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J Biomed Nanotechnol ; 15(6): 1345-1353, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31072441


Gastric cancer is the fourth most common cancer worldwide and the third most common in Asia, with a high mortality. Photodynamic therapy (PDT) is a new treatment for cancer. With advantages of minimum invasiveness, small adverse side effects and high selectivity, PDT can be used as palliative treatment for patients with advanced gastric cancer. YLG I, also known as 2-(1 hexyloxyethyl)-2-devinyl porphin e6 trisodium salt (HCE6), is a recently developed photosensitizer. A previous study showed that HCE6 significantly inhibited the growth of QBC939 human cholangiocarcinoma cells. However, the effects and mechanisms of HCE6 on gastric cancer cell suppression are not known. In this study, we investigated the effects of HCE6 on the human gastric cancer cell line MKN45 and found that at the concentration of 2.0 mg/L, HCE6 almost completely killed MKN45 cells at a light intensity of 3.6 J/cm². RNAseq results confirmed that mitochondria and endoplasmic reticulum (ER)-mediated apoptosis was involved in the effects of HCE6 on cell death, and we also found that HCE6 induced chromosome conformational changes in the early phase of apoptosis. The results of our study help elucidate the molecular mechanisms underlying HCE6-mediated inhibition of gastric cancer cell growth and provide a theoretical basis and molecular targets for the treatment of gastric cancer.

Neoplasias Gástricas , Apoptose , Linhagem Celular Tumoral , Humanos , Fotoquimioterapia , Fármacos Fotossensibilizantes
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(2): 188-192, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-31106537


OBJECTIVE: To study the relationship between hypoxia and the hypoxia inducible factor-1α (HIF-1α) from lung cancer cells, to reveal the possible mechanism of brain metastases of lung cancer. METHODS: The hypoxia model of A549 lung cancer cells was established. After hypoxia culture of A549 cells for 0.5, 2, 4, 8, 12 and 24 h (normal oxygen culture at the same time point was set as the control group), the mass concentration of HIF-1α in A549 lung cancer cell culture medium were determined by ELISA. Transwell chamber was used to construct an in vitro blood brain barrier model, was treated with A549 lung cancer cell culture medium after different time points of hypoxia, Tran endothelial resistance (TER) change of blood-brain barrier model in instrument, to reflect the changes of blood-brain barrier permeability in vitro; A549 lung cancer cells in the culture medium were counted under Transwell room. A549 lung cancer cells with hypoxia at different time points injected into Wistar rats via tail vein, Western blot method was used to menstruate expression of tight junction associated protein Claudin-5 in the brain tissues, Evans blue to detect the change of blood brain barrier permeability in rats. RESULTS: Compared with the control group, the HIF-1α mass concentration in the cell culture solution of A549 increased, the in vitro blood-brain barrier model TER decreased, and the cell number of A549 that passed through transwell into the lower chamber increased (all P<0.05) after hypoxia 2 h, the above effect was most obvious when hypoxia 8 h (all P<0.01). After hypoxia 24 h, it was restored to the control group level. In the in vivo experiment of rats, compared with the control group, the mass percent of Evans blue in rat brain tissues increased after A549 cell culture solution with hypoxia 2 h was injected via caudal vein, meaning increased the permeability of rat blood brain barrier, while the expression of Claudin-5 protein in rat brain tissues decreased (all P<0.05). The effect was most obvious when A549 cell culture solution with hypoxia 8 h was injected into rat tail vein (P<0.01 ). Ejectionof hypoxia 24 h A549 cell culture solution yielded the same effects as those in the control group. CONCLUSION: Hypoxia can induce the increase of HIF-1α in lung cancer cells. The increase of HIF-1α results in the decrease of Claudin-5 expression and increase of blood-brain barrier permeability, leading to lung cancer cells metastasis into the brain.

Neoplasias Encefálicas/secundário , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Neoplasias Pulmonares/patologia , Células A549 , Animais , Hipóxia Celular , Humanos , Transplante de Neoplasias , Ratos , Ratos Wistar
Orthopedics ; 35(8): 702-8, 2012 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-22868596


The optimal treatment approach for the initial management of radial nerve palsy associated with humeral shaft fractures has yet to be conclusively determined. The authors performed a systematic review of the literature to identify studies that compared the outcomes after initial nonoperative and operative management for radial nerve palsy associated with acute humeral shaft fractures. A meta-analysis of the data from these studies was also performed to determine whether recovery from radial nerve palsy was more favorable in one approach compared with the other. The primary outcome was recovery from radial nerve palsy and the secondary outcome was complaints after treatment. Nine articles (1 prospective observational and 8 retrospective) were included in the meta-analyses. Operative management showed no improved recovery from radial nerve palsy compared with nonoperative management. Nonoperative management was associated with a decreased risk of complaints relative to operative management. Recovery from radial nerve palsy associated with acute humeral shaft fractures is not influenced by the initial management approach.

Fraturas do Úmero/terapia , Neuropatia Radial/terapia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Fraturas do Úmero/complicações , Fraturas do Úmero/cirurgia , Masculino , Pessoa de Meia-Idade , Neuropatia Radial/etiologia , Neuropatia Radial/cirurgia , Adulto Jovem
Zhongguo Gu Shang ; 25(10): 817-20, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23342795


OBJECTIVE: To explore clinical presentations and the operational opportunity of traumatic cervical disc herniation. METHODS: From June 2002 to June 2009,40 patients with traumatic cervical disc herniation were treated. There were 24 males and 16 females, with an average age of 43.2 years old ranging from 30 to 56 years. There were 36 patients with single intervertebral disc herniation and 4 patients with double. The injury level of those patients were at C3,4 in 16 cases, C4,5 in 10 cases, C5,6 in 12 cases and C6,7 in 6 cases. Among them, 18 patients showed spinal cord signal changes by MRI, 5 patients suffered from nothing but neck and shoulder pain, 8 patients with nerve root stimulation; 10 patients with spinal cord compression, and 17 patients had both nerve root stimulation and spinal cord compression symptoms. Conservative treatment were applied to 13 patients with neck and shoulder pain and nerve root stimulation, 5 cases of which were transferred to operation in case of poor effects, and Odom criteria were used to assess operational effects. Twenty-seven patients with spinal cord compression accepted operation from 1 to 27days after their trauma, 16 of which were operated in 5 days (early operational group with an JOA score of 11.3 +/- 2.8), other 11 cases were operated from 5 to 27 days (delayed operational group with an JOA score of 11.4 +/- 2.9 ), then functional assessment of spinal cord were assessed according to JOA criteria. RESULTS: Three patients who were transferred from conservative treatment recovered excellently according to Odom criteria and the other 2 were good at final followed-up. JOA score of early operational group increased from (11.3 +/- 2.8) to (15.3 +/- 1.8) one week after operation (P < 0.01), and (15.9 +/- 1.4) at final followed-up (P < 0.01). JOA score of delayed operational group increased from (11.4 +/- 2.9) to (14.0 +/- 2.6) one week after operation (P < 0.01), and (15.3 +/- 1.5) at final followed-up (P < 0.01). The recovery ratio of JOA score of early operational group were (74.6 +/- 16.8)% 1 week after operation,and increased to (85.6 +/- 13.6)% at final followed-up; while that of delayed operational group were (50.9 +/- 17.5)% and (68.2 +/- 21.5)%, and there were significant difference between early operational group and delayed operational group both at 1 week postoperation and final followup (P < 0.05). CONCLUSION: There are some difference in pathological segment and imaging manifestation between traumatic cervical disc herniation and cervical spondylosis. Early operation is favorable to the recovery of neurological function in patients with spinal cord compression.

Vértebras Cervicais/lesões , Deslocamento do Disco Intervertebral/cirurgia , Adulto , Vértebras Cervicais/cirurgia , Feminino , Humanos , Deslocamento do Disco Intervertebral/fisiopatologia , Masculino , Pessoa de Meia-Idade
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 33(12): 1148-53, 2008 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-19141984


OBJECTIVE: To evaluate the feasibility of endoscopic paravertebral approach surgery in the far lateral lumbar disc herniation. METHODS: Fifty sets of integral lumbar vertebral specimens were selected, and the anatomical data of lumbar intervertebral foramina and its adjacent structures were measured. Twenty specimens are randomly divided into a traditional group and an endoscopic group, then the traditional and endoscopic operations by paravertebral approach were used to dissect every strata of soft tissues in order to expose the intervertebral foramen. At last,the relationship between the intervertebral foramen and its adjacent structures was observed, and the 2 procedures were compared. RESULTS: The sagittal diameter of lumbar intervertebral foramina became shorter from the top to bottom,while the diameter of cross section of relative nerve roots became longer. The depth of the line which connected the middle point of the 2 adjacent transverse process roots and the anterior branch of lumber nerve root in L1,L3 and L5 was (1.03+/-0.30), (1.71+/-0.29), and (1.99+/-0.34) mm respectively, with the increasing tendency; the depth of L3 to L5 was mostly less than 2 cm. The distance of the middle points of the 2 adjacent transverse process roots from L1 to L5 was long enough, but the distance between L5 and S1 was only (10.14+/-1.71) mm. The surgery by paravertebral approach had a relative safe operation area,which was similar to a triangle. CONCLUSION: The technique of endoscopic surgery by paravertebral approach is feasible in the treatment of far lateral lumbar disc herniation. However, it is difficult to perform this surgery in the treatment of L5/S1 far lateral lumbar disc herniation, which is often conducted with endoscopy, after 18 to approximately 20 mm of the partial sacrum is eliminated by conventional method.

Discotomia/métodos , Endoscopia/métodos , Deslocamento do Disco Intervertebral/cirurgia , Vértebras Lombares/cirurgia , Adulto , Cadáver , Estudos de Viabilidade , Feminino , Humanos , Deslocamento do Disco Intervertebral/classificação , Masculino
Hunan Yi Ke Da Xue Xue Bao ; 27(1): 55-7, 2002 Feb 28.
Artigo em Chinês | MEDLINE | ID: mdl-12575237


OBJECTIVE: To explore the application of three dimensional(3D) CT reconstruction(3D-CT-R) in spinal stenosis and injury of the bone and joint. METHODS: Forty cases were examined with the X-ray film, CT scan and 3D-CT-R. To evaluate their diagnostic value, the images of the three methods were compared. RESULTS: X-ray films could show a visible shift of fracture pieces, but we were sometimes incapable of diagnosing fractures accurately from X-ray and, therefore, we couldn't diagnose fractures located at a special position; the CT scan could show the fractured place with a better result, but it required that doctors should have enough clinic experience, and should be familiar with local anatomy to avoid possible misdiagnosis. CONCLUSION: 3D-CT-R could be used in formulating a particular treatment scheme, and is an ideal examination method.

Fraturas do Quadril/diagnóstico por imagem , Luxações Articulares/diagnóstico por imagem , Estenose Espinal/diagnóstico por imagem , Adulto , Feminino , Humanos , Imagem Tridimensional , Masculino , Pessoa de Meia-Idade , Fraturas da Tíbia/diagnóstico por imagem , Tomografia Computadorizada por Raios X/métodos