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1.
J Colloid Interface Sci ; 613: 136-145, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35033760

RESUMO

A multifunctional graphitic carbon nitride (GCN) protective layer with bionic ion channels and high stability is prepared to inhibit dendrite growth and side reactions on zinc (Zn) metal anodes. The high electronegativity of the nitrogen-containing organic groups (NOGs) in the GCN layer can effectively promote the dissociation of solvated Zn2+ and its rapid transportation in bionic ion channels via a hopping mechanism. In addition, this GCN layer exhibits excellent mechanical strength to suppress the growth of Zn dendrites and the volume expansion of Zn metal anodes during the plating process. Consequently, the electrodeposited Zn presents a uniform and densely packed morphology with negligible side-product accumulation. As a result, the half-cell composed of the Cu-GCN anode can deliver a remarkable long-term cycling performance of 1000 h at 0.5 mA cm-2 and 0.25 mAh cm-2. A full cell assembled with MnO2 cathode also displays improved long-term cycling performance (150 cycles at 200 mA g-1) when the Cu-GCN@Zn composite anode is applied. This work deepens our understanding of the kinetics of ion migration in the interface layer and paves the way for next-generation high energy-density Zn-metal batteries (ZMBs).

2.
Small ; : e2106707, 2022 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-35032095

RESUMO

Lithium-oxygen batteries (LOBs) with ultra-high theoretical energy density (≈3500 Wh kg-1 ) are considered as the most promising energy storage systems. However, the sluggish kinetics during the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) can induce large voltage hysteresis, inferior roundtrip efficiency and unsatisfactory cyclic stability. Herein, hydrangea-like NiO@Ni2 P heterogeneous microspheres are elaborately designed as high-efficiency oxygen electrodes for LOBs. Benefitting from the interfacial electron redistribution on NiO@Ni2 P heterostructure, the electronic structure can be modulated to ameliorate the chemisorption of the intermediates, which is confirmed by density functional theory (DFT) calculations and experimental characterizations. In addition, the interpenetration of the PO bond at the NiO@Ni2 P heterointerface leads to the internal doping effect, thereby boosting electron transfer to further improve ORR and OER activities. As a result, the NiO@Ni2 P electrode shows a low overpotential of only 0.69 V, high specific capacity of 18254.1 mA h g-1 and superior long-term cycling stability of over 1400 h. The exploration of novel bifunctional electrocatalyst in this work provides a new solution for the practical application of LOBs.

3.
Bioresour Technol ; : 126470, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34863846

RESUMO

The present study introduced a new method for enhanced biomethane production and pollution control of swine wastewater (SW) using anaerobic membrane bioreactor (AnMBR). Results confirmed 35°C as the optimum temperature for enhanced anaerobic digestion which resulted in relatively higher methane production rate and potential. In AnMBR system, robust pollutants removal and conversion rate were achieved under various hydraulic retention time (HRT) ranging from 20 to 10 days, while the highest methane yield (0.24 L/g-CODremoved) and microbial activity (6.65 mg-COD/g-VSS·h) were recorded at HRT of 15 days. Reduction of HRT to 10 days resulted in serious membrane fouling due to accumulation of extracellularpolymericsubstances(EPS) and cake layer on the membrane. However, cake layer as the dominant membrane foulant could be effectively removed through periodic physical backwash to recover the membrane permeability. Overall, the suggested AnMBR is a promising technology to enhance SW treatment and energy recovery.

4.
J Pharm Biomed Anal ; 209: 114522, 2021 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-34891006

RESUMO

In this study, ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) and ultra-high performance liquid chromatography coupled with photo-diode array spectrometry (UPLC-DAD) were firstly integrated to qualitatively characterize the chemical profiles and quantitatively determine the major components in Impatiens Caulis (IC), a Chinese medicinal herb derived from the dried stem of Impatiens balsamina. The qualitative analysis revealed that there was no difference in chemical profiles of twenty commercial IC samples. A total of 45 components were characterized, there in 5 naphthoquinones, 3 coumarins, 5 phenolic acids, 12 flavonoids and 3 other compounds were identified definitely. However, the quantitative results showed a significant difference in these IC samples. In particular, the highest content of total quantified compounds was even 9.49 times of the lowest one. Furthermore, the average contents of these quantified compounds in twenty commercial IC samples had high values of relative standard deviation (RSD) ranged from 15.64% to 98.76%, suggesting a poor quality consistency in these commercial IC samples. Therefore, efficacy related chemical markers as well as the cultivation region, harvest time and/or post-harvest handling of IC should be further investigated for ensuring its quality and efficacy consistency.

5.
J Med Chem ; 64(24): 18175-18192, 2021 12 23.
Artigo em Inglês | MEDLINE | ID: mdl-34905371

RESUMO

Human dihydroorotate dehydrogenase (hDHODH), as the fourth and rate-limiting enzyme of the de novo pyrimidine synthesis pathway, is regarded as an attractive target for malignancy therapy. In the present study, a novel series of teriflunomide derivatives were designed, synthesized, and evaluated as hDHODH inhibitors. 13t was the optimal compound with promising enzymatic activity (IC50 = 16.0 nM), potent antiproliferative activity against human lymphoma Raji cells (IC50 = 7.7 nM), and excellent aqueous solubility (20.1 mg/mL). Mechanistically, 13t directly inhibited hDHODH and induced cell cycle S-phase arrest in Raji cells. The acute toxicity assay indicated a favorable safety profile of 13t. Notably, 13t displayed significant tumor growth inhibition activity with a tumor growth inhibition (TGI) rate of 81.4% at 30 mg/kg in a Raji xenograft model. Together, 13t is a promising inhibitor of hDHODH and a preclinical candidate for antitumor therapy, especially for lymphoma.

6.
Front Pharmacol ; 12: 746208, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34912216

RESUMO

The kidneys are a pair of important organs that excretes endogenous waste and exogenous biological agents from the body. Numerous transporters are involved in the excretion process. The levels of these transporters could affect the pharmacokinetics of many drugs, such as organic anion drugs, organic cationic drugs, and peptide drugs. Eleven drug transporters in the kidney (OAT1, OAT3, OATP4C1, OCT2, MDR1, BCRP, MATE1, MATE2-K, OAT4, MRP2, and MRP4) have become necessary research items in the development of innovative drugs. However, the levels of these transporters vary between different species, sex-genders, ages, and disease statuses, which may lead to different pharmacokinetics of drugs. Here, we review the differences of the important transports in the mentioned conditions, in order to help clinicians to improve clinical prescriptions for patients. To predict drug-drug interactions (DDIs) caused by renal drug transporters, the molecular docking method is used for rapid screening of substrates or inhibitors of the drug transporters. Here, we review a large number of natural products that represent potential substrates and/or inhibitors of transporters by the molecular docking method.

7.
Sci Total Environ ; : 151350, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34728200

RESUMO

Bacterioplankton communities in rivers are strongly influenced by the surrounding landscape, yet the relationships between land use and bacterioplankton communities at multi-spatial scales and the mechanisms that shape bacterioplankton communities remain unclear. Here, we collected surface water samples from 14 tributaries of the Yuan River, a secondary tributary of the Yangtze River, which has been heavily impacted by human activities. We characterized the bacterioplankton communities by high-throughput sequencing techniques, and managed to identify the mechanisms governing bacterioplankton community assembly. The results showed that, in general, both landscape compositions and landscape configurations had significant effects on bacterial communities, and the effects were greater at the buffer scale than at the sub-basin scale. Additionally, there was no distinct distance-decay pattern for the effects of landscape structures on bacterial communities from the near-distance (100 m) to the long-distance (1000 m) buffer zones, with the maximal effects occurring in the 1000 m circular buffer (wet season) and 500 m riparian buffer (dry season) zone, respectively. Land use influenced the bacterioplankton community both directly through exogenous inputs (mass effect) and indirectly by affecting water chemistry (species sorting). Variance partitioning analyses showed that the total explanations of bacterial community variations by water chemistry and the intersections of water chemistry and land use (56.2% in wet season and 50.4% in dry season) were higher than that of land use alone (6.1% in wet season and 25.4% in dry season). These suggest that mass effects and species sorting jointly shaped bacterial community assembly, but that the effects of species sorting outweighed those of mass effects. Nevertheless, more biotic and abiotic factors need to be considered to better understand the microbial assembly mechanisms in anthropogenically influenced riverine ecosystems.

8.
Sheng Wu Gong Cheng Xue Bao ; 37(11): 3828-3835, 2021 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-34841788

RESUMO

Psoriasis is considered as an inflammatory disease driven by T cells, and its pathogenesis is closely related to the imbalance of intestinal bacteria flora. It has been reported that Bacteroides fragilis could play an anti-inflammatory role by regulating the expression of cytokines in T cells. To date, there is no report using B. fragilis to treat psoriasis. In this study, we explored the therapeutic effect of B. fragilis BF839 on psoriasis. We selected 27 psoriasis patients who were treated in the Second Affiliated Hospital of Guangzhou Medical University from April to October 2019. The patients were given B. fragilis BF839 orally for 12 weeks while maintaining the original treatment. The psoriasis area and severity index (PASI) score was evaluated before and after the treatment. The rate of drug withdrawal and reduction after 12 weeks of treatment were calculated. Our results showed that the rate of 12-week trial completion was 96.3% (26/27). We used PASIN to define the proportion of people whose PASI score decreased more than or equal to N% after treatment. At 12 weeks, PASI30, PASI50, and PASI75 were 65.4%, 42.3%, and 19.2%, respectively. The PASI score was 9.1±5.9 and 5.8±4.9 before and after 12 weeks of treatment respectively, and the difference was statistically significant (P<0.01). The effective rate of the visual analog scale (VAS) score was 42.3% at 12 weeks, and the VAS score was 2.9±2.2 and 2.3±2.1 before and after 12 weeks of treatment, respectively, which had no statistically significant difference (P>0.05). The adverse reaction rate of patients was 3.8% (1/26) within 12 weeks of treatment, including 1 case of constipation, and the rate of drug withdrawal and reduction was 60.0%. The above results suggest that B. fragilis BF839 may be functional on the treatment of psoriasis by reducing the PASI score and the drug usage rate with few side effect, which deserves further study.


Assuntos
Bacteroides fragilis , Psoríase , Anti-Inflamatórios , Citocinas , Humanos , Psoríase/tratamento farmacológico , Índice de Gravidade de Doença , Resultado do Tratamento
9.
Chem Commun (Camb) ; 57(94): 12687-12690, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34781327

RESUMO

Adverse dendritic growth destabilizes Li metal anodes (LMAs), dramatically limiting the commercial applications of Li metal batteries (LMBs). Herein, ZIF-67 with unsaturated coordinative metal sites is used to construct a protective coating to immobilize anions, which is capable of increasing the Li+ transference number (tLi+) to mitigate the electrolyte concentration gradient in the vicinity of LMAs. In addition, the ZIF-67-based layer provides highly ordered ionic diffusion pathways, thus enabling dendrite-free Li deposition. With a considerable Li+ transference number of 0.57, ZIF-67-Cu@Li symmetric cells deliver a superior long-term performance (over 1000 h), and Li-O2 full batteries with ZIF-67-Cu@Li electrodes display a high energy density of 1911.61 W h kg-1.

10.
Opt Lett ; 46(21): 5441-5444, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34724496

RESUMO

In this Letter, a metasurface combined with emerging 3D printing technology is proposed. The proposed metasurface regards the simple cube as the unit cell, and the height of the cube is the only variable. A nearly linear transmission phase range covering 360° operating at 20 GHz is obtained when the height is regulated in [2.26 mm, 11.20 mm]. Therefore, the proposed unit cell can be adopted to any metasurface with various functions. Taking the generation of a non-diffractive Bessel beam as an example, two metasurfaces composed of 30×30 units with different focusing directions are designed based on non-diffractive theory and the generalized law of refraction. Two prototypes are 3D printed and measured by a near-field scanning system. The measured results validate our design with satisfactory focusing and beam deflection performance. Additionally, the 3D printed metasurface has lower cost and a shorter processing cycle, and avoids metal loss. Therefore, a 3D printed metasurface is an excellent candidate that can be applied in millimeter wave or even higher frequency bands.

11.
Clin Transl Med ; 11(10): e541, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34709765

RESUMO

More and more evidence show that major depressive disorder (MDD) is closely related to inflammation caused by chronic stress, which seriously affects human physical and mental health. However, the inflammatory mechanism of depression and its effect on brain function have not been clarified. Based on resting-state functional magnetic resonance imaging (rs-fMRI), we investigated change of brain functional imaging and the inflammatory mechanism of damage-related molecular patterns (DAMPs)-receptor of advanced glycation protein end product (RAGE) in MDD patients and depressive-like cynomolgus monkeys and mice models induced by chronic stress. The regional homogeneity (ReHo) and functional connectivity (FC) were analyzed using MATLAB and SPM12 software. We detected the expression of DAMPs-RAGE pathway-related proteins and mRNA in MDD peripheral blood and in serum and brain tissue of cynomolgus monkeys and mice. Meanwhile, RAGE gene knockout mice, RAGE inhibitor, and overexpression of AVV9RAGE adeno-associated virus were used to verify that RAGE is a reliable potential biomarker of depression. The results showed that the ReHo value of prefrontal cortex (PFC) in MDD patients and depressive-like cynomolgus monkeys was decreased. Then, the PFC was used as a seed point, the FC of ipsilateral and contralateral PFC were weakened in depressive-like mice. At the same time, qPCR showed that RAGE and HMGB1 mRNA were upregulated and S100ß mRNA was downregulated. The expression of RAGE-related inflammatory protein in PFC of depressive-like monkeys and mice were consistent with that in peripheral blood of MDD patients. Moreover, the results were confirmed in RAGE-/- mice, injection of FPS-ZM1, and overexpression of AAV9RAGE in mice. To sum up, our findings enhance the evidence that chronic stress-PFC-RAGE are associated with depression. These results attempt to establish the links between brain functional imaging, and molecular targets among different species will help to reveal the pathophysiological mechanism of depression from multiple perspectives.

12.
Small ; : e2104349, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34713590

RESUMO

Cationic defect engineering is an effective strategy to optimize the electronic structure of active sites and boost the oxygen electrode reactions in lithium-oxygen batteries (LOBs). Herein, Ni-Fe layered double oxides enriched with cationic nickel vacancies (Ni-Fe LDO-VNi ) are first designed and studied as the electrocatalysts for LOBs. Based on the density functional theory calculation, the existence of nickel vacancy in Ni-Fe LDO-VNi significantly improves its intrinsic affinity toward intermediates, thereby fundamentally optimizing the formation and decomposition pathway of Li2 O2 . In addition, the number of eg electrons on each nickel site is 1.19 for Ni-Fe LDO-VNi , which is much closer to 1 than 1.49 for Ni-Fe LDO. The near-unity occupation of eg orbital enhances the covalency of transition metal-oxygen bonds and thus improves the electrocatalytic activity of Ni-Fe LDO-VNi toward oxygen electrode reactions. The experimental results show that the LOBs with Ni-Fe LDO-VNi electrode deliver low overpotentials of 0.11/0.29 V during the oxygen reduction reaction/oxygen evolution reaction, respectively, large specific capacities of 13 933 mA h g-1 and superior cycling stability of over 826 h. This study provides a novel approach to optimize the electrocatalytic activity of LDO through reasonable defect engineering.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34697710

RESUMO

Exposure to metal(loid)s is associated with adverse effects on human health, especially for children and adolescents. This study was designed to evaluate metal(loid)s exposure in 2050 children and adolescents aged 6-18 years from Liuzhou City, Southwest China. The detection rates of 21 elements were all above 99%. We found that age was an important predictor for most elements, and that children exhibited more exposure than adolescents, expect for strontium (p < 0.05). Interestingly, urinary levels were higher in girls for 13 of our study elements. Multiple regression models also showed that dietary habits also affected the distribution of elements. Moreover, we estimated exposure risk by generating the hazard quotient (HQ) for single metal and the hazard index (HI) for the co-occurrence of metals. The HQ of cadmium was > 1 at the P95 value and that the risk of the mixed effect of cadmium, mercury, and thallium was not negligible, and indicated that the associated risk was of concern. Our results provide basic data on the reference values of urinary metal(loid) levels and an assessment of health risks for children and adolescents that reside in industrial areas.

14.
Artigo em Inglês | MEDLINE | ID: mdl-34569645

RESUMO

BACKGROUND: TYK2 deficiency is a rare primary immunodeficiency disease caused by loss-of-function mutations of TYK2 gene, which is initially proposed as a subset of hyper-IgE syndrome (HIES). However, accumulating evidence suggests TYK2-deficient patients do not necessarily present with HIES characteristics, indicating a vacuum of knowledge on the exact roles of TYK2 in human immune system. METHOD: Pathogenic effects of patients were confirmed by qRT-PCR, Western blot, and protein stability assays. The responses to cytokines including IFN-α/ß/γ, IL-6, IL-10, IL-12, and IL-23 of peripheral blood mononuclear cells (PBMCs) from these patients were detected by Western blot, qRT-PCR, and flow cytometry. The differentiation of T and B cells was detected by flow cytometry. RESULTS: We described five more TYK2-deficient cases presenting with or without hyper-IgE levels, atopy, and distinct pathogen infection profile, which are caused by novel TYK2 mutations. These mutations were all found by high-throughput sequencing and confirmed by Sanger sequencing. The patients showed heterogeneous responses to various cytokine treatments, including IFN-α/ß/γ, IL-6, IL-10, IL-12, and IL-23. The homeostasis of lymphocytes is also disrupted. CONCLUSION: Based on our findings, we propose that TYK2 works as a multi-tasker in orchestrating various cytokine signaling pathways, differentially combined defects which account for the expressed clinical manifestations.

15.
Front Pharmacol ; 12: 703965, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34557092

RESUMO

Xiaoyaosan (XYS), as a classic Chinese medicine compound, has been proven to have antidepressant effect in many studies, but its mechanism has not been clarified. In our previous studies, we found that chronic stress can induce depressive-like behavior and lead to emotion-related cingulate gyrus (Cg) dysfunction, as well as the decrease of neurotrophic factors and the increase of inflammatory-related proteins. Therefore, we speculated that XYS may play an antidepressant role by regulating the inflammation-related receptor of advanced glycation protein end product (RAGE) to affect the functional connectivity (FC) signal of the Cg and improve the depressive-like behavior. In order to verify this hypothesis, we analyzed the FC and RAGE expression in the Cg of depressive-like mice induced by chronic unpredictable mild stress (CUMS) and verified it with RAGE knockout mice. At the same time, we detected the effect of XYS on the depressive-like behavior, expression of RAGE, and the FC of the Cg of mice. The results showed that the FC of the Cg of depressive-like mice induced by CUMS was weakened, and the expression of RAGE was upregulated. The antidepressant effect of XYS is similar to that of fluoxetine hydrochloride, which can significantly reduce the depressive-like behavior of mice and inhibit the expression of the RAGE protein and mRNA in the Cg, and increase the FC of the Cg in mice. In conclusion, XYS may play an antidepressant role by downregulating the expression of RAGE in the Cg of depressive-like mice induced by CUMS, thereby affecting the functional signal and improving the depressive-like behavior.

16.
Talanta ; 235: 122774, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34517632

RESUMO

A method combining magnetic solid-phase extraction (MSPE) and ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed for the analysis of aristolochic acids I (AAI) in mouse serum and tissues. The magnetic covalent organic frameworks (MNP@COF)-based MSPE exhibited high adsorption capacity towards AAI (93.1 mg/g) in optimal conditions. After MSPE extraction, AAI was separated with C18 column using gradient elution and quantified (m/z 342.21 â†’ 298.13) by UHPLC-MS/MS with monitor reaction monitoring (MRM) mode. This MSPE-based UHPLC-MS/MS method was validated with respected to lower limit of quantification (LLOQ), linearity, recovery, precision and accuracy of intra- and inter-day, and matrix effect. Good calibration linearities at the range of 1-500 ng/L for AAI in biological matrices (serum, kidney, and liver) with high correlation coefficient (R2) > 0.9970, and high enrichment factors (mean values from 1038 to 1045) were obtained. This method was highly sensitive to determine AAI with LLOQ within the range of 4.62-5.24 ng/L in extracted serum, kidney, and liver samples. Recoveries at 5, 50, 100 and 300 ng/L in biological samples ranged from 93.2 to 104.0%, and intra- and inter day accuracy and precision (defined as bias and coefficient of variation, respectively) were below ± 15%. The method was successfully applied in the analysis of biological samples collected from mice exposed with AAI with concentrations range of 0.007-0.041 µg/L for consecutive four days. The established method might be applied for the investigation of risk assessment and toxicity induced by long-time use of AAI-containing herbs or dietary supplements.


Assuntos
Ácidos Aristolóquicos , Espectrometria de Massas em Tandem , Animais , Cromatografia Líquida de Alta Pressão , Fenômenos Magnéticos , Camundongos , Extração em Fase Sólida
17.
Nanomedicine (Lond) ; 16(26): 2389-2406, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34530631

RESUMO

Cancer is a malignant disease that seriously affects human health and life. Early diagnosis and timely treatment can significantly improve the survival rate of cancer patients. Surface-enhanced Raman scattering (SERS) is an optical technology that can detect and image samples at the single-molecule level. It has the advantages of rapidity, high specificity, high sensitivity and no damage to the sample. The performance of SERS is highly dependent on the properties, size and morphology of the SERS substrate. Preparation of SERS substrates with good reproducibility and chemical stability is a key factor in realizing the wide application of SERS technology in cancer diagnosis. In this review we provide a detailed presentation of the latest research on SERS in cancer diagnosis and the detection of cancer biomarkers, mainly focusing on nanotechnological approaches in cancer diagnosis by using SERS. We also consider the future development of nanostructure-based SERS in cancer diagnosis.


Assuntos
Nanoestruturas , Neoplasias , Humanos , Nanotecnologia , Neoplasias/diagnóstico por imagem , Reprodutibilidade dos Testes , Análise Espectral Raman
18.
Materials (Basel) ; 14(17)2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34501146

RESUMO

TiAl-based intermetallic alloys are prepared with Cu concentrations of 3-5 at.% (atomic ratio). The mechanical properties and microstructural characteristics of the alloys are investigated under static and dynamic loading conditions using a material testing system (MTS) and split-Hopkinson Pressure Bar (SHPB), respectively. The electrochemical properties of the various alloys are then tested in Ringer's solution. It is shown that the level of Cu addition significantly affects both the flow stress and the ductility of the samples. For Cu contents of 3 and 4 at.%, respectively, the flow stress and strain rate sensitivity increase at higher strain rates. Furthermore, for a constant strain rate, a Cu content of 4 at.% leads to an increased fracture strain. However, for the sample with the highest Cu addition of 5 at.%, the flow stress and fracture strain both decrease. The X-ray diffraction (XRD) patterns and optical microscopy (OM) images reveal that the lower ductility is due to the formation of a greater quantity of γ phase in the binary TiAl alloy system. Among all the specimens, that with a Cu addition of 4 at.% has the best anti-corrosion performance. Overall, the results indicate that the favourable properties of the TiAlCu4 sample stem mainly from the low γ phase content of the microstructure and the high α2 phase content.

19.
Sci Total Environ ; 801: 149803, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34467920

RESUMO

Acute cadmium toxicity induces multi-system organ failure. Mass spectrometry (MS)-based omics analyses and atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-MALDI MSI) are powerful tools for characterizing the biomarkers. Many studies on cadmium toxicity by metabolomics have been investigated, whereas the applications of lipidomics and MSI studies are still inadequate. In this study, the systematic metabolomics study on female ICR mice tissues including liver, kidney, heart, stomach, brain as well as spleen under cadmium exposure was firstly conducted and lipidomic characterizations on female ICR mice liver, kidney and heart were further constructed step by step. To deeply understand its toxicological mechanisms, several representative lipids on the mouse liver were visualized by AP-MALDI MSI. The results demonstrated that exposure to cadmium caused significant metabolic alterations in the liver, kidney and heart among all the tissues. Additionally, the toxicological mechanisms of cadmium in the mouse models are closely associated with the inflammation response, energy expenditure, oxidative stress, DNA and mitochondria damage, and lipid homeostasis. These insights could enhance knowledge in acute cadmium toxicity of public health and guide risk assessment in the future.


Assuntos
Cádmio , Lipidômica , Animais , Pressão Atmosférica , Cádmio/toxicidade , Feminino , Camundongos , Camundongos Endogâmicos ICR , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
20.
Sci Total Environ ; 798: 149379, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34375234

RESUMO

Cadmium (Cd), a toxic heavy mental, has been reported to be correlated with increased incidences of multiple diseases. Only a few studies have paid attention to screen the urine metabolites related to long-term environmental Cd exposure in humans. Research on the Cd exposure-related serum metabolic alternations and biological mechanisms linking Cd exposure to adverse health risks in humans is scanty. In this study, we investigated the serum Cd exposure-related metabolic alternations in a cohort of 101 non-smoking females (two polluted groups and one control group) and 18 Cd exposure-related metabolites were identified. A total of 16 clinical indicators of renal and hepatic functions and bone health were measured. Five health effect biomarkers including serum creatinine, alkaline phosphatase, total bilirubin, direct bilirubin and albumin to globulin ratio that are related to impaired renal and hepatic functions showed significant differences among the three groups and had close correlations with Cd levels. We identified intermediate metabolites that were associated with both Cd exposure and health effect biomarkers using a "meet-in-the-middle" approach. Fourteen Cd exposure-related metabolites in the metabolism of glycerophospholipids, sphingolipids, arachidic acid, linoleic acid and amino acids, were identified to be the intermediates of Cd exposure and the health effect biomarkers. Our findings provided evidence for the linkage of long-term environmental Cd exposure and the renal and hepatic insufficiency.


Assuntos
Cádmio , Hepatopatias , Biomarcadores , Cádmio/toxicidade , China , Exposição Ambiental , Feminino , Humanos , Rim
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