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1.
J Hazard Mater ; 401: 123295, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32659574

RESUMO

Due to poor mass transfer performance and high energy consumption of the traditional electrochemical flow-by mode, this study developed a high-efficiency electrochemical oxidation system in flow-through mode based on three-dimensional macroporous enhanced TiO2 nanotube array/SnO2-Sb (MP-Ti-ENTA/SnO2-Sb) anode. The effects of initial pH, current density and flow rate on the COD degradation of reverse osmosis concentrates (ROCs) from reclaimed wastewater plant were investigated. Besides, the energy efficiency, biodegradability and acute biotoxicity were studied during electrochemical flow-through process. Compared with the flow-by mode, the flow-through mode based on the MP-Ti-ENTA/SnO2-Sb anode had a COD removal rate of 0.38 mg min-1 (current density: 5 mA cm-2) and an electrical efficiency per order (EE/O) of 5.3 kW h m-3. The three-dimensional fluorescence spectrum showed that the fulvic acids, humic acids and soluble microbial metabolites of ROCs could be effectively removed by the flow-through anode. In addition, the luminescence inhibition rate of the effluent was 22.4 %, indicating that the acute biotoxicity was reduced by more than 40 %. The electrochemical flow-through process of ROCs treatment required relatively low energy consumption without extra chemical agent addition, showing a broader application prospect.

2.
Dis Markers ; 2020: 3461315, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33014185

RESUMO

Although intestinal microbial dysbiosis was confirmed to be associated with many chronic diseases and health status through complicated interaction with the host, the effect on gastric cancer was less studied. In this study, we sequenced the 16S rRNA and 18S rRNA genes of fecal bacteria and fungi, respectively, in 134 gastric cancer patients and 58 healthy controls matched by age and gender. Propensity score matching (PSM) was adopted for adjusting diet habits and lifestyle, and 44 patients and 44 healthy controls (matching population) were enrolled. Serum antibody to H. pylori and metabolites of the matching population were detected. The positive rates of antibody to H. pylori between the patients and the control group did not reach the statistical difference. LEfSe analysis indicated that bacteria were more stable than fungi when adjusting diet and lifestyle. Veillonella, Megasphaera, and Prevotella 7 genus and Streptococcus salivarius subsp. Salivarius, Bifidobacterium dentium, and Lactobacillus salivarius species in bacteria were related to the risk of gastric cancer and showed a good diagnostic value in distinguishing the patients from healthy controls. Streptococcus mitis showed a risk effect for gastric cancer; however, the effect turned into be protective after PSM. Serum L-alanine, L-threonine, and methionol were positively associated with Veillonella and Streptococcus and several fungi genus. Overall, our findings indicated that fecal microbiome constitution alteration may be associated with gastric cancer through influencing the amino acid metabolism.

3.
Carcinogenesis ; 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33095847

RESUMO

A substantial fraction of transcripts are known as long noncoding RNAs (lncRNAs), and these transcripts play pivotal roles in the development of cancer. However, little information has been published regarding the functions of lncRNAs in oesophageal squamous cell carcinoma (ESCC) and the underlying mechanisms. In our previous studies, we demonstrated that small nucleolar RNA host gene 5 (SNHG5), a known lncRNA, is dysregulated in gastric cancer (GC). In this study, we explored the expression and function of SNHG5 in development of ESCC. SNHG5 was found to be downregulated in human ESCC tissues and cell lines, and this downregulation was associated with cancer progression, clinical outcomes and survival rates of ESCC patients. Furthermore, we also found that overexpression of SNHG5 significantly inhibited the proliferation, migration, and invasion of ESCC cells in vivo and in vitro. Notably, we found that metastasis-associated protein 2 (MTA2) was pulled down by SNHG5 in ESCC cells using RNA pulldown assay. We also found that SNHG5 reversed the EMT by interacting with MTA2. In addition, overexpression of SNHG5 downregulated the transcription of MTA2 and caused its ubiquitin-mediated degradation. Thus, overexpression of MTA2 partially abrogated the effect of SNHG5 in ESCC cell lines. Furthermore, we found that MTA2 mRNA expression was significantly elevated in ESCC specimens, and a negative correlation between SNHG5 and MTA2 expression was detected. Overall, this study demonstrated, for the first time, that SNHG5-regulated MTA2 functions as an important player in the progression of ESCC and provide a new potential therapeutic strategy for ESCC.

5.
Biomater Sci ; 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32966381

RESUMO

Nanomedicine has attracted growing attention due to its designability and functionality, as well as its excellent pharmacokinetics with limited side effects, and recently, combined therapies have become desirable as they can obtain enhanced therapeutic efficacy by using nanomedicine. Herein, we have reported a functional drug delivery system with a dual response to temperature and reactive oxygen species to efficiently eliminate pancreatic cancer cells in a combined therapy strategy. Functional micelles with camptothecin (CPT) in the core and indocyanine green (ICG) on the surface could effectively accumulate in tumor sites through the EPR effect. The ROS in the tumor microenvironment trigger the conversion of an amino-based copolymer to a carboxy based copolymer, releasing the loaded ICG to reduce the size of the micelles with high penetration in tumor tissue. On the one hand, under 808 nm light irradiation, the micelles will produce the heat to kill tumor cells via photothermal therapy. On the other hand, the generated heat could further trigger the transition of a copolymer from a hydrophobic to a hydrophilic state, releasing the loaded CPT into the deep tumor cells to achieve chemotherapy. The in vitro and in vivo experiments revealed that this combined therapy could combat pancreatic cancer cells with an enhanced therapeutic effect.

6.
J Cell Mol Med ; 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32969198

RESUMO

The persistent transactivation of epidermal growth factor receptor (EGFR) causes subsequent activation of the TGF-ß/Smad3 pathway, which is closely associated with fibrosis and cell proliferation in diabetic nephropathy (DN), but the exact mechanism of persistent EGFR transactivation in DN remains unclear. ARAP1, a susceptibility gene for type 2 diabetes, can regulate the endocytosis and ubiquitination of membrane receptors, but the effect of ARAP1 and its natural antisense long non-coding RNA (lncRNA), ARAP1-AS2, on the ubiquitination of EGFR in DN is not clear. In this study, we verified that the expression of ARAP1 and ARAP1-AS2 was significantly up-regulated in high glucose-induced human proximal tubular epithelial cells (HK-2 cells). Moreover, we found that overexpression or knockdown of ARAP1-AS2 could regulate fibrosis and HK-2 cell proliferation through EGFR/TGF-ß/Smad3 signalling. RNA pulldown assays revealed that ARAP1-AS2 directly interacts with ARAP1. Coimmunoprecipitation, dual-immunofluorescence and ubiquitination assays showed that ARAP1 may maintain persistent EGFR activation by reducing EGFR ubiquitination through competing with Cbl for CIN85 binding. Taken together, our results suggest that the lncRNA ARAP1-AS2 may promote high glucose-induced proximal tubular cell injury via persistent EGFR/TGF-ß/Smad3 pathway activation by interacting with ARAP1.

8.
J Fluoresc ; 2020 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-32780262

RESUMO

Carbon dots (CDs) with different doping elements were successfully synthesized via a simple hydrothermal strategy. 3-amino-4-chlorophenylboronic acid, 3-aminobenzeneboronic acid, aniline, and benzene were used as precursors, respectively. The B/N co-doping CDs (BNCDs) derived from 3-aminobenzeneboronic acid show brightest fluorescence among the CDs products with quantum yield at 0.15. The fluorescence of BNCDs exhibits good photostability and excitation-independent emission behavior. The bright blue emission of BNCDs can be quenched by serine, which is a kind of neutral aliphatic amino acid containing hyroxyl groups with polarity. It is possibly due to the molecular collision between excited state of BNCDs and the ground state of serine. BNCDs can be served as fluorophore probe for the assay of serine based on the efficient quenching effect. The approach for the determination of serine shows a high sensitivity with a detection limit at 0.14 nM, which is lower than those of previous works. Furthermore, the present BNCDs system can be employed to monitor serine in real food and biological samples. The strategy may be a potential way for the application in food safety and biomedicine fields.

9.
Zhongguo Yi Liao Qi Xie Za Zhi ; 44(4): 346-352, 2020 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-32762212

RESUMO

This paper analyzes the causes and risks of common design and development changes of laser treatment equipment, studies the changes of key components and their corresponding control measures, forms the identification method of design changes of laser treatment equipment, and gives suggestions on how to deal with design and development changes, so as to provide references for inspectors during on-site inspection.


Assuntos
Terapia a Laser/instrumentação , Humanos , Fatores de Risco
10.
Cancer Lett ; 493: 120-127, 2020 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-32829006

RESUMO

LIM kinase 1 (LIMK1) and p21-activated kinase 4 (PAK4) are often over-expressed in breast tumors, which causes aggressive cancer phenotypes and unfavorable clinical outcomes. In addition to the well-defined role in regulating cell division, proliferation and invasion, the two kinases promote activation of the MAPK pathway and cause endocrine resistance through phosphorylating estrogen receptor alpha (ERα). PAK4 specifically phosphorylates LIMK1 and its functional partners, indicating possible value of suppressing both kinases in cancers that over-express PAK4 and/or LIMK1. Here, for the first time, we assessed the impact of combining LIMK1 inhibitor LIMKi 3 and PAK4 inhibitor PF-3758309 in preclinical breast cancer models. LIMK1 and PAK4 pharmacological inhibition synergistically reduced the survival of various cancer cell lines, exhibiting specific efficacy in luminal and HER2-enriched models, and suppressed development and ERα-driven signals in a BT474 xenograft model. In silico analysis demonstrated the cell lines with reliance on LIMK1 were the most prone to be susceptible to PAK4 inhibition. Double LIMK1 and PAK4 targeting therapy can be a successful therapeutic strategy for breast cancer, with a unique efficiency in the subtypes of luminal and HER2-enriched tumors.

11.
Artigo em Inglês | MEDLINE | ID: mdl-32776388

RESUMO

Interface strains and lattice distortion are inevitable issues during perovskite crystallization. Silane as a coupling agent is a popular connector to enhance the compatibility between inorganic and organic materials in semiconductor devices. Herein, a protonated amine silane coupling agent (PASCA-Br) interlayer between TiO2 and perovskite layers is adopted to directionally grasp both of them by forming the structural component of a lattice unit. The pillowy alkyl ammonium bromide terminals at the upper side of the interlayer provide well-matched growth sites for the perovskite, leading to mitigated interface strain and ensuing lattice distortion; meanwhile, its superior chemical compatibility presents an ideal effect on healing the under-coordinated Pb atoms and halogen vacancies of bare perovskite crystals. The PASCA-Br interlayer also serves as a mechanical buffer layer, inducing less cracked perovskite film when bending. The developed molecular-level flexible interlayer provides a promising interfacial engineering for perovskite solar cells and their flexible application.

12.
J Diabetes Investig ; 2020 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-32745370

RESUMO

AIMS/INTRODUCTION: The early pathological changes of diabetic peripheral neuropathy (DPN) are mainly small nerve fiber injuries. Corneal confocal microscopy (CCM) is an easy, rapid, non-invasive and repeatable technique to detect the damage of small nerve fibers. The purpose of this study was to explore the application of CCM in DPN and other chronic complications of type 2 diabetes mellitus. MATERIALS AND METHODS: A total of 220 individuals (48 normal healthy control participants and 172 patients with type 2 diabetes mellitus) were included in the study. All participants were assessed and scored for neurological symptoms and neurological deficits, quantitative sensory test, neuroelectrophysiological test, and CCM. RESULTS: Corneal nerve fiber density, corneal nerve fiber length and corneal nerve branch density were significantly reduced in patients with type 2 diabetes mellitus compared with normal healthy control subjects (P < 0.001, P < 0.001 and P < 0.001, respectively). In the DPN group, corneal nerve fiber density, corneal nerve branch density and corneal nerve fiber length were significantly lower than for patients without DPN (P < 0.001, P < 0.001 and P < 0.001, respectively). Receiver operating characteristic analysis showed that the optimal cut-off values were 24.68, 39 and 15.315, respectively, in which corneal nerve fiber density and corneal nerve fiber length had moderate sensitivity and specificity. CONCLUSION: This study provides more support for the clinical use of CCM to diagnose type 2 diabetes mellitus-related complications, especially DPN.

13.
Artigo em Inglês | MEDLINE | ID: mdl-32754448

RESUMO

Viral myocarditis (VMC) is a disease characterized as myocardial parenchyma or interstitium inflammation caused by virus infection, especially Coxsackievirus B3 (CVB3) infection, which has no accurate non-invasive examination for diagnosis and specific drugs for treatment. The mechanism of CVB3-induced VMC may be related to direct myocardial damage of virus infection and extensive damage of abnormal immune response after infection. Non-coding RNA (ncRNA) refers to RNA that is not translated into protein and plays a vital role in many biological processes. There is expanding evidence to reveal that ncRNAs regulate the occurrence and development of VMC, which may provide new treatment or diagnosis targets. In this review, we mainly demonstrate an overview of the potential role of ncRNAs in the pathogenesis, diagnosis and treatment of CVB3-induced VMC.

14.
Artigo em Inglês | MEDLINE | ID: mdl-32750882

RESUMO

Classification of electroencephalogram (EEG) signal data plays a vital role in epilepsy detection. Recently sparse representation-based classification (SRC) methods have achieved the good performance in EEG signal automatic detection, by which the EEG signals are sparsely represented using a few active coefficients in the dictionary and classified according to the reconstruction criteria. However, most of SRC learn a linear dictionary for encoding, and cannot extract enough information and nonlinear relationship of data for classification. To solve this problem, a hierarchical discriminative sparse representation classification model (called HD-SRC) for EEG signal detection is proposed. Based on the framework of neural network, HD-SRC learns the hierarchical nonlinear transformation and maps the signal data into the nonlinear transformed space. Through incorporating this idea into label consistent K singular value decomposition (LC-KSVD) at the top layer of neural network, HD-SRC seeks discriminative representation together with dictionary, while minimizing errors of classification, reconstruction and discriminative sparse-code for pattern classification. By learning the hierarchical feature mapping and discriminative dictionary simultaneously, more discriminative information of data can be exploited. In the experiment the proposed model is evaluated on the Bonn EEG database, and the results show it obtains satisfactory classification performance in multiple EEG signal detection tasks.

15.
Stem Cell Res ; 47: 101885, 2020 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-32610219

RESUMO

Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.

16.
Oncol Lett ; 20(2): 1906-1914, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32724434

RESUMO

Hypoxia, an important component of the tumor microenvironment, plays a crucial role in the occurrence and progression of cancer. However, to the best of our knowledge, a systematic analysis of a hypoxia-related prognostic signature for breast cancer is lacking and is urgently required. Therefore, in the present study, RNA-seq data and clinical information were downloaded from The Cancer Genome Atlas (TCGA) and served as a discovery cohort. Cox proportional hazards regression analysis was performed to construct a 14-gene prognostic signature (PFKL, P4HA2, GRHPR, SDC3, PPP1R15A, SIAH2, NDRG1, BTG1, TPD52, MAFF, ISG20, LALBA, ERRFI1 and VHL). The hypoxia-related signature successfully predicted survival outcomes of the discovery cohort (P<0.001 for the TCGA dataset). Three independent Gene Expression Omnibus databases (GSE10886, GSE20685 and GSE96058) were used as validation cohorts to verify the value of the predictive signature (P=0.007 for GSE10886, P=0.021 for GSE20685, P<0.001 for GSE96058). In the present study, a robust predictive signature was developed for patients with breast cancer, and the findings revealed that the 14-gene hypoxia-related signature could serve as a potential prognostic biomarker for breast cancer.

17.
Blood Adv ; 4(14): 3427-3434, 2020 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-32722780

RESUMO

Polyneuropathy, organomegaly, endocrinopathy, monoclonal plasma cell disorder, and skin changes (POEMS) syndrome is associated with increased risk for ischemic stroke (IS). Because POEMS syndrome is rare, little is known regarding the underlying mechanism and prognosis for IS in patients in whom it occurs. The medical records of patients with POEMS syndrome were screened between January 2018 and January 2000 at Peking Union Medical College Hospital to identify those with IS. The baseline characteristics, IS features, and patient outcomes were analyzed. Forty-one (8.0%) of 510 POEMS patients were documented to have IS. Patients with IS were older, had a higher percentage of Overall Neuropathy Limitation Scale score >4, and had a higher level of fibrinogen compared with those who did not have IS. Ninety-three percent of IS events occurred before or within 3 months after a diagnosis of POEMS. Of 41 occurrences of IS, 29 (46.3%) were multifocal. Recurrent IS was observed in 13 (31.7%) of 41 patients, but not in patients with successful anti-plasma cell therapy. The 3-year overall survival rate in patients with IS was 71.0% and for those without IS, it was 88.5% (P = .002). We showed that 8.0% of POEMS patients had IS, and most IS events occurred in POEMS patients not being treated effectively. Having IS was a predictor of unfavorable prognosis. Early diagnosis, immediate initiation of treatment for POEMS, and control of POEMS syndrome is key to reducing the occurrence of IS, improving survival, and preventing recurrence of IS.

18.
Microbiome ; 8(1): 112, 2020 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-32711581

RESUMO

BACKGROUND: The increasing incidence of cancer and intestinal mucositis induced by chemotherapeutics are causing worldwide concern. Many approaches such as fecal microbiota transplantation (FMT) have been used to minimize mucositis. However, it is still unknown whether FMT from a donor with beneficial gut microbiota results in more effective intestinal function in the recipient. Recently, we found that alginate oligosaccharides (AOS) benefit murine gut microbiota through increasing "beneficial" microbes to rescue busulfan induced mucositis. RESULTS: In the current investigation, FMT from AOS-dosed mice improved small intestine function over FMT from control mice through the recovery of gene expression and an increase in the levels of cell junction proteins. FMT from AOS-dosed mice showed superior benefits over FMT from control mice on recipient gut microbiotas through an increase in "beneficial" microbes such as Leuconostocaceae and recovery in blood metabolome. Furthermore, the correlation of gut microbiota and blood metabolites suggested that the "beneficial" microbe Lactobacillales helped with the recovery of blood metabolites, while the "harmful" microbe Mycoplasmatales did not. CONCLUSION: The data confirm our hypothesis that FMT from a donor with superior microbes leads to a more profound recovery of small intestinal function. We propose that gut microbiota from naturally produced AOS-treated donor may be used to prevent small intestinal mucositis induced by chemotherapeutics or other factors in recipients. Video Abstract.

19.
J Allergy Clin Immunol ; 146(3): 542, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32620310
20.
Artigo em Inglês | MEDLINE | ID: mdl-32628441

RESUMO

In this report, a solution-processable cohost system incorporating N,N'-di(naphtalene-1-yl)-N,N'-diphenylbenzidine (NPB) and Csp3-annulated phenylquinoline derivatives, including spiro[indeno[1,2-b]quinoline-11,8'-indolo[3,2,1-de]acridine] (IAIQ), 10-phenyl-10H-spiro[acridine-9,11'-indeno[1,2-b]quinoline] (PAIQ) and 3,3'-(11H-indeno[1,2-b]quinoline-11,11-diyl)bis(N-phenyl-N-(m-tolyl)aniline) (m-TPA-DPIQ), is developed for highly efficient saturated red phosphorescent organic light emitting diodes (OLEDs). IAIQ, PAIQ, and m-TPA-DPIQ, designed with the increase of molecular flexibility, are systematically investigated. Solution-processable devices based on the efficient phosphorescent emitter bis[2-(3,5-dimethylphenyl)isoquinolinato](2,8-dimethyl-4,6-nonanedionato)Iridium [Ir(mpiq)2divm] are successfully fabricated, and give electroluminescent peaks at 634-636 nm with Commission Internationale de L'Eclairage coordinates of (0.70, 0.30). Under optimized conditions, the devices incorporating IAIQ, PAIQ, and m-TPA-DPIQ exhibit high external quantum efficiency with the maximum value at 25.1%, 23.4%, and 23.3%, respectively, and all exceeding 18% at the luminance of 1000 cd/m2. In application, the supersaturated red devices with excellent performance could facilitate the development of wet-made displays. The newly developed Csp3-annulated host materials with their excitonic properties also showoff the tactic to construct cohost system for high-quality phosphorescent OLEDs.

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