Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.607
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
J Cell Physiol ; 235(1): 6-16, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31192453

RESUMO

MicroRNAs (miRNAs) are small noncoding RNAs about 19-22 nucleotides in length. Growing evidence has reported the significant role of miRNAs in various cancer-associated biological processes, such as proliferation, differentiation, apoptosis, metabolism, invasion, metastasis, and drug resistance. However, most studies focus on the targets of some individual miRNAs; the interactive and global functions of diverse miRNAs are still unclear and the phenomenon of the gathering of miRNAs in clusters has always been ignored. On the other hand, the fact that a single miRNA may regulate many genes and that numerous mRNAs are regulated by the same miRNA also makes it imperative to further study the cooperating characteristics of miRNAs in cancer. MiR-23a-27a-24-2 is located in the human chromosome 9q22, forming three mature miRNAs: miR-23a, miR27a, and miR-24, which are expressed abnormally in many malignant tumors. This review aims to summarize the interactive functions of miRNAs in miR-23a-27a-24-2 clusters in cancer from the perspectives of the regulation network, tumor microenvironment, and targeted therapy.

2.
J Cell Physiol ; 235(1): 394-407, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31225658

RESUMO

As the most commonly diagnosed malignant tumor in female population, the prognosis of breast cancer is affected by complex gene interaction networks. In this research weighted gene co-expression network analysis (WGCNA) would be utilized to build a gene co-expression network to identify potential biomarkers for prediction the prognosis of patients with breast cancer. We downloaded GSE25065 from Gene Expression Omnibus database as the test set. GSE25055 and GSE42568 were utilized to validate findings in the research. Seven modules were established in the GSE25065 by utilizing average link hierarchical clustering. Three hub genes, RSAD2, HERC5, and CCL8 were screened out from the significant module (R 2 = 0.44), which were considerably interrelated to worse prognosis. Within test dataset GSE25065, RSAD2, and CCL8 were correlated with tumor stage, grade, and lymph node metastases, whereas HERC5 was correlated with lymph node metastases and tumor grade. In the validation dataset GSE25055 and RSAD2 expression was correlated with tumor grade, stage, and size, whereas HERC5 was related to tumor stage and tumor grade, and CCL8 was associated with tumor size and tumor grade. Multivariable survival analysis demonstrated that RSAD2, HERC5, and CCL8 were independent risk factors. In conclusion, the WGCNA analysis conducted in this study screened out novel prognostic biomarkers of breast cancer. Meanwhile, further in vivo and in vitro studies are required to make the clear molecular mechanisms.

3.
Cytokine ; 125: 154850, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31557635

RESUMO

BACKGROUND: Endometrial injury can result in thin endometrium and subfertility. Granulocyte macrophage colony stimulating factor (GM-CSF) contributes to tissue repair, but its role in endometrial regeneration has not been investigated. METHODS: To determine the effect of GM-CSF on endometrial regeneration, we established a mouse model of thin endometrium by uterine perfusion with 20 µL 90% ethanol. Thin endometrium in mice was featured by lowered endometrial thickness, decreased expression of Ki67 in glandular cells, and a reduced number of implantation sites. To explore the mechanism of GM-CSF on endometrial regeneration, endometrium was obtained from patients undergoing hysterectomy or hysteroscopy and endometrial biopsy. Effects of GM-CSF on primary cultured human endometrial glandular and stromal cells were examined by the 5-bromo-2'-deoxyuridine (BrdU) proliferation assay and transwell migration assay, followed by exploration of the potential signaling pathway. RESULTS: GM-CSF intraperitoneal (i.p.) injection significantly increased endometrial thickness, expression of Ki67 in endometrial glandular cells, and the number of implantation sites. GM-CSF significantly promoted proliferation of primary human endometrial glandular cells and migration of stromal cells. GM-CSF activated p-Akt and increased expressions of p70S6K and c-Jun, which were blocked by LY294002. CONCLUSION: We found that GM-CSF could improve endometrial regeneration, possibly through activating PI3K/Akt signaling pathway.

4.
Bioresour Technol ; 295: 122298, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31675521

RESUMO

Four lab-scale moving bed biofilm reactors (MBBRs) were built to treat simulated wastewater containing typical pharmaceuticals and personal care products (PPCPs). The efficiency in removing different PPCPs at different concentrations (1, 2 and 5 mg/L) and their effects on the performance of MBBRs were investigated. Results showed that the average removal efficiencies of sulfadiazine, ibuprofen and carbamazepine were 61.1 ±â€¯8.8%, 74.9 ±â€¯8.8% and 28.3 ±â€¯7.4%, respectively. Compared to the reactor without PPCPs, the total nitrogen (TN) removal efficiency of the reactors containing sulfadiazine, ibuprofen and carbamazepine declined by 21%, 30% and 42%, respectively. Based on the microbial community analysis, increasing the PPCPs concentration within a certain range (<2 mg/L) could stimulate microbial growth and increase microbial diversity yet the diversity reduced when the concentration (5 mg/L) exceeded the tolerance of microorganisms. Furthermore the presence and degradation of different PPCPs resulted in a different kind of microbial community structure in the MBBRs.


Assuntos
Reatores Biológicos , Microbiota , Biofilmes , Nitrogênio , Eliminação de Resíduos Líquidos , Águas Residuárias
5.
Mol Cell Endocrinol ; 499: 110589, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31557499

RESUMO

Polycystic ovary syndrome (PCOS) is an endocrine disorder, the etiology of which is complex and unclear. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a conserved long non-coding RNA which has been found to play a role in the pathophysiological process of reproductive system diseases, such as endometriosis and pregnancy loss. However, the role of MALAT1 in PCOS is still unknown. In this study, reduced MALAT1 expression was found in granulosa cells (GCs) from 68 patients with PCOS and 30 healthy controls, which relates to upregulated cell proliferation and downregulated apoptosis. Using phosphorylation pathway profiling array, MALAT1 reduction was identified to contribute to the repression of transforming growth factor beta (TGFß) signaling in GCs. Subsequently, MALAT1 was confirmed to function as a competing endogenous RNA (ceRNA), interacting with miR-125b and miR-203a. Meanwhile, miR-125b and miR-203a was identified as two novel TGFß signaling negative regulators by targeting TGFBR1 and TGFBR2. Finally, MALAT1 knockdown was found to induce the upregulation of miR-125b and miR-203a, which further repressed TGFß signaling, changed some downstream gene expression, and resulted in a disordered cell cycle. In conclusion, MALAT1 reduction was identified in GCs, which may contribute to the pathophysiological processes of PCOS by regulating TGFß signaling through sponging miR-125b and miR-203a.

6.
Fitoterapia ; 139: 104407, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31705949

RESUMO

Chemical investigations of the aerial parts of Tibetan folk medicine Delphinium chrysotrichum resulted in the isolation of two new diterpenoid alkaloids, delphatisine D (1) and chrysotrichumine A (2), together with ten known diterpenoid alkaloids (3-12) and 3ß,6α-dihydroxysclareolida (13). Delphatisine D (1) is a rare atisine-type alkaloid from genus Delphinium and is the C-15 epimer of spiramine C which bears an internal carbinolamine ether linkage (N-C-O-C) between C-7 and C-20. Chrysotrichumine A (2) is a rare natural C19-diterpenoid alkaloid possessing a nitrone group between C-17 and C-19. Their structures were elucidated on the basis of extensive spectroscopic analysis. Compound 13 is a sesquiterpene first isolated from this genus. Compounds 1, 3-7 and 13 were investigated for cytotoxicity against human breast cancer cell lines of MCF-7 and MDA-MB-23, none of them presented significant activity except compound 7 which exhibited slight activity at 100 µM against MDA-MB-231, but did not reach the EC50.

7.
Org Biomol Chem ; 17(46): 9844-9848, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31724684

RESUMO

A rhodium-catalyzed three-component reaction of diazo compounds, anilines and C,N-cyclic azomethine imines via trapping of transient ammonium ylides was developed. This reaction provided a simple and convenient approach for the synthesis of pharmaceutically intriguing tetrahydroisoquinoline derivatives in moderate to good yields (36-85%) with good diastereoselectivities (up to 95 : 5 dr) under mild reaction conditions.

8.
Adv Exp Med Biol ; 1101: 41-65, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31729671

RESUMO

Brain-computer interfaces (BCIs) provide a direct communication channel between human brain and output devices. Due to advantages such as non-invasiveness, ease of use, and low cost, electroencephalography (EEG) is the most popular method for current BCIs. This chapter gives an overview of the current EEG-based BCIs for the main purpose of communication and control. This chapter first provides a taxonomy of the EEG-based BCI systems by categorizing them into three major groups: (1) BCIs based on event-related potentials (ERPs), (2) BCIs based on sensorimotor rhythms, and (3) hybrid BCIs. Next, this chapter describes challenges and potential solutions in developing practical BCI systems toward high communication speed, convenient system use, and low user variation. Then this chapter briefly reviews both medical and non-medical applications of current BCIs. Finally, this chapter concludes with a summary of current stage and future perspectives of the EEG-based BCI technology.


Assuntos
Interfaces Cérebro-Computador , Eletroencefalografia , Comunicação , Potenciais Evocados , Humanos
9.
Brain Res Bull ; 154: 32-42, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31669104

RESUMO

Glial cell line-derived neurotrophic factor (GDNF) has neurotrophic activity for the survival of dopaminergic neurons, which is under active investigation for Parkinson's disease (PD) therapy. FLZ is a potential new drug for PD treatment. However, it is unclear whether neurotrophic activity contributes to the neuroprotective effects of FLZ. Here we found that FLZ markedly improved the function of dopaminergic neurons in primary mesencephalic neuron/glia cultures. Further investigation demonstrated that astroglia were required for FLZ to function as a neurotrophic regulator, as FLZ failed to show neurotrophic effects in the absence of astroglia. We clarified that GDNF was responsible for the neurotrophic effects of FLZ since FLZ selectively stimulated GDNF production, which was confirmed by the finding that the neurotrophic effect of FLZ was attenuated by GDNF-neutralizing antibody. Mechanistic study demonstrated that GDNF induction by FLZ was CREB-dependent and that PI3K/Akt was the main pathway regulating CREB activity, which was confirmed by in vivo studies. We also validated that the induction of GDNF by FLZ contributed to PD treatment in vivo. In conclusion, the present data provided evidence that FLZ had robust neurotrophic effects on dopaminergic neurons through sustained induction of GDNF in astroglia by activating the PI3K/Akt/CREB pathway.

10.
Neurobiol Dis ; 134: 104630, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31678404

RESUMO

Multiple sclerosis (MS) is a chronic autoimmune demyelinating disease characterized by the autoimmune attack of oligodendrocytes, leading to demyelination and progressive functional deficits. CXC chemokine receptor 2 (CXCR2) is recently reported to orchestrate the migration, proliferation and differentiation of oligodendrocyte precursor cells (OPCs), which implies its possible involvement in the demyelinating process. Here, we used a CXCR2 antagonist, compound 2, as a tool to investigate the role of CXCR2 in demyelination and the underlying mechanism. The primary cultured oligodendrocytes and cuprizone (CPZ)-intoxicated mice were applied in the present study. The results showed that compound 2 significantly promoted OPC proliferation and differentiation. In the demyelinated lesions of CPZ-intoxicated mice, vigorous OPC proliferation and myelin repair was observed after compound 2 treatment. Subsequent investigation of the underlying mechanisms identified that upon inhibition of CXCR2, compound 2 treatment upregulated Ki67, transcription factor 2 (Olig2) and Caspr expression, activated PI3K/AKT/mTOR signaling, ultimately promoted OPCs differentiation and enhanced remyelination. In conclusion, our results demonstrated that CXCR2 antagonism efficiently promoted OPC differentiation and enhanced remyelination in CPZ-intoxicated mice, supporting CXCR2 as a promising therapeutic target for the treatment of chronic demyelinating diseases such as MS.

11.
J Org Chem ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31702914

RESUMO

A zinc-catalyzed intermolecular alkyne-carbonyl metathesis reaction of ynamides with isatins followed by an amide to ester conversion has been developed, which produces the indolone derivatives with a fully substituted alkene species in good to high yields. The salient features of this reaction include the following: mild reaction conditions, an inexpensive zinc catalyst, a broad substrate scope, the excellent regiocontrol and stereoselectivity, and amenable to the gram scale.

12.
J Ovarian Res ; 12(1): 109, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722718

RESUMO

Polycystic ovary syndrome (PCOS) is a complex endocrine disorder and regarded as the leading cause of anovulatory infertility. PCOS is characterized by reproductive dysfunction and metabolic disorders. Baicalin (BAL) is one of the most potent bioactive flavonoids isolated from the radix of Scutellaria baicalensis. In the present study, we investigated the potential effects of BAL on PCOS in dehydroepiandrosterone-treated rats. We found that BAL notably reduced the serum levels of free testosterone, total testosterone, follicle-stimulating hormone, luteinizing hormone, progesterone, and estradiol in PCOS rats. The increase of serum insulin level and HOMA-IR was markedly inhibited by BAL. Moreover, BAL decreased body weights, increased the number of rats with the regular estrous cycle, and ameliorated ovarian histological changes and follicular development in the DHEA-treated PCOS rats. The increase of pro-inflammatory cytokines (TNFα, IL-1ß, and IL-18) and decrease of anti-inflammatory cytokine (IL-10) in PCOS rats were suppressed by BAL. BAL induced a significant decrease in the mRNA expression of steroidogenic enzymes, including 3ß-HSD, CYP11A1, CYP19A1, StAR, in ovarian tissues in PCOS rats. Furthermore, BAL inhibited the decrease of AMPK protein level and phosphorylation, the decrease of Akt phosphorylation and the increase of 5α-reductase enzyme 1 expression in ovarian tissues in PCOS rats. The effects of BAL were inhibited by an inhibitor of AMPK, dorsomorphin. The upregulation of AMPK contributed to the beneficial effects of BAL. The results highlight the potential role of BAL for the intervention of PCOS.

13.
Biosci Rep ; 39(11)2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31702007

RESUMO

OBJECTIVES: The present study aimed at investigating the therapeutic effect of Salidroside on skeletal muscle atrophy in a rat model of cigarette smoking-induced chronic obstructive pulmonary disease (COPD) and its potential mechanisms. METHODS: Male Wistar rats were randomized, and treated intraperitoneally (IP) with vehicle (injectable water) or a low, medium or high dose of Salidroside, followed by exposure to cigarette smoking daily for 16 weeks. A healthy control received vehicle injection and air exposure. Their lung function, body weights and gastrocnemius (GN) weights, grip strength and cross-section area (CSA) of individual muscular fibers in the GN were measured. The levels of TNF-α, IL-6, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) in serum and GN tissues as well as myostatin and myogenin expression in GN tissues were measured. RESULTS: In comparison with that in the healthy control, long-term cigarette smoking induced emphysema, significantly impaired lung function, reduced body and GN weights and CSA values in rats, accompanied by significantly increased levels of TNF-α, IL-6 and MDA, but decreased levels of SOD and GSH in serum and GN tissues. Furthermore, cigarette smoking significantly up-regulated myostatin expression, but down-regulated myogenin expression in GN tissues. Salidroside treatment decreased emphysema, significantly ameliorated lung function, increased antioxidant, but reduced MDA, IL-6 and TNF-α levels in serum and GN tissues of rats, accompanied by decreased myostain, but increased myogenin expression in GN tissues. CONCLUSION: Salidroside mitigates the long-term cigarette smoking-induced emphysema and skeletal muscle atrophy in rats by inhibiting oxidative stress and inflammatory responses and regulating muscle-specific transcription factor expression.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31755059

RESUMO

To reveal the adsorption mechanism of sediment to antibiotics with the presence of dissolved organic matter (DOM), batch experiments were carried out by oxytetracycline (OTC) on sediments with decayed plants (PDOM) and composted chicken manure (MDOM), and the zeta potential in the system before and after adsorption was measured. Results showed that the PDOM promoted the adsorption process, while the MDOM inhibited the adsorption. Adding PDOM, the change of zeta potential (Δζ) increased by 40.08% for first terrace sediments (FT) and 63.98% for riverbed sediments (RB), respectively; meanwhile, MDOM decreased by 20.04% for FT and 28.39% for RB, respectively. The results of kinetic fitting models of replacing the adsorption amount with Δζ were consistent with the initial. It indicated that there was a positive correlation between the adsorption amount and Δζ, and the zeta potential can be used to quickly judge the degree of adsorption process. The Derjaguin-Landau-Verwey-Overbeek (DLVO) theory describes the interactions of sediment particles. In terms of adsorption amount, zeta potential (absolute value) and total interaction energy all followed the order: RB > FT, RB-PDOM > FT-PDOM, and RB-MDOM > FT-MDOM. The more negative the zeta potential is, the better the dispersion of the particles is. Stronger repulsion is more conducive to adsorbing positively charged OTC. The site energy distribution theory further explained that the distribution of adsorption site in the various states of sediments increased while adding the PDOM and decreased while adding the MDOM.

15.
Sci China Life Sci ; 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31745693

RESUMO

Rapid weight gain (RWG) in infants is associated with numerous health problems, and its risk factors are still unclear. We assessed 98,097 maternal-infant pairs from a population-based cohort study and followed up with them until the infants were 6 months old. We assessed the associations between maternal prepregnancy weight status; gestational weight gain; feeding pattern; and infants' RWG at 0-1, 0-3, 1-3, and 3-6 months using multivariate unconditional logistic regression models, with controlled confounders. We found that maternal prepregnancy weight status, gestational weight gain, and feeding pattern at the 1st, 3rd, and 6th months had significant impacts on the infants' RWG at each time period (P<0.05). Infants with overweight/obese mothers had a higher risk of RWG after birth, whereas those of mothers who experienced excessive gestational weight gain had higher risks of RWG from birth than the other groups (P<0.01). Infants who were formula-fed had a higher risk of RWG than breastfed infants at the same time point (P<0.01). In conclusion, maternal prepregnancy obesity, excessive gestational weight gain, and formula-feeding were risk factors for infants' RWG during the first 6 months of life.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31711227

RESUMO

OBJECTIVE: To explore whether quality control circle (QCC) is associated with hospital staff's perceptions of patient safety culture (PSC). DESIGN: A cross-sectional survey in 12 public hospitals from October to December 2018 and a longitudinal survey in one public hospital from November 2017 to November 2018. SETTING: In 12 public hospitals from six provinces located in eastern, central and western of China, and one public hospital in eastern China. PARTICIPANTS: In total, 811 and 102 hospital staff participated in the cross-sectional survey and the longitudinal survey, respectively. These participants included doctors, nurses, medical technicians and administrative staff. MAIN OUTCOME MEASURES: Hospital staff's perceptions of PSC were measured by the Hospital Survey on Patient Safety Culture (HSOPSC) questionnaire. The association between QCC implementation and PSC was identified by univariate analysis and multiple linear regression analysis. RESULTS: Univariate analysis showed that the staff from hospitals that had implemented QCC received significantly higher HSOPSC scores than those from hospitals where QCC had not been implemented (3.73 ± 0.61 vs. 3.57 ± 0.41, P < 0.05). The QCC implementation was a significant predictor in the established multiple linear regression model. One year after QCC implementation, the hospital involved in the longitudinal survey scored higher in HSOPSC than before (3.75 ± 0.42 vs. 3.60 ± 0.36, P < 0.001). CONCLUSIONS: QCC implementation was positively associated with PSC and the former could promote the establishment of the latter. It is suggested that QCC can play an active role in enhancing PSC so as to further improve patient safety management.

18.
Curr Pharm Des ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31696804

RESUMO

Bioluminescent proteins (BLPs) are widely distributed in many living organisms that act as a key role of light emission in the bioluminescence. Bioluminescence serves various functions in finding food and protecting themselves of lives of creatures. With the routinely biotechnological application of bioluminescence, it is recognized to be essential for many medical, commercial and other general technological advances. Therefore, the prediction and characterization of BLPs is significant and eager in that it could help to explore more secrets about bioluminescence and promote the development of application of bioluminescence. Since the experimental methods are money and time-consuming for BLPs identification, bioinformatics tools have played important parts for fast and accurately predicting BLPs by combining their sequences information with machine learning methods. In this review, we summarized and compared the application of machine learning methods in the prediction of BLPs from different aspects. We wish that this review will provide insights and inspirations for the researches on BLPs.

19.
Anal Chem ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31714062

RESUMO

With the ever-increasing demand for improved medical diagnosis, safe food supply, advanced biotechnology, and sustainable ecosystems, characterization of the microbial world by sensitive, specific, rapid, and quantitative tools is gaining more and more attentions. Surface-enhanced Raman spectroscopy (SERS) and its flexible integration with other tools, such as microscopes, stable isotope probing, microfluidic analysis, and chemometric analysis, have advanced rapidly and showed great promise for versatile microbial characterization. This perspective provides an overview of the recent advances of SERS and related coupling techniques for microbial diagnosis (e.g., identification and antibiotic resistance testing), phenotypic response profiling, microbial function assessment, in situ biofilm characterization, and multifunctional SERS tags. We further propose future requirements and the direction for SERS methodology to be headed, including standardization of SERS methodology, database generation and management, coupling with omics technology and single-cell sorting, and application in deciphering microbial processes.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA