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1.
J Nanosci Nanotechnol ; 20(2): 779-788, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383073

RESUMO

N and F co-doped La-TiO2 (La-TONF) samples were prepared through the solvothermal method by using HMT and NaF as precursors. The obtained samples were characterized by UV-Vis DRS, XRD, XPS and PL measurements for light-harvesting properties, crystal phase and optical characteristics, respectively. Interestingly, the TONF sample had a different fluorescence emission intensity than the TON or TOF samples, thus suggesting a clear synergistic effect of N and F co-doping. The optimal doping amount of La was 2 wt.%, and the absorption edge was red-shifted from 453 nm to 464 nm for La-TiO2 and La-TONF. The photocatalytic activity was evaluated by degradation of MO and oxidation of TMB under visible light irradiation. La-TONF exhibited excellent photocatalytic activity and a degradation rate of 92.4%, 4.4 times that of undoped TiO2 (20.8%). The photocatalytic degradation activity remained above 85.8%, even after five runs. In addition, the MO photodegradation catalyzed by La-TONF followed first order kinetics. According these results, a possible synergistic effect mechanism for the enhanced photocatalytic performance is proposed.

2.
Int J Mol Med ; 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31485644

RESUMO

Asthma is a common chronic airway inflammatory disease, characterized by airway inflammation and remodeling. Piperlongumine (PL) has a number of physiological and pharmacological properties. However, the anti­asthmatic effect of PL has not been reported to date. In the present study, ovalbumin (OVA) was used to sensitize and challenge mice to induce asthma. The results revealed that PL pretreatment reduced OVA­induced airway inflammatory cell infiltration, reduced Th2 cytokine expression, both in the bronchoalveolar lavage fluid and in lung tissues, reduced the serum IgE level, pro­inflammatory cytokine [tumor necrosis factor (TNF)­α and interleukin (IL)­6] and intercellular adhesion molecule expression, as well as nuclear factor (NF)­κB activation. In addition, PL also mitigated OVA­induced goblet cell metaplasia, inhibited mucus protein secretion, mitigated airway fibrosis and downregulated fibrosis marker expression. It was also demonstrated that PL inhibited TNF­α induced inflammatory cytokine expression and NF­κB activation in vitro. Taken together, the findings of the present study indicated that PL can reduce OVA­induced airway inflammation and remodeling in asthmatic mice, and that these effects may be mediated by inhibiting NF­κB signaling.

3.
Ophthalmology ; 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31471087

RESUMO

PURPOSE: To describe the distribution of corneal hysteresis (CH) in a large cohort and explore its associated factors and possible clinical applications. DESIGN: Cross-sectional study within the UK Biobank, a large cohort study in the United Kingdom. PARTICIPANTS: We analyzed CH data from 93 345 eligible participants in the UK Biobank cohort, aged 40 to 69 years. METHODS: All analyses were performed using left eye data. Linear regression models were used to evaluate associations between CH and demographic, lifestyle, ocular, and systemic variables. Piecewise logistic regression models were used to explore the relationship between self-reported glaucoma and CH. MAIN OUTCOME MEASURES: Corneal hysteresis (mmHg). RESULTS: The mean CH was 10.6 mmHg (10.4 mmHg in male and 10.8 mmHg in female participants). After adjusting for covariables, CH was significantly negatively associated with male sex, age, black ethnicity, self-reported glaucoma, diastolic blood pressure, and height. Corneal hysteresis was significantly positively associated with smoking, hyperopia, diabetes, systemic lupus erythematosus (SLE), greater deprivation (Townsend index), and Goldmann-correlated intraocular pressure (IOPg). Self-reported glaucoma and CH were significantly associated when CH was less than 10.1 mmHg (odds ratio, 0.86; 95% confidence interval, 0.79-0.94 per mmHg CH increase) after adjusting for covariables. When CH exceeded 10.1 mmHg, there was no significant association between CH and self-reported glaucoma. CONCLUSIONS: In our analyses, CH was significantly associated with factors including age, sex, and ethnicity, which should be taken into account when interpreting CH values. In our cohort, lower CH was significantly associated with a higher prevalence of self-reported glaucoma when CH was less than 10.1 mmHg. Corneal hysteresis may serve as a biomarker aiding glaucoma case detection.

4.
Med Sci Monit ; 25: 6598-6604, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31477682

RESUMO

BACKGROUND The aim of this study was to evaluate the influence of distal radius fractures (DRFs) malalignment on the treatment outcomes in patients over age 65 years. MATERIAL AND METHODS We retrospectively reviewed the records on fresh DRFs treated with closed reduction from December 2014 to January 2018. After treatment, patients were evaluated for the determination of grip strength, the Visual Analog Scale (VAS) during wrist movement, the Patient-Rated Wrist Evaluation (PRWE), the Disabilities of the Arm, Shoulder and Hand (DASH) score, the appearance satisfaction, and active wrist range of motion (ROM). RESULTS A total of 96 patients with complete data were included in our study. During follow-up, there were 75 patients (78.1%) with acceptable reduction and 21 patients (21.9%) with unacceptable reduction. Compared with those having acceptable alignment in the distal radius, patients with unacceptable alignment had weak grip strength, were unsatisfied appearance, and had severe flexion as well as ulnar deviation limitation at 6-month follow-up. A significant correlation was found between ulnar positive variance and grip strength (r=-0.35, P=0.03), as well as dorsal angulation and flexion movement (r=-0.31, P=0.02). CONCLUSIONS Conservative treatment should be used differently, even in elderly patients. For low-demand patients, it is not necessary to restore all anatomic radiographic parameters, as malalignment does not increase disability or pain score. However, for patients who are still healthy and active, satisfactory reduction is the first choice, as malalignment can lead to decreased grip strength, dissatisfaction with appearance, and certain wrist limitations.

5.
Org Biomol Chem ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31478050

RESUMO

A method for metal-free, 1,3-dibromo-5,5-dimethylhydantoin mediated methyl C(sp3)-H bond azolation of thioanisoles has been developed, affording a facile route for the construction of nitrogen-functionalized thioanisoles, possibly via a nitrogen-centered radical process. This reaction represents an important addition to the limited number of existing methods for the methyl C(sp3)-H bond functionalization of thioanisoles, and may find practical application in the synthesis of nitrogen-alkylated azoles.

6.
Biochimie ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31491441

RESUMO

ArgR, a transcriptional regulator belonging to the AraC/XylS family, plays a key role in arginine metabolism regulation. ArgR has also been found to repress the transcription of a lipase gene, but its molecular mechanism is still unknown. In this study, we investigated the molecular mechanism acting on the expression of intracellular lipase gene lipA regulated by ArgR in Pseudomonas protegens Pf-5 through knockout and overexpression of argR, detection of DNA-protein interaction in vivo, determining whole-cell lipase activities of various strains derived from Pf-5, and examining ß-galactosidase activities of various lacZ fusions. The results demonstrated that ArgR inhibits lipA expression at the transcriptional level. Further results showed that the inhibition of lipA transcription by ArgR is mediated by binding to the ArgR binding site of lipA promoter to produce steric hindrance, in which the common sequence, TGTCGC is crucial for the ArgR binding. Besides, arginine inhibits lipA expression in both wild-type and argR mutant, and shows a synergistic inhibition on lipA expression when combined with ArgR. To the best of our knowledge, this is the first report on ArgR directly repressing the transcription of lipase gene.

7.
J Chem Phys ; 151(9): 094302, 2019 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-31492073

RESUMO

The excited-state intramolecular proton transfer (ESIPT) process and subsequent electronic relaxation dynamics in methyl salicylate have been investigated using femtosecond time-resolved ion yield spectroscopy combined with time-resolved photoelectron imaging. Excitation with a tunable pump pulse populates the keto tautomer in the first excited electronic state S1(ππ*). As a hydrogen atom transfers from the phenolic group to the carbonyl group within 100 fs, the molecular geometry changes gradually, leading to a variation in the electronic photoionization channel. By virtue of the accidental resonance with some intermediate Rydberg states, the time-dependent photoelectron spectra provide a direct mapping of the ESIPT reaction from the initially populated keto tautomer to the proton-transferred enol tautomer. Subsequently, the population around the enol configuration undergoes intramolecular vibrational redistribution on a subpicosecond time scale, followed by internal conversion to the ground state with a wavelength-dependent lifetime in the picosecond range. Furthermore, the excitation energies of several Rydberg states in methyl salicylate are determined experimentally.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31494266

RESUMO

To unravel the genetic mechanisms of disease and physiological traits, it requires comprehensive sequencing analysis of large sample size in Chinese populations. Here, we report the primary results of the Chinese Academy of Sciences Precision Medicine Initiative (CASPMI) project launched by the Chinese Academy of Sciences, including the de novo assembly of a northern Han reference genome (NH1.0) and whole genome analyses of 597 healthy people coming from most areas in China. Given the two existing reference genomes for Han Chinese (YH and HX1) were both from the south, we constructed NH1.0, a new reference genome from a northern individual, by combining the sequencing strategies of PacBio, 10X Genomics, and Bionano mapping. Using this integrated approach, we obtained an N50 scaffold size of 46.63 Mb for the NH1.0 genome and performed a comparative genome analysis of NH1.0 with YH and HX1. In order to generate a genomic variation map of Chinese populations, we performed the whole-genome sequencing of 597 participants and identified 24.85 million (M) single nucleotide variants (SNVs), 3.85 M small indels, and 106,382 structural variations. In the association analysis with collected phenotypes, we found that the T allele of rs1549293 in KAT8 significantly correlated with the waist circumference in northern Han males. Moreover, significant genetic diversity in MTHFR, TCN2, FADS1, and FADS2, which associate with circulating folate, vitamin B12, or lipid metabolism, was observed between northerners and southerners. Especially, for the homocysteine-increasing allele of rs1801133 (MTHFR 677T), we hypothesize that there exists a "comfort" zone for a high frequency of 677T between latitudes of 35-45 degree North. Taken together, our results provide a high-quality northern Han reference genome and novel population-specific data sets of genetic variants for use in the personalized and precision medicine.

9.
J Orthop Surg Res ; 14(1): 295, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484547

RESUMO

OBJECTIVE: To investigate the incidence of postoperative overall complications or secondary procedures following distal radius fractures treated by volar locking plate (VLP) METHODS: Electronic medical records (EMR) of 1152 patients with 1175 distal radius fractures treated by volar locking plate between January 2013 and September 2018 were retrospectively reviewed and the data were extracted. The picture archiving and communication system (PACS) was inquired to assess the fracture severity and to determine the fracture type. Univariate and multivariate logistic regression analyses were used to identify the associated risk factors. RESULTS: During the median follow-up period of 6 months, a total of 138 complications in 131 patients were determined, indicating the accumulated rate of 11.7%; there were 68 cases of secondary procedures, with the rate of 5.8%. The independent associated factors for postoperative overall complications were AO type C fracture (OR, 2.6; 95%CI, 1.2 to 4.0), open fracture (OR, 4.2; 95%CI, 1.9 to 6.5), and significant collapse of the lunate fossa (OR, 2.9; 95%CI, 13 to 4.3), and for secondary procedures were significant collapse of the lunate fossa (OR, 3.7; 95%CI, 1.7 to 6.4) and the low-volume of surgeons (OR, 95%CI, 1.2 to 3.6) CONCLUSIONS: Identification of these factors is of importance for the risk assessment of postoperative complications and the additional need of surgery. For patients with the above factors, especially those with combined risk factors, optimized operation scheme and high-volume surgeon should be considered to prevent or reduce the complications.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31499219

RESUMO

Neuropeptide Y (NPY) receptors are suggested to mediate the multi-physiological functions of NPY family peptides, such as food intake, in teleost fish. However, the structure and signaling of fish NPY receptors are yet to be fully elucidated. In this study, we report the cloning and characterization of two neuropeptide Y receptor subtypes, Y2 (NPY2R) and Y7 (NPY7R), in yellow croaker Larimichthys crocea (L. crocea) (LcNPY2R, LcNPY7R). The gene structure, pharmacological characterization, cell location, and tissue expression of these two receptors were explored. The phylogenetic results showed that LcNPY2R and LcNPY7R had typical G protein-coupled receptor profiles, associated with the Y2 subfamily, with coding sequences that are highly conserved in vertebrates. The expression of both LcNPY2R and LcNPY7R could be activated by LcNPY in HEK293 cells. However, truncated LcNPY18-36 was only able to activate LcNPY2R at the same level as full length LcNPY. Expression analysis revealed that LcNPY2R mRNA was predominantly expressed in the intestine and liver, whereas LcNPY7R was expressed in the stomach, which indicated that both receptors were related to the digestive system. Overall, our data establishes a molecular basis to determine the actions of LcNPY2R and LcNPY7R, which could be used to elucidate the conserved roles of these receptor-ligand pairs in vertebrates.

11.
Technol Cancer Res Treat ; 18: 1533033819874128, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31500518

RESUMO

Irreversible electroporation has raised great interest in the past decade as a means of destroying cancers in a way that does not involve heat. Irreversible electroporation is a novel ablation technology that uses short high-voltage electrical pulses to enhance the permeability of tumor cell membranes and generate irreversible nano-sized structural defects or pores, thus leading to cell death. Irreversible electroporation has many advantages over thermal therapies due to its nonthermal mechanism: (1) reduced risk of injury to surrounding organs and (2) no "heat-sink" effect due to nearby blood vessels. However, so far, it has been difficult for irreversible electroporation to completely ablate large tumors (eg, >3 cm in diameter). In order to overcome this problem, many preclinical and clinical studies have been performed to improve the efficacy of IRE in the treatment of large size of tumors through a chemical perspective. Due to the distribution of electric field, irreversible electroporation region, reversible electroporation region, and intact region can be found in the treatment of irreversible electroporation. Thus, 2 types of chemical enhancements of irreversible electroporation were discussed in the article, such as the reversible electroporation region enhanced and the irreversible electroporation region enhanced. Specifically, the state-of-the-art results regarding the following approaches that have the potential to be used in the enhancement of irreversible electroporation were systematically reviewed in the article, including (1) combination with cytotoxic drugs, (2) calcium electroporation, (3) modification of cell membrane, and (4) modification of the tumor cell microenvironment. In the end, we concluded with 4 issues that should be addressed in the future for improving irreversible electroporation further in a chemical way.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31476855

RESUMO

Self-supported aerogel composed of carbon nitride nanolayers can act as a bifunctional photocatalyst and show enhanced photoreduction and photooxidation performance due to the large surface areas and nitrogen vacancies. The carbon nitride aerogel can catalyze hydrogen evolution at a rate of nearly 4.2 mmol h-1 g-1 and oxidize benzyl alcohols with a high conversion efficiency and selectivity under milder conditions. Note that the activity of carbon nitride aerogel for photochemical alcohol oxidation shows outstanding performance compared with carbon nitride based photocatalysts. Both density functional theory and experimental results demonstrate that the introduction of nitrogen vacancies within the carbon nitride aerogel contributes to the formation of a crooked structure and enhanced adsorption of oxygen compared with a bulk sample.

13.
BMC Genomics ; 20(1): 708, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31510936

RESUMO

BACKGROUND: Bermudagrass (Cynodon dactylon L.) is an important turfgrass species with two types of stems, shoots and stolons. Despite their importance in determining the morphological variance and plasticity of bermudagrass, the intrinsic differences between stolons and shoots are poorly understood. RESULTS: In this study, we compared the proteomes of internode sections of shoots and stolons in the bermudagrass cultivar Yangjiang. The results indicated that 376 protein species were differentially accumulated in the two types of stems. Pathway enrichment analysis revealed that five and nine biochemical pathways were significantly enriched in stolons and shoots, respectively. Specifically, enzymes participating in starch synthesis all preferentially accumulated in stolons, whereas proteins involved in glycolysis and diverse transport processes showed relatively higher abundance in shoots. ADP-glucose pyrophosphorylase (AGPase) and pyruvate kinase (PK), which catalyze rate-limiting steps of starch synthesis and glycolysis, showed high expression levels and enzyme activity in stolons and shoots, respectively, in accordance with the different starch and soluble sugar contents of the two types of stems. CONCLUSIONS: Our study revealed the differences between the shoots and stolons of bermudagrass at the proteome level. The results not only expand our understanding of the specialization of stolons and shoots but also provide clues for the breeding of bermudagrass and other turfgrasses with different plant architectures.

14.
Zhen Ci Yan Jiu ; 44(6): 439-42, 2019 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-31368268

RESUMO

OBJECTIVE: To compare the clinical efficacy of soft-tissue relaxing needling and electroacupuncture (EA) in the treatment of knee osteoarthritis (KOA), so as to explore a new and more effective therapy for KOA. METHODS: Forty patients with KOA who met our diagnostic criteria were randomly and equally divided into acupuncture group and soft-tissue relaxing needling (relaxing-needling) group. EA (20 Hz, a tolerable strength and duration of 20 min) was applied to the unilateral Neixiyan(EX-LE5) and Waixiyan(EX-LE5), and manual acupuncture stimulation was applied to Heding(EX-LE2), Xuehai (SP10), Xiyangguan (GB33), Liangqiu(ST34), Yanglingquan(GB34) and Yinlingquan(SP9) on the affected side by using uniform reinforcing-reducing technique. In the relaxing-needling group, after identifying the tender point and nodule-like or stiff-strip-muscle spot at the affected limb by palpation, we used filiform needles to insert into them, then, made a longitudinal separation or point-like pricking. The visual analog scale (VAS) pain score, knee flexion activity (range of motion, ROM), and the knee osteoarthritis severity (Lequesne index, composed of daily living, walking distance and pain) were measured before and after the treatment. The therapeutic effect was assessed by consulting the Guiding Principles for Researching New Drugs of Traditional Chinese Medicine (2002) and Criteria for Diagnosis and Assessment of Therapeutic Effect of Syndromes or Illnesses of Traditional Chinese Medicine (1994). RESULTS: After the treatment, the VAS score and Lequesne index were significantly decreased in both acupuncture and relaxing-needling groups (P<0.001), and the ROM score was considerably increased in both groups in comparison with their own pre-treatment (P<0.001). The difference values of VAS score and Lequesne index between pre- and post-treatment were significantly higher in the relaxing-needling group than in the acupuncture group (P<0.05). Of the two 20 cases in the relaxing-needling and acupuncture groups, 8 and 3 experienced a remarkable improvement in their symptoms, 10 and 13 were effective, 2 and 4 failed, with the effective rate being 90.0% and 80.0%, respectively. No significant difference was found between the two groups in the difference value of ROM score and the effective rate (P>0.05).. CONCLUSION: Both relaxing-needling and EA therapies are comparable in the therapeutic effect for KOA, and the former is superior to the latter in reducing the joint pain and improving the knee joint locomotor function, thus being worthy of clinical application.


Assuntos
Eletroacupuntura , Osteoartrite do Joelho , Pontos de Acupuntura , Humanos , Medicina Tradicional Chinesa , Osteoartrite do Joelho/terapia , Resultado do Tratamento
15.
World J Gastroenterol ; 25(29): 3985-3995, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31413532

RESUMO

BACKGROUND: Hepatitis B is a major public health problem in China. Accurate liver injury assessment is essential for clinical evidence-based treatment. Liver biopsy is considered the gold standard method to stage liver disease, but it is not widely used in resource-limited settings. Therefore, non-invasive liquid biopsy tests are needed. AIM: To assess liver injury in hepatitis B patients using quantified cell free DNA combined with other serum biomarker as a liquid biopsy-based method. METHODS: A cohort of 663 subjects including 313 hepatitis B patients and 350 healthy controls were enrolled. Ultrasound-guided liver biopsies followed by histopathological assessments were performed for the 263 chronic hepatitis B patients to determine the degree of liver injury. Cell-free DNA was quantified using a novel duplex real-time polymerase chain reaction assay. RESULTS: Compared with healthy controls, patients with hepatitis B virus (HBV) infection had significantly higher plasma DNA, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, and HBV DNA levels (P < 0.01). Serum ALT, AST, bilirubin, and plasma DNA levels of patients with marked-severe inflammation were significantly higher than those with mild-moderate inflammation (P < 0.01). There was a statistically significant correlation between hepatocyte inflammation severity and serum bilirubin (R 2 = 0.673, P < 0.01) or plasma DNA (R 2 = 0.597, P < 0.01) levels. The areas under the curves of serum ALT, bilirubin, plasma DNA, and their combination to distinguish between patients with mild-moderate and marked-severe inflammation were 0.8059, 0.7910, 0.7921, and 0.9564, respectively. CONCLUSION: The combination of plasma DNA, serum ALT, and bilirubin could be a candidate liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients.

16.
Int J Nanomedicine ; 14: 6179-6195, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31447559

RESUMO

Background and purpose: Gambogic acid (GA) is a natural compound that exhibited a promising multi-target antitumor activity against several types of cancer. However, the clinical application of this drug is limited due to its poor solubility and low tumor cell-specific delivery. In this study, the monomeric and dimeric Cyclo (Arg-Gly-Asp) c(RGD) tumor targeting peptides (c(RGDfK) and E-[c(RGDfK)2]) were used to modify GA loaded nanostructured lipid carriers (NLC) to reduce the limitations associated with GA and improve its antitumor activity. Methods: GA-NLC was prepared by emulsification and solvent evaporation methods and the surface of the NLC was conjugated with the c(RGD) peptides via an amide bond. The formulations were characterized for particle size, morphology and zeta potential, encapsulation efficiency and drug loading. The in-vitro cytotoxicity and cell uptake studies were conducted using 4T1 cell. Furthermore, the in-vivo antitumor activity and bio-distribution study were performed on female BALB/c nude mice. Results: The c(RGD) peptides modified GA-NLC was successfully prepared with the particles size about 20 nm. The HPLC analysis, FT-IR and 1H-NMR spectra confirmed the successful conjugation of the peptides with the NLC. The in-vitro cytotoxicity study on 4T1 cells revealed that c(RGD) peptides modified GA-NLCs showed significantly higher cytotoxicity at 0.25 and 0.5 µg/mL as compared to unmodified GA-NLC. Furthermore, the cell uptake study demonstrated that better accumulation of E-[c(RGDfK)2] peptides modified NLC in 4T1 cell after 12 h incubation. Moreover, the in-vivo study showed that c(RGD)s functionalized GA-NLC exhibited better accumulation in tumor tissue and tumor growth inhibition. In contrast to the monomeric c(RGD) peptide, the dimeric c(RGD) peptide (E-[c(RGDfK)2]) conjugated GA-NLC showed the improved antitumor activity and tumor targeting ability of GA-NLC. Conclusion: These data provide further support for the potential clinical applications of E-[c(RGDfK)2]-GA-NLC in breast cancer therapy.

17.
Asia Pac J Clin Nutr ; 28(3): 520-532, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31464398

RESUMO

BACKGROUND AND OBJECTIVES: It is well known that dietary factors affect the development of cardiovascular disease. We evaluated the associations between carbohydrate intake and cardiovascular disease risk factors using data from the China Health and Nutrition Survey, 2009. METHODS AND STUDY DESIGN: A total of 6,648 Chinese adults aged 18-60 were divided into five groups based on carbohydrate intake (% of energy). Mixed-effect linear regression models were used to estimate the risk factors in relation to carbohydrate intake, and mixed-effect logistic regression models were used to assess the risk of cardiovascular disease. RESULTS: When age was adjusted, carbohydrate intake was negatively correlated with total cholesterol and triglycerides in men and total cholesterol and low-density lipoprotein cholesterol in women. However, there were positive associations of carbohydrate intake with waist circumference, body mass index, and blood pressure in women. After additional adjustment for urbanicity index, income, physical activity, education, alcohol and smoking, and dietary intake, the 5th quintile of carbohydrate intake reduced the risk for high low-density lipoprotein cholesterol in women (OR=0.73, 95% CI: 0.53, 0.99) compared with the 1st quintile. However, the top quintile of carbohydrate intake increased the risk for impaired glucose tolerance in men (OR=2.08, 95% CI: 1.04, 4.16) compared with the lowest quintile after adjusting for all confounders. CONCLUSIONS: Higher-carbohydrate diets may associate with risk factors for cardiovascular disease. Moderate carbohydrate intake is recommended for daily consumption. These results suggest that improving dietary patterns may be an important approach to the prevention of cardiovascular disease in Chinese adults.

18.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117466, 2019 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-31422340

RESUMO

Femtosecond time-resolved transient absorption spectroscopy is employed to investigate the ultrafast excited-state dynamics from the S2 state of dibenzofuran and the hydrogen bonding effect in protic and aprotic solvents. Following the excitation with 266 nm, the initial population on the S2 state couples to the S1 state via internal conversion on tens of picoseconds. Afterwards, vibrational energy relaxation (VER) with dozens of picoseconds is determined in all solvents and strongly depended on the polarities of aprotic solvents. And the rising species related with the hydrogen-bonded intermediate S⁎ state are observed just in ethanol and methanol with the higher Kamlet parameter α. Subsequently, the intersystem crossing from the S1 state to the triplet manifold is a main deactivation pathway with the decay time of ≫ns. All the relaxation processes exhibit solvent dependence, indicating that hydrogen bonding interaction between solute and solvent enhances VER and significantly influences on the excited state dynamics of dibenzofuran.

19.
Sheng Wu Gong Cheng Xue Bao ; 35(8): 1401-1410, 2019 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-31441611

RESUMO

Gene editing is a technique for modifying gene fragments. The novel gene editing technology focuses on the field of artificial nuclease cleavage technology, mainly ZFN technology, TALEN technology, CRISPR technology and base editing technology. The continuous improvement of gene editing technology has promoted the rapid development of agriculture, animal husbandry and biomedicine, but at the same time, technical defects and ethical controversy have brought enormous challenges to its own development. This article will briefly discuss the development and challenges of gene editing technology, as well as the views at home and abroad, and hope to inspire readers to recognize gene editing technology.


Assuntos
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Edição de Genes , Agricultura , Animais , Endonucleases
20.
Food Chem Toxicol ; 133: 110751, 2019 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-31390532

RESUMO

Chronic exposure to cadmium (Cd) causes remarkable damage to the liver and gastrointestinal tract. Previous studies have revealed that chlorogenic acid (CGA) could improve the intestinal barrier integrity for weaned rats. Hence, this study sought to investigate the protective effects of CGA from pure reagent and sunflower seed extract (SSE) on growth performance, antioxidant indicators, inflammatory biomarkers and intestinal barrier function in Cd treated rats. A total of 32 Sprague-Dawley female rats with similar weights were randomly allotted to four treatments: control, CdCl2 (6 mg/kg BW), co-treatment of Cd (6 mg/kg BW) and pure CGA (50 mg/kg BW), and co-treatment of Cd (6 mg/kg BW) and SSE (50 mg/kg BW) for 14 days. The data indicated that, CGA or SSE with Cd sequestration and good antioxidative ability decreased Cd absorption and accumulation in the jejunum and increased fecal Cd levels in Cd-exposed rats. Compared with the Cd group, co-treatment with CGA or SSE also alleviated inflammation, ameliorated the villus damage, reversed the disruption of tight junctions, and recovered weight gain of rats. These results suggest that CGA or SSE can protect the intestinal barrier, which is related to the alleviation of Cd-induced oxidative stress and growth decrease.

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