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1.
Talanta ; 206: 120204, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514833

RESUMO

As a rapid and facile means for foodborne bacteria detection in situ, lateral flow immunoassay (LFA) still has intrinsic limitations in the construction of the existing sandwich LFA format, e.g. screening difficulties of paired antibodies (Abs), poor stability of Ab probe, etc. Here, combined the strong affinity of antibiotic with the superior specificity of antibody molecules, a novel and robust LFA based on a dual recognition strategy and magnetic separation was designed to achieve specific and sensitive determination of Salmonella enteritidis (S. enteritidis). In this work, ampicillin (Amp), a broad-spectrum antibiotic against bacteria, was employed as an ideal Ab replacer to anchor cells of target bacteria. By coating Amp on magnetite nanoparticles (MNPs), the Amp-MNPs showed remarkable binding, separation and enrichment capacities toward bacteria even under complex sample matrices. To ensure the selectivity of this protocol, anti-S. enteritidis monoclonal antibody was then adopted as the second anchoring agent to form a sandwich complex with Amp-MNPs. Based on these facts, S. enteritidis, as low as 102-103 CFU/mL, could be detected by naked eyes in food samples. Therefore, this creative antibiotic-bacteria-antibody LFA sandwich pattern shows great application potential in the monitoring of food contamination and infectious diseases caused by pathogenic bacteria. Compared to the common paired Abs based sandwich method, the proposed approach was cost-effective, non-labor intensive, stable, sensitive and efficient.

2.
Environ Technol ; : 1-6, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31630640

RESUMO

Sample pretreatment process is usually performed before real sample detection. Extraction is one of the most prevalent pretreatment methods. Due to the unequal solubility in two liquid phases, target is extracted (usually from water to organic phase) and further detected with laboratory instruments. Although the analytical instruments have high sensitivity and selectivity, they are relatively expensive and require skilful hands, which is restricted to laboratory use. Here, paper-based analytical devices (µPADs) for measuring samples extracted with organic solvents were tested. The µPADs were employed as a filter for buffer exchange, allowing the transfer of targets from organic solvent to water-based buffer, which could be specifically detected by water-based assays. As an example, polychlorinated biphenyls (PCBs) in n-hexane were detected with aptamer and gold nanoparticles (AuNPs) on µPADs within 30 min. The result of colour change can be recognized from 70.0 ng/L with a low loading amount (0.5 µL). Instead of laboratory instrumental analysis with relative high cost, this assay showed the promising utility of µPADs for target detection after sample pretreatment, which could be utilized as a general platform for inexpensive onsite detection.

3.
ACS Appl Mater Interfaces ; 11(39): 36278-36285, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31490648

RESUMO

Carbon nanotube (CNT) buckypapers, or films, have the potential for wide applications because of their unique properties. Neat buckypapers or pristine CNT (PCNT) films have relatively large elongation but low strength and low modulus due to the weak interaction between CNTs. Chemical modifications of PCNT films can significantly strengthen the interaction between CNTs, resulting in high strength and high modulus but usually accompanied by low elongation. Here, we report the functionalization of pristine CNT films by thiol-ended hyperbranched polymers (THBP-n) via a thiol-ene click reaction that can introduce simultaneous improvements on the strength, modulus, and elongation to the PCNT film by 689, 812, and 32.4%, respectively. The high thiol content of THBP-n enables the formation of a network with a high degree of cross-linking between carbon nanotubes, which provides high-efficiency load transfer that increases the tensile strength and modulus of the resulting films and at the same time a compressible hyperbranched structure that allows for deformation and slip between CNTs and consequently improved elongation. The main factors affecting the mechanical performance of the functionalized CNT film are also investigated.

4.
J Agric Food Chem ; 67(23): 6642-6649, 2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-31117488

RESUMO

The rapid monitoring of foodborne pathogens by monoclonal antibody (McAb)-based immunochromatographic tests (ICTs) is desirable but highly challenging as a result of the screening obstacle for a superior performance probe, which will greatly determine the capture efficiency of targets and the sensitivity of the immunoassay. In this work, on the basis of two-dimensional (2D) nanosheets (including MoS2 and graphene) as the extraordinary capture probe and signal indicator, we fabricated a label-free ICT method for Salmonella enteritidis detection. Especially, without the customarily labeled antibody probe, these 2D versatile probes presented strong capture ability toward bacteria by directly assembling onto the surface of bacteria. An ideal analytical performance with high sensitivity and specificity was achieved by virtue of the novel nanosheet-bacteria-McAb sandwich format. On the basis of MoS2 2D nanosheets as a fabulous probe element, the developed ICT exhibited a lowest detectable concentration of 103 colony-forming units/mL for S. enteritidis and could be well-applied in drinking water and watermelon juice samples. By the smart design, this work removes a series of conditionality issues of traditional double antibody sandwich-based ICTs and can give a new application direction for 2D nanosheet materials in the rapid detection field.


Assuntos
Água Potável/microbiologia , Sucos de Frutas e Vegetais/microbiologia , Imunoensaio/métodos , Salmonella enteritidis/isolamento & purificação , Citrullus/química , Citrullus/microbiologia , Grafite/química , Imunoensaio/instrumentação , Nanoestruturas/química , Salmonella enteritidis/química , Sensibilidade e Especificidade
5.
Nanoscale ; 11(15): 7188-7198, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30919872

RESUMO

Electrochemical conversion reactions offer a new avenue to build high-capacity anodes for sodium-ion batteries. However, poor long-term cyclability and low coulombic efficiency at the first cycle remain a significant challenge for practical Na-ion battery applications. Herein, a novel hyperbranched polymer is used as a template and electrode additive to construct unique hierarchical Cu9S5 nanoplates. With an internal uniform distribution, the additive could regulate the morphology and microstructure of Cu9S5 and offer a buffering matrix to alleviate nanoparticle aggregation and enhance solid-state Na+ ion diffusion. This Cu9S5 composite anode exhibits a high reversible capacity of 429 mA h g-1 at 100 mA g-1, a high coulombic efficiency of 94.3% at the first cycle, a superior rate capability of 300 mA h g-1 at 20 A g-1, and an outstanding cyclability with 82.2% capacity retention after 1000 cycles. The kinetic study reveals that Cu9S5-AHP nanoplates show a low charge transfer resistance and high Na+ diffusion coefficient (∼10-9 cm2 s-1). The present work suggests a potentially feasible anode material for sodium-ion batteries and, more significantly, demonstrates a novel strategy for the construction of high-performance conversion materials for sodium-ion batteries.

6.
Biosens Bioelectron ; 132: 360-367, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30897543

RESUMO

Lateral flow immunoassay (LFIA) is a class and widespread applied point-of-care biosensor in the rapid monitoring field. To address the matched antibodies and antibody labeling dependence in the conventional LFIAs, in this work, an innovative label-free LFIA was proposed for the sensitive detection of Salmonella enteritidis (S. enteritidis) by introducing a new nanoparticles-bacteria-antibody sandwich strategy in the sensor. Surface positively charged nitrogen-rich carbon (pNC) nanoparticles, synthesized via calcination and etching reactions, were used as adsorbent to capture bacteria as well as for generating signals. In the presence of target pathogens, bacterial cells could combine with pNC through electrostatic interaction and hydrogen bonding, then the complex would be captured specifically by the anti-bacteria monoclonal antibody (McAb) coated on the test line (T-line). With the accumulation of nanoparticles-bacteria, the color on T-line would be gradually deepened from nearly colorless to deep black. Importantly, the pNC-based immunoassay could exhibit high sensitivity for target pathogens detection with a linear range of 102-108 cfu mL-1 and a low detection limit of 102 cfu mL-1. Furthermore, this system was validated preliminarily to screen S. enteritidis in different food samples with recoveries ranging from 85% to 110%. Taking advantages of simplicity, label-free, convenience, and sensitivity, the pNC-based LFIA has the application potential for pathogenic microorganisms monitoring in food safety and early clinical diagnosis fields.


Assuntos
Anticorpos Imobilizados/química , Técnicas Biossensoriais/instrumentação , Carbono/química , Análise de Alimentos/instrumentação , Nanopartículas/química , Nitrogênio/química , Salmonella enteritidis/isolamento & purificação , Microbiologia de Alimentos , Humanos , Imunoensaio/instrumentação , Limite de Detecção , Modelos Moleculares , Fitas Reagentes/análise , Infecções por Salmonella/microbiologia
7.
Food Chem ; 274: 816-821, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30373015

RESUMO

Here, a facile, label-free and sensitive lateral flow strip (LFS) biosensor for foodborne pathogens was established relying on the innovative introduction of Gram staining and the direct immunoreaction. Target bacteria can be directly marked with crystal violet (CV) by one-step staining which is superior to traditional signal marking techniques in LFS assay, and the method's selectivity can be guaranteed by high-specificity monoclonal antibody. With Salmonella Enteritidis (S. Enteritidis) as a model target, this protocol can selectively detect 80 CFU mL-1S. Enteritidis within 11 min in the optimized conditions. Moreover, with Listeria monocytogenes as another model target, the biosensor shows a high universality for detections of both gram-negative and gram-positive bacteria. The unexpected applicability of biological dye tracer in strip biosensor reveals that the biological dye can be a potential tool serving as a universal signal tracer for pathogenic microorganisms in food safety monitoring and early clinical diagnosis.


Assuntos
Técnicas Biossensoriais/métodos , Microbiologia de Alimentos/métodos , Listeria monocytogenes/imunologia , Salmonella enteritidis/imunologia , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Água Potável/microbiologia , Inocuidade dos Alimentos/métodos , Violeta Genciana , Limite de Detecção , Lycopersicon esculentum/microbiologia , Fenazinas , Carne Vermelha/microbiologia
8.
Dalton Trans ; 47(47): 17146-17152, 2018 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-30467563

RESUMO

One-dimensional supercapacitors (SCs) are of great interest for future wearable electronics, but improvement in both high capacitance and high flexibility is still a challenge. Herein, we fabricate a high performance yarn asymmetric SC (ASC) using in situ prepared CuCo2O4 nanowires and polypyrrole (PPy) nanoparticles on the surface of a two-ply carbon nanotube (CNT) yarn. The parallel-shaped yarn ASC not only shows outstanding redox pseudocapacitance, including a high areal capacitance (59.55 mF cm-2), specific energy density (20 µW h cm-2) and power density (5.115 mW cm-2), but also exhibits good flexibility because of its well maintained capacitance during repeated dynamic bending states. The capacitance retention still remains 80.1% under 8000 cycles, suggesting relatively good reversibility and stability.

9.
Nanoscale ; 10(47): 22329-22334, 2018 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-30468232

RESUMO

Conventional flexible supercapacitors can work under consecutive bending, folding and even twisting without performance degradation. Nevertheless, these devices can hardly be used under large tensile strain. Flexible stretchable and healable supercapacitors are highly desired due to their many potential applications in electric devices. However, it is challenging to fabricate supercapacitors that can withstand stretchability and self-healability. Herein, we report a stretchable and self-healable supercapacitor based on a carbon nanotube@graphene@PANI nanowire film. The supercapacitor possesses high energy density from 36.3 to 29.4 µW h cm-2 with the corresponding power density changing from 0.17 to 5 mW cm-2 at a current from 0.1 to 3 mA, and the highest capacitive performance can reach up to 261.5 mF cm-2. In terms of the bending test, the supercapacitor can operate under different static bending angles and dynamic bending conditions with different bending frequencies, and the capacitance was merely affected. Moreover, the supercapacitor can sustain a tensile strain up to 180% and 80.2% capacitance retention after the 10th healing cycle. This novel design integrating all stretchable and healable components provides a pathway toward the next generation of wearable energy devices in modern electronics.

10.
Chemistry ; 24(45): 11667-11674, 2018 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-29851158

RESUMO

The recognized therapeutic benefits from carbon monoxide (CO) have caused booming attention to develop a CO therapy for various major diseases, such as cancer. However, the controlled release of CO gas and the monitoring of the CO release are vitally important to the on-demand CO administration for a safe and efficient therapy, but greatly challenging. In this work, a new CO-releasing nanocomplex was constructed by the adsorption and coordination of manganese carbonyl ([MnBr(CO)5 ], abbreviated as MnCO) with a Ti-based metal-organic framework (Ti-MOF) to realize an intratumoral H2 O2 -triggered CO release and real-time CO release monitoring by fluorescence imaging. A high CO prodrug loading capacity (0.532 g MnCO per gram Ti-MOF) is achieved due to the high surface area of Ti-MOF, and the intracellular H2 O2 -triggered CO release from the MnCO@Ti-MOF is realized to enable the nanocomplex selectively release CO in tumor cells and kill tumor cells rather than normal cells. Particularly significant is that the real-time fluorescence imaging monitoring of the CO release is realized based on an annihilation effect of the fluorescence after MnCO loading into Ti-MOF and an activation effect of the fluorescence after CO release from Ti-MOF. The quantitative relationship between the fluorescence intensity and the released CO amount is established in great favor of guiding on-demand CO administration. The results demonstrate the advantage of versatile MOFs for high efficient CO delivery and monitoring, which is critical for the improvement of the effectiveness of future therapeutic application.

11.
Dalton Trans ; 47(23): 7747-7753, 2018 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-29808194

RESUMO

In this work, hollow RuO2 nanotube arrays were successfully grown on carbon cloth by using a facile two-step method to fabricate a binder-free electrode. The well-aligned electrode displays excellent electrochemical performance. By using RuO2 hollow nanotube arrays as the positive electrode and Fe2O3 as the negative electrode, a flexible solid-state asymmetric supercapacitor (ASC) has been fabricated which exhibited excellent electrochemical performance, such as a high capacitance of 4.9 F cm-3, a high energy density of 1.5 mW h cm-3 and a high power density of 9.1 mW cm-3. In addition, the two-electrode SC shows high cycling stability with 97% capacitance retention after 5000 charge-discharge cycles. These excellent electrochemical performances are ascribed to the unique hollow structural design of electrodes, which can shorten the ion diffusion length, provide a fast ion transport channel, and offer a large electrode/electrolyte interface for the charge-transfer reaction. The structural design and the synthesis approach are general and can be extended to synthesizing a broad range of materials systems.

12.
Food Chem ; 261: 131-138, 2018 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-29739573

RESUMO

We presented a signal amplified lateral flow assay (LFA) based on magnetite nanoparticles (MNPs) labeled dual-probe and applied it in the high sensitive and rapid on-site detection of furazolidone metabolite of 3-amino-2-oxazolidinone (AOZ). The amplified signal benefited from high affinity between two probes of MNPs labeled murine monoclonal antibody (MNPs-MAb) and goat anti-mouse antibody (MNPs-GAMA) and was achieved by the generation of dual-probe network complex. This developed method could realize high sensitive detection of AOZ with a threshold value of 0.88 ng mL-1 and a detection limit of 0.044 ng mL-1, the sensitivity was at least 10-fold improved than that of the traditional gold nanoparticle based LFA. This facile developed assay was successfully applied for rapid detection of AOZ in milk samples. The proposed method paves a new way for on-site screening of other hazardous substances in food and can be referred in all lateral flow assays.


Assuntos
Análise de Alimentos/métodos , Furazolidona/análise , Nanopartículas de Magnetita/química , Leite/química , Animais , Furazolidona/química , Limite de Detecção , Oxazolidinonas/análise
13.
Food Chem ; 262: 48-55, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29751920

RESUMO

Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.


Assuntos
Técnicas Biossensoriais/métodos , Cromatografia de Afinidade/métodos , Clembuterol/análise , Escherichia coli , Nanopartículas Metálicas/química , Animais , Anticorpos/imunologia , Antígenos , Cromatografia de Afinidade/instrumentação , Feminino , Ouro/química , Limite de Detecção , Camundongos Endogâmicos BALB C , Sensibilidade e Especificidade
14.
Sci Total Environ ; 631-632: 912-920, 2018 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-29728002

RESUMO

Lead(II) is a highly toxic upon accumulation in the human body and a thorough cleanup of this pollutant in water resources has become a rising worldwide concern. In this study, multi-functional nitrogen-doped carbon-MoS2 (NC-MoS2) nanohybrid composite was successfully synthesized by a step-by-step approach and applied as an effective heavy metal ions adsorbent. Scanning electron microscopy and energy dispersive X-ray spectrometer analysis presents multilayer irregular nanosheets and reveals the uniform distribution of C, N and O elements in the fabricated nanomaterials, respectively. Powder X-ray diffraction, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirm the functionalization of oxygen-containing functional groups arising from the nitrogen-doped carbon. Particularly, the NC-MoS2 possesses an excellent adsorption capacity (439.09mgg-1) for Pb(II), which attributes to the integrated physicochemical adsorption resulting from the oxygen-containing functional groups on the surface of nanohybrid composites. The as-prepared NC-MoS2 exhibits good chemical stability and can be reused 7 times with a slight fading over 80% during cycling, which is essential for its practical applications. All of these demonstrate that NC-MoS2 can be an efficient material for selectively removal of Pb(II), which suggest its great potential in the utilization of environmental cleanup.


Assuntos
Carbono/química , Chumbo/química , Nitrogênio/química , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/química , Adsorção , Microscopia Eletrônica de Varredura , Espectrometria por Raios X , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
15.
Inorg Chem ; 57(9): 5560-5566, 2018 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-29634244

RESUMO

We have demonstrated that natural sugars can efficiently exfoliate inorganic layered nanomaterials with direct stirring. The representative transition-metal dichalcogenides (MoS2 and WS2), transition-metal oxide (MoO3), and graphene were explored, and the formation of ultrathin nanosheets was verified. Glucose and MoS2 selected each other as the perfect partner with superior exfoliation and excellent properties. The obtained inorganic layered nanosheets possess favorable stability and dispersity, which renders it suitable for direct homogeneous liquid applications, such as catalytic activities and sensors. With a high-throughput and green process, the sugar-assisted method may offer new ideas for inorganic layered nanomaterials synthesis and applications in a more ecofriendly way.

16.
Anal Bioanal Chem ; 410(13): 3161-3170, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29594429

RESUMO

Small molecules are difficult to detect by conventional gold lateral flow assay (GLFA) sensitively because the test system must satisfy the conflict requirements between enough signal intensity and limited antibody (Ab) amount. In this work, a paired labels recognition (PLR)-based biosensor was designed by utilizing the specific binding of Ab and secondary antibody (anti-Ab) to enhance signal intensity and reduce antibody amount applied in small molecule detection. The PLR amplification system is fabricated by self-assembling the common detection probe, Au-labeled Ab (Au-Ab), and the signal booster, Au-labeled anti-Ab (Au-anti-Ab). Benefiting from this, a powerful network structure can be generated to accumulate numerous gold nanoparticles (GNPs) and thus significantly strengthen the signal intensity of detection. Therefore, a lower Ab amount will be applied to offer adequate signal strength, and further, the limit of detection will be obviously downregulated due to the more effective competition reaction. Using furazolidone (FZD) as a model analyte, we achieve a detection limit of as low as 1 ng mL-1, which was at least fivefold improved over that of the traditional GLFA. Furthermore, the practicality of this strategy was certificated in five different food samples. Graphical abstract A paired labels recognition (PLR) amplification system is fabricated by self-assembling the common detection probe, Au-labeled Ab (Au-Ab), and the signal booster, Au-labeled anti-Ab (Au-anti-Ab). In this novel strategy, owing to the recognition of both Ab and anti-Ab labeled on gold nanoparticles (GNPs), a powerful network structure can be generated to accumulate numerous GNPs and thus significantly strengthen the signal intensity of detection.


Assuntos
Anti-Infecciosos Locais/análise , Técnicas Biossensoriais/instrumentação , Análise de Alimentos/instrumentação , Furazolidona/análise , Ouro/química , Nanopartículas Metálicas/química , Fitas Reagentes/análise , Animais , Anticorpos Imobilizados/química , Galinhas , Desenho de Equipamento , Limite de Detecção , Carne/análise , Leite/química , Penaeidae , Carne Vermelha/análise , Frutos do Mar/análise , Suínos
17.
Nanotechnology ; 28(22): 225101, 2017 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-28480869

RESUMO

The emergence of antibiotic resistance has resulted in increasing difficulty in treating clinical infections associated with biofilm formation, one of the key processes in turn contributing to enhanced antibiotic resistance. With the rapid development of nanotechnology, a new way to overcome antibiotic resistance has opened up. Based on the many and diverse properties of MoS2 nanosheets that have attracted wide attention, in particular their antibacterial potential, herein, a novel antimicrobial agent to combat resistant gram-positive Staphylococcus aureus and gram-negative Salmonella was prepared using chitosan functionalized MoS2 nanosheets loading tetracycline hydrochloride drugs (abbreviated to CM-TH). The antibacterial and anti-biofilm activities of the CM-TH nanocomposites showed the synergetic effect that the combination of nanomaterials and antibiotics was more efficient than either working alone. In particularly, the minimum inhibitory concentration values generally decreased by a factor of dozens, suggesting that CM-TH may become a possible alternative to traditional antibiotics in disrupting biofilms and overcoming antibiotic resistance in treating medical diseases.

18.
ACS Appl Mater Interfaces ; 9(23): 19900-19907, 2017 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-28537405

RESUMO

Electrochemical conversion reactions of metal oxides provide a new avenue to build high capacity anodes for sodium-ion batteries. However, the poor rate performance and cyclability of these conversion anodes remain a significant challenge for Na-ion battery applications because most of the conversion anodes suffer from sluggish kinetics and irreversible structural change during cycles. In this paper, we report an Fe2O3 single crystallites/reduced graphene oxide composite (Fe2O3/rGO), where the Fe2O3 single crystallites with a particle size of ∼300 nm were uniformly anchored on the rGO nanosheets, which provide a highly conductive framework to facilitate electron transport and a flexible matrix to buffer the volume change of the material during cycling. This Fe2O3/rGO composite anode shows a very high reversible capacity of 610 mAh g-1 at 50 mA g-1, a high Coulombic efficiency of 71% at the first cycle, and a strong cyclability with 82% capacity retention after 100 cycles, suggesting a potential feasibility for sodium-ion batteries. More significantly, the present work clearly illustrates that an electrochemical conversion anode can be made with high capacity utilization, strong rate capability, and stable cyclability through appropriately tailoring the lattice structure, particle size, and electronic conduction channels for a simple transition-metal oxide, thus offering abundant selections for development of low-cost and high-performance Na-storage electrodes.

19.
Nanotechnology ; 2017 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-28398220

RESUMO

The emergence of antibiotic resistance has resulted in an increasing difficulty treating clinical infections associated with biofilms formation, one of the key processes contributed to enhance antibiotic resistance in return. With the rapid development of nanotechnology, a new way to overcome antibiotic resistance was opened up. Based on multiple properties especially antibacterial potential of MoS2 nanosheets that have aroused wide attention, herein, a novel antimicrobial agent to combat resistant gram-positive Staphylococcus aureus (S. aureus) and gram-negative Salmonella was prepared using chitosan functionalized MoS2 nanosheets loading tetracycline hydrochloride drugs (abbreviated to CM-TH). The antibacterial and anti-biofilm activities of CM-TH nanocomposites expressed a synergy effect that the combination of nanomaterials and antibiotics were more efficient than both alone did. Particularly, the MIC values were generally decreased by a factor of dozens, suggesting CM-TH may become a possible alternative to traditional antibiotics in disrupting the biofilms and further to overcome antibiotic resistance in treating medical diseases.

20.
Anal Biochem ; 525: 100-106, 2017 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-28263739

RESUMO

Microfluidic paper-based analytical devices (µPADs) have a significant potential in developing portable and disposable point-of-care testing (POCT). Herein, a facile, rapid, cost-effective and environment friendly strategy for µPADs fabrication is proposed. Specifically, the substrate paper was hydrophobized by coating with trimethoxysilane (TOS), and then the selected area was hydrophilized by treating with surfactant. The whole fabrication process was implemented within 7 min, with no need for complex pre-treatment, high-temperature and special equipment. As a proof-of-concept application, the as-prepared µPAD was applied to determination of the glucose content in human serum samples. The results agreed well with those obtained by a glucometer. We believe that the µPADs fabrication method proposed here could provide a facile, rapid and low-cost reference for other related studies.


Assuntos
Glicemia/análise , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Papel , Desenho de Equipamento , Humanos , Interações Hidrofóbicas e Hidrofílicas , Sistemas Automatizados de Assistência Junto ao Leito
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