor platform combined with magnetic tripartite DNA structure-functionalized nanocomposites to achieve simultaneous determination of kanamycin (KANA), aflatoxin M1 (AFM1), and 17ß-estradiol (E2) in milk. The two-duplex tripartite DNA nanostructure was first assembled on the surface of magnetic beads. When the aptamer on the probes recognized the specific target, the aptamer-target would be released into the supernatant. The pre-primer@circular DNA template structure initiates rolling circle amplification (RCA) by phi29 polymerase. After magnetic separation, the magnetic nanocomposites were added into a solution containing three different lengths of complementary strands to the RCA products. The number of complementary strands significantly decrease, and this can be quantitated by the microfluidic chip. Further, the employment of magnetic nanocomposites and microfluidic chip not only resolve the complex matrix interference, but also dramatically enhances the determination selectivity and sensitivity. This aptasensor allows for determination of KANA, AFM1, and E2 with limits of detection as low as 0.32 pg mL-1, 0.95 pg mL-1, and 6.8 pg mL-1, respectively. This novel method exhibits the advantages of excellent stability and fast response time (< 3 min on microfluidic chip platform) for simultaneous determination of KANA, AFM1, and E2 in milk samples and ensures food safety. Graphical abstract.

13.
Diabetes Ther ; 11(3): 701-710, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32020552

RESUMO

INTRODUCTION: The aim of this study was to compare the efficacy of vildagliptin as add-on therapy to short-term continuous subcutaneous insulin infusion (CSII) with CSII monotherapy in hospitalized patients with type 2 diabetes mellitus (T2DM). METHODS: A total of 200 hospitalized patients with inadequately controlled T2DM were randomized into groups, with one group receiving CSII monotherapy (CSII group, n =100) and the other group receiving CSII plus vildagliptin as add-on (CSII + Vig group, n = 100). Of these, 191 completed the 7-day trial (CSII group, n = 99; CSII + Vig group, n = 92) and included in the analysis. The glycemic control and variability of the patients were measured using all-day capillary blood glucose (BG) monitoring. Weight and fasting C-peptide levels were evaluated before and after the interventions. RESULTS: Mean BG concentrations during the whole treatment period were lower and the time to reach target BG was reduced in the CSII + Vig group compared with the CSII group (9.89 ± 3.37 vs. 9.46 ± 3.23 mmol/L, P < 0.01; 129 ± 4 vs. 94 ± 5 h, P < 0.01, respectively). Similarly, the indicators of glycemic variability, namely the standard deviation of BG and the largest amplitude of glycemic excursion, were significantly decreased in the CSII + Vig group compared with the CSII group (2.68 ± 1.05 vs. 2.39 ± 1.00 mmol/L, P < 0.01; 7.19 ± 2.86 vs. 6.23 ± 2.73 mmol/L, P < 0.01, respectively). CONCLUSIONS: Short-term CSII with vildagliptin as add-on therapy may be an optimized treatment for hospitalized patients with T2DM compared with short-term CSII monotherapy.

14.
Clin Exp Optom ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32052475

RESUMO

BACKGROUND: The aim of this study was to compare the macular and peripapillary vessel densities in eyes of young Chinese adults with different degrees of myopia and to evaluate the association of macular and peripapillary vessel densities with axial length and retinal nerve fibre layer thickness. METHODS: A total of 128 eyes (mild myopia, 42; moderate myopia, 45; severe myopia, 41) underwent optical coherence tomography angiography imaging. Parameters assessed were vessel densities in the superficial capillary plexus and deep capillary plexus of the macular area, peripapillary vessel density, retinal nerve fibre layer thickness, foveal thickness and foveal avascular zone area (mm2 ). RESULTS: Vessel densities in the macular and peripapillary areas as well as peripapillary retinal nerve fibre layer thickness decreased significantly when comparing high myopia to mild myopia. Axial length was significantly associated with vessel density in the macular area (superficial capillary plexus: r = -0.249, p = 0.008; deep capillary plexus: r = -0.398, p < 0.001), peripapillary area (r = -0.204, p = 0.028), foveal avascular zone area (r = -0.309, p < 0.001), and foveal thickness (r = 0.354, p < 0.001). Negative correlations were found between axial length and peripapillary vessel density as well as retinal nerve fibre layer thickness at the nasal superior, nasal inferior and inferior nasal quadrants. CONCLUSION: Varying degrees of myopia affected macular and peripapillary vessel densities as well as retinal nerve fibre layer thickness in young healthy adults. The high myopic group had the lowest vessel density in the superficial capillary plexus, deep capillary plexus of the macular area and the peripapillary area. With increased axial length, macular and peripapillary vessel densities, retinal nerve fibre layer thickness and foveal avascular zone area reduced while foveal thickness increased.

15.
Theranostics ; 10(3): 1230-1244, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31938062

RESUMO

Rationale: Glycogen synthase kinase-3ß (GSK-3ß) plays key roles in metabolism and many cellular processes. It was recently demonstrated that overexpression of GSK-3ß can confer tumor growth. However, the expression and function of GSK-3ß in hepatocellular carcinoma (HCC) remain largely unexplored. This study is aimed at investigating the role and therapeutic target value of GSK-3ß in HCC. Methods: We firstly clarified the expression of GSK-3ß in human HCC samples. Given that deviated retinoid signalling is critical for HCC development, we studied whether GSK-3ß could be involved in the regulation. Since sorafenib is currently used to treat HCC, the involvement of GSK-3ß in sorafenib treatment response was determined. Co-immunoprecipitation, GST pull down, in vitro kinase assay, luciferase reporter and chromatin immunoprecipitation were used to explore the molecular mechanism. The biological readouts were examined with MTT, flow cytometry and animal experiments. Results: We demonstrated that GSK-3ß is highly expressed in HCC and associated with shorter overall survival (OS). Overexpression of GSK-3ß confers HCC cell colony formation and xenograft tumor growth. Tumor-associated GSK-3ß is correlated with reduced expression of retinoic acid receptor-ß (RARß), which is caused by GSK-3ß-mediated phosphorylation and heterodimerization abrogation of retinoid X receptor (RXRα) with RARα on RARß promoter. Overexpression of functional GSK-3ß impairs retinoid response and represses sorafenib anti-HCC effect. Inactivation of GSK-3ß by tideglusib can potentiate 9-cis-RA enhancement of sorafenib sensitivity (tumor inhibition from 48.3% to 93.4%). Efficient induction of RARß by tideglusib/9-cis-RA is required for enhanced therapeutic outcome of sorafenib, which effect is greatly inhibited by knocking down RARß. Conclusions: Our findings demonstrate that GSK-3ß is a disruptor of retinoid signalling and a new resistant factor of sorafenib in HCC. Targeting GSK-3ß may be a promising strategy for HCC treatment in clinic.

16.
Aging (Albany NY) ; 12(2): 1128-1140, 2020 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-31951595

RESUMO

In order to explore frailty subtypes and find their associated risk factors, we conducted cross-sectional surveys of 5,341 seniors aged 60 and over in China using the Frailty Index (FI) scale. We identified four frailty subtypes, namely multi-frail, cognitive and functionally frail, psychologically frail and physiologically frail. Old age and low education level were the common risk factors among the four subtypes. Being widowed, divorced or unmarried was a risk factor for multi-frail, cognitive and functionally frail and psychologically frail, and male sex was a protective factor against cognitive and functionally frail and psychologically frail subtypes. Having a harmonious relationship with family was a protective factor against multi-frail, and fewer visits to the elderly by their children was a risk factor for psychologically frail. Dissatisfaction with their housing was a risk factor for cognitive and functionally frail, psychologically frail and physiologically frail, and a pension being the main source of income was a risk factor for cognitive and functionally frail and psychologically frail. Exercising every day was a protective factor against multi-frail and cognitive and functionally frail, and a lower level of physical activity was a risk factor for all four frailty subtypes. Our findings confirm the heterogeneity of frailty and suggest that different frail elderly individuals need more targeted care interventions.

17.
J Cardiothorac Vasc Anesth ; 34(3): 624-631, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31986286

RESUMO

OBJECTIVE: To evaluate the association between preoperative pulse pressure (PP) and the incidences of renal, neurologic, cardiac, and mortality outcomes after surgery. DESIGN: Systematic review and meta-analysis of cohort studies. SETTING: Hospitals. PARTICIPANTS: Patients who underwent cardiac or noncardiac surgeries. INTERVENTION: The preoperative PP was measured. MEASUREMENT AND MAIN RESULTS: Relevant cohort studies were obtained by systematic search of PubMed and Embase databases. A randomized effect model was used to pool the results. The multivariate adjusted risk ratio (RR) and its 95% confidence intervals (CI) were calculated to reflect the association between preoperative PP and adverse postoperative outcomes. Twelve cohort studies that included 40,143 patients who had undergone cardiac, vascular, or noncardiac surgery were included in the meta-analysis. The results showed that above a threshold of 40 mmHg, an increase in preoperative PP of 10 mmHg was independently associated with increased risk for renal events (adjusted RR: 1.13, 95% CI 1.08-1.19, p < 0.001; I2 = 0%), neurologic events (adjusted RR: 1.75, 95% CI 1.01-3.02, p = 0.04; I2 = 70%), cardiac events (adjusted RR: 1.19, 95% CI 1.03-1.37, p = 0.01; I2 = 0%), major cardiovascular adverse events (adjusted RR: 1.62, 95% CI 1.10-2.41, p = 0.02; I2 = 0%), and overall mortality (adjusted RR: 1.13, 95% CI 1.07-1.20, p < 0.001; I2 = 0%) after surgery. CONCLUSIONS: Patients with higher-than-normal preoperative PP are at increased risk for adverse postoperative outcomes.

18.
Histol Histopathol ; : 18202, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31965560

RESUMO

Chaperone-mediated autophagy (CMA), a selective form of autophagy, where cellular proteins with KFERQ-like motif are targeted to the lysosome for degradation, is necessary to maintain cellular homeostasis. The role of CMA in neurodegenerative diseases has been extensively studied in the past decades, with defects in the pathway being strongly associated with disease. Recently, accumulating evidence has demonstrated a consistent increase in basal CMA activity in a wide array of cancer cell lines and human tumor biopsies, suggesting a potential link between CMA and cancer. On the other hand, an anti-oncogenic role for CMA under physiological conditions in non-transformed cells is also proposed despite the pro-tumorigenic function of CMA in cancer cells. The growing number of connections between CMA and cancers has generated interest in modulating CMA activity for therapeutic purposes. Here, we describe recent advances in the understanding of the molecular regulation of CMA, and discuss the evidence in support of the contribution of CMA dysfunction to cancers.

19.
Oncol Rep ; 43(2): 415-426, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31894286

RESUMO

Colon cancer is one of the most common malignancies. Although there has been great development in treatment regimens over the last few decades, its prognosis remains poor. There is still a clinical need to find new drugs for colon cancer. Evodiamine (Evo) is a quinolone alkaloid extracted from the traditional herbal medicine plant Evodia rutaecarpa. In the present study, CCK­8, flow cytometry, reverse transcription quantitative polymerase chain reaction, western blot analysis and a xenograft tumor model were used to evaluate the anti­cancer activity of Evo in human colon cancer cells and determine the possible mechanism underlying this process. It was revealed that Evo exhibited prominent anti­proliferation and apoptosis­inducing effects in HCT116 cells. Bone morphogenetic protein 9 (BMP9) was notably upregulated by Evo in HCT116 cells. Exogenous BMP9 potentiated the anti­cancer activity of Evo, and BMP9 silencing reduced this effect. In addition, HIF­1α was also upregulated by Evo. The anticancer activity of Evo was enhanced by HIF­1α, but was reduced by HIF­1α silencing. BMP9 potentiated the effect of Evo on the upregulation of HIF­1α, and enhanced the antitumor effect of Evo in colon cancer, which was clearly reduced by HIF­1α silencing. In HCT116 cells, Evo increased the phosphorylation of p53, which was enhanced by BMP9 but reduced by BMP9 silencing. Furthermore, the effect of Evo on p53 was potentiated by HIF­1α and reduced by HIF­1α silencing. The present findings therefore strongly indicated that the anticancer activity of Evo may be partly mediated by BMP9 upregulation, which can activate p53 through upregulation of HIF­1α, at least in human colon cancer.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31893996

RESUMO

BACKGROUND: Prostate cancer remains one of both the most common and deadliest forms of cancer, which is still localized generally respond well to radical prostatectomy and associated interventions, up to 30% of these individuals will still suffer from disease relapse. BUB1B has been found to be essential for cell growth and proliferation, even in several kinds of tumor cells. But the specific importance and mechanistic role of BUB1B in prostate cancer remains unclear. METHODS: Quantitative Real-Time PCR and Western-blot were used in detection of mRNA and protein expression. Lentivirus infection was used to overexpression or knock down target gene. Flow cytometry analysis was performed to test protein expression and apoptosis level. Immunohistochemistry was used to identify protein expression in tissue. Statistical differences between two groups are evaluated by two-tailed t-tests. The comparison among multiple groups is performed by one-way Analysis of Variance (ANOVA) followed by Dunnett's posttest. The statistical significance of the Kaplan-Meier survival plot is determined by log-rank analysis. RESULTS: In the present report, we found BUB1B expression to be highly increased in prostate cancer tissues relative to normal controls. We further found BUB1B to be essential for efficient tumor cell proliferation, and to correlate with poorer prostate cancer patient outcomes. From a mechanistic perspective, the ability of BUB1B to regulate MELK was found to be essential for its ability to promote prostate cancer cell proliferation. CONCLUSION: Altogether, our data suggests that BUB1B is up-regulated in prostate cancer, suggesting that growth of cancer cells may depend on BUB1B-dependent regulation of MELK transcription. BUB1B may serve as a clinical prognostic factor and a druggable target for prostate cancer.

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