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1.
Food Funct ; 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-32243488

RESUMO

Selenium (Se) as an essential micronutrient plays a crucial role in human health. Biogenic selenium nanoparticles (SeNPs) possess attractive biological properties, biocompatibility, stability and low-toxicity. This study was aimed to investigate the protective effect of biogenic SeNPs of size 50-80 nm synthesized by Lactobacillus casei ATCC 393 (L. casei ATCC 393) on diquat-induced intestinal barrier dysfunction in C57BL/6 mice and the intrinsic mechanisms. Our results showed that oral administration of SeNPs significantly inhibited the increase of the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), diamine oxidase (DAO) and d-lactic acid (d-LA) levels induced by diquat, and increased the total superoxide dismutase (T-SOD), thioredoxin reductase (TrxR) and glutathione peroxidase (GSH-Px) activities in serum and jejunum. Moreover, SeNPs increased the number of goblet cells, decreased the production of reactive oxygen species (ROS), maintained the mitochondrial functions, and improved the expression levels of occludin and claudin-1 in jejunum compared to the diquat-induced oxidative stress model group. In addition, SeNPs activated the nuclear factor (erythroid-derived-2)-like 2 (Nrf2), and improved the protein levels of heme oxygenase (HO)-1 and NADPH dehydrogenase (NQO)-1 compared to other treatment groups. These results suggested that biogenic SeNPs synthesized by L. casei ATCC 393 can protect the intestinal barrier function against oxidative damage via Nrf2-mediated signaling pathway.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32139295

RESUMO

BACKGROUND: Transarterial chemoembolization (TACE) and percutaneous microwave coagulation therapy (PMCT) are commonly used to treat intrahepatic recurrent liver cancers. However, there is no information regarding their effectiveness in patients with recurrent intrahepatic cholangiocarcinoma (ICC) after resection. METHODS: A total of 275 patients with localized recurrent ICC who received either TACE (n = 183) or PMCT (n = 92) were studied. A propensity score matching analysis was performed to compare prognostic impact of TACE and PMCT. Prognostic factors for TACE and PMCT were identified respectively. Predictive nomograms for each TACE and PMCT were developed using the Cox independent prognostic factors and were validated in independent patient groups by receiver operating characteristic curves and area under curve values. RESULTS: Both TACE and PMCT provided curativeness in partial patients (5-year overall survival: 21.4% and 6.1%, respectively), but TACE provided better survival benefit in both overall patients (hazard ratio [HR] = 0.71; 95% confidence interval [CI]: 0.50-0.97; P = 0.034) and propensity score matching analysis (HR = 0.69; 95% CI: 0.47-0.98; P = 0.041). Independent prognostic factors for TACE were tumor size >5 cm, poor differentiation, and major resection, whereas poor differentiation, hepatitis B virus infection, cholelithiasis, and lymph node metastasis were identified for PMCT. Both predictive nomograms for TACE and PMCT were validated to be effective with area under curve values of 0.77 and 0.70, respectively. CONCLUSIONS: TACE provided better survival benefits compared to PMCT. However, there was a disparity in prognostic factors, suggesting evaluation of the two nomograms may be supportive in modality selection. Further prospective validation studies are required for the results to be applied in clinical medicine.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32156721

RESUMO

BACKGROUND: We aimed to evaluate the utility of p16/Ki-67 dual-stained cytology for triaging HPV-positive women. METHODS: HPV-positive women aged ≥ 21 years were recruited in a multicenter prospective observational study between May 2016 and May 2017. The clinical performance of dual-stained cytology, with or without HPV16/18 genotyping, were evaluated for all HPV-positive women to detect cervical intraepithelial neoplasia grade 2 or worse (CIN2+). RESULTS: Eight hundred and forty-six HPV-positive women aged ≥ 21 years with valid cervical biopsies were enrolled for this study. For CIN2+ detection, dual-stained cytology showed statistically higher specificity (85.28%) than Pap cytology (80.00%, P<0.001) and HPV16/18 genotyping (72.36%, P<0.001), while the sensitivity of dual-stained cytology (63.49%) remained comparable to that of Pap cytology (61.90%, P=0.832) and HPV16/18 genotyping (61.90%, P=0.897). HPV16/18 genotyping in combination with dual-stained cytology was more specific (62.50% vs. 58.06%, P<0.001), while showed similar sensitivity (86.51% vs. 85.71%, P=1.000), as compared to HPV16/18 genotyping in combination with Pap cytology. Similar patterns were also observed for CIN3+. CONCLUSIONS: p16/Ki-67 dual-stained cytology, either alone or in combination with HPV16/18 genotyping, showed a good stratification with high specificity and comparable sensitivity for HPV-positive women. IMPACT: This is one of the few studies that has evaluated the performance of dual-stained cytology for triaging HPV-positive women in China. The higher specificity and comparable sensitivity of dual-stained cytology in comparison to Pap cytology in the detection of CIN2+ or CIN3+ is of vital importance to developing countries, where Pap cytology faces many challenges.

4.
J Phys Chem Lett ; : 1411-1418, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32027143

RESUMO

Substantial progress has been achieved in red and green perovskite light-emitting diodes (PeLEDs). However, blue PeLEDs are still inferior in light-emitting efficiency and luminance compared with their green and red counterparts. Herein efficient blue PeLEDs simultaneously achieving high luminance and high color stability are fabricated based on the polycrystalline perovskites with a 3D Rb-Cs alloyed scaffold. The synergistic manipulation of an isopropanol antisolvent treatment and the PEDOT:PSS/blue perovskite interface modification with RbCl effectively improve the photoluminescence properties of the resultant blue polycrystalline 3D perovskite films and the final electroluminescence performance of the blue PeLEDs. The optimized blue PeLEDs show a maximum external quantum efficiency of 1.66% with an emission peak at 484 nm and a full width at half-maximum of 18 nm as well as CIE coordinates of (0.08, 0.21). Moreover, the optimized blue PeLEDs not only show superior color stability under various luminances but also achieve high luminances. The obtained maximum luminance of 9243 cd m-2 is one of the highest values among the efficient and color-stable blue PeLEDs.

5.
Environ Sci Pollut Res Int ; 27(10): 11174-11183, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31960242

RESUMO

The unique hydrogeological conditions of karst area make the groundwater react rapidly to rainfall events, which makes the groundwater more susceptible to anthropogenic pollutions. The current study based on a combined excitation-emission matrix fluorescence spectroscopy and parallel factor analysis (EEM-PARAFAC) and geochemical-statistical investigation of water samples from the karst water system in Xintian County, Hunan Province, China, gives crucial information about the principal factors influencing karst water hydrochemistry and dissolved organic matter (DOM). The analyzed data revealed that both surface water and descending spring samples were within the Ca-Mg-HCO3 water type and dominated by humic-like fluorophore, and well water samples were within both the Ca-Mg-HCO3 and Na-HCO3 water types and controlled by protein-like fluorophore. The chemical compositions of surface water and descending springs were mainly influenced by the weathering of silicate, carbonate, and evaporate rocks and precipitation. In addition to be affected by the weathering of silicate, carbonate, and evaporate rocks and precipitation, the well water was also impacted by ion exchange and other activities like anthropogenic. The DOM in the karst water system was affected by allochthonous and autochthonous inputs as well as the chemical compositions of the water.

6.
Cancer Med ; 9(4): 1544-1552, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31899608

RESUMO

As a prevalent tumor among women, breast cancer is still an incurable disease due to drug resistance. In this study, we report microRNA-221 to have a significant effect on breast cancer resistance to adriamycin. The microRNA-221 is elevated in tumor tissue compared with nearby nontumor samples, as well as in breast cancer cell line with adriamycin resistance (MCF-7/ADR) compared to its parental line (MCF-7) and the normal breast epithelial cell line (MCF-10A). Enforced level of microRNA-221 promotes cancer resistance to adriamycin, which in turn sustains cell survival and exacerbates malignant formation. Reciprocally, the silence of microRNA-221 in cancer cells augments the sensitivity to chemotherapy, thereby resulting in enhanced apoptosis of MCF-7/ADR cells. Mechanistically, we identify PTEN as a direct target of microRNA-221, which was conversely associated with a microRNA-221 level in breast tumors. The knock-down of PTEN partially reversed the stimulatory role of microRNA-221 in the modulation of the Akt/mTOR signaling. Taken together, these findings suggest microRNA-221 suppresses PTEN transcription and activates Akt/mTOR pathway, which in turn enhances breast cancer resistance to adriamycin and promotes cancer development. Our data thus illuminate the microRNA-221/PTEN axis may act as a promising strategy for the treatment of chemotherapy-resistant breast tumors.

7.
Life Sci ; 244: 117281, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31926249

RESUMO

AIMS: Mast cells play a crucial role in gastrointestinal physiology and pathophysiology. This study was conducted to investigate the role of mast cells (MCs) in the protective effect of Lactobacillus casei ATCC 393 (L. casei ATCC 393) on intestinal barrier function. MAIN METHODS: The regulatory effect of L. casei ATCC 393 on intestinal barrier dysfunction and MCs activation induced by Enterotoxigenic Escherichia coli K88 (ETEC K88) were evaluated by porcine mucosal mast cells (PMMCs)-porcine jejunal epithelial cells (IPEC-J2)-L. casei ATCC 393 co-culture experiments in vitro and MCs stabilizer drug experiment in vivo. KEY FINDINGS: Results showed that L. casei ATCC 393 pretreatment effectively alleviated the reduction of cell viability and increase of permeability in ETEC K88-infected IPEC-J2 cells. L. casei ATCC 393 pretreatment inhibited the increase of proinflammatory cytokines and some other MCs mediators, and decrease of anti-inflammatory cytokines in ETEC K88-infected PMMCs. Cromolyn sodium or L. casei ATCC 393 prevented ETEC K88-induced increase of intestinal epithelial cell permeability in IPEC-J2 cells when co-cultivation with PMMCs. Furthermore, cromolyn sodium or L. casei ATCC 393 pretreatment attenuated ETEC K88-induced increase of MCs mediators, mast cell proteases (MCPs) and carboxypeptidase A3 (CPA3) mRNA levels, and down-regulation of tight junction proteins, Toll-like receptor 2 and 4 (TLR2 and TLR4) expression levels in mice challenged by ETEC K88. SIGNIFICANCE: These results indicated that intestinal barrier dysfunction caused by ETEC K88 was mediated by intestinal mast cell activation which can be prevented by L. casei ATCC 393 via TLRs signaling pathway.


Assuntos
Mucosa Intestinal/metabolismo , Lactobacillus casei/metabolismo , Mastócitos/fisiologia , Animais , Linhagem Celular , Sobrevivência Celular , Escherichia coli Enterotoxigênica/metabolismo , Escherichia coli Enterotoxigênica/patogenicidade , Células Epiteliais/metabolismo , Infecções por Escherichia coli/metabolismo , Gastroenteropatias/metabolismo , Enteropatias/metabolismo , Intestinos/fisiologia , Masculino , Mastócitos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Modelos Animais , Suínos , Receptores Toll-Like/metabolismo
8.
Chemosphere ; 243: 125329, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31751926

RESUMO

To better understand the relationships between soil pore structure features and soil CO2 emission and soil organic carbon (SOC) sequestration following different straw return modes, undisturbed soil cores (0-5 cm and 5-10 cm) were collected from a rice-wheat rotation system under 4 straw return treatments as (1) no straw return (CK), (2) straw direct return (DR), (3) straw biochar return (BR); (4) straw-pig manure fermentation return (FR) for six years. Pore structure parameters including pore size distribution, porosity, connectivity, anisotropy and fractal dimension (FD) were determined using X-ray computer tomography. Soil CO2 flux and concentrations of SOC, readily oxidable carbon and nutrients were also measured. The results showed that BR and FR had significantly higher SOC concentration than DR and CK. Porosity and number of >500 µm and 500-100 µm macropores, FD and connectivity were significantly highest under FR and was lowest under BR. FR and DR produced 28.1%-32.4% higher C-CO2 than CK and BR in wheat growing season, and 9.80%-16.9% higher in rice season. Soil CO2 emission and C concentrations were significantly related to soil pore structure parameters. The CO2 emission was most significantly related to number of >500 µm pores and FD, indicating that poorly developed pore structure under BR hindered the production and diffusion of CO2 from soil. These results enhanced our understanding of the relationship between soil pore structure and CO2 emission following biochar application, and provided evidence for decision making process in choosing proper straw managements to promote SOC sequestration and reduce CO2 emission.

9.
Hepatology ; 71(1): 130-147, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31148183

RESUMO

Hepatocellular carcinoma (HCC) is one of the fastest-rising causes of cancer-related death worldwide, but its deficiency of specific biomarkers and therapeutic targets in the early stages lead to severe inadequacy in the early diagnosis and treatment of HCC. Covalently closed circular RNA (circRNA), which was once considered an aberrant splicing by-product, is now drawing new interest in cancer research because of its remarkable functionality. Beneath the surface of the dominant functional proteins events, a hidden circRNA-centric noncoding regulatory RNAs network active in the very early stage of HCC is here revealed by a genome-wide analysis of mRNA, circRNA, and microRNA (miRNA) expression profiles. Circ-CDYL (chromodomain Y like) is specifically up-regulated in the early stages of HCC and therefore contributes to the properties of epithelial cell adhesion molecule (EPCAM)-positive liver tumor-initiating cells. Circ-CDYL interacts with mRNAs encoding hepatoma-derived growth factor (HDGF) and hypoxia-inducible factor asparagine hydroxylase (HIF1AN) by acting as the sponge of miR-892a and miR-328-3p, respectively. Subsequently, activation of the phosphoinositide 3-kinase (PI3K)-AKT serine/threonine kinase-mechanistic target of rapamycin kinase complex 1/ß-catenin and NOTCH2 pathways, which promote the expression of the effect proteins, baculoviral IAP repeat containing 5 (BIRC5 or SURVIVIN) and MYC proto-oncogene, is influenced by circ-CDYL. A treatment incorporating circ-CDYL interference and traditional enzyme inhibitors targeting PI3K and HIF1AN demonstrated highly effective inhibition of stem-like characteristics and tumor growth in HCC. Finally, we demonstrated that circ-CDYL expression or which combined with HDGF and HIF1AN are both independent markers for discrimination of early stages of HCC with the odds ratios of 1.09 (95% confidence interval [CI], 1.02-1.17) and 124.58 (95% CI, 13.26-1170.56), respectively. Conclusion: These findings uncover a circRNA-centric noncoding regulatory RNAs network in the early stages of HCC and thus provide a possibility for surveillance and early treatment of HCC.

10.
J Biol Chem ; 294(46): 17471-17486, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594861

RESUMO

Constitutive activation of signal transducer and activator of transcription 3 (STAT3) occurs in ∼70% of human cancers, and STAT3 is regarded as one of the most promising targets for cancer therapy. However, specific direct STAT3 inhibitors remain to be developed. Oridonin is an ent-kaurane plant-derived diterpenoid with anti-cancer and anti-inflammatory activities. Here, using an array of cell-based and biochemical approaches, including cell proliferation and apoptosis assays, pulldown and reporter gene assays, site-directed mutagenesis, and molecular dynamics analyses, we report that a thiazole-derived oridonin analogue, CYD0618, potently and directly inhibits STAT3. We found that CYD0618 covalently binds to Cys-542 in STAT3 and suppresses its activity through an allosteric effect, effectively reducing STAT3 dimerization and nuclear translocation, as well as decreasing expression of STAT3-targeted oncogenes. Remarkably, CYD0618 not only strongly inhibited growth of multiple cancer cell lines that harbor constitutive STAT3 activation, but it also suppressed in vivo tumor growth via STAT3 inhibition. Taken together, our findings suggest Cys-542 as a druggable site for selectively inhibiting STAT3 and indicate that CYD0618 represents a promising lead compound for developing therapeutic agents against STAT3-driven diseases.

11.
Int J Nanomedicine ; 14: 4491-4502, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31417254

RESUMO

Background: Selenium (Se) can exert antioxidative activity and prevent the body from experiencing oxidative injury. Biogenic Se nanoparticles (SeNPs) synthesized by probiotics possess relatively strong chemical stability, high bioavailability, and low toxicity, this makes them potential Se supplements. Previously, we demonstrated that SeNPs synthesized by Lactobacillus casei ATCC 393 can alleviate hydrogen peroxide (H2O2)-induced human and porcine intestinal epithelial cells' oxidative damage. However, the antioxidant mechanism remains unclear. Methods: The possible antioxidant mechanism and protective effect of SeNPs on intestinal epithelial permeability and mitochondrial function were evaluated by establishing an H2O2-induced oxidative damage model of human colon mucosal epithelial cells (NCM460) and conducting Nrf2 inhibitor interference experiments. Mitochondrial membrane potential (MMP), mitochondrial DNA content, adenosine triphosphate (ATP), ROS, and protein expression levels of Nrf2-related genes were determined. Mitochondrial ultrastructure was visualized by transmission electron microscopy. Results: An amount of 4 µg Se/mL of SeNPs synthesized by L. casei ATCC 393 alleviated increase of ROS, reduced ATP and MMP, and maintained intestinal epithelial permeability in NCM460 cells challenged by H2O2. In addition, SeNPs improved the protein levels of Nrf2, HO-1, and NQO-1. Moreover, SeNPs attenuated the damage of mitochondrial ultrastructure caused by oxidative stress. Nrf2 inhibitor (ML385) abolished the regulatory effect of SeNPs on intracellular ROS production. Conclusion: Data suggest that biogenic SeNPs synthesized by L. casei ATCC 393 can protect the intestinal epithelial barrier function against oxidative damage by alleviating ROS-mediated mitochondrial dysfunction via Nrf2 signaling pathway. Biogenic SeNPs are an attractive candidate for potential Se supplement agent in preventing oxidative stress-related intestinal disease by targeting mitochondria.


Assuntos
Mucosa Intestinal/patologia , Mucosa Intestinal/fisiopatologia , Lactobacillus casei/metabolismo , Mitocôndrias/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Nanopartículas/química , Estresse Oxidativo/efeitos dos fármacos , Selênio/farmacologia , Animais , Antioxidantes/farmacologia , Linhagem Celular , Permeabilidade da Membrana Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/toxicidade , Mucosa Intestinal/efeitos dos fármacos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/ultraestrutura , Modelos Biológicos , Nanopartículas/ultraestrutura , Oxirredução , Substâncias Protetoras/farmacologia , Transdução de Sinais/efeitos dos fármacos , Suínos
12.
Front Microbiol ; 10: 1632, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31402902

RESUMO

Lactococcus lactis (L. lactis) NZ9000, which has been genetically modified, is the most commonly used host strain for nisin regulated gene expression. Selenium (Se) is an essential trace element in the diet of humans and animals important for the maintenance of health and growth. Biosynthesized Se nanoparticles (SeNPs) that use microorganisms as a vehicle are uniquely advantages in terms of low costs, low toxicity and high bioavailability. This study was aimed at preparing novel functionalized SeNPs by L. lactis NZ9000 through eco-friendly and economic biotechnology methods. Moreover, its physicochemical characteristics, antioxidant and anti-inflammatory activities were investigated. L. lactis NZ9000 synthesized elemental red SeNPs when co-cultivated with sodium selenite under anaerobic conditions. Biosynthesized SeNPs by L. lactis NZ9000 were mainly capped with polysaccharides and significantly alleviated the increase of malondialdehyde (MDA) concentration, the decrease of glutathione peroxidase (GPx) and total superoxide dismutase (T-SOD) activity in porcine intestinal epithelial cells (IPEC-J2) challenged by hydrogen peroxide (H2O2). SeNPs also prevented the H2O2-caused reduction of transepithelial electrical resistance (TEER) and the increase of FITC-Dextran fluxes across IPEC-J2. Moreover, SeNPs attenuated the increase of reactive oxygen species (ROS), the reduction of adenosine triphosphate (ATP) and the mitochondrial membrane potential (MMP) and maintained intestinal epithelial permeability in IPEC-J2 cells exposed to H2O2. In addition, SeNPs pretreatment alleviated the cytotoxicity of Enterotoxigenic Escherichia coli (ETEC) K88 on IPEC-J2 cells and maintained the intestinal epithelial barrier integrity by up-regulating the expression of Occludin and Claudin-1 and modulating inflammatory cytokines. Biosynthesized SeNPs by L. lactis NZ9000 are a promising selenium supplement with antioxidant and anti-inflammatory activities.

13.
ChemSusChem ; 12(18): 4202-4210, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31328347

RESUMO

Sustainable, inexpensive, and environmentally friendly biomass waste can be exploited for large-scale production of carbon nanomaterials. Here, alkali lignin was employed as a precursor to synthesize carbon quantum dots (CQDs) with bright green fluorescence through a simple one-pot route. The prepared CQDs had a size of 1.5-3.5 nm, were water-dispersible, and showed wonderful biocompatibility, in addition to their excellent photoluminescence and electrocatalysis properties. These high-quality CQDs could be used in a wide range of applications such as metal-ion detection, cell imaging, and electrocatalysis. The wide range of biomass lignin feedstocks provide a green, low-cost, and viable strategy for producing high-quality fluorescent CQDs and enable the conversion of biomass waste into high-value products that promote sustainable development of the economy and human society.

14.
J Food Sci ; 84(5): 1054-1059, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31042817

RESUMO

This study was designed to determine the effects of µ/m-calpain on the degradation of cytoskeletal proteins in pectoralis major. Four chickens were slaughtered and the breasts were removed and stored for 12 hr at 4 °C. Each sample was divided into three groups and respectively immersed in control reagent, calpain inhibitor, and caspase inhibitor at 4 °C. The samples were used to evaluate troponin-T and desmin degradation, calpain activity, and myofibril ultrastructure at 12 hr, day 1, day 3, and day 7. Casein zymography revealed that µ-calpain could not be detected in all samples after 12 hr postmortem. The calpain inhibitor inhibited µ/m-calpain activity and reduced troponin-T and desmin degradation during 7 day postmortem. The caspase inhibitor inhibited µ/m-calpain activity and, troponin-T and desmin degradation before day 3 postmortem. The findings suggest that, µ/m-calpain had an effect on cytoskeletal protein degradation after 12 hr postmortem.


Assuntos
Calpaína , Carne/análise , Proteínas Musculares , Animais , Calpaína/antagonistas & inibidores , Calpaína/metabolismo , Galinhas , Proteínas Musculares/química , Proteínas Musculares/metabolismo , Músculo Esquelético/química , Músculo Esquelético/metabolismo
15.
Appl Opt ; 58(12): 3277-3281, 2019 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-31044804

RESUMO

Laser-induced breakdown spectroscopy (LIBS) coupled with the linear multivariate calibration method was applied to analyze nitrogen (N) quantitatively in ammonium phosphate fertilizers. The intensity of lines N (NI:742.4, 744.2, 746.8, 856.7, 859.4, 862.9, 870.3, 871.2, 871.8 nm) and O (OI:777.2, 844.6, 882.0 nm) were used as independent variables for the models. To verify the accuracy of the models, the unary, binary, ternary, and quaternary variables were chosen to establish the linear regression equations. The results of the linear models showed that the quaternary model was better than the other three models. The correlation coefficient of the quaternary linear model was 0.981 and the maximum relative error of the validation samples was 4.32%.

16.
Front Chem ; 7: 306, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31134183

RESUMO

Currently, exploring the applications of intermolecular donor-acceptor exciplex couple as host of OLEDs with phosphorescence, thermally activated delayed fluorescence (TADF) or fluorescence emitter as dopant is a hot topic. Compared to other host strategies, interfacial exciplex has the advantage in various aspects, such as barrier-free charge injection, unimpeded charge transport, and the energy-saving direct exciton formation process at the "Well"-like heterojunction interface region. Most importantly, due to a very fast and efficient reverse intersystem-crossing (RISC) process, such a host is capable of regulating singlet/triplet exciton populations in itself as well as in the dopant emitters both under photoluminescent (PL) and electroluminescent (EL) driving conditions. In this mini-review, we briefly summarize and comment on recent applications of this ideal host in OLEDs (including both thermal-evaporation OLEDs and solution-processed OLEDs) with diverse emitters, e.g., fluorescence, phosphorescence, delayed fluorescence, or others. Special attention is given to illustrate the peculiar achievement of high overall EL performance with superiorities of low driving voltages, slow roll-off rate, high power efficiencies and satisfied device lifetime using this host strategy, which is then concluded by personal perspectives on the relevant next-step in this field.

17.
ACS Appl Mater Interfaces ; 11(18): 16720-16728, 2019 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-30983318

RESUMO

Exploring efficient bifunctional oxygen electrocatalysts is beneficial to promote the practical applications for rechargeable Zn-air batteries. Herein, a high-efficiency one-pot method is developed to synthesize porous carbon with N, S doping and embedded hollow cobalt oxide nanoparticles. The coordination of polyethyleneimine molecules with cobalt ions enables the formation of organic-inorganic precursors via the co-precipitation with lignosulfonate because of the electrostatic interaction. Under thermal treatment, the hollow cobalt oxide nanoparticles can be well dispersed among the carbon matrix codoped with N, S. The as-prepared composite catalysts exhibit efficient bifunctional activity for electrochemical reduction and evolution reactions of oxygen, thanks to the N, S codoping nature and the hollow cobalt oxide with abundant oxygen vacancies. The bifunctional catalytic activity renders the assembly of high-performance Zn-air battery in an aqueous electrolyte with a specific capacity of 745 mA h gZn-1 and good cycling stability for over 100 h. More importantly, the all-solid-state Zn-air battery is assembled with a polymer-based electrolyte, also exhibiting good cycling stability and flexibility under various bending status.

18.
Sensors (Basel) ; 19(7)2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30978922

RESUMO

Rapid detection of phosphorus (P) element is beneficial to the control of compound fertilizer production process and is of great significance in the fertilizer industry. The aim of this work was to compare the univariate and multivariate analysis of phosphorus element in compound fertilizers and obtain a reliable and accurate method for rapid detection of phosphorus element. A total of 47 fertilizer samples were collected from the production line; 36 samples were used as a calibration set, and 11 samples were used as a prediction set. The univariate calibration curve was constructed by the intensity of characteristic line and the concentration of P. The linear correlation coefficient was 0.854 as the existence of the matrix effect. In order to eliminate the matrix effect, the internal standardization as the appropriate methodology was used to increase the accuracy. Using silicon (Si) element as an internal element, a linear correlation coefficient of 0.932 was obtained. Furthermore, the chemometrics model of partial least-squares regression (PLSR) was used to analysis the concentration of P in fertilizer. The correlation coefficient was 0.977 and 0.976 for the calibration set and prediction set, respectively. The results indicated that the LIBS technique coupled with PLSR could be a reliable and accurate method in the quantitative determination of P element in complex matrices like compound fertilizers.

19.
Gene ; 694: 102-110, 2019 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-30716440

RESUMO

Increasing evidence has demonstrated that long non-coding RNAs (lncRNAs) contribute to tumorigenesis, progression and recurrence of various malignancies including Gallbladder carcinoma (GBC). Lnc-DILC is reported to be the tumor suppressor gene to play an important role in liver cancer stem cells (CSCs). However, the role of lnc-DILC in GBC remains to be elucidated. Herein, we show that lnc-DILC is upregulated in gallbladder CSCs and GBC patients' tissues. Knockdown of lnc-DILC attenuates the self-renewal, tumorigenicity, proliferation and metastasis of gallbladder CSCs. Mechanistically, lnc-DILC promotes gallbladder CSCs expansion via Wnt/ß-catenin pathway. Special Wnt/ß-catenin inhibitor FH535 diminishes the discrepancy of self-renewal, growth and metastasis between lnc-DILC interference GBC cells and their control cells. In conclusion, lnc-DILC drives gallbladder CSCs self-renewal, tumorigenicity, proliferation and metastasis by activating Wnt/ß-catenin signaling, and may therefore prove to be a potential therapeutic target for GBC patients.


Assuntos
Neoplasias da Vesícula Biliar/genética , RNA Longo não Codificante/genética , Adulto , Animais , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células/genética , Transformação Celular Neoplásica/genética , China , Progressão da Doença , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Camundongos , Camundongos Nus , Recidiva Local de Neoplasia/genética , Células-Tronco Neoplásicas/metabolismo , RNA Longo não Codificante/metabolismo , Transdução de Sinais/genética , Via de Sinalização Wnt/genética , Ensaios Antitumorais Modelo de Xenoenxerto/métodos
20.
Sensors (Basel) ; 19(3)2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30717147

RESUMO

Automatic recognition of ripening tomatoes is a main hurdle precluding the replacement of manual labour by robotic harvesting. In this paper, we present a novel automatic algorithm for recognition of ripening tomatoes using an improved method that combines multiple features, feature analysis and selection, a weighted relevance vector machine (RVM) classifier, and a bi-layer classification strategy. The algorithm operates using a two-layer strategy. The first-layer classification strategy aims to identify tomato-containing regions in images using the colour difference information. The second classification strategy is based on a classifier that is trained on multi-medium features. In our proposed algorithm, to simplify the calculation and to improve the recognition efficiency, the processed images are divided into 9 × 9 pixel blocks, and these blocks, rather than single pixels, are considered as the basic units in the classification task. Six colour-related features, namely the Red (R), Green (G), Blue (B), Hue (H), Saturation (S) and Intensity (I) components, respectively, colour components, and five textural features (entropy, energy, correlation, inertial moment and local smoothing) were extracted from pixel blocks. Relevant features and their weights were analysed using the iterative RELIEF (I-RELIEF) algorithm. The image blocks were classified into different categories using a weighted RVM classifier based on the selected relevant features. The final results of tomato recognition were determined by combining the block classification results and the bi-layer classification strategy. The algorithm demonstrated the detection accuracy of 94.90% on 120 images, this suggests that the proposed algorithm is effective and suitable for tomato detection.

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