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1.
Medicine (Baltimore) ; 99(12): e19496, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32195949

RESUMO

BACKGROUND: Dysmenorrhea seriously affects the ability of women to perform normal social activities and decreases their quality of life. Primary dysmenorrhea can be effectively treated with acupuncture. Based on the wrist-ankle acupuncture (WAA) theory, we designed a portable WAA point compression treatment strap that treats diseases by automatically applying pressure to acupuncture points. The proposed study aims to evaluate the immediate analgesic effect of the acupressure wrist-ankle strap in patients with primary dysmenorrhea. METHODS: The study will be a randomized controlled trial conducted from May 1, 2019 to May 30, 2020 that includes 78 students from Shanghai University of Traditional Chinese Medicine who have primary dysmenorrhea and meet the eligibility criteria. Participants will be randomly divided into 2 groups in a 1:1 allocation ratio. The intervention group will use the acupressure wrist-ankle strap equipped with tip compression component parts on the internal side; the control group will use the nonacupressure wrist-ankle strap with the tip compression parts removed. All participants will be treated for 30 minutes on the 1st day of menstruation. The primary outcome is the pain intensity score measured by the visual analog scale. The secondary outcomes are the onset time of analgesia, the pain threshold at Yinlingquan (SP 9), skin temperature at Guanyuan (CV 4), and expectations and satisfaction of patients as investigated via the expectation and treatment credibility scale. DISCUSSION: This trial will be the 1st study to evaluate the analgesic effect of the acupressure wrist-ankle strap in patients with primary dysmenorrhea. The quality of this study is ensured by the randomization, nonacupressure control, and blinded design. The results may provide evidence for a potential alternative treatment for primary dysmenorrhea and evidence-based proof of the analgesic effect of WAA.


Assuntos
Acupressão/efeitos adversos , Pontos de Acupuntura , Terapia por Acupuntura/métodos , Dismenorreia/terapia , Analgesia por Acupuntura/instrumentação , Analgesia por Acupuntura/estatística & dados numéricos , Adolescente , Adulto , Tornozelo , China/epidemiologia , Dismenorreia/epidemiologia , Dismenorreia/psicologia , Feminino , Humanos , Limiar da Dor , Satisfação do Paciente , Qualidade de Vida , Escala Visual Analógica , Punho , Adulto Jovem
2.
World J Gastroenterol ; 26(8): 789-803, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32148377

RESUMO

BACKGROUND: Despite significant advances in multimodality treatments, hepatocellular carcinoma (HCC) remains one of the most common malignant tumors. Identification of novel prognostic biomarkers and molecular targets is urgently needed. AIM: To identify potential key genes associated with tumor microenvironments and the prognosis of HCC. METHODS: The infiltration levels of immune cells and stromal cells were calculated and quantified based on the ESTIMATE algorithm. Differentially expressed genes (DEGs) between high and low groups according to immune or stromal scores were screened using the gene expression profile of HCC patients in The Cancer Genome Atlas and were further linked to the prognosis of HCC. These genes were validated in four independent HCC cohorts. Survival-related key genes were identified by a LASSO Cox regression model. RESULTS: HCC patients with a high immune/stromal score had better survival benefits than patients with a low score. A total of 899 DEGs were identified and found to be involved in immune responses and extracellular matrices, 147 of which were associated with overall survival. Subsequently, 52 of 147 survival-related DEGs were validated in additional cohorts. Finally, ten key genes (STSL2, TMC5, DOK5, RASGRP2, NLRC3, KLRB1, CD5L, CFHR3, ADH1C, and UGT2B15) were selected and used to construct a prognostic gene signature, which presented a good performance in predicting overall survival. CONCLUSION: This study extracted a list of genes associated with tumor microenvironments and the prognosis of HCC, thereby providing several valuable directions for the prognostic prediction and molecular targeted therapy of HCC in the future.

3.
Transplant Proc ; 52(1): 345-352, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31918969

RESUMO

BACKGROUND AND OBJECTIVE: The aim of this study was to investigate the effects of granulocyte colony-stimulating factor (G-CSF) on the proliferation and apoptosis of bone marrow (BM) B cells from healthy donors and its mechanism. MATERIALS AND METHODS: The proliferation ability and apoptosis of BM cells from healthy donors before and after in vivo G-CSF application were determined by multiparameter flow cytometry. The gene expression of B cells was detected by RNA-Seq. In vitro experiments were performed to investigate the effects of G-CSF on the proliferation and apoptosis of BM B cells through which gene. RESULTS: Treating healthy donors with G-CSF significantly decreased proliferation and increased apoptosis of BM B cells. The proliferation of CD19+CD27- B cell subgroup and CD19+CD24hiCD38hi B cell subset were also decreased. G-CSF also significantly altered proapoptotic genes, cell cycle arrest genes, and DNA replication and cell cycle genes, especially significantly increased SOCS1 expression of BM B cells. In vitro experiments showed that SOCS1 overexpression did not affect B cell proliferation ability and apoptosis. CONCLUSIONS: Our results suggest that extensive effects of G-CSF on BM B cells, such as inhibiting proliferation, inducing apoptosis, and altering a series of gene expression.

4.
J Hazard Mater ; 381: 120961, 2020 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-31412305

RESUMO

We design a novel cationic metal-organic framework hybrid ultrafiltration polyvinylidene fluoride membrane (PVA/Cu-iMOFs/PVDF-0.05) and report its unique capture of aqueous perchlorate (ClO4-) at ppm-level. This membrane outperformed traditional adsorption materials and exhibited a specific affinity toward ClO4- in the presence of various competing anions at greater levels (up to a concentration ratio of 20). In the batch experiment, the ClO4- removal ratio reached 99.6% over a wide pH range (3˜10). Membrane filtration by using a 12.56 cm2 PVA/Cu-iMOFs/PVDF-0.05 membrane could effectively treat 4.71 L of ClO4--contaminated water before breakthrough occurred, while maintaining a satisfactory permeability (˜627.32 L/(m2 h bar)) and antifouling property. The exhausted membrane could easily be regenerated in aminoethanesulfonic acid solution for repeated use with a negligible decrease in capacity. Moreover, the membrane showed excellent long-term stability in a cross-flow filtration process due to the amido bond between the Cu-iMOFs and membrane surface as well as the "protection" of polyvinyl alcohol. Selective and reversible ion-exchange between the sulfonic acid (R-SO3) ligands of Cu-iMOFs and tetrahedral oxo-anionic species was verified to be the pathway for ClO4- trapping. Thus, other problematic elements that also occur in tetrahedral form in water can be removed by this method.

5.
J Hematol Oncol ; 12(1): 31, 2019 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-30885244

RESUMO

INTRODUCTION: Myeloid-derived suppressor cells (MDSCs) are proposed to control graft-versus-host disease (GVHD) in allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, the definition of human MDSCs has not yet reached consensus, and the mechanism of MDSCs to control GVHD remains unclear. METHODS: Immature myeloid cells (HLA-DR-/lowCD33+CD16-) were tested before and after granulocyte colony-stimulating factor (G-CSF) administration in healthy donor and isolated for suppression assays and co-culture with T cells in vitro. Isolated cells were infused in humanized mice for a xenogeneic model of acute GVHD. One hundred allo-HSCT recipients were enrolled prospectively to assess the role of HLA-DR-/lowCD33+CD16- cells in grafts on the occurrence of acute GVHD. RESULTS: In the present study, G-CSF mobilized HLA-DR-/lowCD33+CD16- cells with immunosuppressive properties in donor peripheral blood. These cells contained more interleukin-10+ and transforming growth factor-beta (TGF-ß)+ cells after G-CSF administration and inhibited the proliferation of autologous donor T cells in a TGF-ß-dependent manner. Meanwhile, these immature myeloid cells promoted regulatory T cell expansion and induced Th2 differentiation. Importantly, these cells prevented acute GVHD in a humanized mouse model. Moreover, clinical cohort results showed that the number of HLA-DR-/lowCD33+CD16- cells in the donor graft was the only independent risk factor inversely correlated with the incidence of grade II-IV acute GVHD in the recipients (HR 0.388, 95% CI 0.158-0.954, p = 0.039). CONCLUSION: HLA-DR-/lowCD33+CD16- cells represent functional MDSCs that may control acute GVHD in allo-HSCT.

6.
Bone Marrow Transplant ; 54(8): 1287-1294, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30655602

RESUMO

Donor-specific anti-human leukocyte antigen (HLA) antibodies (DSAs) were associated with graft failure (GF) following haploidentical stem cell transplantation (Haplo-HSCT). The prevalence and risk factors of DSAs in pediatric candidates remain to be determined. In a prospective trial (ChiCTR-OPC-15006672), 486 children with hematological diseases were enrolled to screen for the presence of anti-HLA class I and II antibodies of immunoglobulin G type. Fifty two patients (10.7%) demonstrated positive panel-reactive antibody (PRA) for class I; 24 (4.9%), for class II; and 13 (2.7%), for both. Multivariate analysis showed diagnosis was the independent risk factor for antibodies, as acute lymphoblastic leukemia (ALL) patients (HR0.141, 95% CI: 0.037-0.538, p = 0.004) had a lower incidence of class II PRAs and DSAs against HLA-B, DQ, and DR, whereas myelodysplastic syndrome (MDS) patients had a higher incidence of PRAs for both class I and class II (HR4.790, 95% CI: 1.010-22.716, p = 0.049), and DSAs against HLA-A, B, C, DP, and DQ. Older age (>12 vs. ≤12) was associated with DSAs against HLA-DP (HR0.194, 95% CI: 0.041-0.918, p = 0.039). Our findings provided novel evidence for prevalence and risk factors for PRAs and DSAs in pediatric candidates receiving haplo-HSCT, possibly benefiting anti-HLA antibody monitoring and donor selection.

7.
BMC Nephrol ; 19(1): 245, 2018 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-30241513

RESUMO

BACKGROUND: We herein report a 3-year-old boy presented with chronic kidney disease (CKD) due to PAX2 missense mutation (C to G transversion at position 418 in exon 4). CASE PRESENTATION: He attended our clinic with a 3-month history of foamy urine. Upon examination, he had reduced estimated glomerular filtration rate (GFR) and renal atrophy. Genetic investigations revealed that he has inherited a mutated PAX2 gene from his father, who had renal failure at the age of 20. We searched the literature and confirmed that this mutation site has not been reported by any other group before. CONCLUSIONS: Although renal coloboma syndrome (RCS) with simultaneous kidney and eye involvement is the most common phenotype of PAX2 mutations, current literature supports that such mutations may have profuse clinical manifestations and renal hypoplasia is one distinct entity in the spectrum.


Assuntos
Heterozigoto , Mutação de Sentido Incorreto , Fator de Transcrição PAX2/genética , Insuficiência Renal Crônica/genética , Pré-Escolar , Taxa de Filtração Glomerular , Humanos , Masculino , Insuficiência Renal Crônica/diagnóstico
8.
Chemosphere ; 211: 139-148, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30071425

RESUMO

Chemical cleaning is an important approach for alleviating severe fouling in membrane separation processes. In this study, lysozyme (LYS) was exposed to sodium hypochlorite (NaClO) with varied concentrations (0-2000 ppm) to understand the changes in the physicochemical properties and functional groups as well as the variations in membrane permeabilities. The results showed that membrane filterability exhibited an obvious 'U-shaped' trend, and the valley existed when the ratio of Cl/C (the ratio of NaClO and TOC concentrations in feed water) is among 1.35-3.09. Upon exposure to low dose NaClO, three-dimensional fluorescence excitation-emission matrix (3D-EEM) spectra showed that tryptophan protein substances were transformed to more hydrophobic humic-like substances. Fourier transform infrared (FTIR) analysis further confirmed that exposure to low dose NaClO promoted the breakage of aromatic substituents, leading to the formation of hydrophobic condensed aromatic substances. On the contrary, at high NaClO loads, protein structures were destroyed completely and almost no obvious fluorescent intensities could be detected, which promoted the recovery of membrane filterabilities. Notably, the chemical cleaning mechanisms of fouled membranes with NaClO were understood in depth in this study. These results provide new information about the oxidation products of LYS and the cleaning efficiency upon exposure to NaClO.


Assuntos
Permeabilidade , Hipoclorito de Sódio/química , Purificação da Água/métodos , Interações Hidrofóbicas e Hidrofílicas
9.
Water Res ; 143: 87-98, 2018 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-29940365

RESUMO

Enhanced removal of trace toxic metals (ppm level) from high-salinity wastewater is crucial to ensure water safety but still a challenging task. In this study, we fabricated a new hybrid ultrafiltration membrane (PAA/ZIF-8/PVDF) by immobilizing zeolitic imidazolate framework-8 (ZIF-8) particles onto the surface of trimesoyl chloride (TMC)-modified polyvinylidene fluoride (PVDF) membrane under protection of polyacrylic acid (PAA) layer. The resultant PAA/ZIF-8/PVDF membrane exhibited relatively high water flux of 460 L·m-2 h-1 and outstanding nickel ion (Ni(II)) capacity (219.09 mg/g) from a synthetic high-salinity ([Na+] = 15000 mg/L) wastewater. X-ray photoelectron spectroscopic studies revealed that preferable Ni(II) uptake was mainly attributed to the specific interaction between Ni(II) and hydroxyl groups on ZIF-8 frameworks and carboxyl groups in PAA layer as well. Compared to PAA, ZIF-8 could selectively bind Ni(II) with negligible effect exerted by concentrated sodium ion. The filtration study showed that the 12.56-cm2 membrane could effectively treat 5.76 L high-salinity wastewater ([Ni(II)0 = 2 mg/L, [Na+]0 = 15000 mg/L) to conspicuously reduce Ni(II) below the maximum contaminant level of China, 0.1 mg/L. Moreover, the hybrid membrane could be regenerated by HCl-NaCl solution (pH = 5.5) for repeated use under direct current electric field. Generally speaking, the newly developed ZIF-8 hybrid ultrafiltration membrane showed a very promising potential in enhanced removal of toxic metals from high-salinity wastewater treatment.


Assuntos
Membranas Artificiais , Níquel/isolamento & purificação , Ultrafiltração/instrumentação , Águas Residuárias/química , Poluentes Químicos da Água/isolamento & purificação , Resinas Acrílicas/química , Adsorção , Concentração de Íons de Hidrogênio , Imidazóis/química , Estruturas Metalorgânicas/química , Níquel/química , Espectroscopia Fotoeletrônica , Polivinil/química , Salinidade , Espectroscopia de Infravermelho com Transformada de Fourier , Ultrafiltração/métodos , Poluentes Químicos da Água/química , Difração de Raios X , Zeolitas/química
10.
Hum Immunol ; 79(9): 672-677, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29890181

RESUMO

We investigated the prevalence of and risk factors for antibodies to HLA in 1663 haploidentical transplant candidates. Among these cases, 349 (21.0%) showed positive panel-reactive antibody (PRA) either for class I or class II HLA. Multivariate analysis showed the following: i) risk factors associated with the prevalence of PRA either for class I or class II HLA were female gender (P = 0.018), prior transfusions (P < 0.001) or pregnancy (P < 0.001), and cases with MDS (P = 0.018); compared to other patients, subjects with ALL had a lower prevalence of class I antibodies (P = 0.017); and ii) risk factors associated with the prevalence of PRA both for class I and class II HLA were female gender (P = 0.014), prior transfusions (P = 0.003), previous pregnancy (P < 0.001), and diagnosis with MDS (P = 0.035). The percentages of antibodies against different antigens coded by the different HLA loci, including HLA-A, -B, -C, -DP, -DQ, and -DR, among all cases were 15.6%, 17.3%, 10.5%, 5.6%, 8.5%, and 9.7%, respectively. Risk factors associated with specific antibodies against HLA-A, -B, -C, -DP, -DQ, and -DR were female gender, prior transfusion, previous pregnancy, and underlying disease. Our findings suggest that gender, prior pregnancy, transfusion and underlying diseases are risk factors for HLA sensitization, which could guide HLA antibody monitor and donor selection.


Assuntos
Antígenos HLA/genética , Isoanticorpos/biossíntese , Transplante de Células-Tronco , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China/epidemiologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Transplantados , Transplante Haploidêntico , Listas de Espera , Adulto Jovem
11.
Biochem Genet ; 56(3): 235-254, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29350309

RESUMO

Ottelia acuminata is an edible aquatic plant species that is endemic to southwestern China. This plant has experienced habitat degradation resulting from environmental change and extensive human disturbance. Determining the genetic variation and genetic structure of O. acuminata populations could help develop strategies to collect, evaluate, utilize and conserve the species. To this end, we genotyped 183 individuals sampled throughout the species distribution using twelve novel nuclear microsatellite loci (nSSRs). Eight of these nSSRs exhibited low average levels of genetic diversity (HE = 0.351, Ho = 0.376) and showed evidence of significant inbreeding across several populations. A high degree of genetic differentiation was identified among populations (FST = 0.457), probably resulting from limited pollen and seed-mediated gene flow. Only 17.8% of variation existed between O. acuminata var. acuminata and other O. acuminata varieties. Bayesian analysis and a UPGMA dendrogram based on Nei's genetic distance also revealed notably low genetic differentiation among the varieties. This low genetic differentiation is possibly attributed to shared ancestral polymorphisms since their divergence. Additional taxonomic and phylogenetic studies with additional molecular markers are needed to determine the population genetic relationship between O. acuminata varieties. Conservation of this species depends on in situ and ex situ actions, such as controlling habitat water pollution and overexploitation and creating a germplasm bank based on the population genetic differences. To the best of our knowledge, this study represents the first attempt to understand the population genetics of O. acuminata in China using novel nSSR markers developed from transcriptome sequencing and could contribute to the conservation management of this economic plant.


Assuntos
Fluxo Gênico , Hydrocharitaceae/genética , Repetições de Microssatélites , Filogenia , Polimorfismo Genético , Sementes/genética , China , Genética Populacional
12.
Minim Invasive Ther Allied Technol ; 27(3): 164-170, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28697642

RESUMO

BACKGROUND: Laparoscopic distal gastrectomy (LDG) for gastric cancer has gradually gained popularity. However, laparoscopic total gastrectomy (LTG) has been reported rarely when compared with LDG. This study was designed to evaluate the surgical outcomes as well as the morbidity and mortality of LTG compared with LDG to confirm the feasibility and safety of LTG. MATERIAL AND METHODS: We reviewed the data of patients at our institution undergoing LTG (n = 448) or LDG (n = 956) for gastric cancer between January 2008 and July 2016. Then the clinical characteristics and perioperative clinical outcomes of the two groups were compared. RESULTS: Except for tumor size and stage, there were no statistically significant differences in the clinicopathological parameters between the groups. LTG was associated with significantly longer operation time, late time to postoperative diet, and longer hospital stay compared with the LDG group. Overall complications developed in 60 patients (13.4%) and surgical complications in 48 patients (10.7%) after LTG. Postoperative complications were less frequent in the LDG group than in the LTG group (8.4% versus 13.4%, p < .01), and fewer surgical complications were observed with LDG than with LTG (7.5% versus 10.7%, p = .05). CONCLUSIONS: The results of LTG were favorable even though are not inferior to those of LDG. LTG for gastric cancer is technically feasible and safe. However, because of the limits of this study, other high-quality studies are needed for further evaluation.


Assuntos
Adenocarcinoma/cirurgia , Gastrectomia/métodos , Neoplasias Gástricas/cirurgia , Idoso , Estudos de Viabilidade , Feminino , Gastrectomia/estatística & dados numéricos , Humanos , Laparoscopia/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
13.
Medicine (Baltimore) ; 96(38): e8061, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28930841

RESUMO

Although surgical outcomes of totally laparoscopic total gastrectomy (TLTG) have been reported from several centers, the effectiveness of this technique has not been conclusively established. The aim of this study was to investigate the feasibility, safety, and efficacy of TLTG for gastric cancer.A prospectively collected and retrospectively analyzed data were used by comparing the short-term surgical outcomes of 124 patients who underwent TLTG with those of 124 patients who underwent open total gastrectomy (OTG) between March 2007 and March 2016.The 2 groups were well matched with respect to age, sex, body mass index, ASA score, and tumor stage. There was no significant difference with regard to the operation time but TLTG showed significantly less intraoperative blood loss (115.5 ±â€Š70.2 vs 210.5 ±â€Š146.7 mL, P < .01). Total numbers of retrieved lymph nodes were similar in the 2 groups. Postoperatively, no significant differences were found for morbidity or mortality. The time to first flatus, initiate oral intake, and postoperative hospital stay were significantly shorter in the TLTG group than in the OTG group (3.2 ±â€Š1.0 vs 4.1 ±â€Š1.2 days; 4.4 ±â€Š1.2 vs 5.6 ±â€Š2.0 days; and 8.9 ±â€Š3.1 vs 11.3 ±â€Š4.5 days, respectively; P < .01).TLTG for gastric cancer is technically safe, feasible, and minimally invasive compared with OTG. A prospective randomized trial is needed to establish the value of TLTG.


Assuntos
Gastrectomia/métodos , Laparoscopia/métodos , Neoplasias Gástricas/cirurgia , Feminino , Gastrectomia/efeitos adversos , Humanos , Laparoscopia/efeitos adversos , Tempo de Internação , Excisão de Linfonodo , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Complicações Pós-Operatórias , Estudos Retrospectivos , Neoplasias Gástricas/patologia
14.
BMC Gastroenterol ; 17(1): 78, 2017 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-28629379

RESUMO

BACKGROUND: Obesity is a growing epidemic around the world, and obese patients are generally regarded as high risk for surgery compared with normal weight patients. The purpose of this study was to evaluate the influence of obesity on the surgical outcomes of laparoscopic gastrectomy (LG) for gastric cancer. METHODS: We reviewed data for all patients undergoing LG for gastric cancer at our institute between October 2004 and December 2016. Patients were divided into non-obese and obese groups and the perioperative outcomes were compared. Furthermore, a subgroup analysis was conducted to evaluate which of the two commonly used methods of LG, laparoscopic-assisted gastrectomy (LAG) and totally laparoscopic gastrectomy (TLG), is more suitable for obese patients. RESULTS: A total of 1691 patients, 1255 non-obese and 436 obese or overweight patients, underwent LG during the study period. The mean operation time was significantly longer in the obese group than in the non-obese group (209.9 ± 29.7 vs. 227.2 ± 25.7 min, P < 0.01), and intraoperative blood loss was significantly lower in the non-obese group (113.4 ± 34.1 vs. 136.9 ± 36.7 ml, P < 0.01). Time to first flatus, time to oral intake, and postoperative hospital stay were significantly shorter in the non-obese group than in the obese group (3.3 ± 0.8 vs. 3.6 ± 0.9 days; 4.3 ± 1.0 vs. 4.6 ± 1.0 days; and 9.0 ± 2.2 vs. 9.6 ± 2.2 days, respectively; P < 0.01). 119 (9.5%) of the non-obese patients had postoperative complications as compared to 44 (10.1%) of the obese patients (P = 0.71). In the subgroup analysis of all patients, TLG showed improved results for early surgical outcomes compared to LAG, mainly due to its advantages in obese patients. CONCLUSIONS: Obesity is associated with long operation time, increased blood loss, and slow recovery after laparoscopic gastric resection but does not affect intraoperative security or effectiveness. TLG may have less negative results in obese patients than LAG due to a variety of reasons. Our analysis shows that TLG is more advantageous, with regard to early surgical outcomes, for obese patients.


Assuntos
Gastrectomia/métodos , Laparoscopia/métodos , Obesidade/cirurgia , Neoplasias Gástricas/cirurgia , Idoso , Perda Sanguínea Cirúrgica/estatística & dados numéricos , Bases de Dados Factuais , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Obesidade/complicações , Duração da Cirurgia , Período Pós-Operatório , Estudos Prospectivos , Estudos Retrospectivos , Neoplasias Gástricas/complicações , Resultado do Tratamento
15.
BMC Surg ; 17(1): 33, 2017 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-28376760

RESUMO

BACKGROUND: Laparoscopic resections for submucosal tumors (SMTs) of the stomach have been developed rapidly over the past decade. Several types of laparoscopic methods for gastric SMTs have been created. We assessed the short-term outcomes of two commonly used types of laparoscopic local resection (LLR) for gastric SMTs and reported our findings. METHODS: We retrospectively analyzed the clinicopathological results of 266 patients with gastric SMTs whom underwent LLR between January 2006 and September 2016. 228 of these underwent laparoscopic exogastric wedge resection (LEWR), the remaining 38 patients with the tumors near the esophagogastric junction (EGJ) or antrum underwent laparoscopic transgastric resection (LTR). RESULTS: All the patients underwent laparoscopic resections successfully. The mean operation times of LEWR and LTR were 90.2 ± 37.2 min and 101.7 ± 38.5 min respectively. The postoperative length of hospital stays for LEWR and LTR were 5.1 ± 2.1 days and 5.3 ± 1.7 days respectively. There was a low complication rate (4.4%) and zero mortality in our series. CONCLUSION: ELWR is technically feasible therapy of gastric SMTs. LTR is secure and effective for gastric intraluminal SMTs located near the EGJ or antrum.


Assuntos
Gastrectomia/métodos , Laparoscopia/métodos , Neoplasias Gástricas/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Junção Esofagogástrica/patologia , Feminino , Mucosa Gástrica/patologia , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
16.
J Med Food ; 19(8): 798-804, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27533651

RESUMO

The ancient Chinese wild rice (Zizania latifolia (Griseb) Turcz) (CWR) has valuable biological and medicinal functions. To assess the advantages lost in modern cultivated rice after domestication, we compared the composition of bioactive compounds and the results of proteomic analysis with those of Indica rice (N22). We used routine methods to determine the protein, total dietary fiber, amino acid, mineral substance, plant secondary metabolites, and amino acid composition of CWR and N22. The protein and mineral contents of CWR were two times that of N22, and the levels of calcium, potassium, magnesium, chromium, iron, and zinc were significantly higher than those of N22 (P < .05). There was ∼7.6 times more dietary fiber in CWR than in N22, but fewer carbohydrates (P < .05). Anthocyanins and chlorophyll were detected in CWR, but were absent from N22. Compared with N22, CWR had 53, 19, and 5.4 times higher (P < .05) levels of saponins, flavonoids, and plant sterols, respectively. The amino acid score of CWR was 66.6, which was significantly higher than N22. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that the main seed proteins of CWR were glutelins, including both acid and alkaline subunits, which were approximately twice those of N22. To investigate the differences in protein profiles between CWR and N22, we conducted two-dimensional electrophoresis (2-DE) analysis of the total proteins in the seeds of the two rice species. 2-DE gels revealed 19 differentially expressed proteins. Information obtained from peptide mass fingerprinting indicates that glutelin precursor caffeoyl coenzyme A (CoA) O-methyltransferase and putative bithoraxoid-like protein can provide good gene sources for improving rice quality.


Assuntos
Proteínas na Dieta/análise , Domesticação , Grão Comestível/química , Valor Nutritivo , Oryza/química , Proteínas de Plantas/análise , Sementes/química , Antocianinas/análise , China , Clorofila/análise , Glutens/análise , Poaceae , Proteômica
17.
Minerva Med ; 107(5): 322-7, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27285120

RESUMO

INTRODUCTION: The aim of this study was to determine the role of Prognostic Nutritional Index in predicting gastric cancer. EVIDENCE ACQUISITION: Studies were searched in PubMed, EMBASE and ISI Web of Science. The hazard ratios and 95% confidence interval for survival estimate were extracted from the articles and the pooled HR was computed. EVIDENCE SYNTHESIS: Nine studies with 3203 participants met the inclusion criteria. Low PNI was associated with poor overall survival (pooled HR 1.92, 95% CI: 1.68-2.19). CONCLUSIONS: Prognostic Nutritional Index was an effective indicator for the prognosis of gastric cancer.


Assuntos
Avaliação Nutricional , Neoplasias Gástricas/mortalidade , Humanos , Prognóstico , Taxa de Sobrevida
18.
Huan Jing Ke Xue ; 34(10): 3862-71, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24364304

RESUMO

A field experiment was carried out in Zhushanhu in September, 2011. On the basis of mass balance, nutrients flow in and out of Zhushanhu and their Digestion-absorption law was illustrated through water quantity-water quality observation of bay heart, bay mouth and rivers around Zhushanhu, which provides basic data for the further research on the self-purification capacity of Lake Taihu. The EcoTaihu model was adopted to simulate the nutrients flow and their self-purification capacity of Lake Taihu. The simulated annual self-purification capacity of total nitrogen and total phosphorus of Zhushanhu was 1 911 t and 116 t, respectively, whereas the observed annual self-purification capacity of total nitrogen and total phosphorus of Zhushanhu was 1 979 t and 119 t, respectively. The model was validated by the observation data. The simulated result showed that the self-purification capacity of total nitrogen of Lake Taihu in year 2006, 2008 and 2010 was 4. 00 x 10(4) t, 4. 27 x 10(4) t and 4. 11 x 10(4) t, respectively, whereas the self-purification capacity of total phosphorus of Lake Taihu in year 2006, 2008 and 2010 was 1.56 x 10(3) t, 1.80 x 10(3) t and 1.71 x 10(3) t, respectively.


Assuntos
Monitoramento Ambiental , Lagos/química , Modelos Teóricos , Nitrogênio/química , Fósforo/química , China , Qualidade da Água
19.
Int J Ophthalmol ; 6(6): 805-10, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24392329

RESUMO

AIM: To examine the association between dietary macronutrient intake and the risk of age-related cataract (ARC) in middle-aged and elderly men. METHODS: A hospital-based case-control study was conducted from December 2009 to November 2011. Cases (n=360) were patients with cataract aged 45-85 years old, and controls (n=360) were patients who had been admitted to the same hospital for diseases not related with cataract. All subjects were interviewed using a structured interviewer-administrated questionnaire that included information on socio-demographic characteristics, lifestyle habits and detailed medical history, simultaneously, the dietary intakes of nutrients were collected via a valid semi-quantitative food frequency questionnaire (FFQ). The odds ratios (OR) and corresponding 95% confidence intervals (CI) of three types of ARC were estimated using multiple logistic regression models. RESULTS: After adjusting for multiple potential confounders, total dietary intake of carbohydrate was positively associated with cortical cataract, compared to controls in the lowest quartile, and the OR for cases in the highest quartile of intake was 2.471 (95%CI: 1.348-6.043, P=0.027). Higher dietary intakes of protein were protective for posterior subcapsular cataract (PSC) (OR=0.528, 95%CI: 0.148-0.869, P=0.023). Dietary fat intake was not associated with any type of cataract, however, participants in the highest quartile of polyunsaturated fatty acids intake had 2.7 times the risk of nuclear cataract as did those in the lowest quartile (OR=2.742, 95%CI: 1.790-4.200, P=0.033). CONCLUSION: A high intake of carbohydrate and polyunsaturated fatty acid may increase the odds of cortical and nuclear cataract, respectively, whereas high intake of protein, especially animal protein, may protect against PSC cataract. It is possible that dietary changes of target population may reduce the risk of ARC.

20.
Zhongguo Dang Dai Er Ke Za Zhi ; 14(12): 893-7, 2012 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-23234772

RESUMO

OBJECTIVE: To investigate the effect of pulmonary surfactant (PS) on the Th1/Th2 balance and serum levels of interleukin-4 (IL-4), interferon-γ (IFN-γ) and IgE in neonates with respiratory distress syndrome (RDS). METHODS: A total of 58 neonates with RDS were divided into control (n=20) and PS treatment groups (n=38). The control group underwent mechanical ventilation and other conventional treatment, while the PS treatment group received with bovine PS treatment within 1 hour of being admitted to the hospital together with mechanical ventilation and other conventional treatment. Enzyme-linked immunosorbent assay was used to measure serum levels of IL-4, IFN-γ and IgE before treatment and 24, 48 and 72 hours after treatment. Simultaneously, arterial blood gas, respiratory system compliance, and other ventilator parameters were recorded. RESULTS: Compared with the control group, the PS treatment group showed significantly shorter duration of mechanical ventilation and oxygen exposure time (P<0.05), significantly better respiratory system compliance and significantly lower oxygenation index 24, 48 and 72 hours after treatment (P<0.05). At 48 and 72 hours after treatment, serum levels of IFN-γ were significantly lower in the PS treatment group than in the control group (120±46 ng/L vs 229±59 ng/L, P<0.05; 141±40 ng/L vs 282±44 ng/L, P<0.05), and serum levels of IL-4 were significantly higher in the PS treatment group than in the control group (263±48 pg/mL vs 152±45 pg/mL, P<0.05; 417±49 pg/mL vs 201±46 pg/mL, P<0.05). At 72 hours after treatment, serum level of IgE was significantly lower in the PS treatment group than in the control group (115±44 pg/mL vs 199±43 ng/mL; P<0.05). CONCLUSIONS: PS treatment can shorten the duration of mechanical ventilation and oxygen exposure time, regulate serum levels of IFN-γ, IL-4 and IgE, and influence Th1/Th2 balance in neonates with RDS, thus inhibiting lung inflammatory response and reducing lung injury.


Assuntos
Surfactantes Pulmonares/uso terapêutico , Síndrome do Desconforto Respiratório do Recém-Nascido/tratamento farmacológico , Células Th1/imunologia , Células Th2/imunologia , Contagem de Linfócito CD4 , Feminino , Humanos , Imunoglobulina E/sangue , Recém-Nascido , Interferon gama/sangue , Interleucina-4/sangue , Masculino , Surfactantes Pulmonares/farmacologia , Respiração Artificial , Síndrome do Desconforto Respiratório do Recém-Nascido/complicações , Síndrome do Desconforto Respiratório do Recém-Nascido/imunologia
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