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Zhongguo Zhong Yao Za Zhi ; 46(12): 3116-3122, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467703


Screening suitable reference genes is the premise of quantitative Real-time PCR(qRT-PCR)for gene expression analysis. To provide stable reference genes for expression analysis of genes in Aconitum vilmorinianum, this study selected 19 candidate re-ference genes(ACT1, ACT2, ACT3, aTUB1, aTUB2, bTUB, 18S rRNA, UBQ, eIF2, eIF3, eIF4, eIF5, CYP, GAPDH1, GAPDH2, PP2A1, PP2A2, ACP, and EF1α) based on the transcriptome data of A. vilmorinianum. qRT-PCR was conducted to profile the expression of these genes in the root, stem, leaf, and flower of A. vilmorinianum. The Ct values showed that 18S rRNA with high expression level and GAPDH2 with large expression difference among organs were not suitable as the reference genes. NormFinder and geNorm showed similar results of the expression stability of the other candidate reference genes and demonstrated PP2A1, EF1α, and CYP as the highly stable ones. However, BestKeeper suggested EF1α, ACT3, and PP2A1 as the top stable genes. In view of the different results from different softwares, the geometric mean method was employed to analyze the expression stability of the candidate re-ference genes, the results of which indicated that PP2A1, EF1α, and ACT3 were the most stable. Based on the comprehensive analysis results of geNorm, NormFinder, BestKeeper, and geometric mean method, PP2A1 and EF1α presented the most stable expression in different organs of A. vilmorinianum. PP2A1 and EF1α were the superior reference genes for gene expression profiling in different organs of A. vilmorinianum.

Aconitum , Perfilação da Expressão Gênica , Genes de Plantas/genética , Reação em Cadeia da Polimerase em Tempo Real , Padrões de Referência , Reação em Cadeia da Polimerase Via Transcriptase Reversa
Biotech Histochem ; : 1-9, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33595373


During hibernation of Pelodiscus sinensis, sperm mature and are stored in the epididymis. We investigated seasonal changes in the morphology of epithelial cells of the epididymis of P. sinensis and changes in expression of cytoplasmic creatine kinase (CK). We found that the epididymal epithelium proliferates rapidly to form multiple layers from June to September, while the epididymal epithelial cells are arranged in a single layer from October to May. From the March before the mating period to the end of the mating period in September, a large amount of neutral glycoprotein is secreted in the epididymal epithelium and in the sperm aggregation area; after October, the glycoprotein in the epididymis decreases. At sperm maturation, cytoplasmic CK is expressed abundantly in the villous epithelium, which is formed by proliferation of epididymal epithelial cells. During hibernation and reproduction, the epididymal epithelium of P. sinensis exhibits different proliferation and secretion patterns as the animal adapts to two types of sperm storage. Cytoplasmic CK may participate in regulating the energy metabolism of the epididymal epithelium; it is an important enzyme for regulating sperm maturation.

Zhonghua Yan Ke Za Zhi ; 42(10): 878-82, 2006 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-17217778


OBJECTIVE: To analyze the disparity evoked potentials (DEP) of fine disparities, coarse disparities, crossed disparities and uncrossed disparities and provide parameters of impersonal examining stereopsis. METHODS: A software package for generating dynamic random dot stereogram (DRDS) was developed as a visual stimulus to elicit DEP in 30 normal subjects. The DEP of every subject was recorded in different crossed and uncrossed disparity stimuli (4', 8', 15', 23', 30', 45', 53', 60', 72', 87', 102', 124', 150' of arc). RESULTS: (1) the constant negative-positive complex wave was observed in different disparity stimuli. (2) The N wave's latency of -45' was the longest, the N wave's latency of -150' was the shortest among crossed disparities. The N wave's latency of 4', 23' was the longest, the N wave's latency of 124' was the shortest among uncrossed disparities. (3) The amplitude peak of P wave occurred at -23', -60', -150' among crossed disparities. The amplitude peak of P wave occurred at 15', 45', 72' among uncrossed disparities. (4) All characteristic crossed disparities were smaller than the uncrossed disparities. The change orderliness of small crossed disparities was different from that of small uncrossed disparities. CONCLUSIONS: The N wave's latency and the P wave's amplitude can be used as parameters to impersonally examine stereopsis. These results suggest that stereopsis can be divided into fine crossed stereopsis, fine uncrossed stereopsis, coarse crossed stereopsis, and coarse uncrossed stereopsis.

Potenciais Evocados Visuais/fisiologia , Disparidade Visual/fisiologia , Adolescente , Adulto , Diagnóstico por Computador , Feminino , Humanos , Masculino , Valores de Referência