RESUMO
The Mexican Hat wavelet (MHW) is strictly derived from the heat kernel by taking its negative first-order derivative with respect to time t. As a solution to the heat equation that the heat kernel has a clear initial condition, the Laplace-Beltrami operator. Although the MHW descriptor can effectively characterize the model information, but it has poor robustness to the model with scale transformation, and the feature description performance is affected to some extent. Following a popular mathematical method, in this paper, we bases on the MHW to study scaling invariance and proposes a new shape descriptor, the scale-invariant Mexican Hat wavelet (SIMHW), which by logarithmic sampling and Fourier transform that obtains the expression of SIMHW in Fourier domain. The experimental results show that SIMHW has finer information description ability and stronger recognition ability, and has better robustness to various non-rigid transformations. It can correctly calculate the similarity between 3D shapes and realize the effective shape retrieval.
Assuntos
Infecções por Coronavirus/prevenção & controle , Unidade Hospitalar de Ginecologia e Obstetrícia/organização & administração , Pneumonia Viral/prevenção & controle , Betacoronavirus , COVID-19 , Infecções por Coronavirus/epidemiologia , Feminino , Humanos , Pandemias , Pneumonia Viral/epidemiologia , Gravidez , SARS-CoV-2RESUMO
Abstract Long-chain non-encoded RNAs (lncRNAs) are important in many life activities and can participate in the occurrence of hepatocellular carcinoma (HCC). Moreover, lncRNAs can be used as basis for developing new strategies to hinder liver cancer. To investigate the utility of lncRNAs in HCC as potential biomarkers for early detection and diagnosis, we mined genomic data from the Cancer Genome Atlas (TCGA), and analyzed the gene expressions from 374 tumor patients and 50 normal patients. The abnormal expressions of 387 differentially expressed lncRNAs (DElncRNAs) were identified from a total of 3099 lncRNAs. Moreover, 18 modules were divided based on WGCNA, and 2 of the 18 modules were positively correlated with stage and grade, and negatively correlated with survival time. Finally, 10 lncRNAs were found and their main functions are the enhancement of cellular metabolic capacity and cell proliferation. These 10 lncRNAs may serve as novel prognostic markers and therapeutic targets, and may help guide subsequent studies on HCC.
Assuntos
Humanos , Biomarcadores Tumorais/genética , Marcadores Genéticos/genética , Carcinoma Hepatocelular/genética , RNA Longo não Codificante/genética , Neoplasias Hepáticas/genéticaRESUMO
An improved and efficient protocol was developed based on the TaKaRa RNAiso Plus Kit (Code: D9108A) for isolating good-quality total RNA from the optic stalk of mud crab, Scylla paramamosain. The protocol was based on the Trizol method with modifications. The carapace overlapping the optic stalk was retained with RNA in regular protocol. In order to remove the abundant deposition correlative with the carapace which makes the isolation of RNA particularly difficult, 5M potassium acetate solution (pH = 6.0) was added before the precipitation of RNA, and the temperature of RNA deposition was also decreased to -70ºC to ensure the stabilization of RNA. Good-quality total RNA from the optic stalk of S. paramamosain could be easily isolated with this modified protocol and three conventional methods were also employed to confirm the quality of RNA. This improved method would be helpful in facilitating molecular research of crabs involving RNA from the optic stalk.