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Gene ; 709: 17-24, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31102716


Angiopoietin-like protein 6, which is encoded by ANGPTL6 gene (also known as angiopoietin growth factor, AGF), has been extensively characterized with regard to its proposed functions as angiogenesis and energy metabolism. The present results showed the occurrence of alternative splicing by intron retention (IR) event in the bovine ANGPTL6 gene (bANGPTL6). By means of RT-PCR, TA clone and sequencing, we have shown that the bANGPTL6 gene has a splice variant generated by the retention of its partial intron 3. The computational analysis of the bANGPTL6 genomic sequence showed that its intron 3 has a high percentage of GC (62.31%) and a length of 199 nt, characteristics that have been associated with an IR event. The IR event does not interfere with the coding region as the bANGPTL6 prepropeptide is entirely coded in the third exon. Additionally, both the intronless (namely, bANGPTL6α) and intron-retaining (namely, bANGPTL6ß) ANGPTL6 transcripts are constitutively co-expressed in the bovine liver. Further, the relative expression level of different variants in liver was tested by both semi-RT-PCR and RT-qPCR methods. The results suggested bANGPTL6ß are significantly higher than bANGPTL6α. Overall, our findings will be helpful for studies on the molecular mechanism of IR events and the functions of ANGPTL6 gene. Specially, bANGPTL6ß gene probably contributes to a new target for treatment of obesity and obesity-related diseases.

Processamento Alternativo/genética , Proteínas Semelhantes a Angiopoietina/genética , Bovinos/genética , Íntrons/genética , Animais , Sequência de Bases , Clonagem Molecular , Feminino , Expressão Gênica , Fígado/metabolismo , Isoformas de Proteínas/genética
Stem Cell Reports ; 8(6): 1479-1487, 2017 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-28479306


Intestinal epithelial renewal is mediated by intestinal stem cells (ISCs) that exist in a state of neutral drift, wherein individual ISC lineages are regularly lost and born but ISC numbers remain constant. To test whether an active mechanism maintains stem cell pools in the Drosophila midgut, we performed partial ISC depletion. In contrast to the mouse intestine, Drosophila ISCs failed to repopulate the gut after partial depletion. Even when the midgut was challenged to regenerate by infection, ISCs retained normal proportions of asymmetric division and ISC pools did not increase. We discovered, however, that the loss of differentiated midgut enterocytes (ECs) slows when ISC division is suppressed and accelerates when ISC division increases. This plasticity in rates of EC turnover appears to facilitate epithelial homeostasis even after stem cell pools are compromised. Our study identifies unique behaviors of Drosophila midgut cells that maintain epithelial homeostasis.

Intestinos/citologia , Células-Tronco/metabolismo , Animais , Proliferação de Células , Células Cultivadas , Drosophila , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Enterócitos/citologia , Enterócitos/efeitos dos fármacos , Enterócitos/metabolismo , Canamicina/toxicidade , Pseudomonas/patogenicidade , Receptores Notch/genética , Receptores Notch/metabolismo , Regeneração/fisiologia , Transdução de Sinais , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
J Appl Genet ; 58(2): 241-247, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27924447


NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles. However, similar research on the bovine NOTCH1 gene is lacking. In this study, we detected the polymorphisms of the bovine NOTCH1 gene in a total of 448 individuals from Chinese Qinchuan cattle with DNA pooling, forced PCR-RFLP, and DNA sequencing methods. Five novel SNPs were identified within the NICD domain, and eight haplotypes comprising combinations of these five SNPs were studied as well. The association analysis of SNPs' effects with growth traits revealed that g.A48250G was significantly associated with body height, body weight, and height at hip cross, and that g.A49239C only showed significant associations with body height. This suggests that the NOTCH1 gene is a strong candidate gene that could be utilized as a promising marker in beef cattle breeding programs.

Bovinos/crescimento & desenvolvimento , Bovinos/genética , Receptor Notch1/genética , Animais , Estatura/genética , Peso Corporal/genética , Cruzamento , China , Feminino , Haplótipos , Humanos , Desequilíbrio de Ligação , Fenótipo , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA/veterinária
Mol Biol Rep ; 40(7): 4499-505, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23657594


Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an interesting novel adipocytokine with insulin-sensitizing effects. Some studies have suggested that vaspin could play an important role in the development of obesity and metabolic disorders. However, the tissue expression patterns in cattle and impact of vaspin gene variants on the growth traits has not been determined yet. Herein, we firstly investigated the tissue expression patterns of vaspin gene in new born and adult cattle. The results showed that vaspin was ubiquitously expressed in most tissues and strongly expressed in the heart, skeletal muscle and fat. Then, genetic variants within bovine vaspin gene were screened in 1235 individuals from five Chinese indigenous cattle breeds. Two novel mutations in coding region (NW_001494061: g.1124477 G>A and g.1118561 T>C) of bovine vaspin gene were identified using MspI PCR-RFLP and HhaI ACRS PCR-RFLP detection. Association analysis revealed both two mutations were significantly associated with bodyweight and chest girth at 24 months in cattle (P < 0.05). Therefore, the MspI and HhaI genetic variants of bovine vaspin gene were recommended as DNA markers related to growth traits through marker-assisted selection for genetics and breeding in cattle.

Perfilação da Expressão Gênica , Gordura Intra-Abdominal/metabolismo , Característica Quantitativa Herdável , Serpinas/genética , Alelos , Animais , Bovinos , Frequência do Gene , Ordem dos Genes , Estudos de Associação Genética , Genótipo , Mutação , Especificidade de Órgãos/genética , RNA Mensageiro/genética , Análise de Sequência de DNA
Ying Yong Sheng Tai Xue Bao ; 15(7): 1227-30, 2004 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-15506104


This paper studied the effect of fertilization on cucumber growth and yield, soil microbial biomass and soil enzyme activities in sunlight greenhouse in Loess Plateau. The results indicated that the growth and yield of cucumber were increased with application of manure and methane. Foliage application reduced the application rate of NP and manure. Fertilization had an obvious effect on the biological characteristics of soil in sunlight greenhouse. The number of bacteria was increased by manure and foliage fertilization, and that of fungi was increased by NP and methane fertilization but decreased by manure fertilization. Fertilization with manure, NP and methane also remarkably increased the number of actinomyces and the activities of urease, phosphatase and sucrase in soil. The activities of soil urease and phosphatase were increased by fertilization of manure, but little effect was found with fertilization of NP and methane.

Biomassa , Cucumis sativus/crescimento & desenvolvimento , Fertilizantes , Microbiologia do Solo , Solo/análise , Esterco , Metano , Sacarase/metabolismo , Luz Solar , Temperatura Ambiente , Urease/metabolismo
Ying Yong Sheng Tai Xue Bao ; 13(6): 672-4, 2002 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-12216390


Calcium, Magnesium, Copper, Zinc, Manganese and Iron are necessary elements for plant growth and important indicators for soil quality evaluation. After forestland being reclaimed, spatial distributions of soil elements would be affected by plowing, erosion-deposition-transportation, and soil element properties. In the initial stage of forestland being reclaimed (the first and second year), Cu, Zn, Mn, Fe, K, Ca, and Mg in different slope locations would be increased. After two years, these elements would be decreased because of soil erosion. After six years, Cu, Fe, K, and Mg would be decreased by 1.5-4.56%. SiO2 content on the upper slope would be increased as reclaimed year increased, but on the middle slope, SiO2 content would be decreased and Al element would be increased.

Solo/análise , Árvores , Cálcio/análise , Cobre/análise , Ferro/análise , Magnésio/análise , Manganês/análise , Zinco/análise