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1.
J Biomed Mater Res B Appl Biomater ; 111(1): 51-61, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35799479

RESUMO

Spinal cord injury (SCI) will lead to irreversible damage of sensory and motor function of central nervous system, which seriously affects patient's quality of life. A variety of nerve engineering materials carrying various stem cells and cell growth factors had used to promote the repair of SCI, but they could not mimic the actual matric niche at spinal cord to promote cell proliferation and differentiation. Thus, developing novel biomaterial providing better niche of spinal cord is a new strategy to treat the severe SCI. In this study, we constructed porcine spinal cord decellularized matrix scaffold (SC-DM) with biocompatibility to load engineered basic fibroblast growth factor-overexpressing human umbilical cord mesenchymal stromal cells (bFGF-HUCMSCs) for treating SCI. The continuously released bioactive bFGF factors from grafted bFGF-HUCMSCs and three-dimensional niche by SC-DM promoted the differentiation of endogenous stem cells into neurons with nerve conduction function, leading a markedly motor function recovery of SCI. These results indicated that the functional bFGF-HUCMSCs/SC-DM scaffold provided more suitable matric niche for nerve cells, that would be a promising strategy for the clinical application of SCI.


Assuntos
Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Traumatismos da Medula Espinal , Humanos , Fator 2 de Crescimento de Fibroblastos/metabolismo , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/metabolismo , Qualidade de Vida , Traumatismos da Medula Espinal/metabolismo , Tecidos Suporte , Cordão Umbilical/citologia , Animais
2.
Food Chem ; 402: 134500, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36303390

RESUMO

A simple, rapid, and environmentally friendly microwave-assisted digestion method was developed and validated to determine heavy metals in aquatic products. The method could digest 640 samples using only one microwave digester in an 8 h working day. Only 2 mL of nitric acid was required to completely digest 0.2 g of samples, significantly reducing the usage of acid. The method was applied to investigate the heavy metals concentrations in mostly consumed aquatic products in Shenzhen. The investigation showed significant differences between different sampling times and sites in the concentrations of heavy metals, implying the necessity of sampling based on multiple times and sites for the accurate determination of heavy metals. Besides, iAs in crab were found to exceed the Chinese standard limit (GB 2762-2022). Health risk assessment showed that Cd and iAs in P. trituberculatus and R. philippinarum posed potential non-carcinogenic and carcinogenic risks to residents' health.


Assuntos
Metais Pesados , Micro-Ondas , Metais Pesados/análise , Medição de Risco , Ácido Nítrico , Digestão , Monitoramento Ambiental , China
3.
IEEE Trans Cybern ; PP2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36395126

RESUMO

Spatial-spectral classification (SSC) has become a trend for hyperspectral image (HSI) classification. However, most SSC methods mainly consider local information, so that some correlations may not be effectively discovered when they appear in regions that are not contiguous. Although many SSC methods can acquire spatial-contextual characteristics via spatial filtering, they lack the ability to consider correlations in non-Euclidean spaces. To address the aforementioned issues, we develop a new semisupervised HSI classification approach based on normalized spectral clustering with kernel-based learning (NSCKL), which can aggregate local-to-global correlations to achieve a distinguishable embedding to improve HSI classification performance. In this work, we propose a normalized spectral clustering (NSC) scheme that can learn new features under a manifold assumption. Specifically, we first design a kernel-based iterative filter (KIF) to establish vertices of the undirected graph, aiming to assign initial connections to the nodes associated with pixels. The NSC first gathers local correlations in the Euclidean space and then captures global correlations in the manifold. Even though homogeneous pixels are distributed in noncontiguous regions, our NSC can still aggregate correlations to generate new (clustered) features. Finally, the clustered features and a kernel-based extreme learning machine (KELM) are employed to achieve the semisupervised classification. The effectiveness of our NSCKL is evaluated by using several HSIs. When compared with other state-of-the-art (SOTA) classification approaches, our newly proposed NSCKL demonstrates very competitive performance. The codes will be available at https://github.com/yuanchaosu/TCYB-nsckl.

4.
J Adv Res ; 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36414168

RESUMO

INTRODUCTION: Drought is the principal abiotic stress that severely impacts cotton (Gossypium hirsutum) growth and productivity. Upon sensing drought, plants activate stress-related signal transduction pathways, including ABA signal and mitogen-activated protein kinase (MAPK) cascade. However, as the key components with fewest members in the MAPK cascade, the function and regulation of GhMKKs need to be elucidated. In addition, the relationship between MAPK module and the ABA core signaling pathway remain incompletely understood. OBJECTIVE: Here we aim to elucidate the molecular mechanism of cotton response to drought, with a focus on mitogen-activated protein kinase (MAPK) cascades activating ABA signaling. METHODS: Biochemical, molecular and genetic analysis were used to study the GhMAP3K62-GhMKK16-GhMPK32-EDT1 pathway genes. RESULTS: A nucleus- and membrane-localized MAPK cascade pathway GhMAP3K62-GhMKK16-GhMPK32, which targets and phosphorylates the nuclear-localized transcription factor GhEDT1, to activate downstream GhNCED3 to mediate ABA-induced stomatal closure and drought response was characterized in cotton. Overexpression of GhMKK16 promotes ABA accumulation, and enhances drought tolerance via regulating stomatal closure under drought stress. Conversely, RNAi-mediated knockdown of GhMKK16 expression inhibits ABA accumulation, and reduces drought tolerance. Virus-induced gene silencing (VIGS)-mediated knockdown of either GhMAP3K62, GhMPK32 or GhEDT1 expression represses ABA accumulation and reduces drought tolerance through inhibiting stomatal closure. Expression knockdown of GhMPK32 or GhEDT1 in GhMKK16-overexpressing cotton reinstates ABA content and stomatal opening-dependent drought sensitivity to wild type levels. GhEDT1 could bind to the HD boxes in the promoter of GhNCED3 to activate its expression, resulting in ABA accumulation. We propose that the MAPK cascade GhMAP3K62-GhMKK16-GhMPK32 pathway functions on drought response through ABA-dependent stomatal movement in cotton.

5.
Food Funct ; 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36382616

RESUMO

With the acceleration of the pace of life, people may face all kinds of pressure, and anxiety has become a common mental issue that is seriously affecting human life. Safe and effective food-derived compounds may be used as anti-anxiety compounds. In this study, anti-anxiety compounds were collected and curated for database construction. Quantitative structure-activity relationship (QSAR) models were developed using a combination of various machine-learning approaches and chemical descriptors to predict natural compounds in food with anti-anxiety effects. High-throughput molecular docking was used to screen out compounds that could function as anti-anxiety molecules by inhibiting γ-aminobutyrate transaminase (GABA-T) enzyme, and 7 compounds were screened for in vitro activity verification. Pharmacokinetic analysis revealed three compounds (quercetin, lithocholic acid, and ferulic acid) that met Lipinski's Rule of Five and inhibited the GABA-T enzyme to alleviate anxiety in vitro. The established QSAR model combined with molecular docking and molecular dynamics was proved by the synthesis and discovery of novel food-derived anti-anxiety compounds.

6.
Artigo em Inglês | MEDLINE | ID: mdl-36378388

RESUMO

Previous findings have reported the role of different types of heavy metals in cardiometabolic diseases. In the present research, we aim to evaluate the association between blood cadmium levels and Metabolic Syndrome (MetS) based on the large-sample NHANES data. Public availably data from NHANES 2017-2020 cycle was obtained. Participants were divided into MetS and non-MetS groups according to waist circumference (WC), triglyceride (TG), high-density lipoprotein (HDL), blood pressure (BP) and fasting plasma glucose (FPG) levels based on the National Cholesterol Education Program (NCEP) criteria. Student's t test, Mann-Whitney U test, and Chi-square test were performed for univariate analysis. Multivariate logistic analysis was performed to explore the relationship between blood cadmium and MetS and research findings were presented in forest plot. We also investigated the association of blood cadmium and MetS in subgroups stratified by age, gender and race. Population with MetS had significantly higher levels of blood [0.30 (0.18-0.54) vs. 0.24 (0.15-0.46) ug/L, p < 0.001] and urinary cadmium levels [0.29 (0.17-0.52) vs. 0.20 (0.09-0.42) ug/L, p < 0.001] compared with those without MetS. Higher blood cadmium concentrations were also observed in participants with elevated WC (0.28 vs. 023 ug/L, p < 0.001], TG (0.28 vs. 0.26 ug/L, p = 0.029), BP (0.33 vs. 0.23 ug/L, p < 0.001) and FPG (0.29 vs. 0.24 ug/L, p < 0.001) compared with those with normal metabolic parameters. Multivariate logistic regression showed that one-unit increasement of blood cadmium was associated with 1.25 times higher prevalence ratios for MetS after adjusting potential confounders (95% CI: 1.06-1.48, p = 0.0083). The associations between serum cadmium concentrations and MetS components were then evaluated, and the results showed higher blood cadmium levels were associated with higher risk for elevated TG, low HDL and elevated BP when treated as continuous variable. When treated as categorical variable, only BP was found positively associated with blood cadmium. Stratified multiple logistic regression analysis indicated that the positive association between blood cadmium and MetS remained significant in subjects less than 60 years old and female subgroup. In conclusion, the cross-sectional survey suggested the positive association between blood cadmium levels and risk for MetS, prospective research need to be conducted for further evaluation of the causal relationship between blood cadmium and MetS.

7.
Phys Chem Chem Phys ; 24(44): 27406-27412, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36331370

RESUMO

Two modified V2C-MXene nanocoatings are prepared through different molecular weights of polyacrylic acid (polyacrylic acid with ∼4 50 000 is marked as LPAA, and polyacrylic acid with ∼4 000 000 is marked as HPAA) and two-dimensional V2C-MXene. Their properties are characterized using a ball-on-disc tribometer, three-dimensional white-light interferometry topography images, optical microscope, Raman spectrometer, focused ion beam/scanning electron microscope and high-resolution transmission electron microscope/energy dispersive X-ray spectrometer (HRTEM/EDS). As a result, an ultralow friction (µ ≈ 0.073 ± 0.024) and an ultralow wear (3.41 × 10-7 mm3 N-1 m-1 for ball scar, and 7.49 × 10-8 mm3 N-1 m-1 for disc track) are achieved for the LPAA@V2C vs. steel ball system tested under 4 N in the air through tribo-physicochemical interactions. During the rubbing process, the LPAA@V2C nanocoating is transferred onto counter-bodies to form mixed-phase lubricative tribofilms. Monitoring via a HRTEM/EDS, the mixed-phase lubricative tribofilms are found to be mainly composed of amorphous carbon phases containing O and V and layered nano-debris along the sliding surface. The tribofilm's stable structure is the key to realizing ultralow friction and ultralow wear through the LPAA modification. These findings disclose that MXene-based nanomaterials can be applied for material engineering and mechanical engineering under common working conditions.

8.
J Environ Manage ; 326(Pt A): 116654, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36368197

RESUMO

Increasing extreme temperatures are producing a serious impact on the economies of cities. However, the importance of social factors is typically neglected by the existing research. In this work, we first establish a supply-demand-public expenditure (SDP) framework for assessing and forecasting heat-related economic loss. Compared with the previous framework, SDP possesses a more comprehensive index system and functions that apply to all types of cities. We selected different economic development and geographical locations (Nanjing, Suzhou, and Yancheng) as case studies to verify the wide applicability of the SDP framework. A qualitative analysis and quantitative prediction of heatwaves and socioeconomic factors on losses were conducted for different cities. The results showed that different loss types displayed obvious regional heterogeneity among the cities. The labor value loss was the most significant type, and health loss was the most vulnerable type. In addition, public expenditure played a neglected critical regulatory role. Apart from these, the current level of public expenditure for heat prevention and control remains insufficient. Based on an assessment of the effects of interventions, policymakers need to make more efforts to increase the proportion of heat-related public spending and ensure stable socio-economic development by utilizing pathways with positive intervention potentials.

9.
Water Res ; 227: 119325, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36371917

RESUMO

Phosphorus (P) harvesting from source-separated urine to optimize the overall nutrient loop is one of the most appealing benefits and is a global research interest in wastewater management and treatment. However, current P precipitation is mainly oriented to struvite, which is limited by the issues such as relatively low product purity and high cost of Mg source. Distinguished from previous conventional struvite precipitation, the strategy of precisely harvesting P from fresh human urine as high-purity calcium phosphate was first proposed in this study. This enhanced strategy can optimize P harvesting performance and product purity by simply regulating the consumption of calcium-based materials via model simulation and experimental validation. The thermodynamic model was constructed to probe the precipitation conversion mechanism, and visually predict the component and yield for products under various operating conditions. Batch experiments were conducted to investigate P recovery performance as a function of initial Mg2+ concentration, initial pH level, as well as degree of urine hydrolysis. Moreover, the alternative dosing scheme with different calcium salts and alkali was presented, diversifying the options for efficient P recovery. The results showed that, from the perspective of acidic storage for fresh urine, P recovery can be boosted along with eliminating urine hydrolysis. In urine with an initial pH=2.0, P can be completely recovered and purity for calcium phosphate can be optimized to 100% within a Ca/P ratio range of 1.67-2.3. Overall, this work is of great significance for precisely and efficiently harvesting P from urine and provides an integrated strategy for P resource recovery from urine.


Assuntos
Fosfatos , Fósforo , Humanos , Fósforo/urina , Estruvita , Cálcio , Compostos de Magnésio , Fosfatos de Cálcio , Precipitação Química
10.
Water Res ; 227: 119323, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36395565

RESUMO

Nowadays metal-free persulfate-based advanced oxidation processes (AOPs) have been intensively investigated, however, the catalysts are often too complex to fully consider their application potential. Conventional AOPs usually suffer from severe interference in real water matrix, thus, selective oxidation is practically and scientifically challenging as it could avoid unnecessary inputs of energy and possible secondary pollutants. In this study, a remarkably synergistic effect was achieved when conventional amorphous boron/peroxymonosulfate (Boron/PMS, 0.67 × 10-2 min-1) system was combined with electrolysis (E-Boron/PMS, 1.54 × 10-2 min-1) to degrade sulfamethoxazole (SMX). Evidenced by selectively quenching tests with kinetic evaluation, electron paramagnetic resonance (EPR), solvent-exchange experiment and electrochemical analysis, the dominated reactive oxygen species in E-Boron/PMS system tended to be 1O2, instead of the •OH and SO4•-. Mechanistic study unveiled that 1O2 was generated via accelerated PMS self-decomposition, triggered by interface alkalization and hydroxyl radicals transfer at the cathode interface. 1O2 is considered to be selective to the electron-rich organic compounds, thus E-Boron/PMS system was superior to conventional radical-dominated system (Boron/PMS) for SMX removal in the co-presence of common inorganic anions, showing the great merits of selective oxidation in nonradical system. These findings provided new insights into effective and selective oxidation of SMX via E-Boron/PMS system, which shed new light on the development of nonradical system.


Assuntos
Boro , Peróxidos , Peróxidos/química , Metais , Oxirredução , Sulfametoxazol
11.
J Med Virol ; 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36418175

RESUMO

With a large population most susceptible to Omicron and emerging SARS-CoV-2 variants, China faces uncertain scenarios if reopening its border. Thus, we aimed to predict the impact of combination preventative interventions on hospitalization and death. An age-stratified susceptible-infectious-quarantined-hospitalized-removed-susceptible (SIQHRS) model based on the new guidelines of COVID-19 diagnosis and treatment (the ninth edition) was constructed to simulate the transmission dynamics of Omicron within 365 days. At baseline, we assumed no interventions other than 60% booster vaccination in individuals aged <=60 years and 80% in individuals aged >60 years, quarantine and hospitalization. Oral antiviral medications for COVID-19 (e.g. BRII-196/BRII-198) and non-pharmaceutical interventions (NPIs) such as social distancing and antigen self-testing were considered in subsequent scenarios. Sensitivity analyses were conducted to reflect different levels of interventions. A total of 0.73 billion cumulative quarantines (95% CI 0.53-0.83), 33.59 million hospitalizations (22.41-39.31), and 0.62 million deaths (0.40-0.75) are expected in 365 days. The case fatality rate with pneumonia symptoms (moderate, severe and critical illness) is expected to be 1.83% (1.68-1.99%) and the infected fatality rate 0.38‰ (0.33-0.42‰). The highest existing hospitalization and ICU occupations are 3.11 (0.30-3.85) and 20.33 (2.01-25.20) times of capacity, respectively. Sensitivity analysis showed that interventions can be adjusted to meet certain conditions to reduce the total number of infections and deaths. In conclusion, after sufficient respiratory and ICU beds are prepared and the relaxed NPIs are in place, the SARS-CoV-2 Omicron variant would not seriously impact the health system. This article is protected by copyright. All rights reserved.

12.
Eur Radiol ; 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36322193

RESUMO

OBJECTIVES: This prospective study compared the detection efficacy of analog 18F-fluoro-2-deoxyglucose (18F-FDG) positron emission tomography (PET)/computed tomography (CT) (aF PET/CT), digital [18F]FDG PET/CT (dF PET/CT), and digital 13N-ammonia (13N-NH3) PET/CT (dN PET/CT) for patients with lung adenocarcinoma featuring ground glass nodules (GGNs). METHODS: Eighty-seven patients with lung adenocarcinoma featuring GGNs who underwent dF and dN PET/CT were enrolled. Based on the GGN component, diameter, and solid-part size, 87 corresponding patients examined using aF PET/CT were included, with age, sex, and lesion characteristics closely matched. Images were visually evaluated, and the tumor to background ratio (TBR) was used for semi-quantitative analysis. RESULTS: Ultimately, 40 and 47 patients with pure GGNs (pGGNs) and mixed GGNs (mGGNs), respectively, were included. dF PET/CT revealed more positive lesions and higher tracer uptake in GGNs than did aF PET/CT (53/87 vs. 26/87, p < 0.05; TBR: 3.08 ± 4.85 vs. 1.42 ± 0.93, p < 0.05), especially in mGGNs (44/47 vs. 26/47, p < 0.05; TBR: 4.48 ± 6.17 vs. 1.78 ± 1.16, p < 0.05). However, dN PET/CT detected more positive lesions than did dF PET/CT (71/87 vs. 53/87, p < 0.05), especially in pGGNs (24/40 vs. 9/40, p < 0.05). CONCLUSIONS: dF PET/CT provides superior detection efficacy over aF PET/CT for patients with lung adenocarcinoma featuring GGNs, particularly mGGNs. dN PET/CT revealed superior detection efficacy over dF PET/CT, particularly in pGGNs. aF, dF, and dN PET/CT are valuable non-invasive examinations for lung cancer featuring GGNs, with dN PET/CT offering the best detection performance. KEY POINTS: • Digital PET/CT provides superior detection efficacy over analog PET/CT in patients with lung adenocarcinoma featuring GGNs. • dN PET/CT can offer more help in the early detection of malignant GGN.

13.
Heart Surg Forum ; 25(5): E660-E664, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36317906

RESUMO

BACKGROUND: Acute kidney injury (AKI) is one of the most frequent complications after coronary artery bypass grafting. Previous studies have shown that diabetes is a key pathogenic factor. But how diabetes is related to AKI in off-pump CABG patients still is in debate. Here, we aim to study the relationship between diabetes and AKI after off-pump coronary artery bypass grafting (off-pump CABG). METHODS: Patients who underwent off-pump CABG from April 2017 to December 2020 in The First Affiliated Hospital of USTC were enrolled in this retrospective study. AKI was defined and classified, according to the criteria proposed by the Acute Kidney Injury Network. The incidence risk of acute kidney injury was measured by logistic regression and compared. RESULTS: A total of 395 patients, who underwent off-pump CABG, were included in this study. The postoperative acute kidney injury rate for a patient with diabetes was significantly higher than for patients without diabetes (x2 = 5.09, P = 0.024). Logistic regression analysis showed that patients with diabetes have a much higher risk with acute kidney injury occurring after off-pump coronary artery bypass grafting (OR 1.852, 95% CI 1.161 - 2.954, P = 0.01). CONCLUSIONS: Diabetes is an independent risk factor for postoperative AKI for patients undergoing off-pump CABG.


Assuntos
Injúria Renal Aguda , Ponte de Artéria Coronária sem Circulação Extracorpórea , Diabetes Mellitus , Humanos , Estudos Retrospectivos , Ponte de Artéria Coronária sem Circulação Extracorpórea/efeitos adversos , Injúria Renal Aguda/etiologia , Ponte de Artéria Coronária/efeitos adversos
14.
Nat Prod Res ; : 1-6, 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36323318

RESUMO

Colletotrin A (1), a new 1,4-benzoxazine derivative, and eight known compounds, including two alkaloids (2-3), one acylamide (4), one benzaldehyde (5), and four sterols (6-9) were obtained from endophytic fungus Colletotrichum gloeosporioides B-142, which was isolated from Dracaena cochinchinensis. Among them, 1,2,3,4-tetrahydroquinoline-4,8-diol (2) was reported for the first time as natural product. The structure of compound 1 was elucidated with help of spectroscopic data including IR, UV, electronic circular dichroism (ECD) calculation, HRESIMS, 1D and 2D NMR (COSY, HSQC and HMBC). The antimicrobial activities were evaluated by disc diffusion method.

15.
Transl Neurodegener ; 11(1): 49, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36376937

RESUMO

BACKGROUND: Microbiome-gut-brain axis may be involved in the progression of age-related cognitive impairment and relevant brain structure changes, but evidence from large human cohorts is lacking. This study was aimed to investigate the associations of gut microbiome with cognitive impairment and brain structure based on multi-omics from three independent populations. METHODS: We included 1430 participants from the Guangzhou Nutrition and Health Study (GNHS) with both gut microbiome and cognitive assessment data available as a discovery cohort, of whom 272 individuals provided fecal samples twice before cognitive assessment. We selected 208 individuals with baseline microbiome data for brain magnetic resonance imaging during the follow-up visit. Fecal 16S rRNA and shotgun metagenomic sequencing, targeted serum metabolomics, and cytokine measurements were performed in the GNHS. The validation analyses were conducted in an Alzheimer's disease case-control study (replication study 1, n = 90) and another community-based cohort (replication study 2, n = 1300) with cross-sectional dataset. RESULTS: We found protective associations of specific gut microbial genera (Odoribacter, Butyricimonas, and Bacteroides) with cognitive impairment in both the discovery cohort and the replication study 1. Result of Bacteroides was further validated in the replication study 2. Odoribacter was positively associated with hippocampal volume (ß, 0.16; 95% CI 0.06-0.26, P = 0.002), which might be mediated by acetic acids. Increased intra-individual alterations in gut microbial composition were found in participants with cognitive impairment. We also identified several serum metabolites and inflammation-associated metagenomic species and pathways linked to impaired cognition. CONCLUSIONS: Our findings reveal that specific gut microbial features are closely associated with cognitive impairment and decreased hippocampal volume, which may play an important role in dementia development.


Assuntos
Microbioma Gastrointestinal , Humanos , Microbioma Gastrointestinal/genética , RNA Ribossômico 16S/genética , Estudos Transversais , Estudos de Casos e Controles , Cognição , Encéfalo/diagnóstico por imagem
16.
Front Public Health ; 10: 1010539, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388381

RESUMO

Objective: To explore the association between egg intake and cardiometabolic factors (CMFs) in Chinese adults. Method: The subjects were 6,182 adults aged 18-64 who had complete survey data and had no CMFs at baseline. Egg intake was assessed with 3 days-24 h dietary recalls in all waves of the China Health and Nutrition Survey (CHNS). Multivariate Cox proportional risk regression model and restricted cubic spline (RCS) model were used to analyze the association and dose-response relationship between egg intake and CMFs. Results: Of the 6,182 participants who did not have metabolic syndrome (MetS) at baseline, 1,921 developed this disease during an average follow-up of 5.71 years, with an incidence of 31.07%. Central obesity, elevated TG, decreased HDL-C, elevated blood pressure and elevated plasma glucose were 38.65, 26.74, 30.21, 40.64, and 30.64%, respectively. After adjusting for demographic characteristics, lifestyle, energy and BMI, using the lowest quintile (Q1) as a reference, the risk of central obesity, elevated TG, decreased HDL-C, and elevated plasma glucose in the highest quintile (Q5) were reduced by 15% (HR = 0.85, 95% CI = 0.73-0.98, P = 0.16), 33% (HR = 0.67, 95% CI = 0.57-0.78), 25% (HR = 0.75, 95% CI = 0.63 0.90, p = 0.05), and 28% (HR = 0.72, 95% CI = 0.63-0.83, p < 0.05), respectively. The risk of elevated blood pressure was reduced by 26% in the fourth quintile (HR = 0.74, 95% CI = 0.64-0.85, P = 0.85). RCS analysis show that the overall correlation and nonlinear relationship between egg intake and CMFs were statistically significant (P < 0.05). When the intake was lower than 20 g/days, the risk of MetS, central obesity, elevated blood pressure and elevated plasma glucose were negatively correlated with egg intake, while elevated TG was negatively correlated with eggs when the intake was lower than 60 g/days. There was no statistically significant association between egg intake and CMFs at higher egg intake. Conclusion: There was a U-shaped association between egg intake and CMFs in Chinese adults.


Assuntos
Hipertensão , Síndrome Metabólica , Adulto , Humanos , Obesidade Abdominal/epidemiologia , Obesidade Abdominal/complicações , Glicemia , Fatores de Risco , China/epidemiologia , Síndrome Metabólica/epidemiologia , Síndrome Metabólica/etiologia , Obesidade
17.
Biomolecules ; 12(11)2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36421715

RESUMO

Rickettsiales (Rickettsia spp., Ehrlichia spp., and Anaplasma spp., etc.) are generally recognized as potentially emerging tick-borne pathogens. However, some bacteria and areas in China remain uninvestigated. In this study, we collected 113 ticks from mammals in Guizhou Province, Southwest China, and screened for the Rickettsiales bacteria. Subsequently, two spotted fever group Rickettsia species and one Candidatus Lariskella sp. were detected and characterized. "Candidatus Rickettsia jingxinensis" was detected in Rhipicephalus microplus (1/1), Haemaphysalis flava (1/3, 33.33%), Haemaphysalis kitaokai (1/3), and Ixodes sinensis (4/101, 3.96%), whereas Rickettsia monacensis was positive in H. flava (1/3), H. kitaokai (2/3), and I. sinensis ticks (74/101, 73.27%). At least two variants/sub-genotypes were identified in the R. monacensis isolates, and the strikingly high prevalence of R. monacensis may suggest a risk of human infection. Unexpectedly, a Candidatus Lariskella sp. belonging to the family Candidatus Midichloriaceae was detected from Ixodes ovatus (1/4) and I. sinensis (10/101, 9.90%). The gltA and groEL gene sequences were successfully obtained, and they show the highest (74.63-74.89% and 73.31%) similarities to "Candidatus Midichloria mitochondrii", respectively. Herein, we name the species "Candidatus Lariskella guizhouensis". These may be the first recovered gltA and groEL sequences of the genus Candidatus Lariskella.

18.
Brain Sci ; 12(11)2022 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-36358391

RESUMO

The heat-sink effect and thermal damage of conventional thermal ablative technologies can be minimized by irreversible electroporation (IRE), which results in clear ablative boundaries and conservation of blood vessels, facilitating maximal safe surgical resection for glioblastoma. Although much comparative data about the death forms in IRE have been published, the comprehensive genetic regulatory mechanism for apoptosis, among other forms of regulatory cell death (RCD), remains elusive. We investigated the electric field intensity threshold for apoptosis/necrosis (YO-PRO-1/PI co-staining) of the U251 human malignant glioma cell line with stepwise increased uniform field intensity. Time course samples (0-6 h) of apoptosis induction and sham treatment were collected for transcriptome sequencing. Sequencing showed that transcription factor AP-1 and its target gene Bim (Bcl2l11), related to the signaling pathway, played a major role in the apoptosis of glioma after IRE. The sequencing results were confirmed by qPCR and Western blot. We also found that the transcription changes also implicated three other forms of RCD: autophagy, necroptosis, and immunogenic cell death (ICD), in addition to apoptosis. These together imply that IRE possibly mediates apoptosis by the AP-1-Bim pathway, causes mixed RCD simultaneously, and has the potential to aid in the generation of a systemic antitumor immune response.

19.
Artigo em Inglês | MEDLINE | ID: mdl-36434445

RESUMO

Exogenous and endogenous exposure to aldehydes is seen worldwide. Aldehydes are closely associated with human diseases, especially reproductive toxicity. However, the effect of aldehyde exposure on sex steroid hormones among adults remains uninvestigated. A total of 851 participants aged over 18 years were included in this cross-sectional analysis based on data from National Health and Nutrition Examination Survey (NHANES) 2013-2014. Serum aldehyde concentrations were quantified following an automated analytical method. Sex steroid hormones including total testosterone, estradiol, and sex hormone binding globulin (SHBG) were detected. Multivariate linear regression models, forest plots, generalized additive model (GAM), and smooth curve fitting analysis were used to assess the associations between quartiles of aldehydes and sex steroid hormones levels after adjusting for potential confounders. Butyraldehyde and propanaldehyde were found to be negatively associated with estradiol and SHBG in females and males, respectively. ß values with 95% confidence intervals (95% CIs) were - 20.59 (- 38.30 to - 2.88) for Q2 vs. Q1 of butyraldehyde and - 8.13 (- 14.92 to - 1.33) and - 7.79 (- 14.91 to - 0.67) for Q2 vs. Q1 and Q4 vs. Q1 of propanaldehyde. No significant associations were observed between other aldehydes and sex hormones. In premenopausal women, isopentanaldehyde was inversely associated with serum total testosterone levels (Q4 vs. Q1: OR = - 7.95, 95% CI: - 15.62 to - 0.27), whereas propanaldehyde was positively associated with serum estradiol concentration (Q3 vs. Q1: ß = 28.88, 95% CI: 0.83 to 56.94). Compared with Q1, Q3 of isopentanaldehyde was associated with 3.53 pg/mL higher concentration of estradiol in postmenopausal women (ß = 3.53, 95% CI: 0.08 to 6.97). Moreover, in males under 40 years, butyraldehyde and heptanaldehyde were inversely proportional to total testosterone levels and heptanaldehyde and butyraldehyde were negatively associated with estradiol and SHBG. Decreased total testosterone, elevated estradiol, and decreased SHBG levels were found in higher quartiles of benzaldehyde, hexanaldehyde and isopentanaldehyde, and propanaldehyde, respectively, in males aged over 60 years. In male participants aged 40-60 years, only hexanaldehyde was observed to be correlated with higher serum estradiol levels. In conclusion, our current research presented the association between six serum aldehydes and sex hormones. Of note, stratification analyses were conducted in participants with different menopausal statuses and age among males and females. Sex- and age-specific effect of aldehyde exposure on alterations in sex hormone levels were observed. Further studies are warranted to confirm the causal relationship and explore the underlying mechanisms.

20.
Front Public Health ; 10: 974848, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36339206

RESUMO

Background: The coronavirus disease (COVID-19) pandemic, which has been ongoing for more than 2 years, has become one of the largest public health issues. Vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is one of the most important interventions to mitigate the COVID-19 pandemic. Our objective is to investigate the relationship between vaccination status and time to seroconversion. Methods: We conducted a cross-sectional observational study during the SARS-CoV-2 B.1.617.2 outbreak in Jiangsu, China. Participants who infected with the B.1.617.2 variant were enrolled. Cognitive performance, quality of life, emotional state, chest computed tomography (CT) score and seroconversion time were evaluated for each participant. Statistical analyses were performed using one-way ANOVA, univariate and multivariate regression analyses, Pearson correlation, and mediation analysis. Results: A total of 91 patients were included in the analysis, of whom 37.3, 25.3, and 37.3% were unvaccinated, partially vaccinated, and fully vaccinated, respectively. Quality of life was impaired in 30.7% of patients, especially for mental component summary (MCS) score. Vaccination status, subjective cognitive decline, and depression were risk factors for quality-of-life impairment. The chest CT score mediated the relationship of vaccination status with the MCS score, and the MCS score mediated the relationship of the chest CT score with time to seroconversion. Conclusion: Full immunization course with an inactivated vaccine effectively lowered the chest CT score and improved quality of life in hospitalized patients. Vaccination status could influence time to seroconversion by affecting CT score and MCS score indirectly. Our study emphasizes the importance of continuous efforts in encouraging a full vaccination course.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , Pandemias , Vacinas contra COVID-19 , Soroconversão , COVID-19/prevenção & controle , Saúde Mental , Estudos Transversais , Qualidade de Vida , Tomografia Computadorizada por Raios X , Vacinação
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