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1.
Front Big Data ; 5: 879389, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36111178

RESUMO

Human Activity Recognition (HAR) is a prominent application in mobile computing and Internet of Things (IoT) that aims to detect human activities based on multimodal sensor signals generated as a result of diverse body movements. Human physical activities are typically composed of simple actions (such as "arm up", "arm down", "arm curl", etc.), referred to as semantic features. Such abstract semantic features, in contrast to high-level activities ("walking", "sitting", etc.) and low-level signals (raw sensor readings), can be developed manually to assist activity recognition. Although effective, this manual approach relies heavily on human domain expertise and is not scalable. In this paper, we address this limitation by proposing a machine learning method, SemNet, based on deep belief networks. SemNet automatically constructs semantic features representative of the axial bodily movements. Experimental results show that SemNet outperforms baseline approaches and is capable of learning features that highly correlate with manually defined semantic attributes. Furthermore, our experiments using a different model, namely deep convolutional LSTM, on household activities illustrate the broader applicability of semantic attribute interpretation to diverse deep neural network approaches. These empirical results not only demonstrate that such a deep learning technique is semantically meaningful and superior to its handcrafted counterpart, but also provides a better understanding of the deep learning methods that are used for Human Activity Recognition.

2.
J Chem Phys ; 157(10): 104501, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36109218

RESUMO

A new glass forming method is essential for broadening the scope of liquid and glassy metal-organic frameworks due to the limitations of the conventional melt-quenching method. Herein, we show that in situ mechanical vibration can facilitate the framework melting at a lower temperature and produce glassy metal-organic frameworks (MOFs) with unique properties. Using zeolitic imidazolate framework (ZIF)-62 as a concept-proofing material, in situ mechanical vibration enables low-temperature melting at 653 K, far below its melting point (713 K). The resultant vibrated ZIF-62 glass exhibited a lower glass transition temperature of 545 K, improved gas accessible porosity, and pronounced short-to-medium range structures compared to the corresponding melt-quenched glass. We propose that vibration-facilitated surface reconstruction facilitates pre-melting, which could be the cause of the lowered melting temperature. The vibration assisted method represents a new general method to produce MOF glasses without thermal decomposition.

3.
Chemosphere ; 308(Pt 1): 136307, 2022 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-36067812

RESUMO

Anaerobic ammonium oxidation (anammox) is carbon friendly biological nitrogen removal process, and recently more focus is given to improving the anammox activity. Because of its high adsorption and modifiability, graphene and its derivative in wastewater treatment have received much attention. However, the specific effects and mechanisms of graphene oxide (GO) and reduced graphene oxide (RGO) on anammox are still controversial. Extensive data analysis was performed to explore the effects of GO and RGO on anammox. Statistical analysis revealed that 100 mg/L GO significantly promoted the anammox process, while 200 mg/L of GO inhibited the anammox process. The promotion of anammox performance under the influence of RGO was dependent on the temperature. The Logistic model was utilized for depicting the variation of nitrogen removal efficiency under promoting dosage of graphene oxides. A neural network model-based analysis was performed to reach anammox's potential mechanisms under the influence of two graphene oxides. Spearman correlation analysis showed that GO and RGO had significant positive correlations with nitrogen removal efficiency and specific anammox activity (p < 0.01), especially for RGO. In addition, the abundance of Planctomycetes and Nitrospirae was positively correlated with the addition of graphene oxides. This work comprehensively unraveled the role of graphene oxide materials on the anammox process and provided practical directions for the enhancement of anammox.

4.
Bioresour Technol ; 363: 127901, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36075349

RESUMO

Sulfur autotrophic denitrifiers and heterotrophic denitrifiers widely exist in aquatic ecosystem, however, the response of sulfide to the microbial community structure in mixotrophic denitrification ecosystem is unknown yet. In this study, the denitrification performance and microbial community were explored by changing the molar ratio of influent C/N/S. From the level of genus, the joint action of Thauera, Pacacoccus, Fusibacter Pseudoxanthomonas, Thiobacillus, Sulfurovum and Sulfurimonas brought about the efficient denitrification performance in the mixotrophic system. Thauera increased from from 0.97% to more than 13%, and the relative abundances of Thiobacillus and Sulfurimonas were about 4.14% and 3.89% separately after adding S2-. The results of this study showed that the denitrification performance could be indeed intensified in the mixotrophic system, among which provided a theoretical basis for establishing an efficient biological nitrogen removal system.

5.
Gynecol Endocrinol ; : 1-7, 2022 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-36151730

RESUMO

AIMS: To investigate the regulatory mechanism of SCF expression in human GCs of PCOS related follicles. MATERIALS AND METHODS: SCF, BMP15 and HIF-1α were evaluated in human serums, follicular fluids (FFs) and GCs, which were collected from 69 PCOS patients and 74 normal ovulatory patients. KGN cell line was used in this study. RESULTS: Our results showed that the rate of MII oocyte and 2PN fertilization was lower in PCOS group, though PCOS patients retrieved much more oocytes. The level of BMP15 in FF and the level of SCF in serum and FF were also lower in PCOS patients. We found a weakened expression of HIF-1α and SCF in GCs from PCOS patients when compared with the non-PCOS patients. The expression of HIF-1α and SCF was significantly increased in KGN cells after treating cells with rhBMP15, however, this promotion effects of BMP15 on HIF-1α and SCF expression were obviously abolished by co-treatment with BMP-I receptor inhibitor (DM). Moreover, knock down of HIF-1α expression in KGN cells significantly reduced the expression of SCF in human GCs, in spite of activating BMP15 signaling pathway. CONCLUSIONS: The present study suggest that BMP15 could induce SCF expression by up-regulating HIF-1α expression in human GCs, the aberrance of this signaling pathway might be involved in the PCOS related abnormal follicular development.

6.
Plant Cell Environ ; 2022 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-35982608

RESUMO

Arbuscular mycorrhizal (AM) symbiosis modulates plant-herbivore interactions. Still, how it shapes the overall plant defence strategy and the mechanisms involved remain unclear. We investigated how AM symbiosis simultaneously modulates plant resistance and tolerance to a shoot herbivore, and explored the underlying mechanisms. Bioassays with Medicago truncatula plants were used to study the effect of the AM fungus Rhizophagus irregularis on plant resistance and tolerance to Spodoptera exigua herbivory. By performing molecular and chemical analyses, we assessed the impact of AM symbiosis on herbivore-triggered phosphate (Pi)- and jasmonate (JA)-related responses. Upon herbivory, AM symbiosis led to an increased leaf Pi content by boosting the mycorrhizal Pi-uptake pathway. This enhanced both plant tolerance and herbivore performance. AM symbiosis counteracted the herbivore-triggered JA burst, reducing plant resistance. To disentangle the role of the mycorrhizal Pi-uptake pathway in the plant's response to herbivory, we used the mutant line ha1-2, impaired in the H+ -ATPase gene HA1, which is essential for Pi-uptake via the mycorrhizal pathway. We found that mycorrhiza-triggered enhancement of herbivore performance was compromised in ha1-2 plants. AM symbiosis thus affects the defence pattern of M. truncatula by altering resistance and tolerance simultaneously. We propose that the mycorrhizal Pi-uptake pathway is involved in the modulation of the plant defence strategy.

7.
BMC Pediatr ; 22(1): 486, 2022 08 13.
Artigo em Inglês | MEDLINE | ID: mdl-35964110

RESUMO

BACKGROUND: Coffin-Siris syndrome (CSS) is a rare autosomal dominant disorder characterized by intellectual disability, developmental delay, and characteristic facial features. Few patients with cutaneous phenotype in this rare syndrome have been reported. CASE PRESENTATION: Herein, we describe a 12-year-old Chinese girl diagnosed with CSS, who was referred to our hospital because of intellectual disability and short stature. Prominent characteristics of the cutaneous system were observed: (1) A congenital giant nevus from the left frontal and temporal regions to the entire left scalp; and (2) multiple melanocytic nevi on the face and trunk. Whole exome sequencing revealed a novel heterozygous variant in the ARID1B gene. Recombinant human growth hormone (rhGH) was given for short stature, and resulted in significantly improved height. No enlargement or malignant transformation of nevi occurred within 4 years of follow-up. CONCLUSION: The symptoms in cutaneous system is noteworthy,which may be a neglected phenotype in CSS.The therapeutic response of growth hormone is effective in this patient and no tumor related signs were found.


Assuntos
Nanismo , Deformidades Congênitas da Mão , Micrognatismo , Nevo Pigmentado , Anormalidades Múltiplas , Criança , Proteínas de Ligação a DNA/genética , Nanismo/genética , Face/anormalidades , Feminino , Deformidades Congênitas da Mão/diagnóstico , Deformidades Congênitas da Mão/genética , Humanos , Deficiência Intelectual , Micrognatismo/genética , Pescoço/anormalidades , Nevo Pigmentado/genética , Fatores de Transcrição/genética
8.
Foot Ankle Surg ; 2022 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-35999112

RESUMO

OBJECTIVE: To compare the mid-term clinical results of lag screw and Kirschner wire fixation(KWF) for close reduction in triplane distal tibia epiphyseal fracture. METHODS: A retrospective analysis of 25 cases of triplane fractures of the distal tibia treated in our department from Jan 2017 to Dec 2019 was performed, Lag screw fixation(LSF) was used in 14 cases and Kirschner wire fixation in 11 cases, the clinical results were evaluated by premature epiphyseal closure(PPC) rate, the American Orthopaedic Foot and Ankle Score (AOFAS) Ankle-hindfoot foot scoring system, the lateral distal tibial angle (LDTA) from X-ray. RESULTS: All the 25 children were followed up for a mean of 34(ranging 26-52) months. AOFAS scores improved from a mean of 33(ranging 29-43) pre-op, to 82(ranging 77-88) at three month follow up, to 92 (ranging 88-98) at last follow-up in all 25 cases. Till last follow up there was no cases premature physeal closure in LSF group but 4 cases in KWF group, LDTA in both groups at last follow up shows no ankle varus or valgus deformity, and the ankle joint function was not limited in all cases. CONCLUSION: Lag screw and Kirschner wire fixation methods can both achieve good clinical effects for triplane distal tibia epiphyseal fracture. Lag screw fixation provide lower PPC rate but Kirschner wire fixation save one anesthesia and surgery.

9.
Chemistry ; : e202201821, 2022 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-35869408

RESUMO

We present the construction of a M2 L4 -type metal-organic nanocage featuring four endohedral Feringa's motor motifs and its adaptive encapsulation towards a C60 guest molecule. The structure of the cage, though complicated on the 1 H NMR spectrum due to the adoption of mixed ligands, was unambiguously characterized with a combination of ESI-MS, 2D DOSY, 13 C NMR and particularly the SAXS technique. The molecular motor within the cage demonstrated similar photophysical properties to the uncoordinated one, indicating the motor's function was not compromised when it was anchored in such a confined nanospace. Furthermore, the nanocage showed good guest encapsulation ability towards C60 , and a guest induced-fit behavior of the cage was revealed based on the extensive SAXS analysis and molecular dynamics simulation. The adaptive motorized nanocage reported here represents one of the very few examples of integrating individual motors into a discrete nanoconfined system and offers prospects to achieve its non-equilibrium functions.

10.
J Assist Reprod Genet ; 39(8): 1901-1908, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35834089

RESUMO

PURPOSE: Oocyte death is a severe clinical phenotype that causes female infertility and recurrent in vitro fertilization and intracytoplasmic sperm injection failure. We aimed to identify pathogenic variants in a female infertility patient with oocyte death phenotype. METHODS: Sanger sequencing was performed to screen PANX1 variants in the affected patient. Western blot analysis was used to check the effect of the variant on PANX1 glycosylation pattern in vitro. RESULTS: We identified a novel PANX1 variant (NM_015368.4 c.86G > A, (p. Arg29Gln)) associated with the phenotype of oocyte death in a non-consanguineous family. This variant displayed an autosomal dominant inheritance pattern with reduced penetrance. Western blot analysis confirmed that the missense mutation of PANX1 (c.86G > A) altered the glycosylation pattern in HeLa cells. Moreover, the mutation effects on the function of PANX1 were weaker than recently reported variants. CONCLUSION: Our findings expand the inheritance pattern of PANX1 variants to an autosomal dominant mode with reduced penetrance and enrich the variational spectrum of PANX1. These results help us to better understand the genetic basis of female infertility with oocyte death.


Assuntos
Infertilidade Feminina , Conexinas/genética , Feminino , Células HeLa , Heterozigoto , Humanos , Infertilidade Feminina/patologia , Masculino , Proteínas do Tecido Nervoso/genética , Oócitos/patologia , Sêmen
11.
Artigo em Inglês | MEDLINE | ID: mdl-35886165

RESUMO

Improving treatment efficiency and reducing investment and operating costs make aerobic granular sludge technology (AGS) a promising technology for treating aquaculture wastewater. The development of continuous flow reactors (CFRs) has become a new direction in the research of AGS. This study clarifies the granulation effect, hydrodynamic behavior and particle separation of three different CFRs (R1 to R3). The established CFD model was able to explain the hydrodynamic behavior in all three CFRs; in particular, R3 performed the best from the perspective of hydrodynamic behavior due to its abundant turbulence. In addition, the optimal baffle distance and baffle angle of R3 were simulated to be 40 mm and 60°, respectively, due to them providing the best turbulent flow and particle separation effect. However, an overlarge baffle angle could weaken the turbulent pattern in the reactor. The retention time distribution further confirmed the reasonability of these optimal parameters with the highest effective volume ratio of 0.82. In short, this study gives an instruction for exploring the rapid formation mechanism of AGS in a CFR to promote its engineering application.


Assuntos
Esgotos , Eliminação de Resíduos Líquidos , Aerobiose , Reatores Biológicos , Hidrodinâmica
12.
Ecotoxicol Environ Saf ; 242: 113914, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35878501

RESUMO

Silicosis is a systemic disease characterized by diffuse fibrosis of the lung tissue caused by long-term inhalation of large amounts of free silica (SiO2) dust. The pathogenesis of silicosis has not been fully elucidated, and there is a lack of effective treatment methods. N-acetylcysteine (NAC) can potentially treat pulmonary fibrosis by exerting antioxidant effects. Desipramine (DMI) can influence pulmonary fibrosis development by inhibiting acid sphingomyelinase (ASMase) activity and regulating ceramide concentrations. Both can interfere with pulmonary fibrosis through different mechanisms, but the intervention effects of NAC combined with DMI on silicosis fibrosis have not been reported. Therefore, this study established a rat silicosis model using a single tracheal drip of SiO2 dust suspension in Wistar rats to investigate the effect of NAC combined with DMI on SiO2 dust-induced silicosis and its related molecular mechanisms. The histopathological examination of the SiO2 dust-induced silicosis rats suggested that NAC and DMI alone or in combination could decrease the severity of pulmonary fibrosis in rats. The combined intervention had a better effect on reducing fibrosis than the individual interventions. NAC and DMI, alone or in combination, decreased the levels of markers related to pulmonary fibrosis in rats (smooth muscle α-actin (α-SMA), collagen (Col) I, Col III, hydroxyproline (HYP), inflammatory factors (transforming growth factor-ß1 (TGF-ß1) and tumor necrosis factor-α (TNF-α)), and lipid peroxidase malondialdehyde (MDA)). The nuclear factor-erythroid 2-related factor 2 (Nrf2)/heme-oxygenase-1 (HO-1) and ASMase/ceramide pathways were inhibited to some extent by increasing the superoxide dismutase (SOD) levels of antioxidant enzymes and 8-iso-prostaglandin F2α (8-iso-PGF2α) levels of lipid peroxides. The combined intervention and NAC alone inhibited the SiO2 dust-induced elevation of matrix metalloproteinase 1 (MMP-1) and tissue inhibitor matrix metalloproteinase 1 (TIMP-1), but the effect was not significant in the DMI-treated group. Combining DMI and NAC inhibited Col I deposition and reduced HO-1, TIMP-1, and ASMase levels in lung tissues compared to individual treatments. In summary, the SiO2 dust could induce oxidative stress and inflammation in rats, resulting in an imbalance in extracellular matrix (ECM) synthesis/catabolism and ASMase/ceramide signaling pathway activation, leading to silicosis development.The combined intervention of DMI and NAC may synergistically regulate the Nrf2/HO-1 pathway, maintain the anabolic balance of the ECM, inhibit ASMase/ceramide signaling pathway activation by suppressing the inflammatory response and effectively delay silicosis fibrosis progression.


Assuntos
Acetilcisteína , Desipramina , Fibrose Pulmonar , Silicose , Acetilcisteína/metabolismo , Acetilcisteína/farmacologia , Acetilcisteína/uso terapêutico , Animais , Antioxidantes/metabolismo , Ceramidas/metabolismo , Desipramina/metabolismo , Desipramina/uso terapêutico , Modelos Animais de Doenças , Quimioterapia Combinada , Poeira , Fibrose , Heme Oxigenase (Desciclizante) , Pulmão , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 1 da Matriz/toxicidade , Fator 2 Relacionado a NF-E2 , Fibrose Pulmonar/induzido quimicamente , Fibrose Pulmonar/tratamento farmacológico , Fibrose Pulmonar/metabolismo , Ratos , Ratos Wistar , Transdução de Sinais , Dióxido de Silício/toxicidade , Silicose/metabolismo , Esfingomielina Fosfodiesterase/metabolismo , Esfingomielina Fosfodiesterase/toxicidade , Inibidor Tecidual de Metaloproteinase-1
13.
Exp Ther Med ; 24(2): 498, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35837067

RESUMO

Endometriosis is a common female gynecological disease that is characterized by the presence of functional endometrial tissue outside the uterine cavity. At present, many animal models have been established. However, previous studies consistently use human endometrial tissue implanted in the subcutaneous or abdominal cavity for modeling and rarely use endometrial cells. In the present study, we ascertained whether immortalized stromal and/or epithelial endometrial cells are able to induce subcutaneous endometriosis in nude mice. Mixed human immortalized endometriosis stromal and epithelial cells, but not the cells of Group 1 or Group 2, were successfully constructed and led to endometriotic-like lesions. The endometriosis-like lesions observed in nude mice consisted of endometriosis-like glands lined with columnar epithelial cells and surrounded by stromal cells in the fibrous fatty connective tissue. Immunofluorescence analysis showed that glandular epithelial cells were intensely stained for E-cadherin and cytokeratin 7, and surrounding stromal cells were mildly stained for neprilysin (CD10) and vimentin. Moreover, the cells present in the endometriosis-like lesions were of human origin. Our data indicate that the mixture of human immortalized endometriosis stromal cells and epithelial cells is able to establish subcutaneous endometriosis lesions in nude mice. This model could be used to understand the molecular mechanisms involved in the occurrence and development of endometriosis.

14.
Medicine (Baltimore) ; 101(28): e29794, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35839016

RESUMO

This study aimed to assess the surface electromyogram (sEMG) signal characteristics of the muscle around the hip joint after Pemberton osteotomy in children with unilateral developmental dysplasia of the hip (DDH). A total of 21 children with unilateral DDH who had received Pemberton osteotomy were selected as the DDH group, and 21 healthy children of the same age were selected as the control group. The children in both groups were tested using sEMG, the Root mean square (RMS) values of the tensor fascia lata, rectus femurs, and medial head of the hamstring and gluteus maximum on both sides in standing and walking status were recorded. The value on the affected side in the DDH group was compared with the value on the healthy side himself and the value in the control group. The mean postoperative follow-up in the DDH group was 27.76 ± 24.30 months. The RMS value of the affected gluteus maximum muscle in the DDH group was significantly larger while standing (P < 0.05), the RMS value of bilateral tensor fascia lata muscle was significantly larger while walking (P < 0.05), and the RMS value of the affected hamstring muscle medial head was significantly less in the DDH group compared with the control group (P < 0.05). An asymmetry and compensatory increase in the sEMG activity of the muscles around the hip joint when standing and walking was noted in children with unilateral DDH who underwent Pemberton osteotomy combined with a femoral osteotomy. The rehabilitation training of the muscles around the hip joint after unilateral DDH should be strengthened.


Assuntos
Displasia do Desenvolvimento do Quadril , Luxação Congênita de Quadril , Criança , Eletromiografia , Fêmur/cirurgia , Luxação Congênita de Quadril/cirurgia , Humanos , Osteotomia , Estudos Retrospectivos , Resultado do Tratamento
15.
Sci Rep ; 12(1): 13129, 2022 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-35908081

RESUMO

To address the increasing labor cost of resin tapping, more efficient methods for resin tapping need to be developed. This study aimed to evaluate the features of resinosis as affected by stimulant pastes in Pinus elliottii × P. caribaea, which is also one of the predominant resin-producing species hybrids in South China. The resin yields and resin compositions were assessed in 33 P. elliottii × P. caribaea F1 families, with the application of four kinds of chemical stimulants, potassium (K2SO4) paste, naphthalene acetic acid (NAA) paste, benzoic acid (BA) paste and 2-chloroethylphosphonic acid (CEPA) paste. Our results showed that all four pastes significantly increased the resin yield by at least 20% at each tapping, and 3- to fivefold increases were detected at the beginning of each year. The correlations between resin yield and growth at each tapping ranged from uncorrelated to moderately positively correlated, indicating that resin yield was mostly but not always determined by tree size. The concentration of each resin component did not change with the stimulant applications. In P. elliottii × P. caribaea, selecting a larger tree diameter at breast height and employing the chemical stimulants at the first several tapping rounds are efficient tapping procedures. Moreover, the K2SO4-based stimulant can be recommended considering its promoting effects on resin yield and the low cost of the chemicals required to produce it.

16.
Front Genet ; 13: 872920, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35651948

RESUMO

Background: Heart rate variability (HRV), reflecting circadian rhythm of heart rate, is reported to be associated with clinical outcomes in stage 5 chronic kidney disease (CKD5) patients. Whether CKD related factors combined with HRV can improve the predictive ability for their death remains uncertain. Here we evaluated the prognosis value of nomogram model based on HRV and clinical risk factors for all-cause mortality in CKD5 patients. Methods: CKD5 patients were enrolled from multicenter between 2011 and 2019 in China. HRV parameters based on 24-h Holter and clinical risk factors associated with all-cause mortality were analyzed by multivariate Cox regression. The relationships between HRV and all-cause mortality were displayed by restricted cubic spline graphs. The predictive ability of nomogram model based on clinical risk factors and HRV were evaluated for survival rate. Results: CKD5 patients included survival subgroup (n = 155) and all-cause mortality subgroup (n = 45), with the median follow-up time of 48 months. Logarithm of standard deviation of all sinus R-R intervals (lnSDNN) (4.40 ± 0.39 vs. 4.32 ± 0.42; p = 0.007) and logarithm of standard deviation of average NN intervals for each 5 min (lnSDANN) (4.27 ± 0.41 vs. 4.17 ± 0.41; p = 0.008) were significantly higher in survival subgroup than all-cause mortality subgroup. On the basis of multivariate Cox regression analysis, the lnSDNN (HR = 0.35, 95%CI: 0.17-0.73, p = 0.01) and lnSDANN (HR = 0.36, 95% CI: 0.17-0.77, p = 0.01) were associated with all-cause mortality, their relationships were negative linear. Spearman's correlation analysis showed that lnSDNN and lnSDANN were highly correlated, so we chose lnSDNN, sex, age, BMI, diabetic mellitus (DM), ß-receptor blocker, blood glucose, phosphorus and ln intact parathyroid hormone (iPTH) levels to build the nomogram model. The area under the curve (AUC) values based on lnSDNN nomogram model for predicting 3-year and 5-year survival rates were 79.44% and 81.27%, respectively. Conclusion: In CKD5 patients decreased SDNN and SDANN measured by HRV were related with their all-cause mortality, meanwhile, SDNN and SDANN were highly correlated. Nomogram model integrated SDNN and clinical risk factors are promising for evaluating their prognosis.

17.
RSC Adv ; 12(25): 15980-15985, 2022 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-35733682

RESUMO

Nitroxyl (HNO) has been identified as an important signaling molecule in biological systems, and it plays critical roles in many physiological processes. However, its detection remains challenging because of the limited sensitivity and/or specificity of existing detection methods. Low-frequency electron paramagnetic resonance (EPR) spectroscopy and imaging, coupled with the use of exogenous paramagnetic probes, have been indispensable techniques for the in vivo measurement of various physiological parameters owing to their specificity, noninvasiveness and good depth of magnetic field penetration in animal tissues. However, the in vivo detection of HNO levels by EPR spectroscopy and imaging is limited due to the need for improved probes. We report the first "turn on-response" EPR probe for HNO utilizing a Cu(ii) coordination-containing TAM radical (denoted as CuII[TD1]). Upon reaction with HNO, CuII[TD1] shows a 16.1-fold turn-on in EPR signal with a low detection limit of 1.95 µM. Moreover, low-temperature EPR spectroscopic and ESI-MS studies showed that the sensing mechanism relies on the reduction of Cu(ii) by HNO. Lastly, CuII[TD1] is selective for HNO over other reactive nitrogen and oxygen species except for some reductants (Cys and Asc). This new Cu(ii) coordination-containing TAM radical shows great potential for in vivo EPR HNO applications in the absence of reducing agents and provides insights into developing improved and targeted EPR HNO probes for biomedical applications.

18.
Exp Cell Res ; 417(2): 113225, 2022 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-35644414

RESUMO

Malignancies including ovarian cancer (OvCa) are genetically unstable. Genomic integrity is maintained by tumor suppressor p53 and DNA damage response network, which crosstalk to each other via not well characterized mechanisms. In this work, we characterize features of damage-related signals in cultured epithelial OvCa cells and tumor biopsies. We found that endogenous burden of DNA damage in OvCa tissues were ubiquitously accumulated in high-grade malignancies than lower grade of cancer that cannot be obviously explained by disturbed function of in DNA damage response (DDR). In contrast, CHK1 phosphorylation (CHK1-pS345) marking the checkpoint activation in nucleolar compartments are prevalent in high-grade OvCa, coincident to the elevated DNA damage in nucleoplasm. Generation of CHK1-pS345 requires the presence of p53 protein in addition to the well-known activities of ATM/ATR kinases. Apparently, mutant forms of p53 possess higher activity in triggering CHK1 phosphorylation than wild type, implying a potential role of p53 in maintaining rDNA integrity. Loss of p53 function would cause replication stress in nucleoli. Altogether, our study reveals endogenous nucleoli stress in OvCa that is coupled to perturbed function of p53 in DNA repair.


Assuntos
Neoplasias Ovarianas , Proteína Supressora de Tumor p53 , Proteínas Mutadas de Ataxia Telangiectasia/genética , Proteínas Mutadas de Ataxia Telangiectasia/metabolismo , Carcinoma Epitelial do Ovário/genética , Proteínas de Ciclo Celular/genética , Linhagem Celular Tumoral , Quinase 1 do Ponto de Checagem/genética , Quinase 1 do Ponto de Checagem/metabolismo , Dano ao DNA/genética , Feminino , Humanos , Neoplasias Ovarianas/genética , Fosforilação , Proteínas Quinases/metabolismo , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
19.
Phys Chem Chem Phys ; 24(24): 15091-15102, 2022 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-35699069

RESUMO

Different surface planes of various types of titanium dioxide (TiO2) crystals have diverse catalysis effects on the splitting of H2O and H2 and the electronic structures of the formed hydroxylated TiO2 vary significantly. A series of sixteen types of hydroxylated TiO2 surfaces containing two types of hydroxyls (OH1 and OH2) on four kinds of low index surfaces [(001), (100), (101), and (110)] of two types of crystals [anatase (A) and rutile (R)] are studied using first-principles density functional theory calculations. The catalyzed splitting of H2O and H2 on the eight low index surfaces is compared using Gibbs free energy. The geometries and electronic structures including the total and partial density of states and the charge density distribution of the sixteen hydroxylated surfaces are systematically described. The electronic structures of R-101, R-001, A-110, A-100, and A-001 surfaces are more significantly influenced by hydroxylation than other surfaces and the effects of OH2 are larger than those of OH1. In particular, the band gap values decrease and a new electronic energy state appears in R-001-OH2 and A-100-OH2. A new electronic state appears in the middle of the bands of R-101 and A-110 surfaces upon hydroxylation. The electron spin balance at the edge of the conduction band minimum of A-001-OH2 is disturbed. This research can provide theoretical guidance for experimental researchers to design surface hydroxylated TiO2 materials with tunable electronic structures and high catalytic performance.

20.
J Am Chem Soc ; 144(29): 13021-13025, 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35748600

RESUMO

Metal-organic framework (MOF) glasses are a fascinating new class of materials, yet their prosperity has been impeded by the scarcity of known examples and limited vitrification methods. In the work described in this report, we applied synergistic stimuli of vapor hydration and thermal dehydration to introduce structural disorders in interpenetrated dia-net MOF, which facilitate the formation of stable super-cooled liquid and quenched glass. The material after stimulus has a glass transition temperature (Tg) of 560 K, far below the decomposition temperature of 695 K. When heated, the perturbed MOF enters a super-cooled liquid phase that is stable for a long period of time (>104 s), across a broad temperature range (26 K), and has a large fragility index of 83. Quenching the super-cooled liquid gives rise to porous MOF glass with maintained framework connectivity, confirmed by EXAFS and PDF analysis. This method provides a fundamentally new route to obtain glassy materials from MOFs that cannot be melted without causing decomposition.

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