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2.
J Infect Dev Ctries ; 15(4): 490-500, 2021 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-33956648

RESUMO

INTRODUCTION: Nutritional status has been defined as an individual's health condition. The relationship between the progression of COVID-19 and Nutritional status is still unclear. We analyzed the clinical characteristics of 342 coronavirus disease 2019 (COVID-19) patients, and analyzed the relationship between the progression of COVID-19 and Nutritional status. METHODOLOGY: 342 COVID-19 were enrolled from ten different hospitals in China. The clinical characteristics were collected and analyzed. RESULTS: The body mass index (BMI) of the mild patients (Group A) was higher than those in severe patients (Group B) and critical patients (Group C); The lactate dehydrogenase (LDH) level of Group A was lower than those of the other two groups; Sex, age, and BMI, was strongly correlated with Clinical classification (CT); Among the laboratory test results, Neutrophil (NEU%), Aspartate aminotransferase (AST), LDH, and blood glucose (BG) were positively correlated with CT; Lymphocyte ( LYM%), Platelet (PLT), Albumin (ALB), and Creatinine (Cr) were negatively correlated with CT. BMI, NEU%, LYM%, ALB, Cr, and PLT are all protective factors that affect CT. CONCLUSION: People with poor nutritional status (lower BMI and ALB) have a higher risk of developing severe disease after infection with SARS-CoV-2. In the clinical treatment of COVID-19, individualized nutritional support is very important for the rehabilitation of patients.

3.
J Thorac Oncol ; 2021 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-33915252

RESUMO

INTRODUCTION: ZL-2306-005 is a randomized, double-blind, multicenter phase 3 study evaluating the efficacy and safety of niraparib, a poly(ADP-ribose) polymerase inhibitor, as first-line maintenance therapy in Chinese patients with platinum-responsive, extensive-stage small cell lung cancer (ES-SCLC). METHODS: Patients with complete/partial response (CR/PR) to standardized, platinum-based first-line chemotherapy were randomized 2:1 to receive niraparib or placebo (300 mg [baseline body weight ≥77 kg, platelet count ≥150,000/µL] or 200 mg) once daily until progression or unacceptable toxicity. Primary endpoints were progression-free survival (PFS) (blinded independent central review, BICR) and overall survival (OS) (sample size planned: 591 patients). Secondary endpoints included investigator-assessed PFS and safety. RESULTS: ZL-2306-005 was terminated early due to ES-SCLC treatment landscape changes (data cut-off: 20 Mar 2020). During July 2018-February 2020, 185 of 272 patients screened were randomized (niraparib: n=125 [CR=1, PR=124]; placebo: n=60 [CR=1, PR=59]). Median (95% confidence interval [CI]) PFS (BICR) was 1.54 months (1.41-2.69, niraparib) and 1.36 months (1.31-1.48, placebo); hazard ratio [HR]=0.66 (95% CI: 0.46-0.95; p=0.0242). Median OS was 9.92 months (9.33-13.54, niraparib) and 11.43 months (9.53-not estimable, placebo); HR=1.03 (95% CI: 0.62-1.73; p=0.9052). Median investigator-assessed PFS was 1.48 months (1.41-2.56, niraparib) and 1.41 months (1.31-2.00, placebo); HR=0.88 (95% CI: 0.61-1.26; p=0.4653). Grade ≥3 adverse events occurred in 34.4% (niraparib) and 25.0% (placebo) of patients. CONCLUSIONS: ZL-2306-005 did not reach primary endpoints. However, niraparib as maintenance therapy modestly improved PFS in patients with platinum-responsive ES-SCLC, with acceptable tolerability profile and no new safety signal.

4.
Artigo em Inglês | MEDLINE | ID: mdl-33811023

RESUMO

Bacteriocins have attracted increasing interest because of their potential as natural preservatives. Recent studies showed that the Bacillus cereus group is a prominent producer of bacteriocins. Using a laboratory-based screening strategy, we identified a strain in the B. cereus group, B. toyonensis XIN-YC13, with antimicrobial activity against B. cereus A novel, 70-amino acid-long leaderless bacteriocin, toyoncin, was purified from the culture supernatant of strain XIN-YC13 and its molecular mass was found to be 7817.1012 Da. Toyoncin shares no similarity with any other known bacteriocins, and its N-terminal amino acid is formylmethionine rather than methionine. Toyoncin shows good pH and heat stability and exhibits specific antimicrobial activity against two important food-borne pathogens, B. cereus and Listeria monocytogenes Additionally, toyoncin exerts bactericidal activity and induces cell membrane damage. Toyoncin can also inhibit the outgrowth of B. cereus spores. The preservation assays showed that toyoncin effectively suppressed or eradicated B. cereus and L. monocytogenes in pasteurized skim milk. These results suggest that toyoncin can be used as a new bio-preservative against B. cereus and L. monocytogenes in the food industry.Importance:We identified a novel leaderless bacteriocin, toyoncin, produced by B. toyonensis XIN-YC13. Toyoncin shows good pH and heat stability; has specific antimicrobial activity against, B. cereus and L. monocytogenes (two important food-borne pathogens), and destroys their cell membrane integrity. Toyoncin inhibited the outgrowth of B. cereus spores and effectively inhibited or eliminated B. cereus and L. monocytogenes in a milk model system. These results indicate the potential of toyoncin as a food preservative.

5.
Environ Pollut ; 282: 117031, 2021 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-33831629

RESUMO

In this study, 24 surface water samples were collected from Dongting Lake, China, in the wet and dry seasons, then the concentrations, composition profiles and spatio-seasonal variations of nine organophosphate triesters (OPEs) and five organophosphate diesters (Di-OPs) were determined. Significantly higher total OPE concentrations (∑OPEs) were observed in the wet season (49.5-148 ng L-1) than in the dry season (5.00-45.7 ng L-1) suggesting higher input via tributaries discharge as well as wet deposition in the studied region. Whereas lower levels of TnBP and (triphenyl phosphate (TPHP) in wet season reflected their possible degradation under solar irradiation. Comparable levels of total Di-OPs (∑Di-OPs) were found in the wet season (3.41-13.9 ng L-1) and dry season (1.01-12.3 ng L-1). Tri(2-chloroethyl) phosphate and tris(2-chloroisopropyl) phosphate were the main OPE components, while diphenyl phosphate, di-n-butyl phosphate and bis(1,3-dichloro-2-propyl) phosphate were the main Di-OP components. High levels of OPEs and Di-OPs were found in Datong Lake suggesting possible local emissions potentially related to fishery activity in the land-locked lake. Samples at river mouths to the lake also have higher levels of target OPEs and Di-OPs, the results disclosed obvious discharges from tributaries in Hunan Province. Negligible non-carcinogenic and carcinogenic risks were determined based on the measured concentrations in source waters. A limited ecological risk aquatic organisms in the Dongting Lake was identified, with most risk from TPHP.

6.
Blood ; 2021 02 24.
Artigo em Inglês | MEDLINE | ID: mdl-33881475

RESUMO

Total body irradiation (TBI) is commonly used in host conditioning regimens for human hematopoietic stem cell (HSC) transplantation to treat various hematological disorders. Exposure to TBI not only induces acute myelosuppression and immunosuppression but also impairs the various components of the HSC niche in recipients. Our previous study demonstrated that radiation-induced bystander effects (RIBE) of irradiated recipients decreased the long-term repopulating ability of transplanted mouse HSCs. However, RIBE on human HSCs have not been studied. Here, we report that RIBE on transplanted human hematopoietic cells impaired the long-term hematopoietic reconstitution of human HSCs as well as the colony-forming ability of human hematopoietic progenitor cells (HPCs). Our further studies found that the RIBE-affected human hematopoietic cells showed enhanced DNA damage responses, cell cycle arrest and p53-dependent apoptosis, mainly due to oxidative stress. Moreover, multiple antioxidants could mitigate these bystander effects, though at different efficacies both in vitro and in vivo. Taken together, these findings suggest that RIBE impairs human HSCs by oxidative DNA damage. This study provides definitive evidence for RIBE in transplanted human HSCs and further justifies the necessity for conducting clinical trials to assess the ability of multiple antioxidants to improve the efficacy of HSC transplantation for patients with hematological or non-hematological disorders.

7.
Hum Brain Mapp ; 2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33739571

RESUMO

Network neuroscience has broadly conceptualized the functions of the brain as complex communication within and between large-scale neural networks. Nevertheless, whether and how the gut microbiota influence functional network connectivity that in turn impact human behaviors has yet to be determined. We collected fecal samples from 157 healthy young adults and used 16S sequencing to assess gut microbial diversity and enterotypes. Large-scale inter- and intranetwork functional connectivity was measured using a combination of resting-state functional MRI data and independent component analysis. Sleep quality and core executive functions were also evaluated. Then, we tested for potential associations between gut microbiota, functional network connectivity and behaviors. We found significant associations of gut microbial diversity with internetwork functional connectivity between the executive control, default mode and sensorimotor systems, and intranetwork connectivity of the executive control system. Moreover, some internetwork functional connectivity mediated the relations of microbial diversity with sleep quality, working memory, and attention. In addition, there was a significant effect of enterotypes on intranetwork connectivity of the executive control system, which could mediate the link between enterotypes and executive function. Our findings not only may expand existing biological knowledge of the gut microbiota-brain-behavior relationships from the perspective of large-scale functional network organization, but also may ultimately inform a translational conceptualization of how to improve sleep quality and executive functions through the regulation of gut microbiota.

8.
Toxicol Mech Methods ; : 1-12, 2021 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-33749501

RESUMO

Crotonaldehyde is a highly toxic pollutant, widely present in tobacco smoke and automobile exhaust. Exposure to crotonaldehyde can cause hepatotoxicity and induce liver tumors in rats; however, the underlying mechanism is unclear. Liver cells contain many mitochondria, which serve to maintain energy levels in the body. We hypothesized that the energy metabolism disorder caused by mitochondrial dysfunction is an important cause of liver injury in rats exposed to crotonaldehyde. To test this, we randomly divided 40 male Wistar rats into four groups, and provided crotonaldehyde at 0, 2.5, 4.5, and 8.5 mg/kg for 90 days by intragastric administration. The results showed that crotonaldehyde exposure caused damage to liver mitochondrial structure, reduced electron-transport chain activity and ATP levels, and interfered with mitochondrial DNA transcription. In response to increased crotonaldehyde exposure, rats exhibited increased reactive oxygen species levels, decreased superoxide dismutase and glutathione activity, and activation of the caspase-mediated apoptosis pathway, as well as elevated levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, and histopathological damage. Our findings, together with those of previous reports, should help elucidate the underlying mechanism of crotonaldehyde-induced mitochondrial dysfunction and energy metabolism disorder, and provide an important direction for the prevention and clinical intervention of liver diseases caused by crotonaldehyde and aldehydes with similar structures.

9.
Org Lett ; 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33769063

RESUMO

An unprecedented method for the construction of thiazole-2-thiones via an oxidative cascade cyclization strategy is described. The novel protocol involves the simultaneous formation of two C-S bonds and a C═S bond on the structure of enaminones in a single operation through a cascade of C(sp2)-H/C(sp3)-H bond sulfurations and C(sp3)-H bond thiocarbonylation. This transformation allows for the efficient synthesis of thiazole-2-thiones with broad tolerance in moderate to excellent yields from simple enaminones with elemental sulfur.

10.
Sci China Life Sci ; 2021 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-33564978

RESUMO

The blood and immune system of coronavirus disease 2019 (COVID-19) infected patients are dysfunctional, and numerous studies have been conducted to resolve their characteristics and pathogenic mechanisms. Nevertheless, the variations of immune responses along with disease severity have not been comprehensively documented. Here, we profiled the single-cell transcriptomes of 96,313 peripheral blood mononuclear cells (PBMCs) derived from 12 COVID-19 patients (including four moderate, four severe and four critical cases) and three healthy donors. We showed that proliferative CD8 effector T cells with declined immune functions and cytotoxicity accumulated in the critical stage. By contrast, the quantity of natural killer (NK) cells was significantly reduced, while they exhibited enhanced immune activities. Notably, a gradually attenuated responseto COVID-19 along with disease severity was observed in monocytes, in terms of cellular composition, transcriptional discrepancy and transcription factor regulatory network. Furthermore, we identified immune cell-type dependent cytokine signatures distinguishing the severity of COVID-19 patients. In addition, cell interactions between CD8 effector T/NK cells and monocytes mediated by inflammatory cytokines were enhanced in moderate and severe stages, but weakened in critical cases. Collectively, our work uncovers the cellular and molecular players underlying the disordered and heterogeneous immune responses associated with COVID-19 severity, which could provide valuable insights for the treatment of critical COVID-19 patients.

11.
Org Lett ; 23(5): 1880-1885, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33595333

RESUMO

In this report, a P(NMe2)3-mediated reductive intramolecular annulation reaction has been developed with benzoyl formates bearing a trisubstituted alkene unit. It provides a facile synthesis of highly functionalized 2,2-disubstituted 2H-chromenes with a broad substrate scope and high efficiency. Experimental results suggest this annulation reaction proceeds via a cascade of alkene isomerization/vinyl o-quinone methide formation/6π-electrocyclization. As a key intermediate, the vinyl-substituted o-quinone methide is presumably generated by a Kukhtin-Ramirez adduct initiated O → C vinyl migration.

12.
Mol Immunol ; 132: 165-171, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33592572

RESUMO

The therapeutic options of non-small cell lung cancer (NSCLC) are limited, although a combination of targeted therapy and immunotherapy is promising. To explore novel targets for immunotherapy, we explored the role of Ccr4-Not transcription complex subunit 4 (CNOT4) in NSCLC. The expression of CNOT4 in tumor tissues was determined by immunohistochemistry staining and western blotting. The cell lines that stably express CNOT4 were established in H1299 and A549 cells. Direct cell counting, MTT assay, and colony formation were used to determine the ability of cell proliferation. Cell apoptosis and cell cycle were next analyzed by PI/Annexin V staining. Cell invasion and migration were examined by transwell assays. To further explore the function of CNOT4 in cytotoxic T lymphocytes (CTLs) mediated cytotoxicity, an in vitro co-culture system of CNOT4 overexpressing and control H1299 cells with CTLs was developed. CNOT4 was down-regulated in tumor tissues compared with paired normal tissues from patients with lung cancers. CNOT4 overexpression significantly inhibited tumor cell proliferation, colony formation, cell migration, and invasion, but promoted cell apoptosis. Furthermore, overexpression of CNOT4 enhanced cytotoxicity of CTLs to H1299. CNOT4 functions as a potential tumor suppressor of NSCLC via inhibiting tumor cell function and increasing the sensitivity to CTLs.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Neoplasias Pulmonares/metabolismo , Linfócitos T Citotóxicos/metabolismo , Fatores de Transcrição/metabolismo , Células A549 , Apoptose/fisiologia , Ciclo Celular/fisiologia , Linhagem Celular Tumoral , Movimento Celular/fisiologia , Proliferação de Células/fisiologia , Regulação para Baixo/fisiologia , Humanos , MicroRNAs/metabolismo
13.
Sci Total Environ ; 775: 145698, 2021 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-33631579

RESUMO

Karst groundwater is crucial, but particularly vulnerable to contaminants. Anthropologically derived pollutants on the surface-environment in karst areas could easily and rapidly enter groundwater through highly developed transmissible structures and threaten water safety. To investigate such transport, we analyzed 24 organochlorine pesticides (OCPs) in the multimedia environment from the Zigui karst area of China, where agriculture is the predominant human activity. OCPs were frequently detected with the total OCP concentrations ranged from 228 to 7970 pg/g, 300 to 32,200 pg/L, 318 to 2250 pg/L, 149 to 2760 pg/g, and 752 to 12,000 pg/g in the soil, spring water, river water, spring sediment, and river sediment, respectively. HCB and p,p'-DDT were the most dominant OCP species. Isomeric and metabolic ratios indicated fresh inputs of Lindane, technical DDT, and Aldrin, although they have been banned in China. The spatial distributions, correlation analysis, and regression analysis suggested rapid OCP transport from the soil to the spring water, and from the soil and spring water to river water. OCPs in the soil and springs explained 92.3% and 89.0% of those in the spring water and river water, respectively. The solid transport with the fast-moving water was predominant for OCPs in sediments. Highly dynamic water systems and rapid OCP transport in the intro- and inter-medium suggested by our results substantiate the groundwater's vulnerability in karst areas. More studies on levels and transport of organic contaminants in karst systems and policy for protecting the karst groundwater are urgently required to control contaminant sources and ensure groundwater sustainability, since the karst water resources may suffer a potentially bleak future consisted of the decreased groundwater quantity and low water quality.

14.
J Vis Exp ; (167)2021 01 23.
Artigo em Inglês | MEDLINE | ID: mdl-33554959

RESUMO

The morphology, size and quantity of cells, starch granules and protein bodies in seed determine the weight and quality of seed. They are significantly different among different regions of seed. In order to view the morphologies of cells, starch granules and protein bodies clearly, and quantitatively analyze their morphology parameters accurately, the whole-seed-sized section is needed. Though the whole-seed-sized paraffin section can investigate the accumulation of storage materials in seeds, it is very difficult to quantitatively analyze the morphology parameters of cells and storage materials due to the low resolution of the thick section. The thin resin section has high resolution, but the routine resin sectioning method is not suitable to prepare the whole-seed-sized section of mature seeds with a large volume and high starch content. In this study, we present a simple dry sectioning method for preparing the whole-seed-sized resin section. The technique can prepare the cross and longitudinal whole-seed-sized sections of developing, mature, germinated, and cooked seeds embedded in LR White resin, even for large seeds with high starch content. The whole-seed-sized section can be stained with fluorescent brightener 28, iodine, and Coomassie brilliant blue R250 to specifically exhibit the morphology of cells, starch granules, and protein bodies clearly, respectively. The image obtained can also be analyzed quantitatively to show the morphology parameters of cells, starch granules, and protein bodies in different regions of seed.


Assuntos
Microtomia/métodos , Resinas Sintéticas/química , Sementes/química , Zea mays/química , Proteínas de Plantas/metabolismo , Sementes/citologia , Coloração e Rotulagem , Amido/metabolismo , Zea mays/citologia , Zea mays/embriologia
15.
Nano Lett ; 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33599511

RESUMO

The rechargeable Zn-air batteries as an environmentally friendly sustainable energy technology have been extensively studied. However, it is still a challenge to develop non-noble metal bifunctional catalysts with high oxygen reduction as well as oxygen evolution reaction (ORR and OER) activity and superior durability, which limit the large-scale application of rechargeable Zn-air batteries. Herein, we synthesized an ultrastable FeCo bifunctional oxygen electrocatalyst on Se-doped CNTs (FeCo/Se-CNT) via a gravity guided chemical vapor deposition (CVD) strategy. The catalyst exhibits excellent ORR (E1/2 = 0.9 V) and OER (overpotential at 10 mA cm-2 = 340 mV) properties simultaneously, surpassing commercial Pt/C and RuO2/C catalysts. More importantly, the catalyst shows an unordinary stability, that is, is no obvious decrease after 30K cycles accelerated durability test for ORR and OER processes. The small potential gap (0.75 V) represents superior bifunctional ORR and OER activities of the FeCo/Se-CNT catalyst. The FeCo/Se-CNT catalyst possesses outstanding electrochemical performance for the rechargeable liquid and flexible all-solid-state Zn-air batteries, for example, a high open circuit voltage (OCV) and peak power density of 1.543 and 1.405 V and 173.4 and 37.5 mW cm-2, respectively.

16.
Chem Commun (Camb) ; 57(8): 1034-1037, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33409518

RESUMO

We report a highly concentrated electrolyte consisting of 4 M potassium bis(fluorosulfonyl)imide (KFSI) in diethylene glycol dimethyl ether (DEGDME). This new electrolyte enables stable cycling of K metal anodes with a high CE (over 98% over 400 cycles), and excellent capacity retention (99.7% after 500 cycles) of K||potassium Prussian blue (KPB) batteries.

17.
Chem Commun (Camb) ; 57(10): 1258-1261, 2021 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-33427245

RESUMO

In light of the surging research interest in disposable electronics, great demands have been imposed on compact power sources. Herein, a paper-based zinc-air battery that takes advantage of a hollow channel structure is reported. Unlike conventional paper-based metal-air batteries and fuel cells that tightly immobilize the electrode on the paper channel, a hollow channel layer containing potassium hydroxide solution electrolyte is sandwiched between the electrodes and paper channel layer. This novel zinc-air battery is capable of delivering a peak power density of 102 mW cm-2, surpassing state-of-the-art paper-based power sources. The superior power density originates from the boosted electrochemically active surface area of the cathode, which enhances the oxygen reduction reaction kinetics.

18.
J Phys Chem B ; 125(2): 528-538, 2021 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-33397114

RESUMO

The rigid-body fitting of predicted structural models into cryo-electron microscopy (cryo-EM) density maps is a necessary procedure for density map-guided protein structure determination and prediction. We proposed a novel multiobjective optimization protocol, MOFIT, which performs a rigid-body density-map fitting based on particle swarm optimization (PSO). MOFIT was tested on a large set of 292 nonhomologous single-domain proteins. Starting from structural models predicted by I-TASSER, MOFIT achieved an average coordinate root-mean-square deviation of 2.46 Å, which was 1.57, 2.79, and 3.95 Å lower than three leading single-objective function-based methods, where the differences were statistically significant with p-values of 1.65 × 10-6, 6.36 × 10-8, and 6.44 × 10-11 calculated using two-tail Student's t tests. Detailed analyses showed that the major advantages of MOFIT lie in the multiobjective protocol and the extensive PSO search simulations guided by the composite objective functions, which integrates complementary correlation coefficients from the global structure, local fragments, and individual residues with the cryo-EM density maps.

19.
Environ Monit Assess ; 192(12): 804, 2020 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-33263891

RESUMO

We investigated the occurrence and distribution of 18 antibiotics in surface water from Dongting Lake, as well as in influents and effluents from a municipal wastewater treatment plant (WWTP) and a swine farm. The total concentrations of target antibiotics in surface water ranged from limit of quantification to 3107 and 5.32-107 ng L-1 in the dry season and wet season, respectively. Among these studied antibiotics, ciprofloxacin (CIP) and lomefloxacin were as the main components in the dry season, while CIP, oxytetracycline, and chlortetracycline were the main components in the wet season. The concentrations of target compounds exhibited obvious temporal-spatial variation characteristic in the studied region, suggesting their different emission sources related to pig breeding, aquaculture, and human activities, as well as possible degradation in the aquatic system. We estimated that the daily total input and output masses of antibiotics in the influent and effluent from the swine farm were 12.1 mg d-1 pig-1 and 7.49 µg d-1 pig-1, while they were 103 µg d-1 inhabitant-1 and 22.9 µg d-1 inhabitant-1 in the WWTP. The risk assessment results indicated that CIP posed a moderate or high risk to algae in most locations in Dongting Lake.

20.
J Agric Food Chem ; 68(52): 15501-15508, 2020 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-33326242

RESUMO

Heterocyclic aromatic amines (HAAs) are potential human mutagens and carcinogens mainly generated in heat-treated meat. In this work, a broad-spectrum HAAs antibody was prepared and used to develop an indirect competitive ELISA (ic-ELISA) for simultaneous determination of eight HAAs, including 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f] quinoline (MeIQ), 2-amino-3-methylimidazo[4,5-f]quinoxaline (IQx), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx), 2-amino-3,7,8-trimethylimidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 2-amino-3,4,7,8-tetramethylimidazo[4,5-f]quinoxaline (4,7,8-TriMeIQx), and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in grilled and fried meat samples. The limit of detection (LOD, calculated as IC10) and 50% inhibition concentration (IC50) of ic-ELISA were 5.29 µg/L and 99.08 µg/L, respectively. The detection results of this ic-ELISA were in good agreement with the detection results of UPLC-MS/MS in real samples, which indicated that this ic-ELISA can be applied to detect the total content of eight HAAs in heat processed meat. Use of a broad-spectrum antibody is an efficient strategy in developing immunoassay for simultaneous measuring food risk factors with similar structure.


Assuntos
Aminas/química , Carcinógenos/química , Ensaio de Imunoadsorção Enzimática/métodos , Análise de Alimentos/métodos , Compostos Heterocíclicos/química , Produtos da Carne/análise , Animais , Anticorpos/análise , Bovinos , Peixes , Contaminação de Alimentos/análise , Manipulação de Alimentos , Suínos
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