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2.
Mol Neurobiol ; 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36441480

RESUMO

Nicotinamide phosphoribosyltransferase (NAMPT) is the key enzyme in the salvaging synthesis pathway of the nicotinamide adenine dinucleotide (NAD). Both NAMPT and NAD progressively decline upon aging and neurodegenerative diseases. The depletion of NAMPT induces mitochondrial dysfunction in motor neurons and causes bioenergetic stress in neurons. However, the roles of NAMPT in hippocampus neurons need to be further studied. Using floxed Nampt (Namptflox/flox) mice, we knocked out Nampt specifically in the hippocampus CA1 neurons by injecting rAAV-hSyn-Cre-APRE-pA. The depletion of NAMPT in hippocampus neurons induced cognitive deficiency in mice. Nevertheless, no morphological change of hippocampus neurons was observed with immunofluorescent imaging. Under the transmission electron microscope, we observed mitochondrial swollen and mitochondrial number decreasing in the cell body and the neurites of hippocampus neurons. In addition, we found the intracellular Aß (6E10) increased in the hippocampus CA1 region. The intensity of Aß42 remained unchanged, but it tended to aggregate. The GFAP level, an astrocyte marker, and the Iba1 level, a microglia marker, significantly increased in the mouse hippocampus. In the primary cultured rat neurons, NAMPT inhibition by FK866 decreased the NAD level of neurons at > 10-9 M. FK866 dropped the mitochondrial membrane potential in the cell body of neurons at > 10-9 M and in the dendrite of neurons at > 10-8 M. FK866 decreased the number and shortened the length of branches of neurons at > 10-7 M. Together, likely due to the injury of mitochondria, the decline of NAMPT level can be a critical risk factor for neurodegeneration.

3.
Artigo em Inglês | MEDLINE | ID: mdl-36404609

RESUMO

BiFeO3-BaTiO3 (BF-BT) dielectric ceramics are receiving more and more concern for advanced energy storage devices owing to their excellent ferroelectric properties and environmental sustainability. However, the energy density and efficiency are limited in spite of the large remanent polarization. Herein, we proposed a multiscale optimization strategy via a local compositional disorder with a Birich content and nanodomain engineering by introducing the Sr0.7Bi0.2Ca0.1TiO3 (SBCT) into BF-BT ceramics. Interestingly, an extraordinary energy storage property (ESP) with a high reversible energy storage density (Wrec) of ∼3.79 J/cm3 and an ultrahigh polarization discrepancy (ΔP) of ∼58.5 µC/cm2 were obtained in the SBCT-modified BF-BT ceramics under 160 kV/cm. The boosted ESP should be attributed to the fact that the replacement of A/B-sites cations could transform the long-range ferroelectric order of the BF-BT system into polar nanoregions (PNRs) along with the refined grain size, decreased leakage current, and broadened energy band gap. Moreover, good frequency (1-103 Hz) and temperature (25-125 °C) stabilities, high fatigue resistance (× 105), large power density (∼31.1 MW/cm3), and fast discharge time (∼97 ns) were also observed for the optimized ceramics. These results illustrate a potentially effective method for creating high ESP lead-free ceramics at a low electric field.

4.
Front Immunol ; 13: 980231, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36439162

RESUMO

Coronavirus disease 2019 (COVID-19), an infectious acute respiratory disease caused by a newly emerging RNA virus, is a still-growing pandemic that has caused more than 6 million deaths globally and has seriously threatened the lives and health of people across the world. Currently, several drugs have been used in the clinical treatment of COVID-19, such as small molecules, neutralizing antibodies, and monoclonal antibodies. In addition, several vaccines have been used to prevent the spread of the pandemic, such as adenovirus vector vaccines, inactivated vaccines, recombinant subunit vaccines, and nucleic acid vaccines. However, the efficacy of vaccines and the onset of adverse reactions vary among individuals. Accumulating evidence has demonstrated that circular RNAs (circRNAs) are crucial regulators of viral infections and antiviral immune responses and are heavily involved in COVID-19 pathologies. During novel coronavirus infection, circRNAs not only directly affect the transcription process and interfere with viral replication but also indirectly regulate biological processes, including virus-host receptor binding and the immune response. Consequently, understanding the expression and function of circRNAs during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection will provide novel insights into the development of circRNA-based methods. In this review, we summarize recent progress on the roles and underlying mechanisms of circRNAs that regulate the inflammatory response, viral replication, immune evasion, and cytokines induced by SARS-CoV-2 infection, and thus highlighting the diagnostic and therapeutic challenges in the treatment of COVID-19 and future research directions.


Assuntos
COVID-19 , Humanos , RNA Circular/genética , SARS-CoV-2 , Vacinas de Produtos Inativados , Replicação Viral , Vacinas Sintéticas
5.
AAPS PharmSciTech ; 23(8): 291, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36319901

RESUMO

Vaccines used for managing Newcastle disease virus (NDV) rely heavily on cold chain, and this results in major constraints in their successful application, shipping, and storage. This study was undertaken to improve the thermotolerance properties of live attenuated NDV vaccines using vacuum foam drying (VFD) technology. The live attenuated NDV vaccine formulated in 15% trehalose, 2.5% gelatin, 0.05% pluronic, and 25 mmol/L potassium phosphate buffer (T5) and dried using VFD showed improved heat tolerance in comparison to the vaccine formulated in T5 as well, but dried using freeze-drying (FD) method. The T5-formulated VFD vaccine was stored at 37°C for 120 days, 45°C for 7 days, and 60°C for 3 days; the virus titer loss decreased by no more than 1.0 Log10. In contrast, the FD vaccine prepared in T5 could only be stored at 37°C for 7-10 days. Furthermore, the T5-formulated NDV-VFD vaccine remained infectious when heated at 100°C for 30 min. Shelf-life studies confirmed the improved thermal tolerance of the T5-formulated NDV-VFD vaccine since it could be stably stored at 2-8°C for 42 months and 25°C for 15 months. Moreover, immunization of 1-month-old specific pathogen-free (SPF) chickens with the T5-formulated NDV-VFD vaccine stored at 25 and 37°C could produce hemagglutination inhibition (HI) antibody levels comparable to those of commercial NDV-FD vaccines, which require strict adherence to the cold chain. In conclusion, not only did the VFD technology improve the thermostability and long-term shelf life of the vaccine, it also maintained its immunogenicity.


Assuntos
Galinhas , Vírus da Doença de Newcastle , Animais , Vacinas Atenuadas , Vácuo , Organismos Livres de Patógenos Específicos
6.
Mediators Inflamm ; 2022: 1061658, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36211987

RESUMO

Background: Functional nasal endoscopic surgery (FESS) is an effective treatment approach for chronic rhinosinusitis with nasal polyps (CRSwNP) patients, but some patients still suffer from postoperative recurrence. This study is aimed at investigating the expression of multiple cytokines in CRSwNP and revealing their relationships with postoperative recurrence. Methods: A total of 72 patients with CRSwNP, including 36 primary and 36 recurrent patients, were enrolled. Serum samples were obtained, 30 cytokine levels were measured by multiplex analysis, and the association between cytokine levels and recurrence was assessed. The most potential cytokines were further validated in another independent cohort with 60 primary and 60 recurrent CRSwNP patients. Results: The results of multiple cytokine profiling exhibited that the levels of eotaxin, G-CSF, IFN-α, IL-13, IL-17A, IL-5, MCP-1, and RANTES were vastly changed in the recurrent group in comparison with the primary group. Receiver-operating characteristic (ROC) curves highlighted that serum levels of eotaxin, IL-17A, and RANTES were strongly predictive of postoperative recurrence (area under the curve (AUC) > 0.7, P < 0.05). Further validation results showed that elevated serum eotaxin, IL-17A, and RANTES levels were enhanced in the recurrent group. The ROC curve showed that serum eotaxin (AUC = 0.729, P < 0.001) and RANTES (AUC = 0.776, P < 0.001) exhibited stronger ability than serum IL-17A (AUC = 0.617, P = 0.027) in predicting CRSwNP recurrence. Conclusion: Our data suggested that serum multiple cytokine profiling was associated with postoperative recurrence of CRSwNP, and eotaxin and RANTES might serve as potential biomarkers for predicting postoperative recurrence. These results might contribute to the understanding of the underlying mechanisms of recurrence and provide novel clues for precision therapy in CRSwNP.


Assuntos
Pólipos Nasais , Rinite , Sinusite , Biomarcadores/metabolismo , Quimiocina CCL5 , Doença Crônica , Citocinas/metabolismo , Fator Estimulador de Colônias de Granulócitos , Humanos , Interleucina-13 , Interleucina-17 , Interleucina-5 , Pólipos Nasais/metabolismo , Pólipos Nasais/cirurgia , Rinite/metabolismo , Rinite/cirurgia , Sinusite/metabolismo , Sinusite/cirurgia
8.
Nano Lett ; 22(18): 7667-7673, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36083833

RESUMO

Two-dimensional semiconducting transition metal dichalcogenides (TMDs) enable ultimate channel length scaling of transistor technology due to their atomic-thin body nature, which also brings the challenge of a pronounced self-heating effect inside the ultrathin channel. In particular, high current density under high electric field could lead to negative differential resistance behavior due to self-heating, not only limiting the current carrying capability of the TMDs transistors but also leading to severe reliability issues. Here, we report high-performance monolayer WS2 transistors on a high-thermal-conductivity BeO dielectric with effective suppression of the self-heating effects, eliminating the negative differential resistance behavior at high field, as observed in the case of the HfO2 dielectric. The monolayer CVD WS2 device on BeO with a 50 nm channel length exhibits a record-high on-state current of 325 µA/µm, transconductance (gm) of 150 µS/µm, and a on/off ratio of 1.8 × 108 at Vds = 1 V, far exceeding previous results.

9.
Trials ; 23(1): 816, 2022 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-36167574

RESUMO

BACKGROUND: High-grade glioma (HGG) is the most malignant brain tumor with poor outcomes. Whether anesthetic methods have an impact on the outcome of these patients is still unknown. Retrospective study has found no difference between intravenous and inhalation anesthesia on the overall survival (OS) of the HGG patients, however, intravenous anesthesia with propofol might be beneficial in a subgroup of patients with a Karnofsky Performance Status (KPS) Scale less than 80. Further prospective studies are needed to evaluate the results. METHODS: This is a single-centered, randomized controlled, parallel-group trial. Three hundred forty-four patients with primary HGG for tumor resection will be randomly assigned to receive either intravenous anesthesia with propofol or inhalation anesthesia with sevoflurane. The primary outcome is the OS of the patients within 18 months. Secondary outcomes include progression-free survival (PFS), the numerical rating scale (NRS) of pain intensity and sleep quality, the postoperative encephaloedema volume, complications, and the length of hospital stay of the patients. DISCUSSION: This is a randomized controlled trial to compare the effect of intravenous and inhalation anesthesia maintenance on the outcome of supratentorial HGG patients. The results will contribute to optimizing the anesthesia methods in these patients. TRIAL REGISTRATION: ClinicalTrials.gov NCT02756312. Registered on 29 April 2016 and last updated on 9 Sep 2020.


Assuntos
Anestésicos Inalatórios , Glioma , Propofol , Anestesia por Inalação/efeitos adversos , Anestésicos Intravenosos/efeitos adversos , Glioma/cirurgia , Humanos , Ensaios Clínicos Pragmáticos como Assunto , Propofol/efeitos adversos , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Retrospectivos , Sevoflurano
10.
Anal Chim Acta ; 1221: 340110, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35934352

RESUMO

A novel sulfate-loaded iron-nitrogen co-doped carbon quantum dots (SO42--CQDs)-based fluorescent method was synthesized by the facile and environmentally friendly pyrolysis of persimmon frost (carbon source) and (NH4)2Fe(SO4)2·6H2O. After SMMC-7721 cells were incubated with the SO42--CQDs for 24 h, more than 95% of the cells remained viable, even at a high concentration of the SO42--CQDs, indicating excellent biocompatibility and low toxicity. In addition, it was able to be taken up by the cells to emit their bright blue fluorescence after excitation at 365 nm, indicating suitable cell permeability. The SO42--CQDs also exhibited a unique response to changes in pH, which was applied in the detection of OPs by relying on the production of acetic acid from the hydrolysis of acetylcholine (ACh) by acetylcholinesterase (AChE), which decreased the pH and engendered an increase in the fluorescence of the SO42--CQDs; however, the inhibition of AChE by glyphosate resulted in little influence on fluorescence intensity due to the lack of acetic acid produced. This mechanism was the basis for the development of a sensitive assay for the detection of glyphosate. The resulting assay had a limit of detection of 0.066 ng/mL. Furthermore, the method was successfully applied for the precise and accurate monitoring of the concentration, distribution, and variation of glyphosate residues in chives and cultivated soil. Therefore, the proposed method was anticipated to provide a promising alternative for other detection methods to enable the reliable analysis of OPs in food products.


Assuntos
Pontos Quânticos , Acetilcolinesterase , Carbono/química , Corantes Fluorescentes/química , Glicina/análogos & derivados , Concentração de Íons de Hidrogênio , Nitrogênio/química , Pontos Quânticos/química
11.
Inorg Chem ; 61(36): 14275-14281, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36031796

RESUMO

Rational selection of metal ions and organic ligands to synthesize metal-organic complexes (MOCs) is necessary for constructing multifunctional materials. Herein, we have obtained a novel heterotrimetallic Zn2Dy2Ir pentanuclear MOC by the assembly of DyIII, luminescent ZnII(valpn), and [IrIII(H2L)(ppy)2]Cl metalloligands (Hppy = 2-phenylpyridine, H2L = 2,2'-bipyridine-5,5'-di-p-benzoic acid). Single-crystal structural analysis shows that the central [IrIII(L)(ppy)2]- bridges two ZnDy moieties using two carboxylates of L2-. Measurements of organic light-emitting diodes (OLEDs) show that the maximum luminance is 284.2 cd/m2 and the turn-on voltage is 6 V. Magnetic studies reveal that Zn2Dy2Ir is a field-induced single-molecule magnet (SMM) with an energy barrier of 19.1(2) K under a 2 kOe dc field. Zn2Dy2Ir shows luminescence sensing with a quenching efficiency of up to 99.0% for 2,4,6-trinitrophenol (TNP).

12.
Exp Ther Med ; 24(3): 568, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35978928

RESUMO

Preeclampsia (PE), presenting with onset hypertension and proteinuria, is a pregnancy-specific disorder that can result in maternal and fetal morbidity and mortality. Insufficient trophoblast invasion and migration has been considered to be an important cause of this disease. The present study aimed to investigate the role of peptidyl arginine deiminase 4 (PAD4), whose knockdown has been previously indicated to reduce inflammation and susceptibility to pregnancy loss in mice, in the development of PE in vitro. Lipopolysaccharide (LPS) was used to treat a human trophoblast cell line (HTR8/SVneo). After PAD4 silencing via transfection with short hairpin RNA against PAD4, the concentrations of inflammatory factors IL-6, IL-12 and monocyte chemoattractant protein (MCP)-1 were measured using ELISA. Cell viability was also measured using Cell Counting Kit-8 assay. HTR8/SVneo cell invasion and migration were detected using Transwell and wound healing assays, respectively. Western blotting was used to measure the expression of citrullinated NF-κB essential modulator (NEMO) and nuclear NF-κB p65 protein levels. TNF-α was applied for evaluating the potential regulatory effects of PAD4 on NF-κB in LPS-stimulated HTR8/SVneo cells. LPS increased the levels of IL-6, IL-12 and MCP-1 and reduced the migration and invasion of HTR8/SVneo cells. PAD4-knockdown was found to markedly reduce the levels of IL-6, IL-12 and MCP-1 secretion. HTR8/SVneo cell invasion and migration was also significantly elevated after PAD4 silencing following LPS exposure. In addition, LPS stimulation notably upregulated the protein levels of citrullinated NEMO and nuclear NF-κB p65, which was restored by PAD4 knockdown. Furthermore, TNF-α treatment partially counteracted the effects of PAD4 knockdown on the secretion of IL-6, MCP-1 and IL-12, which are markers of inflammation, and invasion and migration in LPS-induced HTR8/SVneo cells. To conclude, these results suggest that PAD4 silencing can suppress inflammation whilst promoting invasion and migration by trophoblast cells through inhibiting the NEMO/NF-κB pathway. These findings furthered the understanding in the complex molecular mechanism that can trigger PE and provide a promising target for the treatment of this disease.

13.
J Zhejiang Univ Sci B ; 23(7): 607-612, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35794690

RESUMO

Neutrophils are predominant leukocytes in the circulation, which are essential for killing invading pathogens via the activation of effector responses and the production of reactive oxygen species (ROS), also named as "oxidative burst." When infected, activated neutrophils fight bacteria, fungi, and viruses through oxidative burst, phagocytosis, degranulation, and the production of neutrophil extracellular traps (NETs) in a neutrophil death process named as "NETosis" (Mutua and Gershwin, 2021). NETs, consisting of DNA fibers decorated with modified histones and numerous antimicrobial proteins from cytoplasmic granules and the nucleus, can either be beneficial or detrimental (Mutua and Gershwin, 2021). Several pathways can lead to this death process. In response to various stimuli, NETosis traps and clears pathogens, facilitating phagocytosis by other neutrophils and phagocytes. However, excessive NETosis often results in disease due to increasing the pro-inflammatory response and perpetuating the inflammatory condition (Hellebrekers et al., 2018; Hidalgo et al., 2019; Klopf et al., 2021). Accordingly, inhibiting aberrant NETosis may alleviate the severity of various autoimmune and inflammatory diseases.


Assuntos
Armadilhas Extracelulares , Explosão Respiratória , DNA , Armadilhas Extracelulares/metabolismo , Neutrófilos/metabolismo , Espécies Reativas de Oxigênio/metabolismo
14.
Methods ; 205: 97-105, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35781051

RESUMO

The International Classification of Diseases (ICD), which is endorsed by the World Health Organization, is a diagnostic classification standard. ICD codes store, retrieve, and analyze health information to make clinical decisions. Currently, ICD coding has been adopted by more than 137 countries. However, in Pakistan, very few hospitals have implemented ICD coding and conducted different epidemiological studies. Moreover, none of them have reported the spectrum of liver disease burden based on ICD coding, nor implemented automated ICD coding. In this study, we annotated ICD codes for the database of the liver transplant unit of the Pir Abdul Qadir Shah Jeelani Institute of Medical Sciences. We named this database Medical Information Mart for Liver Transplantation (MIMLT). The results revealed that the database contains 34 ICD codes, of which V70.8 is the most frequent code. Furthermore, we determined the spectrum of liver disease burden in liver recipients based on ICD coding. We found that chronic hepatitis C (070.54) is the most frequent indication for liver transplantation. Additionally, we implemented automated ICD coding utilizing the MIMLT database and proposed a novel Deep Recurrent Convolutional Neural Network with Transfer Learning through pre-trained Embeddings (DRCNNTLe) model, which is an extended version of our DRCNN-HP model. DRCNNTLe extracts robust text representations from its pre-trained embedding layer, which is trained on a large domain-specific MIMIC III database corpus. The results indicate that utilizing pre-trained word embeddings, which are trained on large domain-specific corpora can significantly improve the performance of the DRCNNTLe model and provide state-of-the-art results when the target database is small.


Assuntos
Codificação Clínica , Registros Eletrônicos de Saúde , Classificação Internacional de Doenças , Aprendizado de Máquina , Redes Neurais de Computação
15.
Artigo em Inglês | MEDLINE | ID: mdl-35805486

RESUMO

Groundwater is a significant component of water resources, but drinking groundwater with excessive heavy metals (HMs) is harmful to human health. Currently, quantitative source apportionment and probabilistic health risk assessment of HMs in groundwater are relatively limited. In this study, 60 groundwater samples containing seven HMs were collected from Hainan Island and analyzed by the coupled absolute principal component scores/multiple linear regression (APCS/MLR), the health risk assessment (HRA) and the Monte Carlo simulation (MCS) to quantify the pollution sources of HMs and the health risks. The results show that the high-pollution-value areas of HMs are mainly located in the industry-oriented western region, but the pollution level by HMs in the groundwater in the study area is generally low. The main sources of HMs in the groundwater are found to be the mixed sources of agricultural activities and traffic emissions (39.16%), industrial activities (25.57%) and natural sources (35.27%). Although the non-carcinogenic risks for adults and children are negligible, the carcinogenic risks are at a high level. Through analyzing the relationship between HMs, pollution sources, and health risks, natural sources contribute the most to the health risks, and Cr is determined as the priority control HM. This study emphasizes the importance of quantitative evaluation of the HM pollution sources and probabilistic health risk assessment, which provides an essential basis for water pollution prevention and control in Hainan Island.


Assuntos
Água Subterrânea , Metais Pesados , Poluentes do Solo , Adulto , Criança , China , Monitoramento Ambiental , Humanos , Modelos Lineares , Metais Pesados/análise , Método de Monte Carlo , Medição de Risco , Poluentes do Solo/análise
16.
Brief Bioinform ; 23(5)2022 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-35849093

RESUMO

The coronavirus disease 2019 pandemic has alerted people of the threat caused by viruses. Vaccine is the most effective way to prevent the disease from spreading. The interaction between antibodies and antigens will clear the infectious organisms from the host. Identifying B-cell epitopes is critical in vaccine design, development of disease diagnostics and antibody production. However, traditional experimental methods to determine epitopes are time-consuming and expensive, and the predictive performance using the existing in silico methods is not satisfactory. This paper develops a general framework to predict variable-length linear B-cell epitopes specific for human-adapted viruses with machine learning approaches based on Protvec representation of peptides and physicochemical properties of amino acids. QR decomposition is incorporated during the embedding process that enables our models to handle variable-length sequences. Experimental results on large immune epitope datasets validate that our proposed model's performance is superior to the state-of-the-art methods in terms of AUROC (0.827) and AUPR (0.831) on the testing set. Moreover, sequence analysis also provides the results of the viral category for the corresponding predicted epitopes with high precision. Therefore, this framework is shown to reliably identify linear B-cell epitopes of human-adapted viruses given protein sequences and could provide assistance for potential future pandemics and epidemics.


Assuntos
COVID-19 , Vírus , Aminoácidos , Mapeamento de Epitopos/métodos , Epitopos de Linfócito B , Humanos , Aprendizado de Máquina , Peptídeos/química
17.
IEEE J Biomed Health Inform ; 26(10): 5201-5212, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-35867367

RESUMO

Automatic International Classification of Diseases (ICD) coding is defined as a kind of text multi-label classification problem, which is difficult because the number of labels is very large and the distribution of labels is unbalanced. The label-wise attention mechanism is widely used in automatic ICD coding because it can assign weights to every word in full Electronic Medical Records (EMR) for different ICD codes. However, the label-wise attention mechanism is redundant and costly in computing. In this paper, we propose a pseudo label-wise attention mechanism to tackle the problem. Instead of computing different attention modes for different ICD codes, the pseudo label-wise attention mechanism automatically merges similar ICD codes and computes only one attention mode for the similar ICD codes, which greatly compresses the number of attention modes and improves the predicted accuracy. In addition, we apply a more convenient and effective way to obtain the ICD vectors, and thus our model can predict new ICD codes by calculating the similarities between EMR vectors and ICD vectors. Our model demonstrates effectiveness in extensive computational experiments. On the public MIMIC-III dataset and private Xiangya dataset, our model achieves the best performance on micro F1 (0.583 and 0.806), micro AUC (0.986 and 0.994), P@8 (0.756 and 0.413), and costs much smaller GPU memory (about 26.1% of the models with label-wise attention). Furthermore, we verify the ability of our model in predicting new ICD codes. The interpretablility analysis and case study show the effectiveness and reliability of the patterns obtained by the pseudo label-wise attention mechanism.


Assuntos
Registros Eletrônicos de Saúde , Classificação Internacional de Doenças , Codificação Clínica , Humanos , Reprodutibilidade dos Testes
18.
Huan Jing Ke Xue ; 43(7): 3789-3798, 2022 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-35791562

RESUMO

Comprehending the distribution pattern and enrichment rule of the heavy metal cadmium (Cd) in soil and grasping its activity and influencing factors is crucial for guaranteeing the soil environment safety of agricultural and construction land and improving the overall quality of the soil environment. The concentration of heavy metal Cd in the quaternary profile of Nansha was measured, and the pollution level was evaluated using the geographical accumulation index (Igeo) and potential ecological risk index (Er). Then, we determined the correlation between the total amount of Cd and the physicochemical properties of the soil using factor analysis (FA-MLR). The results showed that the average content of Cd in shallow soil (0-20 cm) in the Nansha area was 0.54 mg·kg-1, and the soil was mainly weakly acidic and neutral. The average content of Cd in deep soil (150-200 cm) was 0.42 mg·kg-1, and the soil was mainly neutral. At the same time, the variation coefficient of Cd content in shallow soil was significantly greater than that in deep soil, and Cd content tended to be consistent with the increase in depth. The Cd content of quaternary sediments showed a bimodal distribution with depth, reaching a peak at 20-25 m and 5-10 m, respectively, whereas the core sediments in this section were mostly marine sediments of silt and silty clay, with high cation exchange capacity and organic matter. The Igeo and Er of Cd in the fine-grained sediments dominated by clayey soil were significantly higher than those in the coarse-grained sediments dominated by sandy soil. The difference in soil pH value led to the different trend of Cd content with depth; the migration amount and vertical migration depth of Cd in deep soil were greater in the acidic environment. The influence of pH change on the migration and transformation of Cd was not completely reflected in the influence on the total amount of Cd, but more importantly, it changed the effective state of Cd, thus affecting the bioavailability of Cd. The unstable Cd composed of water-soluble, ion-exchangeable, and carbonate states accounted for 37.92%-49.10% of the total Cd, resulting in a strong instability of soil Cd. Further investigation on the migration and toxicity of heavy metals associated with human health risks is needed in future studies.


Assuntos
Metais Pesados , Poluentes do Solo , Disponibilidade Biológica , Cádmio/análise , China , Monitoramento Ambiental/métodos , Humanos , Metais Pesados/análise , Solo/química , Poluentes do Solo/análise
19.
Front Genet ; 13: 798020, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35664322

RESUMO

Esophageal cancer (ESCA) is one of the common malignant tumors. The roles and signaling mechanisms of spindle apparatus coiled-coil protein 1 (SPDL1) in ESCA progression have not been reported previously. Therefore, the expression levels and potential clinical roles of SPDL1 were investigated using data from multiple databases and tissue samples of 53 ESCA patients who underwent 18F-FDG positron emission tomography (PET)/computed tomography (CT) before therapy. The signaling mechanisms of SPDL1 involved in ESCA progression were investigated via bioinformatics analysis. The effects of SPDL1 on the growth and migration of ESCA cells were investigated using CCK-8, Edu, and transwell assays. SPDL1 was upregulated in ESCA tissues. Increased SPDL1 expression was associated with age, grade, drinking history, cancer stage, lymph node metastasis, TP53 mutation, and poor prognosis in patients with ESCA. SPDL1 overexpression was significantly correlated with SUVmax, SUVmean, and TLG of PET/CT. SPDL1 silencing inhibited cell proliferation, migration, and invasion. SPDL1 was significantly enriched in cell cycle, spliceosome, DNA replication, and other processes. The hub genes of a constructed protein-protein interaction network included CDK1, BUB1, CCNB1, BUB1B, CCNA2, CDC20, MAD2L1, AURKB, NDC80, and PLK1, which were related to SPDL1 expression. The findings of this study suggest that SPDL1 may serve as a biomarker of ESCA prognosis.

20.
Front Pharmacol ; 13: 871100, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35721201

RESUMO

Thyroid hormone/thyroid hormone receptor (TH/TR) axis is characterized by TH with the assistance of plasma membrane transporters to combine with TR and mediate biological activities. Growing evidence suggests that TH/TR participates in plenty of hepatic metabolism. Thus, this review focuses on the role of the TH/TR axis in the liver diseases. To be specific, the TH/TR axis may improve metabolic-associated fatty liver disease, hepatitis, liver fibrosis, and liver injury while exacerbating the progression of acute liver failure and alcoholic liver disease. Also, the TH/TR axis has paradoxical roles in hepatocellular carcinoma. The TH/TR axis may be a prospecting target to cure hepatic diseases.

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