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1.
Huan Jing Ke Xue ; 41(4): 1666-1675, 2020 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608672

RESUMO

Combined with on-site water quality investigation and nirS gene high-throughput sequencing technology, the evolution characteristics and influencing factors of the denitrification community during the formation of spring thermal stratification in Zhoucun Reservoir were analyzed. The results show that the water body stratification gradually formed during this period, and the environmental factors (NO3-, NH4+, TN, TOC, BOD5, permanganate index, TP, Fe, and Mn) showed significant differences (P<0.01); nitrogen showed a significant decline process. High-throughput sequencing provided 8703 OTU, which were divided into three phyla and eight major genera, proteobacteria accounted for the largest proportion with 45.27%-78.90%. The α-diversity except for the Simpson index showed that the ACE index, Chao index, Shannon index, and coverage index showed significant differences (P<0.05). The principal coordinate analysis showed the denitrification community exhibited significant differences in the spring, which was consistent with adonis result (P<0.001); network analysis (OTU-OTU) showed that there were seven main modules in this period, including 316 edges of 131 nodes, and the proportion of positive correlation edges was 95.25%. Network analysis (OTU-environmental factors) showed that there were five modules in this period, including 329 edges of 140 nodes, and the proportion of positive correlation edges was 51.98%. Sixty-two indicator OTU and 28 keystone OTU were obtained based on the indicator OTU analysis and network analysis. RDA and mantel test analysis indicated that T, DO, NO3-, TN, TOC, BOD5, and TP were the main environmental factors driving the denitrifying bacterial community structure and the key denitrifying OTU evolution in spring. Our results will provide technical support for the migration and transformation of nitrogen in reservoir water and pollution control.

2.
Anim Sci J ; 91(1): e13320, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31845459

RESUMO

This study investigated how the effects of photoperiod on circadian clock genes in the skin contribute to the regulation of hair follicle cycling of Inner Mongolia white cashmere goats. Twenty-four female (non-pregnant) Inner Mongolia white cashmere goats aged 1 - 1.5 years old with similar live weights (mean, 20.36 ± 2.63 kg) were randomly allocated into two groups: a natural daily photoperiod group (NDPP group:10 - 16 hr Light, n = 12) and a short daily photoperiod group (SDPP group: 7 hr Light:17 hr Dark, n = 12). All goats were housed in individual pens from May 15 to October 15, 2015 and were fed the same diets. We detected the mRNA expression of brain and muscle arnt-like protein-1 (Bmal1), circadian locomotor output control kaput (Clock), cryptochrome-1 (Cry1), period homolog-1 (Per1) and Rev-erbα genes in the goat skin. ANOVA revealed a significant 24 hr (10:00 hr, 14:00 hr, 18:00 hr, 22:00 hr, 02:00 hr, 06:00 hr, 10:00 hr) variation between the SDPP and NDPP groups for three months (July, September, and October). In summary, the current results confirm that an intrinsic oscillating molecular clock exists in goat skin, and that the clock is important for potential timing mechanisms at the anagen phase of hair follicles, which would contribute to the regulation mechanisms of hair follicle cell proliferation.

3.
J Mater Chem B ; 7(48): 7736-7743, 2019 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-31746937

RESUMO

Supramolecular nano-drug delivery systems with stimuli-responsive features have attracted extensive attention in photodynamic therapy. In this work, a new kind of photo-controlled reversible two dimensional (2D) nanosheet was constructed by cucurbit[8]uril (CB[8])-mediated ternary complexation with lanthanide complexes, azobenzene quaternary ammonium salt and sodium dodecyl sulfonate, which exhibited rapid morphological transformation and high drug loading capacities. The constructed supramolecular secondary self-assembly system has become a very promising candidate as a drug nanocarrier.

4.
Asian-Australas J Anim Sci ; 32(4): 541-547, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30145869

RESUMO

OBJECTIVE: This study investigated the effects of photoperiod on nutrient digestibility, hair follicle (HF) activity and cashmere quality in Inner Mongolia white cashmere goats. METHODS: Twenty-four female (non-pregnant) Inner Mongolia white cashmere goats aged 1 to 1.5 years old with similar live weights (mean, 20.36±2.63 kg) were randomly allocated into two groups: a natural daily photoperiod group (NDPP group:10 to 16 h light, n = 12) and a short daily photoperiod group (SDPP group: 7 h light:17 h dark, n = 12). All the goats were housed in individual pens and fed the same diets from May 15 to October 15, 2015. The digestibility of crude protein (CP), dry matter (DM), and neutral detergent fiber (NDF) were measured in different months, along with secondary hair follicle (SHF) activity, concentration of melatonin (MEL), and cashmere quality. RESULTS: Although there was no significant difference in the live weights of goats between the SDPP and NDPP groups (p>0.05), the CP digestibility of goats in the SDPP group was significantly increased compared to the NDPP group in July, September, and October (p<0.05). For the DM and NDF digestibility of goats, a significant increase (p<0.05) was found during in September in the SDPP group. Furthermore, compared to the NDPP group, the SHF activity in July, the MEL concentration in July, and the cashmere fiber length and fiber weight in October were significantly increased in the SDPP group (p<0.05). CONCLUSION: The cashmere production of Inner Mongolia white cashmere goats was increased without obvious deleterious effects on the cashmere fibers in the SDPP group (metabolizable energy, 8.34 MJ/kg; CP, 11.16%; short daily photoperiod, 7 h light:17 h dark).

5.
Zhonghua Nan Ke Xue ; 24(7): 622-626, 2018 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-30173446

RESUMO

Objective: To explore the clinical selection and application of cell suspension examination (CSE) or histopathological technique (HPT) in detecting sperm in the testis tissue obtained by testicular sperm aspiration (TESA) in patients with non-obstructive azoospermia (NOA). METHODS: Totally, 1 006 NOA patients underwent TESA and their testis tissues were subjected to CSE or HPT for sperm detection. Based on the results of CSE, the testicular tissue samples were divided into groups A (with sperm, n = 567) and B (without sperm, n = 439) and the results were compared with those of HPT. RESULTS: HPT showed 508 cases with but 59 without sperm in group A, and 403 with and 36 without sperm in group B. The consistency rate of CSE with that of HPT was 90.56% (Kappa =0.809), and CSE exhibited a significantly higher rate of sperm detection than HPT (56.36% vs 54.08%, P=0.023). CONCLUSIONS: CSE combined with HPT for detecting sperm in the testis tissue of NOA patients undergoing diagnostic TESA helps clinical diagnosis and treatment. The results of CSE have a decisive significance for assisted reproductive therapy, while those of HPT may provide some definite etiological evidence for drug therapy or surgery.


Assuntos
Azoospermia , Recuperação Espermática , Testículo , Humanos , Masculino , Técnicas de Reprodução Assistida , Espermatozoides , Suspensões
6.
Huan Jing Ke Xue ; 39(4): 1962-1970, 2018 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965024

RESUMO

Seasonal asymmetric warming is one of the distinguishing features of global warming. However, if this feature is not considered in studying the effects of global changes on terrestrial ecosystems, it might probably cause misunderstanding of these studies. The releasing features of soil CO2 in Karst regions under various warming scenarios were simulated following a four-year continuous warming period using infrared radiators. A total of six treatments was arranged:no warming (ambient temp, CK); symmetric warming (ambient plus 2.0℃ full year, SW); and, lowly, moderately, highly, and extremely asymmetric warming (ambient plus 2.5℃/1.5℃, 3.0℃/1.0℃, 3.5℃/0.5℃, and 4.0℃/0℃ in the winter-spring/summer-autumn seasons, respectively, LAW, MAW, HAW, and EAW). The results showed that compared to CK, soil CO2 efflux in all the warming plots significantly increased by 0.26 µmol·(m2·s)-1, or 17.41%. In the winter-spring seasons, soil CO2 efflux in the warming treatments increased by 0.23 µmol·(m2·s)-1. The Q10 values ranged from 1.53 to 3.24 with an average of 2.23 under the scenario of warming up by 2.0℃. The warming-induced contribution of CO2 efflux in the summer-autumn seasons (80%) was obviously higher than that in the winter-spring seasons (20%) in the SW treatment, whereas the mean contribution in the summer-autumn seasons (46%) was closer to that in the winter-spring seasons (54%) in the asymmetric warming treatments. Both soil CO2 efflux and Q10 showed a tendency towards decrease with the increase in the asymmetry of warming under the five warming scenarios. The soil CO2 efflux in the SW treatment was significantly (P<0.05) higher than those in the MAW, HAW, and EAW treatments. The Q10 values in the summer-autumn seasons was larger than those in the winter-spring seasons under each warming treatment or across all warming treatments, which was probably related to soil water content, soil microbe, dissolved inorganic carbon, and vegetation growth. The results revealed that it may potentially overestimate the effects of global warming on soil CO2 releasing subject to symmetric warming.

7.
Huan Jing Ke Xue ; 39(7): 3391-3399, 2018 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-29962166

RESUMO

Elucidating the mechanisms of warming-induced stability for soil organic C is one of the keys for evaluating the tendency of soil C sources/sinks in projected global warming models. Organic C densities in soil, and soil physical and biochemical fractions, under different warming scenarios in the Karst Plateau were investigated following a 4-yr continuous warming using infrared radiators, via density fractionation and acid hydrolysis. Six treatments were arranged: no warming (ambient temp, CK); symmetric warming (ambient+2.0℃ full year); and lowly, moderately, highly, and extremely asymmetric warming (ambient+2.5℃/1.5℃, 3.0℃/1.0℃, 3.5℃/0.5℃, and 4.0℃/0℃ in winter-spring/summer-autumn seasons, respectively; LAW, MAW, HAW, and EAW). The moderately asymmetric warming was highly similar to a multi-year warming scenario in the study region. The results showed there were no significant differences in soil organic C densities in the surface layer (0-15 cm) among the warming treatments, with a range of 1.95 kg·m-2 to 2.02 kg·m-2, which is insignificantly different to the CK (1.94 kg·m-2). There were no significant differences in the C density of light and heavy fractions, and the recalcitrant heavy-fraction among the warming treatments, and between the warming and no warming treatments. The average recalcitrant C density of the light fraction in the warming treatments was 1.18 times higher than the CK, with a significantly higher recalcitrant C density of the light fraction in the symmetric warming, and lowly and moderately asymmetric warming treatments, compared to that of the CK. The recalcitrant C density and recalcitrant C index of the light fraction showed a tendency to decrease as the asymmetry of warming increased under the five warming scenarios. Warming had negligible effects on the organic C density in soil, and soil physical and biochemical fractions in the subsurface layer (15-30 cm). The results revealed that in the short-term, warming may increase the recalcitrance of non-protected C in the Karst Plateau soil. This is not necessarily an over- or underestimation of the effects of global warming on soil organic C density and the capacity of soil to protect C when subjected to symmetric warming, but may potentially overestimate the recalcitrance of organic C in the non-protected fraction of the surface layer (0-15 cm).

8.
Zhongguo Dang Dai Er Ke Za Zhi ; 19(12): 1243-1247, 2017 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-29237523

RESUMO

OBJECTIVE: To explore the value of urine gas chromatography-mass spectrometry (GC-MS) in the screening of children at risk of inherited metabolic diseases (IMD), and to identify the disease spectrum of IMD and the clinical characteristics of children with IMD. METHODS: The clinical data of 15 851 children at risk of IMD who underwent urine GC-MS in the Tianjin Children's Hospital between February 2012 and December 2016 were retrospectively analyzed. RESULTS: In the 15 851 children, 5 793 (36.55%) were detected to have metabolic disorders. A total of 117 (0.74%) children were confirmed to have IMD, including 77 cases of methylmalonic acidemia (65.8%). The clinical manifestations of confirmed cases in the neonatal period mainly included jaundice, metabolic acidosis, abnormal muscular tension, feeding difficulty, poor response, and lethargy or coma. The clinical manifestations of confirmed cases in the non-neonatal period mainly included delayed mental and motor development, metabolic acidosis, convulsion, recurrent vomiting, and anemia. CONCLUSIONS: GC-MS is an effective method for the screening for IMD in children at risk. Methylmalonic acidemia is the most common IMD. The clinical manifestations of IMD are different between the confirmed cases in the neonatal and non-neonatal periods.


Assuntos
Erros Inatos do Metabolismo/diagnóstico , Acidose/etiologia , Adolescente , Erros Inatos do Metabolismo dos Aminoácidos/complicações , Erros Inatos do Metabolismo dos Aminoácidos/diagnóstico , Criança , Pré-Escolar , Deficiências do Desenvolvimento/etiologia , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Lactente , Recém-Nascido , Masculino , Erros Inatos do Metabolismo/complicações , Estudos Retrospectivos , Risco
9.
Bosn J Basic Med Sci ; 17(4): 295-301, 2017 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-29055350

RESUMO

Previous studies suggested that oxidative stress is related to the onset and development of osteoporosis. Moreover, it was demonstrated that berberine has a protective effect against oxidative stress-induced injuries. In this study, we aimed to investigate the effect and mechanism of action of berberine on rats with induced osteoporosis. Sixty 8-week-old female Wistar rats were randomly divided into the following 6 groups: control saline-treated, osteoporosis saline-treated, 3 osteoporosis berberine-treated groups (Ber 5, 10, and 20 mg/kg/body weight, respectively), and osteoporosis alendronate-treated (ALD) group. Osteoporosis was induced by bilateral ovariectomy. All treatments were performed for 8 weeks. The bone mineral density (BMD), serum alkaline phosphatase (ALP), osteocalcin, calcium, phosphorus, superoxide dismutase (SOD), methylenedioxyamphetamine (MDA), and glutathione peroxidase (GSH-Px) level was determined in the rat femur tissue. The gene and protein expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) was analyzed by quantitative reverse transcription PCR and Western blot, respectively. The BMD, SOD and GSH⁃Px levels, and the expression of OPG were significantly lower in osteoporosis compared to control group (all p < 0.05). The serum levels of osteocalcin, ALP, and MDA, and the expression of RANKL were significantly higher in osteoporosis compared to control group (all p < 0.05). Berberine, especially the high doses of berberine, effectively increased SOD, GSH⁃Px, and OPG levels as well as decreased serum osteocalcin, ALP, MDA and RANKL levels in berberine-treated osteoporosis groups (all p < 0.05). To conclude, oxidative stress may promote the development of osteoporosis in rats through the RANK/RANKL/OPG pathway. The antioxidative effect of berberine reduces the development of osteoporosis in rats to some extent.


Assuntos
Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Berberina/farmacologia , Berberina/uso terapêutico , NF-kappa B/efeitos dos fármacos , Osteoporose/tratamento farmacológico , Osteoprotegerina/biossíntese , Estresse Oxidativo/efeitos dos fármacos , Ligante RANK/biossíntese , Animais , Peso Corporal , Densidade Óssea/efeitos dos fármacos , Feminino , Fêmur/patologia , Osteoporose/patologia , Osteoprotegerina/efeitos dos fármacos , Osteoprotegerina/genética , Ligante RANK/efeitos dos fármacos , Ligante RANK/genética , Ratos , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética
10.
Sci Rep ; 7(1): 5087, 2017 07 11.
Artigo em Inglês | MEDLINE | ID: mdl-28698595

RESUMO

Preeclampsia (PE) is a common pregnancy-related complication, and polymorphisms in angiotensinogen (AGT), angiotensin-converting enzyme (ACE), and angiotensin II type 1 receptor (AT1R) are believed to contribute to PE development. We implemented a hybrid study to investigate the influence of maternal and fetal ACE I/D, ACE G2350A, AGT M235T, AGT T174M, and AT1R A1166C polymorphisms on PE in Han Chinese women. Polymorphisms were genotyped in 1,488 subjects (256 patients experiencing PE, along with their fetuses and partners, and 360 normotensive controls with their fetuses). Transmission disequilibrium tests revealed that ACE I/D (P = 0.041), ACE G2350A (P = 0.035), and AT1R A1166C (P = 0.018) were associated with maternal PE. The log-linear analyses revealed that mothers whose offspring carried the MM genotype of AGT M235T had a higher risk of PE (OR = 1.54, P = 0.010), whereas mothers whose offspring carried the II genotype of ACE I/D or the GG genotype of ACE G2350A had a reduced risk (OR = 0.58, P = 0.039; OR = 0.47, P = 0.045, respectively). Our findings demonstrate that fetal ACE I/D, ACE G2350A, AGT M235T, and AT1R A1166C polymorphisms may play significant roles in PE development among pregnant Han Chinese women.


Assuntos
Feto/metabolismo , Pais , Polimorfismo Genético , Pré-Eclâmpsia/genética , Sistema Renina-Angiotensina/genética , Adulto , Estudos de Casos e Controles , Feminino , Interação Gene-Ambiente , Estudos de Associação Genética , Loci Gênicos , Predisposição Genética para Doença , Humanos , Funções Verossimilhança , Polimorfismo de Nucleotídeo Único/genética , Gravidez , Fatores de Risco
11.
Chin Med Sci J ; 31(3): 192-195, 2016 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-27733228

RESUMO

Gastric cancer is the result of multiple risk factors, including environmental factors, genetic factors and the interaction between them. The environmental factors mainly include dietary, Helicobacter pylori infection and family history of gastric cancer. Genetic factors mainly refer to the susceptible genes that cause epigenetic alterations in oncogenes, tumor suppress genes, cell cycle regulators, DNA repair genes and signaling molecules. This paper summarizes the susceptible genes of gastric cancer and explores the genetic basis of it.


Assuntos
Neoplasias Gástricas/genética , Inibidor de Quinase Dependente de Ciclina p15/genética , Genes Supressores de Tumor , Genes p16 , Humanos , Oncogenes , Neoplasias Gástricas/etiologia
12.
Taiwan J Obstet Gynecol ; 55(4): 507-11, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27590372

RESUMO

OBJECTIVE: To evaluate the impact of different hemostasis methods on ovarian reserve in laparoscopic cystectomy in treatment of ovarian endometrioma for the long-term. MATERIALS AND METHODS: A total of 207 patients with ovarian endometrioma, aged from 18 years to 45 years, were randomized into three groups: Group A (69 patients) treated by bipolar electrocoagulation hemostasis in laparoscopic cystectomy for ovarian endometrioma; Group B (69 patients) with ultrasound scalpel hemostasis; and Group C (69 patients) with suture technique hemostasis. The follicle-stimulating hormone (FSH), anti-Mullerian hormone (AMH), antral follicle count (AFC), and peak systolic velocity (PSV) were observed and compared at the 3(rd) day of the 1(st), 3(rd), 6(th), and 12(th) menstrual cycle after surgery. RESULTS: (1) A total of 13 out of 207 patients failed; four in Group A, five in Group B, and four in Group C. There was no statistically significant difference between groups (p > 0.05). The failure rate was the highest during the 3(rd) month in the follow up (10 cases). (2) FSH: at the 1(st) month, 3(rd) month, 6(th) month, and 12(th) month follow up, FSH was higher in Group A and Group B than in Group C (p < 0.05). (3) AMH: AMH was significantly lower in Group A and Group B than in Group C (p < 0.05) during the same period. (4) AFC: no difference of AFC was observed at the 1(st) month and 3(rd) month (p > 0.05), whereas at the 6(th) month and 12(th) month, AFC in Group C was obviously higher than that in Group A and Group B (p < 0.05). (5) PSV: at the 1(st) month, 3(rd) month, 6(th) month, and 12(th) month follow up, PSV was significantly lower in Group A and in Group B than in Group C (p < 0.05). CONCLUSION: Ultrasonic scalpel or bipolar electrocoagulation hemostasis applied to laparoscopic cystectomy is associated with a significant reduction of ovarian reserve. Electrocoagulation of hemostasis should be used with caution.


Assuntos
Endometriose/cirurgia , Procedimentos Cirúrgicos em Ginecologia/métodos , Hemostasia Cirúrgica/métodos , Doenças Ovarianas/cirurgia , Reserva Ovariana/fisiologia , Adolescente , Adulto , Hormônio Antimülleriano/sangue , Eletrocoagulação , Endometriose/fisiopatologia , Feminino , Hormônio Foliculoestimulante/sangue , Humanos , Laparoscopia , Ciclo Menstrual/sangue , Pessoa de Meia-Idade , Doenças Ovarianas/fisiopatologia , Folículo Ovariano , Ovário/fisiopatologia , Ovário/cirurgia , Período Pós-Operatório , Técnicas de Sutura , Fatores de Tempo , Ultrassonografia , Adulto Jovem
13.
J Hazard Mater ; 318: 443-451, 2016 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-27450336

RESUMO

In this study, we investigated the effects of a symbiotic bacterium and phosphate (PO4(3-)) nutrition on the toxicity and metabolism of arsenate (As(V)) in Dunaliella salina. The bacterium was identified as Alteromonas macleodii based on analysis of its 16S rRNA gene sequence. When no As(V) was added, A. macleodii significantly enhanced the growth of D. salina, irrespective of PO4(3-) nutrition levels, but this effect was reversed after As(V)+PO4(3-) treatment (1.12mgL(-1)) for 3 days. Arsenic (As) absorption by the non-axenic D. salina was significantly higher than that by its axenic counterpart during incubation with 1.12mgL(-1) PO4(3-). However, when the culture was treated with 0.112mgL(-1) PO4(3-), As(V) reduction and its subsequent arsenite (As(III)) excretion by non-axenic D. salina were remarkably enhanced, which, in turn, contributed to lower As absorption in non-axenic algal cells from days 7 to 9. Moreover, dimethylarsinic acid was synthesized by D. salina alone, and the rates of its production and excretion were accelerated when the PO4(3-) concentration was 0.112mgL(-1). Our data demonstrate that A. macleodii strongly affected As toxicity, uptake, and speciation in D. salina, and these impacts were mediated by PO4(3-) in the cultures.


Assuntos
Alteromonas/metabolismo , Arseniatos/metabolismo , Clorófitas/metabolismo , Alteromonas/química , Arseniatos/toxicidade , Biotransformação , Clorófitas/classificação , Clorófitas/efeitos dos fármacos , Meios de Cultura , Fosfatos/farmacologia , Simbiose
14.
Zhonghua Nan Ke Xue ; 22(12): 1095-1098, 2016 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-29282914

RESUMO

Objective: To investigate the value of serum inhibin B (InhB) in predicting the results of testicular sperm extraction (TESE) in patients with azoospermia. METHODS: This study included 191 cases of obstructive azoospermia (OA), 360 cases of nonobstructive azoospermia (NOA), and 100 male controls with normal semen parameters. According to whether sperm was found in TESE, the NOA patients were divided into a sperm-absence group (TESE-, n=127) and a sperm-presence group (TESE+, n=233). Blood samples were collected from the subjects between 8:00 and 10:00 am for measurement of the levels of serum InhB. The specificity and sensitivity of serum InhB predicting TESE outcome were analyzed with receiver operating characteristics curve (ROC). RESULTS: The serum InhB level was significantly lower in the TESE- group (ï¼»19.7±34.8ï¼½ pg/ml) than in the OA (ï¼»106.8±66.2ï¼½ pg/ml), TESE+ (ï¼»98.2±62.4ï¼½ pg/ml) and normal control group (ï¼»108.3±65.0ï¼½ pg/ml) (P<0.01), but there were no statistically significant differences among the latter three groups (P>0.05). Analysis of ROC showed that the best cut-off value of serum InhB was 19.1 pg/ml. Under the cut-off value, AUCROC was 0.88, specificity 84.2%, sensitivity 90.1%, and accuracy 88.1%. CONCLUSIONS: The serum InhB level is a good and non-invasively obtained index of spermatogenesis and should be measured for predicting the results of TESE.


Assuntos
Azoospermia/sangue , Inibinas/sangue , Recuperação Espermática , Espermatogênese , Humanos , Masculino , Curva ROC , Sêmen , Sensibilidade e Especificidade , Espermatozoides/citologia , Testículo
15.
Huan Jing Ke Xue ; 37(11): 4187-4193, 2016 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964669

RESUMO

In order to explore the trend and driving factors of nitrogen in the early stage of the thermal stratification in Zhoucun reservoir, the water quality indicators in main reservoir area of Zhoucun reservoir were monitored regularly form February to April (2016). Meanwhile, the fresh water and surface sediments in the sampling point were collected to study the effect of water and sediment denitrification by simulation in laboratory. The results showed that: the concentration of TN decreased from (2.28±0.09) mg·L-1 to (1.08±0.09) mg·L-1, the concentration of nitrate nitrogen decreased from (1.66±0.09) mg·L-1 to (0.25±0.06) mg·L-1, whereas the concentration of ammonia nitrogen and nitrite showed little change in the early thermal stratification of Zhoucun reservoir. At the same time, chlorophyll was not significantly increased, so the algae had little effect on nitrogen; the decreasing nitrogen was mainly due to the aerobic denitrification. Gradually increased temperature,the changes of DO and pH and the composition of organic matter (low molecular weight) were beneficial to the growth of aerobic denitrifying bacteria. Furthermore, the number of bacteria increased from 1.06×105 cfu·L-1 to 8.33×106 cfu·L-1, which enhanced the denitrification of the reservoir; Meanwhile, in the simulation experiments, the TN removal rates of water and water-sediment systems reached 0.7 mg and 3.3 mg, respectively. Water and sediment denitrification ratio was generally 1:4, and the aerobic denitrification of surface sediment was an important factor in the removal of nitrogen in reservoir.

16.
Huan Jing Ke Xue ; 37(9): 3394-3401, 2016 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964773

RESUMO

The fluorescent components were examined using excitation emission matrix fluorescence spectroscopy-parallel factor analysis technique for samples collected in August, 2015 from Zhouncun Reservoir. Principal component analysis was used to study the main factors and their relative contributions to DOM. Three fluorescent components were identified by PARAFAC, including fulvic-like component(C1:260,350/420 nm), protein-like(C2:280/360 nm) and humic-like (C3:270,390/530 nm) which showed the same source. The even spatial distribution of each component, higher total fluorescence intensity in storage port, high fluorescence index, high biological index, low humification index and the freshness index which was close to one showed that the DOM had a strong autochthonous contribution. The results of PCA showed that the autochthonous contribution reached 70.86%, and those three components of Zhoucun Reservoir could be connected with aph(440) by nonlinear multiple regression which means we can use the three-dimensional fluorescence spectrum results of DOM to control the pollution sources and indicate the eutrophication degree of Zhoucun Reservoir.


Assuntos
Eutrofização , Poluição da Água/análise , China , Análise Fatorial , Análise de Componente Principal , Estações do Ano , Espectrometria de Fluorescência
17.
Huan Jing Ke Xue ; 37(9): 3438-3446, 2016 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964778

RESUMO

Algae-bacteria consortia may be potentially applied in wastewater treatment and environment remediation. In this study, in order to investigate effects of a symbiotic bacterium on the accumulation and transformation of arsenate[As(Ⅴ)] by Chlorella salina, we used batch cultures to determine the uptake, adsorption and transformation of As by axenic and non-axenic C. salina exposed to 0-750 µg·L-1 As(Ⅴ) for 7 d. The symbiotic bacterium of C. salina was confirmed to be Halomonas sp. after isolation, cultivation and 16S rRNA identification. The bacterial presence markedly increased the adsorption of As in C. salina, but it markedly reduced the absorption and the toxic effect of As(Ⅴ). Arsenate was the major arsenic species in the cells of axenic and non-axenic C. salina. The proportion of arsenite[As(Ⅲ)] was 8.99%-11.52% in the axenic microalga whereas a small quantity of monomethylarsonous acid (MMA) and dimethylarsinous acid (DMA) (0.02%-0.04%) were detected in the non-axenic counterpart. As(Ⅲ) dominated the As speciation in the bacterial culture and the percentage of As(Ⅴ) was 7.59%-26.80%, indicating that this symbiotic bacterium had a strong As(Ⅴ) reducing ability. The As removal rate (19.81%-41.08%) by non-axenic C. salina was higher than the bacterium alone (5.14%-14.62%) and axenic C. salina (14.98%-21.08%) after 7 d As(Ⅴ) exposure. The symbiotic Halomonas sp. promoted the accumulation of As by C. salina, indicating that algae-bacteria consortia might enhance the bioremediation of As contaminated water.


Assuntos
Arseniatos/metabolismo , Chlorella/metabolismo , Chlorella/microbiologia , Halomonas/fisiologia , Simbiose , Arsênico , Biodegradação Ambiental , Halomonas/classificação , RNA Ribossômico 16S
18.
Huan Jing Ke Xue ; 37(12): 4577-4585, 2016 Dec 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965297

RESUMO

The differences in the resource and characteristics of DOM between Zhoucun Reservoir and Jinpen Reservoir were studied by fluorescence ultraviolet-visible (UV-vis) and excitation-emission matrix spectra combined with parallel analysis (EEMs-PARAFAC). The results showed that three fluorescent components were identified by PARAFAC model in Zhoucun Reservoir and Jinpen Reservoir, including long wave humus-like component (C1: 350/460 nm), visible fulvic-like (C2: 335/410 nm) and protein-like (C3: 260,285/360 nm). However, the fluorescence intensity and the relative proportions of DOM exhibited significant difference (P<0.01) in two reservoirs. Moreover, the concentration of protein-like component in Zhoucun Reservoir was higher than that in Jinpen Reservoir, while the concentration of humus-like component presented the opposite trend. Based on the comparison of fluorescence index, biological index, humification index and the freshness index, the DOM of Zhoucun Reservoir where the land-use type was cultivated, livestock and residential, the internal pollution of sediments was serious, indicating a strong autochthonous component and aquatic bacterial origin, whereas the DOM of Jinpen Reservoir dominated by forest system had a higher terrigenous contribution in winter. From all the results, hydrological conditions of the reservoir and the characteristics of coastal ecological environment were important factors to influence the DOM sources and characteristics.


Assuntos
Compostos Orgânicos/análise , Estações do Ano , Poluentes Químicos da Água/análise , Abastecimento de Água , Agricultura , Bactérias , China , Florestas , Solo , Espectrometria de Fluorescência
19.
J Huazhong Univ Sci Technolog Med Sci ; 35(6): 801-807, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26670428

RESUMO

The prevalence of hypertensive disorders in China was much higher than that in the United States. Considering the large population with wide geographic area of China, we aimed to add more information regarding the risk factors for hypertensive disorders of pregnancy. A case-control study was performed on 373 hypertensive cases and 507 normotensive controls. Participants were recruited from 2008 to 2014 in Yichang Maternal and Child Health Care Center in Hubei province and Anyang Maternal and Child Health Care Hospital in Henan province, China. Socio-demographic factors, family- related factors, pregnancy-associated factors, factors related to daily life behaviors and psychosocial factors were investigated with respect to hypertensive disorders in pregnancy through well-designed questionnaire. Chi-square test, t-test, univariate logistic regression analysis, and multivariate logistic regression analysis were used to find the possible risk factors behind hypertensive disorders in pregnancy. The results showed that family history of cardiovascular diseases (OR=6.18, 95% CI, 2.37 to 16.14), history of pregnancy-induced hypertension (OR=16.64, 95% CI, 5.74 to 48.22), low maternal educational level (OR=2.81, 95% CI, 1.30 to 6.04), and poor relationship with their parents-in-law (OR=3.44, 95% CI, 1.55 to 7.59) had statistically significant associations with hypertensive disorders in pregnancy through multivariate logistic regression analysis. Increased maternal age, increased pre-pregnancy body mass index, living in rural area, low paternal education level, family history of hypertension, passive smoking one year before and/or in pregnancy, and poor sleeping quality were significantly associated with hypertensive disorders in pregnancy from univariate logistic regression analysis while the associations became uncertain when they were entered for multivariate logistic regression analysis. It was concluded that family history of cardiovascular diseases, history of pregnancy-induced hypertension, low maternal educational level, and poor relationship with their parents-in-law were independent risk factors for hypertensive disorders among Chinese pregnant women.


Assuntos
Hipertensão/epidemiologia , Complicações Cardiovasculares na Gravidez/epidemiologia , Adulto , China/epidemiologia , Feminino , Humanos , Gravidez , Fatores de Risco , Adulto Jovem
20.
Medicine (Baltimore) ; 94(40): e1681, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26448013

RESUMO

Malaria is highly endemic in Yunnan Province, China, with the incidence of malaria being highest along the Sino-Burmese border. The aim of our study was to determine whether genetic polymorphisms are associated with the prevalence of malaria among Chinese residents of the Sino-Burmese border region. Fourteen otherwise healthy people with glucose-6-phosphate dehydrogenase (G6PD) deficiency, 50 malaria patients, and 67 healthy control subjects were included in our cross-sectional study. We analyzed the frequency of the G3093T and T520C single-nucleotide polymorphisms (SNPs) of CR1. Logistic regression was used to calculate the prevalence odds ratio (POR) and 95% confidence interval (CI) of malaria for the T520C SNP of CR1 and SNPs of G6PD, IL-4, IL-4R, IL-1A, NOS, CD40LG, TNF, and LUC7L. The frequency of the 3093T/3093T genotype of CR1 in the malaria group (0.16) was significantly higher than that in the control group (0.045, P < 0.05), and significantly lower than that in the G6PD deficiency group (0.43, P < 0.01). The frequency of the 520T/520T genotype of CR1 was significantly higher in the malaria patients (0.78) than that in the control group (0.67, P < 0.05) and G6PD-deficiency group (0.36, P < 0.05). The T allele of the T520C variant of CR1 was significantly associated with the prevalence of malaria (POR: 1.460; 95% CI: 0.703-3.034). Polymorphisms of G6PD did not significantly influence the prevalence malaria (P > 0.05). A GTGTGTC haplotype consisting of IL-1A (rs17561), IL-4 (rs2243250), TNF (rs1800750), IL-4R (rs1805015), NOS (rs8078340), CD40LG (rs1126535), and LUC7L (rs1211375) was significantly associated with the prevalence of malaria (POR: 1.822, 95% CI: 0.998-3.324). The 3093G/3093G and 520T/520T genotypes are the predominant genetic variants of CR1 among Chinese residents near the Sino-Burmese border, and the T allele of T520C is associated with the prevalence of malaria in this region. Although G6PD deficiency does not protect against malaria, it may diminish the association between malaria and the CR1 polymorphisms in this population. The GTGTGTC haplotype is also associated with the prevalence of malaria in this region.


Assuntos
Citocinas/genética , Deficiência de Glucosefosfato Desidrogenase/genética , Malária/epidemiologia , Óxido Nítrico Sintase/genética , Proteínas de Ligação a RNA/genética , Receptores de Complemento 3b/genética , Adulto , Alelos , China/epidemiologia , Estudos Transversais , Feminino , Genótipo , Deficiência de Glucosefosfato Desidrogenase/epidemiologia , Humanos , Incidência , Interleucinas/genética , Malária/genética , Masculino , Mianmar/epidemiologia , Razão de Chances , Reação em Cadeia da Polimerase , Polimorfismo de Nucleotídeo Único , Prevalência , Fator de Necrose Tumoral alfa/genética
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